Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0013784

Physical Location & Seq

Genomic Coordinates Forward (+)

1609218 .. 1612142

Gene Structure 2925 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1022200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1596 bp

ATGGAGTTCAATGCATATTTTCTTGTGTTCTTTTCTCTTCTATTCGTGCTGCAGTATAGTTCTTGTAGAATCACTGCACCATGCAGTCAAACCCCATACCCTCAAGTGTGTCGCCATTACATTAACACTAACCTCTTATCAACCCTAGATCGTCACCAAAGGTTTGCAGTTCGTGACTTGGCACTTAAAGTGTCCATGGACCAAGCCCTTAAAGCTCATCAACTTGTCTCGTCCTTGAACTTGAACTCATTGGACGGTGACCATACTCGAGCCAAGGGGGCCTGGAATGACTGCTTGGAGCTCTACGAGGATACCGTTGACAAGCTCAACCGTTCCATCGGCTCCACCAATGCCCTTGATGTCAATACATGGTTGAGTGCATCGCTTACTAATCAACAGACATGCCAAAATGGTTTCAAAAACCTCAACATTCCTAGTTCTCATTTGCAATCTTTCCCAAACTACGTCACGCTCTCAACCAACTTGTCCAAGATGCTTAGCAACTCACTATCAATACACAAGGTCTCAACTTCTTCATCAGCTCTACATTTTAGCAAACAAGTTCGTGGAAGACGACGGCGTTTGCTTTCGGATGATGGGTTCCCAGAATGGGTTTCAGCCGCTGATCGAAAGCTGCTACAATCGATAGGTAGCGGACCAAACGCTGATATGGTAGTAGCACAAGACGGGTCCGGGAATTACAAGTCAATTTCTGAAGCTGTGGATGCTGCGTACAAGCTACAGGGTGGGGCGACTAAAAGGTTTGTCATACACGTGAAGGCAGGAGTTTACAGAGAAAATATCGAGATTAAGAGGACAATGAAGAACATCATGTTCATTGGAGATGGTATCGATGCAACGATTGTCACAGGGAATAAAAATGCCCAAGATGGTTCAACTACATATCGCTCTGCCACATTTGGGGCTACCGGAGATGGCTTTATTGCTCAAGACATGACATTCGAAAACACTGCCGGACCACAGAAACACCAAGCCGTGGCACTCCGCTCCGGCTCCGATCATTCAGTCTTCTACCGTTGCAGCTTCAAGGGTTACCAAGACACCTTATACGTGTATTCCCAACGCCAATTCTACCGCGACTGCAACATATACGGCACGGTCGACTTCATCTGCGGCGACGCTACGGTTGTGCTCCAAAATTGCAACATCTACGCGAGGAAACCAATGAGCAACCAAGTAAACACCGTGACAGCGCAGTCTAGGACCGACCCGAATGAAAACACAGGCATTGTTATTCATAATTCGCGAATCACGGCTGCAGAACAAGGTTCGTTTAAGACGTACTTGGGTAGGCCGTGGAAAGAGTACTCTAGGACGGTAATTATGAAGAGTAATCTTGATGGGATCAATCCTGCTGGTTGGTTTCCGTGGAGTGGAAGCTTTGCCCTCTCCACACTTTATTACGGGGAGTACATGAACTCCGGCGCTGGAGCTGGTACTGGAGGGAGGGTCAACTGGCCCGGTTATCGTGTTATAACGAGTGCAGTGGAGGCCGGAAAGTTTACGGTTGGGAACTTTTTGGCCGGAGATTCGTGGATTCCGGGGACCAGAGTTCCATTTACAGCTGGTCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

532

Amino Acids

58.61

Weight (kDa)

9.09

Isoelectric Point (pI)

33.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	25 - 172	1.2e-31	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	223 - 517	8.3e-139	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013784)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23200
fragaria_vesca	FvH4_4g33970
malus_domestica	MD16G1022000.v1.1 MD16G1022100.v1.1 MD16G1022200.v1.1
prunus_persica	Prupe.1G330800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0443501
rosa_laevigata	RLG00000005923
rosa_multiflora	Rmu_sc0001118.1_g000030
rosa_roxburghii	Rroxscaffold_5G00384040
rosa_rugosa	Rorug04G0346700
rosa_samantha	Rh4AG402400 Rh4BG413900 Rh4CG429600 Rh4DG409600 Rh4DG410900
rosa_wichuraiana	Rw4G034690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1496
AasI	GACNNNNNNGTC	1 cut(s)	1216
AccB7I	CCANNNNNTGG	1 cut(s)	997
AccBSI	CCGCTC	1 cut(s)	1008
AccI	GTMKAC	1 cut(s)	1122
AccII	CGCG	3 cut(s)	1098, 1175, 1267
AciI	CCGC	5 cut(s)	621, 654, 1006, 1096, 1134
AclWI	GGATC	1 cut(s)	1373
AcoI	YGGCCR	1 cut(s)	1542
AcuI	CTGAAG	1 cut(s)	735
AcvI	CACGTG	1 cut(s)	775
AfaI	GTAC	5 cut(s)	734, 1304, 1328, 1433, 1459
AfiI	CCNNNNNNNGG	4 cut(s)	610, 921, 997, 1394
AflIII	ACRYGT	2 cut(s)	772, 1071
AgsI	TTSAA	6 cut(s)	10, 238, 244, 418, 897, 1048
AjnI	CCWGG	1 cut(s)	281
AjuI	GAANNNNNNNTTGG	2 cut(s)	278, 310
AloI	GAACNNNNNNTCC	2 cut(s)	584, 616
Alw21I	GWGCWC	2 cut(s)	303, 1155
Alw26I	GTCTC	2 cut(s)	232, 529
AlwI	GGATC	1 cut(s)	1373
AlwNI	CAGNNNCTG	1 cut(s)	623
Ama87I	CYCGRG	1 cut(s)	267
AoxI	GGCC	5 cut(s)	279, 1313, 1478, 1512, 1542
ApeKI	GCWGC	5 cut(s)	49, 634, 728, 1041, 1277
ArsI	GACNNNNNNTTYG	2 cut(s)	944, 976
AspLEI	GCGC	2 cut(s)	1216, 1448
AspS9I	GGNCC	8 cut(s)	199, 279, 656, 690, 977, 1224, 1479, 1566
AsuC2I	CCSGG	3 cut(s)	694, 1482, 1563
AsuHPI	GGTGA	2 cut(s)	146, 269
AsuII	TTCGAA	1 cut(s)	963
AvaI	CYCGRG	1 cut(s)	267
AvaII	GGWCC	6 cut(s)	199, 656, 690, 977, 1224, 1566
BanII	GRGCYC	1 cut(s)	303
BbrPI	CACGTG	1 cut(s)	775
BbsI	GAAGAC	2 cut(s)	577, 1021
Bbv12I	GWGCWC	2 cut(s)	303, 1155
BbvI	GCAGC	5 cut(s)	36, 621, 715, 1053, 1264
BccI	CCATC	6 cut(s)	344, 590, 839, 884, 929, 1355
BceAI	ACGGC	5 cut(s)	593, 980, 1129, 1290, 1300
BcgI	CGANNNNNNTGC	2 cut(s)	953, 987
BciT130I	CCWGG	1 cut(s)	283
BciVI	GTATCC	1 cut(s)	304
BcnI	CCSGG	3 cut(s)	694, 1482, 1563
BcoDI	GTCTC	2 cut(s)	232, 529
BfaI	CTAG	4 cut(s)	146, 435, 1221, 1332
BfmI	CTRYAG	3 cut(s)	50, 740, 1278
BfoI	RGCGCY	1 cut(s)	1449
BfuI	GTATCC	1 cut(s)	304
BglI	GCCNNNNNGGC	1 cut(s)	278
BisI	GCNGC	7 cut(s)	50, 621, 635, 729, 1042, 1135, 1278
BlpI	GCTNAGC	1 cut(s)	497
BlsI	GCNGC	7 cut(s)	51, 622, 636, 730, 1043, 1136, 1279
BmcAI	AGTACT	1 cut(s)	1328
Bme1390I	CCNGG	4 cut(s)	283, 694, 1482, 1563
Bme18I	GGWCC	6 cut(s)	199, 656, 690, 977, 1224, 1566
BmeT110I	CYCGRG	1 cut(s)	267
BmgT120I	GGNCC	8 cut(s)	199, 279, 656, 690, 977, 1224, 1479, 1566
BmiI	GGNNCC	6 cut(s)	280, 343, 602, 691, 1015, 1567
BmrFI	CCNGG	4 cut(s)	283, 694, 1482, 1563
BmsI	GCATC	4 cut(s)	389, 483, 715, 844
BpiI	GAAGAC	2 cut(s)	577, 1021
BpmI	CTGGAG	2 cut(s)	1470, 1482
Bpu1102I	GCTNAGC	1 cut(s)	497
Bpu14I	TTCGAA	1 cut(s)	963
BpuEI	CTTGAG	2 cut(s)	87, 933
BpuMI	CCSGG	3 cut(s)	694, 1482, 1563
Bsa29I	ATCGAT	2 cut(s)	644, 852
BsaAI	YACGTR	2 cut(s)	775, 1072
BsaI	GGTCTC	1 cut(s)	529
BsaJI	CCNNGG	6 cut(s)	195, 273, 996, 1316, 1388, 1562
BsaWI	WCCGGW	1 cut(s)	929
BsaXI	ACNNNNNCTCC	4 cut(s)	1424, 1454, 1455, 1485
Bsc4I	CCNNNNNNNGG	4 cut(s)	610, 921, 997, 1394
Bse1I	ACTGG	2 cut(s)	1465, 1481
BseBI	CCWGG	1 cut(s)	283
BseCI	ATCGAT	2 cut(s)	644, 852
BseDI	CCNNGG	6 cut(s)	195, 273, 996, 1316, 1388, 1562
BseGI	GGATG	2 cut(s)	598, 730
BseLI	CCNNNNNNNGG	4 cut(s)	610, 921, 997, 1394
BseNI	ACTGG	2 cut(s)	1465, 1481
BseXI	GCAGC	5 cut(s)	36, 621, 715, 1053, 1264
BsgI	GTGCAG	2 cut(s)	60, 1524
Bsh1236I	CGCG	3 cut(s)	1098, 1175, 1267
Bsh1285I	CGRYCG	1 cut(s)	1122
BshFI	GGCC	5 cut(s)	281, 1315, 1480, 1514, 1544
BshVI	ATCGAT	2 cut(s)	644, 852
BsiEI	CGRYCG	1 cut(s)	1122
BsiHKAI	GWGCWC	2 cut(s)	303, 1155
BsiHKCI	CYCGRG	1 cut(s)	267
BsiSI	CCGG	9 cut(s)	693, 930, 975, 1011, 1443, 1482, 1515, 1545, 1562
BslFI	GGGAC	1 cut(s)	1579
BslI	CCNNNNNNNGG	4 cut(s)	610, 921, 997, 1394
BsmAI	GTCTC	2 cut(s)	232, 529
BsmFI	GGGAC	1 cut(s)	1579
BsnI	GGCC	5 cut(s)	281, 1315, 1480, 1514, 1544
Bso31I	GGTCTC	1 cut(s)	529
BsoBI	CYCGRG	1 cut(s)	267
Bsp119I	TTCGAA	1 cut(s)	963
Bsp1286I	GDGCHC	2 cut(s)	303, 1155
Bsp143I	GATC	4 cut(s)	148, 625, 1018, 1365
Bsp1720I	GCTNAGC	1 cut(s)	497
Bsp19I	CCATGG	1 cut(s)	195
Bsp68I	TCGCGA	1 cut(s)	1267
BspACI	CCGC	5 cut(s)	621, 654, 1006, 1096, 1134
BspANI	GGCC	5 cut(s)	281, 1315, 1480, 1514, 1544
BspDI	ATCGAT	2 cut(s)	644, 852
BspFNI	CGCG	3 cut(s)	1098, 1175, 1267
BspLI	GGNNCC	6 cut(s)	280, 343, 602, 691, 1015, 1567
BspMAI	CTGCAG	2 cut(s)	54, 1282
BspPI	GGATC	1 cut(s)	1373
BspT104I	TTCGAA	1 cut(s)	963
BspTNI	GGTCTC	1 cut(s)	529
BsrBI	CCGCTC	1 cut(s)	1008
BsrI	ACTGG	2 cut(s)	1465, 1481
BssECI	CCNNGG	6 cut(s)	195, 273, 996, 1316, 1388, 1562
BssMI	GATC	4 cut(s)	148, 625, 1018, 1365
BssT1I	CCWWGG	2 cut(s)	195, 273
Bst2UI	CCWGG	1 cut(s)	283
Bst4CI	ACNGT	9 cut(s)	257, 316, 332, 1037, 1120, 1147, 1207, 1339, 1528
Bst6I	CTCTTC	2 cut(s)	42, 1343
BstBAI	YACGTR	2 cut(s)	775, 1072
BstBI	TTCGAA	1 cut(s)	963
BstDEI	CTNAG	1 cut(s)	497
BstDSI	CCRYGG	4 cut(s)	195, 996, 1316, 1388
BstEII	GGTNACC	2 cut(s)	257, 1052
BstF5I	GGATG	2 cut(s)	598, 730
BstFNI	CGCG	3 cut(s)	1098, 1175, 1267
BstH2I	RGCGCY	1 cut(s)	1449
BstHHI	GCGC	2 cut(s)	1216, 1448
BstKTI	GATC	4 cut(s)	151, 628, 1021, 1368
BstMAI	GTCTC	2 cut(s)	232, 529
BstMBI	GATC	4 cut(s)	148, 625, 1018, 1365
BstMCI	CGRYCG	1 cut(s)	1122
BstMWI	GCNNNNNNNGC	4 cut(s)	212, 278, 725, 1511
BstNI	CCWGG	1 cut(s)	283
BstNSI	RCATGY	1 cut(s)	405
BstPI	GGTNACC	2 cut(s)	257, 1052
BstSCI	CCNGG	4 cut(s)	281, 692, 1480, 1561
BstSFI	CTRYAG	3 cut(s)	50, 740, 1278
BstUI	CGCG	3 cut(s)	1098, 1175, 1267
BstV1I	GCAGC	5 cut(s)	36, 621, 715, 1053, 1264
BstV2I	GAAGAC	2 cut(s)	577, 1021
Bsu15I	ATCGAT	2 cut(s)	644, 852
BsuI	GTATCC	1 cut(s)	304
BsuRI	GGCC	5 cut(s)	281, 1315, 1480, 1514, 1544
BsuTUI	ATCGAT	2 cut(s)	644, 852
BtgI	CCRYGG	4 cut(s)	195, 996, 1316, 1388
BtgZI	GCGATG	1 cut(s)	366
BtsCI	GGATG	2 cut(s)	598, 730
BtsI	GCAGTG	3 cut(s)	72, 969, 1512
BtsIMutI	CAGTG	3 cut(s)	72, 969, 1512
BtuMI	TCGCGA	1 cut(s)	1267
CaiI	CAGNNNCTG	1 cut(s)	623
CfoI	GCGC	2 cut(s)	1216, 1448
Cfr13I	GGNCC	8 cut(s)	199, 279, 656, 690, 977, 1224, 1479, 1566
ClaI	ATCGAT	2 cut(s)	644, 852
CseI	GACGC	1 cut(s)	1148
Csp6I	GTAC	5 cut(s)	733, 1303, 1327, 1432, 1458
CviAII	CATG	7 cut(s)	81, 196, 369, 402, 832, 955, 1435
CviQI	GTAC	5 cut(s)	733, 1303, 1327, 1432, 1458
DdeI	CTNAG	1 cut(s)	497
DpnI	GATC	4 cut(s)	150, 627, 1020, 1367
DpnII	GATC	4 cut(s)	148, 625, 1018, 1365
DrdI	GACNNNNNNGTC	1 cut(s)	1216
DseDI	GACNNNNNNGTC	1 cut(s)	1216
EaeI	YGGCCR	1 cut(s)	1542
Eam1104I	CTCTTC	2 cut(s)	42, 1343
EarI	CTCTTC	2 cut(s)	42, 1343
Ecl136II	GAGCTC	1 cut(s)	301
Eco130I	CCWWGG	2 cut(s)	195, 273
Eco24I	GRGCYC	1 cut(s)	303
Eco31I	GGTCTC	1 cut(s)	529
Eco47I	GGWCC	6 cut(s)	199, 656, 690, 977, 1224, 1566
Eco53kI	GAGCTC	1 cut(s)	301
Eco57I	CTGAAG	1 cut(s)	735
Eco72I	CACGTG	1 cut(s)	775
Eco88I	CYCGRG	1 cut(s)	267
Eco91I	GGTNACC	2 cut(s)	257, 1052
EcoICRI	GAGCTC	1 cut(s)	301
EcoO109I	RGGNCCY	1 cut(s)	279
EcoO65I	GGTNACC	2 cut(s)	257, 1052
EcoRII	CCWGG	1 cut(s)	281
EcoT14I	CCWWGG	2 cut(s)	195, 273
EcoT22I	ATGCAT	1 cut(s)	16
EcoT38I	GRGCYC	1 cut(s)	303
ErhI	CCWWGG	2 cut(s)	195, 273
FaeI	CATG	7 cut(s)	84, 199, 372, 405, 835, 958, 1438
FalI	AAGNNNNNCTT	2 cut(s)	1289, 1321
FaqI	GGGAC	1 cut(s)	1579
FatI	CATG	7 cut(s)	80, 195, 368, 401, 831, 954, 1434
FblI	GTMKAC	1 cut(s)	1122
Fnu4HI	GCNGC	7 cut(s)	50, 621, 635, 729, 1042, 1135, 1278
FokI	GGATG	2 cut(s)	605, 737
FriOI	GRGCYC	1 cut(s)	303
Fsp4HI	GCNGC	7 cut(s)	50, 621, 635, 729, 1042, 1135, 1278
FspBI	CTAG	4 cut(s)	146, 435, 1221, 1332
GlaI	GCGC	2 cut(s)	1215, 1447
GluI	GCNGC	7 cut(s)	50, 621, 635, 729, 1042, 1135, 1278
GsuI	CTGGAG	2 cut(s)	1470, 1482
HaeII	RGCGCY	1 cut(s)	1449
HaeIII	GGCC	5 cut(s)	281, 1315, 1480, 1514, 1544
HapII	CCGG	9 cut(s)	693, 930, 975, 1011, 1443, 1482, 1515, 1545, 1562
HgaI	GACGC	1 cut(s)	1148
HhaI	GCGC	2 cut(s)	1216, 1448
Hin1II	CATG	7 cut(s)	84, 199, 372, 405, 835, 958, 1438
Hin6I	GCGC	2 cut(s)	1214, 1446
HinP1I	GCGC	2 cut(s)	1214, 1446
HincII	GTYRAC	3 cut(s)	319, 1123, 1474
HindII	GTYRAC	3 cut(s)	319, 1123, 1474
HindIII	AAGCTT	1 cut(s)	1399
HinfI	GANTC	4 cut(s)	69, 1269, 1550, 1558
HpaII	CCGG	9 cut(s)	693, 930, 975, 1011, 1443, 1482, 1515, 1545, 1562
HphI	GGTGA	2 cut(s)	146, 269
Hpy166II	GTNNAC	6 cut(s)	319, 790, 1123, 1201, 1474, 1524
Hpy188I	TCNGA	3 cut(s)	592, 715, 1018
Hpy188III	TCNNGA	5 cut(s)	173, 805, 950, 1266, 1358
Hpy8I	GTNNAC	6 cut(s)	319, 790, 1123, 1201, 1474, 1524
Hpy99I	CGWCG	2 cut(s)	579, 1142
HpyAV	CCTTC	1 cut(s)	772
HpyCH4III	ACNGT	9 cut(s)	257, 316, 332, 1037, 1120, 1147, 1207, 1339, 1528
HpyCH4IV	ACGT	4 cut(s)	465, 774, 1071, 1301
HpyF10VI	GCNNNNNNNGC	4 cut(s)	212, 278, 725, 1511
HpyF3I	CTNAG	1 cut(s)	497
HpySE526I	ACGT	4 cut(s)	465, 774, 1071, 1301
Hsp92II	CATG	7 cut(s)	84, 199, 372, 405, 835, 958, 1438
HspAI	GCGC	2 cut(s)	1214, 1446
Kzo9I	GATC	4 cut(s)	148, 625, 1018, 1365
LmnI	GCTCC	6 cut(s)	298, 347, 1013, 1019, 1158, 1451
Lsp1109I	GCAGC	5 cut(s)	36, 621, 715, 1053, 1264
LweI	GCATC	4 cut(s)	389, 483, 715, 844
MaeI	CTAG	4 cut(s)	146, 435, 1221, 1332
MaeII	ACGT	4 cut(s)	465, 774, 1071, 1301
MaeIII	GTNAC	7 cut(s)	152, 173, 257, 466, 865, 1052, 1207
MalI	GATC	4 cut(s)	150, 627, 1020, 1367
MbiI	CCGCTC	1 cut(s)	1008
MboI	GATC	4 cut(s)	148, 625, 1018, 1365
MboII	GAAGA	6 cut(s)	29, 525, 582, 835, 1021, 1360
MhlI	GDGCHC	2 cut(s)	303, 1155
MluCI	AATT	6 cut(s)	697, 708, 1088, 1159, 1261, 1341
Mph1103I	ATGCAT	1 cut(s)	16
MseI	TTAA	5 cut(s)	123, 186, 210, 810, 1296
MslI	CAYNNNNRTG	1 cut(s)	773
MspA1I	CMGCKG	2 cut(s)	623, 1586
MspI	CCGG	9 cut(s)	693, 930, 975, 1011, 1443, 1482, 1515, 1545, 1562
MspR9I	CCNGG	4 cut(s)	283, 694, 1482, 1563
MvaI	CCWGG	1 cut(s)	283
MvnI	CGCG	3 cut(s)	1098, 1175, 1267
MwoI	GCNNNNNNNGC	4 cut(s)	212, 278, 725, 1511
NciI	CCSGG	3 cut(s)	694, 1482, 1563
NcoI	CCATGG	1 cut(s)	195
NdeII	GATC	4 cut(s)	148, 625, 1018, 1365
NlaIII	CATG	7 cut(s)	84, 199, 372, 405, 835, 958, 1438
NlaIV	GGNNCC	6 cut(s)	280, 343, 602, 691, 1015, 1567
NmuCI	GTSAC	6 cut(s)	152, 173, 257, 466, 865, 1207
NruI	TCGCGA	1 cut(s)	1267
NsiI	ATGCAT	1 cut(s)	16
NspI	RCATGY	1 cut(s)	405
NspV	TTCGAA	1 cut(s)	963
PaeR7I	CTCGAG	1 cut(s)	267
PcsI	WCGNNNNNNNCGW	4 cut(s)	312, 571, 1119, 1298
PfeI	GAWTC	4 cut(s)	69, 1269, 1550, 1558
PflMI	CCANNNNNTGG	1 cut(s)	997
PfoI	TCCNGGA	1 cut(s)	692
PkrI	GCNGC	7 cut(s)	51, 622, 636, 730, 1043, 1136, 1279
PmaCI	CACGTG	1 cut(s)	775
PmlI	CACGTG	1 cut(s)	775
Ppu21I	YACGTR	2 cut(s)	775, 1072
PsiI	TTATAA	1 cut(s)	1496
Psp124BI	GAGCTC	1 cut(s)	303
Psp6I	CCWGG	1 cut(s)	281
PspCI	CACGTG	1 cut(s)	775
PspEI	GGTNACC	2 cut(s)	257, 1052
PspGI	CCWGG	1 cut(s)	281
PspN4I	GGNNCC	6 cut(s)	280, 343, 602, 691, 1015, 1567
PspPI	GGNCC	8 cut(s)	199, 279, 656, 690, 977, 1224, 1479, 1566
PspXI	VCTCGAGB	1 cut(s)	267
PstI	CTGCAG	2 cut(s)	54, 1282
PstNI	CAGNNNCTG	1 cut(s)	623
PvuII	CAGCTG	1 cut(s)	1586
RruI	TCGCGA	1 cut(s)	1267
RsaI	GTAC	5 cut(s)	734, 1304, 1328, 1433, 1459
RsaNI	GTAC	5 cut(s)	733, 1303, 1327, 1432, 1458
RseI	CAYNNNNRTG	1 cut(s)	773
SacI	GAGCTC	1 cut(s)	303
SalI	GTCGAC	1 cut(s)	1121
SaqAI	TTAA	5 cut(s)	123, 186, 210, 810, 1296
SatI	GCNGC	7 cut(s)	50, 621, 635, 729, 1042, 1135, 1278
Sau3AI	GATC	4 cut(s)	148, 625, 1018, 1365
Sau96I	GGNCC	8 cut(s)	199, 279, 656, 690, 977, 1224, 1479, 1566
ScaI	AGTACT	1 cut(s)	1328
ScrFI	CCNGG	4 cut(s)	283, 694, 1482, 1563
SduI	GDGCHC	2 cut(s)	303, 1155
SfaNI	GCATC	4 cut(s)	389, 483, 715, 844
SfcI	CTRYAG	3 cut(s)	50, 740, 1278
Sfr274I	CTCGAG	1 cut(s)	267
SfuI	TTCGAA	1 cut(s)	963
SinI	GGWCC	6 cut(s)	199, 656, 690, 977, 1224, 1566
SlaI	CTCGAG	1 cut(s)	267
SmiMI	CAYNNNNRTG	1 cut(s)	773
SmlI	CTYRAG	3 cut(s)	102, 267, 948
SmoI	CTYRAG	3 cut(s)	102, 267, 948
Sse9I	AATT	6 cut(s)	697, 708, 1088, 1159, 1261, 1341
SsiI	CCGC	5 cut(s)	621, 654, 1006, 1096, 1134
SspMI	CTAG	4 cut(s)	146, 435, 1221, 1332
SstI	GAGCTC	1 cut(s)	303
StyD4I	CCNGG	4 cut(s)	281, 692, 1480, 1561
StyI	CCWWGG	2 cut(s)	195, 273
TaaI	ACNGT	9 cut(s)	257, 316, 332, 1037, 1120, 1147, 1207, 1339, 1528
TaiI	ACGT	4 cut(s)	468, 777, 1074, 1304
TaqI	TCGA	7 cut(s)	268, 628, 644, 804, 852, 963, 1122
TaqII	GACCGA	1 cut(s)	1241
TasI	AATT	6 cut(s)	697, 708, 1088, 1159, 1261, 1341
TatI	WGTACW	2 cut(s)	1326, 1431
TauI	GCSGC	2 cut(s)	623, 1137
TfiI	GAWTC	4 cut(s)	69, 1269, 1550, 1558
Tru1I	TTAA	5 cut(s)	123, 186, 210, 810, 1296
Tru9I	TTAA	5 cut(s)	123, 186, 210, 810, 1296
TscAI	CASTG	3 cut(s)	79, 976, 1512
TseFI	GTSAC	6 cut(s)	152, 173, 257, 466, 865, 1207
TseI	GCWGC	5 cut(s)	49, 634, 728, 1041, 1277
Tsp45I	GTSAC	6 cut(s)	152, 173, 257, 466, 865, 1207
TspDTI	ATGAA	8 cut(s)	525, 826, 836, 1117, 1247, 1251, 1361, 1451
TspGWI	ACGGA	1 cut(s)	1377
TspRI	CASTG	3 cut(s)	79, 976, 1512
Van91I	CCANNNNNTGG	1 cut(s)	997
VpaK11BI	GGWCC	6 cut(s)	199, 656, 690, 977, 1224, 1566
XceI	RCATGY	1 cut(s)	405
XhoI	CTCGAG	1 cut(s)	267
XmiI	GTMKAC	1 cut(s)	1122
XspI	CTAG	4 cut(s)	146, 435, 1221, 1332
ZrmI	AGTACT	1 cut(s)	1328
Zsp2I	ATGCAT	1 cut(s)	16

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.