Rh7DG172000

4-coumarate--CoA ligase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0008550
Physical Location & Seq
Forward (+)
15632039 .. 15636146
4108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG172000.1

Sequence Viewer

Length: 1287 bp
ATGGAAAACCCGACCCTGACCGGTTTGTTGAAGAAGGTCGCCTCGGAATTCCCGAACCGCCGCGCTATTTCCGTTTCCGGCAAATTCGACTTGACCCATGCCCGATTACAGGAGCTCGTCGAGCACGCCGCCTCTCAATTACTCGCCGCTGGCATCGGTCCTCACGACACCGTCGCTCTCACCTTCCCCAACACCATTGAGTTTGTGGTGATGTTCTTGGCGGTGATAAGGTGCCGAGCCACGGCCGCGCCGCTTAACGCGGCGTACACGGCGGAGGAGTTCGAGTTCTACCTCTCCGACTCCGAGTCGAAGCTTCTGATCACGGCGGAGAAGCCAATTCAGTCGGCTCTGACCGCCGCTTCCAAGCTCAGCATCCCTTACGTGACGGCGTCGCTGCCCGACTCCGCCGCACATATTTCTCTGTCTTCAACCACCACCGCCGGCAAGCCGAGTTCCGACTCGGTTTCGGAACTCGTTAACGACCCGTCCGACGTGGCACTCTTCCTCCACACGTCGGGGACCACGAGCCGGCCCAAGGGGGTTCCCCTGACCCAGCTCAATCTGGCCTCGTCGGTTCGGAACATCCGGTCGGTCTATAAACTCACCGAGTCGGACTCGACGGTGATCGTCCTGCCGTTGTTTCACGTGCACGGGTTAATCGCCGGCTTACTGAGTTCACTCACCGCCGGAGCGGCCGCGACGCTCCCCGCCGCCGGCCGATTCTCCGCTTCGACGTTTTGGTCAGACATGCTAGCATACAACGCCACGTGGTACACTGCGGTGCCCACCATCCACCAGATCATTCTCGACCGTCACGCCAACAAACCCGAACCGGCCTACCCGAAGCTCCGGTTTATCCGGAGCTGCAGCGCGTCGCTGGCACCGTCAATTTTGACGCGTTTGGAGGAGTCGTTCGGCGCGCCGGTGCTGGAGGCTTACGCGATGACGGAGGCGACCCATTTGATGTGTTCGAATCCGTTACCCGAAGACGGCGAGCACAAACCCGGGTCCGTCGGGATACCCGTGGGCCAGGAATTGGCCATCTTGAACGAGAACGGCGTCGTTCAGCCAAATGACGTCAGCGGCGAGGTGTGCATTAGAGGACCTAATGTGACAAAAGGGTATAAGAACAACCCGGAAGCAAACAAAGCGGCTTTCACATTCGGGTGGTTCCACACAGGAGATGTTGGGTTTTTGGACTCCGATGGGTACTTGCACCTTGTGGGTCGGATCAAGGAGCTCATTAACCGTGGAGGTAATTTAATCGCAACACAACATGTTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00630 ko01100 map00630 map01100
Pfam Domains
Protein Families
AMP-binding AMP-binding_C Pkinase

Protein Analysis

428

Amino Acids

45.77

Weight (kDa)

6.16

Isoelectric Point (pI)

37.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 11 - 375 4.7e-83 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 739
AatII GACGTC 1 cut(s) 1080
AccB1I GGYRCC 3 cut(s) 231, 781, 880
AccB7I CCANNNNNTGG 1 cut(s) 1036
AccBSI CCGCTC 1 cut(s) 692
AccII CGCG 8 cut(s) 63, 248, 260, 698, 872, 898, 920, 941
AccIII TCCGGA 1 cut(s) 858
AclWI GGATC 1 cut(s) 1238
AcoI YGGCCR 4 cut(s) 243, 693, 715, 1038
AcsI RAATTY 2 cut(s) 47, 83
AcvI CACGTG 2 cut(s) 646, 768
AcyI GRCGYC 3 cut(s) 389, 1059, 1077
AdeI CACNNNGTG 1 cut(s) 1222
AfaI GTAC 3 cut(s) 266, 773, 1211
AfiI CCNNNNNNNGG 8 cut(s) 109, 241, 514, 528, 535, 713, 989, 1036
AflIII ACRYGT 3 cut(s) 510, 896, 1276
AgeI ACCGGT 1 cut(s) 20
AgsI TTSAA 3 cut(s) 31, 429, 1048
AhdI GACNNNNNGTC 1 cut(s) 304
AjiI CACGTC 2 cut(s) 493, 513
AjnI CCWGG 1 cut(s) 1029
AleI CACNNNNGTG 1 cut(s) 779
AloI GAACNNNNNNTCC 4 cut(s) 269, 301, 896, 928
AluBI AGCT 7 cut(s) 115, 313, 367, 556, 847, 864, 1240
AluI AGCT 7 cut(s) 115, 313, 367, 556, 847, 864, 1240
Alw21I GWGCWC 5 cut(s) 117, 126, 651, 999, 1242
Alw44I GTGCAC 1 cut(s) 647
AlwI GGATC 1 cut(s) 1238
Ama87I CYCGRG 1 cut(s) 1004
Aor13HI TCCGGA 1 cut(s) 858
AoxI GGCC 8 cut(s) 243, 530, 564, 693, 715, 834, 1027, 1038
ApaLI GTGCAC 1 cut(s) 647
ApeKI GCWGC 3 cut(s) 394, 864, 867
ApoI RAATTY 2 cut(s) 47, 83
AscI GGCGCGCC 1 cut(s) 918
AsiGI ACCGGT 1 cut(s) 20
AspLEI GCGC 5 cut(s) 65, 250, 872, 920, 922
AspS9I GGNCC 6 cut(s) 158, 519, 531, 1008, 1027, 1103
AsuC2I CCSGG 3 cut(s) 1005, 1006, 1136
AsuHPI GGTGA 6 cut(s) 172, 220, 235, 595, 634, 673
AsuII TTCGAA 1 cut(s) 971
AsuNHI GCTAGC 1 cut(s) 751
AvaI CYCGRG 1 cut(s) 1004
AvaII GGWCC 4 cut(s) 158, 519, 1008, 1103
BaeGI GKGCMC 2 cut(s) 651, 786
BalI TGGCCA 1 cut(s) 1040
BanI GGYRCC 3 cut(s) 231, 781, 880
BanII GRGCYC 2 cut(s) 117, 1242
BauI CACGAG 1 cut(s) 523
BbrPI CACGTG 2 cut(s) 646, 768
BbsI GAAGAC 2 cut(s) 417, 993
Bbv12I GWGCWC 5 cut(s) 117, 126, 651, 999, 1242
BbvI GCAGC 3 cut(s) 381, 851, 879
BccI CCATC 3 cut(s) 797, 1049, 1199
BceAI ACGGC 7 cut(s) 258, 285, 339, 402, 619, 1006, 1072
BciT130I CCWGG 1 cut(s) 1031
BciVI GTATCC 1 cut(s) 1011
BclI TGATCA 1 cut(s) 318
BcnI CCSGG 3 cut(s) 1005, 1006, 1136
BfaI CTAG 1 cut(s) 752
BfmI CTRYAG 1 cut(s) 865
BfuI GTATCC 1 cut(s) 1011
BglI GCCNNNNNGGC 1 cut(s) 692
BlpI GCTNAGC 1 cut(s) 368
Bme1390I CCNGG 4 cut(s) 1005, 1006, 1031, 1136
Bme18I GGWCC 4 cut(s) 158, 519, 1008, 1103
BmeRI GACNNNNNGTC 1 cut(s) 304
BmeT110I CYCGRG 1 cut(s) 1004
BmgBI CACGTC 2 cut(s) 493, 513
BmgT120I GGNCC 6 cut(s) 158, 519, 531, 1008, 1027, 1103
BmiI GGNNCC 7 cut(s) 233, 520, 543, 783, 882, 1009, 1172
BmrFI CCNGG 4 cut(s) 1005, 1006, 1031, 1136
BmsI GCATC 2 cut(s) 162, 381
BmtI GCTAGC 1 cut(s) 755
BpiI GAAGAC 2 cut(s) 417, 993
BplI GAGNNNNNCTC 2 cut(s) 599, 631
BpmI CTGGAG 1 cut(s) 950
Bpu1102I GCTNAGC 1 cut(s) 368
Bpu14I TTCGAA 1 cut(s) 971
BpuMI CCSGG 3 cut(s) 1005, 1006, 1136
BsaAI YACGTR 3 cut(s) 382, 646, 768
BsaHI GRCGYC 3 cut(s) 389, 1059, 1077
BsaJI CCNNGG 6 cut(s) 42, 240, 534, 1004, 1023, 1249
BsaWI WCCGGW 4 cut(s) 20, 585, 849, 858
BsaXI ACNNNNNCTCC 6 cut(s) 269, 299, 489, 519, 896, 926
Bsc4I CCNNNNNNNGG 8 cut(s) 109, 241, 514, 528, 535, 713, 989, 1036
Bse118I RCCGGY 7 cut(s) 20, 440, 528, 662, 713, 832, 922
BseAI TCCGGA 1 cut(s) 858
BseBI CCWGG 1 cut(s) 1031
BseDI CCNNGG 6 cut(s) 42, 240, 534, 1004, 1023, 1249
BseGI GGATG 3 cut(s) 372, 582, 789
BseLI CCNNNNNNNGG 8 cut(s) 109, 241, 514, 528, 535, 713, 989, 1036
BseMII CTCAG 2 cut(s) 382, 662
BsePI GCGCGC 1 cut(s) 918
BseRI GAGGAG 2 cut(s) 290, 920
BseSI GKGCMC 2 cut(s) 651, 786
BseX3I CGGCCG 3 cut(s) 243, 693, 715
BseXI GCAGC 3 cut(s) 381, 851, 879
BseYI CCCAGC 1 cut(s) 552
Bsh1236I CGCG 8 cut(s) 63, 248, 260, 698, 872, 898, 920, 941
Bsh1285I CGRYCG 5 cut(s) 246, 590, 696, 718, 811
BshFI GGCC 8 cut(s) 245, 532, 566, 695, 717, 836, 1029, 1040
BshNI GGYRCC 3 cut(s) 231, 781, 880
BshTI ACCGGT 1 cut(s) 20
BsiEI CGRYCG 5 cut(s) 246, 590, 696, 718, 811
BsiHKAI GWGCWC 5 cut(s) 117, 126, 651, 999, 1242
BsiHKCI CYCGRG 1 cut(s) 1004
BslFI GGGAC 1 cut(s) 532
BslI CCNNNNNNNGG 8 cut(s) 109, 241, 514, 528, 535, 713, 989, 1036
BsmFI GGGAC 1 cut(s) 532
BsnI GGCC 8 cut(s) 245, 532, 566, 695, 717, 836, 1029, 1040
BsoBI CYCGRG 1 cut(s) 1004
Bsp119I TTCGAA 1 cut(s) 971
Bsp1286I GDGCHC 6 cut(s) 117, 126, 651, 786, 999, 1242
Bsp13I TCCGGA 1 cut(s) 858
Bsp143I GATC 4 cut(s) 318, 624, 798, 1230
Bsp1720I GCTNAGC 1 cut(s) 368
BspANI GGCC 8 cut(s) 245, 532, 566, 695, 717, 836, 1029, 1040
BspCNI CTCAG 2 cut(s) 381, 663
BspEI TCCGGA 1 cut(s) 858
BspFNI CGCG 8 cut(s) 63, 248, 260, 698, 872, 898, 920, 941
BspLI GGNNCC 7 cut(s) 233, 520, 543, 783, 882, 1009, 1172
BspMAI CTGCAG 1 cut(s) 869
BspOI GCTAGC 1 cut(s) 755
BspPI GGATC 1 cut(s) 1238
BspT104I TTCGAA 1 cut(s) 971
BspT107I GGYRCC 3 cut(s) 231, 781, 880
BsrBI CCGCTC 1 cut(s) 692
BsrFI RCCGGY 7 cut(s) 20, 440, 528, 662, 713, 832, 922
BssAI RCCGGY 7 cut(s) 20, 440, 528, 662, 713, 832, 922
BssECI CCNNGG 6 cut(s) 42, 240, 534, 1004, 1023, 1249
BssHII GCGCGC 1 cut(s) 918
BssMI GATC 4 cut(s) 318, 624, 798, 1230
BssNI GRCGYC 3 cut(s) 389, 1059, 1077
BssSI CACGAG 1 cut(s) 523
BssT1I CCWWGG 1 cut(s) 534
Bst2BI CACGAG 1 cut(s) 523
Bst2UI CCWGG 1 cut(s) 1031
Bst4CI ACNGT 5 cut(s) 172, 622, 812, 885, 1250
Bst6I CTCTTC 1 cut(s) 506
BstACI GRCGYC 3 cut(s) 389, 1059, 1077
BstBAI YACGTR 3 cut(s) 382, 646, 768
BstBI TTCGAA 1 cut(s) 971
BstDEI CTNAG 2 cut(s) 368, 671
BstDSI CCRYGG 3 cut(s) 240, 1023, 1249
BstF5I GGATG 3 cut(s) 372, 582, 789
BstFNI CGCG 8 cut(s) 63, 248, 260, 698, 872, 898, 920, 941
BstHHI GCGC 5 cut(s) 65, 250, 872, 920, 922
BstKTI GATC 4 cut(s) 321, 627, 801, 1233
BstMBI GATC 4 cut(s) 318, 624, 798, 1230
BstMCI CGRYCG 5 cut(s) 246, 590, 696, 718, 811
BstMWI GCNNNNNNNGC 9 cut(s) 121, 245, 269, 353, 692, 761, 878, 1092, 1148
BstNI CCWGG 1 cut(s) 1031
BstNSI RCATGY 2 cut(s) 751, 1280
BstSCI CCNGG 4 cut(s) 1003, 1004, 1029, 1134
BstSFI CTRYAG 1 cut(s) 865
BstSLI GKGCMC 2 cut(s) 651, 786
BstUI CGCG 8 cut(s) 63, 248, 260, 698, 872, 898, 920, 941
BstV1I GCAGC 3 cut(s) 381, 851, 879
BstV2I GAAGAC 2 cut(s) 417, 993
BstZI CGGCCG 3 cut(s) 243, 693, 715
BsuI GTATCC 1 cut(s) 1011
BsuRI GGCC 8 cut(s) 245, 532, 566, 695, 717, 836, 1029, 1040
BtgI CCRYGG 3 cut(s) 240, 1023, 1249
BtgZI GCGATG 1 cut(s) 956
BtrI CACGTC 2 cut(s) 493, 513
BtsCI GGATG 3 cut(s) 372, 582, 789
BtsI GCAGTG 1 cut(s) 774
BtsIMutI CAGTG 1 cut(s) 774
CciNI GCGGCCGC 1 cut(s) 693
CfoI GCGC 5 cut(s) 65, 250, 872, 920, 922
Cfr10I RCCGGY 7 cut(s) 20, 440, 528, 662, 713, 832, 922
Cfr13I GGNCC 6 cut(s) 158, 519, 531, 1008, 1027, 1103
Cfr9I CCCGGG 1 cut(s) 1004
CseI GACGC 5 cut(s) 378, 709, 861, 904, 1048
Csp6I GTAC 3 cut(s) 265, 772, 1210
CspAI ACCGGT 1 cut(s) 20
CviAII CATG 3 cut(s) 98, 748, 1277
CviQI GTAC 3 cut(s) 265, 772, 1210
DdeI CTNAG 2 cut(s) 368, 671
DpnI GATC 4 cut(s) 320, 626, 800, 1232
DpnII GATC 4 cut(s) 318, 624, 798, 1230
DraIII CACNNNGTG 1 cut(s) 1222
DrdI GACNNNNNNGTC 1 cut(s) 739
DriI GACNNNNNGTC 1 cut(s) 304
DseDI GACNNNNNNGTC 1 cut(s) 739
EaeI YGGCCR 4 cut(s) 243, 693, 715, 1038
EagI CGGCCG 3 cut(s) 243, 693, 715
Eam1104I CTCTTC 1 cut(s) 506
Eam1105I GACNNNNNGTC 1 cut(s) 304
EarI CTCTTC 1 cut(s) 506
EciI GGCGGA 3 cut(s) 287, 341, 394
Ecl136II GAGCTC 2 cut(s) 115, 1240
EclXI CGGCCG 3 cut(s) 243, 693, 715
Eco130I CCWWGG 1 cut(s) 534
Eco24I GRGCYC 2 cut(s) 117, 1242
Eco47I GGWCC 4 cut(s) 158, 519, 1008, 1103
Eco52I CGGCCG 3 cut(s) 243, 693, 715
Eco53kI GAGCTC 2 cut(s) 115, 1240
Eco72I CACGTG 2 cut(s) 646, 768
Eco88I CYCGRG 1 cut(s) 1004
EcoICRI GAGCTC 2 cut(s) 115, 1240
EcoO109I RGGNCCY 1 cut(s) 1103
EcoRI GAATTC 1 cut(s) 47
EcoRII CCWGG 1 cut(s) 1029
EcoT14I CCWWGG 1 cut(s) 534
EcoT38I GRGCYC 2 cut(s) 117, 1242
ErhI CCWWGG 1 cut(s) 534
FaeI CATG 3 cut(s) 101, 751, 1280
FaiI YATR 7 cut(s) 99, 414, 597, 749, 757, 1125, 1278
FaqI GGGAC 1 cut(s) 532
FatI CATG 3 cut(s) 97, 747, 1276
FauI CCCGC 1 cut(s) 715
FbaI TGATCA 1 cut(s) 318
FokI GGATG 3 cut(s) 359, 569, 776
FriOI GRGCYC 2 cut(s) 117, 1242
FspBI CTAG 1 cut(s) 752
GlaI GCGC 5 cut(s) 64, 249, 871, 919, 921
GsaI CCCAGC 1 cut(s) 556
GsuI CTGGAG 1 cut(s) 950
HaeIII GGCC 8 cut(s) 245, 532, 566, 695, 717, 836, 1029, 1040
HgaI GACGC 5 cut(s) 378, 709, 861, 904, 1048
HhaI GCGC 5 cut(s) 65, 250, 872, 920, 922
Hin1I GRCGYC 3 cut(s) 389, 1059, 1077
Hin1II CATG 3 cut(s) 101, 751, 1280
Hin6I GCGC 5 cut(s) 63, 248, 870, 918, 920
HinP1I GCGC 5 cut(s) 63, 248, 870, 918, 920
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HindIII AAGCTT 1 cut(s) 311
HpaI GTTAAC 1 cut(s) 478
HphI GGTGA 6 cut(s) 172, 220, 235, 595, 634, 673
Hpy166II GTNNAC 5 cut(s) 267, 478, 649, 677, 774
Hpy188III TCNNGA 6 cut(s) 52, 164, 806, 859, 1015, 1045
Hpy8I GTNNAC 5 cut(s) 267, 478, 649, 677, 774
HpyAV CCTTC 2 cut(s) 28, 193
HpyCH4III ACNGT 5 cut(s) 172, 622, 812, 885, 1250
HpyCH4IV ACGT 7 cut(s) 381, 492, 512, 645, 734, 767, 1077
HpyCH4V TGCA 4 cut(s) 649, 867, 1095, 1216
HpyF10VI GCNNNNNNNGC 9 cut(s) 121, 245, 269, 353, 692, 761, 878, 1092, 1148
HpyF3I CTNAG 2 cut(s) 368, 671
HpySE526I ACGT 7 cut(s) 381, 492, 512, 645, 734, 767, 1077
Hsp92I GRCGYC 3 cut(s) 389, 1059, 1077
Hsp92II CATG 3 cut(s) 101, 751, 1280
HspAI GCGC 5 cut(s) 63, 248, 870, 918, 920
Kpn2I TCCGGA 1 cut(s) 858
KroI GCCGGC 4 cut(s) 440, 528, 662, 713
KroNI GCCGGC 4 cut(s) 442, 530, 664, 715
Ksp22I TGATCA 1 cut(s) 318
KspAI GTTAAC 1 cut(s) 478
Kzo9I GATC 4 cut(s) 318, 624, 798, 1230
LmnI GCTCC 6 cut(s) 112, 689, 708, 852, 861, 1237
Lsp1109I GCAGC 3 cut(s) 381, 851, 879
LweI GCATC 2 cut(s) 162, 381
MaeI CTAG 1 cut(s) 752
MaeII ACGT 7 cut(s) 381, 492, 512, 645, 734, 767, 1077
MaeIII GTNAC 4 cut(s) 382, 812, 978, 1111
MalI GATC 4 cut(s) 320, 626, 800, 1232
MbiI CCGCTC 1 cut(s) 692
MboI GATC 4 cut(s) 318, 624, 798, 1230
MboII GAAGA 4 cut(s) 43, 417, 493, 998
MhlI GDGCHC 6 cut(s) 117, 126, 651, 786, 999, 1242
MlsI TGGCCA 1 cut(s) 1040
MluCI AATT 7 cut(s) 47, 83, 137, 336, 888, 1034, 1258
MluI ACGCGT 1 cut(s) 896
MluNI TGGCCA 1 cut(s) 1040
MlyI GAGTC 8 cut(s) 293, 314, 395, 452, 608, 617, 917, 1193
MmeI TCCRAC 5 cut(s) 321, 480, 513, 591, 1208
Mox20I TGGCCA 1 cut(s) 1040
MroI TCCGGA 1 cut(s) 858
MroNI GCCGGC 4 cut(s) 440, 528, 662, 713
MscI TGGCCA 1 cut(s) 1040
MseI TTAA 5 cut(s) 255, 477, 656, 1245, 1262
MslI CAYNNNNRTG 2 cut(s) 779, 1165
Msp20I TGGCCA 1 cut(s) 1040
MspA1I CMGCKG 2 cut(s) 149, 1083
MspR9I CCNGG 4 cut(s) 1005, 1006, 1031, 1136
MvaI CCWGG 1 cut(s) 1031
MvnI CGCG 8 cut(s) 63, 248, 260, 698, 872, 898, 920, 941
MwoI GCNNNNNNNGC 9 cut(s) 121, 245, 269, 353, 692, 761, 878, 1092, 1148
NaeI GCCGGC 4 cut(s) 442, 530, 664, 715
NciI CCSGG 3 cut(s) 1005, 1006, 1136
NdeII GATC 4 cut(s) 318, 624, 798, 1230
NgoMIV GCCGGC 4 cut(s) 440, 528, 662, 713
NheI GCTAGC 1 cut(s) 751
NlaIII CATG 3 cut(s) 101, 751, 1280
NlaIV GGNNCC 7 cut(s) 233, 520, 543, 783, 882, 1009, 1172
NmeAIII GCCGAG 2 cut(s) 260, 474
NmuCI GTSAC 3 cut(s) 382, 812, 1111
NotI GCGGCCGC 1 cut(s) 693
NspI RCATGY 2 cut(s) 751, 1280
NspV TTCGAA 1 cut(s) 971
OliI CACNNNNGTG 1 cut(s) 779
PalAI GGCGCGCC 1 cut(s) 918
PauI GCGCGC 1 cut(s) 918
PciI ACATGT 1 cut(s) 1276
PcsI WCGNNNNNNNCGW 8 cut(s) 50, 162, 486, 521, 881, 1020, 1056, 1083
PdiI GCCGGC 4 cut(s) 442, 530, 664, 715
PfeI GAWTC 2 cut(s) 720, 973
PflFI GACNNNGTC 2 cut(s) 170, 388
PflMI CCANNNNNTGG 1 cut(s) 1036
PinAI ACCGGT 1 cut(s) 20
PleI GAGTC 8 cut(s) 293, 313, 395, 452, 608, 616, 916, 1193
PmaCI CACGTG 2 cut(s) 646, 768
PmlI CACGTG 2 cut(s) 646, 768
PpsI GAGTC 8 cut(s) 293, 313, 395, 452, 608, 616, 916, 1193
Ppu21I YACGTR 3 cut(s) 382, 646, 768
PpuMI RGGWCCY 1 cut(s) 1103
PscI ACATGT 1 cut(s) 1276
Psp124BI GAGCTC 2 cut(s) 117, 1242
Psp5II RGGWCCY 1 cut(s) 1103
Psp6I CCWGG 1 cut(s) 1029
PspCI CACGTG 2 cut(s) 646, 768
PspFI CCCAGC 1 cut(s) 552
PspGI CCWGG 1 cut(s) 1029
PspN4I GGNNCC 7 cut(s) 233, 520, 543, 783, 882, 1009, 1172
PspPI GGNCC 6 cut(s) 158, 519, 531, 1008, 1027, 1103
PspPPI RGGWCCY 1 cut(s) 1103
PstI CTGCAG 1 cut(s) 869
PsyI GACNNNGTC 2 cut(s) 170, 388
PteI GCGCGC 1 cut(s) 918
RsaI GTAC 3 cut(s) 266, 773, 1211
RsaNI GTAC 3 cut(s) 265, 772, 1210
RseI CAYNNNNRTG 2 cut(s) 779, 1165
SacI GAGCTC 2 cut(s) 117, 1242
SaqAI TTAA 5 cut(s) 255, 477, 656, 1245, 1262
Sau3AI GATC 4 cut(s) 318, 624, 798, 1230
Sau96I GGNCC 6 cut(s) 158, 519, 531, 1008, 1027, 1103
SchI GAGTC 8 cut(s) 293, 314, 395, 452, 608, 617, 917, 1193
ScrFI CCNGG 4 cut(s) 1005, 1006, 1031, 1136
SduI GDGCHC 6 cut(s) 117, 126, 651, 786, 999, 1242
SfaNI GCATC 2 cut(s) 162, 381
SfcI CTRYAG 1 cut(s) 865
SfuI TTCGAA 1 cut(s) 971
SgrAI CRCCGGYG 1 cut(s) 922
SgsI GGCGCGCC 1 cut(s) 918
SinI GGWCC 4 cut(s) 158, 519, 1008, 1103
SmaI CCCGGG 1 cut(s) 1006
SmiMI CAYNNNNRTG 2 cut(s) 779, 1165
Sse9I AATT 7 cut(s) 47, 83, 137, 336, 888, 1034, 1258
SspMI CTAG 1 cut(s) 752
SstI GAGCTC 2 cut(s) 117, 1242
StyD4I CCNGG 4 cut(s) 1003, 1004, 1029, 1134
StyI CCWWGG 1 cut(s) 534
TaaI ACNGT 5 cut(s) 172, 622, 812, 885, 1250
TaiI ACGT 7 cut(s) 384, 495, 515, 648, 737, 770, 1080
TaqI TCGA 9 cut(s) 87, 120, 282, 308, 617, 731, 807, 971, 1282
TaqII GACCGA 2 cut(s) 146, 580
TasI AATT 7 cut(s) 47, 83, 137, 336, 888, 1034, 1258
TfiI GAWTC 2 cut(s) 720, 973
Tru1I TTAA 5 cut(s) 255, 477, 656, 1245, 1262
Tru9I TTAA 5 cut(s) 255, 477, 656, 1245, 1262
TscAI CASTG 1 cut(s) 781
TseFI GTSAC 3 cut(s) 382, 812, 1111
TseI GCWGC 3 cut(s) 394, 864, 867
Tsp45I GTSAC 3 cut(s) 382, 812, 1111
TspGWI ACGGA 4 cut(s) 61, 962, 966, 1000
TspMI CCCGGG 1 cut(s) 1004
TspRI CASTG 1 cut(s) 781
Tth111I GACNNNGTC 2 cut(s) 170, 388
Van91I CCANNNNNTGG 1 cut(s) 1036
VneI GTGCAC 1 cut(s) 647
VpaK11BI GGWCC 4 cut(s) 158, 519, 1008, 1103
XapI RAATTY 2 cut(s) 47, 83
XceI RCATGY 2 cut(s) 751, 1280
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XmaI CCCGGG 1 cut(s) 1004
XspI CTAG 1 cut(s) 752
ZraI GACGTC 1 cut(s) 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.