Prupe.5G111300_v2.0.a1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0017298
Physical Location & Seq
Reverse (-)
11554343 .. 11554955
613 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G111300.1

Sequence Viewer

Length: 558 bp
ATGGCAGCGAGAAAAACCTCTGTTCTTCTTTTCTTCTTCATGTTCCTTGCTTTATGCTCAGCATTCCCAAGAAATAAAAACAAGTACAAGCCATGCAAGCACTTGGTGCTATTCTTCCATGACATTATTTACAATGGTAAAAATGCTGCCAATGCTACAGCAGCAATCGTAGCAGCTCCTCAAGGAGCCAATCTAACCATCTTGGCACCCCAATTTCATTTCGGAAACATTGCGGTTTTCGACGATCCCATCACCCTTGACAACAATCTGCACTCCAAGCCTGTTGGGAGGGCTCAAGGGATGTACATTTATGATACAAAGAACACATTCACTTCTTGGCTTGGCTTCTCATTTGCTCTGAACAGCACAGACTACCAAGGCAGCATAAATTTCATTGGGGTCGATCCCATTTTGATTAAAACTAGGGACATATCTGTGGTCGGTGGCACCGGAGACTTTTTCATGCACCGGGGAATCGCAACCATCAACACCGACGCATATGAAGGTGAAGTCTACTTCAGGCTCAAGGTGGATATCAAGTTTTATGAGTGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

186

Amino Acids

20.74

Weight (kDa)

8.73

Isoelectric Point (pI)

22.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017298)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g03710
malus_domestica MD06G1096500.v1.1
prunus_persica Prupe.5G111300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0196011
rosa_laevigata RLG00000004074
rosa_multiflora Rmu_sc0001640.1_g000014
rosa_roxburghii Rroxscaffold_3G00259930
rosa_rugosa Rorug07G0029000
rosa_samantha Rh7AG155600 Rh7BG157500 Rh7CG161100 Rh7DG156600
rosa_wichuraiana Rw7G013310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 205, 446
AccI GTMKAC 1 cut(s) 513
AciI CCGC 1 cut(s) 233
AclWI GGATC 2 cut(s) 239, 398
AcsI RAATTY 1 cut(s) 388
AcuI CTGAAG 1 cut(s) 502
AdeI CACNNNGTG 1 cut(s) 106
AfaI GTAC 2 cut(s) 86, 305
AluBI AGCT 1 cut(s) 176
AluI AGCT 1 cut(s) 176
Alw26I GTCTC 1 cut(s) 447
AlwI GGATC 2 cut(s) 239, 398
ApeKI GCWGC 5 cut(s) 5, 146, 161, 173, 381
ApoI RAATTY 1 cut(s) 388
Asp700I GAANNNNTTC 1 cut(s) 326
AsuC2I CCSGG 1 cut(s) 470
AsuHPI GGTGA 2 cut(s) 244, 518
BanI GGYRCC 2 cut(s) 205, 446
BanII GRGCYC 1 cut(s) 295
BbvI GCAGC 5 cut(s) 17, 133, 173, 185, 393
BccI CCATC 3 cut(s) 206, 257, 491
BcnI CCSGG 1 cut(s) 470
BcoDI GTCTC 1 cut(s) 447
BfaI CTAG 1 cut(s) 423
BfmI CTRYAG 1 cut(s) 156
BisI GCNGC 5 cut(s) 6, 147, 162, 174, 382
BlpI GCTNAGC 1 cut(s) 58
BlsI GCNGC 5 cut(s) 7, 148, 163, 175, 383
Bme1390I CCNGG 1 cut(s) 470
BmiI GGNNCC 3 cut(s) 187, 207, 448
BmrFI CCNGG 1 cut(s) 470
Bpu1102I GCTNAGC 1 cut(s) 58
BpuEI CTTGAG 3 cut(s) 165, 279, 509
BpuMI CCSGG 1 cut(s) 470
BsaJI CCNNGG 2 cut(s) 376, 469
BsaWI WCCGGW 1 cut(s) 449
Bse3DI GCAATG 1 cut(s) 228
BseDI CCNNGG 2 cut(s) 376, 469
BseGI GGATG 1 cut(s) 306
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 1 cut(s) 72
BseRI GAGGAG 1 cut(s) 168
BseXI GCAGC 5 cut(s) 17, 133, 173, 185, 393
BsgI GTGCAG 1 cut(s) 254
BshNI GGYRCC 2 cut(s) 205, 446
BsiSI CCGG 2 cut(s) 450, 469
BslFI GGGAC 1 cut(s) 440
BsmAI GTCTC 1 cut(s) 447
BsmFI GGGAC 1 cut(s) 440
BsmI GAATGC 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 295
Bsp1407I TGTACA 1 cut(s) 303
Bsp143I GATC 2 cut(s) 244, 403
Bsp1720I GCTNAGC 1 cut(s) 58
BspACI CCGC 1 cut(s) 233
BspCNI CTCAG 1 cut(s) 71
BspLI GGNNCC 3 cut(s) 187, 207, 448
BspPI GGATC 2 cut(s) 239, 398
BspT107I GGYRCC 2 cut(s) 205, 446
BsrDI GCAATG 1 cut(s) 228
BsrGI TGTACA 1 cut(s) 303
BssECI CCNNGG 2 cut(s) 376, 469
BssMI GATC 2 cut(s) 244, 403
BssT1I CCWWGG 1 cut(s) 376
BstAPI GCANNNNNTGC 1 cut(s) 106
BstAUI TGTACA 1 cut(s) 303
BstC8I GCNNGC 1 cut(s) 98
BstDEI CTNAG 1 cut(s) 58
BstF5I GGATG 1 cut(s) 306
BstKTI GATC 2 cut(s) 247, 406
BstMAI GTCTC 1 cut(s) 447
BstMBI GATC 2 cut(s) 244, 403
BstMWI GCNNNNNNNGC 6 cut(s) 97, 106, 152, 161, 170, 277
BstSCI CCNGG 1 cut(s) 468
BstSFI CTRYAG 1 cut(s) 156
BstV1I GCAGC 5 cut(s) 17, 133, 173, 185, 393
BtsCI GGATG 1 cut(s) 306
Cac8I GCNNGC 1 cut(s) 98
CseI GACGC 1 cut(s) 503
Csp6I GTAC 2 cut(s) 85, 304
CviAII CATG 4 cut(s) 40, 93, 119, 463
CviJI RGCY 8 cut(s) 91, 176, 188, 280, 293, 340, 345, 523
CviKI_1 RGCY 8 cut(s) 91, 176, 188, 280, 293, 340, 345, 523
CviQI GTAC 2 cut(s) 85, 304
DdeI CTNAG 1 cut(s) 58
DpnI GATC 2 cut(s) 246, 405
DpnII GATC 2 cut(s) 244, 403
DraIII CACNNNGTG 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 376
Eco24I GRGCYC 1 cut(s) 295
Eco32I GATATC 1 cut(s) 535
Eco57I CTGAAG 1 cut(s) 502
EcoRV GATATC 1 cut(s) 535
EcoT14I CCWWGG 1 cut(s) 376
EcoT38I GRGCYC 1 cut(s) 295
ErhI CCWWGG 1 cut(s) 376
FaeI CATG 4 cut(s) 43, 96, 122, 466
FaqI GGGAC 1 cut(s) 440
FatI CATG 4 cut(s) 39, 92, 118, 462
FauNDI CATATG 1 cut(s) 499
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 5 cut(s) 6, 147, 162, 174, 382
FokI GGATG 1 cut(s) 313
FriOI GRGCYC 1 cut(s) 295
Fsp4HI GCNGC 5 cut(s) 6, 147, 162, 174, 382
FspBI CTAG 1 cut(s) 423
GluI GCNGC 5 cut(s) 6, 147, 162, 174, 382
HapII CCGG 2 cut(s) 450, 469
HgaI GACGC 1 cut(s) 503
Hin1II CATG 4 cut(s) 43, 96, 122, 466
HinfI GANTC 1 cut(s) 474
HpaII CCGG 2 cut(s) 450, 469
HphI GGTGA 2 cut(s) 244, 518
Hpy166II GTNNAC 1 cut(s) 514
Hpy188I TCNGA 2 cut(s) 224, 360
Hpy8I GTNNAC 1 cut(s) 514
Hpy99I CGWCG 2 cut(s) 245, 497
HpyAV CCTTC 1 cut(s) 497
HpyCH4V TGCA 3 cut(s) 96, 271, 466
HpyF10VI GCNNNNNNNGC 6 cut(s) 97, 106, 152, 161, 170, 277
HpyF3I CTNAG 1 cut(s) 58
Hsp92II CATG 4 cut(s) 43, 96, 122, 466
Kzo9I GATC 2 cut(s) 244, 403
LmnI GCTCC 2 cut(s) 181, 185
LpnPI CCDG 4 cut(s) 294, 463, 482, 505
Lsp1109I GCAGC 5 cut(s) 17, 133, 173, 185, 393
MaeI CTAG 1 cut(s) 423
MalI GATC 2 cut(s) 246, 405
MboI GATC 2 cut(s) 244, 403
MboII GAAGA 4 cut(s) 17, 25, 28, 106
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 2 cut(s) 212, 388
MnlI CCTC 3 cut(s) 28, 189, 282
MroXI GAANNNNTTC 1 cut(s) 326
MseI TTAA 1 cut(s) 417
MslI CAYNNNNRTG 1 cut(s) 434
MspI CCGG 2 cut(s) 450, 469
MspR9I CCNGG 1 cut(s) 470
Mva1269I GAATGC 1 cut(s) 62
MwoI GCNNNNNNNGC 6 cut(s) 97, 106, 152, 161, 170, 277
NciI CCSGG 1 cut(s) 470
NdeI CATATG 1 cut(s) 499
NdeII GATC 2 cut(s) 244, 403
NlaIII CATG 4 cut(s) 43, 96, 122, 466
NlaIV GGNNCC 3 cut(s) 187, 207, 448
PctI GAATGC 1 cut(s) 62
PdmI GAANNNNTTC 1 cut(s) 326
PfeI GAWTC 1 cut(s) 474
PkrI GCNGC 5 cut(s) 7, 148, 163, 175, 383
PspN4I GGNNCC 3 cut(s) 187, 207, 448
RsaI GTAC 2 cut(s) 86, 305
RsaNI GTAC 2 cut(s) 85, 304
RseI CAYNNNNRTG 1 cut(s) 434
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 5 cut(s) 6, 147, 162, 174, 382
Sau3AI GATC 2 cut(s) 244, 403
ScrFI CCNGG 1 cut(s) 470
SduI GDGCHC 1 cut(s) 295
SetI ASST 4 cut(s) 20, 178, 508, 531
SfcI CTRYAG 1 cut(s) 156
SmiMI CAYNNNNRTG 1 cut(s) 434
SmlI CTYRAG 3 cut(s) 180, 294, 524
SmoI CTYRAG 3 cut(s) 180, 294, 524
Sse9I AATT 2 cut(s) 212, 388
SsiI CCGC 1 cut(s) 233
SspMI CTAG 1 cut(s) 423
StyD4I CCNGG 1 cut(s) 468
StyI CCWWGG 1 cut(s) 376
TaqI TCGA 2 cut(s) 240, 402
TasI AATT 2 cut(s) 212, 388
TatI WGTACW 2 cut(s) 84, 303
TfiI GAWTC 1 cut(s) 474
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TseI GCWGC 5 cut(s) 5, 146, 161, 173, 381
TspDTI ATGAA 5 cut(s) 28, 206, 382, 451, 516
XapI RAATTY 1 cut(s) 388
XmiI GTMKAC 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 326
XspI CTAG 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.