FvH4_4g19730

Iron-Sulfur binding protein C terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014717
Physical Location & Seq
Forward (+)
23275998 .. 23281031
5034 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19730.t7

Sequence Viewer

Length: 1254 bp
ATGGCACTGAGCTTCTTCTCCTGTTGTGCTTCAATTGCTCCTCAACAGCATCAAGCAAAACTGAACCTTAGAGGCAACAAAGCCCTTGAAGATGTGAAGAGCCTCATCAAAACTGTTGGGGTTCCATCAGTTGTGTCCTCTCCAGTAAAATCTCTTCAGAATGGCAACTGGGTCAAGCTCATATGCGGCGCAAGCTTCGAGGATGTGGTTGATATACGGAATCTTTCTTTGGTTTACACTCTTGCTGGAGTGGATTGCATAGACTGTGCGGCAGATGTATCGGTGGTGAGCGCGGTAAATGAGGGGATTGATGCAGCATTGGAGATTTTCCCTTTTCGAAGGCCTTGGGTGATGATCAGTGTAAATGATGATGAGGATCTTCACTTTCGAAAAGCTGAATTTGATCCAGAGGAGTGTCCACTTGACTGTTCAAGGCCTTGTGAGAGAGTATGTCCTGCTGATGCAATATCTTTTGAGTCTGGATGGTCAACAACAAAATTCTCGTATGGCAACAAAACACCGACCTCAGTAAAGGCTGGAGTATTAACTGAACGCTGCTATGGCTGTGGCCGCTGCCTTCCAGTTTGCCCATACGATAAAATAAGTGTGAGCACATACATAAGAGATACTGCGGCAACCTCTGAACTTCTCAAAAGAAATGATGTTCATGCTATAGAGATTCATACAAGTGGAAGGCAGATTGCTCCATTTAAAGAACTTTGGGATGGTTTGGGAGGTTCAATCAAACATCTTAAACTAGCAGCAGTTAGTTTACCTGATCATGGAGAGTCAACTGTATCAACAATGAAGGCTATGTACTCCATTATGGAACCGGATTTATCTTGCTTCAATTTATGGCAGTTGGATGGGCGCCCCATGAGCGGAGATATTGGTCGAGGTGCCACAAGAGAATCAATCGCTTTTGCTCTTCGTTTAGCTGCTATAAAAGAAAGGCCTCATGGTTTTCTTCAACTGGCCGGTGGCACCAATGCTCACACAGTTGATGGGTTGAGGAAAGTTGGACTTTTTCAAGCGACATCCTTTACAAAGAACTCGAGGGATGAAAACTCAACAGCTATGATTGGTGGTGTTGCTTATGGTGGCTATGCACGAAAGATTGTTGGAAGAATCCTGAGTTCAATGCAATCACAGCACGGCATTACTGGGATTGAGGATTATCCAGAGCATCTCCTTCAGGCGCTTAGAGAGGCCCTAGCTTTAGTTGGAAAACTCAAATGTTACCAGTCGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4 Fer4_9 LdpA_C

Protein Analysis

418

Amino Acids

45.36

Weight (kDa)

6.56

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LdpA_Fe-S-bd PF25160 18 - 85 5.4e-22 LdpA Fe-S cluster-binding domain
Fer4_9 PF13187 21 - 78 4e-06 4Fe-4S dicluster domain
Fer4_7 PF12838 22 - 78 4.5e-07 4Fe-4S dicluster domain
LdpA_C PF12617 95 - 288 2.4e-63 Light dependent period A C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014717)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g19730 FvH4_4g19730 FvH4_4g19730 FvH4_4g19730 FvH4_4g19730
malus_domestica MD16G1154200.v1.1
prunus_persica Prupe.1G117500_v2.0.a1 Prupe.1G117500_v2.0.a1
pyrus_communis pycom16g13060
rosa_chinensis RchiOBHm_Chr4g0425231
rosa_laevigata RLG00000007384
rosa_multiflora Rmu_sc0004716.1_g000005
rosa_roxburghii Rroxscaffold_5G00367260
rosa_rugosa Rorug04G0202000
rosa_samantha Rh4BG264400 Rh4DG260500
rosa_wichuraiana Rw4G022410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 870, 899, 983
AccBSI CCGCTC 1 cut(s) 882
AccII CGCG 1 cut(s) 293
AciI CCGC 6 cut(s) 186, 269, 293, 571, 632, 882
AclWI GGATC 2 cut(s) 384, 398
AcoI YGGCCR 2 cut(s) 568, 975
AcsI RAATTY 2 cut(s) 398, 497
AcuI CTGAAG 2 cut(s) 140, 1178
AcyI GRCGYC 1 cut(s) 871
AfaI GTAC 1 cut(s) 818
AfiI CCNNNNNNNGG 2 cut(s) 782, 881
AgsI TTSAA 8 cut(s) 33, 89, 432, 741, 850, 971, 1031, 1140
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
AluBI AGCT 7 cut(s) 12, 178, 195, 395, 938, 1076, 1217
AluI AGCT 7 cut(s) 12, 178, 195, 395, 938, 1076, 1217
Alw21I GWGCWC 1 cut(s) 614
AlwI GGATC 2 cut(s) 384, 398
Ama87I CYCGRG 1 cut(s) 1054
AoxI GGCC 6 cut(s) 341, 434, 568, 953, 975, 1209
ApeKI GCWGC 5 cut(s) 314, 555, 573, 761, 938
ApoI RAATTY 2 cut(s) 398, 497
AspLEI GCGC 4 cut(s) 191, 293, 873, 1201
AspS9I GGNCC 1 cut(s) 1210
AsuHPI GGTGA 2 cut(s) 298, 361
AsuII TTCGAA 2 cut(s) 337, 388
AvaI CYCGRG 1 cut(s) 1054
BanI GGYRCC 3 cut(s) 870, 899, 983
BarI GAAGNNNNNNTAC 4 cut(s) 138, 170, 800, 832
Bbv12I GWGCWC 1 cut(s) 614
BbvI GCAGC 5 cut(s) 326, 542, 560, 773, 925
BccI CCATC 5 cut(s) 133, 477, 719, 860, 998
BceAI ACGGC 1 cut(s) 1171
BcgI CGANNNNNNTGC 2 cut(s) 261, 295
BclI TGATCA 2 cut(s) 354, 778
BfaI CTAG 3 cut(s) 758, 1214, 1252
BfmI CTRYAG 1 cut(s) 672
BfoI RGCGCY 2 cut(s) 874, 1202
BisI GCNGC 9 cut(s) 187, 270, 315, 556, 571, 574, 633, 762, 939
BlsI GCNGC 9 cut(s) 188, 271, 316, 557, 572, 575, 634, 763, 940
BmeT110I CYCGRG 1 cut(s) 1054
BmgT120I GGNCC 1 cut(s) 1210
BmiI GGNNCC 5 cut(s) 123, 831, 872, 901, 985
BmrI ACTGGG 2 cut(s) 178, 1173
BmsI GCATC 4 cut(s) 58, 301, 451, 1195
BmuI ACTGGG 2 cut(s) 178, 1173
BpmI CTGGAG 3 cut(s) 126, 267, 558
Bpu14I TTCGAA 2 cut(s) 337, 388
BsaBI GATNNNNATC 1 cut(s) 375
BsaHI GRCGYC 1 cut(s) 871
BsaJI CCNNGG 1 cut(s) 344
BsaWI WCCGGW 1 cut(s) 832
Bsc4I CCNNNNNNNGG 2 cut(s) 782, 881
Bse118I RCCGGY 1 cut(s) 977
Bse1I ACTGG 6 cut(s) 143, 173, 581, 978, 1168, 1243
Bse8I GATNNNNATC 1 cut(s) 375
BseDI CCNNGG 1 cut(s) 344
BseGI GGATG 6 cut(s) 208, 488, 730, 871, 1037, 1066
BseJI GATNNNNATC 1 cut(s) 375
BseLI CCNNNNNNNGG 2 cut(s) 782, 881
BseMII CTCAG 2 cut(s) 540, 1124
BseNI ACTGG 6 cut(s) 143, 173, 581, 978, 1168, 1243
BseRI GAGGAG 2 cut(s) 30, 425
BseXI GCAGC 5 cut(s) 326, 542, 560, 773, 925
Bsh1236I CGCG 1 cut(s) 293
BshFI GGCC 6 cut(s) 343, 436, 570, 955, 977, 1211
BshNI GGYRCC 3 cut(s) 870, 899, 983
BsiHKAI GWGCWC 1 cut(s) 614
BsiHKCI CYCGRG 1 cut(s) 1054
BsiSI CCGG 2 cut(s) 833, 978
BslI CCNNNNNNNGG 2 cut(s) 782, 881
BsnI GGCC 6 cut(s) 343, 436, 570, 955, 977, 1211
BsoBI CYCGRG 1 cut(s) 1054
Bsp119I TTCGAA 2 cut(s) 337, 388
Bsp1286I GDGCHC 1 cut(s) 614
Bsp143I GATC 4 cut(s) 354, 376, 403, 778
BspACI CCGC 6 cut(s) 186, 269, 293, 571, 632, 882
BspANI GGCC 6 cut(s) 343, 436, 570, 955, 977, 1211
BspCNI CTCAG 2 cut(s) 539, 1125
BspFNI CGCG 1 cut(s) 293
BspLI GGNNCC 5 cut(s) 123, 831, 872, 901, 985
BspPI GGATC 2 cut(s) 384, 398
BspQI GCTCTTC 2 cut(s) 92, 933
BspT104I TTCGAA 2 cut(s) 337, 388
BspT107I GGYRCC 3 cut(s) 870, 899, 983
BsrBI CCGCTC 1 cut(s) 882
BsrFI RCCGGY 1 cut(s) 977
BsrI ACTGG 6 cut(s) 143, 173, 581, 978, 1168, 1243
BssAI RCCGGY 1 cut(s) 977
BssECI CCNNGG 1 cut(s) 344
BssMI GATC 4 cut(s) 354, 376, 403, 778
BssNI GRCGYC 1 cut(s) 871
BssT1I CCWWGG 1 cut(s) 344
Bst4CI ACNGT 5 cut(s) 115, 266, 428, 796, 1000
Bst6I CTCTTC 3 cut(s) 92, 159, 933
BstACI GRCGYC 1 cut(s) 871
BstBI TTCGAA 2 cut(s) 337, 388
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 5 cut(s) 8, 68, 526, 1133, 1202
BstF5I GGATG 6 cut(s) 208, 488, 730, 871, 1037, 1066
BstFNI CGCG 1 cut(s) 293
BstH2I RGCGCY 2 cut(s) 874, 1202
BstHHI GCGC 4 cut(s) 191, 293, 873, 1201
BstKTI GATC 4 cut(s) 357, 379, 406, 781
BstMBI GATC 4 cut(s) 354, 376, 403, 778
BstMWI GCNNNNNNNGC 6 cut(s) 35, 192, 561, 570, 879, 1150
BstSFI CTRYAG 1 cut(s) 672
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 5 cut(s) 326, 542, 560, 773, 925
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BsuRI GGCC 6 cut(s) 343, 436, 570, 955, 977, 1211
BtsCI GGATG 6 cut(s) 208, 488, 730, 871, 1037, 1066
BtsIMutI CAGTG 2 cut(s) 5, 364
Cac8I GCNNGC 1 cut(s) 193
CfoI GCGC 4 cut(s) 191, 293, 873, 1201
Cfr10I RCCGGY 1 cut(s) 977
Cfr13I GGNCC 1 cut(s) 1210
Csp6I GTAC 1 cut(s) 817
CviAII CATG 4 cut(s) 668, 782, 877, 959
CviQI GTAC 1 cut(s) 817
DdeI CTNAG 5 cut(s) 8, 68, 526, 1133, 1202
DinI GGCGCC 1 cut(s) 872
DpnI GATC 4 cut(s) 356, 378, 405, 780
DpnII GATC 4 cut(s) 354, 376, 403, 778
DraI TTTAAA 1 cut(s) 712
EaeI YGGCCR 2 cut(s) 568, 975
Eam1104I CTCTTC 3 cut(s) 92, 159, 933
EarI CTCTTC 3 cut(s) 92, 159, 933
Eco130I CCWWGG 1 cut(s) 344
Eco147I AGGCCT 3 cut(s) 343, 436, 955
Eco57I CTGAAG 2 cut(s) 140, 1178
Eco88I CYCGRG 1 cut(s) 1054
EcoO109I RGGNCCY 1 cut(s) 1210
EcoT14I CCWWGG 1 cut(s) 344
EgeI GGCGCC 1 cut(s) 872
EheI GGCGCC 1 cut(s) 872
ErhI CCWWGG 1 cut(s) 344
FaeI CATG 4 cut(s) 671, 785, 880, 962
FalI AAGNNNNNCTT 2 cut(s) 1008, 1040
FatI CATG 4 cut(s) 667, 781, 876, 958
FauNDI CATATG 1 cut(s) 182
FbaI TGATCA 2 cut(s) 354, 778
Fnu4HI GCNGC 9 cut(s) 187, 270, 315, 556, 571, 574, 633, 762, 939
FokI GGATG 6 cut(s) 215, 495, 737, 878, 1024, 1073
Fsp4HI GCNGC 9 cut(s) 187, 270, 315, 556, 571, 574, 633, 762, 939
FspBI CTAG 3 cut(s) 758, 1214, 1252
GlaI GCGC 4 cut(s) 190, 292, 872, 1200
GluI GCNGC 9 cut(s) 187, 270, 315, 556, 571, 574, 633, 762, 939
GsuI CTGGAG 3 cut(s) 126, 267, 558
HaeII RGCGCY 2 cut(s) 874, 1202
HaeIII GGCC 6 cut(s) 343, 436, 570, 955, 977, 1211
HapII CCGG 2 cut(s) 833, 978
HhaI GCGC 4 cut(s) 191, 293, 873, 1201
Hin1I GRCGYC 1 cut(s) 871
Hin1II CATG 4 cut(s) 671, 785, 880, 962
Hin6I GCGC 4 cut(s) 189, 291, 871, 1199
HinP1I GCGC 4 cut(s) 189, 291, 871, 1199
HincII GTYRAC 2 cut(s) 489, 792
HindII GTYRAC 2 cut(s) 489, 792
HindIII AAGCTT 1 cut(s) 193
HinfI GANTC 6 cut(s) 220, 476, 679, 788, 911, 1128
HpaII CCGG 2 cut(s) 833, 978
HphI GGTGA 2 cut(s) 298, 361
Hpy166II GTNNAC 5 cut(s) 235, 419, 489, 773, 792
Hpy188I TCNGA 2 cut(s) 159, 643
Hpy188III TCNNGA 4 cut(s) 407, 480, 1132, 1181
Hpy8I GTNNAC 5 cut(s) 235, 419, 489, 773, 792
HpyAV CCTTC 5 cut(s) 333, 587, 687, 802, 1202
HpyCH4III ACNGT 5 cut(s) 115, 266, 428, 796, 1000
HpyCH4V TGCA 5 cut(s) 258, 314, 464, 1109, 1144
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 192, 561, 570, 879, 1150
HpyF3I CTNAG 5 cut(s) 8, 68, 526, 1133, 1202
Hsp92I GRCGYC 1 cut(s) 871
Hsp92II CATG 4 cut(s) 671, 785, 880, 962
HspAI GCGC 4 cut(s) 189, 291, 871, 1199
KasI GGCGCC 1 cut(s) 870
Ksp22I TGATCA 2 cut(s) 354, 778
Kzo9I GATC 4 cut(s) 354, 376, 403, 778
LguI GCTCTTC 2 cut(s) 92, 933
LmnI GCTCC 2 cut(s) 43, 709
Lsp1109I GCAGC 5 cut(s) 326, 542, 560, 773, 925
LweI GCATC 4 cut(s) 58, 301, 451, 1195
MaeI CTAG 3 cut(s) 758, 1214, 1252
MaeIII GTNAC 1 cut(s) 1238
MalI GATC 4 cut(s) 356, 378, 405, 780
MbiI CCGCTC 1 cut(s) 882
MboI GATC 4 cut(s) 354, 376, 403, 778
MboII GAAGA 8 cut(s) 7, 101, 109, 146, 371, 920, 959, 1137
MfeI CAATTG 1 cut(s) 33
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 1 cut(s) 614
MluCI AATT 4 cut(s) 33, 398, 497, 850
Mly113I GGCGCC 1 cut(s) 871
MlyI GAGTC 2 cut(s) 485, 797
MmeI TCCRAC 4 cut(s) 843, 1000, 1102, 1204
MseI TTAA 3 cut(s) 545, 711, 753
MslI CAYNNNNRTG 1 cut(s) 687
MspA1I CMGCKG 1 cut(s) 573
MspI CCGG 2 cut(s) 833, 978
MunI CAATTG 1 cut(s) 33
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 6 cut(s) 35, 192, 561, 570, 879, 1150
NarI GGCGCC 1 cut(s) 871
NdeI CATATG 1 cut(s) 182
NdeII GATC 4 cut(s) 354, 376, 403, 778
NlaIII CATG 4 cut(s) 671, 785, 880, 962
NlaIV GGNNCC 5 cut(s) 123, 831, 872, 901, 985
NspV TTCGAA 2 cut(s) 337, 388
PaeR7I CTCGAG 1 cut(s) 1054
PceI AGGCCT 3 cut(s) 343, 436, 955
PciSI GCTCTTC 2 cut(s) 92, 933
PfeI GAWTC 4 cut(s) 220, 679, 911, 1128
PkrI GCNGC 9 cut(s) 188, 271, 316, 557, 572, 575, 634, 763, 940
PleI GAGTC 2 cut(s) 484, 796
PluTI GGCGCC 1 cut(s) 874
PpsI GAGTC 2 cut(s) 484, 796
PspN4I GGNNCC 5 cut(s) 123, 831, 872, 901, 985
PspPI GGNCC 1 cut(s) 1210
PspXI VCTCGAGB 1 cut(s) 1054
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 1 cut(s) 818
RsaNI GTAC 1 cut(s) 817
RseI CAYNNNNRTG 1 cut(s) 687
SapI GCTCTTC 2 cut(s) 92, 933
SaqAI TTAA 3 cut(s) 545, 711, 753
SatI GCNGC 9 cut(s) 187, 270, 315, 556, 571, 574, 633, 762, 939
Sau3AI GATC 4 cut(s) 354, 376, 403, 778
Sau96I GGNCC 1 cut(s) 1210
SchI GAGTC 2 cut(s) 485, 797
SduI GDGCHC 1 cut(s) 614
SfaNI GCATC 4 cut(s) 58, 301, 451, 1195
SfcI CTRYAG 1 cut(s) 672
SfoI GGCGCC 1 cut(s) 872
Sfr274I CTCGAG 1 cut(s) 1054
SfuI TTCGAA 2 cut(s) 337, 388
SlaI CTCGAG 1 cut(s) 1054
SmiMI CAYNNNNRTG 1 cut(s) 687
SmlI CTYRAG 1 cut(s) 1054
SmoI CTYRAG 1 cut(s) 1054
Sse9I AATT 4 cut(s) 33, 398, 497, 850
SseBI AGGCCT 3 cut(s) 343, 436, 955
SsiI CCGC 6 cut(s) 186, 269, 293, 571, 632, 882
SspDI GGCGCC 1 cut(s) 870
SspMI CTAG 3 cut(s) 758, 1214, 1252
StuI AGGCCT 3 cut(s) 343, 436, 955
StyI CCWWGG 1 cut(s) 344
TaaI ACNGT 5 cut(s) 115, 266, 428, 796, 1000
TaqI TCGA 5 cut(s) 198, 337, 388, 895, 1055
TasI AATT 4 cut(s) 33, 398, 497, 850
TatI WGTACW 1 cut(s) 816
TauI GCSGC 4 cut(s) 189, 272, 573, 635
TfiI GAWTC 4 cut(s) 220, 679, 911, 1128
Tru1I TTAA 3 cut(s) 545, 711, 753
Tru9I TTAA 3 cut(s) 545, 711, 753
TscAI CASTG 2 cut(s) 12, 364
TseI GCWGC 5 cut(s) 314, 555, 573, 761, 938
TspDTI ATGAA 4 cut(s) 656, 671, 821, 1077
TspGWI ACGGA 1 cut(s) 232
TspRI CASTG 2 cut(s) 12, 364
XapI RAATTY 2 cut(s) 398, 497
XhoI CTCGAG 1 cut(s) 1054
XspI CTAG 3 cut(s) 758, 1214, 1252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.