MD16G1014600.v1.1

ascorbate-specific transmembrane electron transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0017659
Physical Location & Seq
Forward (+)
1127680 .. 1129390
1711 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1014600.v1.1.491

Sequence Viewer

Length: 642 bp
ATGATGGTGTACACGATATTTTACTGTGCAAACGTCATAATCGGAACCATTCATTTTCTTTATGATAATCTAAAGATAAATGTTGTACCCAGTGCACAAGGCTCCCGCTTTACTCAGGGTCTGGAAGAGGTTCATACGTTTCTAATGGTGGTCGGGTTTATTTTGATCGGTGGAGAAGCAATCATGGCATACAAGACAGTCCAAGGGACAAGGAACACACAAAAAGGGGTGCACGTGATATTGCATCTTCTAGCTCTGGCGGCTATAATTTTTGGGTTTTACTGTGCTATCCAGTTTAATCACGAATCCAGAGTTCCACATTTTCTTACCTTACACTCCTGGCTCGGCATAACAACCATCTCTTTATTTGGTATTCAGTACTTGTTTGGCTTCGTGTTCTATGTGTTTCCGGGTGGAGACATGTCGTCGAGGGGAAATTTTATGCCATGGCACACATTTTTTGGGATGGTGATATTTCTTCTAGCAGTGTGCACGGCTGAGACAGGGTTAATGGAGAGGTTCTTTTTCTTGGCCGTAGTAAAAATTAATCAAGAAGTTCTTATAGTCAACTTCACTGGTCTTTTGCTCTTTCTCTTCGCAGCCACCGTCAGCCTCACCGTCCTCCTCCCTCGGCTTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

214

Amino Acids

24.0

Weight (kDa)

7.81

Isoelectric Point (pI)

27.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 44 - 176 7.3e-41 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017659)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33090
malus_domestica MD13G1016700.v1.1 MD16G1014600.v1.1
prunus_persica Prupe.1G336700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0442141
rosa_laevigata RLG00000006037
rosa_roxburghii Rroxscaffold_5G00383050
rosa_rugosa Rorug04G0339200
rosa_samantha Rh4AG390800 Rh4BG404300 Rh4CG419300 Rh4DG397300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 106, 260
AcoI YGGCCR 1 cut(s) 531
AcsI RAATTY 1 cut(s) 436
AcvI CACGTG 1 cut(s) 235
AfaI GTAC 3 cut(s) 11, 87, 380
AflIII ACRYGT 1 cut(s) 420
AhdI GACNNNNNGTC 1 cut(s) 424
AjnI CCWGG 1 cut(s) 338
AluBI AGCT 1 cut(s) 254
AluI AGCT 1 cut(s) 254
Alw21I GWGCWC 3 cut(s) 97, 234, 494
Alw26I GTCTC 2 cut(s) 411, 494
Alw44I GTGCAC 3 cut(s) 93, 230, 490
AlwNI CAGNNNCTG 1 cut(s) 121
AoxI GGCC 1 cut(s) 531
ApaLI GTGCAC 3 cut(s) 93, 230, 490
ApeKI GCWGC 1 cut(s) 599
ApoI RAATTY 1 cut(s) 436
AseI ATTAAT 1 cut(s) 546
Asp700I GAANNNNTTC 1 cut(s) 129
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 2 cut(s) 481, 607
BaeGI GKGCMC 3 cut(s) 97, 234, 494
BbrPI CACGTG 1 cut(s) 235
Bbv12I GWGCWC 3 cut(s) 97, 234, 494
BbvI GCAGC 1 cut(s) 611
BccI CCATC 2 cut(s) 365, 460
BceAI ACGGC 2 cut(s) 510, 518
BciT130I CCWGG 1 cut(s) 340
BcnI CCSGG 1 cut(s) 411
BcoDI GTCTC 2 cut(s) 411, 494
BfaI CTAG 2 cut(s) 251, 482
BisI GCNGC 2 cut(s) 261, 600
BlsI GCNGC 2 cut(s) 262, 601
BmcAI AGTACT 1 cut(s) 380
Bme1390I CCNGG 2 cut(s) 340, 411
BmeRI GACNNNNNGTC 1 cut(s) 424
BmiI GGNNCC 2 cut(s) 46, 103
BmrFI CCNGG 2 cut(s) 340, 411
BmrI ACTGGG 1 cut(s) 84
BmsI GCATC 1 cut(s) 253
BmuI ACTGGG 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 411
BsaAI YACGTR 1 cut(s) 235
BsaJI CCNNGG 3 cut(s) 202, 446, 629
Bse1I ACTGG 3 cut(s) 90, 292, 580
BseBI CCWGG 1 cut(s) 340
BseDI CCNNGG 3 cut(s) 202, 446, 629
BseGI GGATG 1 cut(s) 471
BseMII CTCAG 2 cut(s) 128, 489
BseNI ACTGG 3 cut(s) 90, 292, 580
BseRI GAGGAG 1 cut(s) 614
BseSI GKGCMC 3 cut(s) 97, 234, 494
BseXI GCAGC 1 cut(s) 611
BshFI GGCC 1 cut(s) 533
BsiHKAI GWGCWC 3 cut(s) 97, 234, 494
BsiSI CCGG 1 cut(s) 410
BslFI GGGAC 1 cut(s) 220
BsmAI GTCTC 2 cut(s) 411, 494
BsmFI GGGAC 1 cut(s) 220
BsnI GGCC 1 cut(s) 533
Bsp1286I GDGCHC 3 cut(s) 97, 234, 494
Bsp1407I TGTACA 1 cut(s) 9
Bsp143I GATC 1 cut(s) 165
Bsp19I CCATGG 1 cut(s) 446
BspACI CCGC 2 cut(s) 106, 260
BspANI GGCC 1 cut(s) 533
BspCNI CTCAG 2 cut(s) 127, 490
BspLI GGNNCC 2 cut(s) 46, 103
BsrGI TGTACA 1 cut(s) 9
BsrI ACTGG 3 cut(s) 90, 292, 580
BssECI CCNNGG 3 cut(s) 202, 446, 629
BssMI GATC 1 cut(s) 165
BssT1I CCWWGG 2 cut(s) 202, 446
Bst2UI CCWGG 1 cut(s) 340
Bst4CI ACNGT 5 cut(s) 26, 199, 284, 607, 619
Bst6I CTCTTC 2 cut(s) 120, 599
BstAUI TGTACA 1 cut(s) 9
BstBAI YACGTR 1 cut(s) 235
BstDEI CTNAG 2 cut(s) 114, 498
BstDSI CCRYGG 1 cut(s) 446
BstF5I GGATG 1 cut(s) 471
BstKTI GATC 1 cut(s) 168
BstMAI GTCTC 2 cut(s) 411, 494
BstMBI GATC 1 cut(s) 165
BstMWI GCNNNNNNNGC 2 cut(s) 185, 260
BstNI CCWGG 1 cut(s) 340
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 2 cut(s) 338, 409
BstSLI GKGCMC 3 cut(s) 97, 234, 494
BstV1I GCAGC 1 cut(s) 611
BsuRI GGCC 1 cut(s) 533
BtgI CCRYGG 1 cut(s) 446
BtsCI GGATG 1 cut(s) 471
BtsI GCAGTG 1 cut(s) 492
BtsIMutI CAGTG 3 cut(s) 97, 492, 573
CaiI CAGNNNCTG 1 cut(s) 121
Csp6I GTAC 3 cut(s) 10, 86, 379
CviAII CATG 3 cut(s) 184, 421, 447
CviQI GTAC 3 cut(s) 10, 86, 379
DdeI CTNAG 2 cut(s) 114, 498
DpnI GATC 1 cut(s) 167
DpnII GATC 1 cut(s) 165
DriI GACNNNNNGTC 1 cut(s) 424
EaeI YGGCCR 1 cut(s) 531
Eam1104I CTCTTC 2 cut(s) 120, 599
Eam1105I GACNNNNNGTC 1 cut(s) 424
EarI CTCTTC 2 cut(s) 120, 599
Eco130I CCWWGG 2 cut(s) 202, 446
Eco72I CACGTG 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 338
EcoT14I CCWWGG 2 cut(s) 202, 446
ErhI CCWWGG 2 cut(s) 202, 446
FaeI CATG 3 cut(s) 187, 424, 450
FalI AAGNNNNNCTT 2 cut(s) 543, 575
FaqI GGGAC 1 cut(s) 220
FatI CATG 3 cut(s) 183, 420, 446
FauI CCCGC 1 cut(s) 113
Fnu4HI GCNGC 2 cut(s) 261, 600
FokI GGATG 1 cut(s) 478
Fsp4HI GCNGC 2 cut(s) 261, 600
FspBI CTAG 2 cut(s) 251, 482
GluI GCNGC 2 cut(s) 261, 600
HaeIII GGCC 1 cut(s) 533
HapII CCGG 1 cut(s) 410
Hin1II CATG 3 cut(s) 187, 424, 450
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HinfI GANTC 1 cut(s) 305
HpaII CCGG 1 cut(s) 410
HphI GGTGA 2 cut(s) 481, 607
Hpy166II GTNNAC 6 cut(s) 10, 12, 95, 232, 492, 568
Hpy188I TCNGA 1 cut(s) 44
Hpy188III TCNNGA 4 cut(s) 122, 302, 309, 551
Hpy8I GTNNAC 6 cut(s) 10, 12, 95, 232, 492, 568
Hpy99I CGWCG 1 cut(s) 430
HpyCH4III ACNGT 5 cut(s) 26, 199, 284, 607, 619
HpyCH4IV ACGT 3 cut(s) 33, 137, 234
HpyCH4V TGCA 5 cut(s) 29, 95, 232, 244, 492
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 260
HpyF3I CTNAG 2 cut(s) 114, 498
HpySE526I ACGT 3 cut(s) 33, 137, 234
Hsp92II CATG 3 cut(s) 187, 424, 450
Kzo9I GATC 1 cut(s) 165
LmnI GCTCC 1 cut(s) 107
Lsp1109I GCAGC 1 cut(s) 611
LweI GCATC 1 cut(s) 253
MaeI CTAG 2 cut(s) 251, 482
MaeII ACGT 3 cut(s) 33, 137, 234
MalI GATC 1 cut(s) 167
MboI GATC 1 cut(s) 165
MboII GAAGA 4 cut(s) 137, 239, 470, 586
MhlI GDGCHC 3 cut(s) 97, 234, 494
MluCI AATT 3 cut(s) 267, 436, 543
MnlI CCTC 7 cut(s) 121, 423, 510, 623, 632, 635, 639
MroXI GAANNNNTTC 1 cut(s) 129
MseI TTAA 4 cut(s) 297, 509, 546, 640
MspI CCGG 1 cut(s) 410
MspR9I CCNGG 2 cut(s) 340, 411
MvaI CCWGG 1 cut(s) 340
MwoI GCNNNNNNNGC 2 cut(s) 185, 260
NciI CCSGG 1 cut(s) 411
NcoI CCATGG 1 cut(s) 446
NdeII GATC 1 cut(s) 165
NlaIII CATG 3 cut(s) 187, 424, 450
NlaIV GGNNCC 2 cut(s) 46, 103
NmeAIII GCCGAG 2 cut(s) 324, 610
NspI RCATGY 1 cut(s) 424
PciI ACATGT 1 cut(s) 420
PcsI WCGNNNNNNNCGW 1 cut(s) 603
PdmI GAANNNNTTC 1 cut(s) 129
PfeI GAWTC 1 cut(s) 305
PkrI GCNGC 2 cut(s) 262, 601
PmaCI CACGTG 1 cut(s) 235
PmlI CACGTG 1 cut(s) 235
Ppu21I YACGTR 1 cut(s) 235
PscI ACATGT 1 cut(s) 420
PshBI ATTAAT 1 cut(s) 546
Psp6I CCWGG 1 cut(s) 338
PspCI CACGTG 1 cut(s) 235
PspGI CCWGG 1 cut(s) 338
PspN4I GGNNCC 2 cut(s) 46, 103
PstNI CAGNNNCTG 1 cut(s) 121
RsaI GTAC 3 cut(s) 11, 87, 380
RsaNI GTAC 3 cut(s) 10, 86, 379
SaqAI TTAA 4 cut(s) 297, 509, 546, 640
SatI GCNGC 2 cut(s) 261, 600
Sau3AI GATC 1 cut(s) 165
ScaI AGTACT 1 cut(s) 380
ScrFI CCNGG 2 cut(s) 340, 411
SduI GDGCHC 3 cut(s) 97, 234, 494
SetI ASST 7 cut(s) 36, 132, 140, 237, 256, 332, 521
SfaNI GCATC 1 cut(s) 253
Sse9I AATT 3 cut(s) 267, 436, 543
SsiI CCGC 2 cut(s) 106, 260
SspMI CTAG 2 cut(s) 251, 482
StyD4I CCNGG 2 cut(s) 338, 409
StyI CCWWGG 2 cut(s) 202, 446
TaaI ACNGT 5 cut(s) 26, 199, 284, 607, 619
TaiI ACGT 3 cut(s) 36, 140, 237
TaqI TCGA 1 cut(s) 428
TasI AATT 3 cut(s) 267, 436, 543
TatI WGTACW 2 cut(s) 9, 378
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 1 cut(s) 305
Tru1I TTAA 4 cut(s) 297, 509, 546, 640
Tru9I TTAA 4 cut(s) 297, 509, 546, 640
TscAI CASTG 3 cut(s) 97, 492, 580
TseI GCWGC 1 cut(s) 599
TspDTI ATGAA 2 cut(s) 41, 122
TspRI CASTG 3 cut(s) 97, 492, 580
VneI GTGCAC 3 cut(s) 93, 230, 490
VspI ATTAAT 1 cut(s) 546
XapI RAATTY 1 cut(s) 436
XceI RCATGY 1 cut(s) 424
XmnI GAANNNNTTC 1 cut(s) 129
XspI CTAG 2 cut(s) 251, 482
ZrmI AGTACT 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.