Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0013784

Physical Location & Seq

Genomic Coordinates Forward (+)

63578168 .. 63580545

Gene Structure 2378 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00384040.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1626 bp

ATGGATAGCCTTGTGATCTTAGCCATTTTGATGATCACTTCAAATTTTCTTGTAGTACAAATTGTTTCCATAACAGCATCATGCAGCCAAACCCCATATCCTCAAGTTTGCAACCATTACATTACCAACAACCTCTTATCGACGTTTGATGTTCGCGAGCAAGGGTTTGCATTTCGTAGCCTAGCCCTCAAGGTCACCATGGACCAAGCCATCGAAGCGCATCGACTTGTTTCATCAGCAGTGCACTTGAAGTCATTGTACAACGAGCGAGCCAAGTTGGCGTGGGGTGATTGCTTGGAACTCTACGAGGATACCATTGACAAGCTCAATCGTTCGATGAGTTCCAACAATGCTTTAGACGCTAATACATGGTTAAGTGCATCCATTGCCAATCAACAAACTTGCCAAAACTGTTTTAGCGATCTAAACATTCCTAATTCTCATTTACAATCTTCGTTCCCAAACTTTTACAAGCTAAGCACAGACTTGCCTATGTTGCTTAGCAATTCGCTAGCAATACACAAGGCGTCTAGTACTTCTTCTTCTTCCTCGGCTATGCATTTCAGCAAACAGGTACGAGGCAGACGCTTATTGCTCAAGTCTGATGATGGGTTCCCGGAATGGCTTTCAGCTTCAGATCGAAAGCTTCTCCAGTCGAGCATCGGACCAAAGCCAGCTGATATAGTAGTAGCTCAAGATGGGTCTGGAAATTACAAGTCTATAACTGAAGCTGTGGCTGCAGCCAAGGAAAGTAGTGGTAGTGGGACCAGAAGGGTTGTCATATACGTGAAGGCAGGGGTTTACAAAGAGAATGTTGAGGTTAAGAAGACAATGAAGAAGCTGATGTTTGTCGGAGATGGGATGGATGCAACCATTGTTACCGGGAATAAAAATGCCCAAGATGGCTCTACCACTTTTCGCTCTGCCACTTTCGCTGTTTCCGGCGAAGGCTTCATAGCTCAAGACATGACGTTCGAAAACACGGCCGGACCACAGAAACACCAAGCCGTGGCACTCCGCTCCGGCGCCGACCACTCAGTCTTCTACAGATGCAGCTTCAAAGGCTACCAAGACACCTTATACGTTTACTCCCAACGTCAGTTCTACCGTGACTGCGACATATACGGCACCGTAGACTTCATCTTCGGCGACGCTGCCGCCATCCTCCAAAACTGCAACATCTACGTCAGAAAGCCCATGAGCAACCAGATCAACACCGTGACGGCCCAATCCCGGAGAGACCCTAATGAGAACACGGGCATTATTATCCACAACTGCCGCATAACGGCGGCACCGGACCTGAGAGCCGTGCAGGGCTCGTTCAGGACCTATTTGGGTCGGCCGTGGCAGAAGTATTCAAGGACGGTAATAATGAAGAGTAACCTAGATGGGTTGATTGCGCCACAAGGTTGGTTTCCTTGGAGTGGAAGTTTTGCGTTGGATACGCTTTATTATGGGGAGTATATGAACACCGGGGCTGGTGCTGCTACTGGAGGGAGAGTTAAGTGGCCTGGTTTTCGGGTTATAACGAGTGCGGCGGAGGCGGGGAAGTTCACCGTAGGGAATTTCTTGGCCGGAGACTCCTGGATTCCGGGGACTGGAGTGCCGTTTGAAGCTGGTCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

541

Amino Acids

59.35

Weight (kDa)

8.78

Isoelectric Point (pI)

36.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	23 - 173	5.4e-28	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	227 - 527	3.5e-147	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0013784)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23200
fragaria_vesca	FvH4_4g33970
malus_domestica	MD16G1022000.v1.1 MD16G1022100.v1.1 MD16G1022200.v1.1
prunus_persica	Prupe.1G330800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0443501
rosa_laevigata	RLG00000005923
rosa_multiflora	Rmu_sc0001118.1_g000030
rosa_roxburghii	Rroxscaffold_5G00384040
rosa_rugosa	Rorug04G0346700
rosa_samantha	Rh4AG402400 Rh4BG413900 Rh4CG429600 Rh4DG409600 Rh4DG410900
rosa_wichuraiana	Rw4G034690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1526
AasI	GACNNNNNNGTC	1 cut(s)	1037
AccB1I	GGYRCC	3 cut(s)	1025, 1127, 1291
AccB7I	CCANNNNNTGG	1 cut(s)	1009
AccBSI	CCGCTC	1 cut(s)	1020
AccI	GTMKAC	1 cut(s)	1134
AccII	CGCG	1 cut(s)	156
AciI	CCGC	7 cut(s)	1018, 1158, 1279, 1289, 1535, 1538, 1544
AcoI	YGGCCR	3 cut(s)	984, 1340, 1572
AcsI	RAATTY	2 cut(s)	43, 1564
AcuI	CTGAAG	2 cut(s)	618, 747
AcyI	GRCGYC	2 cut(s)	527, 1026
AfaI	GTAC	4 cut(s)	57, 260, 535, 576
AfiI	CCNNNNNNNGG	5 cut(s)	1009, 1233, 1285, 1424, 1598
AgsI	TTSAA	5 cut(s)	42, 250, 1060, 1359, 1613
AjnI	CCWGG	2 cut(s)	1510, 1583
Alw21I	GWGCWC	1 cut(s)	246
Alw26I	GTCTC	2 cut(s)	1233, 1572
Alw44I	GTGCAC	1 cut(s)	242
AoxI	GGCC	5 cut(s)	984, 1224, 1340, 1508, 1572
ApaLI	GTGCAC	1 cut(s)	242
ApeKI	GCWGC	6 cut(s)	84, 737, 740, 1053, 1154, 1484
ApoI	RAATTY	2 cut(s)	43, 1564
ArsI	GACNNNNNNTTYG	4 cut(s)	956, 988, 1127, 1159
AspLEI	GCGC	3 cut(s)	220, 1028, 1402
AspS9I	GGNCC	7 cut(s)	202, 665, 765, 989, 1225, 1297, 1326
AsuC2I	CCSGG	5 cut(s)	617, 883, 1234, 1474, 1593
AsuHPI	GGTGA	3 cut(s)	187, 299, 1546
AsuII	TTCGAA	1 cut(s)	975
AsuNHI	GCTAGC	1 cut(s)	511
AvaII	GGWCC	6 cut(s)	202, 665, 765, 989, 1297, 1326
BaeGI	GKGCMC	1 cut(s)	246
BanI	GGYRCC	3 cut(s)	1025, 1127, 1291
BanII	GRGCYC	1 cut(s)	1319
BarI	GAAGNNNNNNTAC	4 cut(s)	242, 274, 526, 558
BbsI	GAAGAC	2 cut(s)	833, 1033
Bbv12I	GWGCWC	1 cut(s)	246
BbvI	GCAGC	6 cut(s)	96, 724, 752, 1065, 1141, 1471
BccI	CCATC	8 cut(s)	218, 602, 692, 851, 856, 896, 1169, 1382
BceAI	ACGGC	8 cut(s)	992, 999, 1141, 1239, 1292, 1302, 1327, 1591
BciT130I	CCWGG	2 cut(s)	1512, 1585
BciVI	GTATCC	2 cut(s)	304, 1435
BclI	TGATCA	1 cut(s)	33
BcnI	CCSGG	5 cut(s)	617, 883, 1234, 1474, 1593
BcoDI	GTCTC	2 cut(s)	1233, 1572
BfaI	CTAG	4 cut(s)	182, 512, 531, 1385
BfmI	CTRYAG	2 cut(s)	738, 1045
BfoI	RGCGCY	1 cut(s)	1029
BfuI	GTATCC	2 cut(s)	304, 1435
BglI	GCCNNNNNGGC	1 cut(s)	278
BlpI	GCTNAGC	2 cut(s)	476, 500
BmcAI	AGTACT	1 cut(s)	535
Bme1390I	CCNGG	7 cut(s)	617, 883, 1234, 1474, 1512, 1585, 1593
Bme18I	GGWCC	6 cut(s)	202, 665, 765, 989, 1297, 1326
BmgT120I	GGNCC	7 cut(s)	202, 665, 765, 989, 1225, 1297, 1326
BmiI	GGNNCC	5 cut(s)	614, 766, 1027, 1129, 1293
BmrFI	CCNGG	7 cut(s)	617, 883, 1234, 1474, 1512, 1585, 1593
BmsI	GCATC	6 cut(s)	86, 229, 389, 669, 856, 1040
BmtI	GCTAGC	1 cut(s)	515
BpiI	GAAGAC	2 cut(s)	833, 1033
BpmI	CTGGAG	3 cut(s)	635, 1512, 1620
Bpu1102I	GCTNAGC	2 cut(s)	476, 500
Bpu14I	TTCGAA	1 cut(s)	975
BpuEI	CTTGAG	5 cut(s)	87, 173, 581, 678, 945
BpuMI	CCSGG	5 cut(s)	617, 883, 1234, 1474, 1593
BsaAI	YACGTR	1 cut(s)	787
BsaHI	GRCGYC	2 cut(s)	527, 1026
BsaI	GGTCTC	1 cut(s)	1233
BsaJI	CCNNGG	8 cut(s)	198, 549, 744, 1008, 1343, 1418, 1473, 1592
BsaWI	WCCGGW	1 cut(s)	1294
BsaXI	ACNNNNNCTCC	6 cut(s)	1073, 1103, 1414, 1444, 1485, 1515
Bsc4I	CCNNNNNNNGG	5 cut(s)	1009, 1233, 1285, 1424, 1598
Bse1I	ACTGG	3 cut(s)	652, 1495, 1603
Bse3DI	GCAATG	1 cut(s)	384
BseBI	CCWGG	2 cut(s)	1512, 1585
BseDI	CCNNGG	8 cut(s)	198, 549, 744, 1008, 1343, 1418, 1473, 1592
BseGI	GGATG	4 cut(s)	380, 867, 871, 1161
BseLI	CCNNNNNNNGG	5 cut(s)	1009, 1233, 1285, 1424, 1598
BseMI	GCAATG	1 cut(s)	384
BseMII	CTCAG	2 cut(s)	1050, 1292
BseNI	ACTGG	3 cut(s)	652, 1495, 1603
BseSI	GKGCMC	1 cut(s)	246
BseX3I	CGGCCG	2 cut(s)	984, 1340
BseXI	GCAGC	6 cut(s)	96, 724, 752, 1065, 1141, 1471
BsgI	GTGCAG	1 cut(s)	1331
Bsh1236I	CGCG	1 cut(s)	156
Bsh1285I	CGRYCG	2 cut(s)	987, 1343
BshFI	GGCC	5 cut(s)	986, 1226, 1342, 1510, 1574
BshNI	GGYRCC	3 cut(s)	1025, 1127, 1291
BsiEI	CGRYCG	2 cut(s)	987, 1343
BsiHKAI	GWGCWC	1 cut(s)	246
BslFI	GGGAC	2 cut(s)	778, 1609
BslI	CCNNNNNNNGG	5 cut(s)	1009, 1233, 1285, 1424, 1598
BsmAI	GTCTC	2 cut(s)	1233, 1572
BsmFI	GGGAC	2 cut(s)	778, 1609
BsnI	GGCC	5 cut(s)	986, 1226, 1342, 1510, 1574
Bso31I	GGTCTC	1 cut(s)	1233
Bsp119I	TTCGAA	1 cut(s)	975
Bsp1286I	GDGCHC	2 cut(s)	246, 1319
Bsp1407I	TGTACA	1 cut(s)	258
Bsp143I	GATC	5 cut(s)	15, 33, 421, 637, 1209
Bsp1720I	GCTNAGC	2 cut(s)	476, 500
Bsp19I	CCATGG	1 cut(s)	198
Bsp68I	TCGCGA	1 cut(s)	156
BspACI	CCGC	7 cut(s)	1018, 1158, 1279, 1289, 1535, 1538, 1544
BspANI	GGCC	5 cut(s)	986, 1226, 1342, 1510, 1574
BspCNI	CTCAG	2 cut(s)	1049, 1293
BspFNI	CGCG	1 cut(s)	156
BspLI	GGNNCC	5 cut(s)	614, 766, 1027, 1129, 1293
BspMAI	CTGCAG	1 cut(s)	742
BspOI	GCTAGC	1 cut(s)	515
BspT104I	TTCGAA	1 cut(s)	975
BspT107I	GGYRCC	3 cut(s)	1025, 1127, 1291
BspTNI	GGTCTC	1 cut(s)	1233
BsrBI	CCGCTC	1 cut(s)	1020
BsrDI	GCAATG	1 cut(s)	384
BsrGI	TGTACA	1 cut(s)	258
BsrI	ACTGG	3 cut(s)	652, 1495, 1603
BssECI	CCNNGG	8 cut(s)	198, 549, 744, 1008, 1343, 1418, 1473, 1592
BssMI	GATC	5 cut(s)	15, 33, 421, 637, 1209
BssNI	GRCGYC	2 cut(s)	527, 1026
BssT1I	CCWWGG	3 cut(s)	198, 744, 1418
Bst2UI	CCWGG	2 cut(s)	1512, 1585
Bst4CI	ACNGT	6 cut(s)	413, 1109, 1132, 1219, 1366, 1558
Bst6I	CTCTTC	1 cut(s)	1370
BstACI	GRCGYC	2 cut(s)	527, 1026
BstAPI	GCANNNNNTGC	1 cut(s)	386
BstAUI	TGTACA	1 cut(s)	258
BstBAI	YACGTR	1 cut(s)	787
BstBI	TTCGAA	1 cut(s)	975
BstC8I	GCNNGC	4 cut(s)	158, 270, 513, 675
BstDEI	CTNAG	5 cut(s)	19, 476, 500, 1036, 1301
BstDSI	CCRYGG	3 cut(s)	198, 1008, 1343
BstEII	GGTNACC	1 cut(s)	193
BstF5I	GGATG	4 cut(s)	380, 867, 871, 1161
BstFNI	CGCG	1 cut(s)	156
BstH2I	RGCGCY	1 cut(s)	1029
BstHHI	GCGC	3 cut(s)	220, 1028, 1402
BstKTI	GATC	5 cut(s)	18, 36, 424, 640, 1212
BstMAI	GTCTC	2 cut(s)	1233, 1572
BstMBI	GATC	5 cut(s)	15, 33, 421, 637, 1209
BstMCI	CGRYCG	2 cut(s)	987, 1343
BstNI	CCWGG	2 cut(s)	1512, 1585
BstPI	GGTNACC	1 cut(s)	193
BstSCI	CCNGG	7 cut(s)	615, 881, 1232, 1472, 1510, 1583, 1591
BstSFI	CTRYAG	2 cut(s)	738, 1045
BstSLI	GKGCMC	1 cut(s)	246
BstUI	CGCG	1 cut(s)	156
BstV1I	GCAGC	6 cut(s)	96, 724, 752, 1065, 1141, 1471
BstV2I	GAAGAC	2 cut(s)	833, 1033
BstXI	CCANNNNNNTGG	1 cut(s)	1410
BstZI	CGGCCG	2 cut(s)	984, 1340
BsuI	GTATCC	2 cut(s)	304, 1435
BsuRI	GGCC	5 cut(s)	986, 1226, 1342, 1510, 1574
BtgI	CCRYGG	3 cut(s)	198, 1008, 1343
BtsCI	GGATG	4 cut(s)	380, 867, 871, 1161
BtsI	GCAGTG	1 cut(s)	246
BtsIMutI	CAGTG	1 cut(s)	246
BtuMI	TCGCGA	1 cut(s)	156
Cac8I	GCNNGC	4 cut(s)	158, 270, 513, 675
CfoI	GCGC	3 cut(s)	220, 1028, 1402
Cfr13I	GGNCC	7 cut(s)	202, 665, 765, 989, 1225, 1297, 1326
CseI	GACGC	4 cut(s)	368, 516, 594, 1160
Csp6I	GTAC	4 cut(s)	56, 259, 534, 575
CviAII	CATG	5 cut(s)	81, 199, 369, 967, 1198
CviQI	GTAC	4 cut(s)	56, 259, 534, 575
DdeI	CTNAG	5 cut(s)	19, 476, 500, 1036, 1301
DinI	GGCGCC	1 cut(s)	1027
DpnI	GATC	5 cut(s)	17, 35, 423, 639, 1211
DpnII	GATC	5 cut(s)	15, 33, 421, 637, 1209
DrdI	GACNNNNNNGTC	1 cut(s)	1037
DseDI	GACNNNNNNGTC	1 cut(s)	1037
EaeI	YGGCCR	3 cut(s)	984, 1340, 1572
EagI	CGGCCG	2 cut(s)	984, 1340
Eam1104I	CTCTTC	1 cut(s)	1370
EarI	CTCTTC	1 cut(s)	1370
EciI	GGCGGA	1 cut(s)	1553
EclXI	CGGCCG	2 cut(s)	984, 1340
Eco130I	CCWWGG	3 cut(s)	198, 744, 1418
Eco24I	GRGCYC	1 cut(s)	1319
Eco31I	GGTCTC	1 cut(s)	1233
Eco47I	GGWCC	6 cut(s)	202, 665, 765, 989, 1297, 1326
Eco52I	CGGCCG	2 cut(s)	984, 1340
Eco57I	CTGAAG	2 cut(s)	618, 747
Eco91I	GGTNACC	1 cut(s)	193
EcoO109I	RGGNCCY	1 cut(s)	1326
EcoO65I	GGTNACC	1 cut(s)	193
EcoRII	CCWGG	2 cut(s)	1510, 1583
EcoT14I	CCWWGG	3 cut(s)	198, 744, 1418
EcoT22I	ATGCAT	1 cut(s)	561
EcoT38I	GRGCYC	1 cut(s)	1319
EgeI	GGCGCC	1 cut(s)	1027
EheI	GGCGCC	1 cut(s)	1027
ErhI	CCWWGG	3 cut(s)	198, 744, 1418
FaeI	CATG	5 cut(s)	84, 202, 372, 970, 1201
FaqI	GGGAC	2 cut(s)	778, 1609
FatI	CATG	5 cut(s)	80, 198, 368, 966, 1197
FauI	CCCGC	1 cut(s)	1537
FbaI	TGATCA	1 cut(s)	33
FblI	GTMKAC	1 cut(s)	1134
FokI	GGATG	4 cut(s)	367, 874, 878, 1148
FriOI	GRGCYC	1 cut(s)	1319
FspBI	CTAG	4 cut(s)	182, 512, 531, 1385
GlaI	GCGC	3 cut(s)	219, 1027, 1401
GsuI	CTGGAG	3 cut(s)	635, 1512, 1620
HaeII	RGCGCY	1 cut(s)	1029
HaeIII	GGCC	5 cut(s)	986, 1226, 1342, 1510, 1574
HgaI	GACGC	4 cut(s)	368, 516, 594, 1160
HhaI	GCGC	3 cut(s)	220, 1028, 1402
Hin1I	GRCGYC	2 cut(s)	527, 1026
Hin1II	CATG	5 cut(s)	84, 202, 372, 970, 1201
Hin6I	GCGC	3 cut(s)	218, 1026, 1400
HinP1I	GCGC	3 cut(s)	218, 1026, 1400
HindIII	AAGCTT	1 cut(s)	644
HinfI	GANTC	2 cut(s)	1580, 1588
HphI	GGTGA	3 cut(s)	187, 299, 1546
Hpy166II	GTNNAC	5 cut(s)	244, 802, 1087, 1135, 1554
Hpy188I	TCNGA	5 cut(s)	604, 637, 665, 854, 1190
Hpy188III	TCNNGA	5 cut(s)	155, 695, 705, 962, 1324
Hpy8I	GTNNAC	5 cut(s)	244, 802, 1087, 1135, 1554
Hpy99I	CGWCG	2 cut(s)	145, 1154
HpyAV	CCTTC	3 cut(s)	765, 784, 941
HpyCH4III	ACNGT	6 cut(s)	413, 1109, 1132, 1219, 1366, 1558
HpyCH4IV	ACGT	6 cut(s)	143, 786, 971, 1083, 1096, 1185
HpyF3I	CTNAG	5 cut(s)	19, 476, 500, 1036, 1301
HpySE526I	ACGT	6 cut(s)	143, 786, 971, 1083, 1096, 1185
Hsp92I	GRCGYC	2 cut(s)	527, 1026
Hsp92II	CATG	5 cut(s)	84, 202, 372, 970, 1201
HspAI	GCGC	3 cut(s)	218, 1026, 1400
KasI	GGCGCC	1 cut(s)	1025
Ksp22I	TGATCA	1 cut(s)	33
Kzo9I	GATC	5 cut(s)	15, 33, 421, 637, 1209
LmnI	GCTCC	1 cut(s)	1025
Lsp1109I	GCAGC	6 cut(s)	96, 724, 752, 1065, 1141, 1471
LweI	GCATC	6 cut(s)	86, 229, 389, 669, 856, 1040
MaeI	CTAG	4 cut(s)	182, 512, 531, 1385
MaeII	ACGT	6 cut(s)	143, 786, 971, 1083, 1096, 1185
MaeIII	GTNAC	5 cut(s)	193, 877, 1109, 1219, 1379
MalI	GATC	5 cut(s)	17, 35, 423, 639, 1211
MbiI	CCGCTC	1 cut(s)	1020
MboI	GATC	5 cut(s)	15, 33, 421, 637, 1209
MboII	GAAGA	9 cut(s)	444, 531, 534, 537, 838, 847, 1033, 1135, 1387
MhlI	GDGCHC	2 cut(s)	246, 1319
MluCI	AATT	6 cut(s)	43, 60, 436, 505, 709, 1564
Mly113I	GGCGCC	1 cut(s)	1026
MlyI	GAGTC	1 cut(s)	1574
MmeI	TCCRAC	3 cut(s)	369, 832, 1419
Mph1103I	ATGCAT	1 cut(s)	561
MseI	TTAA	3 cut(s)	374, 822, 1503
MslI	CAYNNNNRTG	2 cut(s)	29, 785
MspA1I	CMGCKG	1 cut(s)	677
MspR9I	CCNGG	7 cut(s)	617, 883, 1234, 1474, 1512, 1585, 1593
MvaI	CCWGG	2 cut(s)	1512, 1585
MvnI	CGCG	1 cut(s)	156
NarI	GGCGCC	1 cut(s)	1026
NciI	CCSGG	5 cut(s)	617, 883, 1234, 1474, 1593
NcoI	CCATGG	1 cut(s)	198
NdeII	GATC	5 cut(s)	15, 33, 421, 637, 1209
NheI	GCTAGC	1 cut(s)	511
NlaIII	CATG	5 cut(s)	84, 202, 372, 970, 1201
NlaIV	GGNNCC	5 cut(s)	614, 766, 1027, 1129, 1293
NmeAIII	GCCGAG	1 cut(s)	530
NmuCI	GTSAC	3 cut(s)	193, 1109, 1219
NruI	TCGCGA	1 cut(s)	156
NsiI	ATGCAT	1 cut(s)	561
NspV	TTCGAA	1 cut(s)	975
PfeI	GAWTC	1 cut(s)	1588
PflMI	CCANNNNNTGG	1 cut(s)	1009
PfoI	TCCNGGA	3 cut(s)	615, 1232, 1583
PleI	GAGTC	1 cut(s)	1574
PluTI	GGCGCC	1 cut(s)	1029
PpsI	GAGTC	1 cut(s)	1574
Ppu21I	YACGTR	1 cut(s)	787
PpuMI	RGGWCCY	1 cut(s)	1326
PsiI	TTATAA	1 cut(s)	1526
Psp5II	RGGWCCY	1 cut(s)	1326
Psp6I	CCWGG	2 cut(s)	1510, 1583
PspEI	GGTNACC	1 cut(s)	193
PspGI	CCWGG	2 cut(s)	1510, 1583
PspN4I	GGNNCC	5 cut(s)	614, 766, 1027, 1129, 1293
PspPI	GGNCC	7 cut(s)	202, 665, 765, 989, 1225, 1297, 1326
PspPPI	RGGWCCY	1 cut(s)	1326
PstI	CTGCAG	1 cut(s)	742
PvuII	CAGCTG	1 cut(s)	677
RruI	TCGCGA	1 cut(s)	156
RsaI	GTAC	4 cut(s)	57, 260, 535, 576
RsaNI	GTAC	4 cut(s)	56, 259, 534, 575
RseI	CAYNNNNRTG	2 cut(s)	29, 785
SaqAI	TTAA	3 cut(s)	374, 822, 1503
Sau3AI	GATC	5 cut(s)	15, 33, 421, 637, 1209
Sau96I	GGNCC	7 cut(s)	202, 665, 765, 989, 1225, 1297, 1326
ScaI	AGTACT	1 cut(s)	535
SchI	GAGTC	1 cut(s)	1574
ScrFI	CCNGG	7 cut(s)	617, 883, 1234, 1474, 1512, 1585, 1593
SduI	GDGCHC	2 cut(s)	246, 1319
SfaNI	GCATC	6 cut(s)	86, 229, 389, 669, 856, 1040
SfcI	CTRYAG	2 cut(s)	738, 1045
SfoI	GGCGCC	1 cut(s)	1027
SfuI	TTCGAA	1 cut(s)	975
SinI	GGWCC	6 cut(s)	202, 665, 765, 989, 1297, 1326
SmiMI	CAYNNNNRTG	2 cut(s)	29, 785
SmlI	CTYRAG	5 cut(s)	102, 188, 596, 693, 960
SmoI	CTYRAG	5 cut(s)	102, 188, 596, 693, 960
Sse9I	AATT	6 cut(s)	43, 60, 436, 505, 709, 1564
SsiI	CCGC	7 cut(s)	1018, 1158, 1279, 1289, 1535, 1538, 1544
SspDI	GGCGCC	1 cut(s)	1025
SspMI	CTAG	4 cut(s)	182, 512, 531, 1385
StyD4I	CCNGG	7 cut(s)	615, 881, 1232, 1472, 1510, 1583, 1591
StyI	CCWWGG	3 cut(s)	198, 744, 1418
TaaI	ACNGT	6 cut(s)	413, 1109, 1132, 1219, 1366, 1558
TaiI	ACGT	6 cut(s)	146, 789, 974, 1086, 1099, 1188
TaqI	TCGA	7 cut(s)	140, 213, 223, 335, 640, 656, 975
TasI	AATT	6 cut(s)	43, 60, 436, 505, 709, 1564
TatI	WGTACW	3 cut(s)	55, 258, 533
TauI	GCSGC	4 cut(s)	1160, 1281, 1292, 1538
TfiI	GAWTC	1 cut(s)	1588
Tru1I	TTAA	3 cut(s)	374, 822, 1503
Tru9I	TTAA	3 cut(s)	374, 822, 1503
TscAI	CASTG	1 cut(s)	246
TseFI	GTSAC	3 cut(s)	193, 1109, 1219
TseI	GCWGC	6 cut(s)	84, 737, 740, 1053, 1154, 1484
Tsp45I	GTSAC	3 cut(s)	193, 1109, 1219
TspDTI	ATGAA	6 cut(s)	222, 848, 943, 1129, 1388, 1481
TspRI	CASTG	1 cut(s)	246
Van91I	CCANNNNNTGG	1 cut(s)	1009
VneI	GTGCAC	1 cut(s)	242
VpaK11BI	GGWCC	6 cut(s)	202, 665, 765, 989, 1297, 1326
XapI	RAATTY	2 cut(s)	43, 1564
XmiI	GTMKAC	1 cut(s)	1134
XspI	CTAG	4 cut(s)	182, 512, 531, 1385
ZrmI	AGTACT	1 cut(s)	535
Zsp2I	ATGCAT	1 cut(s)	561

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.