Rroxscaffold_5G00375030

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015960
Physical Location & Seq
Reverse (-)
55913179 .. 55917698
4520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00375030.1

Sequence Viewer

Length: 1491 bp
ATGCGTGCGCCGGGACGGCCGGCCGATGGTATTGTTGCTTCCGGTGGAGTTAGTTACGGCGATGGCAACAAGGTTTTCGATGGTGGTCGGGAGATGACTTCATCCACTCTACTACTGTTTTTCTGGATTGTTCTTGTCACATTTTCCATAACTTCGGTTGAGGCAAGAAAGCACCAAACCAAGAAGAGCAAACCCCAAAAACATCAAAAAGGTGGTAATAACACTCATGATTCACATTTGCCTCGTCCTGCTCCAACTCCAGTTCCTCAGTATGGTTCCTTTTCTGTCTTGTCCTTTGGAGCCAAGGGTGATGGTGTCTCCGACGATTCTAAGGCGCTCATAGCGGCATGGAAAGCTGCCTGCAAAGTGAATGGAGCTAGTGTAGAAATCCCTTCAGGATTCAAGTTCCTCATCAAGCCAATAACTCTTCAAGGCCCTTGTATGCCTGACGTTGTTCTTCAGATAGATGGAACTCTAATGGGTCCTCCAAAAGTTAGCTCATGGTCAAAGTCCAGTTTGTTTCAGTGGATAAACTTCAAATGGGTTCATAACTTCACCATCCAGGGCACCGGAGTTGTCGATGGCCAGGGCTCTGACTGGTGGACTAGTAGTTCCTCTTCCTCTTCAATCTATAATTACTTGCAGAAGAAGTCCAACAAGCATATGCCGAATTTCAAACCCACTGCTTTGAGATTCTATTCAAGCTATGATGTGACAGTACGAGATATCAGAATCATAAACAGCCCTCAGTGCCATTTGAAATTTGACAGCTCTGGAGGGATCATAGTTGACAACATTACCATATCTTCACCAGAGAATGGTCCAAATACTGATGGAATTCACTTGCAGAACACCCAAAACGTCGAAATTCATCATTCTACTATTGGTTGTGGAGATGACTGTGTATCCATACAAACTGGCTGCTCCAATGTTCATGTTCATCATATCAATTGTGGCCCTGGCCATGGCATAAGTCTAGGAGGACTTGGAAAAGACAGAAGTGTTGCTTGTGTCTCAAATATAATTGTCAATGACATTACATTTCAAAACACTTTATCTGGAGTTAGGATCAAAACATGGCAGGGAGGCATTGGGTCTGTGAAGAATGTATCTTTTTCCAATATCCAAGTTTCGAATGTGAAGGTTCCGATAATGATAGACCAGTACTACTGTGACAAGAAGTCCTGCAAGAACCAAACAGGAGCTGTAGCAATCTCAGGTGTGAAATATGACCAAATAATTGGGACTTATTCGGTGCAGCCTGTTCATCTTGCTTGCAGCAGCGACATTCCATGCACAGATGTTGACCTCATTGATATTCAGCTCAAGCCCTCAGCAGGGTACCAGAGATTTCAACACGCTTTGTGTTGGAACTCTTATGGCAAGTCACAAGCTCCTTTGGTCCCTTCAAGCATAGACTACTGCTTGAGAAGAGATGAAAATCCAGTCGAGAGAATAGCAAAGTCACATGATCGTCAGCATTTGTGCTAG

Protein Analysis

496

Amino Acids

53.91

Weight (kDa)

8.84

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 126 - 445 3.7e-79 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26610
fragaria_vesca FvH4_4g26050
malus_domestica MD16G1097700.v1.1
prunus_persica Prupe.1G249200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0433511
rosa_laevigata RLG00000006749
rosa_multiflora Rmu_sc0000312.1_g000004
rosa_roxburghii Rroxscaffold_5G00375030
rosa_rugosa Rorug04G0269200
rosa_samantha Rh4AG323800 Rh4BG331800 Rh4CG347100 Rh4DG328300
rosa_wichuraiana Rw0G020120 Rw4G028060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1341
AccB1I GGYRCC 2 cut(s) 566, 1341
AccB7I CCANNNNNTGG 1 cut(s) 818
AciI CCGC 1 cut(s) 344
AclWI GGATC 2 cut(s) 788, 1076
AcoI YGGCCR 4 cut(s) 17, 21, 583, 961
AcsI RAATTY 4 cut(s) 670, 761, 837, 867
AcuI CTGAAG 2 cut(s) 378, 443
AfaI GTAC 3 cut(s) 720, 1166, 1343
AfiI CCNNNNNNNGG 6 cut(s) 26, 272, 818, 965, 1337, 1338
AhdI GACNNNNNGTC 1 cut(s) 1180
AhlI ACTAGT 1 cut(s) 605
AjnI CCWGG 3 cut(s) 561, 585, 958
AloI GAACNNNNNNTCC 2 cut(s) 595, 627
AluBI AGCT 8 cut(s) 356, 377, 498, 705, 771, 1205, 1324, 1394
AluI AGCT 8 cut(s) 356, 377, 498, 705, 771, 1205, 1324, 1394
Alw26I GTCTC 2 cut(s) 322, 1018
AlwI GGATC 2 cut(s) 788, 1076
AlwNI CAGNNNCTG 1 cut(s) 1205
AoxI GGCC 6 cut(s) 17, 21, 433, 583, 955, 961
ApeKI GCWGC 5 cut(s) 356, 921, 1258, 1278, 1281
ApoI RAATTY 4 cut(s) 670, 761, 837, 867
Asp718I GGTACC 1 cut(s) 1341
AspLEI GCGC 2 cut(s) 10, 337
AspS9I GGNCC 5 cut(s) 434, 482, 821, 956, 1402
AsuC2I CCSGG 1 cut(s) 12
AsuHPI GGTGA 3 cut(s) 320, 547, 801
AsuII TTCGAA 1 cut(s) 1133
AvaII GGWCC 3 cut(s) 482, 821, 1402
BaeGI GKGCMC 1 cut(s) 569
BalI TGGCCA 2 cut(s) 585, 963
BanI GGYRCC 2 cut(s) 566, 1341
BanII GRGCYC 1 cut(s) 593
BarI GAAGNNNNNNTAC 4 cut(s) 22, 54, 601, 633
BbvCI CCTCAGC 1 cut(s) 1333
BbvI GCAGC 5 cut(s) 343, 908, 1270, 1290, 1293
BccI CCATC 8 cut(s) 20, 56, 74, 305, 461, 566, 575, 827
BceAI ACGGC 2 cut(s) 32, 73
BciT130I CCWGG 3 cut(s) 563, 587, 960
BciVI GTATCC 1 cut(s) 916
BcnI CCSGG 1 cut(s) 12
BcoDI GTCTC 2 cut(s) 322, 1018
BcuI ACTAGT 1 cut(s) 605
BfaI CTAG 4 cut(s) 378, 606, 977, 1489
BfmI CTRYAG 1 cut(s) 1206
BfoI RGCGCY 1 cut(s) 338
BfuI GTATCC 1 cut(s) 916
BglI GCCNNNNNGGC 1 cut(s) 16
BisI GCNGC 6 cut(s) 345, 357, 922, 1259, 1279, 1282
BlsI GCNGC 6 cut(s) 346, 358, 923, 1260, 1280, 1283
BmcAI AGTACT 1 cut(s) 1166
Bme1390I CCNGG 4 cut(s) 12, 563, 587, 960
Bme18I GGWCC 3 cut(s) 482, 821, 1402
BmeRI GACNNNNNGTC 1 cut(s) 1180
BmgT120I GGNCC 5 cut(s) 434, 482, 821, 956, 1402
BmiI GGNNCC 7 cut(s) 277, 301, 483, 568, 1146, 1343, 1404
BmrFI CCNGG 4 cut(s) 12, 563, 587, 960
BpmI CTGGAG 3 cut(s) 243, 795, 1080
Bpu10I CCTNAGC 1 cut(s) 1333
Bpu14I TTCGAA 1 cut(s) 1133
BpuEI CTTGAG 2 cut(s) 1310, 1447
BpuMI CCSGG 1 cut(s) 12
BsaBI GATNNNNATC 1 cut(s) 1440
BsaJI CCNNGG 5 cut(s) 303, 562, 586, 958, 964
BsaWI WCCGGW 2 cut(s) 41, 569
BsaXI ACNNNNNCTCC 2 cut(s) 366, 396
Bsc4I CCNNNNNNNGG 6 cut(s) 26, 272, 818, 965, 1337, 1338
Bse118I RCCGGY 1 cut(s) 19
Bse1I ACTGG 6 cut(s) 260, 513, 602, 922, 1162, 1445
Bse8I GATNNNNATC 1 cut(s) 1440
BseBI CCWGG 3 cut(s) 563, 587, 960
BseDI CCNNGG 5 cut(s) 303, 562, 586, 958, 964
BseGI GGATG 2 cut(s) 101, 558
BseJI GATNNNNATC 1 cut(s) 1440
BseLI CCNNNNNNNGG 6 cut(s) 26, 272, 818, 965, 1337, 1338
BseMII CTCAG 4 cut(s) 281, 761, 1230, 1347
BseNI ACTGG 6 cut(s) 260, 513, 602, 922, 1162, 1445
BseSI GKGCMC 1 cut(s) 569
BseX3I CGGCCG 2 cut(s) 17, 21
BseXI GCAGC 5 cut(s) 343, 908, 1270, 1290, 1293
BsgI GTGCAG 1 cut(s) 1277
Bsh1285I CGRYCG 2 cut(s) 20, 24
BshFI GGCC 6 cut(s) 19, 23, 435, 585, 957, 963
BshNI GGYRCC 2 cut(s) 566, 1341
BsiEI CGRYCG 2 cut(s) 20, 24
BsiSI CCGG 4 cut(s) 11, 20, 42, 570
BslFI GGGAC 3 cut(s) 27, 1258, 1388
BslI CCNNNNNNNGG 6 cut(s) 26, 272, 818, 965, 1337, 1338
BsmAI GTCTC 2 cut(s) 322, 1018
BsmFI GGGAC 3 cut(s) 27, 1258, 1388
BsnI GGCC 6 cut(s) 19, 23, 435, 585, 957, 963
Bsp119I TTCGAA 1 cut(s) 1133
Bsp1286I GDGCHC 2 cut(s) 569, 593
Bsp143I GATC 3 cut(s) 780, 1068, 1471
Bsp19I CCATGG 1 cut(s) 964
BspACI CCGC 1 cut(s) 344
BspANI GGCC 6 cut(s) 19, 23, 435, 585, 957, 963
BspCNI CTCAG 4 cut(s) 280, 760, 1229, 1346
BspHI TCATGA 1 cut(s) 226
BspLI GGNNCC 7 cut(s) 277, 301, 483, 568, 1146, 1343, 1404
BspPI GGATC 2 cut(s) 788, 1076
BspQI GCTCTTC 1 cut(s) 179
BspT104I TTCGAA 1 cut(s) 1133
BspT107I GGYRCC 2 cut(s) 566, 1341
BsrFI RCCGGY 1 cut(s) 19
BsrI ACTGG 6 cut(s) 260, 513, 602, 922, 1162, 1445
BssAI RCCGGY 1 cut(s) 19
BssECI CCNNGG 5 cut(s) 303, 562, 586, 958, 964
BssMI GATC 3 cut(s) 780, 1068, 1471
BssT1I CCWWGG 2 cut(s) 303, 964
Bst2UI CCWGG 3 cut(s) 563, 587, 960
Bst4CI ACNGT 4 cut(s) 117, 718, 902, 1172
Bst6I CTCTTC 5 cut(s) 179, 432, 622, 628, 1426
BstBI TTCGAA 1 cut(s) 1133
BstC8I GCNNGC 4 cut(s) 6, 21, 361, 1276
BstDEI CTNAG 5 cut(s) 267, 330, 747, 1216, 1333
BstDSI CCRYGG 1 cut(s) 964
BstF5I GGATG 2 cut(s) 101, 558
BstH2I RGCGCY 1 cut(s) 338
BstHHI GCGC 2 cut(s) 10, 337
BstKTI GATC 3 cut(s) 783, 1071, 1474
BstMAI GTCTC 2 cut(s) 322, 1018
BstMBI GATC 3 cut(s) 780, 1068, 1471
BstMCI CGRYCG 2 cut(s) 20, 24
BstMWI GCNNNNNNNGC 4 cut(s) 16, 341, 353, 750
BstNI CCWGG 3 cut(s) 563, 587, 960
BstSCI CCNGG 4 cut(s) 10, 561, 585, 958
BstSFI CTRYAG 1 cut(s) 1206
BstSLI GKGCMC 1 cut(s) 569
BstV1I GCAGC 5 cut(s) 343, 908, 1270, 1290, 1293
BstXI CCANNNNNNTGG 1 cut(s) 1241
BstZI CGGCCG 2 cut(s) 17, 21
BsuI GTATCC 1 cut(s) 916
BsuRI GGCC 6 cut(s) 19, 23, 435, 585, 957, 963
BtgI CCRYGG 1 cut(s) 964
BtgZI GCGATG 1 cut(s) 75
BtsCI GGATG 2 cut(s) 101, 558
BtsI GCAGTG 1 cut(s) 681
BtsIMutI CAGTG 3 cut(s) 530, 681, 755
Cac8I GCNNGC 4 cut(s) 6, 21, 361, 1276
CaiI CAGNNNCTG 1 cut(s) 1205
CciI TCATGA 1 cut(s) 226
CfoI GCGC 2 cut(s) 10, 337
Cfr10I RCCGGY 1 cut(s) 19
Cfr13I GGNCC 5 cut(s) 434, 482, 821, 956, 1402
Csp6I GTAC 3 cut(s) 719, 1165, 1342
CviAII CATG 8 cut(s) 227, 348, 501, 935, 965, 1077, 1293, 1469
CviQI GTAC 3 cut(s) 719, 1165, 1342
DdeI CTNAG 5 cut(s) 267, 330, 747, 1216, 1333
DpnI GATC 3 cut(s) 782, 1070, 1473
DpnII GATC 3 cut(s) 780, 1068, 1471
DriI GACNNNNNGTC 1 cut(s) 1180
EaeI YGGCCR 4 cut(s) 17, 21, 583, 961
EagI CGGCCG 2 cut(s) 17, 21
Eam1104I CTCTTC 5 cut(s) 179, 432, 622, 628, 1426
Eam1105I GACNNNNNGTC 1 cut(s) 1180
EarI CTCTTC 5 cut(s) 179, 432, 622, 628, 1426
EclXI CGGCCG 2 cut(s) 17, 21
Eco130I CCWWGG 2 cut(s) 303, 964
Eco24I GRGCYC 1 cut(s) 593
Eco32I GATATC 1 cut(s) 727
Eco47I GGWCC 3 cut(s) 482, 821, 1402
Eco52I CGGCCG 2 cut(s) 17, 21
Eco57I CTGAAG 2 cut(s) 378, 443
EcoO109I RGGNCCY 2 cut(s) 434, 482
EcoRI GAATTC 1 cut(s) 837
EcoRII CCWGG 3 cut(s) 561, 585, 958
EcoRV GATATC 1 cut(s) 727
EcoT14I CCWWGG 2 cut(s) 303, 964
EcoT38I GRGCYC 1 cut(s) 593
ErhI CCWWGG 2 cut(s) 303, 964
FaeI CATG 8 cut(s) 230, 351, 504, 938, 968, 1080, 1296, 1472
FalI AAGNNNNNCTT 2 cut(s) 991, 1023
FaqI GGGAC 3 cut(s) 27, 1258, 1388
FatI CATG 8 cut(s) 226, 347, 500, 934, 964, 1076, 1292, 1468
FauNDI CATATG 1 cut(s) 663
Fnu4HI GCNGC 6 cut(s) 345, 357, 922, 1259, 1279, 1282
FokI GGATG 2 cut(s) 88, 545
FriOI GRGCYC 1 cut(s) 593
FseI GGCCGGCC 1 cut(s) 23
Fsp4HI GCNGC 6 cut(s) 345, 357, 922, 1259, 1279, 1282
FspBI CTAG 4 cut(s) 378, 606, 977, 1489
GlaI GCGC 2 cut(s) 9, 336
GluI GCNGC 6 cut(s) 345, 357, 922, 1259, 1279, 1282
GsuI CTGGAG 3 cut(s) 243, 795, 1080
HaeII RGCGCY 1 cut(s) 338
HaeIII GGCC 6 cut(s) 19, 23, 435, 585, 957, 963
HapII CCGG 4 cut(s) 11, 20, 42, 570
HhaI GCGC 2 cut(s) 10, 337
Hin1II CATG 8 cut(s) 230, 351, 504, 938, 968, 1080, 1296, 1472
Hin6I GCGC 2 cut(s) 8, 335
HinP1I GCGC 2 cut(s) 8, 335
HincII GTYRAC 2 cut(s) 790, 1306
HindII GTYRAC 2 cut(s) 790, 1306
HinfI GANTC 5 cut(s) 230, 326, 399, 693, 732
HpaII CCGG 4 cut(s) 11, 20, 42, 570
HphI GGTGA 3 cut(s) 320, 547, 801
Hpy166II GTNNAC 3 cut(s) 603, 790, 1306
Hpy188I TCNGA 5 cut(s) 322, 462, 595, 731, 1149
Hpy188III TCNNGA 7 cut(s) 89, 124, 227, 396, 774, 1059, 1450
Hpy8I GTNNAC 3 cut(s) 603, 790, 1306
Hpy99I CGWCG 2 cut(s) 326, 866
HpyAV CCTTC 3 cut(s) 402, 1135, 1416
HpyCH4III ACNGT 4 cut(s) 117, 718, 902, 1172
HpyCH4IV ACGT 2 cut(s) 450, 861
HpyCH4V TGCA 7 cut(s) 363, 643, 847, 1188, 1258, 1278, 1296
HpyF10VI GCNNNNNNNGC 4 cut(s) 16, 341, 353, 750
HpyF3I CTNAG 5 cut(s) 267, 330, 747, 1216, 1333
HpySE526I ACGT 2 cut(s) 450, 861
Hsp92II CATG 8 cut(s) 230, 351, 504, 938, 968, 1080, 1296, 1472
HspAI GCGC 2 cut(s) 8, 335
KpnI GGTACC 1 cut(s) 1345
KroI GCCGGC 1 cut(s) 19
KroNI GCCGGC 1 cut(s) 21
Kzo9I GATC 3 cut(s) 780, 1068, 1471
LguI GCTCTTC 1 cut(s) 179
LmnI GCTCC 6 cut(s) 256, 299, 374, 929, 1202, 1399
Lsp1109I GCAGC 5 cut(s) 343, 908, 1270, 1290, 1293
MaeI CTAG 4 cut(s) 378, 606, 977, 1489
MaeII ACGT 2 cut(s) 450, 861
MaeIII GTNAC 6 cut(s) 53, 136, 712, 1172, 1386, 1464
MalI GATC 3 cut(s) 782, 1070, 1473
MboI GATC 3 cut(s) 780, 1068, 1471
MboII GAAGA 9 cut(s) 196, 419, 449, 609, 615, 658, 798, 1114, 1443
MfeI CAATTG 1 cut(s) 949
MhlI GDGCHC 2 cut(s) 569, 593
MlsI TGGCCA 2 cut(s) 585, 963
MluCI AATT 8 cut(s) 634, 670, 761, 837, 867, 949, 1023, 1239
MluNI TGGCCA 2 cut(s) 585, 963
MmeI TCCRAC 4 cut(s) 278, 345, 678, 1349
Mox20I TGGCCA 2 cut(s) 585, 963
MroNI GCCGGC 1 cut(s) 19
MscI TGGCCA 2 cut(s) 585, 963
Msp20I TGGCCA 2 cut(s) 585, 963
MspI CCGG 4 cut(s) 11, 20, 42, 570
MspR9I CCNGG 4 cut(s) 12, 563, 587, 960
MunI CAATTG 1 cut(s) 949
MvaI CCWGG 3 cut(s) 563, 587, 960
MwoI GCNNNNNNNGC 4 cut(s) 16, 341, 353, 750
NaeI GCCGGC 1 cut(s) 21
NciI CCSGG 1 cut(s) 12
NcoI CCATGG 1 cut(s) 964
NdeI CATATG 1 cut(s) 663
NdeII GATC 3 cut(s) 780, 1068, 1471
NgoMIV GCCGGC 1 cut(s) 19
NlaIII CATG 8 cut(s) 230, 351, 504, 938, 968, 1080, 1296, 1472
NlaIV GGNNCC 7 cut(s) 277, 301, 483, 568, 1146, 1343, 1404
NmuCI GTSAC 5 cut(s) 136, 712, 1172, 1386, 1464
NspV TTCGAA 1 cut(s) 1133
PagI TCATGA 1 cut(s) 226
PciSI GCTCTTC 1 cut(s) 179
PdiI GCCGGC 1 cut(s) 21
PfeI GAWTC 5 cut(s) 230, 326, 399, 693, 732
PflMI CCANNNNNTGG 1 cut(s) 818
PkrI GCNGC 6 cut(s) 346, 358, 923, 1260, 1280, 1283
PpuMI RGGWCCY 1 cut(s) 482
Psp5II RGGWCCY 1 cut(s) 482
Psp6I CCWGG 3 cut(s) 561, 585, 958
PspGI CCWGG 3 cut(s) 561, 585, 958
PspN4I GGNNCC 7 cut(s) 277, 301, 483, 568, 1146, 1343, 1404
PspPI GGNCC 5 cut(s) 434, 482, 821, 956, 1402
PspPPI RGGWCCY 1 cut(s) 482
PstNI CAGNNNCTG 1 cut(s) 1205
RigI GGCCGGCC 1 cut(s) 23
RsaI GTAC 3 cut(s) 720, 1166, 1343
RsaNI GTAC 3 cut(s) 719, 1165, 1342
SapI GCTCTTC 1 cut(s) 179
SatI GCNGC 6 cut(s) 345, 357, 922, 1259, 1279, 1282
Sau3AI GATC 3 cut(s) 780, 1068, 1471
Sau96I GGNCC 5 cut(s) 434, 482, 821, 956, 1402
ScaI AGTACT 1 cut(s) 1166
ScrFI CCNGG 4 cut(s) 12, 563, 587, 960
SduI GDGCHC 2 cut(s) 569, 593
SfcI CTRYAG 1 cut(s) 1206
SfuI TTCGAA 1 cut(s) 1133
SinI GGWCC 3 cut(s) 482, 821, 1402
SmlI CTYRAG 2 cut(s) 1325, 1426
SmoI CTYRAG 2 cut(s) 1325, 1426
SpeI ACTAGT 1 cut(s) 605
Sse9I AATT 8 cut(s) 634, 670, 761, 837, 867, 949, 1023, 1239
SsiI CCGC 1 cut(s) 344
SspMI CTAG 4 cut(s) 378, 606, 977, 1489
StyD4I CCNGG 4 cut(s) 10, 561, 585, 958
StyI CCWWGG 2 cut(s) 303, 964
TaaI ACNGT 4 cut(s) 117, 718, 902, 1172
TaiI ACGT 2 cut(s) 453, 864
TaqI TCGA 5 cut(s) 78, 579, 864, 1133, 1449
TasI AATT 8 cut(s) 634, 670, 761, 837, 867, 949, 1023, 1239
TatI WGTACW 1 cut(s) 1164
TauI GCSGC 1 cut(s) 347
TfiI GAWTC 5 cut(s) 230, 326, 399, 693, 732
TscAI CASTG 3 cut(s) 530, 688, 755
TseFI GTSAC 5 cut(s) 136, 712, 1172, 1386, 1464
TseI GCWGC 5 cut(s) 356, 921, 1258, 1278, 1281
Tsp45I GTSAC 5 cut(s) 136, 712, 1172, 1386, 1464
TspDTI ATGAA 7 cut(s) 90, 536, 860, 923, 929, 1256, 1452
TspRI CASTG 3 cut(s) 530, 688, 755
Van91I CCANNNNNTGG 1 cut(s) 818
VpaK11BI GGWCC 3 cut(s) 482, 821, 1402
XapI RAATTY 4 cut(s) 670, 761, 837, 867
XspI CTAG 4 cut(s) 378, 606, 977, 1489
ZrmI AGTACT 1 cut(s) 1166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.