FvH4_4g35210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014220
Physical Location & Seq
Reverse (-)
32585604 .. 32588415
2812 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35210.t1

Sequence Viewer

Length: 1551 bp
ATGTCTCGCACGCCGTGGATTCTACTGCTCCTGCTCTGCTCTCTAGGACTCGCCTCCGCTCTCAAGGTACCCCTTAGAATCAAGGACGTGCTTCCGGTGCTGCCACGTCAGATATCGTGGCCGGTGCTCAACAATTTCCACAGTGCCGTGGATTTGTTGCCGTCGTTTATCGGGTCGGTGAGTCCCAATAACGACTCGATCCAATGGGAAGGTGCTTGCTTTTCCGGCAACGAGGCCCGGTTAGAGTTCGTCGAAGGTGATCGGGGTTTGGGCGGCGGCGTTCTCTATCTCAGGACTTCAGAGGCACACAGTTGGGCGTGTATGGATCTATATGTGTTTGCAACGCCGTATCGGATAACATGGGATTACTACTTTTCTGCGCGGGAGCATACGTTGAAGTTCGACTCATGGGAAGAGCCTGCAGAGTTGGAATATGTAAAGCAGCATGGAGTGTCTGTGTTTCTCATGCCATCGGGGATGCTGGGGACGATGCTCTCCTTGATTGATGTGTTGCCTCTGTTTTCCAACACTGTTTGGGGTCAAAATGCAAACTTAGCTTTCTTGGAAAAGCATATGGGGGCAACCTTTGAGAAACGTTCTCAGCCTTGGCGTGCAACTATAAATCCTGATGATGTTCATAGTGGTGATTTTCTAGCACTTTCGAAGATCCGGGGTCGATGGGGTGGATTTGAGACATTGGAAAAATGGGTCACGGGAGCATTTGCCGGTCATACAGCAGTGTGCTTGAAGGATGAGATGGGAAAACTGTGGGTTGGTGAATCAGGACACGAGAACGAAAAGGGTGAAGAAGTTATAGCAGTGATTCCATGGGAGGAATGGTGGGAGCTACAACTCAAGGATGGTTCTAATCCACAGATAGCCTTACTTCCCTTGCATCCAGAGCTGCGTGAAAAATTTAACTCTACTGCAGCATGGGAATATGCTCGGAGCATGTCAGGGAAACCATATGGTTATCACAACATGATATTTAGCTGGATTGACACCATGGCTGAAAACTATCCCCCACCTCTTGATACTCATTTGGTTGTTTCTGTCATGTCTATGTGGACTAGGGTGCAGCCGGCTTATGCTGCTAATATGTGGAATGAGGCTCTAAACAAGCGGCTAAGTACTGAGGGATTGGACCTGTATGAGATACTGGCTGAGGTTGAAAATCGCGGGATATCTGTCGATCACTTGCTTACTATTCCAGAACAAGATGAGTGGGTGTACAGTGATGGCAAATCTACAACCTGTGTTGCTTTCATTCTTGCAATGTATAAAGAAGCCGGGATTTTTGGTCCTATTTCGAACTCTATTCAAGTTACTGAATTCACTATTCGTGATGCGTACATGCTAAAGATTTTTGAGAATAACCAGACACGTTTGCCAAGTTGGTGTAACTTAGGGGATGAAAAGCTCCCATTCTGCCAGATCCTTGGTGAATATCGAATGGAACTGCCTGAATATAACACCATAGAGCCATACGCAAACATGAATGAGCACTGCCCCTCCCTACCTCCTACTTATATTAGACCTAGTCAATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

517

Amino Acids

58.64

Weight (kDa)

4.97

Isoelectric Point (pI)

37.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014220)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70160
fragaria_vesca FvH4_4g35210
malus_domestica MD13G1085500.v1.1 MD16G1084800.v1.1
prunus_persica Prupe.1G261300_v2.0.a1
pyrus_communis pycom13g07390 pycom16g07340
rosa_chinensis RchiOBHm_Chr4g0444811
rosa_laevigata RLG00000005800
rosa_multiflora Rmu_sc0000096.1_g000022
rosa_roxburghii Rroxscaffold_5G00385300
rosa_rugosa Rorug04G0355300
rosa_samantha Rh4AG416600 Rh4BG428400 Rh4CG443100 Rh4DG424100
rosa_wichuraiana Rw4G035710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 67
AccB1I GGYRCC 1 cut(s) 67
AccBSI CCGCTC 1 cut(s) 59
AccII CGCG 2 cut(s) 382, 1179
AciI CCGC 6 cut(s) 57, 273, 276, 382, 1123, 1179
AclI AACGTT 1 cut(s) 595
AclWI GGATC 4 cut(s) 193, 333, 661, 1429
AcoI YGGCCR 1 cut(s) 119
AcsI RAATTY 2 cut(s) 914, 1331
AcuI CTGAAG 1 cut(s) 282
AdeI CACNNNGTG 1 cut(s) 15
AfaI GTAC 4 cut(s) 69, 1132, 1232, 1352
AflIII ACRYGT 1 cut(s) 1382
AgsI TTSAA 4 cut(s) 397, 748, 1172, 1322
AjiI CACGTC 2 cut(s) 88, 107
AluBI AGCT 5 cut(s) 557, 847, 904, 993, 1420
AluI AGCT 5 cut(s) 557, 847, 904, 993, 1420
Alw21I GWGCWC 2 cut(s) 129, 1506
Alw26I GTCTC 2 cut(s) 9, 686
AlwI GGATC 4 cut(s) 193, 333, 661, 1429
AoxI GGCC 2 cut(s) 119, 234
ApeKI GCWGC 6 cut(s) 100, 442, 904, 929, 1078, 1091
ApoI RAATTY 2 cut(s) 914, 1331
Asp718I GGTACC 1 cut(s) 67
AspLEI GCGC 1 cut(s) 382
AspS9I GGNCC 3 cut(s) 235, 1144, 1301
AsuC2I CCSGG 3 cut(s) 238, 671, 1291
AsuHPI GGTGA 6 cut(s) 190, 269, 656, 788, 815, 1454
AsuII TTCGAA 2 cut(s) 662, 1310
AvaII GGWCC 2 cut(s) 1144, 1301
BanI GGYRCC 1 cut(s) 67
BauI CACGAG 1 cut(s) 788
Bbv12I GWGCWC 2 cut(s) 129, 1506
BbvCI CCTCAGC 1 cut(s) 1164
BbvI GCAGC 6 cut(s) 87, 454, 891, 941, 1078, 1090
BccI CCATC 5 cut(s) 478, 672, 751, 854, 1232
BceAI ACGGC 3 cut(s) 131, 145, 331
BcnI CCSGG 3 cut(s) 238, 671, 1291
BcoDI GTCTC 2 cut(s) 9, 686
BfaI CTAG 4 cut(s) 44, 653, 1071, 1539
BfmI CTRYAG 2 cut(s) 420, 927
BisI GCNGC 9 cut(s) 101, 274, 277, 443, 905, 930, 1079, 1092, 1124
BlsI GCNGC 9 cut(s) 102, 275, 278, 444, 906, 931, 1080, 1093, 1125
BmcAI AGTACT 1 cut(s) 1132
Bme1390I CCNGG 3 cut(s) 238, 671, 1291
Bme18I GGWCC 2 cut(s) 1144, 1301
BmgBI CACGTC 2 cut(s) 88, 107
BmgT120I GGNCC 3 cut(s) 235, 1144, 1301
BmiI GGNNCC 1 cut(s) 69
BmrFI CCNGG 3 cut(s) 238, 671, 1291
BmsI GCATC 4 cut(s) 468, 480, 904, 1336
Bpu10I CCTNAGC 1 cut(s) 1164
Bpu14I TTCGAA 2 cut(s) 662, 1310
BpuEI CTTGAG 2 cut(s) 47, 839
BpuMI CCSGG 3 cut(s) 238, 671, 1291
BsaJI CCNNGG 7 cut(s) 14, 147, 605, 670, 827, 1005, 1438
BsaWI WCCGGW 1 cut(s) 94
BsaXI ACNNNNNCTCC 8 cut(s) 377, 407, 441, 471, 824, 854, 1496, 1526
Bse118I RCCGGY 3 cut(s) 121, 725, 1081
Bse1I ACTGG 1 cut(s) 1164
Bse3DI GCAATG 1 cut(s) 1281
BseDI CCNNGG 7 cut(s) 14, 147, 605, 670, 827, 1005, 1438
BseGI GGATG 5 cut(s) 483, 757, 865, 895, 1417
BseMI GCAATG 1 cut(s) 1281
BseMII CTCAG 4 cut(s) 304, 614, 1125, 1155
BseNI ACTGG 1 cut(s) 1164
BseXI GCAGC 6 cut(s) 87, 454, 891, 941, 1078, 1090
BseYI CCCAGC 1 cut(s) 481
BsgI GTGCAG 1 cut(s) 1097
Bsh1236I CGCG 2 cut(s) 382, 1179
BshFI GGCC 2 cut(s) 121, 236
BshNI GGYRCC 1 cut(s) 67
BsiHKAI GWGCWC 2 cut(s) 129, 1506
BsiSI CCGG 8 cut(s) 95, 122, 225, 238, 670, 726, 1082, 1290
BslFI GGGAC 2 cut(s) 168, 499
BsmAI GTCTC 2 cut(s) 9, 686
BsmFI GGGAC 2 cut(s) 168, 499
BsnI GGCC 2 cut(s) 121, 236
Bsp119I TTCGAA 2 cut(s) 662, 1310
Bsp1286I GDGCHC 2 cut(s) 129, 1506
Bsp1407I TGTACA 1 cut(s) 1230
Bsp143I GATC 6 cut(s) 198, 259, 325, 666, 1192, 1434
Bsp19I CCATGG 2 cut(s) 827, 1005
BspACI CCGC 6 cut(s) 57, 273, 276, 382, 1123, 1179
BspANI GGCC 2 cut(s) 121, 236
BspCNI CTCAG 4 cut(s) 303, 613, 1126, 1156
BspFNI CGCG 2 cut(s) 382, 1179
BspLI GGNNCC 1 cut(s) 69
BspMAI CTGCAG 2 cut(s) 424, 931
BspPI GGATC 4 cut(s) 193, 333, 661, 1429
BspQI GCTCTTC 1 cut(s) 408
BspT104I TTCGAA 2 cut(s) 662, 1310
BspT107I GGYRCC 1 cut(s) 67
BsrBI CCGCTC 1 cut(s) 59
BsrDI GCAATG 1 cut(s) 1281
BsrFI RCCGGY 3 cut(s) 121, 725, 1081
BsrGI TGTACA 1 cut(s) 1230
BsrI ACTGG 1 cut(s) 1164
BssAI RCCGGY 3 cut(s) 121, 725, 1081
BssECI CCNNGG 7 cut(s) 14, 147, 605, 670, 827, 1005, 1438
BssMI GATC 6 cut(s) 198, 259, 325, 666, 1192, 1434
BssSI CACGAG 1 cut(s) 788
BssT1I CCWWGG 4 cut(s) 605, 827, 1005, 1438
Bst2BI CACGAG 1 cut(s) 788
Bst4CI ACNGT 5 cut(s) 143, 311, 532, 768, 1235
Bst6I CTCTTC 1 cut(s) 408
BstAUI TGTACA 1 cut(s) 1230
BstBI TTCGAA 2 cut(s) 662, 1310
BstC8I GCNNGC 5 cut(s) 11, 217, 420, 612, 1083
BstDEI CTNAG 8 cut(s) 74, 290, 553, 600, 1127, 1134, 1164, 1405
BstDSI CCRYGG 4 cut(s) 14, 147, 827, 1005
BstF5I GGATG 5 cut(s) 483, 757, 865, 895, 1417
BstFNI CGCG 2 cut(s) 382, 1179
BstHHI GCGC 1 cut(s) 382
BstKTI GATC 6 cut(s) 201, 262, 328, 669, 1195, 1437
BstMAI GTCTC 2 cut(s) 9, 686
BstMBI GATC 6 cut(s) 198, 259, 325, 666, 1192, 1434
BstMWI GCNNNNNNNGC 5 cut(s) 97, 225, 554, 901, 1091
BstNSI RCATGY 2 cut(s) 955, 1357
BstSCI CCNGG 3 cut(s) 236, 669, 1289
BstSFI CTRYAG 2 cut(s) 420, 927
BstUI CGCG 2 cut(s) 382, 1179
BstV1I GCAGC 6 cut(s) 87, 454, 891, 941, 1078, 1090
BstX2I RGATCY 3 cut(s) 325, 666, 1434
BstXI CCANNNNNNTGG 1 cut(s) 1439
BstYI RGATCY 3 cut(s) 325, 666, 1434
BsuRI GGCC 2 cut(s) 121, 236
BtgI CCRYGG 4 cut(s) 14, 147, 827, 1005
BtrI CACGTC 2 cut(s) 88, 107
BtsCI GGATG 5 cut(s) 483, 757, 865, 895, 1417
BtsI GCAGTG 3 cut(s) 744, 825, 1504
BtsIMutI CAGTG 6 cut(s) 148, 528, 744, 825, 1240, 1504
Cac8I GCNNGC 5 cut(s) 11, 217, 420, 612, 1083
CfoI GCGC 1 cut(s) 382
Cfr10I RCCGGY 3 cut(s) 121, 725, 1081
Cfr13I GGNCC 3 cut(s) 235, 1144, 1301
Csp6I GTAC 4 cut(s) 68, 1131, 1231, 1351
CspCI CAANNNNNGTGG 2 cut(s) 1205, 1240
CviQI GTAC 4 cut(s) 68, 1131, 1231, 1351
DdeI CTNAG 8 cut(s) 74, 290, 553, 600, 1127, 1134, 1164, 1405
DpnI GATC 6 cut(s) 200, 261, 327, 668, 1194, 1436
DpnII GATC 6 cut(s) 198, 259, 325, 666, 1192, 1434
DraIII CACNNNGTG 1 cut(s) 15
EaeI YGGCCR 1 cut(s) 119
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
Eco130I CCWWGG 4 cut(s) 605, 827, 1005, 1438
Eco32I GATATC 2 cut(s) 114, 1185
Eco47I GGWCC 2 cut(s) 1144, 1301
Eco57I CTGAAG 1 cut(s) 282
EcoRI GAATTC 1 cut(s) 1331
EcoRV GATATC 2 cut(s) 114, 1185
EcoT14I CCWWGG 4 cut(s) 605, 827, 1005, 1438
ErhI CCWWGG 4 cut(s) 605, 827, 1005, 1438
FaqI GGGAC 2 cut(s) 168, 499
FauI CCCGC 2 cut(s) 375, 1172
FauNDI CATATG 2 cut(s) 573, 967
Fnu4HI GCNGC 9 cut(s) 101, 274, 277, 443, 905, 930, 1079, 1092, 1124
FokI GGATG 5 cut(s) 490, 764, 872, 882, 1424
Fsp4HI GCNGC 9 cut(s) 101, 274, 277, 443, 905, 930, 1079, 1092, 1124
FspBI CTAG 4 cut(s) 44, 653, 1071, 1539
GlaI GCGC 1 cut(s) 381
GluI GCNGC 9 cut(s) 101, 274, 277, 443, 905, 930, 1079, 1092, 1124
GsaI CCCAGC 1 cut(s) 485
HaeIII GGCC 2 cut(s) 121, 236
HapII CCGG 8 cut(s) 95, 122, 225, 238, 670, 726, 1082, 1290
HhaI GCGC 1 cut(s) 382
Hin6I GCGC 1 cut(s) 380
HinP1I GCGC 1 cut(s) 380
HinfI GANTC 8 cut(s) 19, 48, 78, 181, 194, 404, 779, 823
HpaII CCGG 8 cut(s) 95, 122, 225, 238, 670, 726, 1082, 1290
HphI GGTGA 6 cut(s) 190, 269, 656, 788, 815, 1454
Hpy166II GTNNAC 2 cut(s) 1068, 1231
Hpy188I TCNGA 4 cut(s) 111, 301, 354, 948
Hpy188III TCNNGA 7 cut(s) 292, 626, 783, 899, 1031, 1211, 1343
Hpy8I GTNNAC 2 cut(s) 1068, 1231
Hpy99I CGWCG 2 cut(s) 166, 254
HpyAV CCTTC 3 cut(s) 203, 248, 742
HpyCH4III ACNGT 5 cut(s) 143, 311, 532, 768, 1235
HpyCH4IV ACGT 5 cut(s) 87, 106, 392, 595, 1384
HpyCH4V TGCA 8 cut(s) 341, 422, 548, 614, 895, 929, 1078, 1274
HpyF10VI GCNNNNNNNGC 5 cut(s) 97, 225, 554, 901, 1091
HpyF3I CTNAG 8 cut(s) 74, 290, 553, 600, 1127, 1134, 1164, 1405
HpySE526I ACGT 5 cut(s) 87, 106, 392, 595, 1384
HspAI GCGC 1 cut(s) 380
KpnI GGTACC 1 cut(s) 71
KroI GCCGGC 1 cut(s) 1081
KroNI GCCGGC 1 cut(s) 1083
Kzo9I GATC 6 cut(s) 198, 259, 325, 666, 1192, 1434
LguI GCTCTTC 1 cut(s) 408
LmnI GCTCC 6 cut(s) 33, 385, 716, 844, 948, 1425
Lsp1109I GCAGC 6 cut(s) 87, 454, 891, 941, 1078, 1090
LweI GCATC 4 cut(s) 468, 480, 904, 1336
MaeI CTAG 4 cut(s) 44, 653, 1071, 1539
MaeII ACGT 5 cut(s) 87, 106, 392, 595, 1384
MaeIII GTNAC 3 cut(s) 709, 1324, 1400
MalI GATC 6 cut(s) 200, 261, 327, 668, 1194, 1436
MbiI CCGCTC 1 cut(s) 59
MboI GATC 6 cut(s) 198, 259, 325, 666, 1192, 1434
MboII GAAGA 3 cut(s) 425, 676, 818
MflI RGATCY 3 cut(s) 325, 666, 1434
MhlI GDGCHC 2 cut(s) 129, 1506
MluCI AATT 3 cut(s) 133, 914, 1331
MlyI GAGTC 4 cut(s) 42, 188, 190, 398
MmeI TCCRAC 2 cut(s) 408, 549
MroNI GCCGGC 1 cut(s) 1081
MseI TTAA 1 cut(s) 918
MslI CAYNNNNRTG 2 cut(s) 642, 1061
MspI CCGG 8 cut(s) 95, 122, 225, 238, 670, 726, 1082, 1290
MspR9I CCNGG 3 cut(s) 238, 671, 1291
MvnI CGCG 2 cut(s) 382, 1179
MwoI GCNNNNNNNGC 5 cut(s) 97, 225, 554, 901, 1091
NaeI GCCGGC 1 cut(s) 1083
NciI CCSGG 3 cut(s) 238, 671, 1291
NcoI CCATGG 2 cut(s) 827, 1005
NdeI CATATG 2 cut(s) 573, 967
NdeII GATC 6 cut(s) 198, 259, 325, 666, 1192, 1434
NgoMIV GCCGGC 1 cut(s) 1081
NlaIV GGNNCC 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 709
NspI RCATGY 2 cut(s) 955, 1357
NspV TTCGAA 2 cut(s) 662, 1310
PciSI GCTCTTC 1 cut(s) 408
PdiI GCCGGC 1 cut(s) 1083
PfeI GAWTC 4 cut(s) 19, 78, 779, 823
PflFI GACNNNGTC 1 cut(s) 1539
PkrI GCNGC 9 cut(s) 102, 275, 278, 444, 906, 931, 1080, 1093, 1125
PleI GAGTC 4 cut(s) 42, 188, 189, 398
PpsI GAGTC 4 cut(s) 42, 188, 189, 398
Psp1406I AACGTT 1 cut(s) 595
PspFI CCCAGC 1 cut(s) 481
PspN4I GGNNCC 1 cut(s) 69
PspPI GGNCC 3 cut(s) 235, 1144, 1301
PstI CTGCAG 2 cut(s) 424, 931
PsuI RGATCY 3 cut(s) 325, 666, 1434
PsyI GACNNNGTC 1 cut(s) 1539
RsaI GTAC 4 cut(s) 69, 1132, 1232, 1352
RsaNI GTAC 4 cut(s) 68, 1131, 1231, 1351
RseI CAYNNNNRTG 2 cut(s) 642, 1061
SapI GCTCTTC 1 cut(s) 408
SaqAI TTAA 1 cut(s) 918
SatI GCNGC 9 cut(s) 101, 274, 277, 443, 905, 930, 1079, 1092, 1124
Sau3AI GATC 6 cut(s) 198, 259, 325, 666, 1192, 1434
Sau96I GGNCC 3 cut(s) 235, 1144, 1301
ScaI AGTACT 1 cut(s) 1132
SchI GAGTC 4 cut(s) 42, 188, 190, 398
ScrFI CCNGG 3 cut(s) 238, 671, 1291
SduI GDGCHC 2 cut(s) 129, 1506
SfaNI GCATC 4 cut(s) 468, 480, 904, 1336
SfcI CTRYAG 2 cut(s) 420, 927
SfuI TTCGAA 2 cut(s) 662, 1310
SinI GGWCC 2 cut(s) 1144, 1301
SmiMI CAYNNNNRTG 2 cut(s) 642, 1061
SmlI CTYRAG 2 cut(s) 62, 854
SmoI CTYRAG 2 cut(s) 62, 854
Sse9I AATT 3 cut(s) 133, 914, 1331
SsiI CCGC 6 cut(s) 57, 273, 276, 382, 1123, 1179
SspMI CTAG 4 cut(s) 44, 653, 1071, 1539
StyD4I CCNGG 3 cut(s) 236, 669, 1289
StyI CCWWGG 4 cut(s) 605, 827, 1005, 1438
TaaI ACNGT 5 cut(s) 143, 311, 532, 768, 1235
TaiI ACGT 5 cut(s) 90, 109, 395, 598, 1387
TaqI TCGA 8 cut(s) 197, 252, 402, 662, 676, 1191, 1310, 1450
TasI AATT 3 cut(s) 133, 914, 1331
TatI WGTACW 2 cut(s) 1130, 1230
TauI GCSGC 3 cut(s) 276, 279, 1126
TfiI GAWTC 4 cut(s) 19, 78, 779, 823
Tru1I TTAA 1 cut(s) 918
Tru9I TTAA 1 cut(s) 918
TscAI CASTG 6 cut(s) 148, 535, 744, 825, 1240, 1511
TseFI GTSAC 1 cut(s) 709
TseI GCWGC 6 cut(s) 100, 442, 904, 929, 1078, 1091
Tsp45I GTSAC 1 cut(s) 709
TspDTI ATGAA 4 cut(s) 626, 1255, 1428, 1511
TspRI CASTG 6 cut(s) 148, 535, 744, 825, 1240, 1511
Tth111I GACNNNGTC 1 cut(s) 1539
VpaK11BI GGWCC 2 cut(s) 1144, 1301
XapI RAATTY 2 cut(s) 914, 1331
XceI RCATGY 2 cut(s) 955, 1357
XcmI CCANNNNNNNNNTGG 1 cut(s) 834
XspI CTAG 4 cut(s) 44, 653, 1071, 1539
ZrmI AGTACT 1 cut(s) 1132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.