Rmu_sc0000096.1_g000022

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000096.1
OG0014220
Physical Location & Seq
Reverse (-)
87179 .. 90216
3038 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000096.1_g000022.1.cds

Sequence Viewer

Length: 1560 bp
atgtctcgcaccccgtggatactattgcttctgctctgctctgtagggtttggggacgctctcaaggtaccccttagaatcaaggacgtccttccggtgctgccacgtcagatatcgtggccggtgctcaacaatttccacagtgccgtggatttgttgccgtcgtttatcgggtcggtgaccccttacaacaactcgattcaatgggaaggagcttgcttttccggcaacgaagcccggctcgagttcaccaaaagcgataggaataaatctgatttgggtggcggcgttctctatctcaagacttctgaggcacacagctgggcgtgtatggatctgtatgtttttgcaacaccgtatcggattacatgggattactacttctcttctcgggagcatacgttgaagttcgactcctgggaagagcctgcagagttggaatatgtaaagcagcatggggtgtctgtgtttctcatgccatcggggatgctggggacaatgctctccttgattgatgtgttgcctctgttttctaacactgtttggggtcaaaatgcaaacttagctttcttggaaaagcacatgggggcaacctttgaaaaacgttctcagccttggcgtgcaactataaatcctgacgatgttcattctggtgattttctagcactttcaaagatccggggtcgatggggtggatttgagacattggaaaaatgggtgactggagcatttgctggtcatacagcagtgtgcttgaaggatgagatgggcaaactatgggttggtgaatcagggcacgagaacgaaaagggtgaagaaattatagcagtggttccatgggaggaatggtgggaattagcactcaaggatggttctaatccacagatagccttacttcctttgcatccagagttgcgtgcaaaatttaactctactgcagcatgggaatatgctcggagcatgtcagggaagccatatggttatcacaacatgatatttagctggattgacaccatggctgaaaactatcccccacctcttgatactcacttggttgtttctgtcatgtctatgtggaccaggttgcagcctgcctatgctgcaaacatgtggaatgaggctctaaacaagcggctaggtactgagggattggacctgtatgagattctagctgaggttgaaaatcgcgagatatctgttgatcacttgcttactattccagaacaagatgagtgggtgtacagtgatggcaaatctacaacctgtgttgctttcattcttgcaatgtataaagaagctgggatttttggtcctatttctaactctattcaagttactgaattcactattcgtgatgcgtacatgctaaagatttttgaggataaccagacccgtttgccaagttggtgtaacgtaggggatgaaaagctcccattctgccagatccttggtgaatatcggatggaactgcctgaatataacaccatagagccatatgcaaacatgaatgagcactgcccctccctacctcctacttatagcagacctagtcgatgctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

519

Amino Acids

59.08

Weight (kDa)

5.06

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014220)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70160
fragaria_vesca FvH4_4g35210
malus_domestica MD13G1085500.v1.1 MD16G1084800.v1.1
prunus_persica Prupe.1G261300_v2.0.a1
pyrus_communis pycom13g07390 pycom16g07340
rosa_chinensis RchiOBHm_Chr4g0444811
rosa_laevigata RLG00000005800
rosa_multiflora Rmu_sc0000096.1_g000022
rosa_roxburghii Rroxscaffold_5G00385300
rosa_rugosa Rorug04G0355300
rosa_samantha Rh4AG416600 Rh4BG428400 Rh4CG443100 Rh4DG424100
rosa_wichuraiana Rw4G035710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 90
Acc65I GGTACC 1 cut(s) 67
AccB1I GGYRCC 1 cut(s) 67
AccII CGCG 1 cut(s) 1188
AciI CCGC 2 cut(s) 285, 1132
AclI AACGTT 1 cut(s) 604
AclWI GGATC 3 cut(s) 342, 670, 1438
AcoI YGGCCR 1 cut(s) 119
AcsI RAATTY 2 cut(s) 923, 1340
AcyI GRCGYC 1 cut(s) 87
AdeI CACNNNGTG 1 cut(s) 15
AfaI GTAC 4 cut(s) 69, 1141, 1241, 1361
AflIII ACRYGT 1 cut(s) 1107
AgsI TTSAA 7 cut(s) 203, 406, 599, 672, 757, 1181, 1331
AjiI CACGTC 1 cut(s) 107
AjnI CCWGG 2 cut(s) 416, 1079
AluBI AGCT 7 cut(s) 215, 321, 566, 1002, 1172, 1298, 1429
AluI AGCT 7 cut(s) 215, 321, 566, 1002, 1172, 1298, 1429
Alw21I GWGCWC 2 cut(s) 129, 1515
Alw26I GTCTC 2 cut(s) 9, 695
AlwI GGATC 3 cut(s) 342, 670, 1438
Ama87I CYCGRG 2 cut(s) 242, 390
AoxI GGCC 1 cut(s) 119
ApeKI GCWGC 5 cut(s) 100, 451, 938, 1087, 1100
ApoI RAATTY 2 cut(s) 923, 1340
Asp718I GGTACC 1 cut(s) 67
AspS9I GGNCC 3 cut(s) 1077, 1153, 1310
AsuC2I CCSGG 2 cut(s) 238, 680
AsuHPI GGTGA 7 cut(s) 190, 241, 665, 730, 797, 824, 1463
AvaI CYCGRG 2 cut(s) 242, 390
AvaII GGWCC 3 cut(s) 1077, 1153, 1310
BaeGI GKGCMC 1 cut(s) 798
BanI GGYRCC 1 cut(s) 67
BauI CACGAG 1 cut(s) 797
Bbv12I GWGCWC 2 cut(s) 129, 1515
BbvCI CCTCAGC 1 cut(s) 1173
BbvI GCAGC 5 cut(s) 87, 463, 950, 1087, 1099
BccI CCATC 6 cut(s) 487, 681, 760, 863, 1241, 1456
BceAI ACGGC 2 cut(s) 131, 145
BciT130I CCWGG 2 cut(s) 418, 1081
BciVI GTATCC 1 cut(s) 12
BclI TGATCA 1 cut(s) 1201
BcnI CCSGG 2 cut(s) 238, 680
BcoDI GTCTC 2 cut(s) 9, 695
BfaI CTAG 4 cut(s) 662, 1136, 1169, 1548
BfmI CTRYAG 3 cut(s) 42, 429, 936
BfuI GTATCC 1 cut(s) 12
BisI GCNGC 7 cut(s) 101, 286, 452, 939, 1088, 1101, 1133
BlsI GCNGC 7 cut(s) 102, 287, 453, 940, 1089, 1102, 1134
Bme1390I CCNGG 4 cut(s) 238, 418, 680, 1081
Bme18I GGWCC 3 cut(s) 1077, 1153, 1310
BmeT110I CYCGRG 2 cut(s) 242, 390
BmgBI CACGTC 1 cut(s) 107
BmgT120I GGNCC 3 cut(s) 1077, 1153, 1310
BmiI GGNNCC 2 cut(s) 69, 834
BmrFI CCNGG 4 cut(s) 238, 418, 680, 1081
BmsI GCATC 4 cut(s) 477, 913, 1345, 1544
BpmI CTGGAG 1 cut(s) 744
Bpu10I CCTNAGC 1 cut(s) 1173
BpuEI CTTGAG 3 cut(s) 47, 284, 848
BpuMI CCSGG 2 cut(s) 238, 680
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 8 cut(s) 14, 147, 417, 614, 679, 836, 1014, 1447
BsaWI WCCGGW 1 cut(s) 94
BsaXI ACNNNNNCTCC 6 cut(s) 386, 416, 833, 863, 1505, 1535
Bse118I RCCGGY 1 cut(s) 121
Bse1I ACTGG 1 cut(s) 727
Bse3DI GCAATG 1 cut(s) 1290
BseBI CCWGG 2 cut(s) 418, 1081
BseDI CCNNGG 8 cut(s) 14, 147, 417, 614, 679, 836, 1014, 1447
BseGI GGATG 6 cut(s) 492, 766, 874, 904, 1426, 1467
BseMI GCAATG 1 cut(s) 1290
BseMII CTCAG 4 cut(s) 300, 623, 1134, 1164
BseNI ACTGG 1 cut(s) 727
BseSI GKGCMC 1 cut(s) 798
BseXI GCAGC 5 cut(s) 87, 463, 950, 1087, 1099
BseYI CCCAGC 3 cut(s) 321, 490, 1298
Bsh1236I CGCG 1 cut(s) 1188
BshFI GGCC 1 cut(s) 121
BshNI GGYRCC 1 cut(s) 67
BsiHKAI GWGCWC 2 cut(s) 129, 1515
BsiHKCI CYCGRG 2 cut(s) 242, 390
BsiSI CCGG 5 cut(s) 95, 122, 225, 238, 679
BslFI GGGAC 2 cut(s) 68, 508
BsmAI GTCTC 2 cut(s) 9, 695
BsmFI GGGAC 2 cut(s) 68, 508
BsnI GGCC 1 cut(s) 121
BsoBI CYCGRG 2 cut(s) 242, 390
Bsp1286I GDGCHC 3 cut(s) 129, 798, 1515
Bsp1407I TGTACA 1 cut(s) 1239
Bsp143I GATC 4 cut(s) 334, 675, 1201, 1443
Bsp19I CCATGG 2 cut(s) 836, 1014
Bsp68I TCGCGA 1 cut(s) 1188
BspACI CCGC 2 cut(s) 285, 1132
BspANI GGCC 1 cut(s) 121
BspCNI CTCAG 4 cut(s) 301, 622, 1135, 1165
BspFNI CGCG 1 cut(s) 1188
BspLI GGNNCC 2 cut(s) 69, 834
BspMAI CTGCAG 2 cut(s) 433, 940
BspPI GGATC 3 cut(s) 342, 670, 1438
BspQI GCTCTTC 1 cut(s) 417
BspT107I GGYRCC 1 cut(s) 67
BsrDI GCAATG 1 cut(s) 1290
BsrFI RCCGGY 1 cut(s) 121
BsrGI TGTACA 1 cut(s) 1239
BsrI ACTGG 1 cut(s) 727
BssAI RCCGGY 1 cut(s) 121
BssECI CCNNGG 8 cut(s) 14, 147, 417, 614, 679, 836, 1014, 1447
BssMI GATC 4 cut(s) 334, 675, 1201, 1443
BssNI GRCGYC 1 cut(s) 87
BssSI CACGAG 1 cut(s) 797
BssT1I CCWWGG 4 cut(s) 614, 836, 1014, 1447
Bst2BI CACGAG 1 cut(s) 797
Bst2UI CCWGG 2 cut(s) 418, 1081
Bst4CI ACNGT 4 cut(s) 143, 357, 541, 1244
Bst6I CTCTTC 2 cut(s) 391, 417
BstACI GRCGYC 1 cut(s) 87
BstAUI TGTACA 1 cut(s) 1239
BstC8I GCNNGC 5 cut(s) 217, 429, 621, 918, 1092
BstDEI CTNAG 6 cut(s) 74, 309, 562, 609, 1143, 1173
BstDSI CCRYGG 4 cut(s) 14, 147, 836, 1014
BstEII GGTNACC 1 cut(s) 178
BstF5I GGATG 6 cut(s) 492, 766, 874, 904, 1426, 1467
BstFNI CGCG 1 cut(s) 1188
BstKTI GATC 4 cut(s) 337, 678, 1204, 1446
BstMAI GTCTC 2 cut(s) 9, 695
BstMBI GATC 4 cut(s) 334, 675, 1201, 1443
BstMWI GCNNNNNNNGC 3 cut(s) 225, 563, 1100
BstNI CCWGG 2 cut(s) 418, 1081
BstNSI RCATGY 3 cut(s) 964, 1111, 1366
BstPI GGTNACC 1 cut(s) 178
BstSCI CCNGG 4 cut(s) 236, 416, 678, 1079
BstSFI CTRYAG 3 cut(s) 42, 429, 936
BstSLI GKGCMC 1 cut(s) 798
BstUI CGCG 1 cut(s) 1188
BstV1I GCAGC 5 cut(s) 87, 463, 950, 1087, 1099
BstX2I RGATCY 3 cut(s) 334, 675, 1443
BstXI CCANNNNNNTGG 1 cut(s) 1448
BstYI RGATCY 3 cut(s) 334, 675, 1443
BsuI GTATCC 1 cut(s) 12
BsuRI GGCC 1 cut(s) 121
BtgI CCRYGG 4 cut(s) 14, 147, 836, 1014
BtrI CACGTC 1 cut(s) 107
BtsCI GGATG 6 cut(s) 492, 766, 874, 904, 1426, 1467
BtsI GCAGTG 3 cut(s) 753, 834, 1513
BtsIMutI CAGTG 6 cut(s) 148, 537, 753, 834, 1249, 1513
BtuMI TCGCGA 1 cut(s) 1188
Cac8I GCNNGC 5 cut(s) 217, 429, 621, 918, 1092
Cfr10I RCCGGY 1 cut(s) 121
Cfr13I GGNCC 3 cut(s) 1077, 1153, 1310
CseI GACGC 1 cut(s) 65
CsiI ACCWGGT 1 cut(s) 1079
Csp6I GTAC 4 cut(s) 68, 1140, 1240, 1360
CspCI CAANNNNNGTGG 2 cut(s) 1214, 1249
CviQI GTAC 4 cut(s) 68, 1140, 1240, 1360
DdeI CTNAG 6 cut(s) 74, 309, 562, 609, 1143, 1173
DpnI GATC 4 cut(s) 336, 677, 1203, 1445
DpnII GATC 4 cut(s) 334, 675, 1201, 1443
DraIII CACNNNGTG 1 cut(s) 15
EaeI YGGCCR 1 cut(s) 119
Eam1104I CTCTTC 2 cut(s) 391, 417
EarI CTCTTC 2 cut(s) 391, 417
Eco130I CCWWGG 4 cut(s) 614, 836, 1014, 1447
Eco32I GATATC 2 cut(s) 114, 1194
Eco47I GGWCC 3 cut(s) 1077, 1153, 1310
Eco88I CYCGRG 2 cut(s) 242, 390
Eco91I GGTNACC 1 cut(s) 178
EcoO65I GGTNACC 1 cut(s) 178
EcoRI GAATTC 1 cut(s) 1340
EcoRII CCWGG 2 cut(s) 416, 1079
EcoRV GATATC 2 cut(s) 114, 1194
EcoT14I CCWWGG 4 cut(s) 614, 836, 1014, 1447
ErhI CCWWGG 4 cut(s) 614, 836, 1014, 1447
FaqI GGGAC 2 cut(s) 68, 508
FauNDI CATATG 2 cut(s) 976, 1495
FbaI TGATCA 1 cut(s) 1201
Fnu4HI GCNGC 7 cut(s) 101, 286, 452, 939, 1088, 1101, 1133
FokI GGATG 6 cut(s) 499, 773, 881, 891, 1433, 1474
Fsp4HI GCNGC 7 cut(s) 101, 286, 452, 939, 1088, 1101, 1133
FspBI CTAG 4 cut(s) 662, 1136, 1169, 1548
GluI GCNGC 7 cut(s) 101, 286, 452, 939, 1088, 1101, 1133
GsaI CCCAGC 3 cut(s) 325, 494, 1302
GsuI CTGGAG 1 cut(s) 744
HaeIII GGCC 1 cut(s) 121
HapII CCGG 5 cut(s) 95, 122, 225, 238, 679
HgaI GACGC 1 cut(s) 65
Hin1I GRCGYC 1 cut(s) 87
HinfI GANTC 5 cut(s) 78, 199, 413, 788, 1165
HpaII CCGG 5 cut(s) 95, 122, 225, 238, 679
HphI GGTGA 7 cut(s) 190, 241, 665, 730, 797, 824, 1463
Hpy166II GTNNAC 3 cut(s) 249, 1077, 1240
Hpy188I TCNGA 6 cut(s) 111, 274, 310, 363, 957, 1461
Hpy188III TCNNGA 8 cut(s) 301, 392, 635, 908, 1040, 1187, 1220, 1352
Hpy8I GTNNAC 3 cut(s) 249, 1077, 1240
Hpy99I CGWCG 1 cut(s) 166
HpyAV CCTTC 3 cut(s) 101, 203, 751
HpyCH4III ACNGT 4 cut(s) 143, 357, 541, 1244
HpyCH4IV ACGT 5 cut(s) 87, 106, 401, 604, 1413
HpyF10VI GCNNNNNNNGC 3 cut(s) 225, 563, 1100
HpyF3I CTNAG 6 cut(s) 74, 309, 562, 609, 1143, 1173
HpySE526I ACGT 5 cut(s) 87, 106, 401, 604, 1413
Hsp92I GRCGYC 1 cut(s) 87
KpnI GGTACC 1 cut(s) 71
Ksp22I TGATCA 1 cut(s) 1201
Kzo9I GATC 4 cut(s) 334, 675, 1201, 1443
LguI GCTCTTC 1 cut(s) 417
LmnI GCTCC 5 cut(s) 212, 394, 725, 957, 1434
Lsp1109I GCAGC 5 cut(s) 87, 463, 950, 1087, 1099
LweI GCATC 4 cut(s) 477, 913, 1345, 1544
MabI ACCWGGT 1 cut(s) 1079
MaeI CTAG 4 cut(s) 662, 1136, 1169, 1548
MaeII ACGT 5 cut(s) 87, 106, 401, 604, 1413
MaeIII GTNAC 4 cut(s) 178, 718, 1333, 1409
MalI GATC 4 cut(s) 336, 677, 1203, 1445
MboI GATC 4 cut(s) 334, 675, 1201, 1443
MboII GAAGA 3 cut(s) 378, 434, 827
MflI RGATCY 3 cut(s) 334, 675, 1443
MhlI GDGCHC 3 cut(s) 129, 798, 1515
MluCI AATT 5 cut(s) 133, 819, 854, 923, 1340
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 1 cut(s) 417
MseI TTAA 1 cut(s) 927
MslI CAYNNNNRTG 2 cut(s) 651, 1070
MspA1I CMGCKG 1 cut(s) 321
MspI CCGG 5 cut(s) 95, 122, 225, 238, 679
MspR9I CCNGG 4 cut(s) 238, 418, 680, 1081
MvaI CCWGG 2 cut(s) 418, 1081
MvnI CGCG 1 cut(s) 1188
MwoI GCNNNNNNNGC 3 cut(s) 225, 563, 1100
NciI CCSGG 2 cut(s) 238, 680
NcoI CCATGG 2 cut(s) 836, 1014
NdeI CATATG 2 cut(s) 976, 1495
NdeII GATC 4 cut(s) 334, 675, 1201, 1443
NlaIV GGNNCC 2 cut(s) 69, 834
NmuCI GTSAC 2 cut(s) 178, 718
NruI TCGCGA 1 cut(s) 1188
NspI RCATGY 3 cut(s) 964, 1111, 1366
PaeR7I CTCGAG 1 cut(s) 242
PciI ACATGT 1 cut(s) 1107
PciSI GCTCTTC 1 cut(s) 417
PfeI GAWTC 4 cut(s) 78, 199, 788, 1165
PflFI GACNNNGTC 1 cut(s) 1548
PkrI GCNGC 7 cut(s) 102, 287, 453, 940, 1089, 1102, 1134
PleI GAGTC 1 cut(s) 407
PpsI GAGTC 1 cut(s) 407
PscI ACATGT 1 cut(s) 1107
Psp1406I AACGTT 1 cut(s) 604
Psp6I CCWGG 2 cut(s) 416, 1079
PspEI GGTNACC 1 cut(s) 178
PspFI CCCAGC 3 cut(s) 321, 490, 1298
PspGI CCWGG 2 cut(s) 416, 1079
PspN4I GGNNCC 2 cut(s) 69, 834
PspPI GGNCC 3 cut(s) 1077, 1153, 1310
PspXI VCTCGAGB 1 cut(s) 242
PstI CTGCAG 2 cut(s) 433, 940
PsuI RGATCY 3 cut(s) 334, 675, 1443
PsyI GACNNNGTC 1 cut(s) 1548
PvuII CAGCTG 1 cut(s) 321
RruI TCGCGA 1 cut(s) 1188
RsaI GTAC 4 cut(s) 69, 1141, 1241, 1361
RsaNI GTAC 4 cut(s) 68, 1140, 1240, 1360
RseI CAYNNNNRTG 2 cut(s) 651, 1070
SapI GCTCTTC 1 cut(s) 417
SaqAI TTAA 1 cut(s) 927
SatI GCNGC 7 cut(s) 101, 286, 452, 939, 1088, 1101, 1133
Sau3AI GATC 4 cut(s) 334, 675, 1201, 1443
Sau96I GGNCC 3 cut(s) 1077, 1153, 1310
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 4 cut(s) 238, 418, 680, 1081
SduI GDGCHC 3 cut(s) 129, 798, 1515
SexAI ACCWGGT 1 cut(s) 1079
SfaNI GCATC 4 cut(s) 477, 913, 1345, 1544
SfcI CTRYAG 3 cut(s) 42, 429, 936
Sfr274I CTCGAG 1 cut(s) 242
SinI GGWCC 3 cut(s) 1077, 1153, 1310
SlaI CTCGAG 1 cut(s) 242
SmiMI CAYNNNNRTG 2 cut(s) 651, 1070
SmlI CTYRAG 4 cut(s) 62, 242, 299, 863
SmoI CTYRAG 4 cut(s) 62, 242, 299, 863
Sse9I AATT 5 cut(s) 133, 819, 854, 923, 1340
SsiI CCGC 2 cut(s) 285, 1132
SspMI CTAG 4 cut(s) 662, 1136, 1169, 1548
StyD4I CCNGG 4 cut(s) 236, 416, 678, 1079
StyI CCWWGG 4 cut(s) 614, 836, 1014, 1447
TaaI ACNGT 4 cut(s) 143, 357, 541, 1244
TaiI ACGT 5 cut(s) 90, 109, 404, 607, 1416
TaqI TCGA 5 cut(s) 197, 243, 411, 685, 1552
TasI AATT 5 cut(s) 133, 819, 854, 923, 1340
TatI WGTACW 1 cut(s) 1239
TauI GCSGC 2 cut(s) 288, 1135
TfiI GAWTC 4 cut(s) 78, 199, 788, 1165
Tru1I TTAA 1 cut(s) 927
Tru9I TTAA 1 cut(s) 927
TscAI CASTG 6 cut(s) 148, 544, 753, 834, 1249, 1520
TseFI GTSAC 2 cut(s) 178, 718
TseI GCWGC 5 cut(s) 100, 451, 938, 1087, 1100
Tsp45I GTSAC 2 cut(s) 178, 718
TspDTI ATGAA 4 cut(s) 635, 1264, 1437, 1520
TspRI CASTG 6 cut(s) 148, 544, 753, 834, 1249, 1520
Tth111I GACNNNGTC 1 cut(s) 1548
VpaK11BI GGWCC 3 cut(s) 1077, 1153, 1310
XapI RAATTY 2 cut(s) 923, 1340
XceI RCATGY 3 cut(s) 964, 1111, 1366
XcmI CCANNNNNNNNNTGG 1 cut(s) 843
XhoI CTCGAG 1 cut(s) 242
XspI CTAG 4 cut(s) 662, 1136, 1169, 1548
ZraI GACGTC 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.