Rw4G022410

Iron-Sulfur binding protein C terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014717
Physical Location & Seq
Forward (+)
47769365 .. 47772077
2713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G022410.1

Sequence Viewer

Length: 1248 bp
ATGGCACTGAGCTTCTTCTCCTGTTGTGCTTCAATTCCTCCTCAACAACATCAAGCAAAACTGAACCTTGGAGTCAACAAAGCCCTTGAAGAGGTAAGGAGCCTCATAAAAACTGTTGGGGTTCCATCAGTTGCGTCCTCTCCAGTAAAATCACTTCAAAATGGCAACTGGGTCAAGCTCATATGCGGCGCAAGCTTCGAGGACGTGGTTGATATAAGGAATCTCTCTCTAGTTTACTCTCTTGCAGGAGTGGATTGCATAGACTGTGCAGCAGATGTATCAGTGGTCAGCGCGGTAAATGAGGGGATTGATGCAGCATTGGAGATTTTCCCTCTTCGAAGGCCTTGGGTGATGATCAGTGTAAATGATGATGAGGATCTTCACTTTCGAAAAGCTGAATTTGATCCAGAGGATTGTCCACTTGACTGTTCAAGGCCTTGTGAGAGTGTATGTCCTGCTGATGCAATATCTTTTGAAACTGGAAGATCAATAACACAATTCGGCACCAAAACATCTGCCTCATTAAAGGCCGGAGTTCTAACCGAACGCTGCTATGGCTGTGGCCGCTGCATTCCAGTTTGCCCATACGATAAAATAAGTGTGAGCACATACATAAGAGATGCTACTGCAACCTCTGAACTTCTGAAAAGAAATGATGTTGATGCTATAGAGATTCATACAAGTGGAAGGCAGATTGCTCCATTTAAAAAACTTTGGGATGGTTTGGGAGATTCAATCAAACATCTCAGACTAGCAGCAGTTAGTTTACCTGATCTTGAAGAATCAACCGTATCAACAATGAAGGCTATGTACTCTATTATGGAACCAGATTTATGTAGCTTTAATTTATGGCAGTTGGATGGCCGCCCCATGAGCGGAGATATTGGTCGAGGTGCCACAAGAGAATCAATTGCTTTTGCTCTTCGTTTGGCTGCTATAAAAGAAAGGCCTCATGGTTTTCTTCAATTAGCCGGTGGCACCAATGCTCACACAGTTGATGGGTTGAGGAAAGTCGGACTTTTTCAAGCGACATCCTTTACAAAGAACTCGAGGAATGAAAACTCAACAGCTATGATTGGTGGTGTTGCTTATGGTGGCTATGCACGAAAGATTGTTGGAAGAATCCTGAGTTCTATGCAATCACAAGACGGCATTACTGGGATTGAGGATTATCCAGAGCATCTCCTTCAAGCACTTAGAGAGGCCCTAGCTTTAGTTGGAAAACTCAAATGTTACCGGTCCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4 Fer4_9 LdpA_C

Protein Analysis

415

Amino Acids

45.01

Weight (kDa)

6.03

Isoelectric Point (pI)

39.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LdpA_Fe-S-bd PF25160 139 - 204 1.3e-21 LdpA Fe-S cluster-binding domain
Fer4_9 PF13187 142 - 197 2.5e-06 4Fe-4S dicluster domain
LdpA_C PF12617 214 - 407 3.3e-62 Light dependent period A C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014717)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g19730 FvH4_4g19730 FvH4_4g19730 FvH4_4g19730 FvH4_4g19730
malus_domestica MD16G1154200.v1.1
prunus_persica Prupe.1G117500_v2.0.a1 Prupe.1G117500_v2.0.a1
pyrus_communis pycom16g13060
rosa_chinensis RchiOBHm_Chr4g0425231
rosa_laevigata RLG00000007384
rosa_multiflora Rmu_sc0004716.1_g000005
rosa_roxburghii Rroxscaffold_5G00367260
rosa_rugosa Rorug04G0202000
rosa_samantha Rh4BG264400 Rh4DG260500
rosa_wichuraiana Rw4G022410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 503, 893, 977
AccBSI CCGCTC 1 cut(s) 876
AccII CGCG 1 cut(s) 293
AciI CCGC 5 cut(s) 186, 293, 565, 865, 876
AclWI GGATC 2 cut(s) 384, 398
AcoI YGGCCR 2 cut(s) 562, 862
AcsI RAATTY 1 cut(s) 398
AfaI GTAC 1 cut(s) 812
AfiI CCNNNNNNNGG 2 cut(s) 91, 875
AgeI ACCGGT 1 cut(s) 1236
AjiI CACGTC 1 cut(s) 205
AluBI AGCT 7 cut(s) 12, 178, 195, 395, 840, 1070, 1211
AluI AGCT 7 cut(s) 12, 178, 195, 395, 840, 1070, 1211
Alw21I GWGCWC 1 cut(s) 608
AlwI GGATC 2 cut(s) 384, 398
Ama87I CYCGRG 1 cut(s) 1048
AoxI GGCC 7 cut(s) 341, 434, 528, 562, 862, 947, 1203
ApeKI GCWGC 6 cut(s) 269, 314, 549, 567, 755, 932
ApoI RAATTY 1 cut(s) 398
AsiGI ACCGGT 1 cut(s) 1236
AspLEI GCGC 2 cut(s) 191, 293
AspS9I GGNCC 2 cut(s) 1204, 1239
AsuHPI GGTGA 1 cut(s) 361
AsuII TTCGAA 2 cut(s) 337, 388
AvaI CYCGRG 1 cut(s) 1048
AvaII GGWCC 1 cut(s) 1239
BanI GGYRCC 3 cut(s) 503, 893, 977
BarI GAAGNNNNNNTAC 4 cut(s) 138, 170, 794, 826
Bbv12I GWGCWC 1 cut(s) 608
BbvI GCAGC 6 cut(s) 281, 326, 536, 554, 767, 919
BccI CCATC 4 cut(s) 133, 713, 854, 992
BceAI ACGGC 1 cut(s) 1165
BclI TGATCA 1 cut(s) 354
BfaI CTAG 3 cut(s) 230, 752, 1208
BfmI CTRYAG 1 cut(s) 666
BisI GCNGC 9 cut(s) 187, 270, 315, 550, 565, 568, 756, 865, 933
BlsI GCNGC 9 cut(s) 188, 271, 316, 551, 566, 569, 757, 866, 934
Bme18I GGWCC 1 cut(s) 1239
BmeT110I CYCGRG 1 cut(s) 1048
BmgBI CACGTC 1 cut(s) 205
BmgT120I GGNCC 2 cut(s) 1204, 1239
BmiI GGNNCC 6 cut(s) 101, 123, 505, 825, 895, 979
BmrI ACTGGG 2 cut(s) 178, 1167
BmsI GCATC 5 cut(s) 301, 451, 610, 652, 1189
BmuI ACTGGG 2 cut(s) 178, 1167
BpmI CTGGAG 1 cut(s) 126
Bpu14I TTCGAA 2 cut(s) 337, 388
BsaBI GATNNNNATC 1 cut(s) 375
BsaJI CCNNGG 2 cut(s) 67, 344
BsaWI WCCGGW 1 cut(s) 1236
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 875
Bse118I RCCGGY 2 cut(s) 971, 1236
Bse1I ACTGG 5 cut(s) 143, 173, 484, 575, 1162
Bse8I GATNNNNATC 1 cut(s) 375
BseDI CCNNGG 2 cut(s) 67, 344
BseGI GGATG 3 cut(s) 724, 865, 1031
BseJI GATNNNNATC 1 cut(s) 375
BseLI CCNNNNNNNGG 2 cut(s) 91, 875
BseMII CTCAG 2 cut(s) 760, 1118
BseNI ACTGG 5 cut(s) 143, 173, 484, 575, 1162
BseRI GAGGAG 1 cut(s) 30
BseXI GCAGC 6 cut(s) 281, 326, 536, 554, 767, 919
BsgI GTGCAG 1 cut(s) 288
Bsh1236I CGCG 1 cut(s) 293
BshFI GGCC 7 cut(s) 343, 436, 530, 564, 864, 949, 1205
BshNI GGYRCC 3 cut(s) 503, 893, 977
BshTI ACCGGT 1 cut(s) 1236
BsiHKAI GWGCWC 1 cut(s) 608
BsiHKCI CYCGRG 1 cut(s) 1048
BsiSI CCGG 3 cut(s) 531, 972, 1237
BslI CCNNNNNNNGG 2 cut(s) 91, 875
BsmI GAATGC 1 cut(s) 570
BsnI GGCC 7 cut(s) 343, 436, 530, 564, 864, 949, 1205
BsoBI CYCGRG 1 cut(s) 1048
Bsp119I TTCGAA 2 cut(s) 337, 388
Bsp1286I GDGCHC 1 cut(s) 608
Bsp143I GATC 5 cut(s) 354, 376, 403, 485, 772
BspACI CCGC 5 cut(s) 186, 293, 565, 865, 876
BspANI GGCC 7 cut(s) 343, 436, 530, 564, 864, 949, 1205
BspCNI CTCAG 2 cut(s) 759, 1119
BspFNI CGCG 1 cut(s) 293
BspLI GGNNCC 6 cut(s) 101, 123, 505, 825, 895, 979
BspPI GGATC 2 cut(s) 384, 398
BspQI GCTCTTC 1 cut(s) 927
BspT104I TTCGAA 2 cut(s) 337, 388
BspT107I GGYRCC 3 cut(s) 503, 893, 977
BsrBI CCGCTC 1 cut(s) 876
BsrFI RCCGGY 2 cut(s) 971, 1236
BsrI ACTGG 5 cut(s) 143, 173, 484, 575, 1162
BssAI RCCGGY 2 cut(s) 971, 1236
BssECI CCNNGG 2 cut(s) 67, 344
BssMI GATC 5 cut(s) 354, 376, 403, 485, 772
BssT1I CCWWGG 2 cut(s) 67, 344
Bst4CI ACNGT 5 cut(s) 115, 266, 428, 790, 994
Bst6I CTCTTC 3 cut(s) 84, 339, 927
BstBI TTCGAA 2 cut(s) 337, 388
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 4 cut(s) 8, 746, 1127, 1196
BstENI CCTNNNNNAGG 1 cut(s) 89
BstF5I GGATG 3 cut(s) 724, 865, 1031
BstFNI CGCG 1 cut(s) 293
BstHHI GCGC 2 cut(s) 191, 293
BstKTI GATC 5 cut(s) 357, 379, 406, 488, 775
BstMBI GATC 5 cut(s) 354, 376, 403, 485, 772
BstMWI GCNNNNNNNGC 4 cut(s) 192, 555, 564, 873
BstSFI CTRYAG 1 cut(s) 666
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 6 cut(s) 281, 326, 536, 554, 767, 919
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BsuRI GGCC 7 cut(s) 343, 436, 530, 564, 864, 949, 1205
BtrI CACGTC 1 cut(s) 205
BtsCI GGATG 3 cut(s) 724, 865, 1031
BtsIMutI CAGTG 3 cut(s) 5, 288, 364
Cac8I GCNNGC 1 cut(s) 193
CfoI GCGC 2 cut(s) 191, 293
Cfr10I RCCGGY 2 cut(s) 971, 1236
Cfr13I GGNCC 2 cut(s) 1204, 1239
CseI GACGC 1 cut(s) 123
Csp6I GTAC 1 cut(s) 811
CspAI ACCGGT 1 cut(s) 1236
CviAII CATG 2 cut(s) 871, 953
CviQI GTAC 1 cut(s) 811
DdeI CTNAG 4 cut(s) 8, 746, 1127, 1196
DpnI GATC 5 cut(s) 356, 378, 405, 487, 774
DpnII GATC 5 cut(s) 354, 376, 403, 485, 772
DraI TTTAAA 1 cut(s) 706
EaeI YGGCCR 2 cut(s) 562, 862
Eam1104I CTCTTC 3 cut(s) 84, 339, 927
EarI CTCTTC 3 cut(s) 84, 339, 927
Eco130I CCWWGG 2 cut(s) 67, 344
Eco147I AGGCCT 3 cut(s) 343, 436, 949
Eco47I GGWCC 1 cut(s) 1239
Eco88I CYCGRG 1 cut(s) 1048
EcoNI CCTNNNNNAGG 1 cut(s) 89
EcoO109I RGGNCCY 1 cut(s) 1204
EcoT14I CCWWGG 2 cut(s) 67, 344
ErhI CCWWGG 2 cut(s) 67, 344
FaeI CATG 2 cut(s) 874, 956
FalI AAGNNNNNCTT 2 cut(s) 1002, 1034
FatI CATG 2 cut(s) 870, 952
FauNDI CATATG 1 cut(s) 182
FbaI TGATCA 1 cut(s) 354
Fnu4HI GCNGC 9 cut(s) 187, 270, 315, 550, 565, 568, 756, 865, 933
FokI GGATG 3 cut(s) 731, 872, 1018
Fsp4HI GCNGC 9 cut(s) 187, 270, 315, 550, 565, 568, 756, 865, 933
FspBI CTAG 3 cut(s) 230, 752, 1208
GlaI GCGC 2 cut(s) 190, 292
GluI GCNGC 9 cut(s) 187, 270, 315, 550, 565, 568, 756, 865, 933
GsuI CTGGAG 1 cut(s) 126
HaeIII GGCC 7 cut(s) 343, 436, 530, 564, 864, 949, 1205
HapII CCGG 3 cut(s) 531, 972, 1237
HgaI GACGC 1 cut(s) 123
HhaI GCGC 2 cut(s) 191, 293
Hin1II CATG 2 cut(s) 874, 956
Hin6I GCGC 2 cut(s) 189, 291
HinP1I GCGC 2 cut(s) 189, 291
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HindIII AAGCTT 1 cut(s) 193
HinfI GANTC 7 cut(s) 72, 220, 673, 731, 782, 905, 1122
HpaII CCGG 3 cut(s) 531, 972, 1237
HphI GGTGA 1 cut(s) 361
Hpy166II GTNNAC 4 cut(s) 76, 235, 419, 767
Hpy188I TCNGA 4 cut(s) 637, 645, 749, 1016
Hpy188III TCNNGA 4 cut(s) 407, 776, 1126, 1175
Hpy8I GTNNAC 4 cut(s) 76, 235, 419, 767
HpyAV CCTTC 4 cut(s) 333, 681, 796, 1196
HpyCH4III ACNGT 5 cut(s) 115, 266, 428, 790, 994
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 9 cut(s) 245, 258, 269, 314, 464, 570, 629, 1103, 1138
HpyF10VI GCNNNNNNNGC 4 cut(s) 192, 555, 564, 873
HpyF3I CTNAG 4 cut(s) 8, 746, 1127, 1196
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 2 cut(s) 874, 956
HspAI GCGC 2 cut(s) 189, 291
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 5 cut(s) 354, 376, 403, 485, 772
LguI GCTCTTC 1 cut(s) 927
LmnI GCTCC 2 cut(s) 99, 703
Lsp1109I GCAGC 6 cut(s) 281, 326, 536, 554, 767, 919
LweI GCATC 5 cut(s) 301, 451, 610, 652, 1189
MaeI CTAG 3 cut(s) 230, 752, 1208
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 1232
MalI GATC 5 cut(s) 356, 378, 405, 487, 774
MbiI CCGCTC 1 cut(s) 876
MboI GATC 5 cut(s) 354, 376, 403, 485, 772
MboII GAAGA 9 cut(s) 7, 101, 326, 371, 495, 791, 914, 953, 1131
MfeI CAATTG 1 cut(s) 909
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 1 cut(s) 608
MluCI AATT 6 cut(s) 33, 398, 497, 844, 909, 965
MlyI GAGTC 1 cut(s) 81
MmeI TCCRAC 4 cut(s) 837, 994, 1096, 1198
MseI TTAA 3 cut(s) 524, 705, 843
MslI CAYNNNNRTG 1 cut(s) 681
MspA1I CMGCKG 1 cut(s) 567
MspI CCGG 3 cut(s) 531, 972, 1237
MunI CAATTG 1 cut(s) 909
Mva1269I GAATGC 1 cut(s) 570
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 4 cut(s) 192, 555, 564, 873
NdeI CATATG 1 cut(s) 182
NdeII GATC 5 cut(s) 354, 376, 403, 485, 772
NlaIII CATG 2 cut(s) 874, 956
NlaIV GGNNCC 6 cut(s) 101, 123, 505, 825, 895, 979
NspV TTCGAA 2 cut(s) 337, 388
PaeR7I CTCGAG 1 cut(s) 1048
PceI AGGCCT 3 cut(s) 343, 436, 949
PciSI GCTCTTC 1 cut(s) 927
PctI GAATGC 1 cut(s) 570
PfeI GAWTC 6 cut(s) 220, 673, 731, 782, 905, 1122
PinAI ACCGGT 1 cut(s) 1236
PkrI GCNGC 9 cut(s) 188, 271, 316, 551, 566, 569, 757, 866, 934
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
PspN4I GGNNCC 6 cut(s) 101, 123, 505, 825, 895, 979
PspPI GGNCC 2 cut(s) 1204, 1239
PspXI VCTCGAGB 1 cut(s) 1048
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 1 cut(s) 812
RsaNI GTAC 1 cut(s) 811
RseI CAYNNNNRTG 1 cut(s) 681
SapI GCTCTTC 1 cut(s) 927
SaqAI TTAA 3 cut(s) 524, 705, 843
SatI GCNGC 9 cut(s) 187, 270, 315, 550, 565, 568, 756, 865, 933
Sau3AI GATC 5 cut(s) 354, 376, 403, 485, 772
Sau96I GGNCC 2 cut(s) 1204, 1239
SchI GAGTC 1 cut(s) 81
SduI GDGCHC 1 cut(s) 608
SfaNI GCATC 5 cut(s) 301, 451, 610, 652, 1189
SfcI CTRYAG 1 cut(s) 666
Sfr274I CTCGAG 1 cut(s) 1048
SfuI TTCGAA 2 cut(s) 337, 388
SinI GGWCC 1 cut(s) 1239
SlaI CTCGAG 1 cut(s) 1048
SmiMI CAYNNNNRTG 1 cut(s) 681
SmlI CTYRAG 1 cut(s) 1048
SmoI CTYRAG 1 cut(s) 1048
Sse9I AATT 6 cut(s) 33, 398, 497, 844, 909, 965
SseBI AGGCCT 3 cut(s) 343, 436, 949
SsiI CCGC 5 cut(s) 186, 293, 565, 865, 876
SspMI CTAG 3 cut(s) 230, 752, 1208
StuI AGGCCT 3 cut(s) 343, 436, 949
StyI CCWWGG 2 cut(s) 67, 344
TaaI ACNGT 5 cut(s) 115, 266, 428, 790, 994
TaiI ACGT 1 cut(s) 207
TaqI TCGA 5 cut(s) 198, 337, 388, 889, 1049
TasI AATT 6 cut(s) 33, 398, 497, 844, 909, 965
TatI WGTACW 1 cut(s) 810
TauI GCSGC 3 cut(s) 189, 567, 867
TfiI GAWTC 6 cut(s) 220, 673, 731, 782, 905, 1122
Tru1I TTAA 3 cut(s) 524, 705, 843
Tru9I TTAA 3 cut(s) 524, 705, 843
TscAI CASTG 3 cut(s) 12, 288, 364
TseI GCWGC 6 cut(s) 269, 314, 549, 567, 755, 932
TspDTI ATGAA 3 cut(s) 665, 815, 1071
TspRI CASTG 3 cut(s) 12, 288, 364
VpaK11BI GGWCC 1 cut(s) 1239
XagI CCTNNNNNAGG 1 cut(s) 89
XapI RAATTY 1 cut(s) 398
XhoI CTCGAG 1 cut(s) 1048
XspI CTAG 3 cut(s) 230, 752, 1208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.