Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0013378

Physical Location & Seq

Genomic Coordinates Forward (+)

32633067 .. 32637756

Gene Structure 4690 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g35290.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3531 bp

ATGGACACACTCTCTATCCTCTCACCCTCTCCCACCTTCTTCTTCACGCGCCTCCTCTCTCTCAAACCCTCACCTCCTCCCTTTCTTCACCACCTCACCCAAACCCTAGCCTTCTCCTCCCCCAAGCCACCACCGCCACCTCTTCCCCCTAATCTACTCCGATTCCGAGTCACTTTCCAGTCCCCTAGCTCCGCCTTCGCCGCGAAATCTCAGCTCTCCGACGCCGACGACGATGAGGAGGAAGACGAGTTCGAGGAGGAGGACGACGACGAAGAAGACGTCGCGGCCGACGAGTACGACGACATTTCCGGCGAAATCTCCGACGACGGCCTCGAACAAAGCGACGATGAAATTGACATGGAGTCGTCTCCGGCGATTCGAAGCGAGGAATTCAGGTGGCAGAGAGTGGAGAAGCTCTGCGAGGACGTCAAGCAGTTCGGCGAGGAAATGATCGACGACGGCGCGCTTGCTTCTATCTACGATTTCCGAATCGATAAGTTTCAGAGATTAGCTATACAAGCATTCTTGAGAGGCTCATCAGTAGTTGTGTCAGCGCCGACGAGTAGCGGAAAGACTTTGATAGCGGAAGCTGCTGCTGTAGCTACTGTAGCTAAAGGAAGGAGATTGTTCTACACAACTCCGCTTAAGGCCTTGTCGAATCAGAAGTTTCGCGAATTTCGTGAAACTTTTGGTGAGGACAATGTTGGCCTTCTCACGGGGGATTCGGCAATAAACAAAGAGGCTCAGGTTCTGATCATGACCACAGAGATTTTGCGCAACATGTTGTATCAGAGTGTTGGAATGGCTTCTGCGCGGGACTCACTATTTCACGTGGATGTAATAGTCTTGGATGAAGTTCATTATCTAAGTGACATATCTCGCGGTACAGTGTGGGAAGAAATTGTTATTTATTCCCCAAAAGAGGTTCAACTCATATGTTTGTCCGCAACAGTTGCTAATCCAGATGAATTGGCTGGTTGGATTGGTCAGATTCATGGTAAAACTGAATTGGTAACTTCAACAAAGCGTCCAGTACCACTGACTTGGCATTTCTCGATGAAGACTTCTTTATTACCACTTCTTGATAAATCTGGAAAACACATGAATAGGAGGTTGTCAGTCAATTATCTGCAACTCAGTGCTCCTGCACCAAAATCTAATAAAGATGATGGATCGAGAAGGAGGAATTCAAGAAGACGGACTAGTGAGACTAGCTATGATGACAGTACTGGTAACATGTCTAGACGACCACTTTCAAAGAATGACATCAACTTAATATACCGTTCACAAGTGCCTCAAGTTACTGATACATTATGGCACCTTAAGGCTAGGGATATGCTACCAGCAGTATGGTTTATTTTTAGCAGGAAAGGTTGTGATGCCGCCGTTCAGTATGTTCAAGATTGCAACCTTTTGGATGACTGTGAAGCAAGTGAGGTTGAATTAGCGTTGAAGAGGTTCCGCCTAAAGTATCCGGATGCTATCAGGGAGAGTTCAGTAAAGGGGTTACTGCGAGGGGTTGCTGCCCATCATGCGGGCTGTCTACCTTTATGGAAATCATTCATAGAAGAGCTGTTTCAGCGTGGTCTTGTTAAGGTTGTATTTGCTACAGAAACACTTGCTGCGGGAATCAATATGCCTGCTAGAACAGCCATTATTGCATCCCTCAGCAAAAGGAGTGACAGTGGACGTACCCTATTGAGCTCAAATGAACTACTTCAAATGGCTGGGCGTGCTGGACGTAGAGGCACGGATGACAGGGGTCATGTAGTGCTTATTCAAAATCCTTACGAGGGTGCTGAAGCTGGTTGCAAGATTCTTTTTGCCGGACTTGAACCTTTGGTATCACAGTTTACTGCTTCATATGGAATGGTGCTAAATCTTCTTGCCGGTTCAAAAGTTACTCGTAGGTCAAATGAATCAGATGAAACAAAAGCCTCCCAATCTGGGCGAACTTTGGACGAAGCTAGAAAGCTAGTTGAACAAAGTTTTGGAAACTATGTTGGGAGCAATGTTATGCTTGCTGCAAAAGAGGAGATTGCTAGAATCGAGAAAGAAATAGAGATGCTGACTTTGGAAATTAGTGATGATGCCATTGACAGAAAAAGCAGGAAGCTCTTGTCTGGACCAGCATACAAGGAGATTGCGAATCTACAGGAAGAATTAAGGGCAGAGAAACGCCTAAGGACTGAACTTCGAAAAAGAATGGAATCTCAGAAGCTTTCTTCACTACGGCCCTTATTAGAGGAATGCGAAAATGGGCAGTTACCCTTTTTGTGCTTGCAGTATAAAGATTCAGAAGGAGTTCAACACTCTATCCCAGCTGTTTATTTGGGGAAGGTTGAGTCACTAAGTGGCTCAAAACTTAAGAACATGGTTTCTGTGGATGACTCTTTTGCACTAACCCCAGTAGCAGTAGAGTCTGAACCAACTTCAGTTTTTGAACCATCGTATTATGCCGCTCTTGGCTCAGATAACTCTTGGTACCTATTTACAGAAAAGTGGATCAAAACAATTTATAAGACAGGCTTTCCAAATGTTGCCTTAGCTCTAGGAGATGCTTTGCCTCGGGAAATAATGAGCATGCTTCTTGATAGAACCGAAATGAAGTGGGAGAAGCTTGCCGAGTCTGATCTTGGTGGTTTTTGGGGCATGGAAGGATCTCTTGAGACGTGGTCTTGGAGTTTGAATGTACCTGTCTTGAATAGTCTTTCTGAACATGATGAGCTTTTACACAAGTCACAAGCATATAATCATGCTGTAGAGCGTTACAAAGAACAAAGAAGTAAAGTTTCACGTCTGAAGAAAAAGATATCACGCACACAAGGCTTCAGAGAATACAAGAAGATCGTAGACATGGCCAGCTTTACTGAGGAGAAGATTAAGCGCTTGAAGGGTAGAGCAAGACGTCTGACTAATCGAATAGAGCAGATTGAACCATCTGGCTGGAAAGAGTTCTTGCAGATCAGCAATGTCATACACGAAACTCGGGCATTAGATATTAACACTCATACCATATTTCCACTTGGTGAAACTGCAGCTGCCATCCGAGGAGAGAATGAACTGTGGCTTGCCATGGTTCTTCGAAATAAGATCTTATTAAGTTTAAAGCCTACAGAACTTGCTGCCGTGTGCGCAAGTTTAGTATCTGAAGGGATCAAAATTCGACCTTGGAAAAATAACAGCTACATATATGAACCTTCAAGCACAGTAGTCGACGTGGTGAGCTTCTTGGACGAGCAAAGAAGCAGTTTCTTACAGCTCCAAGAAAAGCATGGGGTAAATATACCATGCTATTTGGATACCCAGTTTGCAGGCATGGTTGAAGCCTGGGTTTCTGGGTTGACATGGAGGGAGATCATGATGGATTGTGCAATGGACGAGGGCGATTTAGCCCGCCTTCTGCGGCGAACTATTGATCTGCTTGTGCAAATTCCCAAGTTGCCTGATATAGACCCCGTCTTGCAGAGCAATGCAAAGACGGCGTCGAATATCATGGACCGCCCTCCGATTAGTGAGTTGGCTGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000373 GO:0000375 GO:0000377 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006810 GO:0007275 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009892 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010494 GO:0010496 GO:0010497 GO:0010501 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035770 GO:0036464 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0051179 GO:0051234 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C

Protein Analysis

1177

Amino Acids

132.08

Weight (kDa)

5.5

Isoelectric Point (pI)

53.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	166 - 322	6.1e-28	DEAD/DEAH box helicase
ResIII	PF04851	168 - 318	2.1e-10	Type III restriction enzyme, res subunit
DEXQc_Suv3	PF22527	184 - 313	3.7e-08	DEXQ-box helicase domain of Suv3
SH3_ISE2	PF25446	740 - 918	1.6e-86	ISE2, SH3 domain
DSHCT	PF08148	1005 - 1171	8e-34	DSHCT (NUC185) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013378)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70070
fragaria_vesca	FvH4_4g35290 FvH4_4g35290 FvH4_4g35290
malus_domestica	MD16G1083800.v1.1
prunus_persica	Prupe.1G262200_v2.0.a1
pyrus_communis	pycom16g07280
rosa_chinensis	RchiOBHm_Chr4g0444891
rosa_laevigata	RLG00000005791
rosa_multiflora	Rmu_sc0006845.1_g000003 Rmu_sc0013783.1_g000003
rosa_roxburghii	Rroxscaffold_5G00385390
rosa_rugosa	Rorug04G0355800
rosa_samantha	Rh4AG417600 Rh4BG429400 Rh4CG444100 Rh4DG425300
rosa_wichuraiana	Rw4G035790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2520
AatII	GACGTC	3 cut(s)	282, 429, 2911
Acc16I	TGCGCA	2 cut(s)	776, 3136
Acc65I	GGTACC	1 cut(s)	2484
AccB1I	GGYRCC	2 cut(s)	1317, 2484
AccBSI	CCGCTC	1 cut(s)	2462
AccI	GTMKAC	3 cut(s)	1543, 2853, 3216
AccII	CGCG	7 cut(s)	49, 203, 284, 464, 672, 814, 882
AccIII	TCCGGA	1 cut(s)	1474
AclWI	GGATC	4 cut(s)	1180, 2513, 2668, 3164
AcoI	YGGCCR	2 cut(s)	285, 2859
AcsI	RAATTY	5 cut(s)	389, 674, 1186, 3162, 3432
AcuI	CTGAAG	5 cut(s)	1821, 2418, 2815, 2822, 3171
AcvI	CACGTG	1 cut(s)	832
AcyI	GRCGYC	5 cut(s)	222, 279, 426, 2908, 3485
AdeI	CACNNNGTG	2 cut(s)	2354, 3029
AfaI	GTAC	7 cut(s)	296, 886, 1035, 1228, 1693, 2486, 2694
AfeI	AGCGCT	1 cut(s)	2888
AfiI	CCNNNNNNNGG	5 cut(s)	715, 922, 1534, 1793, 1947
AflII	CTTAAG	3 cut(s)	644, 1322, 2366
AflIII	ACRYGT	2 cut(s)	780, 1236
AhdI	GACNNNNNGTC	1 cut(s)	361
AhlI	ACTAGT	1 cut(s)	1202
AjiI	CACGTC	3 cut(s)	2673, 2798, 3220
AjnI	CCWGG	1 cut(s)	3329
AjuI	GAANNNNNNNTTGG	2 cut(s)	1974, 2006
AloI	GAACNNNNNNTCC	2 cut(s)	233, 265
Alw21I	GWGCWC	2 cut(s)	1144, 1706
Alw26I	GTCTC	3 cut(s)	372, 1202, 2663
AlwI	GGATC	4 cut(s)	1180, 2513, 2668, 3164
AlwNI	CAGNNNCTG	1 cut(s)	751
Ama87I	CYCGRG	2 cut(s)	2568, 2988
Aor13HI	TCCGGA	1 cut(s)	1474
Aor51HI	AGCGCT	1 cut(s)	2888
AoxI	GGCC	6 cut(s)	285, 328, 648, 706, 2234, 2859
ApeKI	GCWGC	8 cut(s)	590, 593, 1523, 1622, 2024, 3038, 3041, 3125
ApoI	RAATTY	5 cut(s)	389, 674, 1186, 3162, 3432
ArsI	GACNNNNNNTTYG	4 cut(s)	264, 296, 3470, 3502
Asp700I	GAANNNNTTC	6 cut(s)	805, 1457, 1559, 1716, 2304, 2954
Asp718I	GGTACC	1 cut(s)	2484
AspLEI	GCGC	8 cut(s)	51, 464, 466, 556, 777, 814, 2889, 3137
AspS9I	GGNCC	3 cut(s)	2126, 2235, 3499
AsuHPI	GGTGA	7 cut(s)	15, 63, 80, 88, 704, 3041, 3235
AsuII	TTCGAA	3 cut(s)	379, 2197, 3085
AvaI	CYCGRG	2 cut(s)	2568, 2988
AvaII	GGWCC	2 cut(s)	2126, 3499
AxyI	CCTNAGG	1 cut(s)	2183
BalI	TGGCCA	1 cut(s)	2861
BanI	GGYRCC	2 cut(s)	1317, 2484
BanII	GRGCYC	1 cut(s)	1706
BbrPI	CACGTG	1 cut(s)	832
BbsI	GAAGAC	4 cut(s)	249, 282, 1067, 1201
Bbv12I	GWGCWC	2 cut(s)	1144, 1706
BbvCI	CCTCAGC	1 cut(s)	1667
BbvI	GCAGC	8 cut(s)	577, 580, 1510, 1609, 2011, 3028, 3050, 3112
BccI	CCATC	6 cut(s)	1163, 1536, 2455, 2947, 3053, 3358
BceAI	ACGGC	6 cut(s)	343, 475, 1370, 2249, 3113, 3498
BcgI	CGANNNNNNTGC	2 cut(s)	3196, 3230
BciT130I	CCWGG	1 cut(s)	3331
BciVI	GTATCC	2 cut(s)	1482, 3295
BclI	TGATCA	1 cut(s)	753
BcoDI	GTCTC	3 cut(s)	372, 1202, 2663
BcuI	ACTAGT	1 cut(s)	1202
BfmI	CTRYAG	7 cut(s)	597, 606, 1608, 2153, 2760, 3036, 3114
BfoI	RGCGCY	2 cut(s)	557, 2890
BfrI	CTTAAG	3 cut(s)	644, 1322, 2366
BfuI	GTATCC	2 cut(s)	1482, 3295
BglII	AGATCT	1 cut(s)	3093
BmcAI	AGTACT	1 cut(s)	1228
Bme1390I	CCNGG	1 cut(s)	3331
Bme18I	GGWCC	2 cut(s)	2126, 3499
BmeRI	GACNNNNNGTC	1 cut(s)	361
BmeT110I	CYCGRG	2 cut(s)	2568, 2988
BmgBI	CACGTC	3 cut(s)	2673, 2798, 3220
BmgT120I	GGNCC	3 cut(s)	2126, 2235, 3499
BmiI	GGNNCC	3 cut(s)	1319, 1460, 2486
BmrFI	CCNGG	1 cut(s)	3331
BmrI	ACTGGG	2 cut(s)	2402, 3301
BmsI	GCATC	6 cut(s)	1369, 1468, 1670, 2055, 2080, 2548
BmuI	ACTGGG	2 cut(s)	2402, 3301
BoxI	GACNNNNGTC	1 cut(s)	1761
BpiI	GAAGAC	4 cut(s)	249, 282, 1067, 1201
Bpu10I	CCTNAGC	3 cut(s)	744, 1667, 2545
Bpu14I	TTCGAA	3 cut(s)	379, 2197, 3085
BpuEI	CTTGAG	3 cut(s)	547, 1281, 2687
Bsa29I	ATCGAT	1 cut(s)	492
BsaAI	YACGTR	1 cut(s)	832
BsaHI	GRCGYC	5 cut(s)	222, 279, 426, 2908, 3485
BsaJI	CCNNGG	5 cut(s)	2567, 3049, 3075, 3170, 3330
BsaWI	WCCGGW	1 cut(s)	1474
BsaXI	ACNNNNNCTCC	2 cut(s)	1669, 1699
Bsc4I	CCNNNNNNNGG	5 cut(s)	715, 922, 1534, 1793, 1947
Bse118I	RCCGGY	1 cut(s)	1889
Bse1I	ACTGG	5 cut(s)	178, 1031, 1234, 2408, 3307
Bse21I	CCTNAGG	1 cut(s)	2183
Bse3DI	GCAATG	4 cut(s)	2017, 2977, 3381, 3478
BseAI	TCCGGA	1 cut(s)	1474
BseBI	CCWGG	1 cut(s)	3331
BseCI	ATCGAT	1 cut(s)	492
BseDI	CCNNGG	5 cut(s)	2567, 3049, 3075, 3170, 3330
BseGI	GGATG	8 cut(s)	841, 856, 1423, 1483, 1661, 1759, 2392, 3045
BseLI	CCNNNNNNNGG	5 cut(s)	715, 922, 1534, 1793, 1947
BseMI	GCAATG	4 cut(s)	2017, 2977, 3381, 3478
BseMII	CTCAG	7 cut(s)	224, 758, 1150, 1681, 2228, 2484, 2862
BseNI	ACTGG	5 cut(s)	178, 1031, 1234, 2408, 3307
BsePI	GCGCGC	1 cut(s)	462
BseRI	GAGGAG	9 cut(s)	44, 66, 106, 251, 269, 272, 2048, 2888, 3066
BseX3I	CGGCCG	1 cut(s)	285
BseXI	GCAGC	8 cut(s)	577, 580, 1510, 1609, 2011, 3028, 3050, 3112
BseYI	CCCAGC	2 cut(s)	1727, 2320
BsgI	GTGCAG	1 cut(s)	1131
Bsh1236I	CGCG	7 cut(s)	49, 203, 284, 464, 672, 814, 882
Bsh1285I	CGRYCG	1 cut(s)	288
BshFI	GGCC	6 cut(s)	287, 330, 650, 708, 2236, 2861
BshNI	GGYRCC	2 cut(s)	1317, 2484
BshVI	ATCGAT	1 cut(s)	492
BsiEI	CGRYCG	1 cut(s)	288
BsiHKAI	GWGCWC	2 cut(s)	1144, 1706
BsiHKCI	CYCGRG	2 cut(s)	2568, 2988
BsiSI	CCGG	5 cut(s)	309, 371, 1475, 1827, 1890
BslFI	GGGAC	2 cut(s)	166, 830
BslI	CCNNNNNNNGG	5 cut(s)	715, 922, 1534, 1793, 1947
BsmAI	GTCTC	3 cut(s)	372, 1202, 2663
BsmBI	CGTCTC	2 cut(s)	372, 2663
BsmFI	GGGAC	2 cut(s)	166, 830
BsmI	GAATGC	2 cut(s)	521, 2255
BsnI	GGCC	6 cut(s)	287, 330, 650, 708, 2236, 2861
BsoBI	CYCGRG	2 cut(s)	2568, 2988
Bsp119I	TTCGAA	3 cut(s)	379, 2197, 3085
Bsp1286I	GDGCHC	2 cut(s)	1144, 1706
Bsp13I	TCCGGA	1 cut(s)	1474
Bsp19I	CCATGG	1 cut(s)	3075
Bsp68I	TCGCGA	1 cut(s)	672
BspANI	GGCC	6 cut(s)	287, 330, 650, 708, 2236, 2861
BspCNI	CTCAG	7 cut(s)	223, 757, 1149, 1680, 2227, 2483, 2863
BspDI	ATCGAT	1 cut(s)	492
BspEI	TCCGGA	1 cut(s)	1474
BspFNI	CGCG	7 cut(s)	49, 203, 284, 464, 672, 814, 882
BspHI	TCATGA	2 cut(s)	756, 3360
BspLI	GGNNCC	3 cut(s)	1319, 1460, 2486
BspMAI	CTGCAG	1 cut(s)	3040
BspPI	GGATC	4 cut(s)	1180, 2513, 2668, 3164
BspQI	GCTCTTC	1 cut(s)	1563
BspT104I	TTCGAA	3 cut(s)	379, 2197, 3085
BspT107I	GGYRCC	2 cut(s)	1317, 2484
BspTI	CTTAAG	3 cut(s)	644, 1322, 2366
BsrBI	CCGCTC	1 cut(s)	2462
BsrDI	GCAATG	4 cut(s)	2017, 2977, 3381, 3478
BsrFI	RCCGGY	1 cut(s)	1889
BsrI	ACTGG	5 cut(s)	178, 1031, 1234, 2408, 3307
BssAI	RCCGGY	1 cut(s)	1889
BssECI	CCNNGG	5 cut(s)	2567, 3049, 3075, 3170, 3330
BssHII	GCGCGC	1 cut(s)	462
BssNI	GRCGYC	5 cut(s)	222, 279, 426, 2908, 3485
BssT1I	CCWWGG	2 cut(s)	3075, 3170
Bst2UI	CCWGG	1 cut(s)	3331
Bst6I	CTCTTC	3 cut(s)	147, 1448, 1563
BstACI	GRCGYC	5 cut(s)	222, 279, 426, 2908, 3485
BstAFI	CTTAAG	3 cut(s)	644, 1322, 2366
BstAPI	GCANNNNNTGC	1 cut(s)	953
BstBAI	YACGTR	1 cut(s)	832
BstBI	TTCGAA	3 cut(s)	379, 2197, 3085
BstDSI	CCRYGG	1 cut(s)	3075
BstF5I	GGATG	8 cut(s)	841, 856, 1423, 1483, 1661, 1759, 2392, 3045
BstFNI	CGCG	7 cut(s)	49, 203, 284, 464, 672, 814, 882
BstH2I	RGCGCY	2 cut(s)	557, 2890
BstHHI	GCGC	8 cut(s)	51, 464, 466, 556, 777, 814, 2889, 3137
BstMAI	GTCTC	3 cut(s)	372, 1202, 2663
BstMCI	CGRYCG	1 cut(s)	288
BstNI	CCWGG	1 cut(s)	3331
BstNSI	RCATGY	3 cut(s)	784, 1240, 2587
BstPAI	GACNNNNGTC	1 cut(s)	1761
BstSCI	CCNGG	1 cut(s)	3329
BstSFI	CTRYAG	7 cut(s)	597, 606, 1608, 2153, 2760, 3036, 3114
BstUI	CGCG	7 cut(s)	49, 203, 284, 464, 672, 814, 882
BstV1I	GCAGC	8 cut(s)	577, 580, 1510, 1609, 2011, 3028, 3050, 3112
BstV2I	GAAGAC	4 cut(s)	249, 282, 1067, 1201
BstX2I	RGATCY	2 cut(s)	2660, 3093
BstXI	CCANNNNNNTGG	3 cut(s)	1044, 1350, 2946
BstYI	RGATCY	2 cut(s)	2660, 3093
BstZI	CGGCCG	1 cut(s)	285
Bsu15I	ATCGAT	1 cut(s)	492
Bsu36I	CCTNAGG	1 cut(s)	2183
BsuI	GTATCC	2 cut(s)	1482, 3295
BsuRI	GGCC	6 cut(s)	287, 330, 650, 708, 2236, 2861
BsuTUI	ATCGAT	1 cut(s)	492
BtgI	CCRYGG	1 cut(s)	3075
BtrI	CACGTC	3 cut(s)	2673, 2798, 3220
BtsCI	GGATG	8 cut(s)	841, 856, 1423, 1483, 1661, 1759, 2392, 3045
BtsIMutI	CAGTG	4 cut(s)	894, 1037, 1144, 1690
BtuMI	TCGCGA	1 cut(s)	672
CaiI	CAGNNNCTG	1 cut(s)	751
CciI	TCATGA	2 cut(s)	756, 3360
CfoI	GCGC	8 cut(s)	51, 464, 466, 556, 777, 814, 2889, 3137
Cfr10I	RCCGGY	1 cut(s)	1889
Cfr13I	GGNCC	3 cut(s)	2126, 2235, 3499
ClaI	ATCGAT	1 cut(s)	492
CseI	GACGC	3 cut(s)	230, 1016, 3474
Csp6I	GTAC	7 cut(s)	295, 885, 1034, 1227, 1692, 2485, 2693
CviQI	GTAC	7 cut(s)	295, 885, 1034, 1227, 1692, 2485, 2693
DraI	TTTAAA	1 cut(s)	3108
DraIII	CACNNNGTG	2 cut(s)	2354, 3029
DriI	GACNNNNNGTC	1 cut(s)	361
EaeI	YGGCCR	2 cut(s)	285, 2859
EagI	CGGCCG	1 cut(s)	285
Eam1104I	CTCTTC	3 cut(s)	147, 1448, 1563
Eam1105I	GACNNNNNGTC	1 cut(s)	361
EarI	CTCTTC	3 cut(s)	147, 1448, 1563
EciI	GGCGGA	2 cut(s)	181, 1451
Ecl136II	GAGCTC	1 cut(s)	1704
EclXI	CGGCCG	1 cut(s)	285
Eco130I	CCWWGG	2 cut(s)	3075, 3170
Eco147I	AGGCCT	1 cut(s)	650
Eco24I	GRGCYC	1 cut(s)	1706
Eco32I	GATATC	1 cut(s)	2814
Eco47I	GGWCC	2 cut(s)	2126, 3499
Eco47III	AGCGCT	1 cut(s)	2888
Eco52I	CGGCCG	1 cut(s)	285
Eco53kI	GAGCTC	1 cut(s)	1704
Eco57I	CTGAAG	5 cut(s)	1821, 2418, 2815, 2822, 3171
Eco72I	CACGTG	1 cut(s)	832
Eco81I	CCTNAGG	1 cut(s)	2183
Eco88I	CYCGRG	2 cut(s)	2568, 2988
EcoICRI	GAGCTC	1 cut(s)	1704
EcoRI	GAATTC	2 cut(s)	389, 1186
EcoRII	CCWGG	1 cut(s)	3329
EcoRV	GATATC	1 cut(s)	2814
EcoT14I	CCWWGG	2 cut(s)	3075, 3170
EcoT38I	GRGCYC	1 cut(s)	1706
ErhI	CCWWGG	2 cut(s)	3075, 3170
Esp3I	CGTCTC	2 cut(s)	372, 2663
FalI	AAGNNNNNCTT	4 cut(s)	2513, 2545, 2649, 2681
FaqI	GGGAC	2 cut(s)	166, 830
FauI	CCCGC	4 cut(s)	807, 1528, 1618, 3404
FauNDI	CATATG	2 cut(s)	935, 1864
FbaI	TGATCA	1 cut(s)	753
FblI	GTMKAC	3 cut(s)	1543, 2853, 3216
FokI	GGATG	8 cut(s)	848, 863, 1430, 1490, 1648, 1766, 2399, 3032
FriOI	GRGCYC	1 cut(s)	1706
FspI	TGCGCA	2 cut(s)	776, 3136
GlaI	GCGC	8 cut(s)	50, 463, 465, 555, 776, 813, 2888, 3136
GsaI	CCCAGC	2 cut(s)	1731, 2324
HaeII	RGCGCY	2 cut(s)	557, 2890
HaeIII	GGCC	6 cut(s)	287, 330, 650, 708, 2236, 2861
HapII	CCGG	5 cut(s)	309, 371, 1475, 1827, 1890
HgaI	GACGC	3 cut(s)	230, 1016, 3474
HhaI	GCGC	8 cut(s)	51, 464, 466, 556, 777, 814, 2889, 3137
Hin1I	GRCGYC	5 cut(s)	222, 279, 426, 2908, 3485
Hin6I	GCGC	8 cut(s)	49, 462, 464, 554, 775, 812, 2887, 3135
HinP1I	GCGC	8 cut(s)	49, 462, 464, 554, 775, 812, 2887, 3135
HincII	GTYRAC	2 cut(s)	3217, 3345
HindII	GTYRAC	2 cut(s)	3217, 3345
HindIII	AAGCTT	2 cut(s)	2219, 2618
HpaII	CCGG	5 cut(s)	309, 371, 1475, 1827, 1890
HphI	GGTGA	7 cut(s)	15, 63, 80, 88, 704, 3041, 3235
Hpy166II	GTNNAC	7 cut(s)	1286, 1544, 1688, 1854, 2854, 3217, 3345
Hpy8I	GTNNAC	7 cut(s)	1286, 1544, 1688, 1854, 2854, 3217, 3345
HpyCH4IV	ACGT	9 cut(s)	279, 426, 831, 1690, 1741, 2672, 2797, 2908, 3219
HpySE526I	ACGT	9 cut(s)	279, 426, 831, 1690, 1741, 2672, 2797, 2908, 3219
Hsp92I	GRCGYC	5 cut(s)	222, 279, 426, 2908, 3485
HspAI	GCGC	8 cut(s)	49, 462, 464, 554, 775, 812, 2887, 3135
Kpn2I	TCCGGA	1 cut(s)	1474
KpnI	GGTACC	1 cut(s)	2488
Ksp22I	TGATCA	1 cut(s)	753
LguI	GCTCTTC	1 cut(s)	1563
LmnI	GCTCC	4 cut(s)	194, 1147, 2007, 3267
Lsp1109I	GCAGC	8 cut(s)	577, 580, 1510, 1609, 2011, 3028, 3050, 3112
LweI	GCATC	6 cut(s)	1369, 1468, 1670, 2055, 2080, 2548
MaeII	ACGT	9 cut(s)	279, 426, 831, 1690, 1741, 2672, 2797, 2908, 3219
MbiI	CCGCTC	1 cut(s)	2462
MflI	RGATCY	2 cut(s)	2660, 3093
MhlI	GDGCHC	2 cut(s)	1144, 1706
MlsI	TGGCCA	1 cut(s)	2861
MluNI	TGGCCA	1 cut(s)	2861
MlyI	GAGTC	7 cut(s)	177, 371, 812, 2354, 2384, 2429, 2636
MmeI	TCCRAC	4 cut(s)	243, 345, 778, 959
Mox20I	TGGCCA	1 cut(s)	2861
MroI	TCCGGA	1 cut(s)	1474
MroXI	GAANNNNTTC	6 cut(s)	805, 1457, 1559, 1716, 2304, 2954
MscI	TGGCCA	1 cut(s)	2861
MslI	CAYNNNNRTG	1 cut(s)	834
Msp20I	TGGCCA	1 cut(s)	2861
MspA1I	CMGCKG	2 cut(s)	2324, 3041
MspCI	CTTAAG	3 cut(s)	644, 1322, 2366
MspI	CCGG	5 cut(s)	309, 371, 1475, 1827, 1890
MspR9I	CCNGG	1 cut(s)	3331
Mva1269I	GAATGC	2 cut(s)	521, 2255
MvaI	CCWGG	1 cut(s)	3331
MvnI	CGCG	7 cut(s)	49, 203, 284, 464, 672, 814, 882
NcoI	CCATGG	1 cut(s)	3075
NdeI	CATATG	2 cut(s)	935, 1864
NlaIV	GGNNCC	3 cut(s)	1319, 1460, 2486
NmeAIII	GCCGAG	1 cut(s)	2650
NmuCI	GTSAC	5 cut(s)	169, 869, 1679, 2346, 2739
NruI	TCGCGA	1 cut(s)	672
NsbI	TGCGCA	2 cut(s)	776, 3136
NspI	RCATGY	3 cut(s)	784, 1240, 2587
NspV	TTCGAA	3 cut(s)	379, 2197, 3085
PaeI	GCATGC	1 cut(s)	2587
PagI	TCATGA	2 cut(s)	756, 3360
PauI	GCGCGC	1 cut(s)	462
PceI	AGGCCT	1 cut(s)	650
PciI	ACATGT	2 cut(s)	780, 1236
PciSI	GCTCTTC	1 cut(s)	1563
PcsI	WCGNNNNNNNCGW	9 cut(s)	228, 276, 285, 288, 297, 330, 339, 371, 676
PctI	GAATGC	2 cut(s)	521, 2255
PdmI	GAANNNNTTC	6 cut(s)	805, 1457, 1559, 1716, 2304, 2954
PflFI	GACNNNGTC	3 cut(s)	2674, 3458, 3484
PleI	GAGTC	7 cut(s)	176, 370, 812, 2353, 2384, 2428, 2635
PmaCI	CACGTG	1 cut(s)	832
PmlI	CACGTG	1 cut(s)	832
PpsI	GAGTC	7 cut(s)	176, 370, 812, 2353, 2384, 2428, 2635
Ppu21I	YACGTR	1 cut(s)	832
PscI	ACATGT	2 cut(s)	780, 1236
PshAI	GACNNNNGTC	1 cut(s)	1761
PsiI	TTATAA	1 cut(s)	2520
Psp124BI	GAGCTC	1 cut(s)	1706
Psp6I	CCWGG	1 cut(s)	3329
PspCI	CACGTG	1 cut(s)	832
PspFI	CCCAGC	2 cut(s)	1727, 2320
PspGI	CCWGG	1 cut(s)	3329
PspN4I	GGNNCC	3 cut(s)	1319, 1460, 2486
PspPI	GGNCC	3 cut(s)	2126, 2235, 3499
PstI	CTGCAG	1 cut(s)	3040
PstNI	CAGNNNCTG	1 cut(s)	751
PsuI	RGATCY	2 cut(s)	2660, 3093
PsyI	GACNNNGTC	3 cut(s)	2674, 3458, 3484
PteI	GCGCGC	1 cut(s)	462
PvuII	CAGCTG	2 cut(s)	2324, 3041
RruI	TCGCGA	1 cut(s)	672
RsaI	GTAC	7 cut(s)	296, 886, 1035, 1228, 1693, 2486, 2694
RsaNI	GTAC	7 cut(s)	295, 885, 1034, 1227, 1692, 2485, 2693
RseI	CAYNNNNRTG	1 cut(s)	834
SacI	GAGCTC	1 cut(s)	1706
SalI	GTCGAC	1 cut(s)	3215
SapI	GCTCTTC	1 cut(s)	1563
Sau96I	GGNCC	3 cut(s)	2126, 2235, 3499
ScaI	AGTACT	1 cut(s)	1228
SchI	GAGTC	7 cut(s)	177, 371, 812, 2354, 2384, 2429, 2636
ScrFI	CCNGG	1 cut(s)	3331
SduI	GDGCHC	2 cut(s)	1144, 1706
SfaNI	GCATC	6 cut(s)	1369, 1468, 1670, 2055, 2080, 2548
SfcI	CTRYAG	7 cut(s)	597, 606, 1608, 2153, 2760, 3036, 3114
SfuI	TTCGAA	3 cut(s)	379, 2197, 3085
SinI	GGWCC	2 cut(s)	2126, 3499
SmiMI	CAYNNNNRTG	1 cut(s)	834
SmlI	CTYRAG	6 cut(s)	526, 644, 1296, 1322, 2366, 2666
SmoI	CTYRAG	6 cut(s)	526, 644, 1296, 1322, 2366, 2666
SpeI	ACTAGT	1 cut(s)	1202
SphI	GCATGC	1 cut(s)	2587
SseBI	AGGCCT	1 cut(s)	650
SstI	GAGCTC	1 cut(s)	1706
StuI	AGGCCT	1 cut(s)	650
StyD4I	CCNGG	1 cut(s)	3329
StyI	CCWWGG	2 cut(s)	3075, 3170
TaiI	ACGT	9 cut(s)	282, 429, 834, 1693, 1744, 2675, 2800, 2911, 3222
TatI	WGTACW	1 cut(s)	1226
TauI	GCSGC	5 cut(s)	203, 287, 1385, 2462, 3409
TscAI	CASTG	4 cut(s)	894, 1044, 1144, 1690
TseFI	GTSAC	5 cut(s)	169, 869, 1679, 2346, 2739
TseI	GCWGC	8 cut(s)	590, 593, 1523, 1622, 2024, 3038, 3041, 3125
Tsp45I	GTSAC	5 cut(s)	169, 869, 1679, 2346, 2739
TspGWI	ACGGA	2 cut(s)	1213, 1766
TspRI	CASTG	4 cut(s)	894, 1044, 1144, 1690
Tth111I	GACNNNGTC	3 cut(s)	2674, 3458, 3484
Vha464I	CTTAAG	3 cut(s)	644, 1322, 2366
VpaK11BI	GGWCC	2 cut(s)	2126, 3499
XapI	RAATTY	5 cut(s)	389, 674, 1186, 3162, 3432
XbaI	TCTAGA	1 cut(s)	1241
XceI	RCATGY	3 cut(s)	784, 1240, 2587
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3272
XmiI	GTMKAC	3 cut(s)	1543, 2853, 3216
XmnI	GAANNNNTTC	6 cut(s)	805, 1457, 1559, 1716, 2304, 2954
ZraI	GACGTC	3 cut(s)	280, 427, 2909
ZrmI	AGTACT	1 cut(s)	1228

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.