FvH4_5g02070

Serine racemase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007770
Physical Location & Seq
Reverse (-)
1294625 .. 1298004
3380 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g02070.t1

Sequence Viewer

Length: 1005 bp
ATGGAAGCAGAGAGTCAAGTGAGGGAAGTGAAATATGCTGCTGATATTTCGTCTATTAAAGAAGCTCAAGCACGCATCAAGCCATTCATACATGAAACTCCAGTCCTGTCCTCTGAAACTTTGAATCTTATTGCAGGAAGACAGCTGTTTTTCAAATGTGAATGTTTCCAAAAGGGTGGCGCTTTCAAATTTAGAGGCGCTTGCAATGCTGTGTTTTCACTTGATGATGATCAGGCTGCTAAAGGGGTTGTGACACACAGCAGTGGTAACCATGCTGCAGCATTGTCTTTGGCTGCAAAACTACGGGGTATTCCTGCATATATAGTTATACCGAAAAATGCCCCAAAATGCAAAGTTGAGAATGTCATGCGTTACGGTGGTCAGGTAATCTGGAGTGAACCAACAATGCAGTCAAGGGAAAGTACTGCAGCCAAGGTACTGCAAGAAACTGGCGCAGTTCTTCTACATCCCTACAATGATGAGCGGATAATAAGTGGCCAGGGTACCATTTCGTTGGAGCTACTGGAGCAACAAGTTCCACAGTTAGACACTATAATCGTTCCCATAAGTGGAGGTGGCTTGATATCAGGTGTGGCTTTGGCTGCCAAGTATATCAATCCTGCCATTCGAGTTTTAGCTGCTGAACCAAAAGGAGCTAACGATGCAGCTCAATCTAAAGAAGCTGGAAGGATAATAACATTGCCTGAGACCAACACTGTAGCAGATGGGCTTCGAGCTTTTCTGGGAGATCTAACCTGGCCAGTAGTAAGAGATCTTGTCGACGGCGTAATAACTGTAGAGGACAAGGAGATAATAAACGCAATGAAACTCTGTTATGAGATTCTGAAGGTTGCAGTAGAACCTAGCGGAGCAATAGGCCTCGCAGCTGTTTTATCTGATAGTTTCAAGAACAATACTGCTTGGAAGGATTGCACCAATATAGGAATTGTTCTTTCTGGAGGTAATGTGGATCTAGGCATCCTATGGGATTCATACACTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000287 GO:0002237 GO:0003674 GO:0003824 GO:0003941 GO:0005488 GO:0005509 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006090 GO:0006520 GO:0006563 GO:0006564 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008652 GO:0008721 GO:0009058 GO:0009069 GO:0009070 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0016043 GO:0016053 GO:0016594 GO:0016597 GO:0016829 GO:0016840 GO:0016841 GO:0016853 GO:0016854 GO:0016855 GO:0017076 GO:0017144 GO:0018114 GO:0019752 GO:0019842 GO:0019904 GO:0022607 GO:0030165 GO:0030170 GO:0030378 GO:0030554 GO:0031406 GO:0032496 GO:0032553 GO:0032555 GO:0032559 GO:0032787 GO:0033993 GO:0035639 GO:0036094 GO:0036361 GO:0036477 GO:0042165 GO:0042221 GO:0042802 GO:0042803 GO:0042866 GO:0043025 GO:0043167 GO:0043168 GO:0043169 GO:0043177 GO:0043207 GO:0043436 GO:0043621 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044297 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046416 GO:0046437 GO:0046872 GO:0046983 GO:0047661 GO:0048037 GO:0050662 GO:0050896 GO:0051259 GO:0051260 GO:0051262 GO:0051289 GO:0051704 GO:0051707 GO:0065003 GO:0070178 GO:0070179 GO:0070279 GO:0071704 GO:0071840 GO:0072330 GO:0097159 GO:0097367 GO:0097458 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00260 ko01100 map00260 map01100
Pfam Domains
Protein Families
PALP

Protein Analysis

335

Amino Acids

35.75

Weight (kDa)

5.71

Isoelectric Point (pI)

27.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 27 - 320 1.5e-75 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 503
AccB1I GGYRCC 1 cut(s) 503
AccBSI CCGCTC 1 cut(s) 484
AccI GTMKAC 1 cut(s) 780
AciI CCGC 2 cut(s) 484, 867
AclWI GGATC 1 cut(s) 978
AcoI YGGCCR 2 cut(s) 496, 758
AcsI RAATTY 1 cut(s) 188
AcuI CTGAAG 1 cut(s) 866
AfaI GTAC 3 cut(s) 424, 438, 505
AfiI CCNNNNNNNGG 1 cut(s) 569
AgsI TTSAA 4 cut(s) 124, 154, 187, 907
AjnI CCWGG 2 cut(s) 498, 755
AleI CACNNNNGTG 1 cut(s) 261
AluBI AGCT 9 cut(s) 65, 145, 520, 638, 656, 668, 683, 737, 887
AluI AGCT 9 cut(s) 65, 145, 520, 638, 656, 668, 683, 737, 887
Alw26I GTCTC 1 cut(s) 701
AlwI GGATC 1 cut(s) 978
AoxI GGCC 3 cut(s) 496, 758, 877
ApoI RAATTY 1 cut(s) 188
Asp718I GGTACC 1 cut(s) 503
AspLEI GCGC 3 cut(s) 182, 200, 455
BalI TGGCCA 2 cut(s) 498, 760
BanI GGYRCC 1 cut(s) 503
BbsI GAAGAC 1 cut(s) 145
BccI CCATC 1 cut(s) 719
BceAI ACGGC 1 cut(s) 799
BciT130I CCWGG 2 cut(s) 500, 757
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 1 cut(s) 701
BfaI CTAG 2 cut(s) 864, 974
BfmI CTRYAG 4 cut(s) 276, 426, 717, 795
BfoI RGCGCY 2 cut(s) 183, 201
BglII AGATCT 2 cut(s) 748, 772
BmcAI AGTACT 1 cut(s) 424
Bme1390I CCNGG 2 cut(s) 500, 757
BmiI GGNNCC 1 cut(s) 505
BmrFI CCNGG 2 cut(s) 500, 757
BmsI GCATC 3 cut(s) 84, 652, 987
BpiI GAAGAC 1 cut(s) 145
BpmI CTGGAG 4 cut(s) 84, 412, 545, 978
BpuEI CTTGAG 1 cut(s) 51
BsaBI GATNNNNATC 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 701
BsaJI CCNNGG 2 cut(s) 432, 499
BsaXI ACNNNNNCTCC 2 cut(s) 951, 981
Bsc4I CCNNNNNNNGG 1 cut(s) 569
Bse1I ACTGG 4 cut(s) 101, 454, 528, 761
Bse3DI GCAATG 3 cut(s) 211, 698, 828
Bse8I GATNNNNATC 1 cut(s) 228
BseBI CCWGG 2 cut(s) 500, 757
BseDI CCNNGG 2 cut(s) 432, 499
BseGI GGATG 2 cut(s) 466, 978
BseJI GATNNNNATC 1 cut(s) 228
BseLI CCNNNNNNNGG 1 cut(s) 569
BseMI GCAATG 3 cut(s) 211, 698, 828
BseMII CTCAG 1 cut(s) 696
BseNI ACTGG 4 cut(s) 101, 454, 528, 761
BshFI GGCC 3 cut(s) 498, 760, 879
BshNI GGYRCC 1 cut(s) 503
BslI CCNNNNNNNGG 1 cut(s) 569
BsmAI GTCTC 1 cut(s) 701
BsnI GGCC 3 cut(s) 498, 760, 879
Bso31I GGTCTC 1 cut(s) 701
Bsp143I GATC 4 cut(s) 229, 748, 772, 970
BspACI CCGC 2 cut(s) 484, 867
BspANI GGCC 3 cut(s) 498, 760, 879
BspCNI CTCAG 1 cut(s) 697
BspLI GGNNCC 1 cut(s) 505
BspMAI CTGCAG 2 cut(s) 280, 430
BspPI GGATC 1 cut(s) 978
BspT107I GGYRCC 1 cut(s) 503
BspTNI GGTCTC 1 cut(s) 701
BsrBI CCGCTC 1 cut(s) 484
BsrDI GCAATG 3 cut(s) 211, 698, 828
BsrI ACTGG 4 cut(s) 101, 454, 528, 761
BssECI CCNNGG 2 cut(s) 432, 499
BssMI GATC 4 cut(s) 229, 748, 772, 970
BssT1I CCWWGG 1 cut(s) 432
Bst2UI CCWGG 2 cut(s) 500, 757
Bst4CI ACNGT 4 cut(s) 377, 543, 718, 796
BstC8I GCNNGC 2 cut(s) 73, 202
BstDEI CTNAG 1 cut(s) 705
BstEII GGTNACC 1 cut(s) 266
BstF5I GGATG 2 cut(s) 466, 978
BstH2I RGCGCY 2 cut(s) 183, 201
BstHHI GCGC 3 cut(s) 182, 200, 455
BstKTI GATC 4 cut(s) 232, 751, 775, 973
BstMAI GTCTC 1 cut(s) 701
BstMBI GATC 4 cut(s) 229, 748, 772, 970
BstMWI GCNNNNNNNGC 4 cut(s) 206, 526, 602, 662
BstNI CCWGG 2 cut(s) 500, 757
BstPI GGTNACC 1 cut(s) 266
BstSCI CCNGG 2 cut(s) 498, 755
BstSFI CTRYAG 4 cut(s) 276, 426, 717, 795
BstV2I GAAGAC 1 cut(s) 145
BstX2I RGATCY 3 cut(s) 748, 772, 970
BstXI CCANNNNNNTGG 2 cut(s) 176, 514
BstYI RGATCY 3 cut(s) 748, 772, 970
BsuRI GGCC 3 cut(s) 498, 760, 879
BtsCI GGATG 2 cut(s) 466, 978
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 2 cut(s) 268, 714
Cac8I GCNNGC 2 cut(s) 73, 202
CfoI GCGC 3 cut(s) 182, 200, 455
Csp6I GTAC 3 cut(s) 423, 437, 504
CviAII CATG 3 cut(s) 92, 272, 367
CviQI GTAC 3 cut(s) 423, 437, 504
DdeI CTNAG 1 cut(s) 705
DpnI GATC 4 cut(s) 231, 750, 774, 972
DpnII GATC 4 cut(s) 229, 748, 772, 970
EaeI YGGCCR 2 cut(s) 496, 758
Eco130I CCWWGG 1 cut(s) 432
Eco147I AGGCCT 1 cut(s) 879
Eco31I GGTCTC 1 cut(s) 701
Eco32I GATATC 1 cut(s) 585
Eco57I CTGAAG 1 cut(s) 866
Eco91I GGTNACC 1 cut(s) 266
EcoO65I GGTNACC 1 cut(s) 266
EcoRII CCWGG 2 cut(s) 498, 755
EcoRV GATATC 1 cut(s) 585
EcoT14I CCWWGG 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 432
FaeI CATG 3 cut(s) 95, 275, 370
FatI CATG 3 cut(s) 91, 271, 366
FbaI TGATCA 1 cut(s) 229
FblI GTMKAC 1 cut(s) 780
FokI GGATG 2 cut(s) 453, 965
FspBI CTAG 2 cut(s) 864, 974
GlaI GCGC 3 cut(s) 181, 199, 454
GsuI CTGGAG 4 cut(s) 84, 412, 545, 978
HaeII RGCGCY 2 cut(s) 183, 201
HaeIII GGCC 3 cut(s) 498, 760, 879
HhaI GCGC 3 cut(s) 182, 200, 455
Hin1II CATG 3 cut(s) 95, 275, 370
Hin6I GCGC 3 cut(s) 180, 198, 453
HinP1I GCGC 3 cut(s) 180, 198, 453
HincII GTYRAC 1 cut(s) 781
HindII GTYRAC 1 cut(s) 781
HinfI GANTC 4 cut(s) 13, 124, 841, 989
Hpy166II GTNNAC 2 cut(s) 398, 781
Hpy188I TCNGA 3 cut(s) 115, 846, 898
Hpy188III TCNNGA 3 cut(s) 391, 907, 957
Hpy8I GTNNAC 2 cut(s) 398, 781
Hpy99I CGWCG 1 cut(s) 785
HpyAV CCTTC 3 cut(s) 681, 841, 919
HpyCH4III ACNGT 4 cut(s) 377, 543, 718, 796
HpyF10VI GCNNNNNNNGC 4 cut(s) 206, 526, 602, 662
HpyF3I CTNAG 1 cut(s) 705
Hsp92II CATG 3 cut(s) 95, 275, 370
HspAI GCGC 3 cut(s) 180, 198, 453
KpnI GGTACC 1 cut(s) 507
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 4 cut(s) 229, 748, 772, 970
LmnI GCTCC 4 cut(s) 517, 526, 653, 869
LweI GCATC 3 cut(s) 84, 652, 987
MaeI CTAG 2 cut(s) 864, 974
MaeIII GTNAC 3 cut(s) 250, 266, 371
MalI GATC 4 cut(s) 231, 750, 774, 972
MbiI CCGCTC 1 cut(s) 484
MboI GATC 4 cut(s) 229, 748, 772, 970
MboII GAAGA 2 cut(s) 150, 452
MflI RGATCY 3 cut(s) 748, 772, 970
MlsI TGGCCA 2 cut(s) 498, 760
MluCI AATT 2 cut(s) 188, 945
MluNI TGGCCA 2 cut(s) 498, 760
MlyI GAGTC 1 cut(s) 22
MmeI TCCRAC 1 cut(s) 495
MnlI CCTC 7 cut(s) 15, 121, 188, 566, 793, 890, 953
Mox20I TGGCCA 2 cut(s) 498, 760
MscI TGGCCA 2 cut(s) 498, 760
MseI TTAA 1 cut(s) 57
MslI CAYNNNNRTG 1 cut(s) 261
Msp20I TGGCCA 2 cut(s) 498, 760
MspA1I CMGCKG 2 cut(s) 145, 887
MspR9I CCNGG 2 cut(s) 500, 757
MvaI CCWGG 2 cut(s) 500, 757
MwoI GCNNNNNNNGC 4 cut(s) 206, 526, 602, 662
NdeII GATC 4 cut(s) 229, 748, 772, 970
NlaIII CATG 3 cut(s) 95, 275, 370
NlaIV GGNNCC 1 cut(s) 505
NmuCI GTSAC 1 cut(s) 250
OliI CACNNNNGTG 1 cut(s) 261
PceI AGGCCT 1 cut(s) 879
PfeI GAWTC 3 cut(s) 124, 841, 989
PleI GAGTC 1 cut(s) 21
PpsI GAGTC 1 cut(s) 21
Psp6I CCWGG 2 cut(s) 498, 755
PspEI GGTNACC 1 cut(s) 266
PspGI CCWGG 2 cut(s) 498, 755
PspN4I GGNNCC 1 cut(s) 505
PstI CTGCAG 2 cut(s) 280, 430
PsuI RGATCY 3 cut(s) 748, 772, 970
PvuII CAGCTG 2 cut(s) 145, 887
RsaI GTAC 3 cut(s) 424, 438, 505
RsaNI GTAC 3 cut(s) 423, 437, 504
RseI CAYNNNNRTG 1 cut(s) 261
SalI GTCGAC 1 cut(s) 779
SaqAI TTAA 1 cut(s) 57
Sau3AI GATC 4 cut(s) 229, 748, 772, 970
ScaI AGTACT 1 cut(s) 424
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 2 cut(s) 500, 757
SfaNI GCATC 3 cut(s) 84, 652, 987
SfcI CTRYAG 4 cut(s) 276, 426, 717, 795
SmiMI CAYNNNNRTG 1 cut(s) 261
SmlI CTYRAG 1 cut(s) 66
SmoI CTYRAG 1 cut(s) 66
Sse9I AATT 2 cut(s) 188, 945
SseBI AGGCCT 1 cut(s) 879
SsiI CCGC 2 cut(s) 484, 867
SspMI CTAG 2 cut(s) 864, 974
StuI AGGCCT 1 cut(s) 879
StyD4I CCNGG 2 cut(s) 498, 755
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 4 cut(s) 377, 543, 718, 796
TaqI TCGA 3 cut(s) 628, 733, 780
TasI AATT 2 cut(s) 188, 945
TatI WGTACW 1 cut(s) 422
TfiI GAWTC 3 cut(s) 124, 841, 989
Tru1I TTAA 1 cut(s) 57
Tru9I TTAA 1 cut(s) 57
TscAI CASTG 2 cut(s) 268, 721
TseFI GTSAC 1 cut(s) 250
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 4 cut(s) 76, 108, 839, 981
TspRI CASTG 2 cut(s) 268, 721
XapI RAATTY 1 cut(s) 188
XmiI GTMKAC 1 cut(s) 780
XspI CTAG 2 cut(s) 864, 974
ZrmI AGTACT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.