MD06G1177500.v1.1

Belongs to the spermidine spermine synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0019232
Physical Location & Seq
Forward (+)
31725776 .. 31729337
3562 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1177500.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGGCTGAGAAGAACGGTAGTGTTACTGGTTTTGCAGGTTTTGTTGACGAAGACCTTGTGGAGGATGTTGAAATAATGAGTGCAGCTGCAAATGGTCATCAAAATGGTAATACTGAGATGGGATTGGTCTTTGATAATAATGGAAAGGAGAAGGCAGAGGCCATACCTAGTAGTGTTGCATTTACTAAGGCATTTACTAAGGCAGGATGGTATGCTGATGTTTCCACAGATGGACCTGGGAAGGCACATTTATATAAGATGGAGAAAACCTTATTTCATGGGAAGTCAGAGTTTCAAGATCTTTTTGTTTTTCAGTCATCAGAGCATGGCAATGTAGCCATCTTGGATGGTTTTCTTCAACTCACAGAGAGGGATGAATTTGCTTACCAGGAAATGCTCTCTCACCTCCCACTTTGCTCTATCCCAAACCCAAGGAAGGTCTTGCTTGTTGGAGGCGGAGATGGAGGCATTTTGAGGGAAATATCTCGTCATTCTGCTGTTGAAGAAATCGATATATGCGAAATTGACACAATGGTGATAGATGTTTATAAGCGTTTTTTTCCGGACATAGCTGTGGGATATAAGGATCCCAGAGTGCAAGTACGCATAGATGATGGAGTTGCATTCATGAAGTCAGTTCCTCAAGGCACATATGATGCAATTATAATTGATGCATTCCAGGGCATGGGTGCTACAGCACAAGAACTTTCTGATAAGGACTTCCTGGAATCAGTTGCAAGGGCTCTTCGCCCCGGCGGTGTGCTGTCTACCCCGGCGGACAGCTTCTGGAGCAAAGACTTTGTTAACTTTGCCGATACCGTAGCAAACTGCCACAAAATATTCAAGGGCTCCGTCAACTATGCCTGGACAACAGTTCCTGCATATACAAGTGGGATCATTGGGTTCATGCTTTGCTCGAAGGAAGGACCAACCGTGGACTTTAAACACCCTATAAATCCATTAAATCAAGAGAACTATGGCGTGGCAAAGGGACCTCCCAAGTTCTACAACTCAGAGATCCACACTGCTGCGTTTCGACTGCCATCTTTCGCAGAAAAGGTTATCGGTTTCCAGATGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00330 ko00410 ko00480 ko01100 map00270 map00330 map00410 map00480 map01100
Pfam Domains
Protein Families
Spermine_synt_N Spermine_synth

Protein Analysis

361

Amino Acids

39.43

Weight (kDa)

4.98

Isoelectric Point (pI)

30.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synt_N PF17284 69 - 123 3.1e-16 Spermidine synthase tetramerisation domain
Spermine_synth PF01564 126 - 315 1.6e-58 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019232)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05120 FvH4_5g05120
malus_domestica MD06G1177500.v1.1
pyrus_communis pycom14g15270
rosa_roxburghii Rroxscaffold_3G00261740
rosa_rugosa Rorug07G0012500
rosa_samantha Rh7AG139300 Rh7CG143500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 549, 665
Acc36I ACCTGC 1 cut(s) 26
AccB7I CCANNNNNTGG 1 cut(s) 685
AccI GTMKAC 1 cut(s) 767
AccIII TCCGGA 1 cut(s) 562
AciI CCGC 3 cut(s) 456, 756, 776
AclWI GGATC 4 cut(s) 581, 594, 902, 1012
AcsI RAATTY 1 cut(s) 377
AfaI GTAC 1 cut(s) 603
AfiI CCNNNNNNNGG 3 cut(s) 61, 436, 685
AgsI TTSAA 5 cut(s) 71, 296, 359, 503, 844
AjnI CCWGG 5 cut(s) 235, 387, 678, 723, 863
AleI CACNNNNGTG 1 cut(s) 533
AluBI AGCT 3 cut(s) 86, 572, 783
AluI AGCT 3 cut(s) 86, 572, 783
AlwI GGATC 4 cut(s) 581, 594, 902, 1012
AlwNI CAGNNNCTG 2 cut(s) 786, 878
Aor13HI TCCGGA 1 cut(s) 562
AoxI GGCC 1 cut(s) 159
ApeKI GCWGC 3 cut(s) 83, 86, 1028
ApoI RAATTY 1 cut(s) 377
AspS9I GGNCC 3 cut(s) 233, 926, 992
AsuC2I CCSGG 2 cut(s) 753, 773
AsuHPI GGTGA 2 cut(s) 395, 547
AvaII GGWCC 3 cut(s) 233, 926, 992
BamHI GGATCC 1 cut(s) 586
BanII GRGCYC 2 cut(s) 745, 851
BbsI GAAGAC 1 cut(s) 57
BbvI GCAGC 3 cut(s) 73, 95, 1015
BccI CCATC 9 cut(s) 112, 201, 224, 253, 341, 347, 455, 608, 1051
BciT130I CCWGG 5 cut(s) 237, 389, 680, 725, 865
BcnI CCSGG 2 cut(s) 753, 773
BfaI CTAG 1 cut(s) 168
BfmI CTRYAG 1 cut(s) 693
BfuAI ACCTGC 1 cut(s) 26
BglII AGATCT 1 cut(s) 298
BisI GCNGC 3 cut(s) 84, 87, 1029
BlsI GCNGC 3 cut(s) 85, 88, 1030
Bme1390I CCNGG 7 cut(s) 237, 389, 680, 725, 753, 773, 865
Bme18I GGWCC 3 cut(s) 233, 926, 992
BmgT120I GGNCC 3 cut(s) 233, 926, 992
BmiI GGNNCC 3 cut(s) 588, 850, 993
BmrFI CCNGG 7 cut(s) 237, 389, 680, 725, 753, 773, 865
BmsI GCATC 2 cut(s) 646, 661
BpiI GAAGAC 1 cut(s) 57
BpmI CTGGAG 1 cut(s) 808
BpuEI CTTGAG 1 cut(s) 627
BpuMI CCSGG 2 cut(s) 753, 773
Bsa29I ATCGAT 1 cut(s) 510
BsaJI CCNNGG 6 cut(s) 236, 431, 679, 751, 771, 933
BsaWI WCCGGW 1 cut(s) 562
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 436, 685
Bse1I ACTGG 1 cut(s) 31
Bse3DI GCAATG 1 cut(s) 337
BseAI TCCGGA 1 cut(s) 562
BseBI CCWGG 5 cut(s) 237, 389, 680, 725, 865
BseCI ATCGAT 1 cut(s) 510
BseDI CCNNGG 6 cut(s) 236, 431, 679, 751, 771, 933
BseGI GGATG 4 cut(s) 70, 212, 352, 379
BseLI CCNNNNNNNGG 3 cut(s) 61, 436, 685
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 2 cut(s) 105, 1026
BseNI ACTGG 1 cut(s) 31
BseXI GCAGC 3 cut(s) 73, 95, 1015
BsgI GTGCAG 1 cut(s) 102
BshFI GGCC 1 cut(s) 161
BshVI ATCGAT 1 cut(s) 510
BsiSI CCGG 3 cut(s) 563, 753, 773
BslFI GGGAC 1 cut(s) 1005
BslI CCNNNNNNNGG 3 cut(s) 61, 436, 685
BsmFI GGGAC 1 cut(s) 1005
BsmI GAATGC 2 cut(s) 623, 674
BsnI GGCC 1 cut(s) 161
Bsp1286I GDGCHC 2 cut(s) 745, 851
Bsp13I TCCGGA 1 cut(s) 562
Bsp143I GATC 4 cut(s) 298, 586, 894, 1017
BspACI CCGC 3 cut(s) 456, 756, 776
BspANI GGCC 1 cut(s) 161
BspCNI CTCAG 2 cut(s) 106, 1025
BspDI ATCGAT 1 cut(s) 510
BspEI TCCGGA 1 cut(s) 562
BspHI TCATGA 2 cut(s) 627, 1079
BspLI GGNNCC 3 cut(s) 588, 850, 993
BspMI ACCTGC 1 cut(s) 26
BspPI GGATC 4 cut(s) 581, 594, 902, 1012
BspQI GCTCTTC 1 cut(s) 750
BsrDI GCAATG 1 cut(s) 337
BsrI ACTGG 1 cut(s) 31
BssECI CCNNGG 6 cut(s) 236, 431, 679, 751, 771, 933
BssMI GATC 4 cut(s) 298, 586, 894, 1017
BssT1I CCWWGG 1 cut(s) 431
Bst2UI CCWGG 5 cut(s) 237, 389, 680, 725, 865
Bst4CI ACNGT 4 cut(s) 17, 820, 874, 934
Bst6I CTCTTC 1 cut(s) 750
BstDEI CTNAG 5 cut(s) 6, 114, 186, 198, 1012
BstDSI CCRYGG 1 cut(s) 933
BstENI CCTNNNNNAGG 1 cut(s) 59
BstF5I GGATG 4 cut(s) 70, 212, 352, 379
BstKTI GATC 4 cut(s) 301, 589, 897, 1020
BstMBI GATC 4 cut(s) 298, 586, 894, 1017
BstMWI GCNNNNNNNGC 1 cut(s) 789
BstNI CCWGG 5 cut(s) 237, 389, 680, 725, 865
BstSCI CCNGG 7 cut(s) 235, 387, 678, 723, 751, 771, 863
BstSFI CTRYAG 1 cut(s) 693
BstV1I GCAGC 3 cut(s) 73, 95, 1015
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 3 cut(s) 298, 586, 1017
BstYI RGATCY 3 cut(s) 298, 586, 1017
Bsu15I ATCGAT 1 cut(s) 510
BsuRI GGCC 1 cut(s) 161
BsuTUI ATCGAT 1 cut(s) 510
BtgI CCRYGG 1 cut(s) 933
BtsCI GGATG 4 cut(s) 70, 212, 352, 379
BtsI GCAGTG 1 cut(s) 1023
BtsIMutI CAGTG 1 cut(s) 1023
BveI ACCTGC 1 cut(s) 26
CaiI CAGNNNCTG 2 cut(s) 786, 878
CciI TCATGA 2 cut(s) 627, 1079
Cfr13I GGNCC 3 cut(s) 233, 926, 992
ClaI ATCGAT 1 cut(s) 510
Csp6I GTAC 1 cut(s) 602
CviAII CATG 6 cut(s) 278, 326, 628, 685, 907, 1080
CviJI RGCY 8 cut(s) 5, 86, 161, 338, 572, 743, 783, 849
CviKI_1 RGCY 8 cut(s) 5, 86, 161, 338, 572, 743, 783, 849
CviQI GTAC 1 cut(s) 602
DdeI CTNAG 5 cut(s) 6, 114, 186, 198, 1012
DpnI GATC 4 cut(s) 300, 588, 896, 1019
DpnII GATC 4 cut(s) 298, 586, 894, 1017
DraI TTTAAA 1 cut(s) 943
Eam1104I CTCTTC 1 cut(s) 750
EarI CTCTTC 1 cut(s) 750
EciI GGCGGA 2 cut(s) 471, 791
Eco130I CCWWGG 1 cut(s) 431
Eco24I GRGCYC 2 cut(s) 745, 851
Eco47I GGWCC 3 cut(s) 233, 926, 992
EcoNI CCTNNNNNAGG 1 cut(s) 59
EcoO109I RGGNCCY 1 cut(s) 992
EcoRII CCWGG 5 cut(s) 235, 387, 678, 723, 863
EcoT14I CCWWGG 1 cut(s) 431
EcoT22I ATGCAT 1 cut(s) 676
EcoT38I GRGCYC 2 cut(s) 745, 851
ErhI CCWWGG 1 cut(s) 431
FaeI CATG 6 cut(s) 281, 329, 631, 688, 910, 1083
FaqI GGGAC 1 cut(s) 1005
FatI CATG 6 cut(s) 277, 325, 627, 684, 906, 1079
FauNDI CATATG 1 cut(s) 652
FblI GTMKAC 1 cut(s) 767
Fnu4HI GCNGC 3 cut(s) 84, 87, 1029
FokI GGATG 4 cut(s) 77, 219, 359, 386
FriOI GRGCYC 2 cut(s) 745, 851
Fsp4HI GCNGC 3 cut(s) 84, 87, 1029
FspBI CTAG 1 cut(s) 168
GluI GCNGC 3 cut(s) 84, 87, 1029
GsuI CTGGAG 1 cut(s) 808
HaeIII GGCC 1 cut(s) 161
HapII CCGG 3 cut(s) 563, 753, 773
Hin1II CATG 6 cut(s) 281, 329, 631, 688, 910, 1083
HincII GTYRAC 3 cut(s) 46, 805, 856
HindII GTYRAC 3 cut(s) 46, 805, 856
HinfI GANTC 1 cut(s) 728
HpaI GTTAAC 1 cut(s) 805
HpaII CCGG 3 cut(s) 563, 753, 773
HphI GGTGA 2 cut(s) 395, 547
Hpy166II GTNNAC 5 cut(s) 46, 768, 805, 856, 937
Hpy188I TCNGA 4 cut(s) 289, 322, 712, 1015
Hpy188III TCNNGA 7 cut(s) 296, 563, 628, 787, 968, 1072, 1080
Hpy8I GTNNAC 5 cut(s) 46, 768, 805, 856, 937
HpyAV CCTTC 5 cut(s) 145, 235, 430, 913, 917
HpyCH4III ACNGT 4 cut(s) 17, 820, 874, 934
HpyF10VI GCNNNNNNNGC 1 cut(s) 789
HpyF3I CTNAG 5 cut(s) 6, 114, 186, 198, 1012
Hsp92II CATG 6 cut(s) 281, 329, 631, 688, 910, 1083
Kpn2I TCCGGA 1 cut(s) 562
KspAI GTTAAC 1 cut(s) 805
Kzo9I GATC 4 cut(s) 298, 586, 894, 1017
LguI GCTCTTC 1 cut(s) 750
LmnI GCTCC 2 cut(s) 789, 854
Lsp1109I GCAGC 3 cut(s) 73, 95, 1015
LweI GCATC 2 cut(s) 646, 661
MaeI CTAG 1 cut(s) 168
MaeIII GTNAC 1 cut(s) 22
MalI GATC 4 cut(s) 300, 588, 896, 1019
MboI GATC 4 cut(s) 298, 586, 894, 1017
MboII GAAGA 5 cut(s) 22, 62, 347, 515, 737
MflI RGATCY 3 cut(s) 298, 586, 1017
MhlI GDGCHC 2 cut(s) 745, 851
MluCI AATT 4 cut(s) 377, 522, 660, 666
MmeI TCCRAC 1 cut(s) 430
MnlI CCTC 9 cut(s) 55, 151, 363, 416, 446, 458, 468, 651, 1005
Mph1103I ATGCAT 1 cut(s) 676
MroI TCCGGA 1 cut(s) 562
MseI TTAA 3 cut(s) 804, 942, 962
MslI CAYNNNNRTG 4 cut(s) 102, 330, 533, 572
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 3 cut(s) 563, 753, 773
MspR9I CCNGG 7 cut(s) 237, 389, 680, 725, 753, 773, 865
Mva1269I GAATGC 2 cut(s) 623, 674
MvaI CCWGG 5 cut(s) 237, 389, 680, 725, 865
MwoI GCNNNNNNNGC 1 cut(s) 789
NciI CCSGG 2 cut(s) 753, 773
NdeI CATATG 1 cut(s) 652
NdeII GATC 4 cut(s) 298, 586, 894, 1017
NlaIII CATG 6 cut(s) 281, 329, 631, 688, 910, 1083
NlaIV GGNNCC 3 cut(s) 588, 850, 993
NsiI ATGCAT 1 cut(s) 676
OliI CACNNNNGTG 1 cut(s) 533
PagI TCATGA 2 cut(s) 627, 1079
PciSI GCTCTTC 1 cut(s) 750
PcsI WCGNNNNNNNCGW 1 cut(s) 516
PctI GAATGC 2 cut(s) 623, 674
PfeI GAWTC 1 cut(s) 728
PflMI CCANNNNNTGG 1 cut(s) 685
PfoI TCCNGGA 1 cut(s) 723
PkrI GCNGC 3 cut(s) 85, 88, 1030
PpuMI RGGWCCY 1 cut(s) 992
PsiI TTATAA 2 cut(s) 549, 665
Psp5II RGGWCCY 1 cut(s) 992
Psp6I CCWGG 5 cut(s) 235, 387, 678, 723, 863
PspGI CCWGG 5 cut(s) 235, 387, 678, 723, 863
PspN4I GGNNCC 3 cut(s) 588, 850, 993
PspPI GGNCC 3 cut(s) 233, 926, 992
PspPPI RGGWCCY 1 cut(s) 992
PstNI CAGNNNCTG 2 cut(s) 786, 878
PsuI RGATCY 3 cut(s) 298, 586, 1017
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 1 cut(s) 603
RsaNI GTAC 1 cut(s) 602
RseI CAYNNNNRTG 4 cut(s) 102, 330, 533, 572
SapI GCTCTTC 1 cut(s) 750
SaqAI TTAA 3 cut(s) 804, 942, 962
SatI GCNGC 3 cut(s) 84, 87, 1029
Sau3AI GATC 4 cut(s) 298, 586, 894, 1017
Sau96I GGNCC 3 cut(s) 233, 926, 992
ScrFI CCNGG 7 cut(s) 237, 389, 680, 725, 753, 773, 865
SduI GDGCHC 2 cut(s) 745, 851
SfaNI GCATC 2 cut(s) 646, 661
SfcI CTRYAG 1 cut(s) 693
SinI GGWCC 3 cut(s) 233, 926, 992
SmiMI CAYNNNNRTG 4 cut(s) 102, 330, 533, 572
SmlI CTYRAG 1 cut(s) 642
SmoI CTYRAG 1 cut(s) 642
Sse9I AATT 4 cut(s) 377, 522, 660, 666
SsiI CCGC 3 cut(s) 456, 756, 776
SspI AATATT 1 cut(s) 840
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 7 cut(s) 235, 387, 678, 723, 751, 771, 863
StyI CCWWGG 1 cut(s) 431
TaaI ACNGT 4 cut(s) 17, 820, 874, 934
TaqI TCGA 3 cut(s) 510, 917, 1036
TasI AATT 4 cut(s) 377, 522, 660, 666
TfiI GAWTC 1 cut(s) 728
Tru1I TTAA 3 cut(s) 804, 942, 962
Tru9I TTAA 3 cut(s) 804, 942, 962
TscAI CASTG 1 cut(s) 1030
TseI GCWGC 3 cut(s) 83, 86, 1028
TspDTI ATGAA 5 cut(s) 266, 390, 616, 644, 895
TspGWI ACGGA 1 cut(s) 841
TspRI CASTG 1 cut(s) 1030
Van91I CCANNNNNTGG 1 cut(s) 685
VpaK11BI GGWCC 3 cut(s) 233, 926, 992
XagI CCTNNNNNAGG 1 cut(s) 59
XapI RAATTY 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 767
XspI CTAG 1 cut(s) 168
Zsp2I ATGCAT 1 cut(s) 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.