FvH4_5g02080

4-coumarate--CoA ligase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008550
Physical Location & Seq
Reverse (-)
1298543 .. 1301267
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g02080.t5

Sequence Viewer

Length: 1575 bp
ATGGAAAACTCGACCTTGACCGGTTTGTTGAAGAAGGTGGCGTCGGAGTTCCCGAACCGCCGCGCTATTTCCGTTTCCGGCAAATTCGACTTGACCCACGCCCGATTACATGAGCTCGTGGAGTACGCTGCCTCACAATTACTCGCCGCCGGCGTCGCCCCTCACGACAGCATCGCTCTCACCTTCCCCAACTCCATTGAGTATGTGGTGATGTTCTTGGCCGTGATACGGTGCCGAGCCACCGCCGCGCCGCTGAACGCGGCGTACACGGCGGAGGAGTTCGAGTTTTATCTCTCCGACTCCGAGTCGAAGCTTCTGATTACTTCGGAGAAGCCGATTCAGTCCGCTGTGAGCGCCGCTACCAAGCTGAGCATACCTTACGTGACGGCGGCGTTGTCCGAGGGTGGCGGTAACGTTTGTCTGTCTTCCGCCACTGTGGGCGAGCCAAGTTGCGATTCAATTTCGAAATTCGTTAACGACCCGTCCGACGTGGCGCTCTTCCTCCACACGTCCGGGACCACCAGCCGGCCGAAGGGAGTTCCTCTGACGCAGCTCAATCTGGCCTCGTCGGTACGGAACATCCGATCAGTCTATAGACTCACGGAGTCGGACTCGACGGTGATCGTTCTGCCGTTGTTTCACGTGCACGGATTAATCGCGGGGTTGTTGAGTTCATTTACCGCCGGCGCCGCCGTGACGCTGCCGGCGGCGGGAAGATTCTCCGCCTCGACTTTCTGGGCCGACATGCTCAAATACAACGCCACGTGGTACACGGCGGTTCCCACCATCCACCAGATCATTCTCGACCGTCACGCCAACAAACCCGAACCGGCCTACCCGAAGCTGCGGTTTATCCGGAGCTGCAGCGCGTCGCTGGCACCATCGATTTTGGCGCGTCTGGAGGAGTCGTTCGGCGCGCCGGTTTTGGAGGCGTACGCGATGACGGAGGCGACCCATTTGATGTGTTCGAACCCGTTACCCGAAGACGGCGCGCACAAACCCGGGTCCGTCGGGAAACCCGTGGGCCAGGAGCTGGCCATTTTGAACGAAAACGGCGTCGTTCAGCCAAGTGACGTCAGTGGTGAGGTCTGCATTAGAGGACCAAATGTGACAAAAGGGTATAAGAACAACCCGGAAGCTAACAAAGCTGCCTTCACATTCGGGTGGTTCCACACCGGAGATGTAGGGTTTTTGGACTCCGACGGGTACTTACACCTTGTCGGTCGGATCAAGGAGCTCATTAACCGTGGAGGAGAGAAGATATCACCTATTGAAGTTGACGCAGTGCTATTGTCACACCCCGAGATCTGTCAGGCGGTTTGTTTCGGAGTGCCTGATGATAAATATGGTGAAGAGATTAACTGTGCTATAATTCCAAGAGAAGGCTCGAGCATAGACGAGGCGGAAGTGATGCGCTTCTGCAAGAAGAACCTTGCAGCTTTCAAGGTGCCGAAGAAGGTGTTCATCACTGACTCTGTTCCGAAAACTGCAACGGGGAAGATCCAACGTCGCATTGTGGCAGAGCATTTCCTTGCTCAAATCTCCACTGCCAAAGTTCCCAAGTTTGGCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00630 ko01100 map00630 map01100
Pfam Domains
Protein Families
AMP-binding AMP-binding_C Pkinase

Protein Analysis

525

Amino Acids

56.28

Weight (kDa)

6.55

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 11 - 374 5.3e-82 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1077
AccB1I GGYRCC 4 cut(s) 231, 686, 877, 1447
AccB7I CCANNNNNTGG 1 cut(s) 1033
AccII CGCG 9 cut(s) 63, 248, 260, 659, 869, 895, 917, 938, 992
AccIII TCCGGA 1 cut(s) 855
AclI AACGTT 1 cut(s) 414
AclWI GGATC 2 cut(s) 1235, 1495
AcoI YGGCCR 3 cut(s) 219, 527, 1035
AcsI RAATTY 2 cut(s) 83, 467
AcvI CACGTG 2 cut(s) 643, 765
AcyI GRCGYC 5 cut(s) 41, 153, 687, 1056, 1074
AfaI GTAC 6 cut(s) 125, 266, 573, 770, 935, 1208
AfiI CCNNNNNNNGG 8 cut(s) 228, 525, 532, 710, 986, 1033, 1382, 1565
AflIII ACRYGT 1 cut(s) 507
AgeI ACCGGT 1 cut(s) 20
AgsI TTSAA 5 cut(s) 31, 459, 1045, 1274, 1444
AhdI GACNNNNNGTC 1 cut(s) 304
AjiI CACGTC 2 cut(s) 490, 510
AjnI CCWGG 1 cut(s) 1026
AjuI GAANNNNNNNTTGG 2 cut(s) 439, 471
AloI GAACNNNNNNTCC 2 cut(s) 893, 925
Alw21I GWGCWC 3 cut(s) 117, 648, 1239
Alw44I GTGCAC 1 cut(s) 644
AlwI GGATC 2 cut(s) 1235, 1495
AlwNI CAGNNNCTG 1 cut(s) 1033
Ama87I CYCGRG 3 cut(s) 1001, 1301, 1387
Aor13HI TCCGGA 1 cut(s) 855
AoxI GGCC 7 cut(s) 219, 527, 561, 738, 831, 1024, 1035
ApaLI GTGCAC 1 cut(s) 644
ApeKI GCWGC 8 cut(s) 128, 550, 700, 844, 861, 864, 1148, 1436
ApoI RAATTY 2 cut(s) 83, 467
ArsI GACNNNNNNTTYG 2 cut(s) 1041, 1073
AscI GGCGCGCC 1 cut(s) 915
AseI ATTAAT 1 cut(s) 653
AsiGI ACCGGT 1 cut(s) 20
Asp700I GAANNNNTTC 1 cut(s) 1460
AspS9I GGNCC 5 cut(s) 516, 738, 1005, 1024, 1100
AsuC2I CCSGG 4 cut(s) 514, 1002, 1003, 1133
AsuHPI GGTGA 6 cut(s) 172, 220, 631, 1094, 1257, 1361
AsuII TTCGAA 2 cut(s) 464, 968
AvaI CYCGRG 3 cut(s) 1001, 1301, 1387
AvaII GGWCC 3 cut(s) 516, 1005, 1100
BaeGI GKGCMC 1 cut(s) 648
BalI TGGCCA 1 cut(s) 1037
BanI GGYRCC 4 cut(s) 231, 686, 877, 1447
BanII GRGCYC 2 cut(s) 117, 1239
BauI CACGAG 1 cut(s) 116
BbrPI CACGTG 2 cut(s) 643, 765
BbsI GAAGAC 2 cut(s) 417, 990
Bbv12I GWGCWC 3 cut(s) 117, 648, 1239
BbvI GCAGC 8 cut(s) 115, 562, 687, 831, 848, 876, 1135, 1448
BccI CCATC 2 cut(s) 794, 889
BceAI ACGGC 8 cut(s) 206, 285, 402, 616, 677, 789, 1003, 1069
BciT130I CCWGG 1 cut(s) 1028
BcnI CCSGG 4 cut(s) 514, 1002, 1003, 1133
BfmI CTRYAG 2 cut(s) 592, 862
BfoI RGCGCY 4 cut(s) 357, 497, 690, 1572
BglII AGATCT 1 cut(s) 1305
BlpI GCTNAGC 1 cut(s) 368
Bme1390I CCNGG 5 cut(s) 514, 1002, 1003, 1028, 1133
Bme18I GGWCC 3 cut(s) 516, 1005, 1100
BmeRI GACNNNNNGTC 1 cut(s) 304
BmeT110I CYCGRG 3 cut(s) 1001, 1301, 1387
BmgBI CACGTC 2 cut(s) 490, 510
BmgT120I GGNCC 5 cut(s) 516, 738, 1005, 1024, 1100
BmiI GGNNCC 8 cut(s) 233, 517, 688, 780, 879, 1006, 1169, 1449
BmrFI CCNGG 5 cut(s) 514, 1002, 1003, 1028, 1133
BmsI GCATC 2 cut(s) 180, 1401
BpiI GAAGAC 2 cut(s) 417, 990
BplI GAGNNNNNCTC 2 cut(s) 596, 628
BpmI CTGGAG 1 cut(s) 920
Bpu1102I GCTNAGC 1 cut(s) 368
Bpu14I TTCGAA 2 cut(s) 464, 968
BpuMI CCSGG 4 cut(s) 514, 1002, 1003, 1133
Bsa29I ATCGAT 1 cut(s) 884
BsaAI YACGTR 3 cut(s) 382, 643, 765
BsaHI GRCGYC 5 cut(s) 41, 153, 687, 1056, 1074
BsaJI CCNNGG 4 cut(s) 399, 1001, 1020, 1246
BsaWI WCCGGW 3 cut(s) 20, 855, 1175
BsaXI ACNNNNNCTCC 4 cut(s) 269, 299, 893, 923
Bsc4I CCNNNNNNNGG 8 cut(s) 228, 525, 532, 710, 986, 1033, 1382, 1565
Bse118I RCCGGY 7 cut(s) 20, 149, 525, 683, 703, 829, 919
BseAI TCCGGA 1 cut(s) 855
BseBI CCWGG 1 cut(s) 1028
BseCI ATCGAT 1 cut(s) 884
BseDI CCNNGG 4 cut(s) 399, 1001, 1020, 1246
BseGI GGATG 2 cut(s) 579, 786
BseLI CCNNNNNNNGG 8 cut(s) 228, 525, 532, 710, 986, 1033, 1382, 1565
BseMII CTCAG 1 cut(s) 359
BsePI GCGCGC 2 cut(s) 915, 990
BseRI GAGGAG 3 cut(s) 290, 917, 1266
BseSI GKGCMC 1 cut(s) 648
BseX3I CGGCCG 1 cut(s) 527
BseXI GCAGC 8 cut(s) 115, 562, 687, 831, 848, 876, 1135, 1448
Bsh1236I CGCG 9 cut(s) 63, 248, 260, 659, 869, 895, 917, 938, 992
Bsh1285I CGRYCG 3 cut(s) 530, 808, 1225
BshFI GGCC 7 cut(s) 221, 529, 563, 740, 833, 1026, 1037
BshNI GGYRCC 4 cut(s) 231, 686, 877, 1447
BshTI ACCGGT 1 cut(s) 20
BshVI ATCGAT 1 cut(s) 884
BsiEI CGRYCG 3 cut(s) 530, 808, 1225
BsiHKAI GWGCWC 3 cut(s) 117, 648, 1239
BsiHKCI CYCGRG 3 cut(s) 1001, 1301, 1387
BsiWI CGTACG 1 cut(s) 933
BslFI GGGAC 1 cut(s) 529
BslI CCNNNNNNNGG 8 cut(s) 228, 525, 532, 710, 986, 1033, 1382, 1565
BsmFI GGGAC 1 cut(s) 529
BsnI GGCC 7 cut(s) 221, 529, 563, 740, 833, 1026, 1037
BsoBI CYCGRG 3 cut(s) 1001, 1301, 1387
Bsp119I TTCGAA 2 cut(s) 464, 968
Bsp1286I GDGCHC 3 cut(s) 117, 648, 1239
Bsp13I TCCGGA 1 cut(s) 855
Bsp143I GATC 6 cut(s) 584, 621, 795, 1227, 1305, 1500
Bsp1720I GCTNAGC 1 cut(s) 368
BspANI GGCC 7 cut(s) 221, 529, 563, 740, 833, 1026, 1037
BspCNI CTCAG 1 cut(s) 360
BspDI ATCGAT 1 cut(s) 884
BspEI TCCGGA 1 cut(s) 855
BspFNI CGCG 9 cut(s) 63, 248, 260, 659, 869, 895, 917, 938, 992
BspLI GGNNCC 8 cut(s) 233, 517, 688, 780, 879, 1006, 1169, 1449
BspMAI CTGCAG 1 cut(s) 866
BspPI GGATC 2 cut(s) 1235, 1495
BspQI GCTCTTC 1 cut(s) 503
BspT104I TTCGAA 2 cut(s) 464, 968
BspT107I GGYRCC 4 cut(s) 231, 686, 877, 1447
BsrFI RCCGGY 7 cut(s) 20, 149, 525, 683, 703, 829, 919
BssAI RCCGGY 7 cut(s) 20, 149, 525, 683, 703, 829, 919
BssECI CCNNGG 4 cut(s) 399, 1001, 1020, 1246
BssHII GCGCGC 2 cut(s) 915, 990
BssMI GATC 6 cut(s) 584, 621, 795, 1227, 1305, 1500
BssNI GRCGYC 5 cut(s) 41, 153, 687, 1056, 1074
BssSI CACGAG 1 cut(s) 116
Bst2BI CACGAG 1 cut(s) 116
Bst2UI CCWGG 1 cut(s) 1028
Bst4CI ACNGT 6 cut(s) 231, 436, 619, 809, 1247, 1364
Bst6I CTCTTC 2 cut(s) 503, 1347
BstACI GRCGYC 5 cut(s) 41, 153, 687, 1056, 1074
BstBAI YACGTR 3 cut(s) 382, 643, 765
BstBI TTCGAA 2 cut(s) 464, 968
BstC8I GCNNGC 9 cut(s) 151, 443, 527, 685, 705, 876, 917, 992, 1035
BstDEI CTNAG 1 cut(s) 368
BstDSI CCRYGG 2 cut(s) 1020, 1246
BstF5I GGATG 2 cut(s) 579, 786
BstFNI CGCG 9 cut(s) 63, 248, 260, 659, 869, 895, 917, 938, 992
BstH2I RGCGCY 4 cut(s) 357, 497, 690, 1572
BstKTI GATC 6 cut(s) 587, 624, 798, 1230, 1308, 1503
BstMBI GATC 6 cut(s) 584, 621, 795, 1227, 1305, 1500
BstMCI CGRYCG 3 cut(s) 530, 808, 1225
BstMWI GCNNNNNNNGC 7 cut(s) 155, 245, 269, 353, 689, 875, 1145
BstNI CCWGG 1 cut(s) 1028
BstNSI RCATGY 1 cut(s) 748
BstSCI CCNGG 5 cut(s) 512, 1000, 1001, 1026, 1131
BstSFI CTRYAG 2 cut(s) 592, 862
BstSLI GKGCMC 1 cut(s) 648
BstUI CGCG 9 cut(s) 63, 248, 260, 659, 869, 895, 917, 938, 992
BstV1I GCAGC 8 cut(s) 115, 562, 687, 831, 848, 876, 1135, 1448
BstV2I GAAGAC 2 cut(s) 417, 990
BstX2I RGATCY 2 cut(s) 1305, 1500
BstYI RGATCY 2 cut(s) 1305, 1500
BstZI CGGCCG 1 cut(s) 527
Bsu15I ATCGAT 1 cut(s) 884
BsuRI GGCC 7 cut(s) 221, 529, 563, 740, 833, 1026, 1037
BsuTUI ATCGAT 1 cut(s) 884
BtgI CCRYGG 2 cut(s) 1020, 1246
BtgZI GCGATG 2 cut(s) 157, 953
BtrI CACGTC 2 cut(s) 490, 510
BtsCI GGATG 2 cut(s) 579, 786
BtsI GCAGTG 2 cut(s) 1290, 1545
BtsIMutI CAGTG 5 cut(s) 432, 1084, 1290, 1467, 1545
Cac8I GCNNGC 9 cut(s) 151, 443, 527, 685, 705, 876, 917, 992, 1035
CaiI CAGNNNCTG 1 cut(s) 1033
Cfr10I RCCGGY 7 cut(s) 20, 149, 525, 683, 703, 829, 919
Cfr13I GGNCC 5 cut(s) 516, 738, 1005, 1024, 1100
Cfr9I CCCGGG 1 cut(s) 1001
ClaI ATCGAT 1 cut(s) 884
CseI GACGC 8 cut(s) 30, 142, 556, 706, 858, 884, 1045, 1289
Csp6I GTAC 6 cut(s) 124, 265, 572, 769, 934, 1207
CspAI ACCGGT 1 cut(s) 20
CviAII CATG 2 cut(s) 110, 745
CviQI GTAC 6 cut(s) 124, 265, 572, 769, 934, 1207
DdeI CTNAG 1 cut(s) 368
DinI GGCGCC 1 cut(s) 688
DpnI GATC 6 cut(s) 586, 623, 797, 1229, 1307, 1502
DpnII GATC 6 cut(s) 584, 621, 795, 1227, 1305, 1500
DriI GACNNNNNGTC 1 cut(s) 304
EaeI YGGCCR 3 cut(s) 219, 527, 1035
EagI CGGCCG 1 cut(s) 527
Eam1104I CTCTTC 2 cut(s) 503, 1347
Eam1105I GACNNNNNGTC 1 cut(s) 304
EarI CTCTTC 2 cut(s) 503, 1347
EciI GGCGGA 4 cut(s) 287, 418, 712, 1418
Ecl136II GAGCTC 2 cut(s) 115, 1237
EclXI CGGCCG 1 cut(s) 527
Eco24I GRGCYC 2 cut(s) 117, 1239
Eco32I GATATC 1 cut(s) 1263
Eco47I GGWCC 3 cut(s) 516, 1005, 1100
Eco52I CGGCCG 1 cut(s) 527
Eco53kI GAGCTC 2 cut(s) 115, 1237
Eco72I CACGTG 2 cut(s) 643, 765
Eco88I CYCGRG 3 cut(s) 1001, 1301, 1387
EcoICRI GAGCTC 2 cut(s) 115, 1237
EcoRII CCWGG 1 cut(s) 1026
EcoRV GATATC 1 cut(s) 1263
EcoT38I GRGCYC 2 cut(s) 117, 1239
EgeI GGCGCC 1 cut(s) 688
EheI GGCGCC 1 cut(s) 688
FaeI CATG 2 cut(s) 113, 748
FaiI YATR 9 cut(s) 111, 204, 374, 594, 746, 1122, 1347, 1370, 1394
FaqI GGGAC 1 cut(s) 529
FatI CATG 2 cut(s) 109, 744
FauI CCCGC 2 cut(s) 652, 703
FokI GGATG 2 cut(s) 566, 773
FriOI GRGCYC 2 cut(s) 117, 1239
GsuI CTGGAG 1 cut(s) 920
HaeII RGCGCY 4 cut(s) 357, 497, 690, 1572
HaeIII GGCC 7 cut(s) 221, 529, 563, 740, 833, 1026, 1037
HgaI GACGC 8 cut(s) 30, 142, 556, 706, 858, 884, 1045, 1289
Hin1I GRCGYC 5 cut(s) 41, 153, 687, 1056, 1074
Hin1II CATG 2 cut(s) 113, 748
HincII GTYRAC 2 cut(s) 475, 1279
HindII GTYRAC 2 cut(s) 475, 1279
HindIII AAGCTT 1 cut(s) 311
HpaI GTTAAC 1 cut(s) 475
HphI GGTGA 6 cut(s) 172, 220, 631, 1094, 1257, 1361
Hpy166II GTNNAC 5 cut(s) 267, 475, 646, 771, 1279
Hpy188III TCNNGA 6 cut(s) 52, 164, 803, 856, 899, 1012
Hpy8I GTNNAC 5 cut(s) 267, 475, 646, 771, 1279
HpyAV CCTTC 6 cut(s) 28, 193, 526, 1162, 1376, 1450
HpyCH4III ACNGT 6 cut(s) 231, 436, 619, 809, 1247, 1364
HpyCH4IV ACGT 8 cut(s) 381, 414, 489, 509, 642, 764, 1074, 1507
HpyCH4V TGCA 6 cut(s) 646, 864, 1092, 1422, 1436, 1490
HpyF10VI GCNNNNNNNGC 7 cut(s) 155, 245, 269, 353, 689, 875, 1145
HpyF3I CTNAG 1 cut(s) 368
HpySE526I ACGT 8 cut(s) 381, 414, 489, 509, 642, 764, 1074, 1507
Hsp92I GRCGYC 5 cut(s) 41, 153, 687, 1056, 1074
Hsp92II CATG 2 cut(s) 113, 748
KasI GGCGCC 1 cut(s) 686
Kpn2I TCCGGA 1 cut(s) 855
KroI GCCGGC 4 cut(s) 149, 525, 683, 703
KroNI GCCGGC 4 cut(s) 151, 527, 685, 705
KspAI GTTAAC 1 cut(s) 475
Kzo9I GATC 6 cut(s) 584, 621, 795, 1227, 1305, 1500
LguI GCTCTTC 1 cut(s) 503
LmnI GCTCC 3 cut(s) 858, 1030, 1234
Lsp1109I GCAGC 8 cut(s) 115, 562, 687, 831, 848, 876, 1135, 1448
LweI GCATC 2 cut(s) 180, 1401
MaeII ACGT 8 cut(s) 381, 414, 489, 509, 642, 764, 1074, 1507
MaeIII GTNAC 8 cut(s) 382, 410, 694, 809, 975, 1070, 1108, 1293
MalI GATC 6 cut(s) 586, 623, 797, 1229, 1307, 1502
MboI GATC 6 cut(s) 584, 621, 795, 1227, 1305, 1500
MflI RGATCY 2 cut(s) 1305, 1500
MhlI GDGCHC 3 cut(s) 117, 648, 1239
MlsI TGGCCA 1 cut(s) 1037
MluCI AATT 5 cut(s) 83, 137, 459, 467, 1371
MluNI TGGCCA 1 cut(s) 1037
Mly113I GGCGCC 1 cut(s) 687
MlyI GAGTC 8 cut(s) 293, 314, 591, 605, 614, 914, 1190, 1466
MmeI TCCRAC 7 cut(s) 24, 321, 510, 588, 1205, 1224, 1528
Mox20I TGGCCA 1 cut(s) 1037
MreI CGCCGGCG 2 cut(s) 149, 683
MroI TCCGGA 1 cut(s) 855
MroNI GCCGGC 4 cut(s) 149, 525, 683, 703
MroXI GAANNNNTTC 1 cut(s) 1460
MscI TGGCCA 1 cut(s) 1037
MseI TTAA 4 cut(s) 474, 653, 1242, 1359
MslI CAYNNNNRTG 1 cut(s) 1162
Msp20I TGGCCA 1 cut(s) 1037
MspA1I CMGCKG 2 cut(s) 253, 347
MspR9I CCNGG 5 cut(s) 514, 1002, 1003, 1028, 1133
MvaI CCWGG 1 cut(s) 1028
MvnI CGCG 9 cut(s) 63, 248, 260, 659, 869, 895, 917, 938, 992
MwoI GCNNNNNNNGC 7 cut(s) 155, 245, 269, 353, 689, 875, 1145
NaeI GCCGGC 4 cut(s) 151, 527, 685, 705
NarI GGCGCC 1 cut(s) 687
NciI CCSGG 4 cut(s) 514, 1002, 1003, 1133
NdeII GATC 6 cut(s) 584, 621, 795, 1227, 1305, 1500
NgoMIV GCCGGC 4 cut(s) 149, 525, 683, 703
NlaIII CATG 2 cut(s) 113, 748
NlaIV GGNNCC 8 cut(s) 233, 517, 688, 780, 879, 1006, 1169, 1449
NmeAIII GCCGAG 1 cut(s) 260
NmuCI GTSAC 6 cut(s) 382, 694, 809, 1070, 1108, 1293
NspI RCATGY 1 cut(s) 748
NspV TTCGAA 2 cut(s) 464, 968
PaeR7I CTCGAG 1 cut(s) 1387
PalAI GGCGCGCC 1 cut(s) 915
PauI GCGCGC 2 cut(s) 915, 990
PciSI GCTCTTC 1 cut(s) 503
PcsI WCGNNNNNNNCGW 8 cut(s) 50, 150, 162, 332, 483, 580, 1017, 1053
PdiI GCCGGC 4 cut(s) 151, 527, 685, 705
PdmI GAANNNNTTC 1 cut(s) 1460
PfeI GAWTC 3 cut(s) 337, 455, 717
Pfl23II CGTACG 1 cut(s) 933
PflMI CCANNNNNTGG 1 cut(s) 1033
PfoI TCCNGGA 1 cut(s) 512
PinAI ACCGGT 1 cut(s) 20
PleI GAGTC 8 cut(s) 293, 313, 591, 605, 613, 913, 1190, 1466
PluTI GGCGCC 1 cut(s) 690
PmaCI CACGTG 2 cut(s) 643, 765
PmlI CACGTG 2 cut(s) 643, 765
PpsI GAGTC 8 cut(s) 293, 313, 591, 605, 613, 913, 1190, 1466
Ppu21I YACGTR 3 cut(s) 382, 643, 765
PshBI ATTAAT 1 cut(s) 653
Psp124BI GAGCTC 2 cut(s) 117, 1239
Psp1406I AACGTT 1 cut(s) 414
Psp6I CCWGG 1 cut(s) 1026
PspCI CACGTG 2 cut(s) 643, 765
PspGI CCWGG 1 cut(s) 1026
PspLI CGTACG 1 cut(s) 933
PspN4I GGNNCC 8 cut(s) 233, 517, 688, 780, 879, 1006, 1169, 1449
PspPI GGNCC 5 cut(s) 516, 738, 1005, 1024, 1100
PspXI VCTCGAGB 1 cut(s) 1387
PsrI GAACNNNNNNTAC 2 cut(s) 248, 280
PstI CTGCAG 1 cut(s) 866
PstNI CAGNNNCTG 1 cut(s) 1033
PsuI RGATCY 2 cut(s) 1305, 1500
PteI GCGCGC 2 cut(s) 915, 990
RsaI GTAC 6 cut(s) 125, 266, 573, 770, 935, 1208
RsaNI GTAC 6 cut(s) 124, 265, 572, 769, 934, 1207
RseI CAYNNNNRTG 1 cut(s) 1162
SacI GAGCTC 2 cut(s) 117, 1239
SapI GCTCTTC 1 cut(s) 503
SaqAI TTAA 4 cut(s) 474, 653, 1242, 1359
Sau3AI GATC 6 cut(s) 584, 621, 795, 1227, 1305, 1500
Sau96I GGNCC 5 cut(s) 516, 738, 1005, 1024, 1100
SchI GAGTC 8 cut(s) 293, 314, 591, 605, 614, 914, 1190, 1466
ScrFI CCNGG 5 cut(s) 514, 1002, 1003, 1028, 1133
SduI GDGCHC 3 cut(s) 117, 648, 1239
SfaNI GCATC 2 cut(s) 180, 1401
SfcI CTRYAG 2 cut(s) 592, 862
SfoI GGCGCC 1 cut(s) 688
Sfr274I CTCGAG 1 cut(s) 1387
SfuI TTCGAA 2 cut(s) 464, 968
SgrAI CRCCGGYG 2 cut(s) 149, 683
SgsI GGCGCGCC 1 cut(s) 915
SinI GGWCC 3 cut(s) 516, 1005, 1100
SlaI CTCGAG 1 cut(s) 1387
SmaI CCCGGG 1 cut(s) 1003
SmiMI CAYNNNNRTG 1 cut(s) 1162
SmlI CTYRAG 1 cut(s) 1387
SmoI CTYRAG 1 cut(s) 1387
Sse9I AATT 5 cut(s) 83, 137, 459, 467, 1371
SspDI GGCGCC 1 cut(s) 686
SstI GAGCTC 2 cut(s) 117, 1239
StyD4I CCNGG 5 cut(s) 512, 1000, 1001, 1026, 1131
TaaI ACNGT 6 cut(s) 231, 436, 619, 809, 1247, 1364
TaiI ACGT 8 cut(s) 384, 417, 492, 512, 645, 767, 1077, 1510
TaqII GACCGA 1 cut(s) 1211
TasI AATT 5 cut(s) 83, 137, 459, 467, 1371
TauI GCSGC 9 cut(s) 63, 149, 248, 253, 263, 359, 392, 692, 710
TfiI GAWTC 3 cut(s) 337, 455, 717
Tru1I TTAA 4 cut(s) 474, 653, 1242, 1359
Tru9I TTAA 4 cut(s) 474, 653, 1242, 1359
TscAI CASTG 5 cut(s) 439, 1084, 1290, 1474, 1552
TseFI GTSAC 6 cut(s) 382, 694, 809, 1070, 1108, 1293
TseI GCWGC 8 cut(s) 128, 550, 700, 844, 861, 864, 1148, 1436
Tsp45I GTSAC 6 cut(s) 382, 694, 809, 1070, 1108, 1293
TspDTI ATGAA 2 cut(s) 663, 1453
TspGWI ACGGA 6 cut(s) 61, 589, 617, 663, 959, 997
TspMI CCCGGG 1 cut(s) 1001
TspRI CASTG 5 cut(s) 439, 1084, 1290, 1474, 1552
Van91I CCANNNNNTGG 1 cut(s) 1033
VneI GTGCAC 1 cut(s) 644
VpaK11BI GGWCC 3 cut(s) 516, 1005, 1100
VspI ATTAAT 1 cut(s) 653
XapI RAATTY 2 cut(s) 83, 467
XceI RCATGY 1 cut(s) 748
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XhoI CTCGAG 1 cut(s) 1387
XmaI CCCGGG 1 cut(s) 1001
XmnI GAANNNNTTC 1 cut(s) 1460
ZraI GACGTC 1 cut(s) 1075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.