Rh4BG264400

Iron-Sulfur binding protein C terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014717
Physical Location & Seq
Forward (+)
43827013 .. 43829730
2718 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG264400.1

Sequence Viewer

Length: 1254 bp
ATGGCACTGAGCTTCTTCTCCTGTTGTGCTTCAATTGCTCCTCAACAACATCAAGCAAAACTGAACCTTGGAGTCAACAAAGCCCTTGAAGAGGTAAGGAGCCTCATAAAAACTGTTGGGGTTCCATCAGTTGCGTCCTCTCCAGTAAAATCACTTCAAAATGGCAACTGGGTCAAGCTCATATGCGGCGCGAGCTTCGAGGATGTAGTTGATATAAGGAATCTCTCGCTAGTTTACACTCTTGCAGGAGTGGATTGCATAGACTGTGCAGCAGATGTGTCGGTGGTGAGCGCGGTAAATGAGGGGATTGATGCAGCATTGGAGATTTTCCCTCTTCCAAGGCCTTGGGTGATGATCAGTGTGAATGATGATGAGGATCTTCACTTTCGAAAAGCTGAATTTGATCCAGAGGATTGTCCACTTGACTGTTCAAGGCCTTGTGAGAGAGTATGTCCTGCTGATGCAATATCCTTTGAGGCTGGAAGATCAATTACACAATTCTCTTATGCCACCAAAACACCTGCCTTTTTAAAGGCCGGAGTTCTAACTGAGCGCTGCTATGGCTGTGGCCGCTGCATTCCAGTCTGCCCATACGATAAAATAAGTGTGAGCACATACATAAGAGATGCTACTGCAACCTCTGAACTTCTCAAAAGAAATGATGTTGATGCTATAGAGATTCATACAAGTGGAAGGCAGATTGCTCCATTTAAAGAACTTTGGGATGGTTTGGGAGATTCAATCAAACATCTCAGACTAGCAGCAGTTAGTTTACCTGATCTTGGAGAGTCAACCGTATCAACAATGAAGGCTATGTACTCAATTATGGAACCGGATTTATGTTGCTTCAATTTATGGCAGTTGGATGGCCGCCCCATGAGCGGAGATATTGGTCGAGGTGCCACAAGAGAATCAATTGCTTTTGCTCTTCGTTTGGCTGCTATAAAAGAAAGGCCTCATGGTTTTCTTCAATTAGCGGGTGGCACCAATGCTCACACAGTTGATGGGTTGAGGAAAGTCGGACTTTTTCAAGCGGCATCCTTGCCAAAGAACCCGAGGGATGAAAACTCGACAGCTATGATTGGTGGTGTTGCTTATGGTGGCTATGCACGAAAGATTGTTGGAAGAATCCTGAGTTCTATGCAATCACAACACGGCATTACTGGGATTGAGGATTATCCAGAGCATCTCCTTCAAGCACTTGGAGAGGCCTTAGCCTTAGTTGGAAAACTCAAATGTTACCGGTCCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4 Fer4_9 LdpA_C

Protein Analysis

417

Amino Acids

45.12

Weight (kDa)

5.82

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LdpA_Fe-S-bd PF25160 139 - 206 8.7e-21 LdpA Fe-S cluster-binding domain
Fer4_9 PF13187 142 - 199 1.6e-06 4Fe-4S dicluster domain
Fer4_7 PF12838 143 - 199 4e-06 4Fe-4S dicluster domain
LdpA_C PF12617 216 - 409 8.9e-63 Light dependent period A C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014717)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g19730 FvH4_4g19730 FvH4_4g19730 FvH4_4g19730 FvH4_4g19730
malus_domestica MD16G1154200.v1.1
prunus_persica Prupe.1G117500_v2.0.a1 Prupe.1G117500_v2.0.a1
pyrus_communis pycom16g13060
rosa_chinensis RchiOBHm_Chr4g0425231
rosa_laevigata RLG00000007384
rosa_multiflora Rmu_sc0004716.1_g000005
rosa_roxburghii Rroxscaffold_5G00367260
rosa_rugosa Rorug04G0202000
rosa_samantha Rh4BG264400 Rh4DG260500
rosa_wichuraiana Rw4G022410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 529
Acc36I ACCTGC 1 cut(s) 529
AccB1I GGYRCC 2 cut(s) 899, 983
AccBSI CCGCTC 1 cut(s) 882
AccII CGCG 2 cut(s) 191, 293
AciI CCGC 7 cut(s) 186, 293, 571, 871, 882, 977, 1034
AclWI GGATC 2 cut(s) 384, 398
AcoI YGGCCR 2 cut(s) 568, 868
AcsI RAATTY 1 cut(s) 398
AfaI GTAC 1 cut(s) 818
AfeI AGCGCT 1 cut(s) 554
AfiI CCNNNNNNNGG 3 cut(s) 91, 782, 881
AgeI ACCGGT 1 cut(s) 1242
AgsI TTSAA 9 cut(s) 33, 89, 158, 432, 741, 850, 971, 1031, 1196
AluBI AGCT 5 cut(s) 12, 178, 195, 395, 1076
AluI AGCT 5 cut(s) 12, 178, 195, 395, 1076
Alw21I GWGCWC 1 cut(s) 614
AlwI GGATC 2 cut(s) 384, 398
Ama87I CYCGRG 1 cut(s) 1054
Aor51HI AGCGCT 1 cut(s) 554
AoxI GGCC 7 cut(s) 341, 434, 534, 568, 868, 953, 1209
ApeKI GCWGC 6 cut(s) 269, 314, 555, 573, 761, 938
ApoI RAATTY 1 cut(s) 398
AsiGI ACCGGT 1 cut(s) 1242
AspLEI GCGC 3 cut(s) 191, 293, 555
AspS9I GGNCC 1 cut(s) 1245
AsuHPI GGTGA 2 cut(s) 298, 361
AsuII TTCGAA 1 cut(s) 388
AvaI CYCGRG 1 cut(s) 1054
AvaII GGWCC 1 cut(s) 1245
BanI GGYRCC 2 cut(s) 899, 983
BarI GAAGNNNNNNTAC 6 cut(s) 138, 170, 475, 507, 800, 832
Bbv12I GWGCWC 1 cut(s) 614
BbvI GCAGC 6 cut(s) 281, 326, 542, 560, 773, 925
BccI CCATC 4 cut(s) 133, 719, 860, 998
BceAI ACGGC 1 cut(s) 1171
BcgI CGANNNNNNTGC 2 cut(s) 261, 295
BclI TGATCA 1 cut(s) 354
BfaI CTAG 2 cut(s) 230, 758
BfmI CTRYAG 1 cut(s) 672
BfoI RGCGCY 1 cut(s) 556
BfuAI ACCTGC 1 cut(s) 529
Bme18I GGWCC 1 cut(s) 1245
BmeT110I CYCGRG 1 cut(s) 1054
BmgT120I GGNCC 1 cut(s) 1245
BmiI GGNNCC 5 cut(s) 101, 123, 831, 901, 985
BmrI ACTGGG 2 cut(s) 178, 1173
BmsI GCATC 6 cut(s) 301, 451, 616, 658, 1046, 1195
BmuI ACTGGG 2 cut(s) 178, 1173
BpmI CTGGAG 1 cut(s) 126
Bpu10I CCTNAGC 1 cut(s) 1213
Bpu14I TTCGAA 1 cut(s) 388
BsaBI GATNNNNATC 1 cut(s) 375
BsaJI CCNNGG 4 cut(s) 67, 338, 344, 1055
BsaWI WCCGGW 2 cut(s) 832, 1242
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 782, 881
Bse118I RCCGGY 1 cut(s) 1242
Bse1I ACTGG 4 cut(s) 143, 173, 581, 1168
Bse8I GATNNNNATC 1 cut(s) 375
BseDI CCNNGG 4 cut(s) 67, 338, 344, 1055
BseGI GGATG 5 cut(s) 208, 730, 871, 1037, 1066
BseJI GATNNNNATC 1 cut(s) 375
BseLI CCNNNNNNNGG 3 cut(s) 91, 782, 881
BseMII CTCAG 3 cut(s) 540, 766, 1124
BseNI ACTGG 4 cut(s) 143, 173, 581, 1168
BseRI GAGGAG 1 cut(s) 30
BseXI GCAGC 6 cut(s) 281, 326, 542, 560, 773, 925
BsgI GTGCAG 1 cut(s) 288
Bsh1236I CGCG 2 cut(s) 191, 293
BshFI GGCC 7 cut(s) 343, 436, 536, 570, 870, 955, 1211
BshNI GGYRCC 2 cut(s) 899, 983
BshTI ACCGGT 1 cut(s) 1242
BsiHKAI GWGCWC 1 cut(s) 614
BsiHKCI CYCGRG 1 cut(s) 1054
BsiSI CCGG 3 cut(s) 537, 833, 1243
BslI CCNNNNNNNGG 3 cut(s) 91, 782, 881
BsmI GAATGC 1 cut(s) 576
BsnI GGCC 7 cut(s) 343, 436, 536, 570, 870, 955, 1211
BsoBI CYCGRG 1 cut(s) 1054
Bsp119I TTCGAA 1 cut(s) 388
Bsp1286I GDGCHC 1 cut(s) 614
Bsp143I GATC 5 cut(s) 354, 376, 403, 485, 778
BspACI CCGC 7 cut(s) 186, 293, 571, 871, 882, 977, 1034
BspANI GGCC 7 cut(s) 343, 436, 536, 570, 870, 955, 1211
BspCNI CTCAG 3 cut(s) 541, 765, 1125
BspFNI CGCG 2 cut(s) 191, 293
BspLI GGNNCC 5 cut(s) 101, 123, 831, 901, 985
BspMI ACCTGC 1 cut(s) 529
BspPI GGATC 2 cut(s) 384, 398
BspQI GCTCTTC 1 cut(s) 933
BspT104I TTCGAA 1 cut(s) 388
BspT107I GGYRCC 2 cut(s) 899, 983
BsrBI CCGCTC 1 cut(s) 882
BsrFI RCCGGY 1 cut(s) 1242
BsrI ACTGG 4 cut(s) 143, 173, 581, 1168
BssAI RCCGGY 1 cut(s) 1242
BssECI CCNNGG 4 cut(s) 67, 338, 344, 1055
BssMI GATC 5 cut(s) 354, 376, 403, 485, 778
BssT1I CCWWGG 3 cut(s) 67, 338, 344
Bst4CI ACNGT 5 cut(s) 115, 266, 428, 796, 1000
Bst6I CTCTTC 3 cut(s) 84, 339, 933
BstBI TTCGAA 1 cut(s) 388
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 6 cut(s) 8, 549, 752, 1133, 1213, 1219
BstENI CCTNNNNNAGG 1 cut(s) 89
BstF5I GGATG 5 cut(s) 208, 730, 871, 1037, 1066
BstFNI CGCG 2 cut(s) 191, 293
BstH2I RGCGCY 1 cut(s) 556
BstHHI GCGC 3 cut(s) 191, 293, 555
BstKTI GATC 5 cut(s) 357, 379, 406, 488, 781
BstMBI GATC 5 cut(s) 354, 376, 403, 485, 778
BstMWI GCNNNNNNNGC 5 cut(s) 35, 192, 561, 570, 879
BstSFI CTRYAG 1 cut(s) 672
BstUI CGCG 2 cut(s) 191, 293
BstV1I GCAGC 6 cut(s) 281, 326, 542, 560, 773, 925
BstX2I RGATCY 1 cut(s) 376
BstXI CCANNNNNNTGG 1 cut(s) 345
BstYI RGATCY 1 cut(s) 376
BsuRI GGCC 7 cut(s) 343, 436, 536, 570, 870, 955, 1211
BtsCI GGATG 5 cut(s) 208, 730, 871, 1037, 1066
BtsIMutI CAGTG 2 cut(s) 5, 364
BveI ACCTGC 1 cut(s) 529
Cac8I GCNNGC 1 cut(s) 193
CfoI GCGC 3 cut(s) 191, 293, 555
Cfr10I RCCGGY 1 cut(s) 1242
Cfr13I GGNCC 1 cut(s) 1245
CseI GACGC 1 cut(s) 123
Csp6I GTAC 1 cut(s) 817
CspAI ACCGGT 1 cut(s) 1242
CviAII CATG 2 cut(s) 877, 959
CviQI GTAC 1 cut(s) 817
DdeI CTNAG 6 cut(s) 8, 549, 752, 1133, 1213, 1219
DpnI GATC 5 cut(s) 356, 378, 405, 487, 780
DpnII GATC 5 cut(s) 354, 376, 403, 485, 778
DraI TTTAAA 2 cut(s) 531, 712
EaeI YGGCCR 2 cut(s) 568, 868
Eam1104I CTCTTC 3 cut(s) 84, 339, 933
EarI CTCTTC 3 cut(s) 84, 339, 933
Eco130I CCWWGG 3 cut(s) 67, 338, 344
Eco147I AGGCCT 4 cut(s) 343, 436, 955, 1211
Eco47I GGWCC 1 cut(s) 1245
Eco47III AGCGCT 1 cut(s) 554
Eco88I CYCGRG 1 cut(s) 1054
EcoNI CCTNNNNNAGG 1 cut(s) 89
EcoT14I CCWWGG 3 cut(s) 67, 338, 344
ErhI CCWWGG 3 cut(s) 67, 338, 344
FaeI CATG 2 cut(s) 880, 962
FalI AAGNNNNNCTT 2 cut(s) 1008, 1040
FatI CATG 2 cut(s) 876, 958
FauI CCCGC 1 cut(s) 970
FauNDI CATATG 1 cut(s) 182
FbaI TGATCA 1 cut(s) 354
FokI GGATG 5 cut(s) 215, 737, 878, 1024, 1073
FspBI CTAG 2 cut(s) 230, 758
GlaI GCGC 3 cut(s) 190, 292, 554
GsuI CTGGAG 1 cut(s) 126
HaeII RGCGCY 1 cut(s) 556
HaeIII GGCC 7 cut(s) 343, 436, 536, 570, 870, 955, 1211
HapII CCGG 3 cut(s) 537, 833, 1243
HgaI GACGC 1 cut(s) 123
HhaI GCGC 3 cut(s) 191, 293, 555
Hin1II CATG 2 cut(s) 880, 962
Hin6I GCGC 3 cut(s) 189, 291, 553
HinP1I GCGC 3 cut(s) 189, 291, 553
HincII GTYRAC 2 cut(s) 76, 792
HindII GTYRAC 2 cut(s) 76, 792
HinfI GANTC 7 cut(s) 72, 220, 679, 737, 788, 911, 1128
HpaII CCGG 3 cut(s) 537, 833, 1243
HphI GGTGA 2 cut(s) 298, 361
Hpy166II GTNNAC 5 cut(s) 76, 235, 419, 773, 792
Hpy188I TCNGA 3 cut(s) 643, 755, 1022
Hpy188III TCNNGA 3 cut(s) 407, 1132, 1181
Hpy8I GTNNAC 5 cut(s) 76, 235, 419, 773, 792
HpyAV CCTTC 3 cut(s) 687, 802, 1202
HpyCH4III ACNGT 5 cut(s) 115, 266, 428, 796, 1000
HpyCH4V TGCA 9 cut(s) 245, 258, 269, 314, 464, 576, 635, 1109, 1144
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 192, 561, 570, 879
HpyF3I CTNAG 6 cut(s) 8, 549, 752, 1133, 1213, 1219
Hsp92II CATG 2 cut(s) 880, 962
HspAI GCGC 3 cut(s) 189, 291, 553
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 5 cut(s) 354, 376, 403, 485, 778
LguI GCTCTTC 1 cut(s) 933
LmnI GCTCC 3 cut(s) 43, 99, 709
Lsp1109I GCAGC 6 cut(s) 281, 326, 542, 560, 773, 925
LweI GCATC 6 cut(s) 301, 451, 616, 658, 1046, 1195
MaeI CTAG 2 cut(s) 230, 758
MaeIII GTNAC 1 cut(s) 1238
MalI GATC 5 cut(s) 356, 378, 405, 487, 780
MbiI CCGCTC 1 cut(s) 882
MboI GATC 5 cut(s) 354, 376, 403, 485, 778
MboII GAAGA 8 cut(s) 7, 101, 326, 371, 495, 920, 959, 1137
MfeI CAATTG 2 cut(s) 33, 915
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 1 cut(s) 614
MluCI AATT 8 cut(s) 33, 398, 489, 497, 822, 850, 915, 971
MlyI GAGTC 2 cut(s) 81, 797
MmeI TCCRAC 4 cut(s) 843, 1000, 1102, 1204
MseI TTAA 2 cut(s) 530, 711
MslI CAYNNNNRTG 1 cut(s) 687
MspA1I CMGCKG 1 cut(s) 573
MspI CCGG 3 cut(s) 537, 833, 1243
MunI CAATTG 2 cut(s) 33, 915
Mva1269I GAATGC 1 cut(s) 576
MvnI CGCG 2 cut(s) 191, 293
MwoI GCNNNNNNNGC 5 cut(s) 35, 192, 561, 570, 879
NdeI CATATG 1 cut(s) 182
NdeII GATC 5 cut(s) 354, 376, 403, 485, 778
NlaIII CATG 2 cut(s) 880, 962
NlaIV GGNNCC 5 cut(s) 101, 123, 831, 901, 985
NspV TTCGAA 1 cut(s) 388
PaqCI CACCTGC 1 cut(s) 529
PceI AGGCCT 4 cut(s) 343, 436, 955, 1211
PciSI GCTCTTC 1 cut(s) 933
PctI GAATGC 1 cut(s) 576
PfeI GAWTC 5 cut(s) 220, 679, 737, 911, 1128
PinAI ACCGGT 1 cut(s) 1242
PleI GAGTC 2 cut(s) 80, 796
PpsI GAGTC 2 cut(s) 80, 796
PspN4I GGNNCC 5 cut(s) 101, 123, 831, 901, 985
PspPI GGNCC 1 cut(s) 1245
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 1 cut(s) 818
RsaNI GTAC 1 cut(s) 817
RseI CAYNNNNRTG 1 cut(s) 687
SapI GCTCTTC 1 cut(s) 933
SaqAI TTAA 2 cut(s) 530, 711
Sau3AI GATC 5 cut(s) 354, 376, 403, 485, 778
Sau96I GGNCC 1 cut(s) 1245
SchI GAGTC 2 cut(s) 81, 797
SduI GDGCHC 1 cut(s) 614
SfaNI GCATC 6 cut(s) 301, 451, 616, 658, 1046, 1195
SfcI CTRYAG 1 cut(s) 672
SfuI TTCGAA 1 cut(s) 388
SinI GGWCC 1 cut(s) 1245
SmiMI CAYNNNNRTG 1 cut(s) 687
Sse9I AATT 8 cut(s) 33, 398, 489, 497, 822, 850, 915, 971
SseBI AGGCCT 4 cut(s) 343, 436, 955, 1211
SsiI CCGC 7 cut(s) 186, 293, 571, 871, 882, 977, 1034
SspMI CTAG 2 cut(s) 230, 758
StuI AGGCCT 4 cut(s) 343, 436, 955, 1211
StyI CCWWGG 3 cut(s) 67, 338, 344
TaaI ACNGT 5 cut(s) 115, 266, 428, 796, 1000
TaqI TCGA 4 cut(s) 198, 388, 895, 1070
TasI AATT 8 cut(s) 33, 398, 489, 497, 822, 850, 915, 971
TatI WGTACW 1 cut(s) 816
TauI GCSGC 4 cut(s) 189, 573, 873, 1037
TfiI GAWTC 5 cut(s) 220, 679, 737, 911, 1128
Tru1I TTAA 2 cut(s) 530, 711
Tru9I TTAA 2 cut(s) 530, 711
TscAI CASTG 2 cut(s) 12, 364
TseI GCWGC 6 cut(s) 269, 314, 555, 573, 761, 938
TspDTI ATGAA 3 cut(s) 671, 821, 1077
TspRI CASTG 2 cut(s) 12, 364
VpaK11BI GGWCC 1 cut(s) 1245
XagI CCTNNNNNAGG 1 cut(s) 89
XapI RAATTY 1 cut(s) 398
XspI CTAG 2 cut(s) 230, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.