Prupe.1G336700_v2.0.a1

ascorbate-specific transmembrane electron transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0017659
Physical Location & Seq
Reverse (-)
31766385 .. 31767349
965 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G336700.1

Sequence Viewer

Length: 669 bp
ATGGCGCCGAAGAGCCGTAGCTACCAGATATCAGCAACCCCAGTTACAATATTTGTCCATTTGTTAGCTATAGCCATAACAACCCTTGTGCTTATTTGGCTCCTACACTTCCGCCATGGCCTTGCCCTCAACTCCTTCATCACAGAGAAAATTTTAAACGTTCATACGCTTCTAATGGTAATTGGGTTTATTTTGATTGGAGGAGAAGCTATCATGGCATACAAGACAGTCCCAGGGAAAAGGAACACACAGAAAATGGTGCACCTGATATTGCATTTTCTAGCTCTGGTGGCTATAATCTTGGGGATTTATGCAGCTTTCAAGTTTAATCATGAATCGGGAATTCCAAATTTGCTCACCTTACATTCCTGGCTCGGCATAATCACCATCTCTTTATTTGGTCTTCAGTGGTTGTTTGCTTTCTTTGCCTATGTGTTTCCGGGTGCAGAATCGTCAGCAAGGGGAAATCTTGTGCCATGGCACACTTTCGTGGGCATGGTCATCTTTCTGCTAGCAGTATGCACTGCCGAGGCAGGGTTGCTGGAGAGGTTCCTTTTCTTAAGCATAGGGCGCACCCAAGAAACACTTATAATCAACTTCACTGGACTTTTGATCTTTCTATTTGCAGCCAGCGTCAGCCTCTCTGTCCTCCTCCCTCGACTTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

223

Amino Acids

24.67

Weight (kDa)

9.77

Isoelectric Point (pI)

29.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017659)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33090
malus_domestica MD13G1016700.v1.1 MD16G1014600.v1.1
prunus_persica Prupe.1G336700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0442141
rosa_laevigata RLG00000006037
rosa_roxburghii Rroxscaffold_5G00383050
rosa_rugosa Rorug04G0339200
rosa_samantha Rh4AG390800 Rh4BG404300 Rh4CG419300 Rh4DG397300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 590
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 1 cut(s) 112
AclI AACGTT 1 cut(s) 159
AcsI RAATTY 3 cut(s) 150, 342, 349
AcuI CTGAAG 1 cut(s) 389
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 534
AflII CTTAAG 1 cut(s) 559
AgsI TTSAA 1 cut(s) 322
AjnI CCWGG 2 cut(s) 232, 368
AleI CACNNNNGTG 1 cut(s) 488
AluBI AGCT 5 cut(s) 21, 68, 209, 284, 317
AluI AGCT 5 cut(s) 21, 68, 209, 284, 317
Alw21I GWGCWC 1 cut(s) 264
Alw44I GTGCAC 1 cut(s) 260
AoxI GGCC 1 cut(s) 118
ApaLI GTGCAC 1 cut(s) 260
ApeKI GCWGC 2 cut(s) 314, 626
ApoI RAATTY 3 cut(s) 150, 342, 349
AspLEI GCGC 2 cut(s) 7, 573
AsuC2I CCSGG 1 cut(s) 441
AsuHPI GGTGA 2 cut(s) 349, 376
AsuNHI GCTAGC 1 cut(s) 511
BaeGI GKGCMC 1 cut(s) 264
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 2 cut(s) 326, 638
BccI CCATC 1 cut(s) 395
BciT130I CCWGG 2 cut(s) 234, 370
BcnI CCSGG 1 cut(s) 441
BfaI CTAG 2 cut(s) 281, 512
BfmI CTRYAG 1 cut(s) 69
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 559
BisI GCNGC 2 cut(s) 315, 627
BlsI GCNGC 2 cut(s) 316, 628
Bme1390I CCNGG 3 cut(s) 234, 370, 441
BmiI GGNNCC 3 cut(s) 6, 101, 551
BmrFI CCNGG 3 cut(s) 234, 370, 441
BmrI ACTGGG 1 cut(s) 35
BmtI GCTAGC 1 cut(s) 515
BmuI ACTGGG 1 cut(s) 35
BpiI GAAGAC 1 cut(s) 395
BpmI CTGGAG 1 cut(s) 563
BpuMI CCSGG 1 cut(s) 441
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 5 cut(s) 115, 232, 233, 476, 528
Bsc4I CCNNNNNNNGG 1 cut(s) 534
Bse1I ACTGG 2 cut(s) 41, 607
BseBI CCWGG 2 cut(s) 234, 370
BseDI CCNNGG 5 cut(s) 115, 232, 233, 476, 528
BseLI CCNNNNNNNGG 1 cut(s) 534
BseNI ACTGG 2 cut(s) 41, 607
BseRI GAGGAG 2 cut(s) 216, 641
BseSI GKGCMC 1 cut(s) 264
BseXI GCAGC 2 cut(s) 326, 638
BsgI GTGCAG 1 cut(s) 465
BshFI GGCC 1 cut(s) 120
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 264
BsiSI CCGG 1 cut(s) 440
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 1 cut(s) 534
BsmFI GGGAC 1 cut(s) 215
BsnI GGCC 1 cut(s) 120
Bsp1286I GDGCHC 1 cut(s) 264
Bsp143I GATC 1 cut(s) 612
Bsp19I CCATGG 2 cut(s) 115, 476
BspACI CCGC 1 cut(s) 112
BspANI GGCC 1 cut(s) 120
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 3 cut(s) 6, 101, 551
BspOI GCTAGC 1 cut(s) 515
BspQI GCTCTTC 1 cut(s) 5
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 559
BsrI ACTGG 2 cut(s) 41, 607
BssECI CCNNGG 5 cut(s) 115, 232, 233, 476, 528
BssMI GATC 1 cut(s) 612
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 115, 476
Bst2UI CCWGG 2 cut(s) 234, 370
Bst4CI ACNGT 1 cut(s) 229
Bst6I CTCTTC 1 cut(s) 5
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 559
BstC8I GCNNGC 2 cut(s) 513, 631
BstDSI CCRYGG 2 cut(s) 115, 476
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 573
BstKTI GATC 1 cut(s) 615
BstMBI GATC 1 cut(s) 612
BstMWI GCNNNNNNNGC 5 cut(s) 97, 215, 290, 425, 570
BstNI CCWGG 2 cut(s) 234, 370
BstSCI CCNGG 3 cut(s) 232, 368, 439
BstSFI CTRYAG 1 cut(s) 69
BstSLI GKGCMC 1 cut(s) 264
BstV1I GCAGC 2 cut(s) 326, 638
BstV2I GAAGAC 1 cut(s) 395
BsuRI GGCC 1 cut(s) 120
BtgI CCRYGG 2 cut(s) 115, 476
BtsI GCAGTG 1 cut(s) 522
BtsIMutI CAGTG 3 cut(s) 413, 522, 600
Cac8I GCNNGC 2 cut(s) 513, 631
CciI TCATGA 1 cut(s) 331
CfoI GCGC 2 cut(s) 7, 573
CseI GACGC 1 cut(s) 622
CviAII CATG 5 cut(s) 116, 214, 332, 477, 496
DinI GGCGCC 1 cut(s) 6
DpnI GATC 1 cut(s) 614
DpnII GATC 1 cut(s) 612
DraI TTTAAA 1 cut(s) 156
Eam1104I CTCTTC 1 cut(s) 5
EarI CTCTTC 1 cut(s) 5
EciI GGCGGA 1 cut(s) 101
Eco130I CCWWGG 2 cut(s) 115, 476
Eco32I GATATC 1 cut(s) 30
Eco57I CTGAAG 1 cut(s) 389
EcoRI GAATTC 1 cut(s) 342
EcoRII CCWGG 2 cut(s) 232, 368
EcoRV GATATC 1 cut(s) 30
EcoT14I CCWWGG 2 cut(s) 115, 476
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 115, 476
FaeI CATG 5 cut(s) 119, 217, 335, 480, 499
FalI AAGNNNNNCTT 2 cut(s) 570, 602
FaqI GGGAC 1 cut(s) 215
FatI CATG 5 cut(s) 115, 213, 331, 476, 495
Fnu4HI GCNGC 2 cut(s) 315, 627
Fsp4HI GCNGC 2 cut(s) 315, 627
FspBI CTAG 2 cut(s) 281, 512
GlaI GCGC 2 cut(s) 6, 572
GluI GCNGC 2 cut(s) 315, 627
GsuI CTGGAG 1 cut(s) 563
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 120
HapII CCGG 1 cut(s) 440
HgaI GACGC 1 cut(s) 622
HhaI GCGC 2 cut(s) 7, 573
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 119, 217, 335, 480, 499
Hin6I GCGC 2 cut(s) 5, 571
HinP1I GCGC 2 cut(s) 5, 571
HinfI GANTC 2 cut(s) 335, 449
HpaII CCGG 1 cut(s) 440
HphI GGTGA 2 cut(s) 349, 376
Hpy166II GTNNAC 1 cut(s) 262
Hpy188III TCNNGA 2 cut(s) 332, 339
Hpy8I GTNNAC 1 cut(s) 262
HpyAV CCTTC 1 cut(s) 145
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 6 cut(s) 262, 274, 314, 446, 522, 626
HpyF10VI GCNNNNNNNGC 5 cut(s) 97, 215, 290, 425, 570
HpySE526I ACGT 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 119, 217, 335, 480, 499
HspAI GCGC 2 cut(s) 5, 571
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 1 cut(s) 612
LguI GCTCTTC 1 cut(s) 5
LmnI GCTCC 1 cut(s) 105
Lsp1109I GCAGC 2 cut(s) 326, 638
MaeI CTAG 2 cut(s) 281, 512
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 1 cut(s) 43
MalI GATC 1 cut(s) 614
MboI GATC 1 cut(s) 612
MboII GAAGA 2 cut(s) 22, 395
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 4 cut(s) 150, 180, 342, 349
Mly113I GGCGCC 1 cut(s) 5
MnlI CCTC 8 cut(s) 137, 194, 523, 540, 650, 659, 662, 666
MseI TTAA 3 cut(s) 155, 327, 560
MslI CAYNNNNRTG 1 cut(s) 488
MspCI CTTAAG 1 cut(s) 559
MspI CCGG 1 cut(s) 440
MspR9I CCNGG 3 cut(s) 234, 370, 441
MvaI CCWGG 2 cut(s) 234, 370
MwoI GCNNNNNNNGC 5 cut(s) 97, 215, 290, 425, 570
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 441
NcoI CCATGG 2 cut(s) 115, 476
NdeII GATC 1 cut(s) 612
NheI GCTAGC 1 cut(s) 511
NlaIII CATG 5 cut(s) 119, 217, 335, 480, 499
NlaIV GGNNCC 3 cut(s) 6, 101, 551
NmeAIII GCCGAG 2 cut(s) 354, 553
OliI CACNNNNGTG 1 cut(s) 488
PagI TCATGA 1 cut(s) 331
PasI CCCWGGG 1 cut(s) 233
PciSI GCTCTTC 1 cut(s) 5
PfeI GAWTC 2 cut(s) 335, 449
PkrI GCNGC 2 cut(s) 316, 628
PluTI GGCGCC 1 cut(s) 8
PsiI TTATAA 1 cut(s) 590
Psp1406I AACGTT 1 cut(s) 159
Psp6I CCWGG 2 cut(s) 232, 368
PspGI CCWGG 2 cut(s) 232, 368
PspN4I GGNNCC 3 cut(s) 6, 101, 551
RseI CAYNNNNRTG 1 cut(s) 488
SapI GCTCTTC 1 cut(s) 5
SaqAI TTAA 3 cut(s) 155, 327, 560
SatI GCNGC 2 cut(s) 315, 627
Sau3AI GATC 1 cut(s) 612
ScrFI CCNGG 3 cut(s) 234, 370, 441
SduI GDGCHC 1 cut(s) 264
SetI ASST 9 cut(s) 23, 70, 162, 211, 267, 286, 319, 362, 551
SfcI CTRYAG 1 cut(s) 69
SfoI GGCGCC 1 cut(s) 6
SmiMI CAYNNNNRTG 1 cut(s) 488
SmlI CTYRAG 1 cut(s) 559
SmoI CTYRAG 1 cut(s) 559
Sse9I AATT 4 cut(s) 150, 180, 342, 349
SsiI CCGC 1 cut(s) 112
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 51
SspMI CTAG 2 cut(s) 281, 512
StyD4I CCNGG 3 cut(s) 232, 368, 439
StyI CCWWGG 2 cut(s) 115, 476
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 1 cut(s) 162
TaqI TCGA 1 cut(s) 658
TasI AATT 4 cut(s) 150, 180, 342, 349
TfiI GAWTC 2 cut(s) 335, 449
Tru1I TTAA 3 cut(s) 155, 327, 560
Tru9I TTAA 3 cut(s) 155, 327, 560
TscAI CASTG 3 cut(s) 413, 529, 607
TseI GCWGC 2 cut(s) 314, 626
TspDTI ATGAA 3 cut(s) 127, 152, 348
TspRI CASTG 3 cut(s) 413, 529, 607
Vha464I CTTAAG 1 cut(s) 559
VneI GTGCAC 1 cut(s) 260
XapI RAATTY 3 cut(s) 150, 342, 349
XspI CTAG 2 cut(s) 281, 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.