MD13G1016700.v1.1

ascorbate-specific transmembrane electron transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0017659
Physical Location & Seq
Forward (+)
1068798 .. 1070545
1748 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1016700.v1.1.491

Sequence Viewer

Length: 468 bp
ATGTCAATCATGGCATACAAGACAGTCCAAGGGGAAAGGAACACGCAAAAAGGGGCGCACCTGATATTGCATCTTCTAGCTCTGGTGGCTATAATTTTTGGGTTTTACTGTGCCATCAAGTTTGATCACGAATCCGGAGTTCAACATTTTCTTACCTTACATTCCTGGCTCGGCATAATCACCATCTCGTTATTTGGTCTTCAGTACTTGTTTGGCTTCGTGCTGTATGTGTTTCCGGGTGGCGACAAGTCGTCGAGGGGAAATTTTATGCCATGGCACACATTTTTCGGGATGGTGGTATTTCTGCTAGCAGTGTGCACGGCTGAGACAGGGTTGCTGGAGAGGTTCCTTTTCTTGGGCGTAATAAAAATGAATGAACAAGTACTGATAGTCAACTTCATTGGCCTTTTGCTCTTTCTCTTTGCAGCCACCGTCAGCCTCACCGCCCTCCTCCCTGGGCCTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

156

Amino Acids

17.33

Weight (kDa)

7.85

Isoelectric Point (pI)

27.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 2 - 118 2.8e-34 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017659)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33090
malus_domestica MD13G1016700.v1.1 MD16G1014600.v1.1
prunus_persica Prupe.1G336700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0442141
rosa_laevigata RLG00000006037
rosa_roxburghii Rroxscaffold_5G00383050
rosa_rugosa Rorug04G0339200
rosa_samantha Rh4AG390800 Rh4BG404300 Rh4CG419300 Rh4DG397300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 134
AciI CCGC 1 cut(s) 444
AcsI RAATTY 1 cut(s) 262
AcuI CTGAAG 1 cut(s) 185
AfaI GTAC 2 cut(s) 206, 384
AfiI CCNNNNNNNGG 1 cut(s) 355
AgsI TTSAA 1 cut(s) 143
AhdI GACNNNNNGTC 1 cut(s) 250
AjnI CCWGG 2 cut(s) 164, 454
AluBI AGCT 1 cut(s) 80
AluI AGCT 1 cut(s) 80
Alw21I GWGCWC 1 cut(s) 320
Alw26I GTCTC 1 cut(s) 320
Alw44I GTGCAC 1 cut(s) 316
Aor13HI TCCGGA 1 cut(s) 134
AoxI GGCC 2 cut(s) 403, 458
ApaLI GTGCAC 1 cut(s) 316
ApeKI GCWGC 1 cut(s) 425
ApoI RAATTY 1 cut(s) 262
AspLEI GCGC 1 cut(s) 58
AspS9I GGNCC 1 cut(s) 458
AsuC2I CCSGG 1 cut(s) 237
AsuHPI GGTGA 2 cut(s) 172, 433
AsuNHI GCTAGC 1 cut(s) 307
BaeGI GKGCMC 1 cut(s) 320
BbsI GAAGAC 1 cut(s) 191
Bbv12I GWGCWC 1 cut(s) 320
BbvI GCAGC 1 cut(s) 437
BccI CCATC 3 cut(s) 122, 191, 286
BceAI ACGGC 1 cut(s) 336
BciT130I CCWGG 2 cut(s) 166, 456
BclI TGATCA 1 cut(s) 124
BcnI CCSGG 1 cut(s) 237
BcoDI GTCTC 1 cut(s) 320
BfaI CTAG 2 cut(s) 77, 308
BisI GCNGC 1 cut(s) 426
BlsI GCNGC 1 cut(s) 427
BmcAI AGTACT 2 cut(s) 206, 384
Bme1390I CCNGG 3 cut(s) 166, 237, 456
BmeRI GACNNNNNGTC 1 cut(s) 250
BmgT120I GGNCC 1 cut(s) 458
BmiI GGNNCC 1 cut(s) 347
BmrFI CCNGG 3 cut(s) 166, 237, 456
BmsI GCATC 1 cut(s) 79
BmtI GCTAGC 1 cut(s) 311
BpiI GAAGAC 1 cut(s) 191
BpmI CTGGAG 1 cut(s) 359
BpuMI CCSGG 1 cut(s) 237
BsaJI CCNNGG 4 cut(s) 28, 272, 454, 455
BsaWI WCCGGW 1 cut(s) 134
Bsc4I CCNNNNNNNGG 1 cut(s) 355
BseAI TCCGGA 1 cut(s) 134
BseBI CCWGG 2 cut(s) 166, 456
BseDI CCNNGG 4 cut(s) 28, 272, 454, 455
BseGI GGATG 1 cut(s) 297
BseLI CCNNNNNNNGG 1 cut(s) 355
BseMII CTCAG 1 cut(s) 315
BseRI GAGGAG 1 cut(s) 440
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 1 cut(s) 437
BshFI GGCC 2 cut(s) 405, 460
BsiHKAI GWGCWC 1 cut(s) 320
BsiSI CCGG 2 cut(s) 135, 236
BslI CCNNNNNNNGG 1 cut(s) 355
BsmAI GTCTC 1 cut(s) 320
BsnI GGCC 2 cut(s) 405, 460
Bsp1286I GDGCHC 1 cut(s) 320
Bsp13I TCCGGA 1 cut(s) 134
Bsp143I GATC 1 cut(s) 124
Bsp19I CCATGG 1 cut(s) 272
BspACI CCGC 1 cut(s) 444
BspANI GGCC 2 cut(s) 405, 460
BspCNI CTCAG 1 cut(s) 316
BspEI TCCGGA 1 cut(s) 134
BspLI GGNNCC 1 cut(s) 347
BspOI GCTAGC 1 cut(s) 311
BssECI CCNNGG 4 cut(s) 28, 272, 454, 455
BssMI GATC 1 cut(s) 124
BssT1I CCWWGG 2 cut(s) 28, 272
Bst2UI CCWGG 2 cut(s) 166, 456
Bst4CI ACNGT 3 cut(s) 25, 110, 433
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 1 cut(s) 324
BstDSI CCRYGG 1 cut(s) 272
BstF5I GGATG 1 cut(s) 297
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 1 cut(s) 127
BstMAI GTCTC 1 cut(s) 320
BstMBI GATC 1 cut(s) 124
BstMWI GCNNNNNNNGC 1 cut(s) 86
BstNI CCWGG 2 cut(s) 166, 456
BstSCI CCNGG 3 cut(s) 164, 235, 454
BstSLI GKGCMC 1 cut(s) 320
BstV1I GCAGC 1 cut(s) 437
BstV2I GAAGAC 1 cut(s) 191
BsuRI GGCC 2 cut(s) 405, 460
BtgI CCRYGG 1 cut(s) 272
BtsCI GGATG 1 cut(s) 297
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 1 cut(s) 318
Cac8I GCNNGC 1 cut(s) 309
CfoI GCGC 1 cut(s) 58
Cfr13I GGNCC 1 cut(s) 458
Csp6I GTAC 2 cut(s) 205, 383
CviAII CATG 2 cut(s) 10, 273
CviJI RGCY 9 cut(s) 80, 89, 169, 216, 323, 405, 428, 438, 460
CviKI_1 RGCY 9 cut(s) 80, 89, 169, 216, 323, 405, 428, 438, 460
CviQI GTAC 2 cut(s) 205, 383
DdeI CTNAG 1 cut(s) 324
DpnI GATC 1 cut(s) 126
DpnII GATC 1 cut(s) 124
DriI GACNNNNNGTC 1 cut(s) 250
Eam1105I GACNNNNNGTC 1 cut(s) 250
Eco130I CCWWGG 2 cut(s) 28, 272
Eco57I CTGAAG 1 cut(s) 185
EcoRII CCWGG 2 cut(s) 164, 454
EcoT14I CCWWGG 2 cut(s) 28, 272
ErhI CCWWGG 2 cut(s) 28, 272
FaeI CATG 2 cut(s) 13, 276
FaiI YATR 7 cut(s) 11, 16, 92, 176, 228, 269, 274
FatI CATG 2 cut(s) 9, 272
FbaI TGATCA 1 cut(s) 124
Fnu4HI GCNGC 1 cut(s) 426
FokI GGATG 1 cut(s) 304
Fsp4HI GCNGC 1 cut(s) 426
FspBI CTAG 2 cut(s) 77, 308
GlaI GCGC 1 cut(s) 57
GluI GCNGC 1 cut(s) 426
GsuI CTGGAG 1 cut(s) 359
HaeIII GGCC 2 cut(s) 405, 460
HapII CCGG 2 cut(s) 135, 236
HhaI GCGC 1 cut(s) 58
Hin1II CATG 2 cut(s) 13, 276
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HinfI GANTC 1 cut(s) 131
HpaII CCGG 2 cut(s) 135, 236
HphI GGTGA 2 cut(s) 172, 433
Hpy166II GTNNAC 2 cut(s) 318, 394
Hpy188III TCNNGA 3 cut(s) 128, 135, 289
Hpy8I GTNNAC 2 cut(s) 318, 394
Hpy99I CGWCG 1 cut(s) 256
HpyCH4III ACNGT 3 cut(s) 25, 110, 433
HpyCH4V TGCA 3 cut(s) 70, 318, 425
HpyF10VI GCNNNNNNNGC 1 cut(s) 86
HpyF3I CTNAG 1 cut(s) 324
Hsp92II CATG 2 cut(s) 13, 276
HspAI GCGC 1 cut(s) 56
Kpn2I TCCGGA 1 cut(s) 134
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 1 cut(s) 124
LpnPI CCDG 9 cut(s) 68, 74, 148, 151, 178, 249, 315, 323, 441
Lsp1109I GCAGC 1 cut(s) 437
LweI GCATC 1 cut(s) 79
MaeI CTAG 2 cut(s) 77, 308
MalI GATC 1 cut(s) 126
MboI GATC 1 cut(s) 124
MboII GAAGA 2 cut(s) 65, 191
MhlI GDGCHC 1 cut(s) 320
MluCI AATT 2 cut(s) 93, 262
MnlI CCTC 5 cut(s) 249, 336, 449, 458, 461
MroI TCCGGA 1 cut(s) 134
MspI CCGG 2 cut(s) 135, 236
MspR9I CCNGG 3 cut(s) 166, 237, 456
MvaI CCWGG 2 cut(s) 166, 456
MwoI GCNNNNNNNGC 1 cut(s) 86
NciI CCSGG 1 cut(s) 237
NcoI CCATGG 1 cut(s) 272
NdeII GATC 1 cut(s) 124
NheI GCTAGC 1 cut(s) 307
NlaIII CATG 2 cut(s) 13, 276
NlaIV GGNNCC 1 cut(s) 347
NmeAIII GCCGAG 1 cut(s) 150
PasI CCCWGGG 1 cut(s) 455
PfeI GAWTC 1 cut(s) 131
PkrI GCNGC 1 cut(s) 427
Psp6I CCWGG 2 cut(s) 164, 454
PspGI CCWGG 2 cut(s) 164, 454
PspN4I GGNNCC 1 cut(s) 347
PspPI GGNCC 1 cut(s) 458
RsaI GTAC 2 cut(s) 206, 384
RsaNI GTAC 2 cut(s) 205, 383
SatI GCNGC 1 cut(s) 426
Sau3AI GATC 1 cut(s) 124
Sau96I GGNCC 1 cut(s) 458
ScaI AGTACT 2 cut(s) 206, 384
ScrFI CCNGG 3 cut(s) 166, 237, 456
SduI GDGCHC 1 cut(s) 320
SetI ASST 4 cut(s) 63, 82, 158, 347
SfaNI GCATC 1 cut(s) 79
Sse9I AATT 2 cut(s) 93, 262
SsiI CCGC 1 cut(s) 444
SspMI CTAG 2 cut(s) 77, 308
StyD4I CCNGG 3 cut(s) 164, 235, 454
StyI CCWWGG 2 cut(s) 28, 272
TaaI ACNGT 3 cut(s) 25, 110, 433
TaqI TCGA 1 cut(s) 254
TasI AATT 2 cut(s) 93, 262
TatI WGTACW 2 cut(s) 204, 382
TfiI GAWTC 1 cut(s) 131
TscAI CASTG 1 cut(s) 318
TseI GCWGC 1 cut(s) 425
TspDTI ATGAA 3 cut(s) 386, 388, 390
TspRI CASTG 1 cut(s) 318
VneI GTGCAC 1 cut(s) 316
XapI RAATTY 1 cut(s) 262
XspI CTAG 2 cut(s) 77, 308
ZrmI AGTACT 2 cut(s) 206, 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.