FvH4_5g05120

Belongs to the spermidine spermine synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0019232
Physical Location & Seq
Reverse (-)
2990733 .. 2992965
2233 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g05120.t1

Sequence Viewer

Length: 1083 bp
ATGGCTACAGAGAATGGTGGTACTCTTGCTATGGTTTCTGCAGGTTGTCATGCCGAGGAGGAAATAAAAACTCTAATGGCTAAAGAGACTGGTATTTGTAACAATGGTACTAGTGAGATTTTGGCTTCTCATGAGAATGGAGAGGTAGTCAAGGGAGAGAATGGTAGTAATATATCCGGTACTGCATTTTCTGATGGATGGTATGCTCTTGCTACCACAGATTGGCCTGGGCATGCTGATATGTACAAGATGGAAAAGATTTTGTTCCATGAGAGATCAGAGTTCCAGGACATTTTGGTTTTTCAGTCACAGCAGCATGGAAAGGTAGTCATTTTGGATGGCTATCTTCAACTCACAGAGAAGGATGAGTTTGCTTACCAGGAAATGCTTACTCACCTCGCTCTCTGTTCCATCCCAAACCCAAAGAAGGTATTGCTCATTGGAGGCGGAGACGGTGGGATTTTGCGAGAAGTATCGCGCCATTCTTCTGTTGAACAAATCGACATATGTGAAATTGACAAGATGGTCACAGATGTCTACAAGCGCTTTTTCCCTAACATAGCAGTTGGATATCAGGATCCAAGAGTGAATGTGCACATCGCTGACGGAGTTAAATTCATGCAGTCAGTTCCTGAAGGCACCTATGATGCCATTATACTTGATGCATTTGTGAACATAGGGCCTGGAGACAAAGAGCTTTCTGATCAAAACTTCTTGAAATCAATTGCGCGTGCTCTTCGCCCCGGCGGTGTCATGTCTACACCAGCTGAGAGTTACTGGCATAAGGATTTTGAAGAAATTGCACATAGCATATCGAACTGTTGCAAAGTTTTCAAGGGTTCTGTCAACTATGCTTGGACTACAGTTCCTGCATATTCAAGCGGCATCATTGGTTTCATGCTTTGCTCCACTGAAGGGCCAGCAGTGGACTTCAAACACCCTGTGAATCCACTCAACCCTGACAATTATGGTGTGGCAAAGGTACCCCTCAAGTTCTACAACTCAGAGATGCACACTGCTGCATTTCAGCTCCCCGCTTTCGCAAAAGAGGCCATCGAGGAGGCTATTAGCTCCAAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00330 ko00410 ko00480 ko01100 map00270 map00330 map00410 map00480 map01100
Pfam Domains
Protein Families
Spermine_synt_N Spermine_synth

Protein Analysis

361

Amino Acids

39.5

Weight (kDa)

5.06

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synt_N PF17284 67 - 120 5.6e-16 Spermidine synthase tetramerisation domain
Spermine_synth PF01564 123 - 312 3.1e-59 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019232)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05120 FvH4_5g05120
malus_domestica MD06G1177500.v1.1
pyrus_communis pycom14g15270
rosa_roxburghii Rroxscaffold_3G00261740
rosa_rugosa Rorug07G0012500
rosa_samantha Rh7AG139300 Rh7CG143500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 524
Acc36I ACCTGC 1 cut(s) 32
Acc65I GGTACC 1 cut(s) 982
AccB1I GGYRCC 2 cut(s) 638, 982
AccB7I CCANNNNNTGG 1 cut(s) 222
AccI GTMKAC 2 cut(s) 537, 758
AccII CGCG 2 cut(s) 478, 730
AciI CCGC 4 cut(s) 447, 747, 882, 1035
AclWI GGATC 2 cut(s) 572, 585
AcsI RAATTY 1 cut(s) 614
AcuI CTGAAG 2 cut(s) 654, 933
AdeI CACNNNGTG 1 cut(s) 943
AfaI GTAC 5 cut(s) 22, 109, 181, 245, 984
AfeI AGCGCT 1 cut(s) 545
AfiI CCNNNNNNNGG 3 cut(s) 222, 427, 915
AgsI TTSAA 7 cut(s) 350, 494, 718, 794, 835, 879, 934
AhlI ACTAGT 1 cut(s) 110
AjnI CCWGG 4 cut(s) 226, 285, 378, 682
AluBI AGCT 4 cut(s) 697, 767, 1030, 1071
AluI AGCT 4 cut(s) 697, 767, 1030, 1071
Alw21I GWGCWC 2 cut(s) 597, 736
Alw26I GTCTC 3 cut(s) 80, 444, 681
Alw44I GTGCAC 1 cut(s) 593
AlwI GGATC 2 cut(s) 572, 585
AlwNI CAGNNNCTG 2 cut(s) 632, 869
Aor51HI AGCGCT 1 cut(s) 545
AoxI GGCC 4 cut(s) 224, 680, 917, 1050
ApaLI GTGCAC 1 cut(s) 593
ApeKI GCWGC 2 cut(s) 313, 1019
ApoI RAATTY 1 cut(s) 614
Asp718I GGTACC 1 cut(s) 982
AspLEI GCGC 3 cut(s) 480, 546, 730
AspS9I GGNCC 2 cut(s) 680, 917
AsuC2I CCSGG 1 cut(s) 744
AsuHPI GGTGA 1 cut(s) 386
BaeGI GKGCMC 1 cut(s) 597
BamHI GGATCC 1 cut(s) 577
BanI GGYRCC 2 cut(s) 638, 982
Bbv12I GWGCWC 2 cut(s) 597, 736
BbvI GCAGC 2 cut(s) 325, 1006
BccI CCATC 7 cut(s) 188, 192, 244, 332, 419, 517, 1061
BciT130I CCWGG 4 cut(s) 228, 287, 380, 684
BclI TGATCA 1 cut(s) 703
BcnI CCSGG 1 cut(s) 744
BcoDI GTCTC 3 cut(s) 80, 444, 681
BcuI ACTAGT 1 cut(s) 110
BfaI CTAG 1 cut(s) 111
BfmI CTRYAG 3 cut(s) 6, 39, 861
BfoI RGCGCY 1 cut(s) 547
BfuAI ACCTGC 1 cut(s) 32
BisI GCNGC 3 cut(s) 314, 883, 1020
BlsI GCNGC 3 cut(s) 315, 884, 1021
Bme1390I CCNGG 5 cut(s) 228, 287, 380, 684, 744
BmgT120I GGNCC 2 cut(s) 680, 917
BmiI GGNNCC 3 cut(s) 579, 640, 984
BmrFI CCNGG 5 cut(s) 228, 287, 380, 684, 744
BmsI GCATC 4 cut(s) 637, 652, 894, 999
BpmI CTGGAG 1 cut(s) 705
BpuEI CTTGAG 1 cut(s) 974
BpuMI CCSGG 1 cut(s) 744
BsaBI GATNNNNATC 1 cut(s) 342
BsaJI CCNNGG 3 cut(s) 54, 227, 742
BsaWI WCCGGW 1 cut(s) 176
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 3 cut(s) 222, 427, 915
Bse1I ACTGG 2 cut(s) 94, 782
Bse8I GATNNNNATC 1 cut(s) 342
BseBI CCWGG 4 cut(s) 228, 287, 380, 684
BseDI CCNNGG 3 cut(s) 54, 227, 742
BseGI GGATG 4 cut(s) 203, 343, 370, 411
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 3 cut(s) 222, 427, 915
BseMII CTCAG 2 cut(s) 759, 1017
BseNI ACTGG 2 cut(s) 94, 782
BseRI GAGGAG 2 cut(s) 71, 1073
BseSI GKGCMC 1 cut(s) 597
BseXI GCAGC 2 cut(s) 325, 1006
Bsh1236I CGCG 2 cut(s) 478, 730
BshFI GGCC 4 cut(s) 226, 682, 919, 1052
BshNI GGYRCC 2 cut(s) 638, 982
BsiHKAI GWGCWC 2 cut(s) 597, 736
BsiSI CCGG 2 cut(s) 177, 744
BslI CCNNNNNNNGG 3 cut(s) 222, 427, 915
BsmAI GTCTC 3 cut(s) 80, 444, 681
BsmBI CGTCTC 1 cut(s) 444
BsnI GGCC 4 cut(s) 226, 682, 919, 1052
Bsp1286I GDGCHC 2 cut(s) 597, 736
Bsp1407I TGTACA 1 cut(s) 243
Bsp143I GATC 3 cut(s) 275, 577, 703
BspACI CCGC 4 cut(s) 447, 747, 882, 1035
BspANI GGCC 4 cut(s) 226, 682, 919, 1052
BspCNI CTCAG 2 cut(s) 760, 1016
BspFNI CGCG 2 cut(s) 478, 730
BspHI TCATGA 1 cut(s) 130
BspLI GGNNCC 3 cut(s) 579, 640, 984
BspMAI CTGCAG 1 cut(s) 43
BspMI ACCTGC 1 cut(s) 32
BspPI GGATC 2 cut(s) 572, 585
BspQI GCTCTTC 1 cut(s) 741
BspT107I GGYRCC 2 cut(s) 638, 982
BsrGI TGTACA 1 cut(s) 243
BsrI ACTGG 2 cut(s) 94, 782
BssECI CCNNGG 3 cut(s) 54, 227, 742
BssMI GATC 3 cut(s) 275, 577, 703
Bst2UI CCWGG 4 cut(s) 228, 287, 380, 684
Bst4CI ACNGT 3 cut(s) 455, 821, 865
Bst6I CTCTTC 1 cut(s) 741
BstAUI TGTACA 1 cut(s) 243
BstC8I GCNNGC 3 cut(s) 234, 732, 921
BstDEI CTNAG 2 cut(s) 768, 1003
BstF5I GGATG 4 cut(s) 203, 343, 370, 411
BstFNI CGCG 2 cut(s) 478, 730
BstH2I RGCGCY 1 cut(s) 547
BstHHI GCGC 3 cut(s) 480, 546, 730
BstKTI GATC 3 cut(s) 278, 580, 706
BstMAI GTCTC 3 cut(s) 80, 444, 681
BstMBI GATC 3 cut(s) 275, 577, 703
BstMWI GCNNNNNNNGC 1 cut(s) 1049
BstNI CCWGG 4 cut(s) 228, 287, 380, 684
BstNSI RCATGY 1 cut(s) 236
BstSCI CCNGG 5 cut(s) 226, 285, 378, 682, 742
BstSFI CTRYAG 3 cut(s) 6, 39, 861
BstSLI GKGCMC 1 cut(s) 597
BstUI CGCG 2 cut(s) 478, 730
BstV1I GCAGC 2 cut(s) 325, 1006
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
BsuRI GGCC 4 cut(s) 226, 682, 919, 1052
BtgZI GCGATG 1 cut(s) 583
BtsCI GGATG 4 cut(s) 203, 343, 370, 411
BtsI GCAGTG 2 cut(s) 930, 1014
BtsIMutI CAGTG 3 cut(s) 909, 930, 1014
BveI ACCTGC 1 cut(s) 32
Cac8I GCNNGC 3 cut(s) 234, 732, 921
CaiI CAGNNNCTG 2 cut(s) 632, 869
CciI TCATGA 1 cut(s) 130
CfoI GCGC 3 cut(s) 480, 546, 730
Cfr13I GGNCC 2 cut(s) 680, 917
Csp6I GTAC 5 cut(s) 21, 108, 180, 244, 983
CviAII CATG 8 cut(s) 50, 131, 233, 269, 317, 619, 754, 898
CviQI GTAC 5 cut(s) 21, 108, 180, 244, 983
DdeI CTNAG 2 cut(s) 768, 1003
DpnI GATC 3 cut(s) 277, 579, 705
DpnII GATC 3 cut(s) 275, 577, 703
DraIII CACNNNGTG 1 cut(s) 943
DrdI GACNNNNNNGTC 1 cut(s) 524
DseDI GACNNNNNNGTC 1 cut(s) 524
Eam1104I CTCTTC 1 cut(s) 741
EarI CTCTTC 1 cut(s) 741
EciI GGCGGA 1 cut(s) 462
Eco32I GATATC 1 cut(s) 572
Eco47III AGCGCT 1 cut(s) 545
Eco57I CTGAAG 2 cut(s) 654, 933
EcoO109I RGGNCCY 1 cut(s) 680
EcoRII CCWGG 4 cut(s) 226, 285, 378, 682
EcoRV GATATC 1 cut(s) 572
EcoT22I ATGCAT 1 cut(s) 667
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 8 cut(s) 53, 134, 236, 272, 320, 622, 757, 901
FatI CATG 8 cut(s) 49, 130, 232, 268, 316, 618, 753, 897
FauI CCCGC 1 cut(s) 1042
FauNDI CATATG 1 cut(s) 506
FbaI TGATCA 1 cut(s) 703
FblI GTMKAC 2 cut(s) 537, 758
Fnu4HI GCNGC 3 cut(s) 314, 883, 1020
FokI GGATG 4 cut(s) 210, 350, 377, 398
Fsp4HI GCNGC 3 cut(s) 314, 883, 1020
FspBI CTAG 1 cut(s) 111
GlaI GCGC 3 cut(s) 479, 545, 729
GluI GCNGC 3 cut(s) 314, 883, 1020
GsuI CTGGAG 1 cut(s) 705
HaeII RGCGCY 1 cut(s) 547
HaeIII GGCC 4 cut(s) 226, 682, 919, 1052
HapII CCGG 2 cut(s) 177, 744
HhaI GCGC 3 cut(s) 480, 546, 730
Hin1II CATG 8 cut(s) 53, 134, 236, 272, 320, 622, 757, 901
Hin6I GCGC 3 cut(s) 478, 544, 728
HinP1I GCGC 3 cut(s) 478, 544, 728
HincII GTYRAC 1 cut(s) 847
HindII GTYRAC 1 cut(s) 847
HinfI GANTC 1 cut(s) 946
HpaII CCGG 2 cut(s) 177, 744
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 6 cut(s) 538, 595, 673, 759, 847, 928
Hpy188I TCNGA 5 cut(s) 193, 280, 703, 1006, 1082
Hpy188III TCNNGA 4 cut(s) 131, 575, 632, 715
Hpy8I GTNNAC 6 cut(s) 538, 595, 673, 759, 847, 928
HpyAV CCTTC 4 cut(s) 355, 421, 629, 908
HpyCH4III ACNGT 3 cut(s) 455, 821, 865
HpyF10VI GCNNNNNNNGC 1 cut(s) 1049
HpyF3I CTNAG 2 cut(s) 768, 1003
Hsp92II CATG 8 cut(s) 53, 134, 236, 272, 320, 622, 757, 901
HspAI GCGC 3 cut(s) 478, 544, 728
KpnI GGTACC 1 cut(s) 986
Ksp22I TGATCA 1 cut(s) 703
Kzo9I GATC 3 cut(s) 275, 577, 703
LguI GCTCTTC 1 cut(s) 741
LmnI GCTCC 3 cut(s) 911, 1035, 1076
Lsp1109I GCAGC 2 cut(s) 325, 1006
LweI GCATC 4 cut(s) 637, 652, 894, 999
MaeI CTAG 1 cut(s) 111
MaeIII GTNAC 4 cut(s) 98, 306, 526, 773
MalI GATC 3 cut(s) 277, 579, 705
MboI GATC 3 cut(s) 275, 577, 703
MboII GAAGA 4 cut(s) 338, 477, 728, 806
MfeI CAATTG 1 cut(s) 723
MflI RGATCY 1 cut(s) 577
MhlI GDGCHC 2 cut(s) 597, 736
MluCI AATT 5 cut(s) 513, 614, 723, 798, 964
MmeI TCCRAC 1 cut(s) 547
MnlI CCTC 9 cut(s) 49, 52, 136, 407, 437, 998, 1042, 1051, 1054
Mph1103I ATGCAT 1 cut(s) 667
MseI TTAA 1 cut(s) 612
MslI CAYNNNNRTG 1 cut(s) 135
MspA1I CMGCKG 1 cut(s) 767
MspI CCGG 2 cut(s) 177, 744
MspR9I CCNGG 5 cut(s) 228, 287, 380, 684, 744
MunI CAATTG 1 cut(s) 723
MvaI CCWGG 4 cut(s) 228, 287, 380, 684
MvnI CGCG 2 cut(s) 478, 730
MwoI GCNNNNNNNGC 1 cut(s) 1049
NciI CCSGG 1 cut(s) 744
NdeI CATATG 1 cut(s) 506
NdeII GATC 3 cut(s) 275, 577, 703
NlaIII CATG 8 cut(s) 53, 134, 236, 272, 320, 622, 757, 901
NlaIV GGNNCC 3 cut(s) 579, 640, 984
NmeAIII GCCGAG 1 cut(s) 79
NmuCI GTSAC 2 cut(s) 306, 526
NsiI ATGCAT 1 cut(s) 667
NspI RCATGY 1 cut(s) 236
PaeI GCATGC 1 cut(s) 236
PagI TCATGA 1 cut(s) 130
PciSI GCTCTTC 1 cut(s) 741
PfeI GAWTC 1 cut(s) 946
PflMI CCANNNNNTGG 1 cut(s) 222
PfoI TCCNGGA 1 cut(s) 285
PkrI GCNGC 3 cut(s) 315, 884, 1021
Psp6I CCWGG 4 cut(s) 226, 285, 378, 682
PspGI CCWGG 4 cut(s) 226, 285, 378, 682
PspN4I GGNNCC 3 cut(s) 579, 640, 984
PspPI GGNCC 2 cut(s) 680, 917
PstI CTGCAG 1 cut(s) 43
PstNI CAGNNNCTG 2 cut(s) 632, 869
PsuI RGATCY 1 cut(s) 577
PvuII CAGCTG 1 cut(s) 767
RsaI GTAC 5 cut(s) 22, 109, 181, 245, 984
RsaNI GTAC 5 cut(s) 21, 108, 180, 244, 983
RseI CAYNNNNRTG 1 cut(s) 135
SapI GCTCTTC 1 cut(s) 741
SaqAI TTAA 1 cut(s) 612
SatI GCNGC 3 cut(s) 314, 883, 1020
Sau3AI GATC 3 cut(s) 275, 577, 703
Sau96I GGNCC 2 cut(s) 680, 917
ScrFI CCNGG 5 cut(s) 228, 287, 380, 684, 744
SduI GDGCHC 2 cut(s) 597, 736
SfaNI GCATC 4 cut(s) 637, 652, 894, 999
SfcI CTRYAG 3 cut(s) 6, 39, 861
SmiMI CAYNNNNRTG 1 cut(s) 135
SmlI CTYRAG 1 cut(s) 989
SmoI CTYRAG 1 cut(s) 989
SpeI ACTAGT 1 cut(s) 110
SphI GCATGC 1 cut(s) 236
Sse9I AATT 5 cut(s) 513, 614, 723, 798, 964
SsiI CCGC 4 cut(s) 447, 747, 882, 1035
SspMI CTAG 1 cut(s) 111
StyD4I CCNGG 5 cut(s) 226, 285, 378, 682, 742
TaaI ACNGT 3 cut(s) 455, 821, 865
TaqI TCGA 3 cut(s) 501, 815, 1056
TasI AATT 5 cut(s) 513, 614, 723, 798, 964
TatI WGTACW 1 cut(s) 243
TauI GCSGC 1 cut(s) 885
TfiI GAWTC 1 cut(s) 946
Tru1I TTAA 1 cut(s) 612
Tru9I TTAA 1 cut(s) 612
TscAI CASTG 3 cut(s) 916, 930, 1021
TseFI GTSAC 2 cut(s) 306, 526
TseI GCWGC 2 cut(s) 313, 1019
Tsp45I GTSAC 2 cut(s) 306, 526
TspDTI ATGAA 2 cut(s) 607, 886
TspGWI ACGGA 1 cut(s) 621
TspRI CASTG 3 cut(s) 916, 930, 1021
Van91I CCANNNNNTGG 1 cut(s) 222
VneI GTGCAC 1 cut(s) 593
XapI RAATTY 1 cut(s) 614
XceI RCATGY 1 cut(s) 236
XmiI GTMKAC 2 cut(s) 537, 758
XspI CTAG 1 cut(s) 111
Zsp2I ATGCAT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.