Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0013378

Physical Location & Seq

Genomic Coordinates Reverse (-)

5881457 .. 5886259

Gene Structure 4803 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1083800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3528 bp

ATGGACACTCTCTCCATCCTCACACCCTCCGACATCTTCACGCGCCTCCTTCCTCTCAGCCGCGCTTCCCATCCCCTCCCCCGCCACCCCACCAAAACCCTAGGGTTTTGCTCCCCCAAACCACCTCCCGCGCACTCTCCGACCTCACCGCGATTCCGAGTCAGATTCCAGCGCCCGAGCTCCGCCTTCCCCGCCAAATCTCAGCTCTACGATGCCGATGAAGAAGAGGAGGAAGACGAGTACGAAGAGGAGGAGGACGACGACGAGGACGTTGCCGCGGATGAGTACGACGACGTTACTGGGGACATGTCGGACGGACTCGAGCAGAGCGACGACGAAATTGAGAGTTCGGTGGCTGACGCAGAGCCGTCGACTCGGCCGGAGGAGTTCAAGTGGCAGAGAGTGGAGAAGCTCTGCGGCGAAGTCAAAGTGTTCGGCGAGGAAATGATAGATGACCACGAGCTCGCTTCTATCTACGATTTCCGAATCGATAAGTTTCAGAGATTAGCGATACAAGCGTTCTTGAGAGGTTCGTCGGTGGTGGTATCGGCGCCGACGAGCAGTGGAAAGACTTTGATAGCGGAAGCTGCCGCCGTCGCAACGGTGGCTAGGCGGAGGAGGTTGTTCTACACGACTCCACTTAAGGCCTTGTCCAATCAGAAGTTTCGGGAATTTCGTGAGACTTTTGGCGATGACAATGTCGGCCTTCTCACCGGAGATTCTGCGGTAAATAAAGATGCTCAGGTTTTAGTTATGACCACTGAGATCTTGCGCAACATGTTGTATCAGAGTGTGGGAATGGCTTCTTCTGGGGACGGACTTTTTCACGTAGATGTCATTGTCTTGGATGAAGTTCATTATCTGAGTGACATGTATCGGGGTACAGTGTGGGAAGAAATCGTTATATATTGCCCGAAAAAAGTTCAACTCATATGTTTGTCAGCAACAGTAGCAAACCCAGATGAATTGGCTGGTTGGATTGGTCAGATTCATGGTAAAACTGAATTGGTAACTTCATCAAAGCGTCCAGTACCATTGACTTGGCATTTCTCGACAAAGACTTCTTTGTTGCATCTTCTTGACAAGACGGGAAAACACATGAATAGGAGGTTGTCAGTCGATTATCTTCAACTCAATGCTTCAGGAACAAAATCTTACAAAGACGATGGCTCTAGAAGGAGGGGCTCAAGAAGACGTGGAAATGAGATGAGTTATGATGACAGTAATGGTAACATGTCTAGACGACCACTGTCAAAGAATGACATCAACTTAATACACCGTTCGCAAGTACCTCAAATTACCGATACATTATGGCAACTTAAGACTCGGGATATGCTTCCAGCAATATGGTTTATTTTTAGTAGGAAAGGATGTGATGCTGCTGTTCAGTATGTTCAAGATATCACGCTCTTGGATGACTGTGAGAAGAGTGAGGTTGAATTAGCCTTGAAGAGGTTCCGCATCAAGTATCCTGATGCTATCAGGGAGACTGCTGTGAAAGGTTTACTGCATGGGGTTGCTGCACATCATGCTGGCTGTCTACCTTTATGGAAATCATTCATAGAAGAGCTGTTTCAGCGGGGGCTTGTTAAGGTCGTCTTTGCAACAGAAACACTTGCTGCAGGAATCAATATGCCTGCTAGGACAGCCATTATTGCATCCCTCAGCAAAAGAGGTGACACTGGACGTGTCCAATTAAGTCCAAACGAACTGTTTCAAATGGCTGGGCGTGCTGGACGTAGAGGCATTGATGAAAAGGGTCATGTAGTGCTTGTTCAAAGTCCTTATGAAGGCGCTGAAGCAGGTTGCAAGATTGTTTTTGCTGGACTTGAACCTTTGGTCTCGCAGTTTACAGCTTCATATGGAATGGTGCTGAATCTTCTTGCTGGTGCAAAAGTTACTCATAGATCAAATGAACCAGATGACACTGAAGCCTCCCTATCAGGGCGAACTTTGGAAGAAGCTAGAAAGTTAGTCGAGCAAAGTTTCGGAAACTATGTTGGCAGCAATGTTATGATTGCCGCAAAAGAGGAGCTTACTAGAATTCAAAAAGAGATTGAGACACTGTCTTCAGAGATTAGCGATGAAGCAATTGATAGAAGGAGTAGGAAGATATTATCTGGACCAGCATATAAGGAGATTGCAGATCTACAAGAGGAATTAAGAGCAGAGAAACGACTGAGGACCGAACTTCGAAGAAGAATGGAATCTCAAAAAATGTCTTCACTACGACCGTTGTTAGAGGAGTTTGAAAACGGACAATTACCCTTTTTGTGCTTGCAGTACAAGGATTCTGAAGGAGTTCAACAGTCTATCCCGGCTGTTTATTTGGGGAAAGTTGACACGGTCAACAGTTCAAAGCTCAAGCACTTGGTTTCTGTTGATGATTCATTTGCACTAAATGCAGTGGCCTGTGAGTTTGAACCAAATGTGGTTTTCGAACCATCATATTACGTGGCTCTTGGTTCAGATAATTCCTGGTATTTGTTTACAGAAAAGTGGATTAAAACAGTTTATAAGACGGGTTTCCCAAATGTTGCTTTAGCGCTAGGAGATGCTTTGCCTCGGGAAATTATGAGCACGCTTCTGGATAAGACGGAGCTGAAGTGGGAGAAGCTTGCCGAGTCTGAACTCGGCGGTTTTTGGAACATGGAAGGATCTCTTGAGACATGGTCTTGGAGTTTGAATGTTCCAGTCTTGAATAGTCTTTCTGAACATGACGAGCTTTTGCATAAGTCAGAAGCATACAATGAAGCAGTAGAACGTTACAAAGATCAAAGAAACAAGGTTTCACGTCTGAAGAAGAAGATCTCGCGTACGCAAGGCTTCAGAGAATACAAGAAAATTGTTGACATGGCCAAGTTTACGGAGGAAAAGATTAAAAGATTAAAGAGTAGAGCAAGGCGTTTGACTAATCGAATAGAGCAAATTGAGCCCTCCGGTTGGAAGGAGTTCTTGCAGATCAGCAATGTCATACACGAGACTCGAGCATTGGATATCAATACCCATGTCATATTTCCACTTGGTGTAACAGCAGCTGCCATTCGGGGAGAAAACGAACTCTGGCTTGCCATGGTTCTTCGAAACAAAATCTTAATAGGTTTGAAGCCCCCAGAACTTGCTGCCGTCTGCGCAAGTTTAGTATCCGAAGGGATCAAAGTTCGTCCTTGGAAGAACAACAGCTACATATTCGAACCTTCGAGTAAAGTAGTCGACGTGGTCAGCTTCTTGGATGATCAGAGAAGCTCTTTTCTTGAGCTTCAAGAAAAACATGGGGTAAATAAACCTTGCTATTTGGACACACAGTTTTCAGGTATGGTTGAAGCCTGGGTTTCTGGGTTGACTTGGAGGGAAATCATGATGGACTGCGCGATGGACGAGGGTGATTTAGCCCGCCTCCTACGGCGAACCATCGATCTGCTGGCGCAAATTCCCAAGTTGCCGGATATTGATCCGCTACTGCAGAGTAATGCAAAGACGGCGTCAAATATTATGGACCGTCCTCCAATTAGTGAGCTGGCTGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000373 GO:0000375 GO:0000377 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006810 GO:0007275 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009892 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010494 GO:0010496 GO:0010497 GO:0010501 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035770 GO:0036464 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0051179 GO:0051234 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C

Protein Analysis

1176

Amino Acids

132.43

Weight (kDa)

5.67

Isoelectric Point (pI)

51.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	165 - 321	4.3e-28	DEAD/DEAH box helicase
ResIII	PF04851	167 - 317	1.1e-10	Type III restriction enzyme, res subunit
DEXQc_Suv3	PF22527	183 - 316	1.1e-07	DEXQ-box helicase domain of Suv3
SH3_ISE2	PF25446	739 - 917	4.9e-87	ISE2, SH3 domain
DSHCT	PF08148	1004 - 1170	3.8e-33	DSHCT (NUC185) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013378)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70070
fragaria_vesca	FvH4_4g35290 FvH4_4g35290 FvH4_4g35290
malus_domestica	MD16G1083800.v1.1
prunus_persica	Prupe.1G262200_v2.0.a1
pyrus_communis	pycom16g07280
rosa_chinensis	RchiOBHm_Chr4g0444891
rosa_laevigata	RLG00000005791
rosa_multiflora	Rmu_sc0006845.1_g000003 Rmu_sc0013783.1_g000003
rosa_roxburghii	Rroxscaffold_5G00385390
rosa_rugosa	Rorug04G0355800
rosa_samantha	Rh4AG417600 Rh4BG429400 Rh4CG444100 Rh4DG425300
rosa_wichuraiana	Rw4G035790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2517
Acc16I	TGCGCA	2 cut(s)	773, 3133
Acc36I	ACCTGC	1 cut(s)	1793
AccB1I	GGYRCC	1 cut(s)	550
AccI	GTMKAC	3 cut(s)	371, 1540, 3213
AccII	CGCG	7 cut(s)	43, 63, 131, 151, 278, 2815, 3371
AclI	AACGTT	1 cut(s)	2764
AclWI	GGATC	3 cut(s)	2665, 3161, 3446
AcoI	YGGCCR	2 cut(s)	377, 2856
AcsI	RAATTY	3 cut(s)	671, 2043, 3429
AcuI	CTGAAG	8 cut(s)	1125, 1818, 1950, 2055, 2316, 2624, 2812, 2819
AcyI	GRCGYC	2 cut(s)	551, 3482
AdeI	CACNNNGTG	1 cut(s)	3026
AfaI	GTAC	7 cut(s)	242, 287, 883, 1032, 1290, 2285, 2818
AfeI	AGCGCT	1 cut(s)	2547
AfiI	CCNNNNNNNGG	4 cut(s)	1452, 1790, 1944, 2943
AflII	CTTAAG	2 cut(s)	641, 1319
AflIII	ACRYGT	5 cut(s)	306, 777, 870, 1233, 1687
AjiI	CACGTC	4 cut(s)	1196, 1688, 2795, 3217
AjnI	CCWGG	2 cut(s)	2477, 3326
Alw21I	GWGCWC	3 cut(s)	182, 465, 2582
Alw26I	GTCTC	6 cut(s)	674, 1481, 1846, 2054, 2660, 2975
AlwI	GGATC	3 cut(s)	2665, 3161, 3446
AlwNI	CAGNNNCTG	1 cut(s)	3038
Ama87I	CYCGRG	5 cut(s)	175, 320, 1326, 2565, 2985
Aor51HI	AGCGCT	1 cut(s)	2547
AoxI	GGCC	5 cut(s)	377, 645, 703, 2409, 2856
ApeKI	GCWGC	8 cut(s)	587, 1379, 1520, 1619, 2004, 3035, 3038, 3122
ApoI	RAATTY	3 cut(s)	671, 2043, 3429
ArsI	GACNNNNNNTTYG	2 cut(s)	3467, 3499
Asp700I	GAANNNNTTC	6 cut(s)	802, 1454, 1556, 1713, 2301, 2951
AspA2I	CCTAGG	1 cut(s)	100
AspS9I	GGNCC	3 cut(s)	2123, 2184, 3496
AsuC2I	CCSGG	1 cut(s)	2318
AsuHPI	GGTGA	4 cut(s)	138, 703, 1688, 3395
AsuII	TTCGAA	4 cut(s)	2194, 2439, 3082, 3192
AvaI	CYCGRG	5 cut(s)	175, 320, 1326, 2565, 2985
AvaII	GGWCC	3 cut(s)	2123, 2184, 3496
AvrII	CCTAGG	1 cut(s)	100
BaeI	ACNNNNGTAYC	2 cut(s)	503, 536
BalI	TGGCCA	1 cut(s)	2858
BanI	GGYRCC	1 cut(s)	550
BanII	GRGCYC	4 cut(s)	182, 465, 1187, 2937
BauI	CACGAG	2 cut(s)	458, 2978
BbsI	GAAGAC	4 cut(s)	240, 1198, 2061, 2214
Bbv12I	GWGCWC	3 cut(s)	182, 465, 2582
BbvCI	CCTCAGC	1 cut(s)	1664
BbvI	GCAGC	8 cut(s)	574, 1366, 1507, 1606, 2016, 3025, 3047, 3109
BccI	CCATC	7 cut(s)	23, 78, 1160, 2452, 3355, 3367, 3419
BceAI	ACGGC	5 cut(s)	352, 578, 3110, 3419, 3495
BcgI	CGANNNNNNTGC	4 cut(s)	254, 288, 351, 385
BciT130I	CCWGG	2 cut(s)	2479, 3328
BciVI	GTATCC	2 cut(s)	1479, 3154
BclI	TGATCA	1 cut(s)	3235
BcnI	CCSGG	1 cut(s)	2318
BcoDI	GTCTC	6 cut(s)	674, 1481, 1846, 2054, 2660, 2975
BfaI	CTAG	8 cut(s)	101, 609, 1173, 1239, 1641, 1965, 2040, 2549
BfmI	CTRYAG	2 cut(s)	1620, 3461
BfoI	RGCGCY	4 cut(s)	175, 554, 1797, 2549
BfrI	CTTAAG	2 cut(s)	641, 1319
BfuAI	ACCTGC	1 cut(s)	1793
BfuI	GTATCC	2 cut(s)	1479, 3154
BglII	AGATCT	3 cut(s)	765, 2146, 2808
BlnI	CCTAGG	1 cut(s)	100
Bme1390I	CCNGG	3 cut(s)	2318, 2479, 3328
Bme18I	GGWCC	3 cut(s)	2123, 2184, 3496
BmeT110I	CYCGRG	5 cut(s)	175, 320, 1326, 2565, 2985
BmgBI	CACGTC	4 cut(s)	1196, 1688, 2795, 3217
BmgT120I	GGNCC	3 cut(s)	2123, 2184, 3496
BmiI	GGNNCC	2 cut(s)	552, 1457
BmrFI	CCNGG	3 cut(s)	2318, 2479, 3328
BmrI	ACTGGG	1 cut(s)	309
BmsI	GCATC	8 cut(s)	202, 727, 1081, 1366, 1465, 1470, 1667, 2545
BmuI	ACTGGG	1 cut(s)	309
BoxI	GACNNNNGTC	1 cut(s)	1249
BpiI	GAAGAC	4 cut(s)	240, 1198, 2061, 2214
Bpu10I	CCTNAGC	2 cut(s)	741, 1664
Bpu14I	TTCGAA	4 cut(s)	2194, 2439, 3082, 3192
BpuEI	CTTGAG	5 cut(s)	544, 1171, 2348, 2684, 3275
BpuMI	CCSGG	1 cut(s)	2318
Bsa29I	ATCGAT	2 cut(s)	489, 3414
BsaAI	YACGTR	2 cut(s)	829, 2455
BsaBI	GATNNNNATC	1 cut(s)	3450
BsaHI	GRCGYC	2 cut(s)	551, 3482
BsaI	GGTCTC	1 cut(s)	1846
BsaJI	CCNNGG	6 cut(s)	100, 276, 2564, 3072, 3167, 3327
BsaWI	WCCGGW	2 cut(s)	713, 2939
BsaXI	ACNNNNNCTCC	5 cut(s)	26, 377, 407, 2591, 2621
Bsc4I	CCNNNNNNNGG	4 cut(s)	1452, 1790, 1944, 2943
Bse1I	ACTGG	4 cut(s)	304, 1028, 1687, 2693
Bse3DI	GCAATG	2 cut(s)	2014, 2974
Bse8I	GATNNNNATC	1 cut(s)	3450
BseBI	CCWGG	2 cut(s)	2479, 3328
BseCI	ATCGAT	2 cut(s)	489, 3414
BseDI	CCNNGG	6 cut(s)	100, 276, 2564, 3072, 3167, 3327
BseGI	GGATG	8 cut(s)	15, 70, 286, 853, 1376, 1420, 1658, 3238
BseJI	GATNNNNATC	1 cut(s)	3450
BseLI	CCNNNNNNNGG	4 cut(s)	1452, 1790, 1944, 2943
BseMI	GCAATG	2 cut(s)	2014, 2974
BseMII	CTCAG	7 cut(s)	70, 215, 753, 755, 854, 1678, 2171
BseNI	ACTGG	4 cut(s)	304, 1028, 1687, 2693
BseRI	GAGGAG	7 cut(s)	242, 263, 266, 398, 631, 2045, 2258
BseX3I	CGGCCG	1 cut(s)	377
BseXI	GCAGC	8 cut(s)	574, 1366, 1507, 1606, 2016, 3025, 3047, 3109
BseYI	CCCAGC	1 cut(s)	1724
BsgI	GTGCAG	1 cut(s)	1506
Bsh1236I	CGCG	7 cut(s)	43, 63, 131, 151, 278, 2815, 3371
Bsh1285I	CGRYCG	2 cut(s)	380, 2234
BshFI	GGCC	5 cut(s)	379, 647, 705, 2411, 2858
BshNI	GGYRCC	1 cut(s)	550
BshVI	ATCGAT	2 cut(s)	489, 3414
BsiEI	CGRYCG	2 cut(s)	380, 2234
BsiHKAI	GWGCWC	3 cut(s)	182, 465, 2582
BsiHKCI	CYCGRG	5 cut(s)	175, 320, 1326, 2565, 2985
BsiSI	CCGG	5 cut(s)	380, 714, 2318, 2940, 3443
BsiWI	CGTACG	1 cut(s)	2816
BslFI	GGGAC	2 cut(s)	317, 827
BslI	CCNNNNNNNGG	4 cut(s)	1452, 1790, 1944, 2943
BsmAI	GTCTC	6 cut(s)	674, 1481, 1846, 2054, 2660, 2975
BsmFI	GGGAC	2 cut(s)	317, 827
BsnI	GGCC	5 cut(s)	379, 647, 705, 2411, 2858
Bso31I	GGTCTC	1 cut(s)	1846
BsoBI	CYCGRG	5 cut(s)	175, 320, 1326, 2565, 2985
Bsp119I	TTCGAA	4 cut(s)	2194, 2439, 3082, 3192
Bsp1286I	GDGCHC	5 cut(s)	182, 465, 1187, 2582, 2937
Bsp19I	CCATGG	1 cut(s)	3072
BspANI	GGCC	5 cut(s)	379, 647, 705, 2411, 2858
BspCNI	CTCAG	7 cut(s)	69, 214, 754, 754, 855, 1677, 2172
BspDI	ATCGAT	2 cut(s)	489, 3414
BspFNI	CGCG	7 cut(s)	43, 63, 131, 151, 278, 2815, 3371
BspHI	TCATGA	1 cut(s)	3357
BspLI	GGNNCC	2 cut(s)	552, 1457
BspMAI	CTGCAG	2 cut(s)	1624, 3465
BspMI	ACCTGC	1 cut(s)	1793
BspPI	GGATC	3 cut(s)	2665, 3161, 3446
BspQI	GCTCTTC	1 cut(s)	1560
BspT104I	TTCGAA	4 cut(s)	2194, 2439, 3082, 3192
BspT107I	GGYRCC	1 cut(s)	550
BspTI	CTTAAG	2 cut(s)	641, 1319
BspTNI	GGTCTC	1 cut(s)	1846
BsrDI	GCAATG	2 cut(s)	2014, 2974
BsrI	ACTGG	4 cut(s)	304, 1028, 1687, 2693
BssECI	CCNNGG	6 cut(s)	100, 276, 2564, 3072, 3167, 3327
BssNI	GRCGYC	2 cut(s)	551, 3482
BssSI	CACGAG	2 cut(s)	458, 2978
BssT1I	CCWWGG	3 cut(s)	100, 3072, 3167
Bst2BI	CACGAG	2 cut(s)	458, 2978
Bst2UI	CCWGG	2 cut(s)	2479, 3328
Bst6I	CTCTTC	5 cut(s)	219, 240, 1421, 1445, 1560
BstACI	GRCGYC	2 cut(s)	551, 3482
BstAFI	CTTAAG	2 cut(s)	641, 1319
BstAPI	GCANNNNNTGC	2 cut(s)	1529, 2402
BstBAI	YACGTR	2 cut(s)	829, 2455
BstBI	TTCGAA	4 cut(s)	2194, 2439, 3082, 3192
BstDEI	CTNAG	7 cut(s)	56, 201, 741, 762, 863, 1664, 2180
BstDSI	CCRYGG	2 cut(s)	276, 3072
BstENI	CCTNNNNNAGG	2 cut(s)	1450, 1788
BstF5I	GGATG	8 cut(s)	15, 70, 286, 853, 1376, 1420, 1658, 3238
BstFNI	CGCG	7 cut(s)	43, 63, 131, 151, 278, 2815, 3371
BstH2I	RGCGCY	4 cut(s)	175, 554, 1797, 2549
BstMAI	GTCTC	6 cut(s)	674, 1481, 1846, 2054, 2660, 2975
BstMCI	CGRYCG	2 cut(s)	380, 2234
BstNI	CCWGG	2 cut(s)	2479, 3328
BstNSI	RCATGY	4 cut(s)	310, 781, 874, 1237
BstPAI	GACNNNNGTC	1 cut(s)	1249
BstSCI	CCNGG	3 cut(s)	2316, 2477, 3326
BstSFI	CTRYAG	2 cut(s)	1620, 3461
BstUI	CGCG	7 cut(s)	43, 63, 131, 151, 278, 2815, 3371
BstV1I	GCAGC	8 cut(s)	574, 1366, 1507, 1606, 2016, 3025, 3047, 3109
BstV2I	GAAGAC	4 cut(s)	240, 1198, 2061, 2214
BstX2I	RGATCY	4 cut(s)	765, 2146, 2657, 2808
BstXI	CCANNNNNNTGG	2 cut(s)	1041, 1347
BstYI	RGATCY	4 cut(s)	765, 2146, 2657, 2808
BstZI	CGGCCG	1 cut(s)	377
Bsu15I	ATCGAT	2 cut(s)	489, 3414
BsuI	GTATCC	2 cut(s)	1479, 3154
BsuRI	GGCC	5 cut(s)	379, 647, 705, 2411, 2858
BsuTUI	ATCGAT	2 cut(s)	489, 3414
BtgI	CCRYGG	2 cut(s)	276, 3072
BtgZI	GCGATG	3 cut(s)	705, 2097, 3386
BtrI	CACGTC	4 cut(s)	1196, 1688, 2795, 3217
BtsCI	GGATG	8 cut(s)	15, 70, 286, 853, 1376, 1420, 1658, 3238
BtsI	GCAGTG	2 cut(s)	568, 2412
BtsIMutI	CAGTG	8 cut(s)	568, 759, 891, 1247, 1680, 1926, 2063, 2412
BveI	ACCTGC	1 cut(s)	1793
CaiI	CAGNNNCTG	1 cut(s)	3038
CciI	TCATGA	1 cut(s)	3357
Cfr13I	GGNCC	3 cut(s)	2123, 2184, 3496
Cfr42I	CCGCGG	1 cut(s)	279
ClaI	ATCGAT	2 cut(s)	489, 3414
CseI	GACGC	3 cut(s)	368, 1013, 3471
Csp6I	GTAC	7 cut(s)	241, 286, 882, 1031, 1289, 2284, 2817
CviQI	GTAC	7 cut(s)	241, 286, 882, 1031, 1289, 2284, 2817
DdeI	CTNAG	7 cut(s)	56, 201, 741, 762, 863, 1664, 2180
DinI	GGCGCC	1 cut(s)	552
DraIII	CACNNNGTG	1 cut(s)	3026
EaeI	YGGCCR	2 cut(s)	377, 2856
EagI	CGGCCG	1 cut(s)	377
Eam1104I	CTCTTC	5 cut(s)	219, 240, 1421, 1445, 1560
EarI	CTCTTC	5 cut(s)	219, 240, 1421, 1445, 1560
EciI	GGCGGA	2 cut(s)	172, 628
Ecl136II	GAGCTC	2 cut(s)	180, 463
EclXI	CGGCCG	1 cut(s)	377
Eco130I	CCWWGG	3 cut(s)	100, 3072, 3167
Eco147I	AGGCCT	1 cut(s)	647
Eco24I	GRGCYC	4 cut(s)	182, 465, 1187, 2937
Eco31I	GGTCTC	1 cut(s)	1846
Eco32I	GATATC	2 cut(s)	1402, 2998
Eco47I	GGWCC	3 cut(s)	2123, 2184, 3496
Eco47III	AGCGCT	1 cut(s)	2547
Eco52I	CGGCCG	1 cut(s)	377
Eco53kI	GAGCTC	2 cut(s)	180, 463
Eco57I	CTGAAG	8 cut(s)	1125, 1818, 1950, 2055, 2316, 2624, 2812, 2819
Eco88I	CYCGRG	5 cut(s)	175, 320, 1326, 2565, 2985
EcoICRI	GAGCTC	2 cut(s)	180, 463
EcoNI	CCTNNNNNAGG	2 cut(s)	1450, 1788
EcoRI	GAATTC	1 cut(s)	2043
EcoRII	CCWGG	2 cut(s)	2477, 3326
EcoRV	GATATC	2 cut(s)	1402, 2998
EcoT14I	CCWWGG	3 cut(s)	100, 3072, 3167
EcoT38I	GRGCYC	4 cut(s)	182, 465, 1187, 2937
EgeI	GGCGCC	1 cut(s)	552
EheI	GGCGCC	1 cut(s)	552
ErhI	CCWWGG	3 cut(s)	100, 3072, 3167
FalI	AAGNNNNNCTT	8 cut(s)	1583, 1615, 2019, 2051, 2646, 2678, 2939, 2971
FaqI	GGGAC	2 cut(s)	317, 827
FauI	CCCGC	5 cut(s)	89, 136, 199, 1572, 3401
FauNDI	CATATG	2 cut(s)	932, 1861
FbaI	TGATCA	1 cut(s)	3235
FblI	GTMKAC	3 cut(s)	371, 1540, 3213
FokI	GGATG	8 cut(s)	2, 57, 293, 860, 1383, 1427, 1645, 3245
FriOI	GRGCYC	4 cut(s)	182, 465, 1187, 2937
FspBI	CTAG	8 cut(s)	101, 609, 1173, 1239, 1641, 1965, 2040, 2549
FspI	TGCGCA	2 cut(s)	773, 3133
GsaI	CCCAGC	1 cut(s)	1728
HaeII	RGCGCY	4 cut(s)	175, 554, 1797, 2549
HaeIII	GGCC	5 cut(s)	379, 647, 705, 2411, 2858
HapII	CCGG	5 cut(s)	380, 714, 2318, 2940, 3443
HgaI	GACGC	3 cut(s)	368, 1013, 3471
Hin1I	GRCGYC	2 cut(s)	551, 3482
HincII	GTYRAC	6 cut(s)	372, 2341, 2350, 2851, 3214, 3342
HindII	GTYRAC	6 cut(s)	372, 2341, 2350, 2851, 3214, 3342
HindIII	AAGCTT	1 cut(s)	2615
HpaII	CCGG	5 cut(s)	380, 714, 2318, 2940, 3443
HphI	GGTGA	4 cut(s)	138, 703, 1688, 3395
HpyF3I	CTNAG	7 cut(s)	56, 201, 741, 762, 863, 1664, 2180
Hsp92I	GRCGYC	2 cut(s)	551, 3482
KasI	GGCGCC	1 cut(s)	550
Ksp22I	TGATCA	1 cut(s)	3235
KspI	CCGCGG	1 cut(s)	279
LguI	GCTCTTC	1 cut(s)	1560
LmnI	GCTCC	4 cut(s)	116, 185, 2032, 2599
Lsp1109I	GCAGC	8 cut(s)	574, 1366, 1507, 1606, 2016, 3025, 3047, 3109
LweI	GCATC	8 cut(s)	202, 727, 1081, 1366, 1465, 1470, 1667, 2545
MaeI	CTAG	8 cut(s)	101, 609, 1173, 1239, 1641, 1965, 2040, 2549
MaeIII	GTNAC	8 cut(s)	295, 866, 1009, 1229, 1676, 1897, 2765, 3028
MfeI	CAATTG	1 cut(s)	2091
MflI	RGATCY	4 cut(s)	765, 2146, 2657, 2808
MhlI	GDGCHC	5 cut(s)	182, 465, 1187, 2582, 2937
MlsI	TGGCCA	1 cut(s)	2858
MluNI	TGGCCA	1 cut(s)	2858
Mly113I	GGCGCC	1 cut(s)	551
MlyI	GAGTC	7 cut(s)	168, 312, 367, 628, 1318, 2633, 2977
MmeI	TCCRAC	5 cut(s)	54, 164, 291, 956, 2924
Mox20I	TGGCCA	1 cut(s)	2858
MroXI	GAANNNNTTC	6 cut(s)	802, 1454, 1556, 1713, 2301, 2951
MscI	TGGCCA	1 cut(s)	2858
MslI	CAYNNNNRTG	1 cut(s)	831
Msp20I	TGGCCA	1 cut(s)	2858
MspA1I	CMGCKG	3 cut(s)	278, 1579, 3038
MspCI	CTTAAG	2 cut(s)	641, 1319
MspI	CCGG	5 cut(s)	380, 714, 2318, 2940, 3443
MspR9I	CCNGG	3 cut(s)	2318, 2479, 3328
MunI	CAATTG	1 cut(s)	2091
MvaI	CCWGG	2 cut(s)	2479, 3328
MvnI	CGCG	7 cut(s)	43, 63, 131, 151, 278, 2815, 3371
NarI	GGCGCC	1 cut(s)	551
NciI	CCSGG	1 cut(s)	2318
NcoI	CCATGG	1 cut(s)	3072
NdeI	CATATG	2 cut(s)	932, 1861
NlaIV	GGNNCC	2 cut(s)	552, 1457
NmeAIII	GCCGAG	3 cut(s)	355, 2613, 2647
NmuCI	GTSAC	2 cut(s)	866, 1676
NsbI	TGCGCA	2 cut(s)	773, 3133
NspI	RCATGY	4 cut(s)	310, 781, 874, 1237
NspV	TTCGAA	4 cut(s)	2194, 2439, 3082, 3192
PaeR7I	CTCGAG	2 cut(s)	320, 2985
PagI	TCATGA	1 cut(s)	3357
PceI	AGGCCT	1 cut(s)	647
PciI	ACATGT	4 cut(s)	306, 777, 870, 1233
PciSI	GCTCTTC	1 cut(s)	1560
PcsI	WCGNNNNNNNCGW	4 cut(s)	267, 327, 554, 673
PdmI	GAANNNNTTC	6 cut(s)	802, 1454, 1556, 1713, 2301, 2951
Pfl23II	CGTACG	1 cut(s)	2816
PflFI	GACNNNGTC	6 cut(s)	698, 2065, 2345, 2671, 3218, 3481
PleI	GAGTC	7 cut(s)	167, 312, 367, 628, 1318, 2632, 2977
PluTI	GGCGCC	1 cut(s)	554
PpsI	GAGTC	7 cut(s)	167, 312, 367, 628, 1318, 2632, 2977
Ppu21I	YACGTR	2 cut(s)	829, 2455
PscI	ACATGT	4 cut(s)	306, 777, 870, 1233
PshAI	GACNNNNGTC	1 cut(s)	1249
PsiI	TTATAA	1 cut(s)	2517
Psp124BI	GAGCTC	2 cut(s)	182, 465
Psp1406I	AACGTT	1 cut(s)	2764
Psp6I	CCWGG	2 cut(s)	2477, 3326
PspFI	CCCAGC	1 cut(s)	1724
PspGI	CCWGG	2 cut(s)	2477, 3326
PspLI	CGTACG	1 cut(s)	2816
PspN4I	GGNNCC	2 cut(s)	552, 1457
PspPI	GGNCC	3 cut(s)	2123, 2184, 3496
PspXI	VCTCGAGB	2 cut(s)	320, 2985
PsrI	GAACNNNNNNTAC	4 cut(s)	1758, 1790, 3167, 3199
PstI	CTGCAG	2 cut(s)	1624, 3465
PstNI	CAGNNNCTG	1 cut(s)	3038
PsuI	RGATCY	4 cut(s)	765, 2146, 2657, 2808
PsyI	GACNNNGTC	6 cut(s)	698, 2065, 2345, 2671, 3218, 3481
PvuII	CAGCTG	1 cut(s)	3038
RsaI	GTAC	7 cut(s)	242, 287, 883, 1032, 1290, 2285, 2818
RsaNI	GTAC	7 cut(s)	241, 286, 882, 1031, 1289, 2284, 2817
RseI	CAYNNNNRTG	1 cut(s)	831
SacI	GAGCTC	2 cut(s)	182, 465
SacII	CCGCGG	1 cut(s)	279
SalI	GTCGAC	2 cut(s)	370, 3212
SapI	GCTCTTC	1 cut(s)	1560
Sau96I	GGNCC	3 cut(s)	2123, 2184, 3496
SchI	GAGTC	7 cut(s)	168, 312, 367, 628, 1318, 2633, 2977
ScrFI	CCNGG	3 cut(s)	2318, 2479, 3328
SduI	GDGCHC	5 cut(s)	182, 465, 1187, 2582, 2937
SfaNI	GCATC	8 cut(s)	202, 727, 1081, 1366, 1465, 1470, 1667, 2545
SfcI	CTRYAG	2 cut(s)	1620, 3461
SfoI	GGCGCC	1 cut(s)	552
Sfr274I	CTCGAG	2 cut(s)	320, 2985
Sfr303I	CCGCGG	1 cut(s)	279
SfuI	TTCGAA	4 cut(s)	2194, 2439, 3082, 3192
SgrBI	CCGCGG	1 cut(s)	279
SinI	GGWCC	3 cut(s)	2123, 2184, 3496
SlaI	CTCGAG	2 cut(s)	320, 2985
SmiMI	CAYNNNNRTG	1 cut(s)	831
SmlI	CTYRAG	9 cut(s)	320, 523, 641, 1186, 1319, 2363, 2663, 2985, 3254
SmoI	CTYRAG	9 cut(s)	320, 523, 641, 1186, 1319, 2363, 2663, 2985, 3254
SseBI	AGGCCT	1 cut(s)	647
SspDI	GGCGCC	1 cut(s)	550
SspI	AATATT	1 cut(s)	3490
SspMI	CTAG	8 cut(s)	101, 609, 1173, 1239, 1641, 1965, 2040, 2549
SstI	GAGCTC	2 cut(s)	182, 465
StuI	AGGCCT	1 cut(s)	647
StyD4I	CCNGG	3 cut(s)	2316, 2477, 3326
StyI	CCWWGG	3 cut(s)	100, 3072, 3167
TaqII	GACCGA	1 cut(s)	2201
TatI	WGTACW	1 cut(s)	2283
TauI	GCSGC	5 cut(s)	63, 278, 420, 593, 2024
TscAI	CASTG	8 cut(s)	568, 766, 891, 1254, 1687, 1933, 2070, 2412
TseFI	GTSAC	2 cut(s)	866, 1676
TseI	GCWGC	8 cut(s)	587, 1379, 1520, 1619, 2004, 3035, 3038, 3122
Tsp45I	GTSAC	2 cut(s)	866, 1676
TspGWI	ACGGA	5 cut(s)	330, 831, 2271, 2612, 2882
TspRI	CASTG	8 cut(s)	568, 766, 891, 1254, 1687, 1933, 2070, 2412
Tth111I	GACNNNGTC	6 cut(s)	698, 2065, 2345, 2671, 3218, 3481
Vha464I	CTTAAG	2 cut(s)	641, 1319
VpaK11BI	GGWCC	3 cut(s)	2123, 2184, 3496
XagI	CCTNNNNNAGG	2 cut(s)	1450, 1788
XapI	RAATTY	3 cut(s)	671, 2043, 3429
XbaI	TCTAGA	2 cut(s)	1172, 1238
XceI	RCATGY	4 cut(s)	310, 781, 874, 1237
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3418
XhoI	CTCGAG	2 cut(s)	320, 2985
XmaJI	CCTAGG	1 cut(s)	100
XmiI	GTMKAC	3 cut(s)	371, 1540, 3213
XmnI	GAANNNNTTC	6 cut(s)	802, 1454, 1556, 1713, 2301, 2951
XspI	CTAG	8 cut(s)	101, 609, 1173, 1239, 1641, 1965, 2040, 2549

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.