Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g03710 |
| malus_domestica | MD06G1096500.v1.1 |
| prunus_persica | Prupe.5G111300_v2.0.a1 |
| rosa_chinensis | RchiOBHm_Chr7g0196011 |
| rosa_laevigata | RLG00000004074 |
| rosa_multiflora | Rmu_sc0001640.1_g000014 |
| rosa_roxburghii | Rroxscaffold_3G00259930 |
| rosa_rugosa | Rorug07G0029000 |
| rosa_samantha | Rh7AG155600 Rh7BG157500 Rh7CG161100 Rh7DG156600 |
| rosa_wichuraiana | Rw7G013310 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 144, 1347, 1626 |
| Acc36I | ACCTGC | 1 cut(s) | 1405 |
| AccI | GTMKAC | 1 cut(s) | 1419 |
| AciI | CCGC | 3 cut(s) | 36, 132, 305 |
| AclWI | GGATC | 4 cut(s) | 1037, 1383, 1444, 1457 |
| AcoI | YGGCCR | 4 cut(s) | 302, 340, 978, 1926 |
| AcsI | RAATTY | 4 cut(s) | 568, 602, 1349, 1648 |
| AcuI | CTGAAG | 2 cut(s) | 1267, 1614 |
| AfaI | GTAC | 2 cut(s) | 397, 1053 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 47, 220, 349, 976, 1500, 1776 |
| AflIII | ACRYGT | 2 cut(s) | 1942, 1959 |
| AhlI | ACTAGT | 1 cut(s) | 398 |
| AjnI | CCWGG | 1 cut(s) | 1734 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1932, 1964 |
| AluBI | AGCT | 3 cut(s) | 192, 1154, 2008 |
| AluI | AGCT | 3 cut(s) | 192, 1154, 2008 |
| Alw26I | GTCTC | 2 cut(s) | 8, 62 |
| AlwI | GGATC | 4 cut(s) | 1037, 1383, 1444, 1457 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 122, 1677 |
| Ama87I | CYCGRG | 1 cut(s) | 1808 |
| AoxI | GGCC | 5 cut(s) | 302, 340, 351, 978, 1926 |
| ApeKI | GCWGC | 3 cut(s) | 846, 956, 1126 |
| ApoI | RAATTY | 4 cut(s) | 568, 602, 1349, 1648 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1886 |
| AspS9I | GGNCC | 2 cut(s) | 82, 351 |
| AsuC2I | CCSGG | 1 cut(s) | 344 |
| AsuHPI | GGTGA | 6 cut(s) | 87, 140, 377, 455, 604, 1559 |
| AsuNHI | GCTAGC | 1 cut(s) | 1154 |
| AvaI | CYCGRG | 1 cut(s) | 1808 |
| AvaII | GGWCC | 1 cut(s) | 82 |
| BalI | TGGCCA | 2 cut(s) | 980, 1928 |
| BamHI | GGATCC | 1 cut(s) | 1449 |
| BbsI | GAAGAC | 1 cut(s) | 1230 |
| BbvI | GCAGC | 3 cut(s) | 858, 943, 1138 |
| BccI | CCATC | 6 cut(s) | 121, 434, 968, 1002, 1010, 1191 |
| BceAI | ACGGC | 2 cut(s) | 164, 338 |
| BciT130I | CCWGG | 1 cut(s) | 1736 |
| BclI | TGATCA | 1 cut(s) | 771 |
| BcnI | CCSGG | 1 cut(s) | 344 |
| BcoDI | GTCTC | 2 cut(s) | 8, 62 |
| BcuI | ACTAGT | 1 cut(s) | 398 |
| BfaI | CTAG | 3 cut(s) | 399, 1155, 1203 |
| BfmI | CTRYAG | 2 cut(s) | 1420, 1497 |
| BfuAI | ACCTGC | 1 cut(s) | 1405 |
| BisI | GCNGC | 4 cut(s) | 305, 847, 957, 1127 |
| BlsI | GCNGC | 4 cut(s) | 306, 848, 958, 1128 |
| Bme1390I | CCNGG | 2 cut(s) | 344, 1736 |
| Bme18I | GGWCC | 1 cut(s) | 82 |
| BmeT110I | CYCGRG | 1 cut(s) | 1808 |
| BmgT120I | GGNCC | 2 cut(s) | 82, 351 |
| BmiI | GGNNCC | 2 cut(s) | 84, 1451 |
| BmrFI | CCNGG | 2 cut(s) | 344, 1736 |
| BmsI | GCATC | 6 cut(s) | 618, 997, 1167, 1198, 1729, 1824 |
| BmtI | GCTAGC | 1 cut(s) | 1158 |
| BpiI | GAAGAC | 1 cut(s) | 1230 |
| BpmI | CTGGAG | 1 cut(s) | 714 |
| BpuEI | CTTGAG | 1 cut(s) | 84 |
| BpuMI | CCSGG | 1 cut(s) | 344 |
| BsaAI | YACGTR | 2 cut(s) | 229, 1544 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1380 |
| BsaI | GGTCTC | 1 cut(s) | 8 |
| BsaJI | CCNNGG | 2 cut(s) | 343, 354 |
| BsaWI | WCCGGW | 1 cut(s) | 150 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 59, 89, 482, 512, 975, 1005 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 47, 220, 349, 976, 1500, 1776 |
| Bse1I | ACTGG | 3 cut(s) | 595, 1076, 1610 |
| Bse3DI | GCAATG | 1 cut(s) | 848 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1380 |
| BseBI | CCWGG | 1 cut(s) | 1736 |
| BseDI | CCNNGG | 2 cut(s) | 343, 354 |
| BseGI | GGATG | 8 cut(s) | 32, 445, 867, 960, 1202, 1213, 1569, 1744 |
| BseJI | GATNNNNATC | 1 cut(s) | 1380 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 47, 220, 349, 976, 1500, 1776 |
| BseMI | GCAATG | 1 cut(s) | 848 |
| BseMII | CTCAG | 1 cut(s) | 1833 |
| BseNI | ACTGG | 3 cut(s) | 595, 1076, 1610 |
| BseRI | GAGGAG | 1 cut(s) | 654 |
| BseX3I | CGGCCG | 1 cut(s) | 340 |
| BseXI | GCAGC | 3 cut(s) | 858, 943, 1138 |
| BsgI | GTGCAG | 2 cut(s) | 841, 1433 |
| Bsh1285I | CGRYCG | 1 cut(s) | 343 |
| BshFI | GGCC | 5 cut(s) | 304, 342, 353, 980, 1928 |
| BsiEI | CGRYCG | 1 cut(s) | 343 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1808 |
| BsiSI | CCGG | 2 cut(s) | 151, 343 |
| BslFI | GGGAC | 2 cut(s) | 68, 517 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 47, 220, 349, 976, 1500, 1776 |
| BsmAI | GTCTC | 2 cut(s) | 8, 62 |
| BsmBI | CGTCTC | 1 cut(s) | 62 |
| BsmFI | GGGAC | 2 cut(s) | 68, 517 |
| BsmI | GAATGC | 3 cut(s) | 233, 410, 958 |
| BsnI | GGCC | 5 cut(s) | 304, 342, 353, 980, 1928 |
| Bso31I | GGTCTC | 1 cut(s) | 8 |
| BsoBI | CYCGRG | 1 cut(s) | 1808 |
| Bsp1407I | TGTACA | 1 cut(s) | 1051 |
| Bsp143I | GATC | 8 cut(s) | 771, 882, 1008, 1029, 1130, 1375, 1449, 1729 |
| BspACI | CCGC | 3 cut(s) | 36, 132, 305 |
| BspANI | GGCC | 5 cut(s) | 304, 342, 353, 980, 1928 |
| BspCNI | CTCAG | 1 cut(s) | 1834 |
| BspLI | GGNNCC | 2 cut(s) | 84, 1451 |
| BspMI | ACCTGC | 1 cut(s) | 1405 |
| BspOI | GCTAGC | 1 cut(s) | 1158 |
| BspPI | GGATC | 4 cut(s) | 1037, 1383, 1444, 1457 |
| BspTNI | GGTCTC | 1 cut(s) | 8 |
| BsrDI | GCAATG | 1 cut(s) | 848 |
| BsrGI | TGTACA | 1 cut(s) | 1051 |
| BsrI | ACTGG | 3 cut(s) | 595, 1076, 1610 |
| BssECI | CCNNGG | 2 cut(s) | 343, 354 |
| BssMI | GATC | 8 cut(s) | 771, 882, 1008, 1029, 1130, 1375, 1449, 1729 |
| Bst2UI | CCWGG | 1 cut(s) | 1736 |
| Bst4CI | ACNGT | 6 cut(s) | 124, 1172, 1368, 1424, 1517, 2024 |
| Bst6I | CTCTTC | 4 cut(s) | 206, 756, 1292, 1590 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 953 |
| BstAUI | TGTACA | 1 cut(s) | 1051 |
| BstBAI | YACGTR | 2 cut(s) | 229, 1544 |
| BstC8I | GCNNGC | 3 cut(s) | 1156, 1464, 1826 |
| BstDEI | CTNAG | 4 cut(s) | 1162, 1797, 1842, 1991 |
| BstDSI | CCRYGG | 1 cut(s) | 354 |
| BstF5I | GGATG | 8 cut(s) | 32, 445, 867, 960, 1202, 1213, 1569, 1744 |
| BstKTI | GATC | 8 cut(s) | 774, 885, 1011, 1032, 1133, 1378, 1452, 1732 |
| BstMAI | GTCTC | 2 cut(s) | 8, 62 |
| BstMBI | GATC | 8 cut(s) | 771, 882, 1008, 1029, 1130, 1375, 1449, 1729 |
| BstMCI | CGRYCG | 1 cut(s) | 343 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 855, 953, 1834, 2005 |
| BstNI | CCWGG | 1 cut(s) | 1736 |
| BstSCI | CCNGG | 2 cut(s) | 342, 1734 |
| BstSFI | CTRYAG | 2 cut(s) | 1420, 1497 |
| BstV1I | GCAGC | 3 cut(s) | 858, 943, 1138 |
| BstV2I | GAAGAC | 1 cut(s) | 1230 |
| BstX2I | RGATCY | 2 cut(s) | 1029, 1449 |
| BstYI | RGATCY | 2 cut(s) | 1029, 1449 |
| BstZI | CGGCCG | 1 cut(s) | 340 |
| BsuRI | GGCC | 5 cut(s) | 304, 342, 353, 980, 1928 |
| BtgI | CCRYGG | 1 cut(s) | 354 |
| BtsCI | GGATG | 8 cut(s) | 32, 445, 867, 960, 1202, 1213, 1569, 1744 |
| BtsI | GCAGTG | 2 cut(s) | 774, 1675 |
| BtsIMutI | CAGTG | 4 cut(s) | 774, 1083, 1603, 1675 |
| BveI | ACCTGC | 1 cut(s) | 1405 |
| Cac8I | GCNNGC | 3 cut(s) | 1156, 1464, 1826 |
| CaiI | CAGNNNCTG | 2 cut(s) | 122, 1677 |
| Cfr13I | GGNCC | 2 cut(s) | 82, 351 |
| Csp6I | GTAC | 2 cut(s) | 396, 1052 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 861, 896 |
| CviAII | CATG | 8 cut(s) | 287, 393, 787, 931, 948, 1440, 1635, 1776 |
| CviQI | GTAC | 2 cut(s) | 396, 1052 |
| DdeI | CTNAG | 4 cut(s) | 1162, 1797, 1842, 1991 |
| DpnI | GATC | 8 cut(s) | 773, 884, 1010, 1031, 1132, 1377, 1451, 1731 |
| DpnII | GATC | 8 cut(s) | 771, 882, 1008, 1029, 1130, 1375, 1449, 1729 |
| DraI | TTTAAA | 1 cut(s) | 480 |
| EaeI | YGGCCR | 4 cut(s) | 302, 340, 978, 1926 |
| EagI | CGGCCG | 1 cut(s) | 340 |
| Eam1104I | CTCTTC | 4 cut(s) | 206, 756, 1292, 1590 |
| EarI | CTCTTC | 4 cut(s) | 206, 756, 1292, 1590 |
| EciI | GGCGGA | 1 cut(s) | 121 |
| EclXI | CGGCCG | 1 cut(s) | 340 |
| Eco31I | GGTCTC | 1 cut(s) | 8 |
| Eco32I | GATATC | 1 cut(s) | 892 |
| Eco47I | GGWCC | 1 cut(s) | 82 |
| Eco52I | CGGCCG | 1 cut(s) | 340 |
| Eco57I | CTGAAG | 2 cut(s) | 1267, 1614 |
| Eco88I | CYCGRG | 1 cut(s) | 1808 |
| EcoRII | CCWGG | 1 cut(s) | 1734 |
| EcoRV | GATATC | 1 cut(s) | 892 |
| EcoT22I | ATGCAT | 1 cut(s) | 6 |
| Esp3I | CGTCTC | 1 cut(s) | 62 |
| FaeI | CATG | 8 cut(s) | 290, 396, 790, 934, 951, 1443, 1638, 1779 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1256, 1288 |
| FaqI | GGGAC | 2 cut(s) | 68, 517 |
| FatI | CATG | 8 cut(s) | 286, 392, 786, 930, 947, 1439, 1634, 1775 |
| FbaI | TGATCA | 1 cut(s) | 771 |
| FblI | GTMKAC | 1 cut(s) | 1419 |
| Fnu4HI | GCNGC | 4 cut(s) | 305, 847, 957, 1127 |
| FokI | GGATG | 8 cut(s) | 19, 452, 854, 947, 1209, 1220, 1576, 1751 |
| Fsp4HI | GCNGC | 4 cut(s) | 305, 847, 957, 1127 |
| FspBI | CTAG | 3 cut(s) | 399, 1155, 1203 |
| GluI | GCNGC | 4 cut(s) | 305, 847, 957, 1127 |
| GsuI | CTGGAG | 1 cut(s) | 714 |
| HaeIII | GGCC | 5 cut(s) | 304, 342, 353, 980, 1928 |
| HapII | CCGG | 2 cut(s) | 151, 343 |
| Hin1II | CATG | 8 cut(s) | 290, 396, 790, 934, 951, 1443, 1638, 1779 |
| HincII | GTYRAC | 3 cut(s) | 909, 1336, 1513 |
| HindII | GTYRAC | 3 cut(s) | 909, 1336, 1513 |
| HpaI | GTTAAC | 1 cut(s) | 1336 |
| HpaII | CCGG | 2 cut(s) | 151, 343 |
| HphI | GGTGA | 6 cut(s) | 87, 140, 377, 455, 604, 1559 |
| Hpy166II | GTNNAC | 6 cut(s) | 685, 909, 1293, 1336, 1420, 1513 |
| Hpy188I | TCNGA | 5 cut(s) | 865, 1063, 1386, 1408, 1843 |
| Hpy188III | TCNNGA | 7 cut(s) | 599, 655, 731, 1006, 1194, 1261, 1294 |
| Hpy8I | GTNNAC | 6 cut(s) | 685, 909, 1293, 1336, 1420, 1513 |
| Hpy99I | CGWCG | 1 cut(s) | 1343 |
| HpyAV | CCTTC | 8 cut(s) | 463, 515, 629, 639, 1291, 1589, 1747, 1991 |
| HpyCH4III | ACNGT | 6 cut(s) | 124, 1172, 1368, 1424, 1517, 2024 |
| HpyCH4IV | ACGT | 7 cut(s) | 228, 580, 617, 1543, 1915, 1942, 1961 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 855, 953, 1834, 2005 |
| HpyF3I | CTNAG | 4 cut(s) | 1162, 1797, 1842, 1991 |
| HpySE526I | ACGT | 7 cut(s) | 228, 580, 617, 1543, 1915, 1942, 1961 |
| Hsp92II | CATG | 8 cut(s) | 290, 396, 790, 934, 951, 1443, 1638, 1779 |
| Ksp22I | TGATCA | 1 cut(s) | 771 |
| KspAI | GTTAAC | 1 cut(s) | 1336 |
| Kzo9I | GATC | 8 cut(s) | 771, 882, 1008, 1029, 1130, 1375, 1449, 1729 |
| Lsp1109I | GCAGC | 3 cut(s) | 858, 943, 1138 |
| LweI | GCATC | 6 cut(s) | 618, 997, 1167, 1198, 1729, 1824 |
| MaeI | CTAG | 3 cut(s) | 399, 1155, 1203 |
| MaeII | ACGT | 7 cut(s) | 228, 580, 617, 1543, 1915, 1942, 1961 |
| MaeIII | GTNAC | 8 cut(s) | 93, 241, 268, 448, 716, 1166, 1396, 2024 |
| MalI | GATC | 8 cut(s) | 773, 884, 1010, 1031, 1132, 1377, 1451, 1731 |
| MboI | GATC | 8 cut(s) | 771, 882, 1008, 1029, 1130, 1375, 1449, 1729 |
| MfeI | CAATTG | 1 cut(s) | 974 |
| MflI | RGATCY | 2 cut(s) | 1029, 1449 |
| MlsI | TGGCCA | 2 cut(s) | 980, 1928 |
| MluNI | TGGCCA | 2 cut(s) | 980, 1928 |
| MlyI | GAGTC | 3 cut(s) | 689, 721, 921 |
| MmeI | TCCRAC | 2 cut(s) | 601, 1268 |
| Mox20I | TGGCCA | 2 cut(s) | 980, 1928 |
| Mph1103I | ATGCAT | 1 cut(s) | 6 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1886 |
| MscI | TGGCCA | 2 cut(s) | 980, 1928 |
| MseI | TTAA | 8 cut(s) | 323, 384, 479, 713, 1122, 1335, 1473, 1964 |
| Msp20I | TGGCCA | 2 cut(s) | 980, 1928 |
| MspI | CCGG | 2 cut(s) | 151, 343 |
| MspR9I | CCNGG | 2 cut(s) | 344, 1736 |
| MunI | CAATTG | 1 cut(s) | 974 |
| Mva1269I | GAATGC | 3 cut(s) | 233, 410, 958 |
| MvaI | CCWGG | 1 cut(s) | 1736 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 855, 953, 1834, 2005 |
| NciI | CCSGG | 1 cut(s) | 344 |
| NdeII | GATC | 8 cut(s) | 771, 882, 1008, 1029, 1130, 1375, 1449, 1729 |
| NheI | GCTAGC | 1 cut(s) | 1154 |
| NlaIII | CATG | 8 cut(s) | 290, 396, 790, 934, 951, 1443, 1638, 1779 |
| NlaIV | GGNNCC | 2 cut(s) | 84, 1451 |
| NmuCI | GTSAC | 3 cut(s) | 93, 241, 268 |
| NsiI | ATGCAT | 1 cut(s) | 6 |
| PctI | GAATGC | 3 cut(s) | 233, 410, 958 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1886 |
| PfeI | GAWTC | 7 cut(s) | 470, 1240, 1267, 1317, 1328, 1719, 1772 |
| PflFI | GACNNNGTC | 1 cut(s) | 73 |
| PfoI | TCCNGGA | 1 cut(s) | 1734 |
| PkrI | GCNGC | 4 cut(s) | 306, 848, 958, 1128 |
| PleI | GAGTC | 3 cut(s) | 689, 721, 921 |
| PpsI | GAGTC | 3 cut(s) | 689, 721, 921 |
| Ppu21I | YACGTR | 2 cut(s) | 229, 1544 |
| PsiI | TTATAA | 3 cut(s) | 144, 1347, 1626 |
| Psp6I | CCWGG | 1 cut(s) | 1734 |
| PspGI | CCWGG | 1 cut(s) | 1734 |
| PspN4I | GGNNCC | 2 cut(s) | 84, 1451 |
| PspPI | GGNCC | 2 cut(s) | 82, 351 |
| PstNI | CAGNNNCTG | 2 cut(s) | 122, 1677 |
| PsuI | RGATCY | 2 cut(s) | 1029, 1449 |
| PsyI | GACNNNGTC | 1 cut(s) | 73 |
| RsaI | GTAC | 2 cut(s) | 397, 1053 |
| RsaNI | GTAC | 2 cut(s) | 396, 1052 |
| SaqAI | TTAA | 8 cut(s) | 323, 384, 479, 713, 1122, 1335, 1473, 1964 |
| SatI | GCNGC | 4 cut(s) | 305, 847, 957, 1127 |
| Sau3AI | GATC | 8 cut(s) | 771, 882, 1008, 1029, 1130, 1375, 1449, 1729 |
| Sau96I | GGNCC | 2 cut(s) | 82, 351 |
| SchI | GAGTC | 3 cut(s) | 689, 721, 921 |
| ScrFI | CCNGG | 2 cut(s) | 344, 1736 |
| SfaNI | GCATC | 6 cut(s) | 618, 997, 1167, 1198, 1729, 1824 |
| SfcI | CTRYAG | 2 cut(s) | 1420, 1497 |
| SinI | GGWCC | 1 cut(s) | 82 |
| SmlI | CTYRAG | 1 cut(s) | 99 |
| SmoI | CTYRAG | 1 cut(s) | 99 |
| SpeI | ACTAGT | 1 cut(s) | 398 |
| SsiI | CCGC | 3 cut(s) | 36, 132, 305 |
| SspMI | CTAG | 3 cut(s) | 399, 1155, 1203 |
| StyD4I | CCNGG | 2 cut(s) | 342, 1734 |
| TaaI | ACNGT | 6 cut(s) | 124, 1172, 1368, 1424, 1517, 2024 |
| TaiI | ACGT | 7 cut(s) | 231, 583, 620, 1546, 1918, 1945, 1964 |
| TaqI | TCGA | 2 cut(s) | 633, 1717 |
| TatI | WGTACW | 2 cut(s) | 395, 1051 |
| TauI | GCSGC | 1 cut(s) | 307 |
| TfiI | GAWTC | 7 cut(s) | 470, 1240, 1267, 1317, 1328, 1719, 1772 |
| Tru1I | TTAA | 8 cut(s) | 323, 384, 479, 713, 1122, 1335, 1473, 1964 |
| Tru9I | TTAA | 8 cut(s) | 323, 384, 479, 713, 1122, 1335, 1473, 1964 |
| TscAI | CASTG | 4 cut(s) | 781, 1083, 1610, 1682 |
| TseFI | GTSAC | 3 cut(s) | 93, 241, 268 |
| TseI | GCWGC | 3 cut(s) | 846, 956, 1126 |
| Tsp45I | GTSAC | 3 cut(s) | 93, 241, 268 |
| TspGWI | ACGGA | 2 cut(s) | 80, 463 |
| TspRI | CASTG | 4 cut(s) | 781, 1083, 1610, 1682 |
| Tth111I | GACNNNGTC | 1 cut(s) | 73 |
| VpaK11BI | GGWCC | 1 cut(s) | 82 |
| XapI | RAATTY | 4 cut(s) | 568, 602, 1349, 1648 |
| XmiI | GTMKAC | 1 cut(s) | 1419 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1886 |
| XspI | CTAG | 3 cut(s) | 399, 1155, 1203 |
| Zsp2I | ATGCAT | 1 cut(s) | 6 |