Prupe.1G261300_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0014220
Physical Location & Seq
Reverse (-)
27013400 .. 27016890
3491 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G261300.1

Sequence Viewer

Length: 1581 bp
ATGTCTCTCTTCTCTCGCACTCCATGGCTATTACTGCTGCTGTTGTGCTTTCTAGGGTTAGGATTAGGATTTGGGGACGCTCTCAAGGTCCCCCTCAGGATCAAGGATGTGCTTCCGGTTCTGCCACGTCAGATATCGTGGCCGGTGCTCAACAATTTCCACAGTGCTGTGGATTTGTTGCCTTCTTTTATCGGATCGGTGACTGCCGACAATACATCAATTGAATGGGAAGGAGCTTGCTTTTCTGGTAACCAGGCTCGGCTCGAGTTCACCGAAGGCGATCGGGACAAACCCAATTTGGGAGGTGGCGTTCTCTATCTCAAGACTTCTGAGGCACACAGTTGGGCTTGTATGGATCTGTATGTTTTTGCAACACCCTATCGGATTACATGGGATTACTACTTCTCTGCTCGAGAACATACCTTGAAATTCGACTCGTGGGAAGAGCAGGCAGAGTTGGAATATGTAAAGCAGCATGGGGTCTCCGTGTTTCTCATGCCATCAGGGATGCTGGGGACCATGCTTTCTTTAATAGATGTTTTGCCTCTGTTTTCTAACACTGCTTGGGGTCAAAGTGCAAACATTGATTTTTTGAAGAAGCACATGGGTGCTACATTTGAAAAACGTTCTCAGCCTTGGCAGACAACTATAAATCCAGAAGATGTTCATTCTGGTGATTTTCTAGCAGTTTCAAAGATCCGTGGTCGATGGGGTGGATTCGAGACTTTGGAAAAATGGGTAACCGGAGCATTTGCTGGCCATACAGCAGTGTGCTTGAAGGATGAGATGGGCAATCTATGGGTTGGCGAATCAGGACATGAAAATGAAAAGGGTGAAGAAATTATAGCAGTGGTTCCATGGGATGAGTGGTGGGAACTGGCACTCAAGGATAGTTCTAATCCACAAATAGCCTTGCTTCCCTTGCATCCAGAATTGCGTGCAAAATTCAATTCTACGGCAGCATGGGAATATGCTCGGAGTATGTCAGGGAAGCCATATGGTTATCACAATATGATATTTAGCTGGATTGACACCATGGCTGACAACTATCCTCCTCCTCTTGATACTCACTTGGTTGTTTCTGTCATGTCTATGTGGACCAGGATGCAGCCTGCTTATGCTGCAAATATGTGGAATGAAGCTCTCAACAAGCGGCTGGGGACAGAGGGTTTGGACCTGTACGAGATTCTAACTGAGGTTGAGAATCGTGAAATAGCCTTTGATCATTTGCTTACAATTCCAGAACAAGATGAATGGGTATACAGCGATGGCAAATCTACAACATGTGTTGCTTTTATTCTTGCAATGTATAAAGAAGCTGGGATTTTTGGCCCTATTTCCAACTCTATCCAAGTTACTGAATTCACTATTCGTGATGCGTACATGCTTAAGATTTTTGAGGATAACCAAACCCGCTTGCCAAGTTGGTGCAATGGCAAGGACGAAAAGCTCCCATTCTGCCAGATCCTTGGTGAATATCGGATGGAACTGCCTGAATATAACACCATAGAGCCATACACTAACATGAATGAGCACTGCCCCTCCTTACCTCCTACTTACGACAGACCACTACGATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

527

Amino Acids

59.92

Weight (kDa)

4.9

Isoelectric Point (pI)

37.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014220)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70160
fragaria_vesca FvH4_4g35210
malus_domestica MD13G1085500.v1.1 MD16G1084800.v1.1
prunus_persica Prupe.1G261300_v2.0.a1
pyrus_communis pycom13g07390 pycom16g07340
rosa_chinensis RchiOBHm_Chr4g0444811
rosa_laevigata RLG00000005800
rosa_multiflora Rmu_sc0000096.1_g000022
rosa_roxburghii Rroxscaffold_5G00385300
rosa_rugosa Rorug04G0355300
rosa_samantha Rh4AG416600 Rh4BG428400 Rh4CG443100 Rh4DG424100
rosa_wichuraiana Rw4G035710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1260
AciI CCGC 2 cut(s) 1153, 1414
AclI AACGTT 1 cut(s) 625
AclWI GGATC 5 cut(s) 107, 202, 363, 691, 1459
AcoI YGGCCR 2 cut(s) 140, 757
AcsI RAATTY 3 cut(s) 428, 944, 1361
AfaI GTAC 2 cut(s) 1181, 1382
AfiI CCNNNNNNNGG 1 cut(s) 299
AflII CTTAAG 1 cut(s) 1388
AflIII ACRYGT 1 cut(s) 1283
AgsI TTSAA 7 cut(s) 224, 427, 595, 620, 693, 778, 949
AjiI CACGTC 1 cut(s) 128
AjnI CCWGG 2 cut(s) 252, 1100
AleI CACNNNNGTG 1 cut(s) 606
AloI GAACNNNNNNTCC 2 cut(s) 294, 326
AluBI AGCT 5 cut(s) 236, 1023, 1142, 1319, 1450
AluI AGCT 5 cut(s) 236, 1023, 1142, 1319, 1450
Alw21I GWGCWC 2 cut(s) 150, 1536
Alw26I GTCTC 3 cut(s) 9, 487, 716
AlwI GGATC 5 cut(s) 107, 202, 363, 691, 1459
Ama87I CYCGRG 2 cut(s) 263, 411
AoxI GGCC 3 cut(s) 140, 757, 1330
ApeKI GCWGC 5 cut(s) 37, 472, 959, 1108, 1121
ApoI RAATTY 3 cut(s) 428, 944, 1361
ArsI GACNNNNNNTTYG 2 cut(s) 1153, 1185
Asp700I GAANNNNTTC 1 cut(s) 663
AspS9I GGNCC 5 cut(s) 88, 516, 1098, 1174, 1331
AsuHPI GGTGA 5 cut(s) 211, 262, 686, 845, 1484
AvaI CYCGRG 2 cut(s) 263, 411
AvaII GGWCC 4 cut(s) 88, 516, 1098, 1174
AxyI CCTNAGG 1 cut(s) 95
BalI TGGCCA 1 cut(s) 759
BauI CACGAG 1 cut(s) 436
Bbv12I GWGCWC 2 cut(s) 150, 1536
BbvI GCAGC 5 cut(s) 24, 484, 971, 1108, 1120
BccI CCATC 5 cut(s) 508, 702, 781, 1262, 1477
BceAI ACGGC 1 cut(s) 972
BciT130I CCWGG 2 cut(s) 254, 1102
BclI TGATCA 1 cut(s) 1222
BcoDI GTCTC 3 cut(s) 9, 487, 716
BfaI CTAG 2 cut(s) 53, 683
BfrI CTTAAG 1 cut(s) 1388
BisI GCNGC 6 cut(s) 38, 473, 960, 1109, 1122, 1154
BlsI GCNGC 6 cut(s) 39, 474, 961, 1110, 1123, 1155
Bme1390I CCNGG 2 cut(s) 254, 1102
Bme18I GGWCC 4 cut(s) 88, 516, 1098, 1174
BmeT110I CYCGRG 2 cut(s) 263, 411
BmgBI CACGTC 1 cut(s) 128
BmgT120I GGNCC 5 cut(s) 88, 516, 1098, 1174, 1331
BmiI GGNNCC 3 cut(s) 90, 517, 855
BmrFI CCNGG 2 cut(s) 254, 1102
BmsI GCATC 5 cut(s) 498, 934, 1095, 1366, 1565
BpuEI CTTGAG 3 cut(s) 68, 305, 869
BsaI GGTCTC 1 cut(s) 487
BsaJI CCNNGG 6 cut(s) 23, 635, 700, 857, 1035, 1468
BsaWI WCCGGW 2 cut(s) 115, 743
BsaXI ACNNNNNCTCC 4 cut(s) 294, 324, 1526, 1556
Bsc4I CCNNNNNNNGG 1 cut(s) 299
Bse118I RCCGGY 1 cut(s) 142
Bse1I ACTGG 1 cut(s) 882
Bse21I CCTNAGG 1 cut(s) 95
Bse3DI GCAATG 2 cut(s) 1311, 1438
BseBI CCWGG 2 cut(s) 254, 1102
BseDI CCNNGG 6 cut(s) 23, 635, 700, 857, 1035, 1468
BseGI GGATG 7 cut(s) 112, 513, 787, 868, 925, 1110, 1488
BseLI CCNNNNNNNGG 1 cut(s) 299
BseMI GCAATG 2 cut(s) 1311, 1438
BseMII CTCAG 4 cut(s) 109, 321, 644, 1185
BseNI ACTGG 1 cut(s) 882
BseRI GAGGAG 2 cut(s) 1044, 1047
BseXI GCAGC 5 cut(s) 24, 484, 971, 1108, 1120
BseYI CCCAGC 3 cut(s) 511, 1156, 1319
Bsh1285I CGRYCG 1 cut(s) 283
BshFI GGCC 3 cut(s) 142, 759, 1332
BsiEI CGRYCG 1 cut(s) 283
BsiHKAI GWGCWC 2 cut(s) 150, 1536
BsiHKCI CYCGRG 2 cut(s) 263, 411
BsiSI CCGG 3 cut(s) 116, 143, 744
BslFI GGGAC 5 cut(s) 74, 89, 299, 529, 1174
BslI CCNNNNNNNGG 1 cut(s) 299
BsmAI GTCTC 3 cut(s) 9, 487, 716
BsmFI GGGAC 5 cut(s) 74, 89, 299, 529, 1174
BsnI GGCC 3 cut(s) 142, 759, 1332
Bso31I GGTCTC 1 cut(s) 487
BsoBI CYCGRG 2 cut(s) 263, 411
Bsp1286I GDGCHC 2 cut(s) 150, 1536
Bsp143I GATC 7 cut(s) 99, 194, 280, 355, 696, 1222, 1464
Bsp19I CCATGG 3 cut(s) 23, 857, 1035
BspACI CCGC 2 cut(s) 1153, 1414
BspANI GGCC 3 cut(s) 142, 759, 1332
BspCNI CTCAG 4 cut(s) 108, 322, 643, 1186
BspLI GGNNCC 3 cut(s) 90, 517, 855
BspPI GGATC 5 cut(s) 107, 202, 363, 691, 1459
BspQI GCTCTTC 1 cut(s) 438
BspTI CTTAAG 1 cut(s) 1388
BspTNI GGTCTC 1 cut(s) 487
BsrDI GCAATG 2 cut(s) 1311, 1438
BsrFI RCCGGY 1 cut(s) 142
BsrI ACTGG 1 cut(s) 882
BssAI RCCGGY 1 cut(s) 142
BssECI CCNNGG 6 cut(s) 23, 635, 700, 857, 1035, 1468
BssMI GATC 7 cut(s) 99, 194, 280, 355, 696, 1222, 1464
BssNAI GTATAC 1 cut(s) 1261
BssSI CACGAG 1 cut(s) 436
BssT1I CCWWGG 5 cut(s) 23, 635, 857, 1035, 1468
Bst1107I GTATAC 1 cut(s) 1261
Bst2BI CACGAG 1 cut(s) 436
Bst2UI CCWGG 2 cut(s) 254, 1102
Bst4CI ACNGT 2 cut(s) 164, 341
Bst6I CTCTTC 2 cut(s) 14, 438
BstAFI CTTAAG 1 cut(s) 1388
BstC8I GCNNGC 6 cut(s) 238, 450, 757, 939, 1113, 1418
BstDEI CTNAG 4 cut(s) 95, 330, 630, 1194
BstDSI CCRYGG 4 cut(s) 23, 700, 857, 1035
BstEII GGTNACC 2 cut(s) 248, 739
BstF5I GGATG 7 cut(s) 112, 513, 787, 868, 925, 1110, 1488
BstKTI GATC 7 cut(s) 102, 197, 283, 358, 699, 1225, 1467
BstMAI GTCTC 3 cut(s) 9, 487, 716
BstMBI GATC 7 cut(s) 99, 194, 280, 355, 696, 1222, 1464
BstMCI CGRYCG 1 cut(s) 283
BstMWI GCNNNNNNNGC 3 cut(s) 34, 922, 1121
BstNI CCWGG 2 cut(s) 254, 1102
BstNSI RCATGY 2 cut(s) 1287, 1387
BstPI GGTNACC 2 cut(s) 248, 739
BstSCI CCNGG 2 cut(s) 252, 1100
BstV1I GCAGC 5 cut(s) 24, 484, 971, 1108, 1120
BstX2I RGATCY 3 cut(s) 355, 696, 1464
BstXI CCANNNNNNTGG 1 cut(s) 1469
BstYI RGATCY 3 cut(s) 355, 696, 1464
BstZ17I GTATAC 1 cut(s) 1261
Bsu36I CCTNAGG 1 cut(s) 95
BsuRI GGCC 3 cut(s) 142, 759, 1332
BtgI CCRYGG 4 cut(s) 23, 700, 857, 1035
BtgZI GCGATG 1 cut(s) 1281
BtrI CACGTC 1 cut(s) 128
BtsCI GGATG 7 cut(s) 112, 513, 787, 868, 925, 1110, 1488
BtsI GCAGTG 4 cut(s) 558, 774, 855, 1534
BtsIMutI CAGTG 5 cut(s) 169, 558, 774, 855, 1534
Cac8I GCNNGC 6 cut(s) 238, 450, 757, 939, 1113, 1418
Cfr10I RCCGGY 1 cut(s) 142
Cfr13I GGNCC 5 cut(s) 88, 516, 1098, 1174, 1331
CseI GACGC 1 cut(s) 86
Csp6I GTAC 2 cut(s) 1180, 1381
CviQI GTAC 2 cut(s) 1180, 1381
DdeI CTNAG 4 cut(s) 95, 330, 630, 1194
DpnI GATC 7 cut(s) 101, 196, 282, 357, 698, 1224, 1466
DpnII GATC 7 cut(s) 99, 194, 280, 355, 696, 1222, 1464
EaeI YGGCCR 2 cut(s) 140, 757
Eam1104I CTCTTC 2 cut(s) 14, 438
EarI CTCTTC 2 cut(s) 14, 438
Eco130I CCWWGG 5 cut(s) 23, 635, 857, 1035, 1468
Eco31I GGTCTC 1 cut(s) 487
Eco32I GATATC 1 cut(s) 135
Eco47I GGWCC 4 cut(s) 88, 516, 1098, 1174
Eco81I CCTNAGG 1 cut(s) 95
Eco88I CYCGRG 2 cut(s) 263, 411
Eco91I GGTNACC 2 cut(s) 248, 739
EcoO109I RGGNCCY 1 cut(s) 88
EcoO65I GGTNACC 2 cut(s) 248, 739
EcoRI GAATTC 1 cut(s) 1361
EcoRII CCWGG 2 cut(s) 252, 1100
EcoRV GATATC 1 cut(s) 135
EcoT14I CCWWGG 5 cut(s) 23, 635, 857, 1035, 1468
ErhI CCWWGG 5 cut(s) 23, 635, 857, 1035, 1468
FaqI GGGAC 5 cut(s) 74, 89, 299, 529, 1174
FauI CCCGC 1 cut(s) 1421
FauNDI CATATG 1 cut(s) 997
FbaI TGATCA 1 cut(s) 1222
FblI GTMKAC 1 cut(s) 1260
Fnu4HI GCNGC 6 cut(s) 38, 473, 960, 1109, 1122, 1154
FokI GGATG 7 cut(s) 119, 520, 794, 875, 912, 1117, 1495
Fsp4HI GCNGC 6 cut(s) 38, 473, 960, 1109, 1122, 1154
FspBI CTAG 2 cut(s) 53, 683
GluI GCNGC 6 cut(s) 38, 473, 960, 1109, 1122, 1154
GsaI CCCAGC 3 cut(s) 515, 1160, 1323
HaeIII GGCC 3 cut(s) 142, 759, 1332
HapII CCGG 3 cut(s) 116, 143, 744
HgaI GACGC 1 cut(s) 86
HinfI GANTC 5 cut(s) 434, 717, 809, 1186, 1204
HpaII CCGG 3 cut(s) 116, 143, 744
HphI GGTGA 5 cut(s) 211, 262, 686, 845, 1484
Hpy166II GTNNAC 3 cut(s) 270, 1098, 1261
Hpy188I TCNGA 6 cut(s) 132, 194, 331, 384, 978, 1482
Hpy8I GTNNAC 3 cut(s) 270, 1098, 1261
HpyAV CCTTC 4 cut(s) 192, 224, 269, 772
HpyCH4III ACNGT 2 cut(s) 164, 341
HpyCH4IV ACGT 2 cut(s) 127, 625
HpyCH4V TGCA 8 cut(s) 371, 578, 925, 941, 1108, 1124, 1304, 1431
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 922, 1121
HpyF3I CTNAG 4 cut(s) 95, 330, 630, 1194
HpySE526I ACGT 2 cut(s) 127, 625
Ksp22I TGATCA 1 cut(s) 1222
Kzo9I GATC 7 cut(s) 99, 194, 280, 355, 696, 1222, 1464
LguI GCTCTTC 1 cut(s) 438
LmnI GCTCC 3 cut(s) 233, 746, 1455
Lsp1109I GCAGC 5 cut(s) 24, 484, 971, 1108, 1120
LweI GCATC 5 cut(s) 498, 934, 1095, 1366, 1565
MaeI CTAG 2 cut(s) 53, 683
MaeII ACGT 2 cut(s) 127, 625
MaeIII GTNAC 4 cut(s) 199, 248, 739, 1354
MalI GATC 7 cut(s) 101, 196, 282, 357, 698, 1224, 1466
MboI GATC 7 cut(s) 99, 194, 280, 355, 696, 1222, 1464
MboII GAAGA 4 cut(s) 455, 607, 671, 848
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 3 cut(s) 355, 696, 1464
MhlI GDGCHC 2 cut(s) 150, 1536
MlsI TGGCCA 1 cut(s) 759
MluNI TGGCCA 1 cut(s) 759
MlyI GAGTC 1 cut(s) 428
MmeI TCCRAC 2 cut(s) 438, 1365
Mox20I TGGCCA 1 cut(s) 759
MroXI GAANNNNTTC 1 cut(s) 663
MscI TGGCCA 1 cut(s) 759
MseI TTAA 2 cut(s) 530, 1389
MslI CAYNNNNRTG 6 cut(s) 606, 672, 822, 1091, 1523, 1573
Msp20I TGGCCA 1 cut(s) 759
MspCI CTTAAG 1 cut(s) 1388
MspI CCGG 3 cut(s) 116, 143, 744
MspR9I CCNGG 2 cut(s) 254, 1102
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 2 cut(s) 254, 1102
MwoI GCNNNNNNNGC 3 cut(s) 34, 922, 1121
NcoI CCATGG 3 cut(s) 23, 857, 1035
NdeI CATATG 1 cut(s) 997
NdeII GATC 7 cut(s) 99, 194, 280, 355, 696, 1222, 1464
NlaIV GGNNCC 3 cut(s) 90, 517, 855
NmeAIII GCCGAG 1 cut(s) 238
NmuCI GTSAC 1 cut(s) 199
NspI RCATGY 2 cut(s) 1287, 1387
OliI CACNNNNGTG 1 cut(s) 606
PaeR7I CTCGAG 2 cut(s) 263, 411
PciI ACATGT 1 cut(s) 1283
PciSI GCTCTTC 1 cut(s) 438
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PdmI GAANNNNTTC 1 cut(s) 663
PfeI GAWTC 4 cut(s) 717, 809, 1186, 1204
PkrI GCNGC 6 cut(s) 39, 474, 961, 1110, 1123, 1155
Ple19I CGATCG 1 cut(s) 283
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
PpuMI RGGWCCY 1 cut(s) 88
PscI ACATGT 1 cut(s) 1283
Psp1406I AACGTT 1 cut(s) 625
Psp5II RGGWCCY 1 cut(s) 88
Psp6I CCWGG 2 cut(s) 252, 1100
PspEI GGTNACC 2 cut(s) 248, 739
PspFI CCCAGC 3 cut(s) 511, 1156, 1319
PspGI CCWGG 2 cut(s) 252, 1100
PspN4I GGNNCC 3 cut(s) 90, 517, 855
PspPI GGNCC 5 cut(s) 88, 516, 1098, 1174, 1331
PspPPI RGGWCCY 1 cut(s) 88
PspXI VCTCGAGB 1 cut(s) 263
PsuI RGATCY 3 cut(s) 355, 696, 1464
PvuI CGATCG 1 cut(s) 283
RsaI GTAC 2 cut(s) 1181, 1382
RsaNI GTAC 2 cut(s) 1180, 1381
RseI CAYNNNNRTG 6 cut(s) 606, 672, 822, 1091, 1523, 1573
SapI GCTCTTC 1 cut(s) 438
SaqAI TTAA 2 cut(s) 530, 1389
SatI GCNGC 6 cut(s) 38, 473, 960, 1109, 1122, 1154
Sau3AI GATC 7 cut(s) 99, 194, 280, 355, 696, 1222, 1464
Sau96I GGNCC 5 cut(s) 88, 516, 1098, 1174, 1331
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 2 cut(s) 254, 1102
SduI GDGCHC 2 cut(s) 150, 1536
SfaNI GCATC 5 cut(s) 498, 934, 1095, 1366, 1565
Sfr274I CTCGAG 2 cut(s) 263, 411
SinI GGWCC 4 cut(s) 88, 516, 1098, 1174
SlaI CTCGAG 2 cut(s) 263, 411
SmiMI CAYNNNNRTG 6 cut(s) 606, 672, 822, 1091, 1523, 1573
SmlI CTYRAG 6 cut(s) 83, 263, 320, 411, 884, 1388
SmoI CTYRAG 6 cut(s) 83, 263, 320, 411, 884, 1388
SsiI CCGC 2 cut(s) 1153, 1414
SspMI CTAG 2 cut(s) 53, 683
StyD4I CCNGG 2 cut(s) 252, 1100
StyI CCWWGG 5 cut(s) 23, 635, 857, 1035, 1468
TaaI ACNGT 2 cut(s) 164, 341
TaiI ACGT 2 cut(s) 130, 628
TaqI TCGA 5 cut(s) 264, 412, 432, 706, 720
TauI GCSGC 1 cut(s) 1156
TfiI GAWTC 4 cut(s) 717, 809, 1186, 1204
Tru1I TTAA 2 cut(s) 530, 1389
Tru9I TTAA 2 cut(s) 530, 1389
TscAI CASTG 5 cut(s) 169, 565, 774, 855, 1541
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 5 cut(s) 37, 472, 959, 1108, 1121
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 6 cut(s) 656, 834, 840, 1152, 1266, 1541
TspGWI ACGGA 2 cut(s) 475, 689
TspRI CASTG 5 cut(s) 169, 565, 774, 855, 1541
Vha464I CTTAAG 1 cut(s) 1388
VpaK11BI GGWCC 4 cut(s) 88, 516, 1098, 1174
XapI RAATTY 3 cut(s) 428, 944, 1361
XceI RCATGY 2 cut(s) 1287, 1387
XcmI CCANNNNNNNNNTGG 1 cut(s) 864
XhoI CTCGAG 2 cut(s) 263, 411
XmiI GTMKAC 1 cut(s) 1260
XmnI GAANNNNTTC 1 cut(s) 663
XspI CTAG 2 cut(s) 53, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.