Rorug07G0046000

DNA-directed RNA polymerase III subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0007770
Physical Location & Seq
Reverse (-)
3357758 .. 3360746
2989 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0046000.1

Sequence Viewer

Length: 1203 bp
ATGGCGGATCAGCTAATGGATCTCCCGCATACCACAGGAGCAAGTTTTGCAAAGACTTGCTTCAATGGGTTCAATGCCCTATCAAGAATTGGATTGCTTACCACCCCTTATGCACTTTCAAAATCTGGATGGTTAAGTCTTGGTCTTCTTCTAGTTATGGCAATAACAACTTGCTACACTGGAATCTTGCTCACCAAATGCTTGAACACGAATAGATCAATGAAGACATATTCTGACGTGGCTTTCCGGGCATTTGGGCGAAAGGGCAGAATATTCGTTTCCATTTTCTTATACCTAGAGGTTTACCTAGTCCCAACAGGATTGCTGATAATGGAGGCTGATAATTTAGTTAAGTTATTCCCACACCTTGGAGTCAAGGGAGTCAATTTCGGTGGACTTCACGTGGCAGGAAAACCATTTTTCACAATGCTTGTGGGTCTGATAATATTACCTTCGATGTGGTTAAAGGACCTGAGATTGCTTTCCTACATCTCTTTATGTGGGATACTTTCAACCATTGTTATCATCAGCGCCATTGTTTGGGTTGGTATTGATGAAGTAGGGTTCTCCGCAGCAGGAGAGATATTCGATTGGAAAGGACTTCCTACTGCTCTTAGTTTGTATGCATTTTGTTATGGAGCTCATCCAGTCTTTCCAGTCCTATATTCTTCAATGAAACAAAAAAATCACTTCTTCAAGGTTTTGCTCATCACTTTTTTCCTATGCACTGTAAACTACATGCTGGTGGCGATTCTTGGCTATTTGATGTTTGGCAAGAATGTCCAATCTCAAGTGACACTGAACATGTCTACAAAACTCAGCTCAAAAATAGTCATATACACCAGTTTAGCTGCTCCGATGGTTAAGTATGCACTGATAATCTCACCAATAGCAGATGCCATTGAGATGAATTTACCGAGTAAATGGAAAAGCAAGCTGGTTTGGATACTCGTAAGGACATTGTTATTGGTTAGCACAGTTGTAGTGGCTTTAGTTTTTCCCTATTTTGAGTCACTAACGGGACTTGTTGGAGCAATTCTTTGCGTAACGATTTCCATCTTGCTTCCATGTATTTGCTACTTGAAGATATTTAGTCTATACCAGAAGTTTGATGTGGAAGTTTTGATTATTGGAAGCATTATGGTTATGGCTGTAGCTGTTGGTGTGATGGGCACATATGCATCTGTTAAAACGAGAGAGTGA

Protein Analysis

400

Amino Acids

44.02

Weight (kDa)

9.32

Isoelectric Point (pI)

24.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 14 - 392 1.1e-59 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 368, 540
AccI GTMKAC 1 cut(s) 809
AciI CCGC 3 cut(s) 5, 26, 570
AclWI GGATC 2 cut(s) 15, 27
AcsI RAATTY 1 cut(s) 910
AcvI CACGTG 1 cut(s) 403
AfiI CCNNNNNNNGG 2 cut(s) 368, 540
AflIII ACRYGT 1 cut(s) 804
AgsI TTSAA 8 cut(s) 64, 73, 120, 205, 513, 672, 697, 1084
AjiI CACGTC 1 cut(s) 238
AluBI AGCT 6 cut(s) 13, 641, 822, 851, 937, 1157
AluI AGCT 6 cut(s) 13, 641, 822, 851, 937, 1157
Alw21I GWGCWC 1 cut(s) 643
AlwI GGATC 2 cut(s) 15, 27
ApeKI GCWGC 2 cut(s) 572, 851
ApoI RAATTY 1 cut(s) 910
AspLEI GCGC 1 cut(s) 533
AspS9I GGNCC 1 cut(s) 469
AsuC2I CCSGG 1 cut(s) 248
AsuHPI GGTGA 2 cut(s) 184, 876
AvaII GGWCC 1 cut(s) 469
BaeGI GKGCMC 1 cut(s) 1175
BanII GRGCYC 1 cut(s) 643
BbrPI CACGTG 1 cut(s) 403
BbsI GAAGAC 2 cut(s) 137, 230
Bbv12I GWGCWC 1 cut(s) 643
BbvI GCAGC 2 cut(s) 584, 838
BccI CCATC 4 cut(s) 123, 853, 1064, 1162
BcgI CGANNNNNNTGC 2 cut(s) 256, 290
BciVI GTATCC 2 cut(s) 498, 939
BcnI CCSGG 1 cut(s) 248
BfaI CTAG 3 cut(s) 152, 296, 308
BfmI CTRYAG 1 cut(s) 1152
BfoI RGCGCY 1 cut(s) 534
BfuI GTATCC 2 cut(s) 498, 939
BisI GCNGC 2 cut(s) 573, 852
BlsI GCNGC 2 cut(s) 574, 853
Bme1390I CCNGG 1 cut(s) 248
Bme18I GGWCC 1 cut(s) 469
BmgBI CACGTC 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 469
BmrFI CCNGG 1 cut(s) 248
BmsI GCATC 2 cut(s) 886, 1190
BpiI GAAGAC 2 cut(s) 137, 230
BpuEI CTTGAG 1 cut(s) 774
BpuMI CCSGG 1 cut(s) 248
BsaAI YACGTR 1 cut(s) 403
BsaBI GATNNNNATC 1 cut(s) 1055
BsaJI CCNNGG 1 cut(s) 367
Bsc4I CCNNNNNNNGG 2 cut(s) 368, 540
Bse1I ACTGG 4 cut(s) 184, 647, 656, 843
Bse8I GATNNNNATC 1 cut(s) 1055
BseDI CCNNGG 1 cut(s) 367
BseGI GGATG 2 cut(s) 134, 643
BseJI GATNNNNATC 1 cut(s) 1055
BseLI CCNNNNNNNGG 2 cut(s) 368, 540
BseMII CTCAG 2 cut(s) 464, 832
BseNI ACTGG 4 cut(s) 184, 647, 656, 843
BseSI GKGCMC 1 cut(s) 1175
BseXI GCAGC 2 cut(s) 584, 838
BsiHKAI GWGCWC 1 cut(s) 643
BsiSI CCGG 1 cut(s) 247
BslFI GGGAC 2 cut(s) 296, 1035
BslI CCNNNNNNNGG 2 cut(s) 368, 540
BsmFI GGGAC 2 cut(s) 296, 1035
Bsp1286I GDGCHC 2 cut(s) 643, 1175
Bsp143I GATC 3 cut(s) 7, 19, 215
BspACI CCGC 3 cut(s) 5, 26, 570
BspCNI CTCAG 2 cut(s) 465, 831
BspPI GGATC 2 cut(s) 15, 27
BsrI ACTGG 4 cut(s) 184, 647, 656, 843
BssECI CCNNGG 1 cut(s) 367
BssMI GATC 3 cut(s) 7, 19, 215
BssT1I CCWWGG 1 cut(s) 367
Bst4CI ACNGT 2 cut(s) 730, 979
BstAPI GCANNNNNTGC 1 cut(s) 47
BstBAI YACGTR 1 cut(s) 403
BstC8I GCNNGC 1 cut(s) 935
BstDEI CTNAG 3 cut(s) 473, 614, 818
BstF5I GGATG 2 cut(s) 134, 643
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 1 cut(s) 533
BstKTI GATC 3 cut(s) 10, 22, 218
BstMBI GATC 3 cut(s) 7, 19, 215
BstMWI GCNNNNNNNGC 2 cut(s) 47, 248
BstNSI RCATGY 2 cut(s) 742, 808
BstSCI CCNGG 1 cut(s) 246
BstSFI CTRYAG 1 cut(s) 1152
BstSLI GKGCMC 1 cut(s) 1175
BstV1I GCAGC 2 cut(s) 584, 838
BstV2I GAAGAC 2 cut(s) 137, 230
BstX2I RGATCY 1 cut(s) 19
BstYI RGATCY 1 cut(s) 19
BsuI GTATCC 2 cut(s) 498, 939
BtrI CACGTC 1 cut(s) 238
BtsCI GGATG 2 cut(s) 134, 643
BtsIMutI CAGTG 4 cut(s) 177, 726, 797, 872
Cac8I GCNNGC 1 cut(s) 935
CfoI GCGC 1 cut(s) 533
Cfr13I GGNCC 1 cut(s) 469
CspCI CAANNNNNGTGG 4 cut(s) 373, 408, 414, 449
CviAII CATG 3 cut(s) 739, 805, 1068
DdeI CTNAG 3 cut(s) 473, 614, 818
DpnI GATC 3 cut(s) 9, 21, 217
DpnII GATC 3 cut(s) 7, 19, 215
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 641
Eco130I CCWWGG 1 cut(s) 367
Eco24I GRGCYC 1 cut(s) 643
Eco47I GGWCC 1 cut(s) 469
Eco53kI GAGCTC 1 cut(s) 641
Eco72I CACGTG 1 cut(s) 403
EcoICRI GAGCTC 1 cut(s) 641
EcoO109I RGGNCCY 1 cut(s) 469
EcoT14I CCWWGG 1 cut(s) 367
EcoT22I ATGCAT 2 cut(s) 628, 1183
EcoT38I GRGCYC 1 cut(s) 643
ErhI CCWWGG 1 cut(s) 367
FaeI CATG 3 cut(s) 742, 808, 1071
FalI AAGNNNNNCTT 2 cut(s) 44, 76
FaqI GGGAC 2 cut(s) 296, 1035
FatI CATG 3 cut(s) 738, 804, 1067
FauI CCCGC 1 cut(s) 33
FauNDI CATATG 1 cut(s) 1177
FblI GTMKAC 1 cut(s) 809
Fnu4HI GCNGC 2 cut(s) 573, 852
FokI GGATG 2 cut(s) 141, 630
FriOI GRGCYC 1 cut(s) 643
Fsp4HI GCNGC 2 cut(s) 573, 852
FspBI CTAG 3 cut(s) 152, 296, 308
GlaI GCGC 1 cut(s) 532
GluI GCNGC 2 cut(s) 573, 852
HaeII RGCGCY 1 cut(s) 534
HapII CCGG 1 cut(s) 247
HhaI GCGC 1 cut(s) 533
Hin1II CATG 3 cut(s) 742, 808, 1071
Hin6I GCGC 1 cut(s) 531
HinP1I GCGC 1 cut(s) 531
HinfI GANTC 5 cut(s) 183, 372, 381, 751, 1010
HpaII CCGG 1 cut(s) 247
HphI GGTGA 2 cut(s) 184, 876
Hpy166II GTNNAC 4 cut(s) 304, 395, 733, 810
Hpy188I TCNGA 3 cut(s) 235, 441, 858
Hpy188III TCNNGA 2 cut(s) 84, 126
Hpy8I GTNNAC 4 cut(s) 304, 395, 733, 810
HpyAV CCTTC 1 cut(s) 462
HpyCH4III ACNGT 2 cut(s) 730, 979
HpyCH4IV ACGT 2 cut(s) 237, 402
HpyCH4V TGCA 6 cut(s) 50, 113, 626, 726, 872, 1181
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 248
HpyF3I CTNAG 3 cut(s) 473, 614, 818
HpySE526I ACGT 2 cut(s) 237, 402
Hsp92II CATG 3 cut(s) 742, 808, 1071
HspAI GCGC 1 cut(s) 531
Kzo9I GATC 3 cut(s) 7, 19, 215
LmnI GCTCC 4 cut(s) 38, 638, 859, 1031
Lsp1109I GCAGC 2 cut(s) 584, 838
LweI GCATC 2 cut(s) 886, 1190
MaeI CTAG 3 cut(s) 152, 296, 308
MaeII ACGT 2 cut(s) 237, 402
MaeIII GTNAC 3 cut(s) 793, 1011, 1045
MalI GATC 3 cut(s) 9, 21, 217
MboI GATC 3 cut(s) 7, 19, 215
MboII GAAGA 6 cut(s) 137, 140, 235, 660, 685, 1096
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 2 cut(s) 643, 1175
MluCI AATT 5 cut(s) 87, 343, 385, 910, 1035
MlyI GAGTC 3 cut(s) 381, 390, 1019
MmeI TCCRAC 1 cut(s) 1009
MnlI CCTC 2 cut(s) 292, 328
Mph1103I ATGCAT 2 cut(s) 628, 1183
MseI TTAA 5 cut(s) 134, 351, 464, 864, 1188
MslI CAYNNNNRTG 2 cut(s) 743, 905
MspI CCGG 1 cut(s) 247
MspR9I CCNGG 1 cut(s) 248
MwoI GCNNNNNNNGC 2 cut(s) 47, 248
NciI CCSGG 1 cut(s) 248
NdeI CATATG 1 cut(s) 1177
NdeII GATC 3 cut(s) 7, 19, 215
NlaIII CATG 3 cut(s) 742, 808, 1071
NmuCI GTSAC 2 cut(s) 793, 1011
NsiI ATGCAT 2 cut(s) 628, 1183
NspI RCATGY 2 cut(s) 742, 808
PciI ACATGT 1 cut(s) 804
PfeI GAWTC 2 cut(s) 183, 751
PflMI CCANNNNNTGG 2 cut(s) 368, 540
PkrI GCNGC 2 cut(s) 574, 853
PleI GAGTC 3 cut(s) 380, 389, 1018
PmaCI CACGTG 1 cut(s) 403
PmlI CACGTG 1 cut(s) 403
PpsI GAGTC 3 cut(s) 380, 389, 1018
Ppu21I YACGTR 1 cut(s) 403
PpuMI RGGWCCY 1 cut(s) 469
PscI ACATGT 1 cut(s) 804
Psp124BI GAGCTC 1 cut(s) 643
Psp5II RGGWCCY 1 cut(s) 469
PspCI CACGTG 1 cut(s) 403
PspPI GGNCC 1 cut(s) 469
PspPPI RGGWCCY 1 cut(s) 469
PsuI RGATCY 1 cut(s) 19
RseI CAYNNNNRTG 2 cut(s) 743, 905
SacI GAGCTC 1 cut(s) 643
SaqAI TTAA 5 cut(s) 134, 351, 464, 864, 1188
SatI GCNGC 2 cut(s) 573, 852
Sau3AI GATC 3 cut(s) 7, 19, 215
Sau96I GGNCC 1 cut(s) 469
SchI GAGTC 3 cut(s) 381, 390, 1019
ScrFI CCNGG 1 cut(s) 248
SduI GDGCHC 2 cut(s) 643, 1175
SfaNI GCATC 2 cut(s) 886, 1190
SfcI CTRYAG 1 cut(s) 1152
SinI GGWCC 1 cut(s) 469
SmiMI CAYNNNNRTG 2 cut(s) 743, 905
SmlI CTYRAG 1 cut(s) 789
SmoI CTYRAG 1 cut(s) 789
Sse9I AATT 5 cut(s) 87, 343, 385, 910, 1035
SsiI CCGC 3 cut(s) 5, 26, 570
SspI AATATT 2 cut(s) 273, 447
SspMI CTAG 3 cut(s) 152, 296, 308
SstI GAGCTC 1 cut(s) 643
StyD4I CCNGG 1 cut(s) 246
StyI CCWWGG 1 cut(s) 367
TaaI ACNGT 2 cut(s) 730, 979
TaiI ACGT 2 cut(s) 240, 405
TaqI TCGA 2 cut(s) 455, 588
TasI AATT 5 cut(s) 87, 343, 385, 910, 1035
TfiI GAWTC 2 cut(s) 183, 751
Tru1I TTAA 5 cut(s) 134, 351, 464, 864, 1188
Tru9I TTAA 5 cut(s) 134, 351, 464, 864, 1188
TscAI CASTG 4 cut(s) 184, 733, 804, 879
TseFI GTSAC 2 cut(s) 793, 1011
TseI GCWGC 2 cut(s) 572, 851
Tsp45I GTSAC 2 cut(s) 793, 1011
TspDTI ATGAA 4 cut(s) 236, 570, 689, 923
TspRI CASTG 4 cut(s) 184, 733, 804, 879
Van91I CCANNNNNTGG 2 cut(s) 368, 540
VpaK11BI GGWCC 1 cut(s) 469
XapI RAATTY 1 cut(s) 910
XceI RCATGY 2 cut(s) 742, 808
XmiI GTMKAC 1 cut(s) 809
XspI CTAG 3 cut(s) 152, 296, 308
Zsp2I ATGCAT 2 cut(s) 628, 1183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.