Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008992

Physical Location & Seq

Genomic Coordinates Reverse (-)

10800275 .. 10806093

Gene Structure 5819 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1140500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1443 bp

ATGGAGCTCTTTCACTGGTTTAGACTCTTATGGATCGCTATGACTCTATTGACCTTCCGGTGTGCAAGCAATGTGGAAGGAAGATATCATCACCAAAAGAGGAACAAAAACTCATCCCCTCCTACAACCCCTGCAGAAGATCCAGTTGCTCCTTCAACTCCGAGCATTGCTCCTCCTACCCCTCCTCGAGATGTTCCCTCAGACCCTTACCCAACTGACCCCGGAAACAACTTTAGTAATTCTTCAGACTGTGTGTTCAACGTCATGGATTATGGGGCAGTTGGAGATGGCTCTGCTGATGACACTGCTGCATTTAGACAGGCTTGGAAAGAAGCCTGTGCTGTTAAATCAGGTGTTGTTTTCGCTCCTTCAGATTACCGCTTCAAAATCACCTCAACAATTTTTTCAGGTCCATGTCAGCCAGGACTAGTATTCCAAGTAGATGGGGTTTTGATGCCGCCGGACGGGCCAGCGACGTGGCCTAAAAAAGACAGCAAACAACAGTGGCTTGTGTTTTACAAACTTAACCGGATGACTTTCACTGGTACTGGAACCATTGAAGGTAACGGCCAAAAGTGGTGGGCCCTCCCTTGCAAACCTCACAGGGGTCCCAATGGATCAACACTGCGAGGACCATGTGACAGCCCTGCTATGATCAGATTTTTCATGAGCTCTAATTTGGTGGTGAGAGGCTTAAGAATCCAGAACAGTCCCCAATTCCACATGAAATTCGATGGCTGTGTAGGAGTACTGATTCAGAAAATATCCATTTCTTCACCAAAGCTTAGCCCCAACACAGATGGGATCCACATAGAGAACACAAAATCTGTTGCCATACACAACTCAGTGATTAGCAACGGTGATGACTGCATCTCGATAGGAACTGGGTGTGCAAATGTTGATATAATGGGTGTCACTTGTGGGCCAAGTCACGGGATTAGCATTGGGAGCCTTGGCGTGCACAACTCCCAGGCATGCGTCTCCAACATCACAGTCCGCAACTCCGTCATACGTGAATCCGACAACGGTCTCCGAATCAAGACATGGCAAGGCGGGACGGGCAGCGTCTCTGGCATCCTCTTCGAGAACATCCAGATGGAGAATGTGCTCAACTGCCTGCTAGTAGACCAGTACTACTGCCTATCACAAGCATGCCGAAACGAAACCTCAGCAGTACTAGTCACAGACTTGACCTACAGAAACATAAAGGGCACGTACGATGTGAGGAGGCCTCCCATTCACTTTGCGTGCAGTGACACCGTGGCATGCAGAAATATTATCCTCTCAGAAGTTGAGCTTTACCCATACGAAGGAGAGCTCATGGATGATCCCTTTTGTTGGAATGCTTATGGGACACAGGAGACTGCAACTATTCCCCCTATTGATTGCTTGCGAGAGGGGGAACCTCAGGCTTTGGACGAGGTGTCCAAGTATACTTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004650 GO:0005575 GO:0005618 GO:0005623 GO:0008150 GO:0009664 GO:0009825 GO:0009827 GO:0009831 GO:0009987 GO:0016043 GO:0016049 GO:0016787 GO:0016798 GO:0030312 GO:0040007 GO:0042545 GO:0042547 GO:0044464 GO:0045229 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

Glyco_hydro_28

Protein Analysis

481

Amino Acids

52.6

Weight (kDa)

5.95

Isoelectric Point (pI)

42.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pect-lyase_RHGA_epim	PF12708	86 - 129	6.4e-06	Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28	PF00295	122 - 433	9.8e-83	Glycosyl hydrolases family 28

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008992)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48100
fragaria_vesca	FvH4_4g20990 FvH4_4g20990
malus_domestica	MD13G1146600.v1.1 MD16G1140500.v1.1
prunus_persica	Prupe.1G110100_v2.0.a1 Prupe.1G110100_v2.0.a1 Prupe.1G110100_v2.0.a1
pyrus_communis	pycom13g12630 pycom16g12470
rosa_chinensis	RchiOBHm_Chr4g0426531
rosa_laevigata	RLG00000007292
rosa_roxburghii	Rroxscaffold_5G00368540
rosa_rugosa	Rorug04G0212700 Rorug04G0212700 Rorug04G0212700 Rorug04G0212700
rosa_samantha	Rh4AG268200 Rh4BG274500 Rh4CG288900 Rh4DG271300
rosa_wichuraiana	Rw4G023270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	1125, 1433
AciI	CCGC	4 cut(s)	379, 458, 997, 1053
AclWI	GGATC	6 cut(s)	41, 134, 625, 799, 812, 1322
AcoI	YGGCCR	1 cut(s)	568
AcsI	RAATTY	1 cut(s)	728
AcuI	CTGAAG	2 cut(s)	228, 354
AfaI	GTAC	5 cut(s)	547, 750, 1133, 1176, 1217
AfiI	CCNNNNNNNGG	5 cut(s)	464, 605, 932, 1310, 1338
AflII	CTTAAG	1 cut(s)	694
AgsI	TTSAA	4 cut(s)	156, 259, 385, 560
AhdI	GACNNNNNGTC	1 cut(s)	1423
AhlI	ACTAGT	2 cut(s)	427, 1177
AjiI	CACGTC	1 cut(s)	477
AjnI	CCWGG	2 cut(s)	421, 969
AluBI	AGCT	5 cut(s)	7, 672, 784, 1297, 1318
AluI	AGCT	5 cut(s)	7, 672, 784, 1297, 1318
Alw21I	GWGCWC	5 cut(s)	9, 674, 963, 1110, 1320
Alw26I	GTCTC	4 cut(s)	985, 1034, 1072, 1355
Alw44I	GTGCAC	1 cut(s)	959
AlwI	GGATC	6 cut(s)	41, 134, 625, 799, 812, 1322
Ama87I	CYCGRG	1 cut(s)	186
AoxI	GGCC	6 cut(s)	467, 479, 568, 582, 923, 1229
ApaI	GGGCCC	1 cut(s)	586
ApaLI	GTGCAC	1 cut(s)	959
ApeKI	GCWGC	2 cut(s)	308, 1062
ApoI	RAATTY	1 cut(s)	728
AspS9I	GGNCC	7 cut(s)	410, 467, 582, 583, 608, 632, 923
AsuC2I	CCSGG	1 cut(s)	222
AsuHPI	GGTGA	5 cut(s)	83, 382, 697, 768, 872
AvaI	CYCGRG	1 cut(s)	186
AvaII	GGWCC	3 cut(s)	410, 608, 632
AxyI	CCTNAGG	1 cut(s)	1407
BaeGI	GKGCMC	3 cut(s)	586, 963, 1214
BamHI	GGATCC	1 cut(s)	804
BanII	GRGCYC	4 cut(s)	9, 586, 674, 1320
Bbv12I	GWGCWC	5 cut(s)	9, 674, 963, 1110, 1320
BbvCI	CCTCAGC	1 cut(s)	1168
BbvI	GCAGC	2 cut(s)	295, 1074
BccI	CCATC	5 cut(s)	281, 437, 728, 794, 1090
BceAI	ACGGC	1 cut(s)	583
BcgI	CGANNNNNNTGC	2 cut(s)	1063, 1097
BciT130I	CCWGG	2 cut(s)	423, 971
BclI	TGATCA	1 cut(s)	654
BcnI	CCSGG	1 cut(s)	222
BcoDI	GTCTC	4 cut(s)	985, 1034, 1072, 1355
BcuI	ACTAGT	2 cut(s)	427, 1177
BfaI	CTAG	3 cut(s)	428, 1121, 1178
BfmI	CTRYAG	2 cut(s)	132, 1195
BfrI	CTTAAG	1 cut(s)	694
BglI	GCCNNNNNGGC	1 cut(s)	466
BisI	GCNGC	3 cut(s)	309, 458, 1063
BlpI	GCTNAGC	1 cut(s)	785
BlsI	GCNGC	3 cut(s)	310, 459, 1064
BmcAI	AGTACT	3 cut(s)	750, 1133, 1176
Bme1390I	CCNGG	3 cut(s)	222, 423, 971
Bme18I	GGWCC	3 cut(s)	410, 608, 632
BmeRI	GACNNNNNGTC	1 cut(s)	1423
BmeT110I	CYCGRG	1 cut(s)	186
BmgBI	CACGTC	1 cut(s)	477
BmgT120I	GGNCC	7 cut(s)	410, 467, 582, 583, 608, 632, 923
BmiI	GGNNCC	7 cut(s)	553, 584, 609, 610, 806, 950, 1404
BmrFI	CCNGG	3 cut(s)	222, 423, 971
BmrI	ACTGGG	1 cut(s)	894
BmsI	GCATC	3 cut(s)	444, 879, 1083
BmuI	ACTGGG	1 cut(s)	894
BoxI	GACNNNNGTC	1 cut(s)	1026
BplI	GAGNNNNNCTC	4 cut(s)	154, 186, 1216, 1248
Bpu10I	CCTNAGC	1 cut(s)	1168
Bpu1102I	GCTNAGC	1 cut(s)	785
BpuMI	CCSGG	1 cut(s)	222
BsaAI	YACGTR	2 cut(s)	1013, 1215
BsaI	GGTCTC	1 cut(s)	1034
BsaJI	CCNNGG	4 cut(s)	220, 952, 969, 1260
BsaWI	WCCGGW	2 cut(s)	57, 528
Bsc4I	CCNNNNNNNGG	5 cut(s)	464, 605, 932, 1310, 1338
Bse1I	ACTGG	6 cut(s)	20, 143, 547, 553, 889, 1129
Bse21I	CCTNAGG	1 cut(s)	1407
Bse3DI	GCAATG	2 cut(s)	76, 165
BseBI	CCWGG	2 cut(s)	423, 971
BseDI	CCNNGG	4 cut(s)	220, 952, 969, 1260
BseGI	GGATG	5 cut(s)	113, 537, 1074, 1089, 1330
BseLI	CCNNNNNNNGG	5 cut(s)	464, 605, 932, 1310, 1338
BseMI	GCAATG	2 cut(s)	76, 165
BseMII	CTCAG	5 cut(s)	213, 858, 1182, 1299, 1421
BseNI	ACTGG	6 cut(s)	20, 143, 547, 553, 889, 1129
BseRI	GAGGAG	3 cut(s)	162, 174, 1240
BseSI	GKGCMC	3 cut(s)	586, 963, 1214
BseXI	GCAGC	2 cut(s)	295, 1074
BsgI	GTGCAG	1 cut(s)	1270
BshFI	GGCC	6 cut(s)	469, 481, 570, 584, 925, 1231
BsiHKAI	GWGCWC	5 cut(s)	9, 674, 963, 1110, 1320
BsiHKCI	CYCGRG	1 cut(s)	186
BsiSI	CCGG	4 cut(s)	58, 222, 461, 529
BsiWI	CGTACG	1 cut(s)	1215
BslFI	GGGAC	4 cut(s)	594, 696, 1069, 1366
BslI	CCNNNNNNNGG	5 cut(s)	464, 605, 932, 1310, 1338
BsmAI	GTCTC	4 cut(s)	985, 1034, 1072, 1355
BsmBI	CGTCTC	2 cut(s)	985, 1072
BsmFI	GGGAC	4 cut(s)	594, 696, 1069, 1366
BsmI	GAATGC	1 cut(s)	1348
BsnI	GGCC	6 cut(s)	469, 481, 570, 584, 925, 1231
Bso31I	GGTCTC	1 cut(s)	1034
BsoBI	CYCGRG	1 cut(s)	186
Bsp120I	GGGCCC	1 cut(s)	582
Bsp1286I	GDGCHC	7 cut(s)	9, 586, 674, 963, 1110, 1214, 1320
Bsp143I	GATC	6 cut(s)	33, 139, 617, 654, 804, 1327
Bsp1720I	GCTNAGC	1 cut(s)	785
BspACI	CCGC	4 cut(s)	379, 458, 997, 1053
BspANI	GGCC	6 cut(s)	469, 481, 570, 584, 925, 1231
BspCNI	CTCAG	5 cut(s)	212, 857, 1181, 1298, 1420
BspHI	TCATGA	1 cut(s)	666
BspLI	GGNNCC	7 cut(s)	553, 584, 609, 610, 806, 950, 1404
BspMAI	CTGCAG	1 cut(s)	136
BspPI	GGATC	6 cut(s)	41, 134, 625, 799, 812, 1322
BspTI	CTTAAG	1 cut(s)	694
BspTNI	GGTCTC	1 cut(s)	1034
BsrDI	GCAATG	2 cut(s)	76, 165
BsrI	ACTGG	6 cut(s)	20, 143, 547, 553, 889, 1129
BssECI	CCNNGG	4 cut(s)	220, 952, 969, 1260
BssMI	GATC	6 cut(s)	33, 139, 617, 654, 804, 1327
BssNAI	GTATAC	1 cut(s)	1434
BssT1I	CCWWGG	1 cut(s)	952
Bst1107I	GTATAC	1 cut(s)	1434
Bst2UI	CCWGG	2 cut(s)	423, 971
Bst4CI	ACNGT	7 cut(s)	251, 504, 710, 860, 994, 1028, 1261
Bst6I	CTCTTC	1 cut(s)	1085
BstAFI	CTTAAG	1 cut(s)	694
BstBAI	YACGTR	2 cut(s)	1013, 1215
BstC8I	GCNNGC	9 cut(s)	67, 471, 959, 976, 1118, 1153, 1249, 1267, 1391
BstDEI	CTNAG	6 cut(s)	199, 785, 844, 1168, 1285, 1407
BstDSI	CCRYGG	1 cut(s)	1260
BstF5I	GGATG	5 cut(s)	113, 537, 1074, 1089, 1330
BstKTI	GATC	6 cut(s)	36, 142, 620, 657, 807, 1330
BstMAI	GTCTC	4 cut(s)	985, 1034, 1072, 1355
BstMBI	GATC	6 cut(s)	33, 139, 617, 654, 804, 1327
BstMWI	GCNNNNNNNGC	4 cut(s)	466, 948, 1059, 1071
BstNI	CCWGG	2 cut(s)	423, 971
BstNSI	RCATGY	3 cut(s)	978, 1155, 1269
BstPAI	GACNNNNGTC	1 cut(s)	1026
BstSCI	CCNGG	3 cut(s)	220, 421, 969
BstSFI	CTRYAG	2 cut(s)	132, 1195
BstSLI	GKGCMC	3 cut(s)	586, 963, 1214
BstV1I	GCAGC	2 cut(s)	295, 1074
BstX2I	RGATCY	2 cut(s)	139, 804
BstXI	CCANNNNNNTGG	2 cut(s)	443, 477
BstYI	RGATCY	2 cut(s)	139, 804
BstZ17I	GTATAC	1 cut(s)	1434
Bsu36I	CCTNAGG	1 cut(s)	1407
BsuRI	GGCC	6 cut(s)	469, 481, 570, 584, 925, 1231
BtgI	CCRYGG	1 cut(s)	1260
BtrI	CACGTC	1 cut(s)	477
BtsCI	GGATG	5 cut(s)	113, 537, 1074, 1089, 1330
BtsI	GCAGTG	3 cut(s)	303, 623, 1258
BtsIMutI	CAGTG	7 cut(s)	13, 303, 509, 540, 623, 852, 1258
Cac8I	GCNNGC	9 cut(s)	67, 471, 959, 976, 1118, 1153, 1249, 1267, 1391
CciI	TCATGA	1 cut(s)	666
Cfr13I	GGNCC	7 cut(s)	410, 467, 582, 583, 608, 632, 923
CseI	GACGC	2 cut(s)	967, 1054
Csp6I	GTAC	5 cut(s)	546, 749, 1132, 1175, 1216
CspCI	CAANNNNNGTGG	4 cut(s)	54, 89, 560, 595
CviQI	GTAC	5 cut(s)	546, 749, 1132, 1175, 1216
DdeI	CTNAG	6 cut(s)	199, 785, 844, 1168, 1285, 1407
DpnI	GATC	6 cut(s)	35, 141, 619, 656, 806, 1329
DpnII	GATC	6 cut(s)	33, 139, 617, 654, 804, 1327
DriI	GACNNNNNGTC	1 cut(s)	1423
EaeI	YGGCCR	1 cut(s)	568
Eam1104I	CTCTTC	1 cut(s)	1085
Eam1105I	GACNNNNNGTC	1 cut(s)	1423
EarI	CTCTTC	1 cut(s)	1085
Ecl136II	GAGCTC	3 cut(s)	7, 672, 1318
Eco130I	CCWWGG	1 cut(s)	952
Eco147I	AGGCCT	1 cut(s)	1231
Eco24I	GRGCYC	4 cut(s)	9, 586, 674, 1320
Eco31I	GGTCTC	1 cut(s)	1034
Eco32I	GATATC	1 cut(s)	86
Eco47I	GGWCC	3 cut(s)	410, 608, 632
Eco53kI	GAGCTC	3 cut(s)	7, 672, 1318
Eco57I	CTGAAG	2 cut(s)	228, 354
Eco81I	CCTNAGG	1 cut(s)	1407
Eco88I	CYCGRG	1 cut(s)	186
EcoICRI	GAGCTC	3 cut(s)	7, 672, 1318
EcoO109I	RGGNCCY	2 cut(s)	583, 608
EcoRII	CCWGG	2 cut(s)	421, 969
EcoRV	GATATC	1 cut(s)	86
EcoT14I	CCWWGG	1 cut(s)	952
EcoT38I	GRGCYC	4 cut(s)	9, 586, 674, 1320
ErhI	CCWWGG	1 cut(s)	952
Esp3I	CGTCTC	2 cut(s)	985, 1072
FalI	AAGNNNNNCTT	2 cut(s)	1281, 1313
FaqI	GGGAC	4 cut(s)	594, 696, 1069, 1366
FauI	CCCGC	1 cut(s)	1046
FbaI	TGATCA	1 cut(s)	654
FblI	GTMKAC	2 cut(s)	1125, 1433
Fnu4HI	GCNGC	3 cut(s)	309, 458, 1063
FokI	GGATG	5 cut(s)	100, 544, 1061, 1076, 1337
FriOI	GRGCYC	4 cut(s)	9, 586, 674, 1320
Fsp4HI	GCNGC	3 cut(s)	309, 458, 1063
FspBI	CTAG	3 cut(s)	428, 1121, 1178
GluI	GCNGC	3 cut(s)	309, 458, 1063
HaeIII	GGCC	6 cut(s)	469, 481, 570, 584, 925, 1231
HapII	CCGG	4 cut(s)	58, 222, 461, 529
HgaI	GACGC	2 cut(s)	967, 1054
HindIII	AAGCTT	1 cut(s)	782
HinfI	GANTC	6 cut(s)	24, 43, 699, 754, 1016, 1035
HpaII	CCGG	4 cut(s)	58, 222, 461, 529
HphI	GGTGA	5 cut(s)	83, 382, 697, 768, 872
Hpy166II	GTNNAC	3 cut(s)	961, 1126, 1434
Hpy188I	TCNGA	9 cut(s)	162, 202, 247, 373, 659, 759, 1021, 1034, 1288
Hpy188III	TCNNGA	7 cut(s)	188, 667, 703, 874, 1039, 1084, 1093
Hpy8I	GTNNAC	3 cut(s)	961, 1126, 1434
Hpy99I	CGWCG	1 cut(s)	478
HpyAV	CCTTC	6 cut(s)	64, 71, 162, 378, 554, 1304
HpyCH4III	ACNGT	7 cut(s)	251, 504, 710, 860, 994, 1028, 1261
HpyCH4IV	ACGT	4 cut(s)	261, 476, 1012, 1214
HpyF10VI	GCNNNNNNNGC	4 cut(s)	466, 948, 1059, 1071
HpyF3I	CTNAG	6 cut(s)	199, 785, 844, 1168, 1285, 1407
HpySE526I	ACGT	4 cut(s)	261, 476, 1012, 1214
KflI	GGGWCCC	1 cut(s)	608
Ksp22I	TGATCA	1 cut(s)	654
Kzo9I	GATC	6 cut(s)	33, 139, 617, 654, 804, 1327
LmnI	GCTCC	5 cut(s)	4, 154, 175, 370, 948
Lsp1109I	GCAGC	2 cut(s)	295, 1074
LweI	GCATC	3 cut(s)	444, 879, 1083
MaeI	CTAG	3 cut(s)	428, 1121, 1178
MaeII	ACGT	4 cut(s)	261, 476, 1012, 1214
MaeIII	GTNAC	6 cut(s)	563, 638, 913, 929, 1180, 1253
MalI	GATC	6 cut(s)	35, 141, 619, 656, 806, 1329
MboI	GATC	6 cut(s)	33, 139, 617, 654, 804, 1327
MboII	GAAGA	5 cut(s)	93, 149, 234, 765, 1072
MflI	RGATCY	2 cut(s)	139, 804
MhlI	GDGCHC	7 cut(s)	9, 586, 674, 963, 1110, 1214, 1320
MluCI	AATT	5 cut(s)	238, 399, 676, 716, 728
MlyI	GAGTC	2 cut(s)	18, 37
MmeI	TCCRAC	4 cut(s)	262, 1008, 1044, 1319
MseI	TTAA	3 cut(s)	345, 525, 695
MslI	CAYNNNNRTG	2 cut(s)	1094, 1150
MspCI	CTTAAG	1 cut(s)	694
MspI	CCGG	4 cut(s)	58, 222, 461, 529
MspR9I	CCNGG	3 cut(s)	222, 423, 971
Mva1269I	GAATGC	1 cut(s)	1348
MvaI	CCWGG	2 cut(s)	423, 971
MwoI	GCNNNNNNNGC	4 cut(s)	466, 948, 1059, 1071
NciI	CCSGG	1 cut(s)	222
NdeII	GATC	6 cut(s)	33, 139, 617, 654, 804, 1327
NlaIV	GGNNCC	7 cut(s)	553, 584, 609, 610, 806, 950, 1404
NmuCI	GTSAC	5 cut(s)	638, 913, 929, 1180, 1253
NspI	RCATGY	3 cut(s)	978, 1155, 1269
PaeI	GCATGC	3 cut(s)	978, 1155, 1269
PaeR7I	CTCGAG	1 cut(s)	186
PagI	TCATGA	1 cut(s)	666
PceI	AGGCCT	1 cut(s)	1231
PctI	GAATGC	1 cut(s)	1348
PfeI	GAWTC	4 cut(s)	699, 754, 1016, 1035
Pfl23II	CGTACG	1 cut(s)	1215
PkrI	GCNGC	3 cut(s)	310, 459, 1064
PleI	GAGTC	2 cut(s)	18, 37
PpsI	GAGTC	2 cut(s)	18, 37
Ppu21I	YACGTR	2 cut(s)	1013, 1215
PpuMI	RGGWCCY	1 cut(s)	608
PshAI	GACNNNNGTC	1 cut(s)	1026
Psp124BI	GAGCTC	3 cut(s)	9, 674, 1320
Psp5II	RGGWCCY	1 cut(s)	608
Psp6I	CCWGG	2 cut(s)	421, 969
PspGI	CCWGG	2 cut(s)	421, 969
PspLI	CGTACG	1 cut(s)	1215
PspN4I	GGNNCC	7 cut(s)	553, 584, 609, 610, 806, 950, 1404
PspOMI	GGGCCC	1 cut(s)	582
PspPI	GGNCC	7 cut(s)	410, 467, 582, 583, 608, 632, 923
PspPPI	RGGWCCY	1 cut(s)	608
PstI	CTGCAG	1 cut(s)	136
PsuI	RGATCY	2 cut(s)	139, 804
RsaI	GTAC	5 cut(s)	547, 750, 1133, 1176, 1217
RsaNI	GTAC	5 cut(s)	546, 749, 1132, 1175, 1216
RseI	CAYNNNNRTG	2 cut(s)	1094, 1150
SacI	GAGCTC	3 cut(s)	9, 674, 1320
SaqAI	TTAA	3 cut(s)	345, 525, 695
SatI	GCNGC	3 cut(s)	309, 458, 1063
Sau3AI	GATC	6 cut(s)	33, 139, 617, 654, 804, 1327
Sau96I	GGNCC	7 cut(s)	410, 467, 582, 583, 608, 632, 923
ScaI	AGTACT	3 cut(s)	750, 1133, 1176
SchI	GAGTC	2 cut(s)	18, 37
ScrFI	CCNGG	3 cut(s)	222, 423, 971
SduI	GDGCHC	7 cut(s)	9, 586, 674, 963, 1110, 1214, 1320
SfaNI	GCATC	3 cut(s)	444, 879, 1083
SfcI	CTRYAG	2 cut(s)	132, 1195
Sfr274I	CTCGAG	1 cut(s)	186
SinI	GGWCC	3 cut(s)	410, 608, 632
SlaI	CTCGAG	1 cut(s)	186
SmiMI	CAYNNNNRTG	2 cut(s)	1094, 1150
SmlI	CTYRAG	2 cut(s)	186, 694
SmoI	CTYRAG	2 cut(s)	186, 694
SpeI	ACTAGT	2 cut(s)	427, 1177
SphI	GCATGC	3 cut(s)	978, 1155, 1269
Sse9I	AATT	5 cut(s)	238, 399, 676, 716, 728
SseBI	AGGCCT	1 cut(s)	1231
SsiI	CCGC	4 cut(s)	379, 458, 997, 1053
SspI	AATATT	1 cut(s)	1276
SspMI	CTAG	3 cut(s)	428, 1121, 1178
SstI	GAGCTC	3 cut(s)	9, 674, 1320
StuI	AGGCCT	1 cut(s)	1231
StyD4I	CCNGG	3 cut(s)	220, 421, 969
StyI	CCWWGG	1 cut(s)	952
TaaI	ACNGT	7 cut(s)	251, 504, 710, 860, 994, 1028, 1261
TaiI	ACGT	4 cut(s)	264, 479, 1015, 1217
TaqI	TCGA	4 cut(s)	187, 732, 875, 1083
TasI	AATT	5 cut(s)	238, 399, 676, 716, 728
TatI	WGTACW	3 cut(s)	748, 1131, 1174
TauI	GCSGC	1 cut(s)	460
TfiI	GAWTC	4 cut(s)	699, 754, 1016, 1035
Tru1I	TTAA	3 cut(s)	345, 525, 695
Tru9I	TTAA	3 cut(s)	345, 525, 695
TscAI	CASTG	7 cut(s)	20, 310, 509, 547, 630, 852, 1258
TseFI	GTSAC	5 cut(s)	638, 913, 929, 1180, 1253
TseI	GCWGC	2 cut(s)	308, 1062
Tsp45I	GTSAC	5 cut(s)	638, 913, 929, 1180, 1253
TspDTI	ATGAA	2 cut(s)	655, 740
TspGWI	ACGGA	1 cut(s)	994
TspRI	CASTG	7 cut(s)	20, 310, 509, 547, 630, 852, 1258
Vha464I	CTTAAG	1 cut(s)	694
VneI	GTGCAC	1 cut(s)	959
VpaK11BI	GGWCC	3 cut(s)	410, 608, 632
XapI	RAATTY	1 cut(s)	728
XceI	RCATGY	3 cut(s)	978, 1155, 1269
XhoI	CTCGAG	1 cut(s)	186
XmiI	GTMKAC	2 cut(s)	1125, 1433
XspI	CTAG	3 cut(s)	428, 1121, 1178
ZrmI	AGTACT	3 cut(s)	750, 1133, 1176

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.