RLG00000004074

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017298
Physical Location & Seq
Reverse (-)
55873316 .. 55873873
558 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004074

Sequence Viewer

Length: 558 bp
ATGGGAGTTAGAAATTCTATTGTTCTTGTTTCCTTCATGTTGCTCCTTGCTTTATCTTCAGCCTTCCCTAAACATAAAAAACAGTACAAACCATGCAAAAACCTAGTGCTGTACTTCCATGACATTATTTACAATGGCAAGAACGCTGCCAATGCCACATCTGCAATTGTAGCAGCACCTCAAGGAGCCAACCTAACCATCTTGGCTCCCCAATTCCACTTCGGGAACATTGCTGTTTTCGATGACCCCATCACCCTTGACAACAATCTGCACTCAAAGCCGGTGGGCAGGGCACAAGGCATGTACATCTATGATACAAAGAACACTTACACCGCGTGGCTTGGGTTTACGTTTTCTTTGAACACCACAGCCTACCAGGGTACCATAAACTTCATCGGAGCTGATCCCCTTATGATAAAAACTAGAGATATATCCGTCGTGGGTGGCACCGGAGACTTTTTCATGCACCGGGGAGTAGCAACAATCATGACGGATGCGTATGAAGGTGAAGTATACTTCAGGCTCCGGGTTGACATCAACTTTTACGAGTGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

186

Amino Acids

20.64

Weight (kDa)

8.49

Isoelectric Point (pI)

22.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 32 - 177 1e-36 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017298)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g03710
malus_domestica MD06G1096500.v1.1
prunus_persica Prupe.5G111300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0196011
rosa_laevigata RLG00000004074
rosa_multiflora Rmu_sc0001640.1_g000014
rosa_roxburghii Rroxscaffold_3G00259930
rosa_rugosa Rorug07G0029000
rosa_samantha Rh7AG155600 Rh7BG157500 Rh7CG161100 Rh7DG156600
rosa_wichuraiana Rw7G013310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 380
AccB1I GGYRCC 2 cut(s) 380, 446
AccI GTMKAC 1 cut(s) 513
AccII CGCG 1 cut(s) 335
AciI CCGC 1 cut(s) 333
AclWI GGATC 1 cut(s) 398
AcsI RAATTY 1 cut(s) 13
AcuI CTGAAG 2 cut(s) 42, 502
AdeI CACNNNGTG 1 cut(s) 336
AfaI GTAC 4 cut(s) 86, 113, 305, 382
AgsI TTSAA 1 cut(s) 361
AjnI CCWGG 1 cut(s) 375
AluBI AGCT 1 cut(s) 401
AluI AGCT 1 cut(s) 401
Alw26I GTCTC 1 cut(s) 447
AlwI GGATC 1 cut(s) 398
ApeKI GCWGC 2 cut(s) 146, 173
ApoI RAATTY 1 cut(s) 13
Asp718I GGTACC 1 cut(s) 380
AsuC2I CCSGG 2 cut(s) 470, 527
AsuHPI GGTGA 2 cut(s) 244, 518
BaeGI GKGCMC 1 cut(s) 295
BaeI ACNNNNGTAYC 2 cut(s) 364, 397
BanI GGYRCC 2 cut(s) 380, 446
BbvI GCAGC 2 cut(s) 133, 185
BccI CCATC 2 cut(s) 206, 257
BciT130I CCWGG 1 cut(s) 377
BcnI CCSGG 2 cut(s) 470, 527
BcoDI GTCTC 1 cut(s) 447
BfaI CTAG 2 cut(s) 104, 423
BisI GCNGC 2 cut(s) 147, 174
BlsI GCNGC 2 cut(s) 148, 175
Bme1390I CCNGG 3 cut(s) 377, 470, 527
BmiI GGNNCC 5 cut(s) 187, 207, 382, 448, 524
BmrFI CCNGG 3 cut(s) 377, 470, 527
BmsI GCATC 1 cut(s) 484
BpuEI CTTGAG 1 cut(s) 165
BpuMI CCSGG 2 cut(s) 470, 527
BsaJI CCNNGG 2 cut(s) 376, 469
BsaWI WCCGGW 1 cut(s) 449
Bse118I RCCGGY 1 cut(s) 280
Bse3DI GCAATG 1 cut(s) 228
BseBI CCWGG 1 cut(s) 377
BseDI CCNNGG 2 cut(s) 376, 469
BseGI GGATG 1 cut(s) 499
BseMI GCAATG 1 cut(s) 228
BseSI GKGCMC 1 cut(s) 295
BseXI GCAGC 2 cut(s) 133, 185
BsgI GTGCAG 1 cut(s) 254
Bsh1236I CGCG 1 cut(s) 335
BshNI GGYRCC 2 cut(s) 380, 446
BsiSI CCGG 4 cut(s) 281, 450, 469, 526
BsmAI GTCTC 1 cut(s) 447
Bsp1286I GDGCHC 1 cut(s) 295
Bsp1407I TGTACA 1 cut(s) 303
Bsp143I GATC 1 cut(s) 403
BspACI CCGC 1 cut(s) 333
BspFNI CGCG 1 cut(s) 335
BspHI TCATGA 1 cut(s) 486
BspLI GGNNCC 5 cut(s) 187, 207, 382, 448, 524
BspPI GGATC 1 cut(s) 398
BspT107I GGYRCC 2 cut(s) 380, 446
BsrDI GCAATG 1 cut(s) 228
BsrFI RCCGGY 1 cut(s) 280
BsrGI TGTACA 1 cut(s) 303
BssAI RCCGGY 1 cut(s) 280
BssECI CCNNGG 2 cut(s) 376, 469
BssMI GATC 1 cut(s) 403
BssNAI GTATAC 1 cut(s) 514
Bst1107I GTATAC 1 cut(s) 514
Bst2UI CCWGG 1 cut(s) 377
Bst4CI ACNGT 1 cut(s) 84
BstAUI TGTACA 1 cut(s) 303
BstF5I GGATG 1 cut(s) 499
BstFNI CGCG 1 cut(s) 335
BstKTI GATC 1 cut(s) 406
BstMAI GTCTC 1 cut(s) 447
BstMBI GATC 1 cut(s) 403
BstMWI GCNNNNNNNGC 4 cut(s) 152, 161, 170, 277
BstNI CCWGG 1 cut(s) 377
BstNSI RCATGY 1 cut(s) 304
BstSCI CCNGG 3 cut(s) 375, 468, 525
BstSLI GKGCMC 1 cut(s) 295
BstUI CGCG 1 cut(s) 335
BstV1I GCAGC 2 cut(s) 133, 185
BstZ17I GTATAC 1 cut(s) 514
BtsCI GGATG 1 cut(s) 499
CciI TCATGA 1 cut(s) 486
Cfr10I RCCGGY 1 cut(s) 280
Csp6I GTAC 4 cut(s) 85, 112, 304, 381
CspCI CAANNNNNGTGG 2 cut(s) 264, 299
CviAII CATG 6 cut(s) 37, 93, 119, 301, 463, 487
CviJI RGCY 8 cut(s) 62, 188, 206, 280, 340, 371, 401, 523
CviKI_1 RGCY 8 cut(s) 62, 188, 206, 280, 340, 371, 401, 523
CviQI GTAC 4 cut(s) 85, 112, 304, 381
DpnI GATC 1 cut(s) 405
DpnII GATC 1 cut(s) 403
DraIII CACNNNGTG 1 cut(s) 336
Eco57I CTGAAG 2 cut(s) 42, 502
EcoRII CCWGG 1 cut(s) 375
FaeI CATG 6 cut(s) 40, 96, 122, 304, 466, 490
FatI CATG 6 cut(s) 36, 92, 118, 300, 462, 486
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 2 cut(s) 147, 174
FokI GGATG 1 cut(s) 506
Fsp4HI GCNGC 2 cut(s) 147, 174
FspBI CTAG 2 cut(s) 104, 423
GluI GCNGC 2 cut(s) 147, 174
HapII CCGG 4 cut(s) 281, 450, 469, 526
Hin1II CATG 6 cut(s) 40, 96, 122, 304, 466, 490
HincII GTYRAC 1 cut(s) 532
HindII GTYRAC 1 cut(s) 532
HpaII CCGG 4 cut(s) 281, 450, 469, 526
HphI GGTGA 2 cut(s) 244, 518
Hpy166II GTNNAC 3 cut(s) 348, 514, 532
Hpy188I TCNGA 1 cut(s) 398
Hpy188III TCNNGA 2 cut(s) 223, 487
Hpy8I GTNNAC 3 cut(s) 348, 514, 532
Hpy99I CGWCG 1 cut(s) 440
HpyAV CCTTC 3 cut(s) 43, 73, 497
HpyCH4III ACNGT 1 cut(s) 84
HpyCH4IV ACGT 1 cut(s) 350
HpyCH4V TGCA 4 cut(s) 96, 164, 271, 466
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 161, 170, 277
HpySE526I ACGT 1 cut(s) 350
Hsp92II CATG 6 cut(s) 40, 96, 122, 304, 466, 490
KpnI GGTACC 1 cut(s) 384
Kzo9I GATC 1 cut(s) 403
LmnI GCTCC 5 cut(s) 48, 185, 211, 398, 528
LpnPI CCDG 8 cut(s) 274, 294, 362, 389, 463, 482, 505, 539
Lsp1109I GCAGC 2 cut(s) 133, 185
LweI GCATC 1 cut(s) 484
MaeI CTAG 2 cut(s) 104, 423
MaeII ACGT 1 cut(s) 350
MalI GATC 1 cut(s) 405
MboI GATC 1 cut(s) 403
MboII GAAGA 1 cut(s) 48
MfeI CAATTG 1 cut(s) 165
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 3 cut(s) 13, 165, 212
MnlI CCTC 1 cut(s) 189
MspI CCGG 4 cut(s) 281, 450, 469, 526
MspR9I CCNGG 3 cut(s) 377, 470, 527
MunI CAATTG 1 cut(s) 165
MvaI CCWGG 1 cut(s) 377
MvnI CGCG 1 cut(s) 335
MwoI GCNNNNNNNGC 4 cut(s) 152, 161, 170, 277
NciI CCSGG 2 cut(s) 470, 527
NdeII GATC 1 cut(s) 403
NlaIII CATG 6 cut(s) 40, 96, 122, 304, 466, 490
NlaIV GGNNCC 5 cut(s) 187, 207, 382, 448, 524
NspI RCATGY 1 cut(s) 304
PagI TCATGA 1 cut(s) 486
PkrI GCNGC 2 cut(s) 148, 175
Psp6I CCWGG 1 cut(s) 375
PspGI CCWGG 1 cut(s) 375
PspN4I GGNNCC 5 cut(s) 187, 207, 382, 448, 524
RsaI GTAC 4 cut(s) 86, 113, 305, 382
RsaNI GTAC 4 cut(s) 85, 112, 304, 381
SatI GCNGC 2 cut(s) 147, 174
Sau3AI GATC 1 cut(s) 403
ScrFI CCNGG 3 cut(s) 377, 470, 527
SduI GDGCHC 1 cut(s) 295
SetI ASST 6 cut(s) 105, 181, 195, 353, 403, 508
SfaNI GCATC 1 cut(s) 484
SmlI CTYRAG 1 cut(s) 180
SmoI CTYRAG 1 cut(s) 180
Sse9I AATT 3 cut(s) 13, 165, 212
SsiI CCGC 1 cut(s) 333
SspMI CTAG 2 cut(s) 104, 423
StyD4I CCNGG 3 cut(s) 375, 468, 525
TaaI ACNGT 1 cut(s) 84
TaiI ACGT 1 cut(s) 353
TaqI TCGA 1 cut(s) 240
TasI AATT 3 cut(s) 13, 165, 212
TatI WGTACW 3 cut(s) 84, 111, 303
TseI GCWGC 2 cut(s) 146, 173
TspDTI ATGAA 4 cut(s) 25, 382, 451, 516
TspGWI ACGGA 2 cut(s) 424, 506
XapI RAATTY 1 cut(s) 13
XceI RCATGY 1 cut(s) 304
XmiI GTMKAC 1 cut(s) 513
XspI CTAG 2 cut(s) 104, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.