Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0013784

Physical Location & Seq

Genomic Coordinates Forward (+)

31989829 .. 31992269

Gene Structure 2441 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g33970.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1647 bp

ATGGATAGCCTTGTGATCTTAGCCATTCTGACGATCTTTACTTCAAGTTTTCTTGTAGTACCCATTGTTTCCATAACAGCATCATGCAGCCAAACCCCATACCCTCAAGTTTGCAGCCATTACATTACCAACAACCTCTTATCGACTCTTGATGTTCGTGATCAACGGTTTGCATTTCGTGATCTAGCTCTCAAAGTCACCATGGATCAAGCCGTCAAAGCGCATCGACTTGTTTCATCAGCAGTGAACTCGAAGTCATTGTACAACGAGAGAGCCAAGTTGGCGTGGAATGATTGCTTGGAACTCTATGAGGATACCATTGACAAGCTCAATCGTTCGATGAGTTCCAACAATGCTTTAGACGCTATTACATGGCTAAGTGCATCCATTGCCAATCAACAAACTTGCCAAAACGGTTTCAACGATCTAAACATTCCTAATTCTCATTTACAATCCTCGTTCCCAAACTTCTACAAGCTAAGCACAGACTTGCCTATGTTGCTCAGCAATTCACTTGCGATACACAAGGAGTCTAGTACTTCTTCTTCTTCTTCTTCTTCTACTTCTTCCTCGGCTATGCATTTCAGCAAGCAGGTACGGGGCAGACGCTTATTGCTCAAGTCTGATGCCATATTCCCGGCATGGGTTTCAGCTTCAGATCGAAAGCTTCTACAGTCGAGCGCTGGACCAAAGTCAGCTGATATAGTAGTGGCTCAAGATGGTTCTGGAAATTACAAGACTATAACTGAAGCTGTGGCTGCAGCTAAGAAAAGTAGTGGTAGTGGGACAAAAAGGGTTGTCATATACGTGAAGGCAGGGGTTTACAAAGAGAATGTTGAGGTTAAGAAGACAATGAAGAAGCTGATGTTTGTCGGAGATGGGATGGATGCAACCATTGTTACCGGGAATAAAAATGCCCAAGATGGCTCTACCACTTTTCGCTCTGCCACTTTCGGTGTTTCCGGCGAGGGCTTCATAGCTCAAGACATGACGTTCGAAAACACGGCCGGACCGCAGAAACACCAAGCCGTCGCACTCCGCTCCGGCGCAGACCACTCGGTCTTCTACCGCTGCAGCTTCAAAGGCTACCAAGACACCTTGTACGTCTACTCCCAACGACAGTTCTACCGTGACTGCAACATATACGGCACCGTAGACTTCATCTTCGGCGACGCCTCCGCCATCCTTCAAAACTGTAACATCTACGTCAGAAAGCCATCAAGCAACCAGATCAACACCGTGACGGCACAATCCCGGAGAGACCCTAATGAGAACACCGGCATTATTATCCACAACTGCCGCATAACGGCAGCGCCGGACCTGAGAGCCGTTCAGGGCTCGTTCAGGACCTACTTGGGTCGTCCATGGCAGAAGTACTCAAGGGTGGTGATAATGAAGAGTAACCTAGACGGGTTGATTGAGCCACAAGGTTGGTTTCCTTGGAGTGGAAGTTTTGGTTTGGATACACTCTACTATGGGGAGTACATGAACACTGGAGCTGGTGCTGCCACCGGAGGTAGGGTTAAGTGGCCTGGTTTTCGGGTTATCACGAGTGCCACCGAGGCAGGGAAGTTTACGGTTGGGAATTTCTTGGCCGGAGACGCTTGGCTTCCGGGGACCGGAGTGCCGTTTGAAGCTGGTCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

549

Amino Acids

59.91

Weight (kDa)

9.16

Isoelectric Point (pI)

37.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	24 - 174	7e-30	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	234 - 534	1.4e-145	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013784)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23200
fragaria_vesca	FvH4_4g33970
malus_domestica	MD16G1022000.v1.1 MD16G1022100.v1.1 MD16G1022200.v1.1
prunus_persica	Prupe.1G330800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0443501
rosa_laevigata	RLG00000005923
rosa_multiflora	Rmu_sc0001118.1_g000030
rosa_roxburghii	Rroxscaffold_5G00384040
rosa_rugosa	Rorug04G0346700
rosa_samantha	Rh4AG402400 Rh4BG413900 Rh4CG429600 Rh4DG409600 Rh4DG410900
rosa_wichuraiana	Rw4G034690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1058
Acc36I	ACCTGC	1 cut(s)	583
AccB1I	GGYRCC	1 cut(s)	1148
AccBSI	CCGCTC	1 cut(s)	1041
AccI	GTMKAC	2 cut(s)	1107, 1155
AciI	CCGC	5 cut(s)	1013, 1039, 1069, 1179, 1300
AclWI	GGATC	1 cut(s)	213
AcoI	YGGCCR	2 cut(s)	1005, 1593
AcsI	RAATTY	1 cut(s)	1585
AcuI	CTGAAG	2 cut(s)	639, 768
AcyI	GRCGYC	1 cut(s)	1173
AfaI	GTAC	7 cut(s)	60, 263, 538, 597, 1103, 1376, 1484
AfeI	AGCGCT	1 cut(s)	682
AfiI	CCNNNNNNNGG	6 cut(s)	643, 1306, 1445, 1518, 1566, 1619
AgsI	TTSAA	5 cut(s)	45, 421, 1081, 1190, 1634
AjnI	CCWGG	1 cut(s)	1531
AjuI	GAANNNNNNNTTGG	2 cut(s)	281, 313
Alw26I	GTCTC	2 cut(s)	1254, 1593
AlwI	GGATC	1 cut(s)	213
Aor51HI	AGCGCT	1 cut(s)	682
AoxI	GGCC	3 cut(s)	1005, 1529, 1593
ApeKI	GCWGC	8 cut(s)	87, 114, 758, 761, 1071, 1074, 1310, 1505
ApoI	RAATTY	1 cut(s)	1585
ArsI	GACNNNNNNTTYG	4 cut(s)	977, 1009, 1148, 1180
AspLEI	GCGC	4 cut(s)	223, 683, 1049, 1315
AspS9I	GGNCC	5 cut(s)	686, 1010, 1318, 1347, 1617
AsuC2I	CCSGG	4 cut(s)	638, 904, 1255, 1614
AsuHPI	GGTGA	2 cut(s)	190, 1399
AsuII	TTCGAA	1 cut(s)	996
AvaII	GGWCC	5 cut(s)	686, 1010, 1318, 1347, 1617
BanI	GGYRCC	1 cut(s)	1148
BanII	GRGCYC	1 cut(s)	1340
BarI	GAAGNNNNNNTAC	4 cut(s)	245, 277, 529, 561
BauI	CACGAG	1 cut(s)	1549
BbsI	GAAGAC	2 cut(s)	854, 1054
BbvI	GCAGC	8 cut(s)	99, 126, 745, 773, 1058, 1086, 1322, 1492
BccI	CCATC	6 cut(s)	713, 872, 877, 917, 1190, 1225
BceAI	ACGGC	8 cut(s)	197, 1013, 1020, 1162, 1260, 1313, 1323, 1612
BcgI	CGANNNNNNTGC	4 cut(s)	231, 265, 1038, 1072
BciT130I	CCWGG	1 cut(s)	1533
BciVI	GTATCC	2 cut(s)	307, 1456
BclI	TGATCA	1 cut(s)	160
BcnI	CCSGG	4 cut(s)	638, 904, 1255, 1614
BcoDI	GTCTC	2 cut(s)	1254, 1593
BfaI	CTAG	3 cut(s)	185, 534, 1406
BfmI	CTRYAG	3 cut(s)	671, 759, 1072
BfoI	RGCGCY	2 cut(s)	684, 1316
BfuAI	ACCTGC	1 cut(s)	583
BfuI	GTATCC	2 cut(s)	307, 1456
BglI	GCCNNNNNGGC	2 cut(s)	281, 1562
BisI	GCNGC	9 cut(s)	88, 115, 759, 762, 1072, 1075, 1300, 1311, 1506
BlpI	GCTNAGC	2 cut(s)	479, 503
BlsI	GCNGC	9 cut(s)	89, 116, 760, 763, 1073, 1076, 1301, 1312, 1507
BmcAI	AGTACT	2 cut(s)	538, 1376
Bme1390I	CCNGG	5 cut(s)	638, 904, 1255, 1533, 1614
Bme18I	GGWCC	5 cut(s)	686, 1010, 1318, 1347, 1617
BmgT120I	GGNCC	5 cut(s)	686, 1010, 1318, 1347, 1617
BmiI	GGNNCC	2 cut(s)	1150, 1618
BmrFI	CCNGG	5 cut(s)	638, 904, 1255, 1533, 1614
BmsI	GCATC	5 cut(s)	89, 232, 392, 616, 877
BoxI	GACNNNNGTC	1 cut(s)	691
BpiI	GAAGAC	2 cut(s)	854, 1054
BpmI	CTGGAG	1 cut(s)	1515
Bpu1102I	GCTNAGC	2 cut(s)	479, 503
Bpu14I	TTCGAA	1 cut(s)	996
BpuEI	CTTGAG	5 cut(s)	90, 602, 699, 966, 1363
BpuMI	CCSGG	4 cut(s)	638, 904, 1255, 1614
BsaAI	YACGTR	1 cut(s)	808
BsaHI	GRCGYC	1 cut(s)	1173
BsaI	GGTCTC	1 cut(s)	1254
BsaJI	CCNNGG	6 cut(s)	201, 570, 1364, 1439, 1560, 1613
BsaWI	WCCGGW	2 cut(s)	1511, 1619
BsaXI	ACNNNNNCTCC	6 cut(s)	1094, 1124, 1435, 1465, 1506, 1536
Bsc4I	CCNNNNNNNGG	6 cut(s)	643, 1306, 1445, 1518, 1566, 1619
Bse118I	RCCGGY	1 cut(s)	1277
Bse1I	ACTGG	1 cut(s)	1498
Bse3DI	GCAATG	1 cut(s)	387
BseBI	CCWGG	1 cut(s)	1533
BseDI	CCNNGG	6 cut(s)	201, 570, 1364, 1439, 1560, 1613
BseGI	GGATG	4 cut(s)	383, 888, 892, 1182
BseLI	CCNNNNNNNGG	6 cut(s)	643, 1306, 1445, 1518, 1566, 1619
BseMI	GCAATG	1 cut(s)	387
BseMII	CTCAG	2 cut(s)	517, 1313
BseNI	ACTGG	1 cut(s)	1498
BseX3I	CGGCCG	1 cut(s)	1005
BseXI	GCAGC	8 cut(s)	99, 126, 745, 773, 1058, 1086, 1322, 1492
Bsh1285I	CGRYCG	1 cut(s)	1008
BshFI	GGCC	3 cut(s)	1007, 1531, 1595
BshNI	GGYRCC	1 cut(s)	1148
BsiEI	CGRYCG	1 cut(s)	1008
BslFI	GGGAC	2 cut(s)	799, 1630
BslI	CCNNNNNNNGG	6 cut(s)	643, 1306, 1445, 1518, 1566, 1619
BsmAI	GTCTC	2 cut(s)	1254, 1593
BsmBI	CGTCTC	1 cut(s)	1593
BsmFI	GGGAC	2 cut(s)	799, 1630
BsnI	GGCC	3 cut(s)	1007, 1531, 1595
Bso31I	GGTCTC	1 cut(s)	1254
Bsp119I	TTCGAA	1 cut(s)	996
Bsp1286I	GDGCHC	1 cut(s)	1340
Bsp1407I	TGTACA	1 cut(s)	261
Bsp143I	GATC	8 cut(s)	15, 33, 160, 181, 205, 424, 658, 1230
Bsp1720I	GCTNAGC	2 cut(s)	479, 503
Bsp19I	CCATGG	2 cut(s)	201, 1364
BspACI	CCGC	5 cut(s)	1013, 1039, 1069, 1179, 1300
BspANI	GGCC	3 cut(s)	1007, 1531, 1595
BspCNI	CTCAG	2 cut(s)	516, 1314
BspLI	GGNNCC	2 cut(s)	1150, 1618
BspMAI	CTGCAG	2 cut(s)	763, 1076
BspMI	ACCTGC	1 cut(s)	583
BspPI	GGATC	1 cut(s)	213
BspT104I	TTCGAA	1 cut(s)	996
BspT107I	GGYRCC	1 cut(s)	1148
BspTNI	GGTCTC	1 cut(s)	1254
BsrBI	CCGCTC	1 cut(s)	1041
BsrDI	GCAATG	1 cut(s)	387
BsrFI	RCCGGY	1 cut(s)	1277
BsrGI	TGTACA	1 cut(s)	261
BsrI	ACTGG	1 cut(s)	1498
BssAI	RCCGGY	1 cut(s)	1277
BssECI	CCNNGG	6 cut(s)	201, 570, 1364, 1439, 1560, 1613
BssMI	GATC	8 cut(s)	15, 33, 160, 181, 205, 424, 658, 1230
BssNI	GRCGYC	1 cut(s)	1173
BssSI	CACGAG	1 cut(s)	1549
BssT1I	CCWWGG	3 cut(s)	201, 1364, 1439
Bst2BI	CACGAG	1 cut(s)	1549
Bst2UI	CCWGG	1 cut(s)	1533
Bst4CI	ACNGT	9 cut(s)	168, 416, 675, 1122, 1130, 1153, 1196, 1240, 1579
Bst6I	CTCTTC	1 cut(s)	1391
BstACI	GRCGYC	1 cut(s)	1173
BstAPI	GCANNNNNTGC	1 cut(s)	389
BstAUI	TGTACA	1 cut(s)	261
BstBAI	YACGTR	1 cut(s)	808
BstBI	TTCGAA	1 cut(s)	996
BstC8I	GCNNGC	1 cut(s)	590
BstDEI	CTNAG	6 cut(s)	19, 377, 479, 503, 765, 1322
BstDSI	CCRYGG	2 cut(s)	201, 1364
BstF5I	GGATG	4 cut(s)	383, 888, 892, 1182
BstH2I	RGCGCY	2 cut(s)	684, 1316
BstHHI	GCGC	4 cut(s)	223, 683, 1049, 1315
BstKTI	GATC	8 cut(s)	18, 36, 163, 184, 208, 427, 661, 1233
BstMAI	GTCTC	2 cut(s)	1254, 1593
BstMBI	GATC	8 cut(s)	15, 33, 160, 181, 205, 424, 658, 1230
BstMCI	CGRYCG	1 cut(s)	1008
BstNI	CCWGG	1 cut(s)	1533
BstPAI	GACNNNNGTC	1 cut(s)	691
BstSCI	CCNGG	5 cut(s)	636, 902, 1253, 1531, 1612
BstSFI	CTRYAG	3 cut(s)	671, 759, 1072
BstV1I	GCAGC	8 cut(s)	99, 126, 745, 773, 1058, 1086, 1322, 1492
BstV2I	GAAGAC	2 cut(s)	854, 1054
BstXI	CCANNNNNNTGG	1 cut(s)	1431
BstZI	CGGCCG	1 cut(s)	1005
BsuI	GTATCC	2 cut(s)	307, 1456
BsuRI	GGCC	3 cut(s)	1007, 1531, 1595
BtgI	CCRYGG	2 cut(s)	201, 1364
BtsCI	GGATG	4 cut(s)	383, 888, 892, 1182
BtsI	GCAGTG	1 cut(s)	249
BtsIMutI	CAGTG	2 cut(s)	249, 1491
BveI	ACCTGC	1 cut(s)	583
Cac8I	GCNNGC	1 cut(s)	590
CfoI	GCGC	4 cut(s)	223, 683, 1049, 1315
Cfr10I	RCCGGY	1 cut(s)	1277
Cfr13I	GGNCC	5 cut(s)	686, 1010, 1318, 1347, 1617
CpoI	CGGWCCG	1 cut(s)	1010
CseI	GACGC	4 cut(s)	371, 615, 1181, 1610
Csp6I	GTAC	7 cut(s)	59, 262, 537, 596, 1102, 1375, 1483
CspI	CGGWCCG	1 cut(s)	1010
CviAII	CATG	7 cut(s)	84, 202, 372, 642, 988, 1365, 1486
CviQI	GTAC	7 cut(s)	59, 262, 537, 596, 1102, 1375, 1483
DdeI	CTNAG	6 cut(s)	19, 377, 479, 503, 765, 1322
DpnI	GATC	8 cut(s)	17, 35, 162, 183, 207, 426, 660, 1232
DpnII	GATC	8 cut(s)	15, 33, 160, 181, 205, 424, 658, 1230
DrdI	GACNNNNNNGTC	1 cut(s)	1058
DseDI	GACNNNNNNGTC	1 cut(s)	1058
EaeI	YGGCCR	2 cut(s)	1005, 1593
EagI	CGGCCG	1 cut(s)	1005
Eam1104I	CTCTTC	1 cut(s)	1391
EarI	CTCTTC	1 cut(s)	1391
EciI	GGCGGA	1 cut(s)	1168
EclXI	CGGCCG	1 cut(s)	1005
Eco130I	CCWWGG	3 cut(s)	201, 1364, 1439
Eco24I	GRGCYC	1 cut(s)	1340
Eco31I	GGTCTC	1 cut(s)	1254
Eco47I	GGWCC	5 cut(s)	686, 1010, 1318, 1347, 1617
Eco47III	AGCGCT	1 cut(s)	682
Eco52I	CGGCCG	1 cut(s)	1005
Eco57I	CTGAAG	2 cut(s)	639, 768
EcoO109I	RGGNCCY	1 cut(s)	1347
EcoRII	CCWGG	1 cut(s)	1531
EcoT14I	CCWWGG	3 cut(s)	201, 1364, 1439
EcoT22I	ATGCAT	1 cut(s)	582
EcoT38I	GRGCYC	1 cut(s)	1340
ErhI	CCWWGG	3 cut(s)	201, 1364, 1439
Esp3I	CGTCTC	1 cut(s)	1593
FaeI	CATG	7 cut(s)	87, 205, 375, 645, 991, 1368, 1489
FaqI	GGGAC	2 cut(s)	799, 1630
FatI	CATG	7 cut(s)	83, 201, 371, 641, 987, 1364, 1485
FbaI	TGATCA	1 cut(s)	160
FblI	GTMKAC	2 cut(s)	1107, 1155
Fnu4HI	GCNGC	9 cut(s)	88, 115, 759, 762, 1072, 1075, 1300, 1311, 1506
FokI	GGATG	4 cut(s)	370, 895, 899, 1169
FriOI	GRGCYC	1 cut(s)	1340
Fsp4HI	GCNGC	9 cut(s)	88, 115, 759, 762, 1072, 1075, 1300, 1311, 1506
FspBI	CTAG	3 cut(s)	185, 534, 1406
GlaI	GCGC	4 cut(s)	222, 682, 1048, 1314
GluI	GCNGC	9 cut(s)	88, 115, 759, 762, 1072, 1075, 1300, 1311, 1506
GsuI	CTGGAG	1 cut(s)	1515
HaeII	RGCGCY	2 cut(s)	684, 1316
HaeIII	GGCC	3 cut(s)	1007, 1531, 1595
HgaI	GACGC	4 cut(s)	371, 615, 1181, 1610
HhaI	GCGC	4 cut(s)	223, 683, 1049, 1315
Hin1I	GRCGYC	1 cut(s)	1173
Hin1II	CATG	7 cut(s)	87, 205, 375, 645, 991, 1368, 1489
Hin6I	GCGC	4 cut(s)	221, 681, 1047, 1313
HinP1I	GCGC	4 cut(s)	221, 681, 1047, 1313
HindIII	AAGCTT	1 cut(s)	665
HinfI	GANTC	2 cut(s)	145, 530
HphI	GGTGA	2 cut(s)	190, 1399
Hpy166II	GTNNAC	5 cut(s)	247, 823, 1108, 1156, 1575
Hpy188I	TCNGA	5 cut(s)	30, 625, 658, 875, 1211
Hpy188III	TCNNGA	8 cut(s)	149, 158, 179, 716, 726, 983, 1345, 1549
Hpy8I	GTNNAC	5 cut(s)	247, 823, 1108, 1156, 1575
Hpy99I	CGWCG	2 cut(s)	1034, 1175
HpyAV	CCTTC	2 cut(s)	805, 1196
HpyCH4III	ACNGT	9 cut(s)	168, 416, 675, 1122, 1130, 1153, 1196, 1240, 1579
HpyCH4IV	ACGT	4 cut(s)	807, 992, 1104, 1206
HpyCH4V	TGCA	9 cut(s)	87, 114, 173, 383, 580, 761, 890, 1074, 1137
HpyF3I	CTNAG	6 cut(s)	19, 377, 479, 503, 765, 1322
HpySE526I	ACGT	4 cut(s)	807, 992, 1104, 1206
Hsp92I	GRCGYC	1 cut(s)	1173
Hsp92II	CATG	7 cut(s)	87, 205, 375, 645, 991, 1368, 1489
HspAI	GCGC	4 cut(s)	221, 681, 1047, 1313
Ksp22I	TGATCA	1 cut(s)	160
Kzo9I	GATC	8 cut(s)	15, 33, 160, 181, 205, 424, 658, 1230
LmnI	GCTCC	2 cut(s)	1046, 1496
Lsp1109I	GCAGC	8 cut(s)	99, 126, 745, 773, 1058, 1086, 1322, 1492
LweI	GCATC	5 cut(s)	89, 232, 392, 616, 877
MaeI	CTAG	3 cut(s)	185, 534, 1406
MaeII	ACGT	4 cut(s)	807, 992, 1104, 1206
MaeIII	GTNAC	6 cut(s)	196, 898, 1130, 1196, 1240, 1400
MalI	GATC	8 cut(s)	17, 35, 162, 183, 207, 426, 660, 1232
MbiI	CCGCTC	1 cut(s)	1041
MboI	GATC	8 cut(s)	15, 33, 160, 181, 205, 424, 658, 1230
MhlI	GDGCHC	1 cut(s)	1340
MluCI	AATT	4 cut(s)	439, 508, 730, 1585
MlyI	GAGTC	2 cut(s)	139, 539
MmeI	TCCRAC	2 cut(s)	372, 853
Mph1103I	ATGCAT	1 cut(s)	582
MseI	TTAA	2 cut(s)	843, 1524
MslI	CAYNNNNRTG	1 cut(s)	806
MspA1I	CMGCKG	2 cut(s)	698, 1071
MspR9I	CCNGG	5 cut(s)	638, 904, 1255, 1533, 1614
MvaI	CCWGG	1 cut(s)	1533
NciI	CCSGG	4 cut(s)	638, 904, 1255, 1614
NcoI	CCATGG	2 cut(s)	201, 1364
NdeII	GATC	8 cut(s)	15, 33, 160, 181, 205, 424, 658, 1230
NlaIII	CATG	7 cut(s)	87, 205, 375, 645, 991, 1368, 1489
NlaIV	GGNNCC	2 cut(s)	1150, 1618
NmeAIII	GCCGAG	1 cut(s)	551
NmuCI	GTSAC	3 cut(s)	196, 1130, 1240
NsiI	ATGCAT	1 cut(s)	582
NspV	TTCGAA	1 cut(s)	996
PcsI	WCGNNNNNNNCGW	1 cut(s)	420
PfoI	TCCNGGA	1 cut(s)	1253
PkrI	GCNGC	9 cut(s)	89, 116, 760, 763, 1073, 1076, 1301, 1312, 1507
PleI	GAGTC	2 cut(s)	139, 538
PpsI	GAGTC	2 cut(s)	139, 538
Ppu21I	YACGTR	1 cut(s)	808
PpuMI	RGGWCCY	1 cut(s)	1347
PshAI	GACNNNNGTC	1 cut(s)	691
Psp5II	RGGWCCY	1 cut(s)	1347
Psp6I	CCWGG	1 cut(s)	1531
PspGI	CCWGG	1 cut(s)	1531
PspN4I	GGNNCC	2 cut(s)	1150, 1618
PspPI	GGNCC	5 cut(s)	686, 1010, 1318, 1347, 1617
PspPPI	RGGWCCY	1 cut(s)	1347
PstI	CTGCAG	2 cut(s)	763, 1076
PvuII	CAGCTG	1 cut(s)	698
RsaI	GTAC	7 cut(s)	60, 263, 538, 597, 1103, 1376, 1484
RsaNI	GTAC	7 cut(s)	59, 262, 537, 596, 1102, 1375, 1483
RseI	CAYNNNNRTG	1 cut(s)	806
Rsr2I	CGGWCCG	1 cut(s)	1010
RsrII	CGGWCCG	1 cut(s)	1010
SaqAI	TTAA	2 cut(s)	843, 1524
SatI	GCNGC	9 cut(s)	88, 115, 759, 762, 1072, 1075, 1300, 1311, 1506
Sau3AI	GATC	8 cut(s)	15, 33, 160, 181, 205, 424, 658, 1230
Sau96I	GGNCC	5 cut(s)	686, 1010, 1318, 1347, 1617
ScaI	AGTACT	2 cut(s)	538, 1376
SchI	GAGTC	2 cut(s)	139, 539
ScrFI	CCNGG	5 cut(s)	638, 904, 1255, 1533, 1614
SduI	GDGCHC	1 cut(s)	1340
SfaNI	GCATC	5 cut(s)	89, 232, 392, 616, 877
SfcI	CTRYAG	3 cut(s)	671, 759, 1072
SfuI	TTCGAA	1 cut(s)	996
SinI	GGWCC	5 cut(s)	686, 1010, 1318, 1347, 1617
SmiMI	CAYNNNNRTG	1 cut(s)	806
SmlI	CTYRAG	5 cut(s)	105, 617, 714, 981, 1378
SmoI	CTYRAG	5 cut(s)	105, 617, 714, 981, 1378
Sse9I	AATT	4 cut(s)	439, 508, 730, 1585
SsiI	CCGC	5 cut(s)	1013, 1039, 1069, 1179, 1300
SspMI	CTAG	3 cut(s)	185, 534, 1406
StyD4I	CCNGG	5 cut(s)	636, 902, 1253, 1531, 1612
StyI	CCWWGG	3 cut(s)	201, 1364, 1439
TaaI	ACNGT	9 cut(s)	168, 416, 675, 1122, 1130, 1153, 1196, 1240, 1579
TaiI	ACGT	4 cut(s)	810, 995, 1107, 1209
TaqI	TCGA	7 cut(s)	143, 226, 251, 338, 661, 677, 996
TaqII	GACCGA	1 cut(s)	1048
TasI	AATT	4 cut(s)	439, 508, 730, 1585
TatI	WGTACW	4 cut(s)	261, 536, 1374, 1482
TauI	GCSGC	1 cut(s)	1302
Tru1I	TTAA	2 cut(s)	843, 1524
Tru9I	TTAA	2 cut(s)	843, 1524
TscAI	CASTG	2 cut(s)	249, 1498
TseFI	GTSAC	3 cut(s)	196, 1130, 1240
TseI	GCWGC	8 cut(s)	87, 114, 758, 761, 1071, 1074, 1310, 1505
Tsp45I	GTSAC	3 cut(s)	196, 1130, 1240
TspDTI	ATGAA	6 cut(s)	225, 869, 964, 1150, 1409, 1502
TspRI	CASTG	2 cut(s)	249, 1498
VpaK11BI	GGWCC	5 cut(s)	686, 1010, 1318, 1347, 1617
XapI	RAATTY	1 cut(s)	1585
XmiI	GTMKAC	2 cut(s)	1107, 1155
XspI	CTAG	3 cut(s)	185, 534, 1406
ZrmI	AGTACT	2 cut(s)	538, 1376
Zsp2I	ATGCAT	1 cut(s)	582

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.