MD06G1096500.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0017298
Physical Location & Seq
Reverse (-)
23073869 .. 23074426
558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1096500.v1.1.491

Sequence Viewer

Length: 558 bp
ATGGAAGCAAGAAGTTCCTCGGTTCTAATATTCTTCTTCATGTTCCTCGCTATATCTTCAGCCTTCCCAAAACACAAAGACAAGTACAAACCATGCAAGCACTTGGTGCTATTCTTCCATGACATTATCTACAATGGAAAAAATGCTGCCAATGCTACATCTGCGATTGTGGCAGCCCCTCAAGGATCCAACCTAACCATCTTGGCACCGAATTTTCATTTCGGGAACATAGCGGTTTTCGATGACCCCATTACCCTCGACAACAATCTGCACTCCAAGCCTGTGGGCAGGGCACAAGGGATGTACATGTATGATACAAAGAACACATTCACTGCTTGGCTTGGCTTCTCATTTTATCTAAACAGCACAGACTACCAAGGCACAATAAATTTCATCGGAGCCGACCCCATTTTGATTAAATATAGGGAGATATCCGTCGTGGGTGGCACCGGGGACTTTTTCATGCACCGGGGAGTTGCAACCATAGACACGGATTCATATGAAGGTGAAGTCTACTTCAGGCTCAAGGTGGATATCAAGTTCTATGAGTGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

186

Amino Acids

20.9

Weight (kDa)

6.58

Isoelectric Point (pI)

30.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 32 - 177 2e-37 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017298)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g03710
malus_domestica MD06G1096500.v1.1
prunus_persica Prupe.5G111300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0196011
rosa_laevigata RLG00000004074
rosa_multiflora Rmu_sc0001640.1_g000014
rosa_roxburghii Rroxscaffold_3G00259930
rosa_rugosa Rorug07G0029000
rosa_samantha Rh7AG155600 Rh7BG157500 Rh7CG161100 Rh7DG156600
rosa_wichuraiana Rw7G013310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 205, 446
AccI GTMKAC 1 cut(s) 513
AciI CCGC 1 cut(s) 233
AclWI GGATC 2 cut(s) 180, 193
AcsI RAATTY 2 cut(s) 211, 388
AcuI CTGAAG 2 cut(s) 42, 502
AdeI CACNNNGTG 1 cut(s) 106
AfaI GTAC 2 cut(s) 86, 305
AflIII ACRYGT 1 cut(s) 306
AloI GAACNNNNNNTCC 2 cut(s) 524, 556
AlwI GGATC 2 cut(s) 180, 193
ApeKI GCWGC 2 cut(s) 146, 173
ApoI RAATTY 2 cut(s) 211, 388
Asp700I GAANNNNTTC 1 cut(s) 326
AsuC2I CCSGG 2 cut(s) 451, 470
AsuHPI GGTGA 1 cut(s) 518
BaeGI GKGCMC 1 cut(s) 295
BamHI GGATCC 1 cut(s) 185
BanI GGYRCC 2 cut(s) 205, 446
BbvI GCAGC 2 cut(s) 133, 185
BccI CCATC 1 cut(s) 206
BcnI CCSGG 2 cut(s) 451, 470
BisI GCNGC 2 cut(s) 147, 174
BlsI GCNGC 2 cut(s) 148, 175
Bme1390I CCNGG 2 cut(s) 451, 470
BmiI GGNNCC 4 cut(s) 187, 207, 400, 448
BmrFI CCNGG 2 cut(s) 451, 470
BpuEI CTTGAG 2 cut(s) 165, 509
BpuMI CCSGG 2 cut(s) 451, 470
BsaJI CCNNGG 4 cut(s) 18, 376, 450, 469
BseDI CCNNGG 4 cut(s) 18, 376, 450, 469
BseGI GGATG 1 cut(s) 306
BseSI GKGCMC 1 cut(s) 295
BseXI GCAGC 2 cut(s) 133, 185
BsgI GTGCAG 1 cut(s) 254
BshNI GGYRCC 2 cut(s) 205, 446
BsiSI CCGG 2 cut(s) 450, 469
BslFI GGGAC 1 cut(s) 467
BsmFI GGGAC 1 cut(s) 467
Bsp1286I GDGCHC 1 cut(s) 295
Bsp1407I TGTACA 1 cut(s) 303
Bsp143I GATC 1 cut(s) 185
BspACI CCGC 1 cut(s) 233
BspLI GGNNCC 4 cut(s) 187, 207, 400, 448
BspPI GGATC 2 cut(s) 180, 193
BspT107I GGYRCC 2 cut(s) 205, 446
BsrGI TGTACA 1 cut(s) 303
BssECI CCNNGG 4 cut(s) 18, 376, 450, 469
BssMI GATC 1 cut(s) 185
BssT1I CCWWGG 1 cut(s) 376
BstAPI GCANNNNNTGC 1 cut(s) 106
BstAUI TGTACA 1 cut(s) 303
BstC8I GCNNGC 1 cut(s) 98
BstF5I GGATG 1 cut(s) 306
BstKTI GATC 1 cut(s) 188
BstMBI GATC 1 cut(s) 185
BstMWI GCNNNNNNNGC 5 cut(s) 106, 152, 161, 170, 277
BstNSI RCATGY 1 cut(s) 310
BstSCI CCNGG 2 cut(s) 449, 468
BstSLI GKGCMC 1 cut(s) 295
BstV1I GCAGC 2 cut(s) 133, 185
BstX2I RGATCY 1 cut(s) 185
BstXI CCANNNNNNTGG 1 cut(s) 283
BstYI RGATCY 1 cut(s) 185
BtsCI GGATG 1 cut(s) 306
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 1 cut(s) 330
Cac8I GCNNGC 1 cut(s) 98
Csp6I GTAC 2 cut(s) 85, 304
CviAII CATG 5 cut(s) 40, 93, 119, 307, 463
CviJI RGCY 7 cut(s) 62, 176, 280, 340, 345, 401, 523
CviKI_1 RGCY 7 cut(s) 62, 176, 280, 340, 345, 401, 523
CviQI GTAC 2 cut(s) 85, 304
DpnI GATC 1 cut(s) 187
DpnII GATC 1 cut(s) 185
DraIII CACNNNGTG 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 376
Eco32I GATATC 2 cut(s) 432, 535
Eco57I CTGAAG 2 cut(s) 42, 502
EcoRV GATATC 2 cut(s) 432, 535
EcoT14I CCWWGG 1 cut(s) 376
ErhI CCWWGG 1 cut(s) 376
FaeI CATG 5 cut(s) 43, 96, 122, 310, 466
FaqI GGGAC 1 cut(s) 467
FatI CATG 5 cut(s) 39, 92, 118, 306, 462
FauNDI CATATG 1 cut(s) 499
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 2 cut(s) 147, 174
FokI GGATG 1 cut(s) 313
Fsp4HI GCNGC 2 cut(s) 147, 174
GluI GCNGC 2 cut(s) 147, 174
HapII CCGG 2 cut(s) 450, 469
Hin1II CATG 5 cut(s) 43, 96, 122, 310, 466
HinfI GANTC 1 cut(s) 494
HpaII CCGG 2 cut(s) 450, 469
HphI GGTGA 1 cut(s) 518
Hpy166II GTNNAC 1 cut(s) 514
Hpy188I TCNGA 1 cut(s) 398
Hpy188III TCNNGA 1 cut(s) 223
Hpy8I GTNNAC 1 cut(s) 514
Hpy99I CGWCG 1 cut(s) 440
HpyAV CCTTC 2 cut(s) 73, 497
HpyCH4V TGCA 4 cut(s) 96, 271, 466, 479
HpyF10VI GCNNNNNNNGC 5 cut(s) 106, 152, 161, 170, 277
Hsp92II CATG 5 cut(s) 43, 96, 122, 310, 466
Kzo9I GATC 1 cut(s) 185
LmnI GCTCC 1 cut(s) 398
LpnPI CCDG 5 cut(s) 274, 294, 463, 482, 505
Lsp1109I GCAGC 2 cut(s) 133, 185
MalI GATC 1 cut(s) 187
MboI GATC 1 cut(s) 185
MboII GAAGA 4 cut(s) 25, 28, 48, 106
MflI RGATCY 1 cut(s) 185
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 2 cut(s) 211, 388
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 4 cut(s) 28, 56, 189, 266
MroXI GAANNNNTTC 1 cut(s) 326
MseI TTAA 1 cut(s) 417
MspI CCGG 2 cut(s) 450, 469
MspR9I CCNGG 2 cut(s) 451, 470
MwoI GCNNNNNNNGC 5 cut(s) 106, 152, 161, 170, 277
NciI CCSGG 2 cut(s) 451, 470
NdeI CATATG 1 cut(s) 499
NdeII GATC 1 cut(s) 185
NlaIII CATG 5 cut(s) 43, 96, 122, 310, 466
NlaIV GGNNCC 4 cut(s) 187, 207, 400, 448
NspI RCATGY 1 cut(s) 310
PciI ACATGT 1 cut(s) 306
PdmI GAANNNNTTC 1 cut(s) 326
PfeI GAWTC 1 cut(s) 494
PkrI GCNGC 2 cut(s) 148, 175
PscI ACATGT 1 cut(s) 306
PspN4I GGNNCC 4 cut(s) 187, 207, 400, 448
PsuI RGATCY 1 cut(s) 185
RsaI GTAC 2 cut(s) 86, 305
RsaNI GTAC 2 cut(s) 85, 304
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 2 cut(s) 147, 174
Sau3AI GATC 1 cut(s) 185
ScrFI CCNGG 2 cut(s) 451, 470
SduI GDGCHC 1 cut(s) 295
SetI ASST 3 cut(s) 195, 508, 531
SmlI CTYRAG 2 cut(s) 180, 524
SmoI CTYRAG 2 cut(s) 180, 524
Sse9I AATT 2 cut(s) 211, 388
SsiI CCGC 1 cut(s) 233
SspI AATATT 1 cut(s) 30
StyD4I CCNGG 2 cut(s) 449, 468
StyI CCWWGG 1 cut(s) 376
TaqI TCGA 2 cut(s) 240, 258
TasI AATT 2 cut(s) 211, 388
TatI WGTACW 2 cut(s) 84, 303
TfiI GAWTC 1 cut(s) 494
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TscAI CASTG 1 cut(s) 337
TseI GCWGC 2 cut(s) 146, 173
TspDTI ATGAA 6 cut(s) 28, 206, 382, 451, 486, 516
TspGWI ACGGA 2 cut(s) 424, 506
TspRI CASTG 1 cut(s) 337
XapI RAATTY 2 cut(s) 211, 388
XceI RCATGY 1 cut(s) 310
XmiI GTMKAC 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.