FvH4_5g03710

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0017298
Physical Location & Seq
Reverse (-)
2167674 .. 2168234
561 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g03710.t1

Sequence Viewer

Length: 561 bp
ATGGGAGTTAGAAAGTCTATTGTTCTTGTGTCCTTCTTCATGTTGTTCCTTTCTTTATCTTCAGCTTACCCTAAACCAAGAAAACAGTACAAACCATGCAAACACCTAGTGCTCTTCTTCCATGACATTATCTACAATGGTAAGAACGCTGCCAATGCCACATCTGCAATTGTAGCAGCACCTCAAGGAGCCAACCTAACCATCTTGGCTTCCCAATTCCACTTCGGGAACATTGCTGTTTTCGATGACCCCATCACCCTTGACAACAATCTGCACTCAAAGCCTGTGGGCAGGGCACAAGGCATGTACCTCTATGATACAAAGAACACTTACACCGCGTGGCTTGGGTTCTCGTTTTCTTTGAACACCACAGCCTACCAGGGTAGCATAAACTTCATCGGAGCCGATCCTCTTATGAATAAAACTAGAGATATATCCGTTGTGGGTGGCACCGGAGACTTTTTCATGCACCGGGGAGTCGCAACCATCATGACAGATGCTTATGAAGGTGAAGTATACTTCAGGCTCCGGGTTGACATCAACTTTTACGAGTGTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

187

Amino Acids

20.82

Weight (kDa)

8.83

Isoelectric Point (pI)

19.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 33 - 178 3.4e-37 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017298)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g03710
malus_domestica MD06G1096500.v1.1
prunus_persica Prupe.5G111300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0196011
rosa_laevigata RLG00000004074
rosa_multiflora Rmu_sc0001640.1_g000014
rosa_roxburghii Rroxscaffold_3G00259930
rosa_rugosa Rorug07G0029000
rosa_samantha Rh7AG155600 Rh7BG157500 Rh7CG161100 Rh7DG156600
rosa_wichuraiana Rw7G013310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 449
AccI GTMKAC 1 cut(s) 516
AccII CGCG 1 cut(s) 338
AciI CCGC 1 cut(s) 336
AclWI GGATC 1 cut(s) 401
AcuI CTGAAG 2 cut(s) 45, 505
AdeI CACNNNGTG 2 cut(s) 109, 339
AfaI GTAC 2 cut(s) 89, 308
AgsI TTSAA 1 cut(s) 364
AjnI CCWGG 1 cut(s) 378
AluBI AGCT 1 cut(s) 65
AluI AGCT 1 cut(s) 65
Alw21I GWGCWC 1 cut(s) 114
Alw26I GTCTC 1 cut(s) 450
AlwI GGATC 1 cut(s) 401
ApeKI GCWGC 2 cut(s) 149, 176
AsuC2I CCSGG 2 cut(s) 473, 530
AsuHPI GGTGA 2 cut(s) 247, 521
BaeGI GKGCMC 1 cut(s) 298
BanI GGYRCC 1 cut(s) 449
Bbv12I GWGCWC 1 cut(s) 114
BbvI GCAGC 2 cut(s) 136, 188
BccI CCATC 3 cut(s) 209, 260, 494
BciT130I CCWGG 1 cut(s) 380
BcnI CCSGG 2 cut(s) 473, 530
BcoDI GTCTC 1 cut(s) 450
BfaI CTAG 2 cut(s) 107, 426
BisI GCNGC 2 cut(s) 150, 177
BlsI GCNGC 2 cut(s) 151, 178
Bme1390I CCNGG 3 cut(s) 380, 473, 530
BmiI GGNNCC 4 cut(s) 190, 403, 451, 527
BmrFI CCNGG 3 cut(s) 380, 473, 530
BmsI GCATC 1 cut(s) 487
BpuEI CTTGAG 1 cut(s) 168
BpuMI CCSGG 2 cut(s) 473, 530
BsaJI CCNNGG 2 cut(s) 379, 472
BsaWI WCCGGW 1 cut(s) 452
Bse3DI GCAATG 1 cut(s) 231
BseBI CCWGG 1 cut(s) 380
BseDI CCNNGG 2 cut(s) 379, 472
BseMI GCAATG 1 cut(s) 231
BseSI GKGCMC 1 cut(s) 298
BseXI GCAGC 2 cut(s) 136, 188
BsgI GTGCAG 1 cut(s) 257
Bsh1236I CGCG 1 cut(s) 338
BshNI GGYRCC 1 cut(s) 449
BsiHKAI GWGCWC 1 cut(s) 114
BsiSI CCGG 3 cut(s) 453, 472, 529
BsmAI GTCTC 1 cut(s) 450
Bsp1286I GDGCHC 2 cut(s) 114, 298
Bsp143I GATC 1 cut(s) 406
BspACI CCGC 1 cut(s) 336
BspFNI CGCG 1 cut(s) 338
BspHI TCATGA 1 cut(s) 489
BspLI GGNNCC 4 cut(s) 190, 403, 451, 527
BspPI GGATC 1 cut(s) 401
BspQI GCTCTTC 1 cut(s) 119
BspT107I GGYRCC 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 231
BssECI CCNNGG 2 cut(s) 379, 472
BssMI GATC 1 cut(s) 406
BssNAI GTATAC 1 cut(s) 517
Bst1107I GTATAC 1 cut(s) 517
Bst2UI CCWGG 1 cut(s) 380
Bst4CI ACNGT 1 cut(s) 87
Bst6I CTCTTC 1 cut(s) 119
BstFNI CGCG 1 cut(s) 338
BstKTI GATC 1 cut(s) 409
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 1 cut(s) 406
BstMWI GCNNNNNNNGC 4 cut(s) 155, 164, 173, 280
BstNI CCWGG 1 cut(s) 380
BstNSI RCATGY 1 cut(s) 307
BstSCI CCNGG 3 cut(s) 378, 471, 528
BstSLI GKGCMC 1 cut(s) 298
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 2 cut(s) 136, 188
BstZ17I GTATAC 1 cut(s) 517
CciI TCATGA 1 cut(s) 489
Csp6I GTAC 2 cut(s) 88, 307
CspCI CAANNNNNGTGG 2 cut(s) 267, 302
CviAII CATG 6 cut(s) 40, 96, 122, 304, 466, 490
CviJI RGCY 8 cut(s) 65, 191, 209, 283, 343, 374, 404, 526
CviKI_1 RGCY 8 cut(s) 65, 191, 209, 283, 343, 374, 404, 526
CviQI GTAC 2 cut(s) 88, 307
DpnI GATC 1 cut(s) 408
DpnII GATC 1 cut(s) 406
DraIII CACNNNGTG 2 cut(s) 109, 339
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
Eco57I CTGAAG 2 cut(s) 45, 505
EcoRII CCWGG 1 cut(s) 378
FaeI CATG 6 cut(s) 43, 99, 125, 307, 469, 493
FatI CATG 6 cut(s) 39, 95, 121, 303, 465, 489
FblI GTMKAC 1 cut(s) 516
Fnu4HI GCNGC 2 cut(s) 150, 177
Fsp4HI GCNGC 2 cut(s) 150, 177
FspBI CTAG 2 cut(s) 107, 426
GluI GCNGC 2 cut(s) 150, 177
HapII CCGG 3 cut(s) 453, 472, 529
Hin1II CATG 6 cut(s) 43, 99, 125, 307, 469, 493
HincII GTYRAC 1 cut(s) 535
HindII GTYRAC 1 cut(s) 535
HinfI GANTC 1 cut(s) 477
HpaII CCGG 3 cut(s) 453, 472, 529
HphI GGTGA 2 cut(s) 247, 521
Hpy166II GTNNAC 2 cut(s) 517, 535
Hpy188I TCNGA 1 cut(s) 401
Hpy188III TCNNGA 2 cut(s) 226, 490
Hpy8I GTNNAC 2 cut(s) 517, 535
HpyAV CCTTC 2 cut(s) 43, 500
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4V TGCA 4 cut(s) 99, 167, 274, 469
HpyF10VI GCNNNNNNNGC 4 cut(s) 155, 164, 173, 280
Hsp92II CATG 6 cut(s) 43, 99, 125, 307, 469, 493
Kzo9I GATC 1 cut(s) 406
LguI GCTCTTC 1 cut(s) 119
LmnI GCTCC 3 cut(s) 188, 401, 531
LpnPI CCDG 8 cut(s) 277, 297, 365, 392, 466, 485, 508, 542
Lsp1109I GCAGC 2 cut(s) 136, 188
LweI GCATC 1 cut(s) 487
MaeI CTAG 2 cut(s) 107, 426
MalI GATC 1 cut(s) 408
MboI GATC 1 cut(s) 406
MboII GAAGA 4 cut(s) 28, 51, 106, 109
MfeI CAATTG 1 cut(s) 168
MhlI GDGCHC 2 cut(s) 114, 298
MluCI AATT 2 cut(s) 168, 215
MlyI GAGTC 1 cut(s) 486
MnlI CCTC 3 cut(s) 192, 320, 420
MspI CCGG 3 cut(s) 453, 472, 529
MspR9I CCNGG 3 cut(s) 380, 473, 530
MunI CAATTG 1 cut(s) 168
MvaI CCWGG 1 cut(s) 380
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 4 cut(s) 155, 164, 173, 280
NciI CCSGG 2 cut(s) 473, 530
NdeII GATC 1 cut(s) 406
NlaIII CATG 6 cut(s) 43, 99, 125, 307, 469, 493
NlaIV GGNNCC 4 cut(s) 190, 403, 451, 527
NspI RCATGY 1 cut(s) 307
PagI TCATGA 1 cut(s) 489
PciSI GCTCTTC 1 cut(s) 119
PkrI GCNGC 2 cut(s) 151, 178
PleI GAGTC 1 cut(s) 485
PpsI GAGTC 1 cut(s) 485
Psp6I CCWGG 1 cut(s) 378
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 4 cut(s) 190, 403, 451, 527
RsaI GTAC 2 cut(s) 89, 308
RsaNI GTAC 2 cut(s) 88, 307
SapI GCTCTTC 1 cut(s) 119
SatI GCNGC 2 cut(s) 150, 177
Sau3AI GATC 1 cut(s) 406
SchI GAGTC 1 cut(s) 486
ScrFI CCNGG 3 cut(s) 380, 473, 530
SduI GDGCHC 2 cut(s) 114, 298
SetI ASST 6 cut(s) 67, 108, 184, 198, 312, 511
SfaNI GCATC 1 cut(s) 487
SmlI CTYRAG 1 cut(s) 183
SmoI CTYRAG 1 cut(s) 183
Sse9I AATT 2 cut(s) 168, 215
SsiI CCGC 1 cut(s) 336
SspMI CTAG 2 cut(s) 107, 426
StyD4I CCNGG 3 cut(s) 378, 471, 528
TaaI ACNGT 1 cut(s) 87
TaqI TCGA 1 cut(s) 243
TasI AATT 2 cut(s) 168, 215
TatI WGTACW 1 cut(s) 87
TseI GCWGC 2 cut(s) 149, 176
TspDTI ATGAA 5 cut(s) 28, 385, 431, 454, 519
TspGWI ACGGA 1 cut(s) 427
XceI RCATGY 1 cut(s) 307
XmiI GTMKAC 1 cut(s) 516
XspI CTAG 2 cut(s) 107, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.