MD13G1146600.v1.1

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008992
Physical Location & Seq
Reverse (-)
11426282 .. 11430248
3967 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1146600.v1.1.491

Sequence Viewer

Length: 1767 bp
ATGGAGCTCGTTCACTGGTTTAGAGTCTTATGGATCACAATGACTCTATTAACCTTCCAGTATGCAAGCAATGTGGAAGGAAGATATCATTACCACAAGAGAAACAAAAGCGCATCCCCTCCTCCTACCCCTGCAGAAGATCGGGGTTCTCCATCACCCCCAAGCATTGCTCCTCCTACCCTTGTAGAAAAACCAGTTGCTCCTACCCCTGCAGAAGATCGGGGTTCTCCATCACCCCCAAGCATTGCTCCTCCTACCCCTGCAGAAGAACCATTTGCTCCTCCTATCCCTGCAGAAGAGCCACCAGCTGCTCCTTCAACCCCGAGCATTGCTCCTCCTACCCCTGTAGAAGAACCAGTTGCTCCTCCTACCCCTGCAGAAAAGCCAGCTGCTCCTTCAACCCCGAGCATTGCTCCTGCTACCCCTGTAGAAGAACCAGTTGCTCCTCCTACCCCTGCAGAAAAGCCAGTTGCTCCTTCAACCCCGAGCATTACTCCTCCTACCCCTCCTCCACATGTTCCCTCAGACCCTTACCCAAATGACCCTGGAAACAACTCTAGTAATTCTTCAGATTGTGTGTTTAATGTCATGGATTATGGGGCAGTTGGAGATGGCTCTGCTGATGACACTGCTGCCTTTAGACAGGCTTGGAAGGAAGCCTGTGCTGTTGAATCAGGTGTTGTTTTGGCTCCTTCTGATTACTGCTTCAAAATCACCTCAACAATTTTTTCAGGTCCATGCCAGCCAGGACTAGTATTCCAAGTAGATGGGGTTTTGATGCCGCCGGACGGGCCAGAGACGTGGCCTACAACAGACAGCCTAAACCAGTGGCTTGTGTTTTACAGACTCGACCAAATGACTTTCACTGGTACTGGAACCATCGAAGGCAACGGCCAAAAGTGGTGGGAGCTCCCTTGCAAACCTCACAGGGGTCCCAAAGGATCAACACTGCCAGGACCATGTGACAGTCCTGCGATGATCAGATTTTTCATGAGCTCTAATTTGGTGGTGAGAGGCTTAAGAATCCAAAACAGTCCAAAGTTCCACATGAAATTCGATGGCTGTGAAGGAGTACTGATTGAGAAAATATCCATTTCTTCACCAAAGCTTAGCCCCAACACAGATGGGATCCACATAGAGAACACAAAATCTGTTGCAATATACGACTCAATGATTAGCAACGGTGATGACTGCATTTCAATAGGAACTGGGTGTGCAAATGTTGATATAATGGGGGTCACTTGCGGGCCAAGTCACGGGATTAGCATTGGGAGCCTTGGCGTGCACAACTCTCGGGCATGCGTCTCCAACATCACAGTCCGCAACGCAGTCATACGTGAATCAGACAATGGCGCCCGAATCAAGACATGGCAAGGCGGGACGGGCTGCGTATCTGGCATCCTCTTCGAGAACATCCAGATGGAGAATGTGCTCAACTGCCTGCTAGTAGACCAGTACTACTGCCTATCAAAGGGATGCCTAAACCAAACCTCAGCAGTACTAGTCACAGATTTAACATACAGAAACATAAAGGGGACGTACGATGTGAGGACGCCTCCCATTCACTTTGCATGCAGTGACACCGTAGCATGCAGAAATATTACCCTCTCAGAAGTTGAGCTTTACCCACACGAAGGAGAACTCATGGATGATCCCTTTTGTTGGAATGCTTATGGAATCCAAGAGACTGCTACTATTCCCCCTATTGATTGCTTGCAAGAGGGGGAACCTCAGGTTGCGGCTGAGGTGTCTGAGTATACTTGCTAG

Protein Analysis

589

Amino Acids

62.93

Weight (kDa)

4.71

Isoelectric Point (pI)

57.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 231 - 541 1.4e-80 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1352
AccI GTMKAC 2 cut(s) 1449, 1757
AciI CCGC 5 cut(s) 782, 1245, 1321, 1377, 1739
AclWI GGATC 5 cut(s) 41, 949, 1123, 1136, 1646
AcoI YGGCCR 1 cut(s) 892
AcsI RAATTY 1 cut(s) 1052
AcuI CTGAAG 1 cut(s) 552
AcyI GRCGYC 2 cut(s) 1353, 1553
AfaI GTAC 5 cut(s) 871, 1074, 1457, 1500, 1541
AfiI CCNNNNNNNGG 6 cut(s) 788, 929, 1256, 1471, 1634, 1662
AflII CTTAAG 1 cut(s) 1018
AflIII ACRYGT 1 cut(s) 514
AgsI TTSAA 6 cut(s) 318, 399, 480, 671, 709, 1200
AhlI ACTAGT 2 cut(s) 751, 1501
AjiI CACGTC 1 cut(s) 801
AjnI CCWGG 3 cut(s) 544, 745, 952
AluBI AGCT 7 cut(s) 7, 308, 389, 910, 996, 1108, 1621
AluI AGCT 7 cut(s) 7, 308, 389, 910, 996, 1108, 1621
Alw21I GWGCWC 5 cut(s) 9, 912, 998, 1287, 1434
Alw26I GTCTC 3 cut(s) 791, 1309, 1679
Alw44I GTGCAC 1 cut(s) 1283
AlwI GGATC 5 cut(s) 41, 949, 1123, 1136, 1646
Ama87I CYCGRG 4 cut(s) 322, 403, 484, 1293
AoxI GGCC 4 cut(s) 791, 803, 892, 1247
ApaLI GTGCAC 1 cut(s) 1283
ApeKI GCWGC 4 cut(s) 308, 389, 632, 1386
ApoI RAATTY 1 cut(s) 1052
AspLEI GCGC 2 cut(s) 113, 1355
AspS9I GGNCC 5 cut(s) 734, 791, 932, 956, 1247
AsuHPI GGTGA 6 cut(s) 147, 225, 706, 1021, 1092, 1196
AvaI CYCGRG 4 cut(s) 322, 403, 484, 1293
AvaII GGWCC 3 cut(s) 734, 932, 956
AxyI CCTNAGG 1 cut(s) 1731
BaeGI GKGCMC 1 cut(s) 1287
BamHI GGATCC 1 cut(s) 1128
BanI GGYRCC 1 cut(s) 1352
BanII GRGCYC 3 cut(s) 9, 912, 998
Bbv12I GWGCWC 5 cut(s) 9, 912, 998, 1287, 1434
BbvCI CCTCAGC 2 cut(s) 1492, 1743
BbvI GCAGC 4 cut(s) 295, 376, 619, 1373
BccI CCATC 8 cut(s) 160, 238, 605, 761, 887, 1052, 1118, 1414
BceAI ACGGC 1 cut(s) 907
BcgI CGANNNNNNTGC 4 cut(s) 1274, 1308, 1387, 1421
BciT130I CCWGG 3 cut(s) 546, 747, 954
BclI TGATCA 1 cut(s) 978
BcoDI GTCTC 3 cut(s) 791, 1309, 1679
BcuI ACTAGT 2 cut(s) 751, 1501
BfaI CTAG 5 cut(s) 558, 752, 1445, 1502, 1765
BfmI CTRYAG 8 cut(s) 132, 210, 261, 291, 345, 375, 426, 456
BfoI RGCGCY 1 cut(s) 1356
BfrI CTTAAG 1 cut(s) 1018
BglI GCCNNNNNGGC 1 cut(s) 790
BisI GCNGC 6 cut(s) 309, 390, 633, 782, 1387, 1740
BlpI GCTNAGC 1 cut(s) 1109
BlsI GCNGC 6 cut(s) 310, 391, 634, 783, 1388, 1741
BmcAI AGTACT 3 cut(s) 1074, 1457, 1500
Bme1390I CCNGG 3 cut(s) 546, 747, 954
Bme18I GGWCC 3 cut(s) 734, 932, 956
BmeT110I CYCGRG 4 cut(s) 322, 403, 484, 1293
BmgBI CACGTC 1 cut(s) 801
BmgT120I GGNCC 5 cut(s) 734, 791, 932, 956, 1247
BmiI GGNNCC 8 cut(s) 690, 877, 933, 934, 1130, 1274, 1354, 1728
BmrFI CCNGG 3 cut(s) 546, 747, 954
BmrI ACTGGG 1 cut(s) 1218
BmsI GCATC 4 cut(s) 122, 768, 1407, 1466
BmuI ACTGGG 1 cut(s) 1218
BplI GAGNNNNNCTC 8 cut(s) 316, 348, 397, 429, 478, 510, 1540, 1572
Bpu10I CCTNAGC 2 cut(s) 1492, 1743
Bpu1102I GCTNAGC 1 cut(s) 1109
BsaAI YACGTR 1 cut(s) 1337
BsaHI GRCGYC 2 cut(s) 1353, 1553
BsaJI CCNNGG 2 cut(s) 544, 1276
BsaXI ACNNNNNCTCC 2 cut(s) 493, 523
Bsc4I CCNNNNNNNGG 6 cut(s) 788, 929, 1256, 1471, 1634, 1662
Bse21I CCTNAGG 1 cut(s) 1731
Bse3DI GCAATG 5 cut(s) 76, 165, 243, 327, 408
BseBI CCWGG 3 cut(s) 546, 747, 954
BseDI CCNNGG 2 cut(s) 544, 1276
BseGI GGATG 5 cut(s) 113, 1398, 1413, 1481, 1654
BseLI CCNNNNNNNGG 6 cut(s) 788, 929, 1256, 1471, 1634, 1662
BseMI GCAATG 5 cut(s) 76, 165, 243, 327, 408
BseMII CTCAG 6 cut(s) 537, 1506, 1623, 1734, 1743, 1745
BseRI GAGGAG 9 cut(s) 111, 162, 240, 270, 324, 354, 435, 486, 498
BseSI GKGCMC 1 cut(s) 1287
BseXI GCAGC 4 cut(s) 295, 376, 619, 1373
BshFI GGCC 4 cut(s) 793, 805, 894, 1249
BshNI GGYRCC 1 cut(s) 1352
BsiHKAI GWGCWC 5 cut(s) 9, 912, 998, 1287, 1434
BsiHKCI CYCGRG 4 cut(s) 322, 403, 484, 1293
BsiSI CCGG 1 cut(s) 785
BsiWI CGTACG 1 cut(s) 1539
BslFI GGGAC 3 cut(s) 918, 1393, 1549
BslI CCNNNNNNNGG 6 cut(s) 788, 929, 1256, 1471, 1634, 1662
BsmAI GTCTC 3 cut(s) 791, 1309, 1679
BsmBI CGTCTC 2 cut(s) 791, 1309
BsmFI GGGAC 3 cut(s) 918, 1393, 1549
BsmI GAATGC 1 cut(s) 1672
BsnI GGCC 4 cut(s) 793, 805, 894, 1249
BsoBI CYCGRG 4 cut(s) 322, 403, 484, 1293
Bsp1286I GDGCHC 5 cut(s) 9, 912, 998, 1287, 1434
Bsp143I GATC 7 cut(s) 33, 139, 217, 941, 978, 1128, 1651
Bsp1720I GCTNAGC 1 cut(s) 1109
BspACI CCGC 5 cut(s) 782, 1245, 1321, 1377, 1739
BspANI GGCC 4 cut(s) 793, 805, 894, 1249
BspCNI CTCAG 6 cut(s) 536, 1505, 1622, 1735, 1744, 1744
BspHI TCATGA 1 cut(s) 990
BspLI GGNNCC 8 cut(s) 690, 877, 933, 934, 1130, 1274, 1354, 1728
BspMAI CTGCAG 6 cut(s) 136, 214, 265, 295, 379, 460
BspPI GGATC 5 cut(s) 41, 949, 1123, 1136, 1646
BspQI GCTCTTC 1 cut(s) 291
BspT107I GGYRCC 1 cut(s) 1352
BspTI CTTAAG 1 cut(s) 1018
BsrDI GCAATG 5 cut(s) 76, 165, 243, 327, 408
BssECI CCNNGG 2 cut(s) 544, 1276
BssMI GATC 7 cut(s) 33, 139, 217, 941, 978, 1128, 1651
BssNAI GTATAC 1 cut(s) 1758
BssNI GRCGYC 2 cut(s) 1353, 1553
BssT1I CCWWGG 1 cut(s) 1276
Bst1107I GTATAC 1 cut(s) 1758
Bst2UI CCWGG 3 cut(s) 546, 747, 954
Bst4CI ACNGT 5 cut(s) 968, 1034, 1184, 1318, 1585
Bst6I CTCTTC 2 cut(s) 291, 1409
BstACI GRCGYC 2 cut(s) 1353, 1553
BstAFI CTTAAG 1 cut(s) 1018
BstBAI YACGTR 1 cut(s) 1337
BstDEI CTNAG 7 cut(s) 523, 1109, 1492, 1609, 1731, 1743, 1752
BstENI CCTNNNNNAGG 1 cut(s) 1469
BstF5I GGATG 5 cut(s) 113, 1398, 1413, 1481, 1654
BstH2I RGCGCY 1 cut(s) 1356
BstHHI GCGC 2 cut(s) 113, 1355
BstKTI GATC 7 cut(s) 36, 142, 220, 944, 981, 1131, 1654
BstMAI GTCTC 3 cut(s) 791, 1309, 1679
BstMBI GATC 7 cut(s) 33, 139, 217, 941, 978, 1128, 1651
BstMWI GCNNNNNNNGC 4 cut(s) 790, 1272, 1383, 1395
BstNI CCWGG 3 cut(s) 546, 747, 954
BstNSI RCATGY 4 cut(s) 518, 1302, 1575, 1593
BstSCI CCNGG 3 cut(s) 544, 745, 952
BstSFI CTRYAG 8 cut(s) 132, 210, 261, 291, 345, 375, 426, 456
BstSLI GKGCMC 1 cut(s) 1287
BstV1I GCAGC 4 cut(s) 295, 376, 619, 1373
BstX2I RGATCY 1 cut(s) 1128
BstXI CCANNNNNNTGG 2 cut(s) 767, 801
BstYI RGATCY 1 cut(s) 1128
BstZ17I GTATAC 1 cut(s) 1758
Bsu36I CCTNAGG 1 cut(s) 1731
BsuRI GGCC 4 cut(s) 793, 805, 894, 1249
BtgZI GCGATG 1 cut(s) 989
BtrI CACGTC 1 cut(s) 801
BtsCI GGATG 5 cut(s) 113, 1398, 1413, 1481, 1654
BtsI GCAGTG 3 cut(s) 627, 947, 1582
BtsIMutI CAGTG 6 cut(s) 13, 627, 833, 864, 947, 1582
CciI TCATGA 1 cut(s) 990
CfoI GCGC 2 cut(s) 113, 1355
Cfr13I GGNCC 5 cut(s) 734, 791, 932, 956, 1247
CseI GACGC 2 cut(s) 1291, 1561
Csp6I GTAC 5 cut(s) 870, 1073, 1456, 1499, 1540
CspCI CAANNNNNGTGG 4 cut(s) 54, 89, 884, 919
CviQI GTAC 5 cut(s) 870, 1073, 1456, 1499, 1540
DdeI CTNAG 7 cut(s) 523, 1109, 1492, 1609, 1731, 1743, 1752
DinI GGCGCC 1 cut(s) 1354
DpnI GATC 7 cut(s) 35, 141, 219, 943, 980, 1130, 1653
DpnII GATC 7 cut(s) 33, 139, 217, 941, 978, 1128, 1651
EaeI YGGCCR 1 cut(s) 892
Eam1104I CTCTTC 2 cut(s) 291, 1409
EarI CTCTTC 2 cut(s) 291, 1409
Ecl136II GAGCTC 3 cut(s) 7, 910, 996
Eco130I CCWWGG 1 cut(s) 1276
Eco24I GRGCYC 3 cut(s) 9, 912, 998
Eco32I GATATC 1 cut(s) 86
Eco47I GGWCC 3 cut(s) 734, 932, 956
Eco53kI GAGCTC 3 cut(s) 7, 910, 996
Eco57I CTGAAG 1 cut(s) 552
Eco81I CCTNAGG 1 cut(s) 1731
Eco88I CYCGRG 4 cut(s) 322, 403, 484, 1293
EcoICRI GAGCTC 3 cut(s) 7, 910, 996
EcoNI CCTNNNNNAGG 1 cut(s) 1469
EcoO109I RGGNCCY 1 cut(s) 932
EcoRII CCWGG 3 cut(s) 544, 745, 952
EcoRV GATATC 1 cut(s) 86
EcoT14I CCWWGG 1 cut(s) 1276
EcoT38I GRGCYC 3 cut(s) 9, 912, 998
EgeI GGCGCC 1 cut(s) 1354
EheI GGCGCC 1 cut(s) 1354
ErhI CCWWGG 1 cut(s) 1276
Esp3I CGTCTC 2 cut(s) 791, 1309
FalI AAGNNNNNCTT 2 cut(s) 1605, 1637
FaqI GGGAC 3 cut(s) 918, 1393, 1549
FauI CCCGC 2 cut(s) 1238, 1370
FbaI TGATCA 1 cut(s) 978
FblI GTMKAC 2 cut(s) 1449, 1757
Fnu4HI GCNGC 6 cut(s) 309, 390, 633, 782, 1387, 1740
FokI GGATG 5 cut(s) 100, 1385, 1400, 1488, 1661
FriOI GRGCYC 3 cut(s) 9, 912, 998
Fsp4HI GCNGC 6 cut(s) 309, 390, 633, 782, 1387, 1740
FspBI CTAG 5 cut(s) 558, 752, 1445, 1502, 1765
GlaI GCGC 2 cut(s) 112, 1354
GluI GCNGC 6 cut(s) 309, 390, 633, 782, 1387, 1740
HaeII RGCGCY 1 cut(s) 1356
HaeIII GGCC 4 cut(s) 793, 805, 894, 1249
HapII CCGG 1 cut(s) 785
HgaI GACGC 2 cut(s) 1291, 1561
HhaI GCGC 2 cut(s) 113, 1355
Hin1I GRCGYC 2 cut(s) 1353, 1553
Hin6I GCGC 2 cut(s) 111, 1353
HinP1I GCGC 2 cut(s) 111, 1353
HindIII AAGCTT 1 cut(s) 1106
HinfI GANTC 9 cut(s) 24, 43, 671, 846, 1023, 1166, 1340, 1359, 1677
HpaII CCGG 1 cut(s) 785
HphI GGTGA 6 cut(s) 147, 225, 706, 1021, 1092, 1196
Hpy166II GTNNAC 4 cut(s) 13, 1285, 1450, 1758
Hpy188I TCNGA 7 cut(s) 526, 571, 697, 983, 1345, 1612, 1753
Hpy188III TCNNGA 4 cut(s) 991, 1363, 1408, 1417
Hpy8I GTNNAC 4 cut(s) 13, 1285, 1450, 1758
HpyCH4III ACNGT 5 cut(s) 968, 1034, 1184, 1318, 1585
HpyCH4IV ACGT 3 cut(s) 800, 1336, 1538
HpyF10VI GCNNNNNNNGC 4 cut(s) 790, 1272, 1383, 1395
HpyF3I CTNAG 7 cut(s) 523, 1109, 1492, 1609, 1731, 1743, 1752
HpySE526I ACGT 3 cut(s) 800, 1336, 1538
Hsp92I GRCGYC 2 cut(s) 1353, 1553
HspAI GCGC 2 cut(s) 111, 1353
KasI GGCGCC 1 cut(s) 1352
KflI GGGWCCC 1 cut(s) 932
Ksp22I TGATCA 1 cut(s) 978
Kzo9I GATC 7 cut(s) 33, 139, 217, 941, 978, 1128, 1651
LguI GCTCTTC 1 cut(s) 291
Lsp1109I GCAGC 4 cut(s) 295, 376, 619, 1373
LweI GCATC 4 cut(s) 122, 768, 1407, 1466
MaeI CTAG 5 cut(s) 558, 752, 1445, 1502, 1765
MaeII ACGT 3 cut(s) 800, 1336, 1538
MaeIII GTNAC 5 cut(s) 962, 1237, 1253, 1504, 1577
MalI GATC 7 cut(s) 35, 141, 219, 943, 980, 1130, 1653
MboI GATC 7 cut(s) 33, 139, 217, 941, 978, 1128, 1651
MflI RGATCY 1 cut(s) 1128
MhlI GDGCHC 5 cut(s) 9, 912, 998, 1287, 1434
MluCI AATT 4 cut(s) 562, 723, 1000, 1052
Mly113I GGCGCC 1 cut(s) 1353
MlyI GAGTC 4 cut(s) 33, 37, 840, 1160
MmeI TCCRAC 3 cut(s) 586, 1332, 1643
MseI TTAA 4 cut(s) 50, 582, 1019, 1514
MslI CAYNNNNRTG 1 cut(s) 1418
MspA1I CMGCKG 2 cut(s) 308, 389
MspCI CTTAAG 1 cut(s) 1018
MspI CCGG 1 cut(s) 785
MspR9I CCNGG 3 cut(s) 546, 747, 954
Mva1269I GAATGC 1 cut(s) 1672
MvaI CCWGG 3 cut(s) 546, 747, 954
MwoI GCNNNNNNNGC 4 cut(s) 790, 1272, 1383, 1395
NarI GGCGCC 1 cut(s) 1353
NdeII GATC 7 cut(s) 33, 139, 217, 941, 978, 1128, 1651
NlaIV GGNNCC 8 cut(s) 690, 877, 933, 934, 1130, 1274, 1354, 1728
NmuCI GTSAC 5 cut(s) 962, 1237, 1253, 1504, 1577
NspI RCATGY 4 cut(s) 518, 1302, 1575, 1593
PaeI GCATGC 3 cut(s) 1302, 1575, 1593
PagI TCATGA 1 cut(s) 990
PciI ACATGT 1 cut(s) 514
PciSI GCTCTTC 1 cut(s) 291
PctI GAATGC 1 cut(s) 1672
PfeI GAWTC 5 cut(s) 671, 1023, 1340, 1359, 1677
Pfl23II CGTACG 1 cut(s) 1539
PkrI GCNGC 6 cut(s) 310, 391, 634, 783, 1388, 1741
PleI GAGTC 4 cut(s) 32, 37, 840, 1160
PluTI GGCGCC 1 cut(s) 1356
PpsI GAGTC 4 cut(s) 32, 37, 840, 1160
Ppu21I YACGTR 1 cut(s) 1337
PpuMI RGGWCCY 1 cut(s) 932
PscI ACATGT 1 cut(s) 514
Psp124BI GAGCTC 3 cut(s) 9, 912, 998
Psp5II RGGWCCY 1 cut(s) 932
Psp6I CCWGG 3 cut(s) 544, 745, 952
PspGI CCWGG 3 cut(s) 544, 745, 952
PspLI CGTACG 1 cut(s) 1539
PspN4I GGNNCC 8 cut(s) 690, 877, 933, 934, 1130, 1274, 1354, 1728
PspPI GGNCC 5 cut(s) 734, 791, 932, 956, 1247
PspPPI RGGWCCY 1 cut(s) 932
PstI CTGCAG 6 cut(s) 136, 214, 265, 295, 379, 460
PsuI RGATCY 1 cut(s) 1128
PvuII CAGCTG 2 cut(s) 308, 389
RsaI GTAC 5 cut(s) 871, 1074, 1457, 1500, 1541
RsaNI GTAC 5 cut(s) 870, 1073, 1456, 1499, 1540
RseI CAYNNNNRTG 1 cut(s) 1418
SacI GAGCTC 3 cut(s) 9, 912, 998
SapI GCTCTTC 1 cut(s) 291
SaqAI TTAA 4 cut(s) 50, 582, 1019, 1514
SatI GCNGC 6 cut(s) 309, 390, 633, 782, 1387, 1740
Sau3AI GATC 7 cut(s) 33, 139, 217, 941, 978, 1128, 1651
Sau96I GGNCC 5 cut(s) 734, 791, 932, 956, 1247
ScaI AGTACT 3 cut(s) 1074, 1457, 1500
SchI GAGTC 4 cut(s) 33, 37, 840, 1160
ScrFI CCNGG 3 cut(s) 546, 747, 954
SduI GDGCHC 5 cut(s) 9, 912, 998, 1287, 1434
SfaNI GCATC 4 cut(s) 122, 768, 1407, 1466
SfcI CTRYAG 8 cut(s) 132, 210, 261, 291, 345, 375, 426, 456
SfoI GGCGCC 1 cut(s) 1354
SinI GGWCC 3 cut(s) 734, 932, 956
SmiMI CAYNNNNRTG 1 cut(s) 1418
SmlI CTYRAG 1 cut(s) 1018
SmoI CTYRAG 1 cut(s) 1018
SpeI ACTAGT 2 cut(s) 751, 1501
SphI GCATGC 3 cut(s) 1302, 1575, 1593
Sse9I AATT 4 cut(s) 562, 723, 1000, 1052
SsiI CCGC 5 cut(s) 782, 1245, 1321, 1377, 1739
SspDI GGCGCC 1 cut(s) 1352
SspI AATATT 1 cut(s) 1600
SspMI CTAG 5 cut(s) 558, 752, 1445, 1502, 1765
SstI GAGCTC 3 cut(s) 9, 912, 998
StyD4I CCNGG 3 cut(s) 544, 745, 952
StyI CCWWGG 1 cut(s) 1276
TaaI ACNGT 5 cut(s) 968, 1034, 1184, 1318, 1585
TaiI ACGT 3 cut(s) 803, 1339, 1541
TaqI TCGA 4 cut(s) 849, 882, 1056, 1407
TasI AATT 4 cut(s) 562, 723, 1000, 1052
TatI WGTACW 3 cut(s) 1072, 1455, 1498
TauI GCSGC 2 cut(s) 784, 1742
TfiI GAWTC 5 cut(s) 671, 1023, 1340, 1359, 1677
Tru1I TTAA 4 cut(s) 50, 582, 1019, 1514
Tru9I TTAA 4 cut(s) 50, 582, 1019, 1514
TscAI CASTG 6 cut(s) 20, 634, 833, 871, 954, 1582
TseFI GTSAC 5 cut(s) 962, 1237, 1253, 1504, 1577
TseI GCWGC 4 cut(s) 308, 389, 632, 1386
Tsp45I GTSAC 5 cut(s) 962, 1237, 1253, 1504, 1577
TspDTI ATGAA 2 cut(s) 979, 1064
TspRI CASTG 6 cut(s) 20, 634, 833, 871, 954, 1582
Vha464I CTTAAG 1 cut(s) 1018
VneI GTGCAC 1 cut(s) 1283
VpaK11BI GGWCC 3 cut(s) 734, 932, 956
XagI CCTNNNNNAGG 1 cut(s) 1469
XapI RAATTY 1 cut(s) 1052
XceI RCATGY 4 cut(s) 518, 1302, 1575, 1593
XmiI GTMKAC 2 cut(s) 1449, 1757
XspI CTAG 5 cut(s) 558, 752, 1445, 1502, 1765
ZrmI AGTACT 3 cut(s) 1074, 1457, 1500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.