Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0013784

Physical Location & Seq

Genomic Coordinates Forward (+)

1569789 .. 1572205

Gene Structure 2417 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1022000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1614 bp

ATGGAGTTCAATGCATATTTTCTTGTGTTCTTTTCTCTTCTATTCATGCTGCAGTTTAGTTCTTGTAGAATCACTGCACCATGCAGTCAAACCCCATACCCTCAAATGTGTCGCCATTACATTAATACTAACCTCTTATCAACCCTAGATCGTCACCAAAGGTTTGCATTTCGTGACTTGGTACTTAAAGTGTCCATGGACCAAGCCATTGAAGCTCATCAACTTGTCTTGTCCTTGAACTTGAACTCATTGGACGCTGACCATAATCGAGCCAAGGGGGCCTGGAATGACTGCTTGGAGCTCTACGAGGACACCGTTGACAAGCTCAACCGTTCGATCGGCTCCACCAATGCCCTTGATGTCAATACATGGTTGAGTGCATCGCTTACTAATCAACAGACATGCCAAAATGGTTTCAAAAACCTCAACATTCCTAGCTCTCATTTGCAGTCTTTCCCAAACTACGTCACGCTCTCAACCAACTTGTCCAAGATGCTTAGCAACTCACTATCAATACACAAGGTCTCAACTTCTTCATCAGCTCTACATTTTAGCAAACAAGTTCGTGGAAGACGACGGCGTTTGCTTTCGGATGCTGGGTTCCCAGAATGGGTTTCAGCCGCTGATCGAATGTTACTCCAATCGATAAGTAGCGGACCAAACGCTGATATGGTAGTAGCACAAGACGGGTCCGGGAATTACAAGTCAATTTCTGAAGCTGTGGATGCTGCGTACAAGCTACAGGGTGGGGCGACTAAAAGGTTTGTCATACACGTGAAGGCAGGAGTTTACAGAGAAAATATCGAGATTAAGAGGACAATGAAGAACATCATGTTCATTGGAGATGGTATTGATGCAACGATTGTCACAGGGAATGAAAATGTCCAAGATGGTTCAACTACATATCGCTCTGCCACATTTGGGGCTAGCGGCGATGGCTTTATTGCTCAAGACATGACATTCGAAAACACAGCCGGACCACAGAAATACCAAGCCGTGGCACTCCGCTCCGGCTCCGACCATTCAGTCTTCTACCGTTGCAGCTTCAAGGGTTACCAAGACACGTTATACGTGTATTCCCAACGCCAATTCTACCGCGACTGCGACATATACGGCACAATCGACTTCATCTTCGGCGACGCCACGGTTGTGCTCCAAAATTGCAACATCTACGCGAGGAAACCAATGAACAACCAAGTAAACACCGTGACAGCGCAGTCTAGGACCGACCCGAATGAAAACACAGGCATTGTTATTCATAATTCACGAATCACGGCTGCACAAGATTTGAGACCTGTACAAGGTTCGTTTAAGACGTACTTGGGTAGGCCGTGGAAAAAGTACTCTAGGACGGTAATTATGAACAGTAATCTTGATGGGTTGATCAATCCTGATGGTTGGTTTCCGTGGAGTGGAAGCTTTGCTCTCTCCACACTTTATTACGGGGAGTACATGAACTCCGGCTCTGGAGCTGGTACTGGAGGGAGGGTCAACTGGCCCGGTTATCGTGTTATAACGAGTGCGGTGGAGGCCGGGAAGTTTACGGTTGGGAACTTTTTGGCCGGGGATTCGTGGATTCCGGGGACCGGAGTTCCATATACAGCTGGTCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

538

Amino Acids

59.56

Weight (kDa)

8.73

Isoelectric Point (pI)

33.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	25 - 172	3.8e-30	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	223 - 523	3e-144	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013784)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23200
fragaria_vesca	FvH4_4g33970
malus_domestica	MD16G1022000.v1.1 MD16G1022100.v1.1 MD16G1022200.v1.1
prunus_persica	Prupe.1G330800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0443501
rosa_laevigata	RLG00000005923
rosa_multiflora	Rmu_sc0001118.1_g000030
rosa_roxburghii	Rroxscaffold_5G00384040
rosa_rugosa	Rorug04G0346700
rosa_samantha	Rh4AG402400 Rh4BG413900 Rh4CG429600 Rh4DG409600 Rh4DG410900
rosa_wichuraiana	Rw4G034690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1514
AasI	GACNNNNNNGTC	2 cut(s)	1025, 1216
AccB7I	CCANNNNNTGG	1 cut(s)	997
AccBSI	CCGCTC	1 cut(s)	1008
AccII	CGCG	2 cut(s)	1098, 1175
AciI	CCGC	6 cut(s)	621, 654, 930, 1006, 1096, 1523
AcoI	YGGCCR	1 cut(s)	1560
AcuI	CTGAAG	1 cut(s)	735
AcvI	CACGTG	1 cut(s)	775
AcyI	GRCGYC	1 cut(s)	1140
AfaI	GTAC	7 cut(s)	183, 734, 1299, 1319, 1343, 1451, 1477
AfiI	CCNNNNNNNGG	6 cut(s)	610, 921, 997, 1301, 1412, 1586
AflIII	ACRYGT	3 cut(s)	772, 1062, 1071
AgsI	TTSAA	7 cut(s)	10, 212, 238, 244, 418, 897, 1048
AjnI	CCWGG	1 cut(s)	281
AjuI	GAANNNNNNNTTGG	2 cut(s)	278, 310
AleI	CACNNNNGTG	1 cut(s)	1148
AloI	GAACNNNNNNTCC	2 cut(s)	584, 616
Alw21I	GWGCWC	2 cut(s)	303, 1155
Alw26I	GTCTC	2 cut(s)	529, 1285
AlwNI	CAGNNNCTG	1 cut(s)	623
AoxI	GGCC	5 cut(s)	279, 1328, 1496, 1530, 1560
ApeKI	GCWGC	4 cut(s)	49, 728, 1041, 1277
ArsI	GACNNNNNNTTYG	4 cut(s)	944, 976, 1115, 1147
AseI	ATTAAT	1 cut(s)	123
AspLEI	GCGC	1 cut(s)	1216
AspS9I	GGNCC	8 cut(s)	199, 279, 656, 690, 977, 1224, 1497, 1584
AsuC2I	CCSGG	5 cut(s)	694, 1500, 1534, 1564, 1581
AsuHPI	GGTGA	1 cut(s)	146
AsuII	TTCGAA	1 cut(s)	963
AsuNHI	GCTAGC	1 cut(s)	926
AvaII	GGWCC	6 cut(s)	199, 656, 690, 977, 1224, 1584
BanII	GRGCYC	1 cut(s)	303
BbrPI	CACGTG	1 cut(s)	775
BbsI	GAAGAC	2 cut(s)	577, 1021
Bbv12I	GWGCWC	2 cut(s)	303, 1155
BbvI	GCAGC	4 cut(s)	36, 715, 1053, 1264
BccI	CCATC	5 cut(s)	839, 884, 929, 1370, 1388
BceAI	ACGGC	5 cut(s)	593, 980, 1129, 1290, 1315
BciT130I	CCWGG	1 cut(s)	283
BclI	TGATCA	1 cut(s)	1383
BcnI	CCSGG	5 cut(s)	694, 1500, 1534, 1564, 1581
BcoDI	GTCTC	2 cut(s)	529, 1285
BfaI	CTAG	5 cut(s)	146, 435, 927, 1221, 1347
BfmI	CTRYAG	2 cut(s)	50, 740
BglI	GCCNNNNNGGC	1 cut(s)	278
BisI	GCNGC	6 cut(s)	50, 621, 729, 931, 1042, 1278
BlpI	GCTNAGC	1 cut(s)	497
BlsI	GCNGC	6 cut(s)	51, 622, 730, 932, 1043, 1279
BmcAI	AGTACT	1 cut(s)	1343
Bme1390I	CCNGG	6 cut(s)	283, 694, 1500, 1534, 1564, 1581
Bme18I	GGWCC	6 cut(s)	199, 656, 690, 977, 1224, 1584
BmgT120I	GGNCC	8 cut(s)	199, 279, 656, 690, 977, 1224, 1497, 1584
BmiI	GGNNCC	6 cut(s)	280, 343, 602, 691, 1015, 1585
BmrFI	CCNGG	6 cut(s)	283, 694, 1500, 1534, 1564, 1581
BmsI	GCATC	5 cut(s)	389, 483, 583, 715, 844
BmtI	GCTAGC	1 cut(s)	930
BpiI	GAAGAC	2 cut(s)	577, 1021
BpmI	CTGGAG	2 cut(s)	1488, 1500
Bpu1102I	GCTNAGC	1 cut(s)	497
Bpu14I	TTCGAA	1 cut(s)	963
BpuEI	CTTGAG	1 cut(s)	933
BpuMI	CCSGG	5 cut(s)	694, 1500, 1534, 1564, 1581
Bsa29I	ATCGAT	1 cut(s)	644
BsaAI	YACGTR	2 cut(s)	775, 1072
BsaHI	GRCGYC	1 cut(s)	1140
BsaI	GGTCTC	2 cut(s)	529, 1285
BsaJI	CCNNGG	8 cut(s)	195, 273, 996, 1143, 1331, 1406, 1563, 1580
BsaWI	WCCGGW	1 cut(s)	1586
BsaXI	ACNNNNNCTCC	4 cut(s)	1442, 1472, 1473, 1503
Bsc4I	CCNNNNNNNGG	6 cut(s)	610, 921, 997, 1301, 1412, 1586
Bse1I	ACTGG	2 cut(s)	1483, 1499
BseBI	CCWGG	1 cut(s)	283
BseCI	ATCGAT	1 cut(s)	644
BseDI	CCNNGG	8 cut(s)	195, 273, 996, 1143, 1331, 1406, 1563, 1580
BseGI	GGATG	2 cut(s)	598, 730
BseLI	CCNNNNNNNGG	6 cut(s)	610, 921, 997, 1301, 1412, 1586
BseNI	ACTGG	2 cut(s)	1483, 1499
BseXI	GCAGC	4 cut(s)	36, 715, 1053, 1264
BseYI	CCCAGC	1 cut(s)	596
BsgI	GTGCAG	2 cut(s)	60, 1263
Bsh1236I	CGCG	2 cut(s)	1098, 1175
Bsh1285I	CGRYCG	1 cut(s)	339
BshFI	GGCC	5 cut(s)	281, 1330, 1498, 1532, 1562
BshVI	ATCGAT	1 cut(s)	644
BsiEI	CGRYCG	1 cut(s)	339
BsiHKAI	GWGCWC	2 cut(s)	303, 1155
BsiSI	CCGG	9 cut(s)	693, 975, 1011, 1461, 1500, 1533, 1563, 1580, 1587
BslFI	GGGAC	1 cut(s)	1597
BslI	CCNNNNNNNGG	6 cut(s)	610, 921, 997, 1301, 1412, 1586
BsmAI	GTCTC	2 cut(s)	529, 1285
BsmFI	GGGAC	1 cut(s)	1597
BsnI	GGCC	5 cut(s)	281, 1330, 1498, 1532, 1562
Bso31I	GGTCTC	2 cut(s)	529, 1285
Bsp119I	TTCGAA	1 cut(s)	963
Bsp1286I	GDGCHC	2 cut(s)	303, 1155
Bsp1407I	TGTACA	1 cut(s)	1297
Bsp143I	GATC	4 cut(s)	148, 336, 625, 1383
Bsp1720I	GCTNAGC	1 cut(s)	497
Bsp19I	CCATGG	1 cut(s)	195
BspACI	CCGC	6 cut(s)	621, 654, 930, 1006, 1096, 1523
BspANI	GGCC	5 cut(s)	281, 1330, 1498, 1532, 1562
BspDI	ATCGAT	1 cut(s)	644
BspFNI	CGCG	2 cut(s)	1098, 1175
BspLI	GGNNCC	6 cut(s)	280, 343, 602, 691, 1015, 1585
BspMAI	CTGCAG	1 cut(s)	54
BspOI	GCTAGC	1 cut(s)	930
BspT104I	TTCGAA	1 cut(s)	963
BspTNI	GGTCTC	2 cut(s)	529, 1285
BsrBI	CCGCTC	1 cut(s)	1008
BsrGI	TGTACA	1 cut(s)	1297
BsrI	ACTGG	2 cut(s)	1483, 1499
BssECI	CCNNGG	8 cut(s)	195, 273, 996, 1143, 1331, 1406, 1563, 1580
BssMI	GATC	4 cut(s)	148, 336, 625, 1383
BssNI	GRCGYC	1 cut(s)	1140
BssT1I	CCWWGG	2 cut(s)	195, 273
Bst2UI	CCWGG	1 cut(s)	283
Bst4CI	ACNGT	8 cut(s)	316, 332, 1037, 1147, 1207, 1354, 1367, 1546
Bst6I	CTCTTC	1 cut(s)	42
BstACI	GRCGYC	1 cut(s)	1140
BstAUI	TGTACA	1 cut(s)	1297
BstBAI	YACGTR	2 cut(s)	775, 1072
BstBI	TTCGAA	1 cut(s)	963
BstC8I	GCNNGC	1 cut(s)	928
BstDEI	CTNAG	1 cut(s)	497
BstDSI	CCRYGG	5 cut(s)	195, 996, 1143, 1331, 1406
BstEII	GGTNACC	1 cut(s)	1052
BstENI	CCTNNNNNAGG	1 cut(s)	1299
BstF5I	GGATG	2 cut(s)	598, 730
BstFNI	CGCG	2 cut(s)	1098, 1175
BstHHI	GCGC	1 cut(s)	1216
BstKTI	GATC	4 cut(s)	151, 339, 628, 1386
BstMAI	GTCTC	2 cut(s)	529, 1285
BstMBI	GATC	4 cut(s)	148, 336, 625, 1383
BstMCI	CGRYCG	1 cut(s)	339
BstMWI	GCNNNNNNNGC	5 cut(s)	212, 278, 725, 936, 1529
BstNI	CCWGG	1 cut(s)	283
BstNSI	RCATGY	1 cut(s)	405
BstPI	GGTNACC	1 cut(s)	1052
BstSCI	CCNGG	6 cut(s)	281, 692, 1498, 1532, 1562, 1579
BstSFI	CTRYAG	2 cut(s)	50, 740
BstUI	CGCG	2 cut(s)	1098, 1175
BstV1I	GCAGC	4 cut(s)	36, 715, 1053, 1264
BstV2I	GAAGAC	2 cut(s)	577, 1021
Bsu15I	ATCGAT	1 cut(s)	644
BsuRI	GGCC	5 cut(s)	281, 1330, 1498, 1532, 1562
BsuTUI	ATCGAT	1 cut(s)	644
BtgI	CCRYGG	5 cut(s)	195, 996, 1143, 1331, 1406
BtgZI	GCGATG	2 cut(s)	366, 948
BtsCI	GGATG	2 cut(s)	598, 730
BtsI	GCAGTG	1 cut(s)	72
BtsIMutI	CAGTG	1 cut(s)	72
Cac8I	GCNNGC	1 cut(s)	928
CaiI	CAGNNNCTG	1 cut(s)	623
CfoI	GCGC	1 cut(s)	1216
Cfr13I	GGNCC	8 cut(s)	199, 279, 656, 690, 977, 1224, 1497, 1584
ClaI	ATCGAT	1 cut(s)	644
CseI	GACGC	2 cut(s)	263, 1148
Csp6I	GTAC	7 cut(s)	182, 733, 1298, 1318, 1342, 1450, 1476
CviAII	CATG	8 cut(s)	46, 81, 196, 369, 402, 832, 955, 1453
CviQI	GTAC	7 cut(s)	182, 733, 1298, 1318, 1342, 1450, 1476
DdeI	CTNAG	1 cut(s)	497
DpnI	GATC	4 cut(s)	150, 338, 627, 1385
DpnII	GATC	4 cut(s)	148, 336, 625, 1383
DrdI	GACNNNNNNGTC	2 cut(s)	1025, 1216
DseDI	GACNNNNNNGTC	2 cut(s)	1025, 1216
EaeI	YGGCCR	1 cut(s)	1560
Eam1104I	CTCTTC	1 cut(s)	42
EarI	CTCTTC	1 cut(s)	42
Ecl136II	GAGCTC	1 cut(s)	301
Eco130I	CCWWGG	2 cut(s)	195, 273
Eco24I	GRGCYC	1 cut(s)	303
Eco31I	GGTCTC	2 cut(s)	529, 1285
Eco47I	GGWCC	6 cut(s)	199, 656, 690, 977, 1224, 1584
Eco53kI	GAGCTC	1 cut(s)	301
Eco57I	CTGAAG	1 cut(s)	735
Eco72I	CACGTG	1 cut(s)	775
Eco91I	GGTNACC	1 cut(s)	1052
EcoICRI	GAGCTC	1 cut(s)	301
EcoNI	CCTNNNNNAGG	1 cut(s)	1299
EcoO109I	RGGNCCY	1 cut(s)	279
EcoO65I	GGTNACC	1 cut(s)	1052
EcoRII	CCWGG	1 cut(s)	281
EcoT14I	CCWWGG	2 cut(s)	195, 273
EcoT22I	ATGCAT	1 cut(s)	16
EcoT38I	GRGCYC	1 cut(s)	303
ErhI	CCWWGG	2 cut(s)	195, 273
FaeI	CATG	8 cut(s)	49, 84, 199, 372, 405, 835, 958, 1456
FalI	AAGNNNNNCTT	2 cut(s)	1304, 1336
FaqI	GGGAC	1 cut(s)	1597
FatI	CATG	8 cut(s)	45, 80, 195, 368, 401, 831, 954, 1452
FbaI	TGATCA	1 cut(s)	1383
Fnu4HI	GCNGC	6 cut(s)	50, 621, 729, 931, 1042, 1278
FokI	GGATG	2 cut(s)	605, 737
FriOI	GRGCYC	1 cut(s)	303
Fsp4HI	GCNGC	6 cut(s)	50, 621, 729, 931, 1042, 1278
FspBI	CTAG	5 cut(s)	146, 435, 927, 1221, 1347
GlaI	GCGC	1 cut(s)	1215
GluI	GCNGC	6 cut(s)	50, 621, 729, 931, 1042, 1278
GsaI	CCCAGC	1 cut(s)	600
GsuI	CTGGAG	2 cut(s)	1488, 1500
HaeIII	GGCC	5 cut(s)	281, 1330, 1498, 1532, 1562
HapII	CCGG	9 cut(s)	693, 975, 1011, 1461, 1500, 1533, 1563, 1580, 1587
HgaI	GACGC	2 cut(s)	263, 1148
HhaI	GCGC	1 cut(s)	1216
Hin1I	GRCGYC	1 cut(s)	1140
Hin1II	CATG	8 cut(s)	49, 84, 199, 372, 405, 835, 958, 1456
Hin6I	GCGC	1 cut(s)	1214
HinP1I	GCGC	1 cut(s)	1214
HincII	GTYRAC	2 cut(s)	319, 1492
HindII	GTYRAC	2 cut(s)	319, 1492
HindIII	AAGCTT	1 cut(s)	1417
HinfI	GANTC	4 cut(s)	69, 1269, 1568, 1576
HpaII	CCGG	9 cut(s)	693, 975, 1011, 1461, 1500, 1533, 1563, 1580, 1587
HphI	GGTGA	1 cut(s)	146
Hpy166II	GTNNAC	5 cut(s)	319, 790, 1201, 1492, 1542
Hpy188I	TCNGA	3 cut(s)	592, 715, 1018
Hpy188III	TCNNGA	7 cut(s)	173, 805, 950, 1266, 1373, 1391, 1467
Hpy8I	GTNNAC	5 cut(s)	319, 790, 1201, 1492, 1542
Hpy99I	CGWCG	2 cut(s)	579, 1142
HpyAV	CCTTC	1 cut(s)	772
HpyCH4III	ACNGT	8 cut(s)	316, 332, 1037, 1147, 1207, 1354, 1367, 1546
HpyCH4IV	ACGT	5 cut(s)	465, 774, 1064, 1071, 1316
HpyF10VI	GCNNNNNNNGC	5 cut(s)	212, 278, 725, 936, 1529
HpyF3I	CTNAG	1 cut(s)	497
HpySE526I	ACGT	5 cut(s)	465, 774, 1064, 1071, 1316
Hsp92I	GRCGYC	1 cut(s)	1140
Hsp92II	CATG	8 cut(s)	49, 84, 199, 372, 405, 835, 958, 1456
HspAI	GCGC	1 cut(s)	1214
Ksp22I	TGATCA	1 cut(s)	1383
Kzo9I	GATC	4 cut(s)	148, 336, 625, 1383
LmnI	GCTCC	6 cut(s)	298, 347, 1013, 1019, 1158, 1469
Lsp1109I	GCAGC	4 cut(s)	36, 715, 1053, 1264
LweI	GCATC	5 cut(s)	389, 483, 583, 715, 844
MaeI	CTAG	5 cut(s)	146, 435, 927, 1221, 1347
MaeII	ACGT	5 cut(s)	465, 774, 1064, 1071, 1316
MaeIII	GTNAC	7 cut(s)	152, 173, 466, 633, 865, 1052, 1207
MalI	GATC	4 cut(s)	150, 338, 627, 1385
MbiI	CCGCTC	1 cut(s)	1008
MboI	GATC	4 cut(s)	148, 336, 625, 1383
MboII	GAAGA	6 cut(s)	29, 525, 582, 835, 1021, 1123
MhlI	GDGCHC	2 cut(s)	303, 1155
MluCI	AATT	6 cut(s)	697, 708, 1088, 1159, 1261, 1356
MmeI	TCCRAC	1 cut(s)	1041
MnlI	CCTC	9 cut(s)	111, 143, 301, 434, 807, 1170, 1475, 1479, 1522
Mph1103I	ATGCAT	1 cut(s)	16
MseI	TTAA	4 cut(s)	123, 186, 810, 1311
MslI	CAYNNNNRTG	2 cut(s)	773, 1148
MspA1I	CMGCKG	2 cut(s)	623, 1604
MspI	CCGG	9 cut(s)	693, 975, 1011, 1461, 1500, 1533, 1563, 1580, 1587
MspR9I	CCNGG	6 cut(s)	283, 694, 1500, 1534, 1564, 1581
MvaI	CCWGG	1 cut(s)	283
MvnI	CGCG	2 cut(s)	1098, 1175
MwoI	GCNNNNNNNGC	5 cut(s)	212, 278, 725, 936, 1529
NciI	CCSGG	5 cut(s)	694, 1500, 1534, 1564, 1581
NcoI	CCATGG	1 cut(s)	195
NdeII	GATC	4 cut(s)	148, 336, 625, 1383
NheI	GCTAGC	1 cut(s)	926
NlaIII	CATG	8 cut(s)	49, 84, 199, 372, 405, 835, 958, 1456
NlaIV	GGNNCC	6 cut(s)	280, 343, 602, 691, 1015, 1585
NmuCI	GTSAC	5 cut(s)	152, 173, 466, 865, 1207
NsiI	ATGCAT	1 cut(s)	16
NspI	RCATGY	1 cut(s)	405
NspV	TTCGAA	1 cut(s)	963
OliI	CACNNNNGTG	1 cut(s)	1148
PcsI	WCGNNNNNNNCGW	4 cut(s)	312, 571, 1119, 1313
PfeI	GAWTC	4 cut(s)	69, 1269, 1568, 1576
PflMI	CCANNNNNTGG	1 cut(s)	997
PfoI	TCCNGGA	1 cut(s)	692
PkrI	GCNGC	6 cut(s)	51, 622, 730, 932, 1043, 1279
Ple19I	CGATCG	1 cut(s)	339
PmaCI	CACGTG	1 cut(s)	775
PmlI	CACGTG	1 cut(s)	775
Ppu21I	YACGTR	2 cut(s)	775, 1072
PshBI	ATTAAT	1 cut(s)	123
PsiI	TTATAA	1 cut(s)	1514
Psp124BI	GAGCTC	1 cut(s)	303
Psp6I	CCWGG	1 cut(s)	281
PspCI	CACGTG	1 cut(s)	775
PspEI	GGTNACC	1 cut(s)	1052
PspFI	CCCAGC	1 cut(s)	596
PspGI	CCWGG	1 cut(s)	281
PspN4I	GGNNCC	6 cut(s)	280, 343, 602, 691, 1015, 1585
PspPI	GGNCC	8 cut(s)	199, 279, 656, 690, 977, 1224, 1497, 1584
PstI	CTGCAG	1 cut(s)	54
PstNI	CAGNNNCTG	1 cut(s)	623
PvuI	CGATCG	1 cut(s)	339
PvuII	CAGCTG	1 cut(s)	1604
RsaI	GTAC	7 cut(s)	183, 734, 1299, 1319, 1343, 1451, 1477
RsaNI	GTAC	7 cut(s)	182, 733, 1298, 1318, 1342, 1450, 1476
RseI	CAYNNNNRTG	2 cut(s)	773, 1148
SacI	GAGCTC	1 cut(s)	303
SaqAI	TTAA	4 cut(s)	123, 186, 810, 1311
SatI	GCNGC	6 cut(s)	50, 621, 729, 931, 1042, 1278
Sau3AI	GATC	4 cut(s)	148, 336, 625, 1383
Sau96I	GGNCC	8 cut(s)	199, 279, 656, 690, 977, 1224, 1497, 1584
ScaI	AGTACT	1 cut(s)	1343
ScrFI	CCNGG	6 cut(s)	283, 694, 1500, 1534, 1564, 1581
SduI	GDGCHC	2 cut(s)	303, 1155
SfaNI	GCATC	5 cut(s)	389, 483, 583, 715, 844
SfcI	CTRYAG	2 cut(s)	50, 740
SfuI	TTCGAA	1 cut(s)	963
SinI	GGWCC	6 cut(s)	199, 656, 690, 977, 1224, 1584
SmiMI	CAYNNNNRTG	2 cut(s)	773, 1148
SmlI	CTYRAG	1 cut(s)	948
SmoI	CTYRAG	1 cut(s)	948
Sse9I	AATT	6 cut(s)	697, 708, 1088, 1159, 1261, 1356
SsiI	CCGC	6 cut(s)	621, 654, 930, 1006, 1096, 1523
SspMI	CTAG	5 cut(s)	146, 435, 927, 1221, 1347
SstI	GAGCTC	1 cut(s)	303
StyD4I	CCNGG	6 cut(s)	281, 692, 1498, 1532, 1562, 1579
StyI	CCWWGG	2 cut(s)	195, 273
TaaI	ACNGT	8 cut(s)	316, 332, 1037, 1147, 1207, 1354, 1367, 1546
TaiI	ACGT	5 cut(s)	468, 777, 1067, 1074, 1319
TaqI	TCGA	7 cut(s)	268, 335, 628, 644, 804, 963, 1122
TaqII	GACCGA	1 cut(s)	1241
TasI	AATT	6 cut(s)	697, 708, 1088, 1159, 1261, 1356
TatI	WGTACW	3 cut(s)	1297, 1341, 1449
TauI	GCSGC	2 cut(s)	623, 933
TfiI	GAWTC	4 cut(s)	69, 1269, 1568, 1576
Tru1I	TTAA	4 cut(s)	123, 186, 810, 1311
Tru9I	TTAA	4 cut(s)	123, 186, 810, 1311
TscAI	CASTG	1 cut(s)	79
TseFI	GTSAC	5 cut(s)	152, 173, 466, 865, 1207
TseI	GCWGC	4 cut(s)	49, 728, 1041, 1277
Tsp45I	GTSAC	5 cut(s)	152, 173, 466, 865, 1207
TspGWI	ACGGA	1 cut(s)	1395
TspRI	CASTG	1 cut(s)	79
Van91I	CCANNNNNTGG	1 cut(s)	997
VpaK11BI	GGWCC	6 cut(s)	199, 656, 690, 977, 1224, 1584
VspI	ATTAAT	1 cut(s)	123
XagI	CCTNNNNNAGG	1 cut(s)	1299
XceI	RCATGY	1 cut(s)	405
XspI	CTAG	5 cut(s)	146, 435, 927, 1221, 1347
ZrmI	AGTACT	1 cut(s)	1343
Zsp2I	ATGCAT	1 cut(s)	16

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.