FvH4_4g20990

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008992
Physical Location & Seq
Forward (+)
24160644 .. 24166058
5415 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20990.t1

Sequence Viewer

Length: 1464 bp
ATGGAGCACCGTACAGTAATAATCCTTTGGATTTCAATCATCCTTGTTCTAATCCAGAATCCAAGCAATGTACAAGGAAGATATCACTACCATAAAAAGAGCAGTAAAGGGTCACAATCCCCTACTCCATCCCCTGAATCCCCTCAATCTCCAGAGCCGGTTGCTCCCCCAAGTACTCCGGTAGCTCCTTCAACTCCGGTGGTTCCTTCTACACCAAGCATTCCTCCGAATGTTCCGTCGGACCCTTACCCGGGGGACGACCCCACAGATTGTATATTCAACGTCATGTCGTACGGAGCCGTCGGAGATGGTGATGCTGATGACACCAAGGCGTTCAGGGATGCTTGGAAGGCTGCATGCGCGGTTGAATCCGCTGTCGTTTTGGCTCCGGCGGATTACTGCTTCAAGATCACTTCAACTATTTTTTCAGGTCCTTGCAAGCCTGGACTAGTATTCCAAGTCGATGGGATTTTGATGACACCAGACGGGCCAAAGGCTTGGCCAAAAGTAGACAGCAATAAGCAATGGCTTGTATTTTATCGACTTGACCAAATGACGTTCACTGGAAGTGGAACCATCGAAGGGAATGGCCAAAAATGGTGGGATCTGCCTTGCAAGCCTCACAGGGGTCCCAATGGATCCACAGCATCAGGACCATGTGATAGCCCTGCTTTGATTCGATTCTTCATGAGCTCAAATCTGGTGGTGAAAGGGTTAAGAATCCAAAACAGCCCTATGTTCCACATGAAATTCGACGGCTGCGAAGGAGTCCATATCGAAAGGCTGTCGATTTCGTCCCCGAAACTTAGCCCTAACACAGATGGAATCCACATAGAGAACACAAAATCTGTAGGAATTTATGACTCGATGATAAGCAATGGGGATGACTGCATTTCAATAGGTCCAGGATGTGCCAACGTTGATATAATGGGTGTCACTTGCGGTCCAAGTCACGGAATTAGCATTGGGAGCCTTGGTGTCCACAACTCGCAGGCATGTGTTTCAAACATCACAGTCCGCAATGTCGTCATAAGGGAATCGGATAACGGCCTCCGAATCAAGACATGGCAAGGCGGAACGGGCTGCGTCTCGGGAATACTTTTCGAAAACATCAACATGGAGAATGTGATGAACTGCTTGCTCGTCGACCAGTACTACTGCACCTCCAAGGGTTGCTTGAACCAAACCTCGGCCGTCTACGTCACCGACCTGACCTATCGAATGATCAAGGGCACATATGATGTGAGAAAGCCTCCAATTCACTTTGCTTGCAGCGACACTGTGGCTTGCACCAACATTACACTCTCAGAAGTTGAGCTTTACCCATATGAAGGTGAGCTAATGGATGACCCTTTTTGTTGGAATGCCTATGGGACTCAAGAGACTCCAACTATTCCCCCTATTGAGTGCTTACAAGATGGTCAGCCTGAGGCTTTGGCAGAGGTGGCTCAGTATACTTGCTAG

Protein Analysis

488

Amino Acids

52.72

Weight (kDa)

5.02

Isoelectric Point (pI)

50.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 127 - 440 3.3e-83 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 4 cut(s) 510, 1146, 1197, 1454
AccII CGCG 1 cut(s) 362
AciI CCGC 6 cut(s) 362, 372, 392, 942, 1018, 1074
AclI AACGTT 1 cut(s) 918
AclWI GGATC 3 cut(s) 612, 633, 646
AcoI YGGCCR 3 cut(s) 500, 589, 1191
AcsI RAATTY 2 cut(s) 749, 855
AfaI GTAC 5 cut(s) 13, 72, 175, 293, 1154
AfiI CCNNNNNNNGG 7 cut(s) 250, 251, 582, 626, 953, 1189, 1331
AgsI TTSAA 9 cut(s) 36, 192, 280, 368, 406, 417, 897, 1005, 1180
AhlI ACTAGT 1 cut(s) 448
AjnI CCWGG 2 cut(s) 442, 904
AluBI AGCT 4 cut(s) 185, 693, 1318, 1339
AluI AGCT 4 cut(s) 185, 693, 1318, 1339
Alw21I GWGCWC 2 cut(s) 9, 695
Alw26I GTCTC 2 cut(s) 1093, 1376
AlwI GGATC 3 cut(s) 612, 633, 646
Ama87I CYCGRG 2 cut(s) 250, 1090
AoxI GGCC 5 cut(s) 488, 500, 589, 1048, 1191
ApeKI GCWGC 4 cut(s) 353, 759, 1083, 1272
ApoI RAATTY 2 cut(s) 749, 855
ArsI GACNNNNNNTTYG 2 cut(s) 221, 253
AspLEI GCGC 1 cut(s) 362
AspS9I GGNCC 7 cut(s) 241, 431, 488, 629, 653, 902, 944
AsuC2I CCSGG 2 cut(s) 251, 252
AsuHPI GGTGA 4 cut(s) 323, 718, 1195, 1346
AsuII TTCGAA 1 cut(s) 1104
AvaI CYCGRG 2 cut(s) 250, 1090
AvaII GGWCC 6 cut(s) 241, 431, 629, 653, 902, 944
AxyI CCTNAGG 1 cut(s) 1428
BaeGI GKGCMC 1 cut(s) 1235
BalI TGGCCA 2 cut(s) 502, 591
BamHI GGATCC 1 cut(s) 638
BanII GRGCYC 1 cut(s) 695
Bbv12I GWGCWC 2 cut(s) 9, 695
BbvI GCAGC 4 cut(s) 340, 746, 1070, 1284
BccI CCATC 6 cut(s) 136, 302, 458, 584, 815, 1412
BceAI ACGGC 4 cut(s) 284, 772, 1063, 1178
BciT130I CCWGG 2 cut(s) 444, 906
BclI TGATCA 1 cut(s) 1224
BcnI CCSGG 2 cut(s) 251, 252
BcoDI GTCTC 2 cut(s) 1093, 1376
BcuI ACTAGT 1 cut(s) 448
BfaI CTAG 2 cut(s) 449, 1462
BfmI CTRYAG 1 cut(s) 849
BisI GCNGC 4 cut(s) 354, 760, 1084, 1273
BlsI GCNGC 4 cut(s) 355, 761, 1085, 1274
BmcAI AGTACT 2 cut(s) 175, 1154
Bme1390I CCNGG 4 cut(s) 251, 252, 444, 906
Bme18I GGWCC 6 cut(s) 241, 431, 629, 653, 902, 944
BmeT110I CYCGRG 2 cut(s) 250, 1090
BmgT120I GGNCC 7 cut(s) 241, 431, 488, 629, 653, 902, 944
BmiI GGNNCC 9 cut(s) 204, 243, 298, 387, 574, 630, 631, 640, 971
BmrFI CCNGG 4 cut(s) 251, 252, 444, 906
BmsI GCATC 3 cut(s) 304, 331, 656
BplI GAGNNNNNCTC 2 cut(s) 1237, 1269
BpmI CTGGAG 1 cut(s) 135
Bpu14I TTCGAA 1 cut(s) 1104
BpuEI CTTGAG 1 cut(s) 1362
BpuMI CCSGG 2 cut(s) 251, 252
BsaBI GATNNNNATC 1 cut(s) 35
BsaJI CCNNGG 6 cut(s) 250, 251, 327, 973, 1167, 1188
BsaWI WCCGGW 2 cut(s) 178, 196
BsaXI ACNNNNNCTCC 4 cut(s) 961, 991, 1148, 1178
Bsc4I CCNNNNNNNGG 7 cut(s) 250, 251, 582, 626, 953, 1189, 1331
Bse118I RCCGGY 1 cut(s) 157
Bse1I ACTGG 2 cut(s) 568, 1150
Bse21I CCTNAGG 1 cut(s) 1428
Bse3DI GCAATG 4 cut(s) 73, 530, 883, 1027
Bse8I GATNNNNATC 1 cut(s) 35
BseBI CCWGG 2 cut(s) 444, 906
BseDI CCNNGG 6 cut(s) 250, 251, 327, 973, 1167, 1188
BseGI GGATG 6 cut(s) 39, 128, 346, 889, 914, 1351
BseJI GATNNNNATC 1 cut(s) 35
BseLI CCNNNNNNNGG 7 cut(s) 250, 251, 582, 626, 953, 1189, 1331
BseMI GCAATG 4 cut(s) 73, 530, 883, 1027
BseMII CTCAG 3 cut(s) 1320, 1419, 1463
BseNI ACTGG 2 cut(s) 568, 1150
BseSI GKGCMC 1 cut(s) 1235
BseX3I CGGCCG 1 cut(s) 1191
BseXI GCAGC 4 cut(s) 340, 746, 1070, 1284
BsgI GTGCAG 1 cut(s) 1144
Bsh1236I CGCG 1 cut(s) 362
Bsh1285I CGRYCG 1 cut(s) 1194
BshFI GGCC 5 cut(s) 490, 502, 591, 1050, 1193
BsiEI CGRYCG 1 cut(s) 1194
BsiHKAI GWGCWC 2 cut(s) 9, 695
BsiHKCI CYCGRG 2 cut(s) 250, 1090
BsiSI CCGG 5 cut(s) 158, 179, 197, 251, 389
BsiWI CGTACG 1 cut(s) 291
BslFI GGGAC 4 cut(s) 269, 615, 781, 1387
BslI CCNNNNNNNGG 7 cut(s) 250, 251, 582, 626, 953, 1189, 1331
BsmAI GTCTC 2 cut(s) 1093, 1376
BsmBI CGTCTC 1 cut(s) 1093
BsmFI GGGAC 4 cut(s) 269, 615, 781, 1387
BsmI GAATGC 2 cut(s) 219, 1369
BsnI GGCC 5 cut(s) 490, 502, 591, 1050, 1193
BsoBI CYCGRG 2 cut(s) 250, 1090
Bsp119I TTCGAA 1 cut(s) 1104
Bsp1286I GDGCHC 3 cut(s) 9, 695, 1235
Bsp1407I TGTACA 1 cut(s) 70
Bsp143I GATC 4 cut(s) 408, 604, 638, 1224
BspACI CCGC 6 cut(s) 362, 372, 392, 942, 1018, 1074
BspANI GGCC 5 cut(s) 490, 502, 591, 1050, 1193
BspCNI CTCAG 3 cut(s) 1319, 1420, 1462
BspFNI CGCG 1 cut(s) 362
BspHI TCATGA 1 cut(s) 687
BspLI GGNNCC 9 cut(s) 204, 243, 298, 387, 574, 630, 631, 640, 971
BspPI GGATC 3 cut(s) 612, 633, 646
BspT104I TTCGAA 1 cut(s) 1104
BsrDI GCAATG 4 cut(s) 73, 530, 883, 1027
BsrFI RCCGGY 1 cut(s) 157
BsrGI TGTACA 1 cut(s) 70
BsrI ACTGG 2 cut(s) 568, 1150
BssAI RCCGGY 1 cut(s) 157
BssECI CCNNGG 6 cut(s) 250, 251, 327, 973, 1167, 1188
BssMI GATC 4 cut(s) 408, 604, 638, 1224
BssNAI GTATAC 1 cut(s) 1455
BssT1I CCWWGG 3 cut(s) 327, 973, 1167
Bst1107I GTATAC 1 cut(s) 1455
Bst2UI CCWGG 2 cut(s) 444, 906
Bst4CI ACNGT 4 cut(s) 11, 16, 1015, 1282
BstAUI TGTACA 1 cut(s) 70
BstBI TTCGAA 1 cut(s) 1104
BstC8I GCNNGC 7 cut(s) 358, 440, 617, 993, 1139, 1270, 1288
BstDEI CTNAG 4 cut(s) 806, 1306, 1428, 1449
BstF5I GGATG 6 cut(s) 39, 128, 346, 889, 914, 1351
BstFNI CGCG 1 cut(s) 362
BstHHI GCGC 1 cut(s) 362
BstKTI GATC 4 cut(s) 411, 607, 641, 1227
BstMAI GTCTC 2 cut(s) 1093, 1376
BstMBI GATC 4 cut(s) 408, 604, 638, 1224
BstMCI CGRYCG 1 cut(s) 1194
BstMWI GCNNNNNNNGC 6 cut(s) 350, 359, 616, 969, 1080, 1445
BstNI CCWGG 2 cut(s) 444, 906
BstNSI RCATGY 2 cut(s) 360, 999
BstSCI CCNGG 4 cut(s) 249, 250, 442, 904
BstSFI CTRYAG 1 cut(s) 849
BstSLI GKGCMC 1 cut(s) 1235
BstUI CGCG 1 cut(s) 362
BstV1I GCAGC 4 cut(s) 340, 746, 1070, 1284
BstX2I RGATCY 2 cut(s) 604, 638
BstXI CCANNNNNNTGG 2 cut(s) 464, 498
BstYI RGATCY 2 cut(s) 604, 638
BstZ17I GTATAC 1 cut(s) 1455
BstZI CGGCCG 1 cut(s) 1191
Bsu36I CCTNAGG 1 cut(s) 1428
BsuRI GGCC 5 cut(s) 490, 502, 591, 1050, 1193
BtsCI GGATG 6 cut(s) 39, 128, 346, 889, 914, 1351
BtsIMutI CAGTG 2 cut(s) 561, 1278
Cac8I GCNNGC 7 cut(s) 358, 440, 617, 993, 1139, 1270, 1288
CciI TCATGA 1 cut(s) 687
CfoI GCGC 1 cut(s) 362
Cfr10I RCCGGY 1 cut(s) 157
Cfr13I GGNCC 7 cut(s) 241, 431, 488, 629, 653, 902, 944
Cfr9I CCCGGG 1 cut(s) 250
CseI GACGC 1 cut(s) 1075
Csp6I GTAC 5 cut(s) 12, 71, 174, 292, 1153
CspCI CAANNNNNGTGG 6 cut(s) 180, 215, 581, 616, 684, 719
CviAII CATG 8 cut(s) 286, 357, 657, 688, 745, 996, 1065, 1117
CviQI GTAC 5 cut(s) 12, 71, 174, 292, 1153
DdeI CTNAG 4 cut(s) 806, 1306, 1428, 1449
DpnI GATC 4 cut(s) 410, 606, 640, 1226
DpnII GATC 4 cut(s) 408, 604, 638, 1224
EaeI YGGCCR 3 cut(s) 500, 589, 1191
EagI CGGCCG 1 cut(s) 1191
EciI GGCGGA 2 cut(s) 407, 1089
Ecl136II GAGCTC 1 cut(s) 693
EclXI CGGCCG 1 cut(s) 1191
Eco130I CCWWGG 3 cut(s) 327, 973, 1167
Eco24I GRGCYC 1 cut(s) 695
Eco32I GATATC 1 cut(s) 83
Eco47I GGWCC 6 cut(s) 241, 431, 629, 653, 902, 944
Eco52I CGGCCG 1 cut(s) 1191
Eco53kI GAGCTC 1 cut(s) 693
Eco81I CCTNAGG 1 cut(s) 1428
Eco88I CYCGRG 2 cut(s) 250, 1090
EcoICRI GAGCTC 1 cut(s) 693
EcoO109I RGGNCCY 2 cut(s) 431, 629
EcoRII CCWGG 2 cut(s) 442, 904
EcoRV GATATC 1 cut(s) 83
EcoT14I CCWWGG 3 cut(s) 327, 973, 1167
EcoT38I GRGCYC 1 cut(s) 695
ErhI CCWWGG 3 cut(s) 327, 973, 1167
Esp3I CGTCTC 1 cut(s) 1093
FaeI CATG 8 cut(s) 289, 360, 660, 691, 748, 999, 1068, 1120
FalI AAGNNNNNCTT 4 cut(s) 1160, 1192, 1302, 1334
FaqI GGGAC 4 cut(s) 269, 615, 781, 1387
FatI CATG 8 cut(s) 285, 356, 656, 687, 744, 995, 1064, 1116
FauNDI CATATG 2 cut(s) 1237, 1327
FbaI TGATCA 1 cut(s) 1224
FblI GTMKAC 4 cut(s) 510, 1146, 1197, 1454
Fnu4HI GCNGC 4 cut(s) 354, 760, 1084, 1273
FokI GGATG 6 cut(s) 26, 115, 353, 896, 921, 1358
FriOI GRGCYC 1 cut(s) 695
Fsp4HI GCNGC 4 cut(s) 354, 760, 1084, 1273
FspBI CTAG 2 cut(s) 449, 1462
GlaI GCGC 1 cut(s) 361
GluI GCNGC 4 cut(s) 354, 760, 1084, 1273
GsuI CTGGAG 1 cut(s) 135
HaeIII GGCC 5 cut(s) 490, 502, 591, 1050, 1193
HapII CCGG 5 cut(s) 158, 179, 197, 251, 389
HgaI GACGC 1 cut(s) 1075
HhaI GCGC 1 cut(s) 362
Hin1II CATG 8 cut(s) 289, 360, 660, 691, 748, 999, 1068, 1120
Hin6I GCGC 1 cut(s) 360
HinP1I GCGC 1 cut(s) 360
HincII GTYRAC 1 cut(s) 1147
HindII GTYRAC 1 cut(s) 1147
HpaII CCGG 5 cut(s) 158, 179, 197, 251, 389
HphI GGTGA 4 cut(s) 323, 718, 1195, 1346
Hpy166II GTNNAC 6 cut(s) 511, 561, 982, 1147, 1198, 1455
Hpy188I TCNGA 6 cut(s) 228, 241, 305, 1042, 1055, 1309
Hpy188III TCNNGA 8 cut(s) 55, 152, 406, 651, 688, 1060, 1092, 1379
Hpy8I GTNNAC 6 cut(s) 511, 561, 982, 1147, 1198, 1455
Hpy99I CGWCG 4 cut(s) 241, 305, 758, 1148
HpyAV CCTTC 6 cut(s) 198, 216, 343, 575, 758, 1325
HpyCH4III ACNGT 4 cut(s) 11, 16, 1015, 1282
HpyCH4IV ACGT 4 cut(s) 282, 557, 918, 1200
HpyCH4V TGCA 7 cut(s) 356, 438, 615, 891, 1161, 1272, 1290
HpyF10VI GCNNNNNNNGC 6 cut(s) 350, 359, 616, 969, 1080, 1445
HpyF3I CTNAG 4 cut(s) 806, 1306, 1428, 1449
HpySE526I ACGT 4 cut(s) 282, 557, 918, 1200
Hsp92II CATG 8 cut(s) 289, 360, 660, 691, 748, 999, 1068, 1120
HspAI GCGC 1 cut(s) 360
KflI GGGWCCC 1 cut(s) 629
Ksp22I TGATCA 1 cut(s) 1224
Kzo9I GATC 4 cut(s) 408, 604, 638, 1224
LmnI GCTCC 6 cut(s) 4, 169, 190, 296, 391, 969
Lsp1109I GCAGC 4 cut(s) 340, 746, 1070, 1284
LweI GCATC 3 cut(s) 304, 331, 656
MaeI CTAG 2 cut(s) 449, 1462
MaeII ACGT 4 cut(s) 282, 557, 918, 1200
MaeIII GTNAC 4 cut(s) 111, 934, 950, 1201
MalI GATC 4 cut(s) 410, 606, 640, 1226
MboI GATC 4 cut(s) 408, 604, 638, 1224
MboII GAAGA 2 cut(s) 90, 676
MflI RGATCY 2 cut(s) 604, 638
MhlI GDGCHC 3 cut(s) 9, 695, 1235
MlsI TGGCCA 2 cut(s) 502, 591
MluCI AATT 4 cut(s) 749, 855, 957, 1257
MluNI TGGCCA 2 cut(s) 502, 591
MlyI GAGTC 4 cut(s) 777, 857, 1369, 1378
MmeI TCCRAC 4 cut(s) 219, 283, 1340, 1412
MnlI CCTC 9 cut(s) 153, 234, 630, 1061, 1174, 1198, 1263, 1423, 1435
Mox20I TGGCCA 2 cut(s) 502, 591
MscI TGGCCA 2 cut(s) 502, 591
MseI TTAA 1 cut(s) 716
MslI CAYNNNNRTG 1 cut(s) 1115
Msp20I TGGCCA 2 cut(s) 502, 591
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 5 cut(s) 158, 179, 197, 251, 389
MspR9I CCNGG 4 cut(s) 251, 252, 444, 906
Mva1269I GAATGC 2 cut(s) 219, 1369
MvaI CCWGG 2 cut(s) 444, 906
MvnI CGCG 1 cut(s) 362
MwoI GCNNNNNNNGC 6 cut(s) 350, 359, 616, 969, 1080, 1445
NciI CCSGG 2 cut(s) 251, 252
NdeI CATATG 2 cut(s) 1237, 1327
NdeII GATC 4 cut(s) 408, 604, 638, 1224
NlaIII CATG 8 cut(s) 289, 360, 660, 691, 748, 999, 1068, 1120
NlaIV GGNNCC 9 cut(s) 204, 243, 298, 387, 574, 630, 631, 640, 971
NmeAIII GCCGAG 1 cut(s) 1169
NmuCI GTSAC 4 cut(s) 111, 934, 950, 1201
NspI RCATGY 2 cut(s) 360, 999
NspV TTCGAA 1 cut(s) 1104
PaeI GCATGC 1 cut(s) 360
PagI TCATGA 1 cut(s) 687
PcsI WCGNNNNNNNCGW 1 cut(s) 759
PctI GAATGC 2 cut(s) 219, 1369
PfeI GAWTC 9 cut(s) 58, 137, 368, 676, 681, 720, 825, 1037, 1056
Pfl23II CGTACG 1 cut(s) 291
PfoI TCCNGGA 1 cut(s) 904
PkrI GCNGC 4 cut(s) 355, 761, 1085, 1274
PleI GAGTC 4 cut(s) 776, 857, 1369, 1378
PpsI GAGTC 4 cut(s) 776, 857, 1369, 1378
PpuMI RGGWCCY 2 cut(s) 431, 629
Psp124BI GAGCTC 1 cut(s) 695
Psp1406I AACGTT 1 cut(s) 918
Psp5II RGGWCCY 2 cut(s) 431, 629
Psp6I CCWGG 2 cut(s) 442, 904
PspGI CCWGG 2 cut(s) 442, 904
PspLI CGTACG 1 cut(s) 291
PspN4I GGNNCC 9 cut(s) 204, 243, 298, 387, 574, 630, 631, 640, 971
PspPI GGNCC 7 cut(s) 241, 431, 488, 629, 653, 902, 944
PspPPI RGGWCCY 2 cut(s) 431, 629
PsuI RGATCY 2 cut(s) 604, 638
RsaI GTAC 5 cut(s) 13, 72, 175, 293, 1154
RsaNI GTAC 5 cut(s) 12, 71, 174, 292, 1153
RseI CAYNNNNRTG 1 cut(s) 1115
SacI GAGCTC 1 cut(s) 695
SalI GTCGAC 1 cut(s) 1145
SaqAI TTAA 1 cut(s) 716
SatI GCNGC 4 cut(s) 354, 760, 1084, 1273
Sau3AI GATC 4 cut(s) 408, 604, 638, 1224
Sau96I GGNCC 7 cut(s) 241, 431, 488, 629, 653, 902, 944
ScaI AGTACT 2 cut(s) 175, 1154
SchI GAGTC 4 cut(s) 777, 857, 1369, 1378
ScrFI CCNGG 4 cut(s) 251, 252, 444, 906
SduI GDGCHC 3 cut(s) 9, 695, 1235
SfaNI GCATC 3 cut(s) 304, 331, 656
SfcI CTRYAG 1 cut(s) 849
SfuI TTCGAA 1 cut(s) 1104
SinI GGWCC 6 cut(s) 241, 431, 629, 653, 902, 944
SmaI CCCGGG 1 cut(s) 252
SmiMI CAYNNNNRTG 1 cut(s) 1115
SmlI CTYRAG 1 cut(s) 1377
SmoI CTYRAG 1 cut(s) 1377
SpeI ACTAGT 1 cut(s) 448
SphI GCATGC 1 cut(s) 360
Sse9I AATT 4 cut(s) 749, 855, 957, 1257
SsiI CCGC 6 cut(s) 362, 372, 392, 942, 1018, 1074
SspMI CTAG 2 cut(s) 449, 1462
SstI GAGCTC 1 cut(s) 695
StyD4I CCNGG 4 cut(s) 249, 250, 442, 904
StyI CCWWGG 3 cut(s) 327, 973, 1167
TaaI ACNGT 4 cut(s) 11, 16, 1015, 1282
TaiI ACGT 4 cut(s) 285, 560, 921, 1203
TasI AATT 4 cut(s) 749, 855, 957, 1257
TatI WGTACW 3 cut(s) 70, 173, 1152
TfiI GAWTC 9 cut(s) 58, 137, 368, 676, 681, 720, 825, 1037, 1056
Tru1I TTAA 1 cut(s) 716
Tru9I TTAA 1 cut(s) 716
TscAI CASTG 2 cut(s) 568, 1285
TseFI GTSAC 4 cut(s) 111, 934, 950, 1201
TseI GCWGC 4 cut(s) 353, 759, 1083, 1272
Tsp45I GTSAC 4 cut(s) 111, 934, 950, 1201
TspDTI ATGAA 4 cut(s) 676, 761, 1145, 1344
TspGWI ACGGA 3 cut(s) 225, 309, 969
TspMI CCCGGG 1 cut(s) 250
TspRI CASTG 2 cut(s) 568, 1285
VpaK11BI GGWCC 6 cut(s) 241, 431, 629, 653, 902, 944
XapI RAATTY 2 cut(s) 749, 855
XceI RCATGY 2 cut(s) 360, 999
XmaI CCCGGG 1 cut(s) 250
XmiI GTMKAC 4 cut(s) 510, 1146, 1197, 1454
XspI CTAG 2 cut(s) 449, 1462
ZrmI AGTACT 2 cut(s) 175, 1154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.