FvH4_4g33090

ascorbate-specific transmembrane electron transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0017659
Physical Location & Seq
Forward (+)
31433223 .. 31434195
973 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33090.t1

Sequence Viewer

Length: 657 bp
ATGGCGCCCAAGAGTCGTACCTACCAAGTCTCAGCAACCCCAGTTGCCATAATCGCGCATTTGTTACTTATCGCTATCACAACCCTTCTCATTGTTTTTCTTGTACACTTCCGCGAGGGCTTTGCTTTCAACTCTTCCATCAAAGCGAAAATTTTCAATCTTCATCCATTTTTCATGGTGATCGGGTTTATTTTGATCGGAGGAGAAGCTATCATGGCATACAAGACAATCCCAGGAAAAAGGGATACACAAAAAGTGGTTCACATGATTTTGCATCTGTTAGCCCTGGTATCTGTATCTTTGGGGCTGTATGTAATCATCAAGTACAAAAATGAAGTGGGAGAGGTAGATTTCATTTCATTACACTCCTGGTTGGGCATAATCACCATCTCTTTATTTGGTCTTCAGTGGCTGTTAGGTTTCGTTATTTTCATCTTTCCTGGTTCAGAAATGTCAAGAAGGGCATCATTTAAACCATGGCACACTTTCTTAGGGATGGTCATTTTTCTGTTAGCAGTATGCACTGCTGAGATGGGGCTCAACTACTTCATACTAGGGAACAACCGACAAGGACTTATAGTCAACTTCACTGGCCTTTTGATTTTCTTGTTTGCCGCCGCAGTCTCCCTGACTGTACTCCTCCCCCGAATATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

219

Amino Acids

24.37

Weight (kDa)

9.83

Isoelectric Point (pI)

23.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 54 - 181 8.3e-43 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017659)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33090
malus_domestica MD13G1016700.v1.1 MD16G1014600.v1.1
prunus_persica Prupe.1G336700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0442141
rosa_laevigata RLG00000006037
rosa_roxburghii Rroxscaffold_5G00383050
rosa_rugosa Rorug04G0339200
rosa_samantha Rh4AG390800 Rh4BG404300 Rh4CG419300 Rh4DG397300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 2 cut(s) 56, 114
AciI CCGC 3 cut(s) 112, 615, 618
AcsI RAATTY 1 cut(s) 150
AcuI CTGAAG 1 cut(s) 389
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 19, 105, 326, 636
AgsI TTSAA 2 cut(s) 130, 157
AhdI GACNNNNNGTC 1 cut(s) 578
AjnI CCWGG 4 cut(s) 232, 285, 368, 439
AluBI AGCT 1 cut(s) 209
AluI AGCT 1 cut(s) 209
Alw26I GTCTC 2 cut(s) 34, 628
AlwNI CAGNNNCTG 1 cut(s) 412
AoxI GGCC 1 cut(s) 592
ApoI RAATTY 1 cut(s) 150
Asp700I GAANNNNTTC 1 cut(s) 152
AspLEI GCGC 2 cut(s) 7, 58
AsuHPI GGTGA 2 cut(s) 190, 376
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 540
BbsI GAAGAC 1 cut(s) 395
BccI CCATC 4 cut(s) 146, 395, 490, 526
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BciT130I CCWGG 4 cut(s) 234, 287, 370, 441
BciVI GTATCC 1 cut(s) 238
BcoDI GTCTC 2 cut(s) 34, 628
BfaI CTAG 1 cut(s) 554
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 238
BisI GCNGC 2 cut(s) 615, 618
BlsI GCNGC 2 cut(s) 616, 619
Bme1390I CCNGG 4 cut(s) 234, 287, 370, 441
BmeRI GACNNNNNGTC 1 cut(s) 578
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 4 cut(s) 234, 287, 370, 441
BmrI ACTGGG 1 cut(s) 35
BmsI GCATC 2 cut(s) 283, 473
BmuI ACTGGG 1 cut(s) 35
BpiI GAAGAC 1 cut(s) 395
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 232, 285, 476
Bse1I ACTGG 2 cut(s) 41, 595
BseBI CCWGG 4 cut(s) 234, 287, 370, 441
BseDI CCNNGG 3 cut(s) 232, 285, 476
BseGI GGATG 2 cut(s) 163, 501
BseMII CTCAG 2 cut(s) 45, 519
BseNI ACTGG 2 cut(s) 41, 595
BseRI GAGGAG 2 cut(s) 216, 629
Bsh1236I CGCG 2 cut(s) 56, 114
BshFI GGCC 1 cut(s) 594
BshNI GGYRCC 1 cut(s) 4
BsmAI GTCTC 2 cut(s) 34, 628
BsnI GGCC 1 cut(s) 594
Bsp1286I GDGCHC 1 cut(s) 540
Bsp1407I TGTACA 1 cut(s) 103
Bsp143I GATC 2 cut(s) 180, 195
Bsp19I CCATGG 1 cut(s) 476
BspACI CCGC 3 cut(s) 112, 615, 618
BspANI GGCC 1 cut(s) 594
BspCNI CTCAG 2 cut(s) 44, 520
BspFNI CGCG 2 cut(s) 56, 114
BspLI GGNNCC 1 cut(s) 6
BspT107I GGYRCC 1 cut(s) 4
BsrGI TGTACA 1 cut(s) 103
BsrI ACTGG 2 cut(s) 41, 595
BssECI CCNNGG 3 cut(s) 232, 285, 476
BssMI GATC 2 cut(s) 180, 195
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 476
Bst2UI CCWGG 4 cut(s) 234, 287, 370, 441
Bst4CI ACNGT 1 cut(s) 634
Bst6I CTCTTC 1 cut(s) 139
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 103
BstDEI CTNAG 3 cut(s) 31, 490, 528
BstDSI CCRYGG 1 cut(s) 476
BstF5I GGATG 2 cut(s) 163, 501
BstFNI CGCG 2 cut(s) 56, 114
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 58
BstKTI GATC 2 cut(s) 183, 198
BstMAI GTCTC 2 cut(s) 34, 628
BstMBI GATC 2 cut(s) 180, 195
BstMWI GCNNNNNNNGC 2 cut(s) 53, 215
BstNI CCWGG 4 cut(s) 234, 287, 370, 441
BstSCI CCNGG 4 cut(s) 232, 285, 368, 439
BstUI CGCG 2 cut(s) 56, 114
BstV2I GAAGAC 1 cut(s) 395
BsuI GTATCC 1 cut(s) 238
BsuRI GGCC 1 cut(s) 594
BtgI CCRYGG 1 cut(s) 476
BtsCI GGATG 2 cut(s) 163, 501
BtsI GCAGTG 1 cut(s) 522
BtsIMutI CAGTG 3 cut(s) 413, 522, 588
CaiI CAGNNNCTG 1 cut(s) 412
CfoI GCGC 2 cut(s) 7, 58
Csp6I GTAC 4 cut(s) 18, 104, 325, 635
CviAII CATG 4 cut(s) 175, 214, 265, 477
CviJI RGCY 7 cut(s) 120, 209, 284, 307, 412, 538, 594
CviKI_1 RGCY 7 cut(s) 120, 209, 284, 307, 412, 538, 594
CviQI GTAC 4 cut(s) 18, 104, 325, 635
DdeI CTNAG 3 cut(s) 31, 490, 528
DinI GGCGCC 1 cut(s) 6
DpnI GATC 2 cut(s) 182, 197
DpnII GATC 2 cut(s) 180, 195
DraI TTTAAA 1 cut(s) 472
DriI GACNNNNNGTC 1 cut(s) 578
Eam1104I CTCTTC 1 cut(s) 139
Eam1105I GACNNNNNGTC 1 cut(s) 578
EarI CTCTTC 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 476
Eco24I GRGCYC 1 cut(s) 540
Eco57I CTGAAG 1 cut(s) 389
EcoRII CCWGG 4 cut(s) 232, 285, 368, 439
EcoT14I CCWWGG 1 cut(s) 476
EcoT38I GRGCYC 1 cut(s) 540
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 476
FaeI CATG 4 cut(s) 178, 217, 268, 480
FatI CATG 4 cut(s) 174, 213, 264, 476
Fnu4HI GCNGC 2 cut(s) 615, 618
FokI GGATG 2 cut(s) 150, 508
FriOI GRGCYC 1 cut(s) 540
Fsp4HI GCNGC 2 cut(s) 615, 618
FspBI CTAG 1 cut(s) 554
GlaI GCGC 2 cut(s) 6, 57
GluI GCNGC 2 cut(s) 615, 618
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 594
HhaI GCGC 2 cut(s) 7, 58
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 178, 217, 268, 480
Hin6I GCGC 2 cut(s) 5, 56
HinP1I GCGC 2 cut(s) 5, 56
HincII GTYRAC 1 cut(s) 583
HindII GTYRAC 1 cut(s) 583
HinfI GANTC 1 cut(s) 13
HphI GGTGA 2 cut(s) 190, 376
Hpy166II GTNNAC 3 cut(s) 106, 262, 583
Hpy188I TCNGA 2 cut(s) 200, 448
Hpy188III TCNNGA 1 cut(s) 456
Hpy8I GTNNAC 3 cut(s) 106, 262, 583
HpyAV CCTTC 2 cut(s) 95, 453
HpyCH4III ACNGT 1 cut(s) 634
HpyCH4V TGCA 2 cut(s) 274, 522
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 215
HpyF3I CTNAG 3 cut(s) 31, 490, 528
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 178, 217, 268, 480
HspAI GCGC 2 cut(s) 5, 56
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 2 cut(s) 180, 195
LweI GCATC 2 cut(s) 283, 473
MaeI CTAG 1 cut(s) 554
MaeIII GTNAC 1 cut(s) 63
MalI GATC 2 cut(s) 182, 197
MboI GATC 2 cut(s) 180, 195
MboII GAAGA 3 cut(s) 126, 152, 395
MhlI GDGCHC 1 cut(s) 540
MluCI AATT 1 cut(s) 150
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 22
MnlI CCTC 4 cut(s) 109, 194, 337, 650
MroXI GAANNNNTTC 1 cut(s) 152
MseI TTAA 1 cut(s) 471
MspR9I CCNGG 4 cut(s) 234, 287, 370, 441
MvaI CCWGG 4 cut(s) 234, 287, 370, 441
MvnI CGCG 2 cut(s) 56, 114
MwoI GCNNNNNNNGC 2 cut(s) 53, 215
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 1 cut(s) 476
NdeII GATC 2 cut(s) 180, 195
NlaIII CATG 4 cut(s) 178, 217, 268, 480
NlaIV GGNNCC 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 152
PkrI GCNGC 2 cut(s) 616, 619
PleI GAGTC 1 cut(s) 21
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 21
Psp6I CCWGG 4 cut(s) 232, 285, 368, 439
PspGI CCWGG 4 cut(s) 232, 285, 368, 439
PspN4I GGNNCC 1 cut(s) 6
PstNI CAGNNNCTG 1 cut(s) 412
RsaI GTAC 4 cut(s) 19, 105, 326, 636
RsaNI GTAC 4 cut(s) 18, 104, 325, 635
SaqAI TTAA 1 cut(s) 471
SatI GCNGC 2 cut(s) 615, 618
Sau3AI GATC 2 cut(s) 180, 195
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 4 cut(s) 234, 287, 370, 441
SduI GDGCHC 1 cut(s) 540
SetI ASST 4 cut(s) 23, 211, 348, 421
SfaNI GCATC 2 cut(s) 283, 473
SfoI GGCGCC 1 cut(s) 6
Sse9I AATT 1 cut(s) 150
SsiI CCGC 3 cut(s) 112, 615, 618
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 1 cut(s) 554
StyD4I CCNGG 4 cut(s) 232, 285, 368, 439
StyI CCWWGG 1 cut(s) 476
TaaI ACNGT 1 cut(s) 634
TasI AATT 1 cut(s) 150
TatI WGTACW 3 cut(s) 103, 324, 634
TauI GCSGC 2 cut(s) 617, 620
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TscAI CASTG 3 cut(s) 413, 529, 595
TspDTI ATGAA 7 cut(s) 152, 163, 343, 348, 348, 421, 538
TspRI CASTG 3 cut(s) 413, 529, 595
XapI RAATTY 1 cut(s) 150
XmnI GAANNNNTTC 1 cut(s) 152
XspI CTAG 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.