FvH4_4g26050

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015960
Physical Location & Seq
Reverse (-)
27610178 .. 27613139
2962 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26050.t1

Sequence Viewer

Length: 1434 bp
ATGCACATAAATCTCAAAATGCAGATGAAGAGAAGCATAACTTCATCCACTCTACTACTATTTTGCGTGATTGTTCTCGTCACATTTTCCATAACTTCAGTTGAGGCCAGAAAGCACCAAACCAAGAAGACCAAACCTCATAAACATCAAAAAGGTGGTAATGGCACTCATGGTTCACATTTGCCTTGTCCTGCTCCAACTCCAGTTCCTCAGGATGGTTCCTTTTCTGTCTTGTCTTTTGGAGCCAAGGGTGATGGTGTCTCCGACGATTCTAAGGCGCTCACAGCAGCATGGAAAGCTGCCTGCAAAGTGAATGGGGCTAGTGTGGAAATCCCTTCAGGATTCAAGTTCCTCATCAAGCCAATAACTCTTCAAGGCCCTTGTATGCCTAACCTTGTTCTTCAGATAGATGGAACTCTAATGGCTCCTCCAAAAGTTGGTTCATGGTCAAAGTCCAGTCTTTTTCAGTGGATAAACTTCAAATGGGTTCATAACTTCACCATCCAGGGCACCGGAGTTGTCGATGGCCAGGGCTTTGACTGGTGGAGTAGTTGTAGTTCCTCTTCATCTTCAGTCTGTTACTTGCAGAAGAAGTCCAATAAGCATATCCCAAATTTCAAACCCACAGCTTTGAGATTCTATTCAAGCTATGATGTCATAGTACGAGATATTAGAATCATCAACAGCCCTCAGTGCCATTTGAAATTTGACAGCTCTGGAGGGATCAAAGTTAACAACATTACCATATCTTCACCAGAGAATAGCCCAAATACTGATGGAATTCACTTGCAGAACACCCAAAACGTCGAAATCCATCATTCTACTATTGGTTGTGGAGATGACTGTGTATCCATACAAACTGGCTGCTCCAATGTTCATGTTCATCATATCAATTGTGGCCCTGGCCATGGCATAAGTCTAGGAGGACTTGGAAAAGATAGAAGTGTTGCTTGTGTCTCAAACATAATTGTCAATGACGTTTCATTTCAGAACACTCTATCAGGAGTCAGGATCAAAACTTGGCAGGGAGGCATTGGGTCTGTGAAGAATGTATCGTTTTCCAATATCCAAGTTTCGGACGTGAAGGTTCCTGTTATGATAGACCAGTACTACTGTGACAAGAAGTCCTGCAAGAACCAAACAGGAGCAGTAGCAATTTCGGGTGTGAAATATGACCAAATAATTGGGACTTATTCAGTGCAGCCTGTTCATCTCGCTTGCAGCAGCGACGTGCCATGCACAGATGTGGACCTTATTGATATTCAGCTCAAGCCCTCAGCAGGGTACCAGAGATTTCAGCACGCTTTGTGTTGGAACTCTTATGGCAAGTCACAACCTCCATTAGTGCCTTCAAGCATAGACTACTGCTTGAGAAGAGATGAAAATCCAGTCAAGAGAATAGCAAGGTCACATGATCACCAGCATCTGTGCTAG

Protein Analysis

478

Amino Acids

51.96

Weight (kDa)

9.03

Isoelectric Point (pI)

46.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 107 - 426 1.7e-77 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26610
fragaria_vesca FvH4_4g26050
malus_domestica MD16G1097700.v1.1
prunus_persica Prupe.1G249200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0433511
rosa_laevigata RLG00000006749
rosa_multiflora Rmu_sc0000312.1_g000004
rosa_roxburghii Rroxscaffold_5G00375030
rosa_rugosa Rorug04G0269200
rosa_samantha Rh4AG323800 Rh4BG331800 Rh4CG347100 Rh4DG328300
rosa_wichuraiana Rw0G020120 Rw4G028060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1284
AccB1I GGYRCC 2 cut(s) 509, 1284
AccB7I CCANNNNNTGG 1 cut(s) 437
AclWI GGATC 2 cut(s) 731, 1019
AcoI YGGCCR 2 cut(s) 526, 904
AcsI RAATTY 3 cut(s) 613, 704, 780
AcuI CTGAAG 4 cut(s) 81, 321, 386, 555
AfaI GTAC 3 cut(s) 663, 1109, 1286
AfiI CCNNNNNNNGG 6 cut(s) 215, 437, 908, 1075, 1280, 1281
AgsI TTSAA 7 cut(s) 346, 374, 481, 619, 645, 703, 1353
AhdI GACNNNNNGTC 1 cut(s) 1123
AjiI CACGTC 2 cut(s) 1081, 1231
AjnI CCWGG 3 cut(s) 504, 528, 901
AjuI GAANNNNNNNTTGG 2 cut(s) 424, 456
AleI CACNNNNGTG 1 cut(s) 1244
AluBI AGCT 5 cut(s) 299, 629, 648, 714, 1267
AluI AGCT 5 cut(s) 299, 629, 648, 714, 1267
Alw26I GTCTC 2 cut(s) 265, 961
AlwI GGATC 2 cut(s) 731, 1019
AlwNI CAGNNNCTG 1 cut(s) 1426
AoxI GGCC 5 cut(s) 105, 376, 526, 898, 904
ApeKI GCWGC 6 cut(s) 287, 299, 864, 1201, 1221, 1224
ApoI RAATTY 3 cut(s) 613, 704, 780
Asp718I GGTACC 1 cut(s) 1284
AspLEI GCGC 1 cut(s) 280
AspS9I GGNCC 3 cut(s) 377, 899, 1249
AsuHPI GGTGA 4 cut(s) 263, 490, 744, 1409
AvaII GGWCC 1 cut(s) 1249
AxyI CCTNAGG 1 cut(s) 210
BaeGI GKGCMC 1 cut(s) 512
BalI TGGCCA 2 cut(s) 528, 906
BanI GGYRCC 2 cut(s) 509, 1284
BarI GAAGNNNNNNTAC 2 cut(s) 547, 579
BbsI GAAGAC 1 cut(s) 134
BbvCI CCTCAGC 1 cut(s) 1276
BbvI GCAGC 6 cut(s) 286, 299, 851, 1213, 1233, 1236
BccI CCATC 7 cut(s) 209, 248, 404, 509, 518, 770, 822
BciT130I CCWGG 3 cut(s) 506, 530, 903
BciVI GTATCC 1 cut(s) 859
BclI TGATCA 1 cut(s) 1414
BcoDI GTCTC 2 cut(s) 265, 961
BfaI CTAG 3 cut(s) 321, 920, 1432
BfoI RGCGCY 1 cut(s) 281
BfuI GTATCC 1 cut(s) 859
BisI GCNGC 6 cut(s) 288, 300, 865, 1202, 1222, 1225
BlsI GCNGC 6 cut(s) 289, 301, 866, 1203, 1223, 1226
BmcAI AGTACT 1 cut(s) 1109
Bme1390I CCNGG 3 cut(s) 506, 530, 903
Bme18I GGWCC 1 cut(s) 1249
BmeRI GACNNNNNGTC 1 cut(s) 1123
BmgBI CACGTC 2 cut(s) 1081, 1231
BmgT120I GGNCC 3 cut(s) 377, 899, 1249
BmiI GGNNCC 6 cut(s) 220, 244, 426, 511, 1089, 1286
BmrFI CCNGG 3 cut(s) 506, 530, 903
BpiI GAAGAC 1 cut(s) 134
BpmI CTGGAG 2 cut(s) 186, 738
Bpu10I CCTNAGC 1 cut(s) 1276
BpuEI CTTGAG 2 cut(s) 1253, 1390
BsaBI GATNNNNATC 1 cut(s) 1383
BsaJI CCNNGG 5 cut(s) 246, 505, 529, 901, 907
BsaWI WCCGGW 1 cut(s) 512
BsaXI ACNNNNNCTCC 2 cut(s) 538, 568
Bsc4I CCNNNNNNNGG 6 cut(s) 215, 437, 908, 1075, 1280, 1281
Bse1I ACTGG 6 cut(s) 203, 456, 545, 865, 1105, 1388
Bse21I CCTNAGG 1 cut(s) 210
Bse8I GATNNNNATC 1 cut(s) 1383
BseBI CCWGG 3 cut(s) 506, 530, 903
BseDI CCNNGG 5 cut(s) 246, 505, 529, 901, 907
BseGI GGATG 3 cut(s) 44, 220, 501
BseJI GATNNNNATC 1 cut(s) 1383
BseLI CCNNNNNNNGG 6 cut(s) 215, 437, 908, 1075, 1280, 1281
BseMII CTCAG 3 cut(s) 224, 704, 1290
BseNI ACTGG 6 cut(s) 203, 456, 545, 865, 1105, 1388
BseRI GAGGAG 1 cut(s) 417
BseSI GKGCMC 1 cut(s) 512
BseXI GCAGC 6 cut(s) 286, 299, 851, 1213, 1233, 1236
BsgI GTGCAG 1 cut(s) 1220
BshFI GGCC 5 cut(s) 107, 378, 528, 900, 906
BshNI GGYRCC 2 cut(s) 509, 1284
BsiSI CCGG 1 cut(s) 513
BslFI GGGAC 1 cut(s) 1201
BslI CCNNNNNNNGG 6 cut(s) 215, 437, 908, 1075, 1280, 1281
BsmAI GTCTC 2 cut(s) 265, 961
BsmFI GGGAC 1 cut(s) 1201
BsnI GGCC 5 cut(s) 107, 378, 528, 900, 906
Bsp1286I GDGCHC 1 cut(s) 512
Bsp143I GATC 3 cut(s) 723, 1011, 1414
Bsp19I CCATGG 1 cut(s) 907
BspANI GGCC 5 cut(s) 107, 378, 528, 900, 906
BspCNI CTCAG 3 cut(s) 223, 703, 1289
BspLI GGNNCC 6 cut(s) 220, 244, 426, 511, 1089, 1286
BspPI GGATC 2 cut(s) 731, 1019
BspT107I GGYRCC 2 cut(s) 509, 1284
BsrI ACTGG 6 cut(s) 203, 456, 545, 865, 1105, 1388
BssECI CCNNGG 5 cut(s) 246, 505, 529, 901, 907
BssMI GATC 3 cut(s) 723, 1011, 1414
BssT1I CCWWGG 2 cut(s) 246, 907
Bst2UI CCWGG 3 cut(s) 506, 530, 903
Bst4CI ACNGT 2 cut(s) 845, 1115
Bst6I CTCTTC 4 cut(s) 23, 375, 568, 1369
BstC8I GCNNGC 3 cut(s) 304, 1219, 1302
BstDEI CTNAG 4 cut(s) 210, 273, 690, 1276
BstDSI CCRYGG 1 cut(s) 907
BstF5I GGATG 3 cut(s) 44, 220, 501
BstH2I RGCGCY 1 cut(s) 281
BstHHI GCGC 1 cut(s) 280
BstKTI GATC 3 cut(s) 726, 1014, 1417
BstMAI GTCTC 2 cut(s) 265, 961
BstMBI GATC 3 cut(s) 723, 1011, 1414
BstMWI GCNNNNNNNGC 3 cut(s) 284, 296, 693
BstNI CCWGG 3 cut(s) 506, 530, 903
BstSCI CCNGG 3 cut(s) 504, 528, 901
BstSLI GKGCMC 1 cut(s) 512
BstV1I GCAGC 6 cut(s) 286, 299, 851, 1213, 1233, 1236
BstV2I GAAGAC 1 cut(s) 134
BstXI CCANNNNNNTGG 1 cut(s) 1184
Bsu36I CCTNAGG 1 cut(s) 210
BsuI GTATCC 1 cut(s) 859
BsuRI GGCC 5 cut(s) 107, 378, 528, 900, 906
BtgI CCRYGG 1 cut(s) 907
BtrI CACGTC 2 cut(s) 1081, 1231
BtsCI GGATG 3 cut(s) 44, 220, 501
BtsIMutI CAGTG 3 cut(s) 473, 698, 1203
Cac8I GCNNGC 3 cut(s) 304, 1219, 1302
CaiI CAGNNNCTG 1 cut(s) 1426
CfoI GCGC 1 cut(s) 280
Cfr13I GGNCC 3 cut(s) 377, 899, 1249
Csp6I GTAC 3 cut(s) 662, 1108, 1285
CviAII CATG 7 cut(s) 170, 291, 444, 878, 908, 1236, 1412
CviQI GTAC 3 cut(s) 662, 1108, 1285
DdeI CTNAG 4 cut(s) 210, 273, 690, 1276
DpnI GATC 3 cut(s) 725, 1013, 1416
DpnII GATC 3 cut(s) 723, 1011, 1414
DriI GACNNNNNGTC 1 cut(s) 1123
EaeI YGGCCR 2 cut(s) 526, 904
Eam1104I CTCTTC 4 cut(s) 23, 375, 568, 1369
Eam1105I GACNNNNNGTC 1 cut(s) 1123
EarI CTCTTC 4 cut(s) 23, 375, 568, 1369
Eco130I CCWWGG 2 cut(s) 246, 907
Eco47I GGWCC 1 cut(s) 1249
Eco57I CTGAAG 4 cut(s) 81, 321, 386, 555
Eco81I CCTNAGG 1 cut(s) 210
EcoO109I RGGNCCY 1 cut(s) 377
EcoRI GAATTC 1 cut(s) 780
EcoRII CCWGG 3 cut(s) 504, 528, 901
EcoT14I CCWWGG 2 cut(s) 246, 907
ErhI CCWWGG 2 cut(s) 246, 907
FaeI CATG 7 cut(s) 173, 294, 447, 881, 911, 1239, 1415
FalI AAGNNNNNCTT 4 cut(s) 25, 57, 934, 966
FaqI GGGAC 1 cut(s) 1201
FatI CATG 7 cut(s) 169, 290, 443, 877, 907, 1235, 1411
FbaI TGATCA 1 cut(s) 1414
Fnu4HI GCNGC 6 cut(s) 288, 300, 865, 1202, 1222, 1225
FokI GGATG 3 cut(s) 31, 227, 488
Fsp4HI GCNGC 6 cut(s) 288, 300, 865, 1202, 1222, 1225
FspBI CTAG 3 cut(s) 321, 920, 1432
GlaI GCGC 1 cut(s) 279
GluI GCNGC 6 cut(s) 288, 300, 865, 1202, 1222, 1225
GsuI CTGGAG 2 cut(s) 186, 738
HaeII RGCGCY 1 cut(s) 281
HaeIII GGCC 5 cut(s) 107, 378, 528, 900, 906
HapII CCGG 1 cut(s) 513
HhaI GCGC 1 cut(s) 280
Hin1II CATG 7 cut(s) 173, 294, 447, 881, 911, 1239, 1415
Hin6I GCGC 1 cut(s) 278
HinP1I GCGC 1 cut(s) 278
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HinfI GANTC 5 cut(s) 269, 342, 636, 675, 1005
HpaI GTTAAC 1 cut(s) 733
HpaII CCGG 1 cut(s) 513
HphI GGTGA 4 cut(s) 263, 490, 744, 1409
Hpy166II GTNNAC 3 cut(s) 176, 733, 1249
Hpy188I TCNGA 4 cut(s) 265, 405, 990, 1078
Hpy188III TCNNGA 6 cut(s) 212, 339, 717, 1002, 1009, 1393
Hpy8I GTNNAC 3 cut(s) 176, 733, 1249
Hpy99I CGWCG 3 cut(s) 269, 809, 1232
HpyAV CCTTC 3 cut(s) 345, 1078, 1359
HpyCH4III ACNGT 2 cut(s) 845, 1115
HpyCH4IV ACGT 4 cut(s) 804, 978, 1080, 1230
HpyCH4V TGCA 9 cut(s) 4, 22, 306, 586, 790, 1131, 1201, 1221, 1239
HpyF10VI GCNNNNNNNGC 3 cut(s) 284, 296, 693
HpyF3I CTNAG 4 cut(s) 210, 273, 690, 1276
HpySE526I ACGT 4 cut(s) 804, 978, 1080, 1230
Hsp92II CATG 7 cut(s) 173, 294, 447, 881, 911, 1239, 1415
HspAI GCGC 1 cut(s) 278
KpnI GGTACC 1 cut(s) 1288
Ksp22I TGATCA 1 cut(s) 1414
KspAI GTTAAC 1 cut(s) 733
Kzo9I GATC 3 cut(s) 723, 1011, 1414
LmnI GCTCC 5 cut(s) 199, 242, 430, 872, 1145
Lsp1109I GCAGC 6 cut(s) 286, 299, 851, 1213, 1233, 1236
MaeI CTAG 3 cut(s) 321, 920, 1432
MaeII ACGT 4 cut(s) 804, 978, 1080, 1230
MaeIII GTNAC 5 cut(s) 79, 578, 1115, 1329, 1407
MalI GATC 3 cut(s) 725, 1013, 1416
MboI GATC 3 cut(s) 723, 1011, 1414
MfeI CAATTG 1 cut(s) 892
MhlI GDGCHC 1 cut(s) 512
MlsI TGGCCA 2 cut(s) 528, 906
MluCI AATT 7 cut(s) 613, 704, 780, 892, 966, 1155, 1182
MluNI TGGCCA 2 cut(s) 528, 906
MlyI GAGTC 1 cut(s) 1014
MmeI TCCRAC 3 cut(s) 221, 288, 1292
Mox20I TGGCCA 2 cut(s) 528, 906
MscI TGGCCA 2 cut(s) 528, 906
MseI TTAA 1 cut(s) 732
MslI CAYNNNNRTG 1 cut(s) 1244
Msp20I TGGCCA 2 cut(s) 528, 906
MspI CCGG 1 cut(s) 513
MspR9I CCNGG 3 cut(s) 506, 530, 903
MunI CAATTG 1 cut(s) 892
MvaI CCWGG 3 cut(s) 506, 530, 903
MwoI GCNNNNNNNGC 3 cut(s) 284, 296, 693
NcoI CCATGG 1 cut(s) 907
NdeII GATC 3 cut(s) 723, 1011, 1414
NlaIII CATG 7 cut(s) 173, 294, 447, 881, 911, 1239, 1415
NlaIV GGNNCC 6 cut(s) 220, 244, 426, 511, 1089, 1286
NmuCI GTSAC 4 cut(s) 79, 1115, 1329, 1407
OliI CACNNNNGTG 1 cut(s) 1244
PfeI GAWTC 4 cut(s) 269, 342, 636, 675
PflMI CCANNNNNTGG 1 cut(s) 437
PkrI GCNGC 6 cut(s) 289, 301, 866, 1203, 1223, 1226
PleI GAGTC 1 cut(s) 1013
PpsI GAGTC 1 cut(s) 1013
Psp6I CCWGG 3 cut(s) 504, 528, 901
PspGI CCWGG 3 cut(s) 504, 528, 901
PspN4I GGNNCC 6 cut(s) 220, 244, 426, 511, 1089, 1286
PspPI GGNCC 3 cut(s) 377, 899, 1249
PsrI GAACNNNNNNTAC 2 cut(s) 541, 573
PstNI CAGNNNCTG 1 cut(s) 1426
RsaI GTAC 3 cut(s) 663, 1109, 1286
RsaNI GTAC 3 cut(s) 662, 1108, 1285
RseI CAYNNNNRTG 1 cut(s) 1244
SaqAI TTAA 1 cut(s) 732
SatI GCNGC 6 cut(s) 288, 300, 865, 1202, 1222, 1225
Sau3AI GATC 3 cut(s) 723, 1011, 1414
Sau96I GGNCC 3 cut(s) 377, 899, 1249
ScaI AGTACT 1 cut(s) 1109
SchI GAGTC 1 cut(s) 1014
ScrFI CCNGG 3 cut(s) 506, 530, 903
SduI GDGCHC 1 cut(s) 512
SinI GGWCC 1 cut(s) 1249
SmiMI CAYNNNNRTG 1 cut(s) 1244
SmlI CTYRAG 2 cut(s) 1268, 1369
SmoI CTYRAG 2 cut(s) 1268, 1369
Sse9I AATT 7 cut(s) 613, 704, 780, 892, 966, 1155, 1182
SspMI CTAG 3 cut(s) 321, 920, 1432
StyD4I CCNGG 3 cut(s) 504, 528, 901
StyI CCWWGG 2 cut(s) 246, 907
TaaI ACNGT 2 cut(s) 845, 1115
TaiI ACGT 4 cut(s) 807, 981, 1083, 1233
TaqI TCGA 2 cut(s) 522, 807
TasI AATT 7 cut(s) 613, 704, 780, 892, 966, 1155, 1182
TatI WGTACW 1 cut(s) 1107
TfiI GAWTC 4 cut(s) 269, 342, 636, 675
Tru1I TTAA 1 cut(s) 732
Tru9I TTAA 1 cut(s) 732
TscAI CASTG 3 cut(s) 473, 698, 1203
TseFI GTSAC 4 cut(s) 79, 1115, 1329, 1407
TseI GCWGC 6 cut(s) 287, 299, 864, 1201, 1221, 1224
Tsp45I GTSAC 4 cut(s) 79, 1115, 1329, 1407
TspRI CASTG 3 cut(s) 473, 698, 1203
Van91I CCANNNNNTGG 1 cut(s) 437
VpaK11BI GGWCC 1 cut(s) 1249
XapI RAATTY 3 cut(s) 613, 704, 780
XspI CTAG 3 cut(s) 321, 920, 1432
ZrmI AGTACT 1 cut(s) 1109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.