Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0013378

Physical Location & Seq

Genomic Coordinates Forward (+)

57223042 .. 57228327

Gene Structure 5286 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG429400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2775 bp

ATGACCACAGAGATTTTGCGCAACATGTTGTATCAGAGCGTTGGAATGTCTTCTGCTCGGGACTCACTTTTTCACGTGGATGTAATAGTCTTGGATGAAGTTCATTATTTAAGTGACATATCTCGGGGTACAGTGTGGGAAGAAATTGTTATTTATTCCCCAAAAGTGGTTCAACTCATATGTTTGTCCGCAACAGTTGCTAATCCAGATGAATTGGCTGGTTGGATTGGTCAGATTCATGGTAAAACTGAATTGGTAACTTCAACAAAGCGTCCAGTACCACTGACTTGGCATTTCTCGACAAAGACTTCTTTATTACCACTTCTTGATAAGACAGGAAAACACATGAATAGGAGGTTGTCAGTCAATTATCTGCAACTTAGTGCTGCTGCAACAAAATCTAATAAAGATGATGGATCTAGAAGGAGGAGTTCAAGAAGACGGGCTAGTGAGACGAGCTATGATGACAGTACTGGTAACATGTCTAGACGACCACTTTCAAAGAATGACATCAACTTAATATACCGTTCACAAGTGCCTCAAGTTACTGATACATTATGGCACCTTAAGGCTAGGGATATGCTACCAGCAGTATGGTTTATTTTTAGCAGGAAAGGTTGTGATGCTGCTGTTCAGTACATTCAAGATTGCAAACTTCTAGATGACTGTGAAGCAAGTGAGGTTGAATTAGCGTTGAAGAGGTTCCGCATTAAGTATCCGGATGCTATCAGGGAGAGTGCAGTGAAGGGGTTACTGCGAGGGGTTGCTGCCCATCATGCTGGCTGTCTACCTTTATGGAAATCATTCATAGAAGAGCTGTTTCAGCGTGGTCTTGTTAAGGTTGTATTTGCTACAGAAACACTTGCTGCTGGAATCAATATGCCTGCTAGGACAGCCATTATTGCATCCCTCAGCAAAAGGAGTGACAGTGGACGTACCCTATTGAGCTCAAATGAACTACTTCAAATGGCTGGCCGTGCTGGACGTAGAGGCACAGATGAAAGGGGTCATGTAGTGCTTATTCAAAATCCTTACGAGGGTGCTGAAGCTGGTTGCAAGGTTCTTTTTGCTGGACTTGAACCTCTGGTATCACAGTTTACTGCTTCATATGGAATGGTGCTAAATCTTCTTGCCGGTTCAAAAGTCACTCGTAGATCCAGTGAATCAGATGATACAAAAGCCTCCCAACCTGGGCGAACTTTGGACGAAGCTAGAAAGCTAGTTGAGCAAAGTTTTGGAAACTATGTTGGCAGCAATGTTATGGTTGCTGCAAAAGAGGAGCTTGCTAGAATTGAGAAAGAAATAGAGATGCTGACTTTGGAAATTAGCGATGATGCCATCGACAGAAAAAGCAGGAAGCTCTTGTCTGGACCAGCATACAAGGAGATTGCTGATCTACAGGAAGAATTAAGGGCAGAGAAACGCCTAAGGACTGAACTTCGGAAAAGAATGGAATCTCAGAAGCTTTCTTCACTACGACCCTTATTAAAGGAGTGTGAAAATGGGCACTTGCCCTTTTTGTGCTTGCAGTATAAAGATTCAGAAGGAGTTCAACACTCCATCCCGACTGTTTATTTGGGGAAGGTTGAGTTGCTGAGTTGCTCAAAGCTTAAGAACATGGTTTCTGTGGACGATTCTTTTGCACTAAATGCAGTAGCAGCAGAGTCTGAACCAACTTCAGTTTTTGAACCATCATATTATGCGGCTCTTGGCTCTGACAATTCTTGGTACCTATTTACAGAAAAGTGGATCAAAACAATTTATAAGACAGGCTTTCCAAATGTTGCTTTAGCTCAAGGAGATGCTTTGCCTCGGGAAATAATGAGCACGCTTCTTGATAAGACGGAAATGAAGTGGGAGAAGCTTGCGGAGTCTGATCTTGGTGGTTTTTGGGGCATGGAAGGATCTCTTGAGACATGGTCTTGGAGTTTGAATGTTCCAGTCTTGAATAGTCTTTCTGAACATGATGAGCTTTTACTCAAGTCAGAAGCATATAATCATGCTATAGGGTGTTATAAAGAACAAAGAAGTAAAGTTTCACGTCTGAAGAAAAAGATATCGCGTACACAAGGCTTCAGAGAATACAAGAAGATCATTGACATGGCCAACTATACAGAGGAGAAGATCAAGCGCTTGAAGGGTAGATCAAGACGTTTGACCAATCGAATAGGGCAGATTGAACCATCTGGTTGGAAAGAGTTCTTGCAGATCAGCAATGTTATACATGAAACTCGGGCATTAGATATTAACACTCATACCATATTTCCACTTGGTGAAACTGCAGCCGCTATTCGAGGAGAAAATGAACTGTGGCTTGCCATGGTTCTTCGAAATAAGATCTTACTAAGTTTAAAGCCTACAGAACTTGCTGCTGTGTGCGCCAGTTTAGTATCTGAAGGGATCAAAATTCGTCCGTGGAAAAATAACAGCTACATATACGAACCTTCAAGTACAGTAATTGACGTGGTGAGCTTCTTGGATGAGCAAAGAAGCACTTTTTTACAGCTTCAGGAAAAGCATGGGGTAAATAAGCCTTGCTATTTGGATACCCAGTTTGCAGGTATGGTTGAAGCCTGGGTTTCTGGGTTGACTTGGAGGGAAATCATGATGGATTGTGCAATGGACGAGGGAGATTTAGCCCGCCTTCTACGGCGAACTATAGATCTGCTGGTGCAAATCCCCAAATTGCCTGATATTGACCCCGTCTTGCAGAGCAACGCAAAGACGGCGTCAAATATCATGGACCGCCCTCCGATTAGTGAGTTAGCTGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000373 GO:0000375 GO:0000377 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006810 GO:0007275 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009892 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010494 GO:0010496 GO:0010497 GO:0010501 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035770 GO:0036464 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0051179 GO:0051234 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C

Protein Analysis

924

Amino Acids

103.87

Weight (kDa)

8.44

Isoelectric Point (pI)

49.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	2 - 70	1.2e-06	DEAD/DEAH box helicase
SH3_ISE2	PF25446	488 - 666	1.2e-86	ISE2, SH3 domain
DSHCT	PF08148	753 - 919	1.9e-34	DSHCT (NUC185) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013378)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70070
fragaria_vesca	FvH4_4g35290 FvH4_4g35290 FvH4_4g35290
malus_domestica	MD16G1083800.v1.1
prunus_persica	Prupe.1G262200_v2.0.a1
pyrus_communis	pycom16g07280
rosa_chinensis	RchiOBHm_Chr4g0444891
rosa_laevigata	RLG00000005791
rosa_multiflora	Rmu_sc0006845.1_g000003 Rmu_sc0013783.1_g000003
rosa_roxburghii	Rroxscaffold_5G00385390
rosa_rugosa	Rorug04G0355800
rosa_samantha	Rh4AG417600 Rh4BG429400 Rh4CG444100 Rh4DG425300
rosa_wichuraiana	Rw4G035790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1764, 2016
Acc16I	TGCGCA	1 cut(s)	20
Acc36I	ACCTGC	1 cut(s)	2549
Acc65I	GGTACC	1 cut(s)	1728
AccB1I	GGYRCC	2 cut(s)	561, 1728
AccI	GTMKAC	1 cut(s)	787
AccII	CGCG	1 cut(s)	2062
AccIII	TCCGGA	1 cut(s)	718
AciI	CCGC	7 cut(s)	189, 706, 1703, 1868, 2286, 2641, 2746
AclWI	GGATC	5 cut(s)	424, 1149, 1757, 1912, 2408
AcoI	YGGCCR	2 cut(s)	973, 2103
AcsI	RAATTY	1 cut(s)	2406
AcuI	CTGAAG	6 cut(s)	1065, 1662, 2059, 2066, 2415, 2492
AcvI	CACGTG	1 cut(s)	76
AcyI	GRCGYC	1 cut(s)	2729
AdeI	CACNNNGTG	1 cut(s)	2273
AfaI	GTAC	8 cut(s)	130, 279, 472, 638, 937, 1730, 2065, 2452
AfeI	AGCGCT	1 cut(s)	2132
AfiI	CCNNNNNNNGG	3 cut(s)	166, 1037, 1191
AflII	CTTAAG	2 cut(s)	566, 1610
AflIII	ACRYGT	2 cut(s)	24, 480
AjiI	CACGTC	2 cut(s)	2042, 2464
AjnI	CCWGG	2 cut(s)	1189, 2573
Alw21I	GWGCWC	2 cut(s)	950, 1829
Alw26I	GTCTC	2 cut(s)	446, 1907
AlwI	GGATC	5 cut(s)	424, 1149, 1757, 1912, 2408
AlwNI	CAGNNNCTG	1 cut(s)	1667
Ama87I	CYCGRG	4 cut(s)	57, 123, 1812, 2232
Aor13HI	TCCGGA	1 cut(s)	718
Aor51HI	AGCGCT	1 cut(s)	2132
AoxI	GGCC	2 cut(s)	973, 2103
ApoI	RAATTY	1 cut(s)	2406
ArsI	GACNNNNNNTTYG	6 cut(s)	1558, 1590, 1622, 1654, 2714, 2746
Asp700I	GAANNNNTTC	6 cut(s)	49, 701, 803, 960, 1548, 2198
Asp718I	GGTACC	1 cut(s)	1728
AspLEI	GCGC	3 cut(s)	21, 2133, 2381
AspS9I	GGNCC	2 cut(s)	1370, 2743
AsuHPI	GGTGA	2 cut(s)	2285, 2479
AsuII	TTCGAA	1 cut(s)	2329
AvaI	CYCGRG	4 cut(s)	57, 123, 1812, 2232
AvaII	GGWCC	2 cut(s)	1370, 2743
AxyI	CCTNAGG	1 cut(s)	1427
BaeGI	GKGCMC	1 cut(s)	1509
BalI	TGGCCA	1 cut(s)	2105
BanI	GGYRCC	2 cut(s)	561, 1728
BanII	GRGCYC	1 cut(s)	950
BbrPI	CACGTG	1 cut(s)	76
BbsI	GAAGAC	2 cut(s)	42, 445
Bbv12I	GWGCWC	2 cut(s)	950, 1829
BbvCI	CCTCAGC	1 cut(s)	911
BccI	CCATC	7 cut(s)	407, 780, 1346, 1568, 1699, 2191, 2602
BceAI	ACGGC	3 cut(s)	960, 2666, 2742
BcgI	CGANNNNNNTGC	2 cut(s)	1622, 1656
BciT130I	CCWGG	2 cut(s)	1191, 2575
BciVI	GTATCC	2 cut(s)	726, 2539
BcoDI	GTCTC	2 cut(s)	446, 1907
BfaI	CTAG	9 cut(s)	420, 447, 486, 573, 659, 888, 1212, 1220, 1287
BfmI	CTRYAG	6 cut(s)	852, 1397, 2004, 2280, 2358, 2658
BfoI	RGCGCY	1 cut(s)	2134
BfrI	CTTAAG	2 cut(s)	566, 1610
BfuAI	ACCTGC	1 cut(s)	2549
BfuI	GTATCC	2 cut(s)	726, 2539
BglII	AGATCT	2 cut(s)	2337, 2662
BmcAI	AGTACT	1 cut(s)	472
Bme1390I	CCNGG	2 cut(s)	1191, 2575
Bme18I	GGWCC	2 cut(s)	1370, 2743
BmeT110I	CYCGRG	4 cut(s)	57, 123, 1812, 2232
BmgBI	CACGTC	2 cut(s)	2042, 2464
BmgT120I	GGNCC	2 cut(s)	1370, 2743
BmiI	GGNNCC	3 cut(s)	563, 704, 1730
BmrFI	CCNGG	2 cut(s)	1191, 2575
BmrI	ACTGGG	1 cut(s)	2545
BmsI	GCATC	6 cut(s)	613, 712, 914, 1299, 1324, 1792
BmuI	ACTGGG	1 cut(s)	2545
BpiI	GAAGAC	2 cut(s)	42, 445
Bpu10I	CCTNAGC	1 cut(s)	911
Bpu14I	TTCGAA	1 cut(s)	2329
BpuEI	CTTGAG	4 cut(s)	525, 1779, 1931, 1964
BsaAI	YACGTR	1 cut(s)	76
BsaHI	GRCGYC	1 cut(s)	2729
BsaJI	CCNNGG	5 cut(s)	1190, 1811, 2319, 2414, 2574
BsaWI	WCCGGW	1 cut(s)	718
BsaXI	ACNNNNNCTCC	2 cut(s)	913, 943
Bsc4I	CCNNNNNNNGG	3 cut(s)	166, 1037, 1191
Bse118I	RCCGGY	1 cut(s)	1133
Bse1I	ACTGG	6 cut(s)	275, 478, 1158, 1940, 2382, 2551
Bse21I	CCTNAGG	1 cut(s)	1427
Bse3DI	GCAATG	3 cut(s)	1261, 2221, 2625
BseAI	TCCGGA	1 cut(s)	718
BseBI	CCWGG	2 cut(s)	1191, 2575
BseDI	CCNNGG	5 cut(s)	1190, 1811, 2319, 2414, 2574
BseGI	GGATG	6 cut(s)	85, 100, 727, 905, 1560, 2485
BseLI	CCNNNNNNNGG	3 cut(s)	166, 1037, 1191
BseMI	GCAATG	3 cut(s)	1261, 2221, 2625
BseMII	CTCAG	3 cut(s)	925, 1472, 1586
BseNI	ACTGG	6 cut(s)	275, 478, 1158, 1940, 2382, 2551
BseRI	GAGGAG	4 cut(s)	442, 1292, 2132, 2310
BseSI	GKGCMC	1 cut(s)	1509
BsgI	GTGCAG	1 cut(s)	759
Bsh1236I	CGCG	1 cut(s)	2062
BshFI	GGCC	2 cut(s)	975, 2105
BshNI	GGYRCC	2 cut(s)	561, 1728
BsiHKAI	GWGCWC	2 cut(s)	950, 1829
BsiHKCI	CYCGRG	4 cut(s)	57, 123, 1812, 2232
BsiSI	CCGG	2 cut(s)	719, 1134
BslFI	GGGAC	1 cut(s)	74
BslI	CCNNNNNNNGG	3 cut(s)	166, 1037, 1191
BsmAI	GTCTC	2 cut(s)	446, 1907
BsmBI	CGTCTC	1 cut(s)	446
BsmFI	GGGAC	1 cut(s)	74
BsnI	GGCC	2 cut(s)	975, 2105
BsoBI	CYCGRG	4 cut(s)	57, 123, 1812, 2232
Bsp119I	TTCGAA	1 cut(s)	2329
Bsp1286I	GDGCHC	3 cut(s)	950, 1509, 1829
Bsp13I	TCCGGA	1 cut(s)	718
Bsp19I	CCATGG	1 cut(s)	2319
BspACI	CCGC	7 cut(s)	189, 706, 1703, 1868, 2286, 2641, 2746
BspANI	GGCC	2 cut(s)	975, 2105
BspCNI	CTCAG	3 cut(s)	924, 1471, 1587
BspEI	TCCGGA	1 cut(s)	718
BspFNI	CGCG	1 cut(s)	2062
BspHI	TCATGA	1 cut(s)	2604
BspLI	GGNNCC	3 cut(s)	563, 704, 1730
BspMAI	CTGCAG	1 cut(s)	2284
BspMI	ACCTGC	1 cut(s)	2549
BspPI	GGATC	5 cut(s)	424, 1149, 1757, 1912, 2408
BspQI	GCTCTTC	1 cut(s)	807
BspT104I	TTCGAA	1 cut(s)	2329
BspT107I	GGYRCC	2 cut(s)	561, 1728
BspTI	CTTAAG	2 cut(s)	566, 1610
BsrDI	GCAATG	3 cut(s)	1261, 2221, 2625
BsrFI	RCCGGY	1 cut(s)	1133
BsrI	ACTGG	6 cut(s)	275, 478, 1158, 1940, 2382, 2551
BssAI	RCCGGY	1 cut(s)	1133
BssECI	CCNNGG	5 cut(s)	1190, 1811, 2319, 2414, 2574
BssNI	GRCGYC	1 cut(s)	2729
BssT1I	CCWWGG	1 cut(s)	2319
Bst2UI	CCWGG	2 cut(s)	1191, 2575
Bst6I	CTCTTC	2 cut(s)	692, 807
BstACI	GRCGYC	1 cut(s)	2729
BstAFI	CTTAAG	2 cut(s)	566, 1610
BstAPI	GCANNNNNTGC	2 cut(s)	197, 1649
BstBAI	YACGTR	1 cut(s)	76
BstBI	TTCGAA	1 cut(s)	2329
BstC8I	GCNNGC	9 cut(s)	781, 885, 973, 1284, 1526, 1829, 1866, 2316, 2641
BstDEI	CTNAG	6 cut(s)	380, 911, 1427, 1458, 1595, 2345
BstDSI	CCRYGG	2 cut(s)	2319, 2414
BstF5I	GGATG	6 cut(s)	85, 100, 727, 905, 1560, 2485
BstFNI	CGCG	1 cut(s)	2062
BstH2I	RGCGCY	1 cut(s)	2134
BstHHI	GCGC	3 cut(s)	21, 2133, 2381
BstMAI	GTCTC	2 cut(s)	446, 1907
BstNI	CCWGG	2 cut(s)	1191, 2575
BstNSI	RCATGY	2 cut(s)	28, 484
BstSCI	CCNGG	2 cut(s)	1189, 2573
BstSFI	CTRYAG	6 cut(s)	852, 1397, 2004, 2280, 2358, 2658
BstSLI	GKGCMC	1 cut(s)	1509
BstUI	CGCG	1 cut(s)	2062
BstV2I	GAAGAC	2 cut(s)	42, 445
BstX2I	RGATCY	5 cut(s)	416, 1154, 1904, 2337, 2662
BstXI	CCANNNNNNTGG	4 cut(s)	288, 594, 779, 2190
BstYI	RGATCY	5 cut(s)	416, 1154, 1904, 2337, 2662
Bsu36I	CCTNAGG	1 cut(s)	1427
BsuI	GTATCC	2 cut(s)	726, 2539
BsuRI	GGCC	2 cut(s)	975, 2105
BtgI	CCRYGG	2 cut(s)	2319, 2414
BtgZI	GCGATG	1 cut(s)	1344
BtrI	CACGTC	2 cut(s)	2042, 2464
BtsCI	GGATG	6 cut(s)	85, 100, 727, 905, 1560, 2485
BtsI	GCAGTG	1 cut(s)	747
BtsIMutI	CAGTG	5 cut(s)	138, 281, 747, 934, 1165
BveI	ACCTGC	1 cut(s)	2549
Cac8I	GCNNGC	9 cut(s)	781, 885, 973, 1284, 1526, 1829, 1866, 2316, 2641
CaiI	CAGNNNCTG	1 cut(s)	1667
CciI	TCATGA	1 cut(s)	2604
CfoI	GCGC	3 cut(s)	21, 2133, 2381
Cfr10I	RCCGGY	1 cut(s)	1133
Cfr13I	GGNCC	2 cut(s)	1370, 2743
CseI	GACGC	2 cut(s)	260, 2718
Csp6I	GTAC	8 cut(s)	129, 278, 471, 637, 936, 1729, 2064, 2451
CviQI	GTAC	8 cut(s)	129, 278, 471, 637, 936, 1729, 2064, 2451
DdeI	CTNAG	6 cut(s)	380, 911, 1427, 1458, 1595, 2345
DraI	TTTAAA	1 cut(s)	2352
DraIII	CACNNNGTG	1 cut(s)	2273
EaeI	YGGCCR	2 cut(s)	973, 2103
Eam1104I	CTCTTC	2 cut(s)	692, 807
EarI	CTCTTC	2 cut(s)	692, 807
Ecl136II	GAGCTC	1 cut(s)	948
Eco130I	CCWWGG	1 cut(s)	2319
Eco24I	GRGCYC	1 cut(s)	950
Eco32I	GATATC	1 cut(s)	2058
Eco47I	GGWCC	2 cut(s)	1370, 2743
Eco47III	AGCGCT	1 cut(s)	2132
Eco53kI	GAGCTC	1 cut(s)	948
Eco57I	CTGAAG	6 cut(s)	1065, 1662, 2059, 2066, 2415, 2492
Eco72I	CACGTG	1 cut(s)	76
Eco81I	CCTNAGG	1 cut(s)	1427
Eco88I	CYCGRG	4 cut(s)	57, 123, 1812, 2232
EcoICRI	GAGCTC	1 cut(s)	948
EcoRII	CCWGG	2 cut(s)	1189, 2573
EcoRV	GATATC	1 cut(s)	2058
EcoT14I	CCWWGG	1 cut(s)	2319
EcoT38I	GRGCYC	1 cut(s)	950
ErhI	CCWWGG	1 cut(s)	2319
Esp3I	CGTCTC	1 cut(s)	446
FalI	AAGNNNNNCTT	8 cut(s)	1266, 1298, 1757, 1789, 1893, 1925, 2479, 2511
FaqI	GGGAC	1 cut(s)	74
FauI	CCCGC	1 cut(s)	2648
FauNDI	CATATG	2 cut(s)	179, 1108
FblI	GTMKAC	1 cut(s)	787
FokI	GGATG	6 cut(s)	92, 107, 734, 892, 1547, 2492
FriOI	GRGCYC	1 cut(s)	950
FspBI	CTAG	9 cut(s)	420, 447, 486, 573, 659, 888, 1212, 1220, 1287
FspI	TGCGCA	1 cut(s)	20
GlaI	GCGC	3 cut(s)	20, 2132, 2380
HaeII	RGCGCY	1 cut(s)	2134
HaeIII	GGCC	2 cut(s)	975, 2105
HapII	CCGG	2 cut(s)	719, 1134
HgaI	GACGC	2 cut(s)	260, 2718
HhaI	GCGC	3 cut(s)	21, 2133, 2381
Hin1I	GRCGYC	1 cut(s)	2729
Hin6I	GCGC	3 cut(s)	19, 2131, 2379
HinP1I	GCGC	3 cut(s)	19, 2131, 2379
HincII	GTYRAC	1 cut(s)	2589
HindII	GTYRAC	1 cut(s)	2589
HindIII	AAGCTT	3 cut(s)	1463, 1607, 1862
HinfI	GANTC	9 cut(s)	62, 235, 873, 1163, 1454, 1538, 1634, 1664, 1871
HpaII	CCGG	2 cut(s)	719, 1134
HphI	GGTGA	2 cut(s)	2285, 2479
Hpy166II	GTNNAC	7 cut(s)	530, 788, 932, 1098, 1630, 2066, 2589
Hpy8I	GTNNAC	7 cut(s)	530, 788, 932, 1098, 1630, 2066, 2589
HpyAV	CCTTC	9 cut(s)	417, 739, 1538, 1576, 1895, 2131, 2390, 2454, 2654
HpyCH4IV	ACGT	6 cut(s)	75, 934, 985, 2041, 2152, 2463
HpyF3I	CTNAG	6 cut(s)	380, 911, 1427, 1458, 1595, 2345
HpySE526I	ACGT	6 cut(s)	75, 934, 985, 2041, 2152, 2463
Hsp92I	GRCGYC	1 cut(s)	2729
HspAI	GCGC	3 cut(s)	19, 2131, 2379
Kpn2I	TCCGGA	1 cut(s)	718
KpnI	GGTACC	1 cut(s)	1732
LguI	GCTCTTC	1 cut(s)	807
LmnI	GCTCC	1 cut(s)	1279
LweI	GCATC	6 cut(s)	613, 712, 914, 1299, 1324, 1792
MaeI	CTAG	9 cut(s)	420, 447, 486, 573, 659, 888, 1212, 1220, 1287
MaeII	ACGT	6 cut(s)	75, 934, 985, 2041, 2152, 2463
MaeIII	GTNAC	7 cut(s)	113, 256, 476, 544, 750, 923, 1144
MflI	RGATCY	5 cut(s)	416, 1154, 1904, 2337, 2662
MhlI	GDGCHC	3 cut(s)	950, 1509, 1829
MlsI	TGGCCA	1 cut(s)	2105
MluNI	TGGCCA	1 cut(s)	2105
MlyI	GAGTC	3 cut(s)	56, 1673, 1880
MmeI	TCCRAC	3 cut(s)	22, 203, 2171
Mox20I	TGGCCA	1 cut(s)	2105
MroI	TCCGGA	1 cut(s)	718
MroXI	GAANNNNTTC	6 cut(s)	49, 701, 803, 960, 1548, 2198
MscI	TGGCCA	1 cut(s)	2105
MslI	CAYNNNNRTG	2 cut(s)	78, 2099
Msp20I	TGGCCA	1 cut(s)	2105
MspCI	CTTAAG	2 cut(s)	566, 1610
MspI	CCGG	2 cut(s)	719, 1134
MspR9I	CCNGG	2 cut(s)	1191, 2575
MvaI	CCWGG	2 cut(s)	1191, 2575
MvnI	CGCG	1 cut(s)	2062
NcoI	CCATGG	1 cut(s)	2319
NdeI	CATATG	2 cut(s)	179, 1108
NlaIV	GGNNCC	3 cut(s)	563, 704, 1730
NmuCI	GTSAC	3 cut(s)	113, 923, 1144
NsbI	TGCGCA	1 cut(s)	20
NspI	RCATGY	2 cut(s)	28, 484
NspV	TTCGAA	1 cut(s)	2329
PagI	TCATGA	1 cut(s)	2604
PciI	ACATGT	2 cut(s)	24, 480
PciSI	GCTCTTC	1 cut(s)	807
PdmI	GAANNNNTTC	6 cut(s)	49, 701, 803, 960, 1548, 2198
PfeI	GAWTC	6 cut(s)	235, 873, 1163, 1454, 1538, 1634
PflFI	GACNNNGTC	3 cut(s)	1918, 2702, 2728
PleI	GAGTC	3 cut(s)	56, 1672, 1879
PmaCI	CACGTG	1 cut(s)	76
PmlI	CACGTG	1 cut(s)	76
PpsI	GAGTC	3 cut(s)	56, 1672, 1879
Ppu21I	YACGTR	1 cut(s)	76
PscI	ACATGT	2 cut(s)	24, 480
PsiI	TTATAA	2 cut(s)	1764, 2016
Psp124BI	GAGCTC	1 cut(s)	950
Psp6I	CCWGG	2 cut(s)	1189, 2573
PspCI	CACGTG	1 cut(s)	76
PspGI	CCWGG	2 cut(s)	1189, 2573
PspN4I	GGNNCC	3 cut(s)	563, 704, 1730
PspPI	GGNCC	2 cut(s)	1370, 2743
PstI	CTGCAG	1 cut(s)	2284
PstNI	CAGNNNCTG	1 cut(s)	1667
PsuI	RGATCY	5 cut(s)	416, 1154, 1904, 2337, 2662
PsyI	GACNNNGTC	3 cut(s)	1918, 2702, 2728
RsaI	GTAC	8 cut(s)	130, 279, 472, 638, 937, 1730, 2065, 2452
RsaNI	GTAC	8 cut(s)	129, 278, 471, 637, 936, 1729, 2064, 2451
RseI	CAYNNNNRTG	2 cut(s)	78, 2099
SacI	GAGCTC	1 cut(s)	950
SapI	GCTCTTC	1 cut(s)	807
Sau96I	GGNCC	2 cut(s)	1370, 2743
ScaI	AGTACT	1 cut(s)	472
SchI	GAGTC	3 cut(s)	56, 1673, 1880
ScrFI	CCNGG	2 cut(s)	1191, 2575
SduI	GDGCHC	3 cut(s)	950, 1509, 1829
SfaNI	GCATC	6 cut(s)	613, 712, 914, 1299, 1324, 1792
SfcI	CTRYAG	6 cut(s)	852, 1397, 2004, 2280, 2358, 2658
SfuI	TTCGAA	1 cut(s)	2329
SinI	GGWCC	2 cut(s)	1370, 2743
SmiMI	CAYNNNNRTG	2 cut(s)	78, 2099
SmlI	CTYRAG	6 cut(s)	540, 566, 1610, 1794, 1910, 1979
SmoI	CTYRAG	6 cut(s)	540, 566, 1610, 1794, 1910, 1979
SsiI	CCGC	7 cut(s)	189, 706, 1703, 1868, 2286, 2641, 2746
SspMI	CTAG	9 cut(s)	420, 447, 486, 573, 659, 888, 1212, 1220, 1287
SstI	GAGCTC	1 cut(s)	950
StyD4I	CCNGG	2 cut(s)	1189, 2573
StyI	CCWWGG	1 cut(s)	2319
TaiI	ACGT	6 cut(s)	78, 937, 988, 2044, 2155, 2466
TaqI	TCGA	5 cut(s)	299, 1341, 2164, 2293, 2329
TatI	WGTACW	3 cut(s)	470, 636, 2450
TauI	GCSGC	2 cut(s)	1706, 2288
TfiI	GAWTC	6 cut(s)	235, 873, 1163, 1454, 1538, 1634
TscAI	CASTG	5 cut(s)	138, 288, 747, 934, 1165
TseFI	GTSAC	3 cut(s)	113, 923, 1144
Tsp45I	GTSAC	3 cut(s)	113, 923, 1144
TspGWI	ACGGA	2 cut(s)	1859, 2403
TspRI	CASTG	5 cut(s)	138, 288, 747, 934, 1165
Tth111I	GACNNNGTC	3 cut(s)	1918, 2702, 2728
Vha464I	CTTAAG	2 cut(s)	566, 1610
VpaK11BI	GGWCC	2 cut(s)	1370, 2743
XapI	RAATTY	1 cut(s)	2406
XbaI	TCTAGA	3 cut(s)	419, 485, 658
XceI	RCATGY	2 cut(s)	28, 484
XmiI	GTMKAC	1 cut(s)	787
XmnI	GAANNNNTTC	6 cut(s)	49, 701, 803, 960, 1548, 2198
XspI	CTAG	9 cut(s)	420, 447, 486, 573, 659, 888, 1212, 1220, 1287
ZrmI	AGTACT	1 cut(s)	472

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.