Rh4DG303000

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0015603
Physical Location & Seq
Reverse (-)
52513703 .. 52516569
2867 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG303000.1

Sequence Viewer

Length: 1560 bp
ATGAAACTCTTCATAATAGCCAACCTCTCCATCCTACTCTTTTGGTTTGGAGCTTCTACCCTCACTCTCACTCTCCTCTCATTCTTCTTCTATGTCTTCCTCTTCGTCCTAAACCACTACCTCGTCCCCGGAGGCTCTGCATGGTCAGCTTACCGCCATGCATTGAAACTCCAGGGACCAGTGGGGTGGCCTGTATTGGGTTCCATATCCGACCAAATGAGCGCTCTTGCTCATCGTAAACTGGCCGGCATGGCAGCTTCGCTGGATGCAAAGAGACTCATGGTGTACAGTCTAGGAACCAGTACACGTGTCATCATTAGCAGCCACCCGGAGACTGCTCAGCAAATCCTAGGAGGGTCTTCCTTTTCCGACCGTCCTATTAAGCAATCCGCTCGAGCACTAATGTTCGAGCGCGCTATAGGGTTTGCTCCTTCAGGGGCGTACTGGCGCCACCTGCGTAGGTTAGCTGCAGTCCACATGTTCTCGCCGAAGAGAATTGCCGGACTGGAAGGACTAAGGCAGCACGTGGTGGACAGAATGGCTGTGAGAGTGGAGGATGAGATGGAGGAGAGGAGATTTGTGGGTGTGAAAGGAATATTGCAGGAGGGTTCCCTGAATAACATAATTGAGAGTGTTTTTGGGTTAGGGTTAGAGAAGGAGGGTAGTACTGACTTGACGTTGACTTTGCATGATATGGTCAAAGAAGGGTATGACTTAATTTCTAGGTTTAATTTGGAGGATTATTTTCCTCTGAGGTTCTTGGACTTCCATGGTGTGAAGAGGAAGTGTCACAAATTGGCGGCTAAGGTTAAGTGTGTGGTGGGTCAAATTGTGGAGGAAAGAAAAAGAGCAGGAGATTTCAGTGGTCGGAGTGACTTTCTTAGTGCTTTGCTAGCTTTGTCAGTGGAGGATCAATTAAGTGATGCAGACTTGGTTGCTGTTTTGTGGGAAATGATATTTCGAGGGACAGACACTGTTGCTATACTTCTTGAATGGATTATGGCAAGGATTGCCCTGCACCAAGACATCCAAGCCAAGGCACAAGAAGAGCTTGATACGATCGTTGGGAATCACAGGCACGTGAGGGATTCTGACCTTTCGAAACTGCCGTATCTTCTGGCCATAGTTAAGGAAGTGCTCCGGGTGCACCCTCCGGGCCCACTATTATCGTGGGCTCGTCTGGCCGTGCATGACGTCCACGTCGACAAGGTCTTTGTTCCTGCCGGCACAACGGCCATGGTCAACATGTGGGCCATAACCCATGACCCGGCCATTTGGAAGGACCCTTGGACTTTCAAGCCTGAGCGGTTCATTGAAGAAGATGTGTCGATAATAGGATCGGACCTTAGGCTTGCGCCCTTTGGTTCGGGCCGTAGGGTATGCCCGGGAAAAGCTCTAGGGTTAGCTACAGTGCACTTGTGGCTTGCAAGGCTTTTGCACCAATTCAAGTGGTTTCCGGCGGAGCCTGTAGACCTTTCGGAGTGCTTAATGCTCTCTATGGAAATGAAGAAACCACTCGTCTGCCGCGTTATCCCGCGTGGTGGCCTTACAAGCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00232 ko00380 ko00591 ko00830 ko00980 ko00982 ko01100 ko01110 ko05204 map00140 map00232 map00380 map00591 map00830 map00980 map00982 map01100 map01110 map05204
Pfam Domains
Protein Families
p450

Protein Analysis

519

Amino Acids

58.18

Weight (kDa)

8.94

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 59 - 503 4.3e-77 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015603)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23820
malus_domestica MD13G1118500.v1.1 MD16G1118600.v1.1
prunus_persica Prupe.1G226500_v2.0.a1
pyrus_communis pycom13g10320 pycom16g10160
rosa_chinensis RchiOBHm_Chr4g0430371
rosa_laevigata RLG00000006981
rosa_multiflora Rmu_ssc0000082.1_g000001
rosa_roxburghii Rroxscaffold_5G00372170
rosa_rugosa Rorug04G0242700
rosa_samantha Rh4AG299100 Rh4BG305700 Rh4CG321600 Rh4DG303000
rosa_wichuraiana Rw4G025960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 462
AasI GACNNNNNNGTC 1 cut(s) 1199
AatII GACGTC 1 cut(s) 1197
Acc36I ACCTGC 1 cut(s) 462
AccB1I GGYRCC 1 cut(s) 447
AccBSI CCGCTC 2 cut(s) 392, 1306
AccI GTMKAC 2 cut(s) 1203, 1470
AccII CGCG 3 cut(s) 414, 1527, 1537
AciI CCGC 7 cut(s) 154, 390, 800, 1306, 1460, 1525, 1535
AclWI GGATC 2 cut(s) 918, 1345
AcoI YGGCCR 5 cut(s) 243, 1119, 1182, 1233, 1269
AcuI CTGAAG 1 cut(s) 417
AcvI CACGTG 3 cut(s) 308, 526, 1081
AcyI GRCGYC 2 cut(s) 448, 1194
AdeI CACNNNGTG 1 cut(s) 529
AfaI GTAC 4 cut(s) 287, 304, 443, 667
AfeI AGCGCT 1 cut(s) 223
AfiI CCNNNNNNNGG 4 cut(s) 197, 1036, 1267, 1541
AflIII ACRYGT 4 cut(s) 305, 307, 477, 1245
AgsI TTSAA 5 cut(s) 166, 992, 1297, 1316, 1447
AjiI CACGTC 1 cut(s) 1201
AjnI CCWGG 1 cut(s) 171
AluBI AGCT 8 cut(s) 53, 149, 257, 467, 896, 1051, 1394, 1406
AluI AGCT 8 cut(s) 53, 149, 257, 467, 896, 1051, 1394, 1406
Alw21I GWGCWC 4 cut(s) 400, 1140, 1149, 1416
Alw26I GTCTC 2 cut(s) 268, 326
Alw44I GTGCAC 2 cut(s) 1145, 1412
AlwI GGATC 2 cut(s) 918, 1345
AlwNI CAGNNNCTG 1 cut(s) 974
Ama87I CYCGRG 2 cut(s) 393, 1384
Aor51HI AGCGCT 1 cut(s) 223
ApaI GGGCCC 1 cut(s) 1160
ApaLI GTGCAC 2 cut(s) 1145, 1412
ApeKI GCWGC 4 cut(s) 254, 321, 467, 520
ArsI GACNNNNNNTTYG 4 cut(s) 667, 699, 1196, 1228
Asp700I GAANNNNTTC 1 cut(s) 8
AspA2I CCTAGG 1 cut(s) 349
AspLEI GCGC 5 cut(s) 224, 414, 416, 450, 1357
AspS9I GGNCC 7 cut(s) 176, 1156, 1157, 1251, 1282, 1342, 1369
AsuC2I CCSGG 7 cut(s) 129, 329, 1142, 1155, 1268, 1385, 1386
AsuII TTCGAA 1 cut(s) 1100
AsuNHI GCTAGC 1 cut(s) 892
AvaI CYCGRG 2 cut(s) 393, 1384
AvaII GGWCC 3 cut(s) 176, 1282, 1342
AvrII CCTAGG 1 cut(s) 349
AxyI CCTNAGG 1 cut(s) 1346
BaeGI GKGCMC 3 cut(s) 1149, 1160, 1416
BaeI ACNNNNGTAYC 4 cut(s) 1047, 1080, 1094, 1127
BalI TGGCCA 1 cut(s) 1121
BanI GGYRCC 1 cut(s) 447
BanII GRGCYC 2 cut(s) 1160, 1177
BbrPI CACGTG 3 cut(s) 308, 526, 1081
BbsI GAAGAC 2 cut(s) 88, 351
Bbv12I GWGCWC 4 cut(s) 400, 1140, 1149, 1416
BbvI GCAGC 4 cut(s) 266, 333, 454, 532
BccI CCATC 2 cut(s) 38, 556
BceAI ACGGC 4 cut(s) 1093, 1169, 1248, 1356
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BciT130I CCWGG 1 cut(s) 173
BcnI CCSGG 7 cut(s) 129, 329, 1142, 1155, 1268, 1385, 1386
BcoDI GTCTC 2 cut(s) 268, 326
BfaI CTAG 5 cut(s) 293, 350, 723, 893, 1397
BfmI CTRYAG 4 cut(s) 417, 468, 1407, 1467
BfoI RGCGCY 2 cut(s) 225, 451
BfuAI ACCTGC 1 cut(s) 462
BglI GCCNNNNNGGC 1 cut(s) 251
BisI GCNGC 6 cut(s) 255, 322, 468, 521, 801, 1525
BlnI CCTAGG 1 cut(s) 349
BlpI GCTNAGC 1 cut(s) 339
BlsI GCNGC 6 cut(s) 256, 323, 469, 522, 802, 1526
BmcAI AGTACT 1 cut(s) 667
Bme1390I CCNGG 8 cut(s) 129, 173, 329, 1142, 1155, 1268, 1385, 1386
Bme18I GGWCC 3 cut(s) 176, 1282, 1342
BmeT110I CYCGRG 2 cut(s) 393, 1384
BmgBI CACGTC 1 cut(s) 1201
BmgT120I GGNCC 7 cut(s) 176, 1156, 1157, 1251, 1282, 1342, 1369
BmiI GGNNCC 8 cut(s) 177, 202, 298, 449, 610, 1158, 1284, 1464
BmrFI CCNGG 8 cut(s) 129, 173, 329, 1142, 1155, 1268, 1385, 1386
BmsI GCATC 2 cut(s) 256, 913
BmtI GCTAGC 1 cut(s) 896
BpiI GAAGAC 2 cut(s) 88, 351
BpmI CTGGAG 1 cut(s) 155
Bpu10I CCTNAGC 2 cut(s) 804, 1302
Bpu1102I GCTNAGC 1 cut(s) 339
Bpu14I TTCGAA 1 cut(s) 1100
BpuMI CCSGG 7 cut(s) 129, 329, 1142, 1155, 1268, 1385, 1386
BsaAI YACGTR 3 cut(s) 308, 526, 1081
BsaHI GRCGYC 2 cut(s) 448, 1194
BsaJI CCNNGG 8 cut(s) 127, 172, 349, 769, 1035, 1236, 1286, 1384
Bsc4I CCNNNNNNNGG 4 cut(s) 197, 1036, 1267, 1541
Bse118I RCCGGY 2 cut(s) 245, 1223
Bse1I ACTGG 5 cut(s) 179, 246, 300, 449, 510
Bse21I CCTNAGG 1 cut(s) 1346
BseBI CCWGG 1 cut(s) 173
BseDI CCNNGG 8 cut(s) 127, 172, 349, 769, 1035, 1236, 1286, 1384
BseGI GGATG 4 cut(s) 30, 271, 562, 1026
BseLI CCNNNNNNNGG 4 cut(s) 197, 1036, 1267, 1541
BseMII CTCAG 3 cut(s) 353, 743, 1293
BseNI ACTGG 5 cut(s) 179, 246, 300, 449, 510
BsePI GCGCGC 1 cut(s) 412
BseRI GAGGAG 3 cut(s) 65, 581, 586
BseSI GKGCMC 3 cut(s) 1149, 1160, 1416
BseXI GCAGC 4 cut(s) 266, 333, 454, 532
BsgI GTGCAG 1 cut(s) 1001
Bsh1236I CGCG 3 cut(s) 414, 1527, 1537
Bsh1285I CGRYCG 2 cut(s) 373, 1062
BshNI GGYRCC 1 cut(s) 447
BsiEI CGRYCG 2 cut(s) 373, 1062
BsiHKAI GWGCWC 4 cut(s) 400, 1140, 1149, 1416
BsiHKCI CYCGRG 2 cut(s) 393, 1384
BslFI GGGAC 3 cut(s) 110, 189, 979
BslI CCNNNNNNNGG 4 cut(s) 197, 1036, 1267, 1541
BsmAI GTCTC 2 cut(s) 268, 326
BsmFI GGGAC 3 cut(s) 110, 189, 979
BsoBI CYCGRG 2 cut(s) 393, 1384
Bsp119I TTCGAA 1 cut(s) 1100
Bsp120I GGGCCC 1 cut(s) 1156
Bsp1286I GDGCHC 6 cut(s) 400, 1140, 1149, 1160, 1177, 1416
Bsp1407I TGTACA 1 cut(s) 285
Bsp143I GATC 3 cut(s) 910, 1059, 1337
Bsp1720I GCTNAGC 1 cut(s) 339
Bsp19I CCATGG 2 cut(s) 769, 1236
BspACI CCGC 7 cut(s) 154, 390, 800, 1306, 1460, 1525, 1535
BspCNI CTCAG 3 cut(s) 352, 744, 1294
BspFNI CGCG 3 cut(s) 414, 1527, 1537
BspLI GGNNCC 8 cut(s) 177, 202, 298, 449, 610, 1158, 1284, 1464
BspMAI CTGCAG 1 cut(s) 472
BspMI ACCTGC 1 cut(s) 462
BspOI GCTAGC 1 cut(s) 896
BspPI GGATC 2 cut(s) 918, 1345
BspQI GCTCTTC 1 cut(s) 1041
BspT104I TTCGAA 1 cut(s) 1100
BspT107I GGYRCC 1 cut(s) 447
BsrBI CCGCTC 2 cut(s) 392, 1306
BsrFI RCCGGY 2 cut(s) 245, 1223
BsrGI TGTACA 1 cut(s) 285
BsrI ACTGG 5 cut(s) 179, 246, 300, 449, 510
BssAI RCCGGY 2 cut(s) 245, 1223
BssECI CCNNGG 8 cut(s) 127, 172, 349, 769, 1035, 1236, 1286, 1384
BssHII GCGCGC 1 cut(s) 412
BssMI GATC 3 cut(s) 910, 1059, 1337
BssNI GRCGYC 2 cut(s) 448, 1194
BssT1I CCWWGG 5 cut(s) 349, 769, 1035, 1236, 1286
Bst2UI CCWGG 1 cut(s) 173
Bst4CI ACNGT 4 cut(s) 290, 374, 976, 1411
Bst6I CTCTTC 5 cut(s) 14, 107, 485, 773, 1041
BstACI GRCGYC 2 cut(s) 448, 1194
BstAPI GCANNNNNTGC 1 cut(s) 1010
BstAUI TGTACA 1 cut(s) 285
BstBAI YACGTR 3 cut(s) 308, 526, 1081
BstBI TTCGAA 1 cut(s) 1100
BstC8I GCNNGC 6 cut(s) 247, 414, 894, 1225, 1353, 1425
BstDEI CTNAG 7 cut(s) 339, 515, 752, 804, 881, 1302, 1346
BstDSI CCRYGG 2 cut(s) 769, 1236
BstF5I GGATG 4 cut(s) 30, 271, 562, 1026
BstFNI CGCG 3 cut(s) 414, 1527, 1537
BstH2I RGCGCY 2 cut(s) 225, 451
BstHHI GCGC 5 cut(s) 224, 414, 416, 450, 1357
BstKTI GATC 3 cut(s) 913, 1062, 1340
BstMAI GTCTC 2 cut(s) 268, 326
BstMBI GATC 3 cut(s) 910, 1059, 1337
BstMCI CGRYCG 2 cut(s) 373, 1062
BstNI CCWGG 1 cut(s) 173
BstNSI RCATGY 2 cut(s) 481, 1249
BstSCI CCNGG 8 cut(s) 127, 171, 327, 1140, 1153, 1266, 1383, 1384
BstSFI CTRYAG 4 cut(s) 417, 468, 1407, 1467
BstSLI GKGCMC 3 cut(s) 1149, 1160, 1416
BstUI CGCG 3 cut(s) 414, 1527, 1537
BstV1I GCAGC 4 cut(s) 266, 333, 454, 532
BstV2I GAAGAC 2 cut(s) 88, 351
BstXI CCANNNNNNTGG 1 cut(s) 186
Bsu36I CCTNAGG 1 cut(s) 1346
BtgI CCRYGG 2 cut(s) 769, 1236
BtrI CACGTC 1 cut(s) 1201
BtsCI GGATG 4 cut(s) 30, 271, 562, 1026
BtsIMutI CAGTG 5 cut(s) 186, 868, 909, 972, 1416
BveI ACCTGC 1 cut(s) 462
Cac8I GCNNGC 6 cut(s) 247, 414, 894, 1225, 1353, 1425
CaiI CAGNNNCTG 1 cut(s) 974
CfoI GCGC 5 cut(s) 224, 414, 416, 450, 1357
Cfr10I RCCGGY 2 cut(s) 245, 1223
Cfr13I GGNCC 7 cut(s) 176, 1156, 1157, 1251, 1282, 1342, 1369
Cfr9I CCCGGG 1 cut(s) 1384
Csp6I GTAC 4 cut(s) 286, 303, 442, 666
CspCI CAANNNNNGTGG 2 cut(s) 1430, 1465
CviQI GTAC 4 cut(s) 286, 303, 442, 666
DdeI CTNAG 7 cut(s) 339, 515, 752, 804, 881, 1302, 1346
DinI GGCGCC 1 cut(s) 449
DpnI GATC 3 cut(s) 912, 1061, 1339
DpnII GATC 3 cut(s) 910, 1059, 1337
DraIII CACNNNGTG 1 cut(s) 529
DrdI GACNNNNNNGTC 1 cut(s) 1199
DseDI GACNNNNNNGTC 1 cut(s) 1199
EaeI YGGCCR 5 cut(s) 243, 1119, 1182, 1233, 1269
Eam1104I CTCTTC 5 cut(s) 14, 107, 485, 773, 1041
EarI CTCTTC 5 cut(s) 14, 107, 485, 773, 1041
EciI GGCGGA 1 cut(s) 1475
Eco130I CCWWGG 5 cut(s) 349, 769, 1035, 1236, 1286
Eco24I GRGCYC 2 cut(s) 1160, 1177
Eco47I GGWCC 3 cut(s) 176, 1282, 1342
Eco47III AGCGCT 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 417
Eco72I CACGTG 3 cut(s) 308, 526, 1081
Eco81I CCTNAGG 1 cut(s) 1346
Eco88I CYCGRG 2 cut(s) 393, 1384
EcoO109I RGGNCCY 1 cut(s) 1282
EcoRII CCWGG 1 cut(s) 171
EcoT14I CCWWGG 5 cut(s) 349, 769, 1035, 1236, 1286
EcoT22I ATGCAT 1 cut(s) 163
EcoT38I GRGCYC 2 cut(s) 1160, 1177
EgeI GGCGCC 1 cut(s) 449
EheI GGCGCC 1 cut(s) 449
ErhI CCWWGG 5 cut(s) 349, 769, 1035, 1236, 1286
FalI AAGNNNNNCTT 2 cut(s) 1035, 1067
FaqI GGGAC 3 cut(s) 110, 189, 979
FauI CCCGC 1 cut(s) 1542
FblI GTMKAC 2 cut(s) 1203, 1470
Fnu4HI GCNGC 6 cut(s) 255, 322, 468, 521, 801, 1525
FokI GGATG 4 cut(s) 17, 278, 569, 1013
FriOI GRGCYC 2 cut(s) 1160, 1177
Fsp4HI GCNGC 6 cut(s) 255, 322, 468, 521, 801, 1525
FspBI CTAG 5 cut(s) 293, 350, 723, 893, 1397
GlaI GCGC 5 cut(s) 223, 413, 415, 449, 1356
GluI GCNGC 6 cut(s) 255, 322, 468, 521, 801, 1525
GsuI CTGGAG 1 cut(s) 155
HaeII RGCGCY 2 cut(s) 225, 451
HhaI GCGC 5 cut(s) 224, 414, 416, 450, 1357
Hin1I GRCGYC 2 cut(s) 448, 1194
Hin6I GCGC 5 cut(s) 222, 412, 414, 448, 1355
HinP1I GCGC 5 cut(s) 222, 412, 414, 448, 1355
HincII GTYRAC 3 cut(s) 681, 1204, 1243
HindII GTYRAC 3 cut(s) 681, 1204, 1243
HinfI GANTC 3 cut(s) 276, 1069, 1088
Hpy188I TCNGA 7 cut(s) 211, 370, 753, 870, 1093, 1342, 1480
Hpy188III TCNNGA 1 cut(s) 989
Hpy99I CGWCG 1 cut(s) 1205
HpyAV CCTTC 5 cut(s) 441, 503, 649, 698, 1273
HpyCH4III ACNGT 4 cut(s) 290, 374, 976, 1411
HpyCH4IV ACGT 6 cut(s) 307, 525, 677, 1080, 1194, 1200
HpyF3I CTNAG 7 cut(s) 339, 515, 752, 804, 881, 1302, 1346
HpySE526I ACGT 6 cut(s) 307, 525, 677, 1080, 1194, 1200
Hsp92I GRCGYC 2 cut(s) 448, 1194
HspAI GCGC 5 cut(s) 222, 412, 414, 448, 1355
KasI GGCGCC 1 cut(s) 447
KroI GCCGGC 2 cut(s) 245, 1223
KroNI GCCGGC 2 cut(s) 247, 1225
Kzo9I GATC 3 cut(s) 910, 1059, 1337
LguI GCTCTTC 1 cut(s) 1041
LmnI GCTCC 4 cut(s) 50, 433, 1143, 1462
Lsp1109I GCAGC 4 cut(s) 266, 333, 454, 532
LweI GCATC 2 cut(s) 256, 913
MaeI CTAG 5 cut(s) 293, 350, 723, 893, 1397
MaeII ACGT 6 cut(s) 307, 525, 677, 1080, 1194, 1200
MaeIII GTNAC 2 cut(s) 788, 872
MalI GATC 3 cut(s) 912, 1061, 1339
MbiI CCGCTC 2 cut(s) 392, 1306
MboI GATC 3 cut(s) 910, 1059, 1337
MhlI GDGCHC 6 cut(s) 400, 1140, 1149, 1160, 1177, 1416
MlsI TGGCCA 1 cut(s) 1121
MluCI AATT 8 cut(s) 495, 624, 717, 730, 794, 828, 914, 1442
MluNI TGGCCA 1 cut(s) 1121
Mly113I GGCGCC 1 cut(s) 448
MlyI GAGTC 1 cut(s) 270
MmeI TCCRAC 3 cut(s) 234, 393, 848
Mox20I TGGCCA 1 cut(s) 1121
Mph1103I ATGCAT 1 cut(s) 163
MroNI GCCGGC 2 cut(s) 245, 1223
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 1121
MseI TTAA 7 cut(s) 381, 716, 729, 810, 917, 1128, 1487
Msp20I TGGCCA 1 cut(s) 1121
MspR9I CCNGG 8 cut(s) 129, 173, 329, 1142, 1155, 1268, 1385, 1386
MvaI CCWGG 1 cut(s) 173
MvnI CGCG 3 cut(s) 414, 1527, 1537
NaeI GCCGGC 2 cut(s) 247, 1225
NarI GGCGCC 1 cut(s) 448
NciI CCSGG 7 cut(s) 129, 329, 1142, 1155, 1268, 1385, 1386
NcoI CCATGG 2 cut(s) 769, 1236
NdeII GATC 3 cut(s) 910, 1059, 1337
NgoMIV GCCGGC 2 cut(s) 245, 1223
NheI GCTAGC 1 cut(s) 892
NlaIV GGNNCC 8 cut(s) 177, 202, 298, 449, 610, 1158, 1284, 1464
NmuCI GTSAC 2 cut(s) 788, 872
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 2 cut(s) 481, 1249
NspV TTCGAA 1 cut(s) 1100
PaeR7I CTCGAG 1 cut(s) 393
PaqCI CACCTGC 1 cut(s) 462
PauI GCGCGC 1 cut(s) 412
PciI ACATGT 2 cut(s) 477, 1245
PciSI GCTCTTC 1 cut(s) 1041
PcsI WCGNNNNNNNCGW 3 cut(s) 1106, 1200, 1524
PdiI GCCGGC 2 cut(s) 247, 1225
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 2 cut(s) 1069, 1088
PflFI GACNNNGTC 1 cut(s) 1208
PkrI GCNGC 6 cut(s) 256, 323, 469, 522, 802, 1526
Ple19I CGATCG 1 cut(s) 1062
PleI GAGTC 1 cut(s) 270
PluTI GGCGCC 1 cut(s) 451
PmaCI CACGTG 3 cut(s) 308, 526, 1081
PmlI CACGTG 3 cut(s) 308, 526, 1081
PpsI GAGTC 1 cut(s) 270
Ppu21I YACGTR 3 cut(s) 308, 526, 1081
PpuMI RGGWCCY 1 cut(s) 1282
PscI ACATGT 2 cut(s) 477, 1245
Psp5II RGGWCCY 1 cut(s) 1282
Psp6I CCWGG 1 cut(s) 171
PspCI CACGTG 3 cut(s) 308, 526, 1081
PspGI CCWGG 1 cut(s) 171
PspN4I GGNNCC 8 cut(s) 177, 202, 298, 449, 610, 1158, 1284, 1464
PspOMI GGGCCC 1 cut(s) 1156
PspPI GGNCC 7 cut(s) 176, 1156, 1157, 1251, 1282, 1342, 1369
PspPPI RGGWCCY 1 cut(s) 1282
PspXI VCTCGAGB 1 cut(s) 393
PstI CTGCAG 1 cut(s) 472
PstNI CAGNNNCTG 1 cut(s) 974
PsyI GACNNNGTC 1 cut(s) 1208
PteI GCGCGC 1 cut(s) 412
PvuI CGATCG 1 cut(s) 1062
RsaI GTAC 4 cut(s) 287, 304, 443, 667
RsaNI GTAC 4 cut(s) 286, 303, 442, 666
SalI GTCGAC 1 cut(s) 1202
SapI GCTCTTC 1 cut(s) 1041
SaqAI TTAA 7 cut(s) 381, 716, 729, 810, 917, 1128, 1487
SatI GCNGC 6 cut(s) 255, 322, 468, 521, 801, 1525
Sau3AI GATC 3 cut(s) 910, 1059, 1337
Sau96I GGNCC 7 cut(s) 176, 1156, 1157, 1251, 1282, 1342, 1369
ScaI AGTACT 1 cut(s) 667
SchI GAGTC 1 cut(s) 270
ScrFI CCNGG 8 cut(s) 129, 173, 329, 1142, 1155, 1268, 1385, 1386
SduI GDGCHC 6 cut(s) 400, 1140, 1149, 1160, 1177, 1416
SfaNI GCATC 2 cut(s) 256, 913
SfcI CTRYAG 4 cut(s) 417, 468, 1407, 1467
SfoI GGCGCC 1 cut(s) 449
Sfr274I CTCGAG 1 cut(s) 393
SfuI TTCGAA 1 cut(s) 1100
SinI GGWCC 3 cut(s) 176, 1282, 1342
SlaI CTCGAG 1 cut(s) 393
SmaI CCCGGG 1 cut(s) 1386
SmlI CTYRAG 1 cut(s) 393
SmoI CTYRAG 1 cut(s) 393
Sse9I AATT 8 cut(s) 495, 624, 717, 730, 794, 828, 914, 1442
SsiI CCGC 7 cut(s) 154, 390, 800, 1306, 1460, 1525, 1535
SspDI GGCGCC 1 cut(s) 447
SspI AATATT 1 cut(s) 597
SspMI CTAG 5 cut(s) 293, 350, 723, 893, 1397
StyD4I CCNGG 8 cut(s) 127, 171, 327, 1140, 1153, 1266, 1383, 1384
StyI CCWWGG 5 cut(s) 349, 769, 1035, 1236, 1286
TaaI ACNGT 4 cut(s) 290, 374, 976, 1411
TaiI ACGT 6 cut(s) 310, 528, 680, 1083, 1197, 1203
TaqI TCGA 6 cut(s) 394, 408, 961, 1100, 1203, 1328
TasI AATT 8 cut(s) 495, 624, 717, 730, 794, 828, 914, 1442
TatI WGTACW 3 cut(s) 285, 302, 665
TauI GCSGC 2 cut(s) 803, 1527
TfiI GAWTC 2 cut(s) 1069, 1088
Tru1I TTAA 7 cut(s) 381, 716, 729, 810, 917, 1128, 1487
Tru9I TTAA 7 cut(s) 381, 716, 729, 810, 917, 1128, 1487
TscAI CASTG 5 cut(s) 186, 868, 909, 979, 1416
TseFI GTSAC 2 cut(s) 788, 872
TseI GCWGC 4 cut(s) 254, 321, 467, 520
Tsp45I GTSAC 2 cut(s) 788, 872
TspDTI ATGAA 3 cut(s) 17, 1300, 1520
TspMI CCCGGG 1 cut(s) 1384
TspRI CASTG 5 cut(s) 186, 868, 909, 979, 1416
Tth111I GACNNNGTC 1 cut(s) 1208
VneI GTGCAC 2 cut(s) 1145, 1412
VpaK11BI GGWCC 3 cut(s) 176, 1282, 1342
XceI RCATGY 2 cut(s) 481, 1249
XcmI CCANNNNNNNNNTGG 1 cut(s) 1167
XhoI CTCGAG 1 cut(s) 393
XmaI CCCGGG 1 cut(s) 1384
XmaJI CCTAGG 1 cut(s) 349
XmiI GTMKAC 2 cut(s) 1203, 1470
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 5 cut(s) 293, 350, 723, 893, 1397
ZraI GACGTC 1 cut(s) 1195
ZrmI AGTACT 1 cut(s) 667
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.