MD16G1154200.v1.1

Iron-Sulfur binding protein C terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014717
Physical Location & Seq
Reverse (-)
12239627 .. 12242583
2957 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1154200.v1.1.491

Sequence Viewer

Length: 1269 bp
ATGATGGCTTTGAGCTTCCTTTGCAGTGCTTCATTTTCTCCTCACCACCAACACCAAGCCAAAGTGAAGGTTACAAGCAGCAAAGGCATTGAAGAAGTGAAGAATCTCATAAAAACTGTTGGGATTCCATCAAAAACCTCCTCTCCAGCAAAATCTCTTGAAAATGGCAACTGGGTCAAGCTCATCTGCGGCGCAAGCTTCGAGGATGCGGTTGATATCAGGAATCTTTCTCTGGTTTACACTCTTGCAGGAGTTGATTGCATAGACTGTGCGGCGGATGTGTCAGTGGTGAGTGCAGTGAACGAGGGGATTGATGCTGCAATTGGGATTTGTCCCCTTCCGAGGCCTTGGGTGATGATCAGCGTTAATGATGACGAAGATCTTCACTTTCGCAAGGCCGAGTTTGATCCAGAGGATTGTCCACTTGACTGTTCACGGCCTTGTGAGAGAGTTTGTCCTGCTGATGCAATATCATTGGGGGCTCAAAAATCAACAGGACAGTTCTTCTATGGAACCAATTTACCTGAAACATTTAAGGGTGGAGTTCTAACTGAACGCTGCTATGGCTGCGGCCGCTGCATTCCAGTATGCCCCTACGATAAAATAATGTCCTCGTACGTAAGAGATGCTTATGCAACCTCCGAACTTCTCAGAAGAAATGATGTTGATGCATTAGAGATTCATACAACTGGAAGGCAGATTGCTCCATTTAAAGAACTTTGGGATGGCTTGGGTGATTCGGTTAAGTGTCTGCGACTAGCAGCAGTTAGCTTGCCTGATAATGGGGAATCAACTGTATCGTCGATGAATGCTATGTACTCAGTTATGGAACCAGATCTGTGTTGCTTCAATCTATGGCAGTTGGATGGCCGCCCCATGAGTGGAGATATTGGCCGAGGTGCCACAAGAGAATCGATTGCTTTTGCTCTTCGTTTGGCTGCTATAAAAGAAAGACCTTGTGGATTTCTTCAATTGGCGGGTGGCACCAATGCTCACACCGTTGATGGGTTGAGGAAAGTCGGACTTTTTCAAACGGCATCCTTCCCAAATACAACTGGATCACCTAATTCACTGCATCCAATGATTAGTGGTGTTGCTTATGGTGGCTATGCACGAAAGATTGTTGGAAGAATCCTGAGCTATATGCAATCACAGCATGGCATCGCCAGTATTGAGGTTTATCCAGAGCATCTCCTTAAGGCACTCAGAGAGGCACTTTCTTTGGTAGGAACTGTCAAATGTTACAATCCGTTTCCAGCAAATACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4 Fer4_9 LdpA_C

Protein Analysis

423

Amino Acids

45.65

Weight (kDa)

6.02

Isoelectric Point (pI)

34.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LdpA_Fe-S-bd PF25160 140 - 203 7.7e-21 LdpA Fe-S cluster-binding domain
Fer4_9 PF13187 143 - 200 6e-06 4Fe-4S dicluster domain
Fer4_7 PF12838 146 - 200 4.2e-06 4Fe-4S dicluster domain
LdpA_C PF12617 216 - 410 5.3e-61 Light dependent period A C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014717)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g19730 FvH4_4g19730 FvH4_4g19730 FvH4_4g19730 FvH4_4g19730
malus_domestica MD16G1154200.v1.1
prunus_persica Prupe.1G117500_v2.0.a1 Prupe.1G117500_v2.0.a1
pyrus_communis pycom16g13060
rosa_chinensis RchiOBHm_Chr4g0425231
rosa_laevigata RLG00000007384
rosa_multiflora Rmu_sc0004716.1_g000005
rosa_roxburghii Rroxscaffold_5G00367260
rosa_rugosa Rorug04G0202000
rosa_samantha Rh4BG264400 Rh4DG260500
rosa_wichuraiana Rw4G022410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 899, 983
AciI CCGC 8 cut(s) 189, 209, 272, 275, 570, 574, 871, 977
AclWI GGATC 2 cut(s) 401, 1066
AcoI YGGCCR 3 cut(s) 571, 868, 892
AfaI GTAC 2 cut(s) 617, 818
AfiI CCNNNNNNNGG 4 cut(s) 342, 782, 881, 1005
AflII CTTAAG 1 cut(s) 1196
AgsI TTSAA 5 cut(s) 92, 161, 850, 971, 1031
AluBI AGCT 5 cut(s) 15, 181, 198, 771, 1140
AluI AGCT 5 cut(s) 15, 181, 198, 771, 1140
AlwI GGATC 2 cut(s) 401, 1066
AoxI GGCC 6 cut(s) 344, 396, 437, 571, 868, 892
ApeKI GCWGC 7 cut(s) 78, 317, 558, 567, 576, 761, 938
Asp700I GAANNNNTTC 1 cut(s) 381
AspLEI GCGC 1 cut(s) 194
AsuHPI GGTGA 5 cut(s) 35, 301, 364, 746, 1053
BanI GGYRCC 2 cut(s) 899, 983
BanII GRGCYC 1 cut(s) 484
BbvI GCAGC 7 cut(s) 90, 304, 545, 554, 563, 773, 925
BccI CCATC 4 cut(s) 136, 719, 860, 998
BceAI ACGGC 2 cut(s) 452, 1050
BclI TGATCA 1 cut(s) 357
BfaI CTAG 1 cut(s) 758
BfrI CTTAAG 1 cut(s) 1196
BglII AGATCT 2 cut(s) 379, 835
BmiI GGNNCC 4 cut(s) 514, 831, 901, 985
BmrI ACTGGG 1 cut(s) 181
BmsI GCATC 9 cut(s) 196, 304, 454, 616, 658, 1046, 1084, 1170, 1198
BmuI ACTGGG 1 cut(s) 181
BpmI CTGGAG 1 cut(s) 129
Bpu10I CCTNAGC 1 cut(s) 1136
Bsa29I ATCGAT 1 cut(s) 914
BsaAI YACGTR 1 cut(s) 619
BsaJI CCNNGG 3 cut(s) 341, 347, 895
BsaXI ACNNNNNCTCC 2 cut(s) 127, 157
Bsc4I CCNNNNNNNGG 4 cut(s) 342, 782, 881, 1005
Bse1I ACTGG 5 cut(s) 176, 584, 694, 1060, 1167
BseCI ATCGAT 1 cut(s) 914
BseDI CCNNGG 3 cut(s) 341, 347, 895
BseGI GGATG 6 cut(s) 211, 283, 730, 871, 1037, 1075
BseLI CCNNNNNNNGG 4 cut(s) 342, 782, 881, 1005
BseMII CTCAG 4 cut(s) 664, 834, 1127, 1219
BseNI ACTGG 5 cut(s) 176, 584, 694, 1060, 1167
BseRI GAGGAG 2 cut(s) 30, 130
BseX3I CGGCCG 1 cut(s) 571
BseXI GCAGC 7 cut(s) 90, 304, 545, 554, 563, 773, 925
BsgI GTGCAG 1 cut(s) 315
Bsh1285I CGRYCG 1 cut(s) 574
BshFI GGCC 6 cut(s) 346, 398, 439, 573, 870, 894
BshNI GGYRCC 2 cut(s) 899, 983
BshVI ATCGAT 1 cut(s) 914
BsiEI CGRYCG 1 cut(s) 574
BsiWI CGTACG 1 cut(s) 615
BslFI GGGAC 1 cut(s) 318
BslI CCNNNNNNNGG 4 cut(s) 342, 782, 881, 1005
BsmFI GGGAC 1 cut(s) 318
BsmI GAATGC 2 cut(s) 579, 814
BsnI GGCC 6 cut(s) 346, 398, 439, 573, 870, 894
Bsp1286I GDGCHC 1 cut(s) 484
Bsp143I GATC 5 cut(s) 357, 379, 406, 835, 1058
BspACI CCGC 8 cut(s) 189, 209, 272, 275, 570, 574, 871, 977
BspANI GGCC 6 cut(s) 346, 398, 439, 573, 870, 894
BspCNI CTCAG 4 cut(s) 663, 833, 1128, 1218
BspDI ATCGAT 1 cut(s) 914
BspLI GGNNCC 4 cut(s) 514, 831, 901, 985
BspPI GGATC 2 cut(s) 401, 1066
BspQI GCTCTTC 1 cut(s) 933
BspT107I GGYRCC 2 cut(s) 899, 983
BspTI CTTAAG 1 cut(s) 1196
BsrI ACTGG 5 cut(s) 176, 584, 694, 1060, 1167
BssECI CCNNGG 3 cut(s) 341, 347, 895
BssMI GATC 5 cut(s) 357, 379, 406, 835, 1058
BssT1I CCWWGG 1 cut(s) 347
Bst4CI ACNGT 7 cut(s) 118, 269, 431, 501, 796, 1000, 1234
Bst6I CTCTTC 1 cut(s) 933
BstAFI CTTAAG 1 cut(s) 1196
BstBAI YACGTR 1 cut(s) 619
BstC8I GCNNGC 2 cut(s) 196, 773
BstDEI CTNAG 4 cut(s) 650, 820, 1136, 1205
BstF5I GGATG 6 cut(s) 211, 283, 730, 871, 1037, 1075
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 5 cut(s) 360, 382, 409, 838, 1061
BstMBI GATC 5 cut(s) 357, 379, 406, 835, 1058
BstMCI CGRYCG 1 cut(s) 574
BstMWI GCNNNNNNNGC 8 cut(s) 21, 84, 195, 564, 567, 573, 576, 1153
BstSNI TACGTA 1 cut(s) 619
BstV1I GCAGC 7 cut(s) 90, 304, 545, 554, 563, 773, 925
BstX2I RGATCY 2 cut(s) 379, 835
BstYI RGATCY 2 cut(s) 379, 835
BstZI CGGCCG 1 cut(s) 571
Bsu15I ATCGAT 1 cut(s) 914
BsuRI GGCC 6 cut(s) 346, 398, 439, 573, 870, 894
BsuTUI ATCGAT 1 cut(s) 914
BtgZI GCGATG 1 cut(s) 1147
BtsCI GGATG 6 cut(s) 211, 283, 730, 871, 1037, 1075
BtsI GCAGTG 3 cut(s) 31, 303, 1070
BtsIMutI CAGTG 4 cut(s) 31, 291, 303, 1070
Cac8I GCNNGC 2 cut(s) 196, 773
CciNI GCGGCCGC 1 cut(s) 571
CfoI GCGC 1 cut(s) 194
ClaI ATCGAT 1 cut(s) 914
Csp6I GTAC 2 cut(s) 616, 817
CviAII CATG 2 cut(s) 877, 1157
CviQI GTAC 2 cut(s) 616, 817
DdeI CTNAG 4 cut(s) 650, 820, 1136, 1205
DpnI GATC 5 cut(s) 359, 381, 408, 837, 1060
DpnII GATC 5 cut(s) 357, 379, 406, 835, 1058
DraI TTTAAA 1 cut(s) 712
EaeI YGGCCR 3 cut(s) 571, 868, 892
EagI CGGCCG 1 cut(s) 571
Eam1104I CTCTTC 1 cut(s) 933
EarI CTCTTC 1 cut(s) 933
EciI GGCGGA 1 cut(s) 290
EclXI CGGCCG 1 cut(s) 571
Eco105I TACGTA 1 cut(s) 619
Eco130I CCWWGG 1 cut(s) 347
Eco147I AGGCCT 1 cut(s) 346
Eco24I GRGCYC 1 cut(s) 484
Eco32I GATATC 1 cut(s) 217
Eco52I CGGCCG 1 cut(s) 571
EcoRV GATATC 1 cut(s) 217
EcoT14I CCWWGG 1 cut(s) 347
EcoT22I ATGCAT 1 cut(s) 673
EcoT38I GRGCYC 1 cut(s) 484
ErhI CCWWGG 1 cut(s) 347
FaeI CATG 2 cut(s) 880, 1160
FalI AAGNNNNNCTT 4 cut(s) 613, 645, 1008, 1040
FaqI GGGAC 1 cut(s) 318
FatI CATG 2 cut(s) 876, 1156
FauI CCCGC 1 cut(s) 970
FbaI TGATCA 1 cut(s) 357
FokI GGATG 6 cut(s) 218, 290, 737, 878, 1024, 1062
FriOI GRGCYC 1 cut(s) 484
FspBI CTAG 1 cut(s) 758
GlaI GCGC 1 cut(s) 193
GsuI CTGGAG 1 cut(s) 129
HaeIII GGCC 6 cut(s) 346, 398, 439, 573, 870, 894
HhaI GCGC 1 cut(s) 194
Hin1II CATG 2 cut(s) 880, 1160
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HindIII AAGCTT 1 cut(s) 196
HinfI GANTC 8 cut(s) 103, 124, 223, 679, 737, 788, 911, 1131
HphI GGTGA 5 cut(s) 35, 301, 364, 746, 1053
Hpy166II GTNNAC 4 cut(s) 238, 301, 422, 434
Hpy188I TCNGA 5 cut(s) 342, 643, 653, 1022, 1208
Hpy188III TCNNGA 5 cut(s) 158, 220, 410, 1135, 1184
Hpy8I GTNNAC 4 cut(s) 238, 301, 422, 434
Hpy99I CGWCG 1 cut(s) 805
HpyAV CCTTC 4 cut(s) 61, 347, 687, 1051
HpyCH4III ACNGT 7 cut(s) 118, 269, 431, 501, 796, 1000, 1234
HpyCH4IV ACGT 1 cut(s) 618
HpyF10VI GCNNNNNNNGC 8 cut(s) 21, 84, 195, 564, 567, 573, 576, 1153
HpyF3I CTNAG 4 cut(s) 650, 820, 1136, 1205
HpySE526I ACGT 1 cut(s) 618
Hsp92II CATG 2 cut(s) 880, 1160
HspAI GCGC 1 cut(s) 192
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 5 cut(s) 357, 379, 406, 835, 1058
LguI GCTCTTC 1 cut(s) 933
LmnI GCTCC 1 cut(s) 709
Lsp1109I GCAGC 7 cut(s) 90, 304, 545, 554, 563, 773, 925
LweI GCATC 9 cut(s) 196, 304, 454, 616, 658, 1046, 1084, 1170, 1198
MaeI CTAG 1 cut(s) 758
MaeII ACGT 1 cut(s) 618
MaeIII GTNAC 2 cut(s) 70, 1241
MalI GATC 5 cut(s) 359, 381, 408, 837, 1060
MboI GATC 5 cut(s) 357, 379, 406, 835, 1058
MboII GAAGA 9 cut(s) 104, 112, 374, 389, 496, 666, 920, 959, 1140
MfeI CAATTG 2 cut(s) 321, 971
MflI RGATCY 2 cut(s) 379, 835
MhlI GDGCHC 1 cut(s) 484
MluCI AATT 4 cut(s) 321, 517, 971, 1066
MmeI TCCRAC 3 cut(s) 843, 1000, 1105
Mph1103I ATGCAT 1 cut(s) 673
MroXI GAANNNNTTC 1 cut(s) 381
MseI TTAA 6 cut(s) 366, 534, 711, 744, 1197, 1267
MspA1I CMGCKG 1 cut(s) 576
MspCI CTTAAG 1 cut(s) 1196
MunI CAATTG 2 cut(s) 321, 971
Mva1269I GAATGC 2 cut(s) 579, 814
MwoI GCNNNNNNNGC 8 cut(s) 21, 84, 195, 564, 567, 573, 576, 1153
NdeII GATC 5 cut(s) 357, 379, 406, 835, 1058
NlaIII CATG 2 cut(s) 880, 1160
NlaIV GGNNCC 4 cut(s) 514, 831, 901, 985
NmeAIII GCCGAG 2 cut(s) 424, 920
NotI GCGGCCGC 1 cut(s) 571
NsiI ATGCAT 1 cut(s) 673
PceI AGGCCT 1 cut(s) 346
PciSI GCTCTTC 1 cut(s) 933
PctI GAATGC 2 cut(s) 579, 814
PdmI GAANNNNTTC 1 cut(s) 381
PfeI GAWTC 8 cut(s) 103, 124, 223, 679, 737, 788, 911, 1131
Pfl23II CGTACG 1 cut(s) 615
Ppu21I YACGTR 1 cut(s) 619
PspLI CGTACG 1 cut(s) 615
PspN4I GGNNCC 4 cut(s) 514, 831, 901, 985
PsuI RGATCY 2 cut(s) 379, 835
RsaI GTAC 2 cut(s) 617, 818
RsaNI GTAC 2 cut(s) 616, 817
SapI GCTCTTC 1 cut(s) 933
SaqAI TTAA 6 cut(s) 366, 534, 711, 744, 1197, 1267
Sau3AI GATC 5 cut(s) 357, 379, 406, 835, 1058
SduI GDGCHC 1 cut(s) 484
SfaNI GCATC 9 cut(s) 196, 304, 454, 616, 658, 1046, 1084, 1170, 1198
SmlI CTYRAG 1 cut(s) 1196
SmoI CTYRAG 1 cut(s) 1196
SnaBI TACGTA 1 cut(s) 619
Sse9I AATT 4 cut(s) 321, 517, 971, 1066
SseBI AGGCCT 1 cut(s) 346
SsiI CCGC 8 cut(s) 189, 209, 272, 275, 570, 574, 871, 977
SspMI CTAG 1 cut(s) 758
StuI AGGCCT 1 cut(s) 346
StyI CCWWGG 1 cut(s) 347
TaaI ACNGT 7 cut(s) 118, 269, 431, 501, 796, 1000, 1234
TaiI ACGT 1 cut(s) 621
TaqI TCGA 3 cut(s) 201, 803, 914
TasI AATT 4 cut(s) 321, 517, 971, 1066
TatI WGTACW 1 cut(s) 816
TauI GCSGC 5 cut(s) 192, 275, 573, 576, 873
TfiI GAWTC 8 cut(s) 103, 124, 223, 679, 737, 788, 911, 1131
Tru1I TTAA 6 cut(s) 366, 534, 711, 744, 1197, 1267
Tru9I TTAA 6 cut(s) 366, 534, 711, 744, 1197, 1267
TscAI CASTG 4 cut(s) 31, 291, 303, 1077
TseI GCWGC 7 cut(s) 78, 317, 558, 567, 576, 761, 938
TspDTI ATGAA 3 cut(s) 21, 671, 821
TspGWI ACGGA 1 cut(s) 1239
TspRI CASTG 4 cut(s) 31, 291, 303, 1077
Vha464I CTTAAG 1 cut(s) 1196
XmnI GAANNNNTTC 1 cut(s) 381
XspI CTAG 1 cut(s) 758
Zsp2I ATGCAT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.