Rroxscaffold_5G00383050

ascorbate-specific transmembrane electron transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0017659
Physical Location & Seq
Forward (+)
62755857 .. 62756881
1025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00383050.1

Sequence Viewer

Length: 657 bp
ATGGCGCCCAAGAGTCGTAGCTACCAAGTTTCAGCAACCCCAGTTACCATAATTGCGCATTTGTTACTTATAGCTATCACAACCCTTCTCCTTGTTTGGCTCGTACACTTGCGCGAAGGCTTTGCTTTCAACTCTTCCACGAAAGCAAAAATTTTCAATCTTCATCCGTTTTTTATGGTAATCGGGTTTATTTTGATCGGAGGAGAAGCTATTATGGCATACAAGACAATCCCAGGAAAAAGGGATACACAAAAAGTGGTGCACATGATTTTGCATCTATTAGCTCTGGTATCTGTATCTTTGGGGCTTTTTGTAATCATCAAATACAAAAATGAAGTGGGAGAGACAGATTTTATTTCATTACACTCCTGGTTGGGCATAATCACCATCTCTTTATTTGGTCTTCAGTGGCTGTTTGGTTTCGTTGCTTACATCTTTCCTGGTTCAGAAATGTCAACAAGGGCATCATTTAAGCCATGGCACACTTTCTTTGGGATGGTCATTTTTCTTCTAGCAGTGTGCACTGCTGAGATAGGGCTTAATTACTTCGTCTTAGGGATCAACCAAGAAGGACTTATAGTCAACTTCACTGGCCTTCTGATCTTCTTGTTTGCAGCTGCAGTCTCCTTCACTGTCCTCCTCCCTCGACTATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

218

Amino Acids

24.32

Weight (kDa)

9.44

Isoelectric Point (pI)

25.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 54 - 181 3.8e-43 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017659)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33090
malus_domestica MD13G1016700.v1.1 MD16G1014600.v1.1
prunus_persica Prupe.1G336700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0442141
rosa_laevigata RLG00000006037
rosa_roxburghii Rroxscaffold_5G00383050
rosa_rugosa Rorug04G0339200
rosa_samantha Rh4AG390800 Rh4BG404300 Rh4CG419300 Rh4DG397300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 57
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 1 cut(s) 114
AclWI GGATC 1 cut(s) 566
AcsI RAATTY 1 cut(s) 150
AcuI CTGAAG 1 cut(s) 389
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 105
AgsI TTSAA 2 cut(s) 130, 157
AhdI GACNNNNNGTC 1 cut(s) 578
AjnI CCWGG 3 cut(s) 232, 368, 439
AluBI AGCT 5 cut(s) 21, 74, 209, 284, 617
AluI AGCT 5 cut(s) 21, 74, 209, 284, 617
Alw21I GWGCWC 2 cut(s) 264, 524
Alw26I GTCTC 2 cut(s) 338, 628
Alw44I GTGCAC 2 cut(s) 260, 520
AlwI GGATC 1 cut(s) 566
AlwNI CAGNNNCTG 1 cut(s) 412
AoxI GGCC 1 cut(s) 592
ApaLI GTGCAC 2 cut(s) 260, 520
ApeKI GCWGC 2 cut(s) 614, 617
ApoI RAATTY 1 cut(s) 150
AspLEI GCGC 3 cut(s) 7, 58, 114
AsuHPI GGTGA 1 cut(s) 376
BaeGI GKGCMC 2 cut(s) 264, 524
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 395
Bbv12I GWGCWC 2 cut(s) 264, 524
BbvI GCAGC 2 cut(s) 604, 626
BccI CCATC 2 cut(s) 395, 490
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BciT130I CCWGG 3 cut(s) 234, 370, 441
BciVI GTATCC 1 cut(s) 238
BcoDI GTCTC 2 cut(s) 338, 628
BfaI CTAG 1 cut(s) 512
BfmI CTRYAG 1 cut(s) 618
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 238
BisI GCNGC 2 cut(s) 615, 618
BlsI GCNGC 2 cut(s) 616, 619
Bme1390I CCNGG 3 cut(s) 234, 370, 441
BmeRI GACNNNNNGTC 1 cut(s) 578
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 3 cut(s) 234, 370, 441
BmrI ACTGGG 1 cut(s) 35
BmsI GCATC 2 cut(s) 283, 473
BmuI ACTGGG 1 cut(s) 35
BpiI GAAGAC 1 cut(s) 395
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 232, 476
Bse1I ACTGG 2 cut(s) 41, 595
BseBI CCWGG 3 cut(s) 234, 370, 441
BseDI CCNNGG 2 cut(s) 232, 476
BseGI GGATG 2 cut(s) 163, 501
BseMII CTCAG 1 cut(s) 519
BseNI ACTGG 2 cut(s) 41, 595
BseRI GAGGAG 2 cut(s) 216, 629
BseSI GKGCMC 2 cut(s) 264, 524
BseXI GCAGC 2 cut(s) 604, 626
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 1 cut(s) 594
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 2 cut(s) 264, 524
BsmAI GTCTC 2 cut(s) 338, 628
BsnI GGCC 1 cut(s) 594
Bsp1286I GDGCHC 2 cut(s) 264, 524
Bsp143I GATC 3 cut(s) 195, 558, 600
Bsp19I CCATGG 1 cut(s) 476
BspANI GGCC 1 cut(s) 594
BspCNI CTCAG 1 cut(s) 520
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 622
BspPI GGATC 1 cut(s) 566
BspT107I GGYRCC 1 cut(s) 4
BsrI ACTGG 2 cut(s) 41, 595
BssECI CCNNGG 2 cut(s) 232, 476
BssMI GATC 3 cut(s) 195, 558, 600
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 476
Bst2UI CCWGG 3 cut(s) 234, 370, 441
Bst4CI ACNGT 1 cut(s) 634
Bst6I CTCTTC 1 cut(s) 139
BstACI GRCGYC 1 cut(s) 5
BstDEI CTNAG 2 cut(s) 528, 553
BstDSI CCRYGG 1 cut(s) 476
BstF5I GGATG 2 cut(s) 163, 501
BstFNI CGCG 1 cut(s) 114
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 58, 114
BstKTI GATC 3 cut(s) 198, 561, 603
BstMAI GTCTC 2 cut(s) 338, 628
BstMBI GATC 3 cut(s) 195, 558, 600
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstNI CCWGG 3 cut(s) 234, 370, 441
BstSCI CCNGG 3 cut(s) 232, 368, 439
BstSFI CTRYAG 1 cut(s) 618
BstSLI GKGCMC 2 cut(s) 264, 524
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 2 cut(s) 604, 626
BstV2I GAAGAC 1 cut(s) 395
BsuI GTATCC 1 cut(s) 238
BsuRI GGCC 1 cut(s) 594
BtgI CCRYGG 1 cut(s) 476
BtsCI GGATG 2 cut(s) 163, 501
BtsI GCAGTG 2 cut(s) 522, 522
BtsIMutI CAGTG 5 cut(s) 413, 522, 522, 588, 630
CaiI CAGNNNCTG 1 cut(s) 412
CfoI GCGC 3 cut(s) 7, 58, 114
Csp6I GTAC 1 cut(s) 104
CviAII CATG 2 cut(s) 265, 477
CviQI GTAC 1 cut(s) 104
DdeI CTNAG 2 cut(s) 528, 553
DinI GGCGCC 1 cut(s) 6
DpnI GATC 3 cut(s) 197, 560, 602
DpnII GATC 3 cut(s) 195, 558, 600
DriI GACNNNNNGTC 1 cut(s) 578
Eam1104I CTCTTC 1 cut(s) 139
Eam1105I GACNNNNNGTC 1 cut(s) 578
EarI CTCTTC 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 476
Eco57I CTGAAG 1 cut(s) 389
EcoRII CCWGG 3 cut(s) 232, 368, 439
EcoT14I CCWWGG 1 cut(s) 476
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 476
FaeI CATG 2 cut(s) 268, 480
FalI AAGNNNNNCTT 2 cut(s) 558, 590
FatI CATG 2 cut(s) 264, 476
Fnu4HI GCNGC 2 cut(s) 615, 618
FokI GGATG 2 cut(s) 150, 508
Fsp4HI GCNGC 2 cut(s) 615, 618
FspBI CTAG 1 cut(s) 512
FspI TGCGCA 1 cut(s) 57
GlaI GCGC 3 cut(s) 6, 57, 113
GluI GCNGC 2 cut(s) 615, 618
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 594
HhaI GCGC 3 cut(s) 7, 58, 114
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 268, 480
Hin6I GCGC 3 cut(s) 5, 56, 112
HinP1I GCGC 3 cut(s) 5, 56, 112
HincII GTYRAC 2 cut(s) 456, 583
HindII GTYRAC 2 cut(s) 456, 583
HinfI GANTC 1 cut(s) 13
HphI GGTGA 1 cut(s) 376
Hpy166II GTNNAC 5 cut(s) 106, 262, 456, 522, 583
Hpy188I TCNGA 3 cut(s) 200, 448, 600
Hpy8I GTNNAC 5 cut(s) 106, 262, 456, 522, 583
HpyAV CCTTC 5 cut(s) 95, 110, 563, 605, 637
HpyCH4III ACNGT 1 cut(s) 634
HpyCH4V TGCA 5 cut(s) 262, 274, 522, 614, 620
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
HpyF3I CTNAG 2 cut(s) 528, 553
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 268, 480
HspAI GCGC 3 cut(s) 5, 56, 112
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 3 cut(s) 195, 558, 600
LpnPI CCDG 9 cut(s) 54, 219, 246, 272, 355, 382, 426, 453, 576
Lsp1109I GCAGC 2 cut(s) 604, 626
LweI GCATC 2 cut(s) 283, 473
MaeI CTAG 1 cut(s) 512
MaeIII GTNAC 2 cut(s) 43, 63
MalI GATC 3 cut(s) 197, 560, 602
MboI GATC 3 cut(s) 195, 558, 600
MboII GAAGA 5 cut(s) 126, 152, 395, 500, 595
MhlI GDGCHC 2 cut(s) 264, 524
MluCI AATT 3 cut(s) 51, 150, 541
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 22
MnlI CCTC 4 cut(s) 194, 647, 650, 654
MseI TTAA 3 cut(s) 471, 540, 655
MspA1I CMGCKG 1 cut(s) 617
MspR9I CCNGG 3 cut(s) 234, 370, 441
MvaI CCWGG 3 cut(s) 234, 370, 441
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 1 cut(s) 215
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 1 cut(s) 476
NdeII GATC 3 cut(s) 195, 558, 600
NlaIII CATG 2 cut(s) 268, 480
NlaIV GGNNCC 1 cut(s) 6
NsbI TGCGCA 1 cut(s) 57
PkrI GCNGC 2 cut(s) 616, 619
PleI GAGTC 1 cut(s) 21
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 21
Psp6I CCWGG 3 cut(s) 232, 368, 439
PspGI CCWGG 3 cut(s) 232, 368, 439
PspN4I GGNNCC 1 cut(s) 6
PstI CTGCAG 1 cut(s) 622
PstNI CAGNNNCTG 1 cut(s) 412
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 1 cut(s) 105
RsaNI GTAC 1 cut(s) 104
SaqAI TTAA 3 cut(s) 471, 540, 655
SatI GCNGC 2 cut(s) 615, 618
Sau3AI GATC 3 cut(s) 195, 558, 600
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 3 cut(s) 234, 370, 441
SduI GDGCHC 2 cut(s) 264, 524
SetI ASST 5 cut(s) 23, 76, 211, 286, 619
SfaNI GCATC 2 cut(s) 283, 473
SfcI CTRYAG 1 cut(s) 618
SfoI GGCGCC 1 cut(s) 6
Sse9I AATT 3 cut(s) 51, 150, 541
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 1 cut(s) 512
StyD4I CCNGG 3 cut(s) 232, 368, 439
StyI CCWWGG 1 cut(s) 476
TaaI ACNGT 1 cut(s) 634
TaqI TCGA 1 cut(s) 646
TasI AATT 3 cut(s) 51, 150, 541
Tru1I TTAA 3 cut(s) 471, 540, 655
Tru9I TTAA 3 cut(s) 471, 540, 655
TscAI CASTG 5 cut(s) 413, 522, 529, 595, 637
TseI GCWGC 2 cut(s) 614, 617
TspDTI ATGAA 3 cut(s) 152, 348, 348
TspGWI ACGGA 1 cut(s) 156
TspRI CASTG 5 cut(s) 413, 522, 529, 595, 637
VneI GTGCAC 2 cut(s) 260, 520
XapI RAATTY 1 cut(s) 150
XspI CTAG 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.