FvH4_4g23820

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015603
Physical Location & Seq
Reverse (-)
26186161 .. 26189152
2992 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23820.t1

Sequence Viewer

Length: 1569 bp
ATGAGACTCTTCATAATAGCCAACTTCTCCATCCTACTCTTTTGGTTTGGAGCTTCCACTCTCACTCTCACTCTCACTCTCCTTTCATTTTTCTTCTATGTCTTCCTCTTCGTAACAAGTTACTACCTCGTTCCCGGAGGCTCTGCATGGTCCGGTTACGGCCATGCCATGAAACTTCGAGGACCGGTAGGGTGGCCCGTATTGGGTTCCATACCCGAGCAAATGAGCTCTCTTGCTCATCGTAGACTAGCCAGCATGGCAGCTTCGTTGGATGCAAAGAGACTCATGGTGTACAGTCTAGGCGTGACTACGCGTGTCATCATTAGCAGCCACCCGGACACTGCTAAGCAAATCTTAGGAGGGTCTTCCTTTTCCGACCGTCCTATTAAGCAATCCGCTCGAGCACTAATGTTCAAGCGCGCCATAGGGTTTGCTCCTTCCGGGGCGTACTGGCGCCACCTGCGTAGGTTAGCAGCAGTCCACATGTTCTCGCCTAAAAGAATTGCCGGACTGGAAGGGCTAAGGCAGAACGTGGCGGACCAAATGGCGGTGAGGGTGCAGGAGGAGATGGAGGAGAGGAGATTTGTGTTGGTGAGAGGAATATTGCAGGTGGGTTCTATGAATAACATAATTGAGAGTGTTTTTGGGTTAGGGTTAGAGGATGAGGAGGGTAGTAGTTCTGACTTGACGTTGACTCTGCATGACATGGTCAAAGAAGGGTATGACTTAATTTCTAGGTTTAATTTGGAGGATTATATTCCTCTGAGGTTCTTGGACTTCTACGGTGTGAAGAGGAAGTGTCACAACTTGGCGGCTAAGGTTAAGAGTGTGGTGGGTGAAATTGTGAAGGAAAGAAAAAGAGGAGGAGATTTTAGTGGCCGGAGTGACTTTCTCAGTGCTCTGCTAGCTTTGCCTGTGGAGGATCAGTTGAGTGATTCAGACTTGGTTGCTGTTCTGTGGGAAATGATATTTCGAGGGACAGACACTGTTGCGATACTTCTTGAGTGGATTATGGCAAGGATTGCCTTGCACCAAGACATCCAAGCCAAGGCACAAGAAGAGCTTAATACAATCGTTGGGAATCACAGGAACGTGAGGGATTCCGACCTTCCGAAACTGCCGTACCTTCAAGCCATAGTTAAGGAAGTGCTCCGGATGCACCCTCCGGGCCCATTGCTATCGTGGGCCCGTCTCACGGTGCATGACGTCCACGTTGACAAGGTTCTTGTGCCTGCTGGCACAACGGCCATGGTCAACATGTGGGCTATAACCCATGACCCGGCCATCTGGAAGGACCCGTGGGTGTTTAAACCTGAGCGGTTCATTGAAGAGGACGTGTCGATAATGGGATCGGACCTTAGGCTTGCGCCCTTTGGCTCCGGCCGTAGGGCATGCCCGGGAAAAGCGCTAGGGTTAGCTACAGTGCACTTGTGGCTTGCAAGGCTTTTGCACCAGTTCAAGTGGCTTCCGGCGGTGCCCGTAGACCTTTCGGAGTGCTTAATGCTGTCTATGGAAATGAAGAAACCACTCACCTGCCGTGTTATCCCGCGTTGTGGCCTTACAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00232 ko00380 ko00591 ko00830 ko00980 ko00982 ko01100 ko01110 ko05204 map00140 map00232 map00380 map00591 map00830 map00980 map00982 map01100 map01110 map05204
Pfam Domains
Protein Families
p450

Protein Analysis

523

Amino Acids

58.51

Weight (kDa)

9.23

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 60 - 500 6.9e-79 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015603)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23820
malus_domestica MD13G1118500.v1.1 MD16G1118600.v1.1
prunus_persica Prupe.1G226500_v2.0.a1
pyrus_communis pycom13g10320 pycom16g10160
rosa_chinensis RchiOBHm_Chr4g0430371
rosa_laevigata RLG00000006981
rosa_multiflora Rmu_ssc0000082.1_g000001
rosa_roxburghii Rroxscaffold_5G00372170
rosa_rugosa Rorug04G0242700
rosa_samantha Rh4AG299100 Rh4BG305700 Rh4CG321600 Rh4DG303000
rosa_wichuraiana Rw4G025960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 468, 598, 1541
AatII GACGTC 1 cut(s) 1209
Acc36I ACCTGC 3 cut(s) 468, 598, 1541
AccB1I GGYRCC 2 cut(s) 453, 1474
AccBSI CCGCTC 2 cut(s) 398, 1318
AccI GTMKAC 2 cut(s) 244, 1482
AccII CGCG 3 cut(s) 313, 420, 1549
AccIII TCCGGA 1 cut(s) 1152
AciI CCGC 7 cut(s) 396, 536, 548, 812, 1318, 1472, 1547
AclWI GGATC 2 cut(s) 930, 1357
AcoI YGGCCR 5 cut(s) 160, 877, 1245, 1281, 1381
AcyI GRCGYC 2 cut(s) 454, 1206
AfaI GTAC 3 cut(s) 293, 449, 1124
AfeI AGCGCT 1 cut(s) 1407
AfiI CCNNNNNNNGG 7 cut(s) 203, 547, 1048, 1195, 1279, 1386, 1553
AflIII ACRYGT 4 cut(s) 311, 483, 1257, 1335
AgeI ACCGGT 1 cut(s) 184
AgsI TTSAA 4 cut(s) 415, 1130, 1328, 1459
AjiI CACGTC 1 cut(s) 1336
AluBI AGCT 7 cut(s) 53, 228, 263, 908, 1063, 1418, 1566
AluI AGCT 7 cut(s) 53, 228, 263, 908, 1063, 1418, 1566
Alw21I GWGCWC 5 cut(s) 230, 406, 901, 1152, 1428
Alw26I GTCTC 2 cut(s) 274, 1196
Alw44I GTGCAC 1 cut(s) 1424
AlwI GGATC 2 cut(s) 930, 1357
AlwNI CAGNNNCTG 1 cut(s) 986
Ama87I CYCGRG 3 cut(s) 215, 399, 1396
Aor13HI TCCGGA 1 cut(s) 1152
Aor51HI AGCGCT 1 cut(s) 1407
AoxI GGCC 9 cut(s) 160, 194, 877, 1168, 1185, 1245, 1281, 1381, 1555
ApaI GGGCCC 2 cut(s) 1172, 1189
ApaLI GTGCAC 1 cut(s) 1424
ApeKI GCWGC 3 cut(s) 260, 327, 473
AsiGI ACCGGT 1 cut(s) 184
AspLEI GCGC 5 cut(s) 420, 422, 456, 1369, 1408
AsuC2I CCSGG 7 cut(s) 135, 335, 442, 1167, 1280, 1397, 1398
AsuHPI GGTGA 4 cut(s) 562, 604, 848, 1522
AsuNHI GCTAGC 1 cut(s) 904
AvaI CYCGRG 3 cut(s) 215, 399, 1396
AvaII GGWCC 5 cut(s) 150, 182, 538, 1294, 1354
AxyI CCTNAGG 1 cut(s) 1358
BaeGI GKGCMC 4 cut(s) 1172, 1189, 1428, 1479
BaeI ACNNNNGTAYC 2 cut(s) 1106, 1139
BanI GGYRCC 2 cut(s) 453, 1474
BanII GRGCYC 3 cut(s) 230, 1172, 1189
BbsI GAAGAC 2 cut(s) 94, 357
Bbv12I GWGCWC 5 cut(s) 230, 406, 901, 1152, 1428
BbvI GCAGC 3 cut(s) 272, 339, 485
BccI CCATC 3 cut(s) 38, 562, 1292
BceAI ACGGC 5 cut(s) 175, 1105, 1260, 1368, 1521
BcgI CGANNNNNNTGC 2 cut(s) 380, 414
BcnI CCSGG 7 cut(s) 135, 335, 442, 1167, 1280, 1397, 1398
BcoDI GTCTC 2 cut(s) 274, 1196
BfaI CTAG 5 cut(s) 248, 299, 735, 905, 1409
BfmI CTRYAG 1 cut(s) 1419
BfoI RGCGCY 2 cut(s) 457, 1409
BfuAI ACCTGC 3 cut(s) 468, 598, 1541
BglI GCCNNNNNGGC 1 cut(s) 257
BisI GCNGC 4 cut(s) 261, 328, 474, 813
BlpI GCTNAGC 1 cut(s) 345
BlsI GCNGC 4 cut(s) 262, 329, 475, 814
Bme1390I CCNGG 7 cut(s) 135, 335, 442, 1167, 1280, 1397, 1398
Bme18I GGWCC 5 cut(s) 150, 182, 538, 1294, 1354
BmeT110I CYCGRG 3 cut(s) 215, 399, 1396
BmgBI CACGTC 1 cut(s) 1336
BmiI GGNNCC 7 cut(s) 208, 455, 1170, 1187, 1296, 1378, 1476
BmrFI CCNGG 7 cut(s) 135, 335, 442, 1167, 1280, 1397, 1398
BmsI GCATC 2 cut(s) 262, 1146
BmtI GCTAGC 1 cut(s) 908
BpiI GAAGAC 2 cut(s) 94, 357
Bpu10I CCTNAGC 3 cut(s) 521, 816, 1314
Bpu1102I GCTNAGC 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 1022
BpuMI CCSGG 7 cut(s) 135, 335, 442, 1167, 1280, 1397, 1398
BsaHI GRCGYC 2 cut(s) 454, 1206
BsaJI CCNNGG 5 cut(s) 441, 1047, 1248, 1298, 1396
BsaWI WCCGGW 3 cut(s) 152, 184, 1152
BsaXI ACNNNNNCTCC 2 cut(s) 911, 941
Bsc4I CCNNNNNNNGG 7 cut(s) 203, 547, 1048, 1195, 1279, 1386, 1553
Bse118I RCCGGY 1 cut(s) 184
Bse1I ACTGG 3 cut(s) 455, 516, 1453
Bse21I CCTNAGG 1 cut(s) 1358
Bse3DI GCAATG 1 cut(s) 1172
BseAI TCCGGA 1 cut(s) 1152
BseDI CCNNGG 5 cut(s) 441, 1047, 1248, 1298, 1396
BseGI GGATG 5 cut(s) 30, 277, 667, 1038, 1161
BseLI CCNNNNNNNGG 7 cut(s) 203, 547, 1048, 1195, 1279, 1386, 1553
BseMI GCAATG 1 cut(s) 1172
BseMII CTCAG 3 cut(s) 755, 907, 1305
BseNI ACTGG 3 cut(s) 455, 516, 1453
BsePI GCGCGC 1 cut(s) 418
BseRI GAGGAG 6 cut(s) 578, 587, 592, 680, 876, 879
BseSI GKGCMC 4 cut(s) 1172, 1189, 1428, 1479
BseX3I CGGCCG 1 cut(s) 1381
BseXI GCAGC 3 cut(s) 272, 339, 485
BsgI GTGCAG 1 cut(s) 578
Bsh1236I CGCG 3 cut(s) 313, 420, 1549
Bsh1285I CGRYCG 2 cut(s) 379, 1384
BshFI GGCC 9 cut(s) 162, 196, 879, 1170, 1187, 1247, 1283, 1383, 1557
BshNI GGYRCC 2 cut(s) 453, 1474
BshTI ACCGGT 1 cut(s) 184
BsiEI CGRYCG 2 cut(s) 379, 1384
BsiHKAI GWGCWC 5 cut(s) 230, 406, 901, 1152, 1428
BsiHKCI CYCGRG 3 cut(s) 215, 399, 1396
BslFI GGGAC 1 cut(s) 991
BslI CCNNNNNNNGG 7 cut(s) 203, 547, 1048, 1195, 1279, 1386, 1553
BsmAI GTCTC 2 cut(s) 274, 1196
BsmBI CGTCTC 1 cut(s) 1196
BsmFI GGGAC 1 cut(s) 991
BsnI GGCC 9 cut(s) 162, 196, 879, 1170, 1187, 1247, 1283, 1383, 1557
BsoBI CYCGRG 3 cut(s) 215, 399, 1396
Bsp120I GGGCCC 2 cut(s) 1168, 1185
Bsp1286I GDGCHC 8 cut(s) 230, 406, 901, 1152, 1172, 1189, 1428, 1479
Bsp13I TCCGGA 1 cut(s) 1152
Bsp1407I TGTACA 1 cut(s) 291
Bsp143I GATC 2 cut(s) 922, 1349
Bsp1720I GCTNAGC 1 cut(s) 345
Bsp19I CCATGG 1 cut(s) 1248
BspACI CCGC 7 cut(s) 396, 536, 548, 812, 1318, 1472, 1547
BspANI GGCC 9 cut(s) 162, 196, 879, 1170, 1187, 1247, 1283, 1383, 1557
BspCNI CTCAG 3 cut(s) 756, 906, 1306
BspEI TCCGGA 1 cut(s) 1152
BspFNI CGCG 3 cut(s) 313, 420, 1549
BspLI GGNNCC 7 cut(s) 208, 455, 1170, 1187, 1296, 1378, 1476
BspMI ACCTGC 3 cut(s) 468, 598, 1541
BspOI GCTAGC 1 cut(s) 908
BspPI GGATC 2 cut(s) 930, 1357
BspQI GCTCTTC 1 cut(s) 1053
BspT107I GGYRCC 2 cut(s) 453, 1474
BsrBI CCGCTC 2 cut(s) 398, 1318
BsrDI GCAATG 1 cut(s) 1172
BsrFI RCCGGY 1 cut(s) 184
BsrGI TGTACA 1 cut(s) 291
BsrI ACTGG 3 cut(s) 455, 516, 1453
BssAI RCCGGY 1 cut(s) 184
BssECI CCNNGG 5 cut(s) 441, 1047, 1248, 1298, 1396
BssHII GCGCGC 1 cut(s) 418
BssMI GATC 2 cut(s) 922, 1349
BssNI GRCGYC 2 cut(s) 454, 1206
BssT1I CCWWGG 2 cut(s) 1047, 1248
Bst4CI ACNGT 6 cut(s) 296, 380, 785, 988, 1198, 1423
Bst6I CTCTTC 5 cut(s) 14, 113, 785, 1053, 1323
BstACI GRCGYC 2 cut(s) 454, 1206
BstAPI GCANNNNNTGC 1 cut(s) 1022
BstAUI TGTACA 1 cut(s) 291
BstC8I GCNNGC 8 cut(s) 253, 420, 906, 1233, 1237, 1365, 1393, 1437
BstDEI CTNAG 8 cut(s) 345, 355, 521, 764, 816, 893, 1314, 1358
BstDSI CCRYGG 2 cut(s) 1248, 1298
BstF5I GGATG 5 cut(s) 30, 277, 667, 1038, 1161
BstFNI CGCG 3 cut(s) 313, 420, 1549
BstH2I RGCGCY 2 cut(s) 457, 1409
BstHHI GCGC 5 cut(s) 420, 422, 456, 1369, 1408
BstKTI GATC 2 cut(s) 925, 1352
BstMAI GTCTC 2 cut(s) 274, 1196
BstMBI GATC 2 cut(s) 922, 1349
BstMCI CGRYCG 2 cut(s) 379, 1384
BstMWI GCNNNNNNNGC 9 cut(s) 257, 460, 905, 910, 1022, 1156, 1432, 1441, 1563
BstNSI RCATGY 3 cut(s) 487, 1261, 1395
BstSCI CCNGG 7 cut(s) 133, 333, 440, 1165, 1278, 1395, 1396
BstSFI CTRYAG 1 cut(s) 1419
BstSLI GKGCMC 4 cut(s) 1172, 1189, 1428, 1479
BstUI CGCG 3 cut(s) 313, 420, 1549
BstV1I GCAGC 3 cut(s) 272, 339, 485
BstV2I GAAGAC 2 cut(s) 94, 357
BstZI CGGCCG 1 cut(s) 1381
Bsu36I CCTNAGG 1 cut(s) 1358
BsuRI GGCC 9 cut(s) 162, 196, 879, 1170, 1187, 1247, 1283, 1383, 1557
BtgI CCRYGG 2 cut(s) 1248, 1298
BtrI CACGTC 1 cut(s) 1336
BtsCI GGATG 5 cut(s) 30, 277, 667, 1038, 1161
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 4 cut(s) 339, 901, 984, 1428
BveI ACCTGC 3 cut(s) 468, 598, 1541
Cac8I GCNNGC 8 cut(s) 253, 420, 906, 1233, 1237, 1365, 1393, 1437
CaiI CAGNNNCTG 1 cut(s) 986
CfoI GCGC 5 cut(s) 420, 422, 456, 1369, 1408
Cfr10I RCCGGY 1 cut(s) 184
Cfr9I CCCGGG 1 cut(s) 1396
Csp6I GTAC 3 cut(s) 292, 448, 1123
CspAI ACCGGT 1 cut(s) 184
CviQI GTAC 3 cut(s) 292, 448, 1123
DdeI CTNAG 8 cut(s) 345, 355, 521, 764, 816, 893, 1314, 1358
DinI GGCGCC 1 cut(s) 455
DpnI GATC 2 cut(s) 924, 1351
DpnII GATC 2 cut(s) 922, 1349
DraI TTTAAA 1 cut(s) 1309
EaeI YGGCCR 5 cut(s) 160, 877, 1245, 1281, 1381
EagI CGGCCG 1 cut(s) 1381
Eam1104I CTCTTC 5 cut(s) 14, 113, 785, 1053, 1323
EarI CTCTTC 5 cut(s) 14, 113, 785, 1053, 1323
EciI GGCGGA 1 cut(s) 551
Ecl136II GAGCTC 1 cut(s) 228
EclXI CGGCCG 1 cut(s) 1381
Eco130I CCWWGG 2 cut(s) 1047, 1248
Eco24I GRGCYC 3 cut(s) 230, 1172, 1189
Eco47I GGWCC 5 cut(s) 150, 182, 538, 1294, 1354
Eco47III AGCGCT 1 cut(s) 1407
Eco52I CGGCCG 1 cut(s) 1381
Eco53kI GAGCTC 1 cut(s) 228
Eco81I CCTNAGG 1 cut(s) 1358
Eco88I CYCGRG 3 cut(s) 215, 399, 1396
EcoICRI GAGCTC 1 cut(s) 228
EcoO109I RGGNCCY 1 cut(s) 1294
EcoT14I CCWWGG 2 cut(s) 1047, 1248
EcoT38I GRGCYC 3 cut(s) 230, 1172, 1189
EgeI GGCGCC 1 cut(s) 455
EheI GGCGCC 1 cut(s) 455
ErhI CCWWGG 2 cut(s) 1047, 1248
Esp3I CGTCTC 1 cut(s) 1196
FalI AAGNNNNNCTT 4 cut(s) 338, 370, 1047, 1079
FaqI GGGAC 1 cut(s) 991
FauI CCCGC 1 cut(s) 1554
FblI GTMKAC 2 cut(s) 244, 1482
Fnu4HI GCNGC 4 cut(s) 261, 328, 474, 813
FokI GGATG 5 cut(s) 17, 284, 674, 1025, 1168
FriOI GRGCYC 3 cut(s) 230, 1172, 1189
Fsp4HI GCNGC 4 cut(s) 261, 328, 474, 813
FspBI CTAG 5 cut(s) 248, 299, 735, 905, 1409
GlaI GCGC 5 cut(s) 419, 421, 455, 1368, 1407
GluI GCNGC 4 cut(s) 261, 328, 474, 813
HaeII RGCGCY 2 cut(s) 457, 1409
HaeIII GGCC 9 cut(s) 162, 196, 879, 1170, 1187, 1247, 1283, 1383, 1557
HhaI GCGC 5 cut(s) 420, 422, 456, 1369, 1408
Hin1I GRCGYC 2 cut(s) 454, 1206
Hin6I GCGC 5 cut(s) 418, 420, 454, 1367, 1406
HinP1I GCGC 5 cut(s) 418, 420, 454, 1367, 1406
HincII GTYRAC 3 cut(s) 693, 1216, 1255
HindII GTYRAC 3 cut(s) 693, 1216, 1255
HinfI GANTC 6 cut(s) 6, 282, 694, 935, 1081, 1100
HphI GGTGA 4 cut(s) 562, 604, 848, 1522
Hpy166II GTNNAC 9 cut(s) 245, 292, 481, 693, 1210, 1216, 1255, 1426, 1483
Hpy188I TCNGA 8 cut(s) 376, 682, 765, 940, 1105, 1113, 1354, 1492
Hpy188III TCNNGA 3 cut(s) 1001, 1153, 1288
Hpy8I GTNNAC 9 cut(s) 245, 292, 481, 693, 1210, 1216, 1255, 1426, 1483
HpyAV CCTTC 7 cut(s) 447, 509, 710, 841, 1118, 1136, 1285
HpyCH4III ACNGT 6 cut(s) 296, 380, 785, 988, 1198, 1423
HpyCH4IV ACGT 6 cut(s) 531, 689, 1092, 1206, 1212, 1335
HpyF10VI GCNNNNNNNGC 9 cut(s) 257, 460, 905, 910, 1022, 1156, 1432, 1441, 1563
HpyF3I CTNAG 8 cut(s) 345, 355, 521, 764, 816, 893, 1314, 1358
HpySE526I ACGT 6 cut(s) 531, 689, 1092, 1206, 1212, 1335
Hsp92I GRCGYC 2 cut(s) 454, 1206
HspAI GCGC 5 cut(s) 418, 420, 454, 1367, 1406
KasI GGCGCC 1 cut(s) 453
Kpn2I TCCGGA 1 cut(s) 1152
Kzo9I GATC 2 cut(s) 922, 1349
LguI GCTCTTC 1 cut(s) 1053
LmnI GCTCC 4 cut(s) 50, 439, 1155, 1382
Lsp1109I GCAGC 3 cut(s) 272, 339, 485
LweI GCATC 2 cut(s) 262, 1146
MaeI CTAG 5 cut(s) 248, 299, 735, 905, 1409
MaeII ACGT 6 cut(s) 531, 689, 1092, 1206, 1212, 1335
MaeIII GTNAC 6 cut(s) 112, 119, 155, 304, 800, 884
MalI GATC 2 cut(s) 924, 1351
MbiI CCGCTC 2 cut(s) 398, 1318
MboI GATC 2 cut(s) 922, 1349
MboII GAAGA 8 cut(s) 85, 94, 100, 357, 802, 1070, 1340, 1531
MhlI GDGCHC 8 cut(s) 230, 406, 901, 1152, 1172, 1189, 1428, 1479
MluCI AATT 5 cut(s) 501, 630, 729, 742, 840
MluI ACGCGT 1 cut(s) 311
Mly113I GGCGCC 1 cut(s) 454
MlyI GAGTC 2 cut(s) 276, 688
MmeI TCCRAC 3 cut(s) 249, 399, 1128
MroI TCCGGA 1 cut(s) 1152
MseI TTAA 8 cut(s) 387, 728, 741, 822, 1065, 1140, 1308, 1499
MspR9I CCNGG 7 cut(s) 135, 335, 442, 1167, 1280, 1397, 1398
MssI GTTTAAAC 1 cut(s) 1309
MvnI CGCG 3 cut(s) 313, 420, 1549
MwoI GCNNNNNNNGC 9 cut(s) 257, 460, 905, 910, 1022, 1156, 1432, 1441, 1563
NarI GGCGCC 1 cut(s) 454
NciI CCSGG 7 cut(s) 135, 335, 442, 1167, 1280, 1397, 1398
NcoI CCATGG 1 cut(s) 1248
NdeII GATC 2 cut(s) 922, 1349
NheI GCTAGC 1 cut(s) 904
NlaIV GGNNCC 7 cut(s) 208, 455, 1170, 1187, 1296, 1378, 1476
NmuCI GTSAC 3 cut(s) 304, 800, 884
NspI RCATGY 3 cut(s) 487, 1261, 1395
PaeI GCATGC 1 cut(s) 1395
PaeR7I CTCGAG 1 cut(s) 399
PaqCI CACCTGC 3 cut(s) 468, 598, 1541
PauI GCGCGC 1 cut(s) 418
PciI ACATGT 2 cut(s) 483, 1257
PciSI GCTCTTC 1 cut(s) 1053
PfeI GAWTC 3 cut(s) 935, 1081, 1100
PflFI GACNNNGTC 1 cut(s) 707
PfoI TCCNGGA 1 cut(s) 133
PinAI ACCGGT 1 cut(s) 184
PkrI GCNGC 4 cut(s) 262, 329, 475, 814
PleI GAGTC 2 cut(s) 276, 688
PluTI GGCGCC 1 cut(s) 457
PmeI GTTTAAAC 1 cut(s) 1309
PpsI GAGTC 2 cut(s) 276, 688
PpuMI RGGWCCY 1 cut(s) 1294
PscI ACATGT 2 cut(s) 483, 1257
Psp124BI GAGCTC 1 cut(s) 230
Psp5II RGGWCCY 1 cut(s) 1294
PspN4I GGNNCC 7 cut(s) 208, 455, 1170, 1187, 1296, 1378, 1476
PspOMI GGGCCC 2 cut(s) 1168, 1185
PspPPI RGGWCCY 1 cut(s) 1294
PspXI VCTCGAGB 1 cut(s) 399
PstNI CAGNNNCTG 1 cut(s) 986
PsyI GACNNNGTC 1 cut(s) 707
PteI GCGCGC 1 cut(s) 418
RsaI GTAC 3 cut(s) 293, 449, 1124
RsaNI GTAC 3 cut(s) 292, 448, 1123
SacI GAGCTC 1 cut(s) 230
SapI GCTCTTC 1 cut(s) 1053
SaqAI TTAA 8 cut(s) 387, 728, 741, 822, 1065, 1140, 1308, 1499
SatI GCNGC 4 cut(s) 261, 328, 474, 813
Sau3AI GATC 2 cut(s) 922, 1349
SchI GAGTC 2 cut(s) 276, 688
ScrFI CCNGG 7 cut(s) 135, 335, 442, 1167, 1280, 1397, 1398
SduI GDGCHC 8 cut(s) 230, 406, 901, 1152, 1172, 1189, 1428, 1479
SfaNI GCATC 2 cut(s) 262, 1146
SfcI CTRYAG 1 cut(s) 1419
SfoI GGCGCC 1 cut(s) 455
Sfr274I CTCGAG 1 cut(s) 399
SinI GGWCC 5 cut(s) 150, 182, 538, 1294, 1354
SlaI CTCGAG 1 cut(s) 399
SmaI CCCGGG 1 cut(s) 1398
SmlI CTYRAG 2 cut(s) 399, 1001
SmoI CTYRAG 2 cut(s) 399, 1001
SphI GCATGC 1 cut(s) 1395
Sse9I AATT 5 cut(s) 501, 630, 729, 742, 840
SsiI CCGC 7 cut(s) 396, 536, 548, 812, 1318, 1472, 1547
SspDI GGCGCC 1 cut(s) 453
SspI AATATT 1 cut(s) 603
SspMI CTAG 5 cut(s) 248, 299, 735, 905, 1409
SstI GAGCTC 1 cut(s) 230
StyD4I CCNGG 7 cut(s) 133, 333, 440, 1165, 1278, 1395, 1396
StyI CCWWGG 2 cut(s) 1047, 1248
TaaI ACNGT 6 cut(s) 296, 380, 785, 988, 1198, 1423
TaiI ACGT 6 cut(s) 534, 692, 1095, 1209, 1215, 1338
TaqI TCGA 4 cut(s) 178, 400, 973, 1340
TasI AATT 5 cut(s) 501, 630, 729, 742, 840
TatI WGTACW 1 cut(s) 291
TauI GCSGC 1 cut(s) 815
TfiI GAWTC 3 cut(s) 935, 1081, 1100
Tru1I TTAA 8 cut(s) 387, 728, 741, 822, 1065, 1140, 1308, 1499
Tru9I TTAA 8 cut(s) 387, 728, 741, 822, 1065, 1140, 1308, 1499
TscAI CASTG 4 cut(s) 346, 901, 991, 1428
TseFI GTSAC 3 cut(s) 304, 800, 884
TseI GCWGC 3 cut(s) 260, 327, 473
Tsp45I GTSAC 3 cut(s) 304, 800, 884
TspDTI ATGAA 5 cut(s) 75, 185, 635, 1312, 1532
TspMI CCCGGG 1 cut(s) 1396
TspRI CASTG 4 cut(s) 346, 901, 991, 1428
Tth111I GACNNNGTC 1 cut(s) 707
VneI GTGCAC 1 cut(s) 1424
VpaK11BI GGWCC 5 cut(s) 150, 182, 538, 1294, 1354
XceI RCATGY 3 cut(s) 487, 1261, 1395
XcmI CCANNNNNNNNNTGG 1 cut(s) 1179
XhoI CTCGAG 1 cut(s) 399
XmaI CCCGGG 1 cut(s) 1396
XmiI GTMKAC 2 cut(s) 244, 1482
XspI CTAG 5 cut(s) 248, 299, 735, 905, 1409
ZraI GACGTC 1 cut(s) 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.