Rroxscaffold_3G00261740

Belongs to the spermidine spermine synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0019232
Physical Location & Seq
Reverse (-)
55905093 .. 55908618
3526 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00261740.1

Sequence Viewer

Length: 585 bp
ATGAAGGCCGGATCGATAGATCGGGCTAGCGGCGGTGGCCGTTTCGACGAAGCGCGAGGCCGTGAGTTGTTGGGTCAAGCATCCGATGTCGGGGAGAGGGATAGGTCGTGGCGGCGTGGTCCGGACGGTGTCAAGGGGCTTCTCGTACGACTGCGGTGCGAGGTCCGAAACACCGAGGTCTCTCCCTCGTCCTCATTGTCTCGCTTCTCCTCTCAACCATGGTTTTCCCACTTCCAATTGCAATCGACCATGGTCTCCGTTGGGCCTGGAGCCAAAGACCTTTCTGACCAACACTTCTTGAAATCAACTGCACGTGATCTTCGCCCTGGCCGTGTCATGTCTACACCAGCTGAGAATTACTGGCATAAGGATTTTGAAGAAATTGCAGATAGCATATCGAACTGTCGCAAAGTTTTCAAGGGGCCAGCAGTGGACTTCAAACACCCTGTGAATCCACTGAACCCTGAGAATTATGGTGTGGCAAAGGTACCCCACAAGTTCTACAACTCGGAGATGCACATTGCTGCATTCCAGCTCCCGGCATTCGCAAAGAAGGCCATCGAGGCTATTAGCTCCAAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00330 ko00410 ko00480 ko01100 map00270 map00330 map00410 map00480 map01100
Pfam Domains
Protein Families
Spermine_synt_N Spermine_synth

Protein Analysis

194

Amino Acids

21.58

Weight (kDa)

9.57

Isoelectric Point (pI)

49.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019232)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05120 FvH4_5g05120
malus_domestica MD06G1177500.v1.1
pyrus_communis pycom14g15270
rosa_roxburghii Rroxscaffold_3G00261740
rosa_rugosa Rorug07G0012500
rosa_samantha Rh7AG139300 Rh7CG143500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 487
AccB1I GGYRCC 1 cut(s) 487
AccI GTMKAC 1 cut(s) 341
AccII CGCG 1 cut(s) 55
AccIII TCCGGA 1 cut(s) 121
AciI CCGC 4 cut(s) 30, 33, 112, 154
AclWI GGATC 1 cut(s) 19
AcoI YGGCCR 2 cut(s) 37, 328
AcvI CACGTG 1 cut(s) 314
AdeI CACNNNGTG 1 cut(s) 448
AfaI GTAC 2 cut(s) 147, 489
AfiI CCNNNNNNNGG 2 cut(s) 90, 538
AgsI TTSAA 4 cut(s) 301, 377, 418, 439
AjnI CCWGG 2 cut(s) 265, 325
AluBI AGCT 3 cut(s) 350, 535, 573
AluI AGCT 3 cut(s) 350, 535, 573
Alw26I GTCTC 3 cut(s) 184, 204, 259
AlwI GGATC 1 cut(s) 19
Aor13HI TCCGGA 1 cut(s) 121
AoxI GGCC 7 cut(s) 6, 37, 58, 263, 328, 422, 555
ApeKI GCWGC 1 cut(s) 524
Asp718I GGTACC 1 cut(s) 487
AspLEI GCGC 1 cut(s) 55
AspS9I GGNCC 4 cut(s) 119, 163, 263, 422
AsuC2I CCSGG 1 cut(s) 539
AsuNHI GCTAGC 1 cut(s) 26
AvaII GGWCC 2 cut(s) 119, 163
BanI GGYRCC 1 cut(s) 487
BbrPI CACGTG 1 cut(s) 314
BbvI GCAGC 1 cut(s) 511
BccI CCATC 1 cut(s) 566
BceAI ACGGC 3 cut(s) 24, 45, 315
BcgI CGANNNNNNTGC 2 cut(s) 138, 172
BciT130I CCWGG 2 cut(s) 267, 327
BcnI CCSGG 1 cut(s) 539
BcoDI GTCTC 3 cut(s) 184, 204, 259
BfaI CTAG 1 cut(s) 27
BglI GCCNNNNNGGC 1 cut(s) 563
BisI GCNGC 3 cut(s) 31, 113, 525
BlsI GCNGC 3 cut(s) 32, 114, 526
Bme1390I CCNGG 3 cut(s) 267, 327, 539
Bme18I GGWCC 2 cut(s) 119, 163
BmgT120I GGNCC 4 cut(s) 119, 163, 263, 422
BmiI GGNNCC 3 cut(s) 271, 423, 489
BmrFI CCNGG 3 cut(s) 267, 327, 539
BmsI GCATC 2 cut(s) 89, 504
BmtI GCTAGC 1 cut(s) 30
BoxI GACNNNNGTC 1 cut(s) 251
BpmI CTGGAG 1 cut(s) 288
BpuMI CCSGG 1 cut(s) 539
Bsa29I ATCGAT 1 cut(s) 14
BsaAI YACGTR 1 cut(s) 314
BsaI GGTCTC 2 cut(s) 184, 259
BsaJI CCNNGG 4 cut(s) 174, 218, 249, 325
BsaWI WCCGGW 1 cut(s) 121
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 538
Bse1I ACTGG 1 cut(s) 365
Bse3DI GCAATG 1 cut(s) 519
BseAI TCCGGA 1 cut(s) 121
BseBI CCWGG 2 cut(s) 267, 327
BseCI ATCGAT 1 cut(s) 14
BseDI CCNNGG 4 cut(s) 174, 218, 249, 325
BseGI GGATG 1 cut(s) 80
BseLI CCNNNNNNNGG 2 cut(s) 90, 538
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 2 cut(s) 342, 456
BseNI ACTGG 1 cut(s) 365
BseRI GAGGAG 1 cut(s) 199
BseXI GCAGC 1 cut(s) 511
BsgI GTGCAG 1 cut(s) 294
Bsh1236I CGCG 1 cut(s) 55
BshFI GGCC 7 cut(s) 8, 39, 60, 265, 330, 424, 557
BshNI GGYRCC 1 cut(s) 487
BshVI ATCGAT 1 cut(s) 14
BsiSI CCGG 3 cut(s) 9, 122, 539
BsiWI CGTACG 1 cut(s) 145
BslI CCNNNNNNNGG 2 cut(s) 90, 538
BsmAI GTCTC 3 cut(s) 184, 204, 259
BsmI GAATGC 2 cut(s) 527, 542
BsnI GGCC 7 cut(s) 8, 39, 60, 265, 330, 424, 557
Bso31I GGTCTC 2 cut(s) 184, 259
Bsp13I TCCGGA 1 cut(s) 121
Bsp143I GATC 3 cut(s) 11, 19, 316
Bsp19I CCATGG 2 cut(s) 218, 249
BspACI CCGC 4 cut(s) 30, 33, 112, 154
BspANI GGCC 7 cut(s) 8, 39, 60, 265, 330, 424, 557
BspCNI CTCAG 2 cut(s) 343, 457
BspDI ATCGAT 1 cut(s) 14
BspEI TCCGGA 1 cut(s) 121
BspFNI CGCG 1 cut(s) 55
BspLI GGNNCC 3 cut(s) 271, 423, 489
BspOI GCTAGC 1 cut(s) 30
BspPI GGATC 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 487
BspTNI GGTCTC 2 cut(s) 184, 259
BsrDI GCAATG 1 cut(s) 519
BsrI ACTGG 1 cut(s) 365
BssECI CCNNGG 4 cut(s) 174, 218, 249, 325
BssMI GATC 3 cut(s) 11, 19, 316
BssT1I CCWWGG 2 cut(s) 218, 249
Bst2UI CCWGG 2 cut(s) 267, 327
Bst4CI ACNGT 2 cut(s) 128, 404
BstBAI YACGTR 1 cut(s) 314
BstC8I GCNNGC 2 cut(s) 28, 426
BstDEI CTNAG 2 cut(s) 351, 465
BstDSI CCRYGG 2 cut(s) 218, 249
BstF5I GGATG 1 cut(s) 80
BstFNI CGCG 1 cut(s) 55
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 3 cut(s) 14, 22, 319
BstMAI GTCTC 3 cut(s) 184, 204, 259
BstMBI GATC 3 cut(s) 11, 19, 316
BstMWI GCNNNNNNNGC 3 cut(s) 36, 554, 563
BstNI CCWGG 2 cut(s) 267, 327
BstPAI GACNNNNGTC 1 cut(s) 251
BstSCI CCNGG 3 cut(s) 265, 325, 537
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 1 cut(s) 511
Bsu15I ATCGAT 1 cut(s) 14
BsuRI GGCC 7 cut(s) 8, 39, 60, 265, 330, 424, 557
BsuTUI ATCGAT 1 cut(s) 14
BtgI CCRYGG 2 cut(s) 218, 249
BtsCI GGATG 1 cut(s) 80
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 2 cut(s) 435, 455
Cac8I GCNNGC 2 cut(s) 28, 426
CfoI GCGC 1 cut(s) 55
Cfr13I GGNCC 4 cut(s) 119, 163, 263, 422
ClaI ATCGAT 1 cut(s) 14
Csp6I GTAC 2 cut(s) 146, 488
CviAII CATG 3 cut(s) 219, 250, 337
CviQI GTAC 2 cut(s) 146, 488
DdeI CTNAG 2 cut(s) 351, 465
DpnI GATC 3 cut(s) 13, 21, 318
DpnII GATC 3 cut(s) 11, 19, 316
DraIII CACNNNGTG 1 cut(s) 448
EaeI YGGCCR 2 cut(s) 37, 328
Eco130I CCWWGG 2 cut(s) 218, 249
Eco31I GGTCTC 2 cut(s) 184, 259
Eco47I GGWCC 2 cut(s) 119, 163
Eco72I CACGTG 1 cut(s) 314
EcoRII CCWGG 2 cut(s) 265, 325
EcoT14I CCWWGG 2 cut(s) 218, 249
ErhI CCWWGG 2 cut(s) 218, 249
FaeI CATG 3 cut(s) 222, 253, 340
FaiI YATR 6 cut(s) 220, 251, 338, 366, 395, 474
FatI CATG 3 cut(s) 218, 249, 336
FblI GTMKAC 1 cut(s) 341
Fnu4HI GCNGC 3 cut(s) 31, 113, 525
FokI GGATG 1 cut(s) 67
Fsp4HI GCNGC 3 cut(s) 31, 113, 525
FspBI CTAG 1 cut(s) 27
GlaI GCGC 1 cut(s) 54
GluI GCNGC 3 cut(s) 31, 113, 525
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 7 cut(s) 8, 39, 60, 265, 330, 424, 557
HapII CCGG 3 cut(s) 9, 122, 539
HhaI GCGC 1 cut(s) 55
Hin1II CATG 3 cut(s) 222, 253, 340
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HinfI GANTC 1 cut(s) 451
HpaII CCGG 3 cut(s) 9, 122, 539
Hpy166II GTNNAC 2 cut(s) 342, 433
Hpy188I TCNGA 5 cut(s) 85, 167, 286, 511, 584
Hpy188III TCNNGA 2 cut(s) 122, 298
Hpy8I GTNNAC 2 cut(s) 342, 433
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 1 cut(s) 547
HpyCH4III ACNGT 2 cut(s) 128, 404
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 5 cut(s) 241, 311, 386, 517, 527
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 554, 563
HpyF3I CTNAG 2 cut(s) 351, 465
HpySE526I ACGT 1 cut(s) 313
Hsp92II CATG 3 cut(s) 222, 253, 340
HspAI GCGC 1 cut(s) 53
Kpn2I TCCGGA 1 cut(s) 121
KpnI GGTACC 1 cut(s) 491
Kzo9I GATC 3 cut(s) 11, 19, 316
LmnI GCTCC 3 cut(s) 269, 540, 578
Lsp1109I GCAGC 1 cut(s) 511
LweI GCATC 2 cut(s) 89, 504
MaeI CTAG 1 cut(s) 27
MaeII ACGT 1 cut(s) 313
MalI GATC 3 cut(s) 13, 21, 318
MboI GATC 3 cut(s) 11, 19, 316
MboII GAAGA 2 cut(s) 311, 389
MfeI CAATTG 1 cut(s) 236
MluCI AATT 4 cut(s) 236, 355, 381, 469
MnlI CCTC 8 cut(s) 50, 90, 154, 169, 196, 202, 220, 556
MroI TCCGGA 1 cut(s) 121
MspA1I CMGCKG 1 cut(s) 350
MspI CCGG 3 cut(s) 9, 122, 539
MspR9I CCNGG 3 cut(s) 267, 327, 539
MunI CAATTG 1 cut(s) 236
Mva1269I GAATGC 2 cut(s) 527, 542
MvaI CCWGG 2 cut(s) 267, 327
MvnI CGCG 1 cut(s) 55
MwoI GCNNNNNNNGC 3 cut(s) 36, 554, 563
NciI CCSGG 1 cut(s) 539
NcoI CCATGG 2 cut(s) 218, 249
NdeII GATC 3 cut(s) 11, 19, 316
NheI GCTAGC 1 cut(s) 26
NlaIII CATG 3 cut(s) 222, 253, 340
NlaIV GGNNCC 3 cut(s) 271, 423, 489
PcsI WCGNNNNNNNCGW 1 cut(s) 328
PctI GAATGC 2 cut(s) 527, 542
PfeI GAWTC 1 cut(s) 451
Pfl23II CGTACG 1 cut(s) 145
PflFI GACNNNGTC 1 cut(s) 128
PkrI GCNGC 3 cut(s) 32, 114, 526
PmaCI CACGTG 1 cut(s) 314
PmlI CACGTG 1 cut(s) 314
Ppu21I YACGTR 1 cut(s) 314
PshAI GACNNNNGTC 1 cut(s) 251
Psp6I CCWGG 2 cut(s) 265, 325
PspCI CACGTG 1 cut(s) 314
PspGI CCWGG 2 cut(s) 265, 325
PspLI CGTACG 1 cut(s) 145
PspN4I GGNNCC 3 cut(s) 271, 423, 489
PspPI GGNCC 4 cut(s) 119, 163, 263, 422
PsyI GACNNNGTC 1 cut(s) 128
PvuII CAGCTG 1 cut(s) 350
RsaI GTAC 2 cut(s) 147, 489
RsaNI GTAC 2 cut(s) 146, 488
SatI GCNGC 3 cut(s) 31, 113, 525
Sau3AI GATC 3 cut(s) 11, 19, 316
Sau96I GGNCC 4 cut(s) 119, 163, 263, 422
ScrFI CCNGG 3 cut(s) 267, 327, 539
SetI ASST 9 cut(s) 107, 165, 180, 282, 316, 352, 489, 537, 575
SfaNI GCATC 2 cut(s) 89, 504
SinI GGWCC 2 cut(s) 119, 163
Sse9I AATT 4 cut(s) 236, 355, 381, 469
SsiI CCGC 4 cut(s) 30, 33, 112, 154
SspMI CTAG 1 cut(s) 27
StyD4I CCNGG 3 cut(s) 265, 325, 537
StyI CCWWGG 2 cut(s) 218, 249
TaaI ACNGT 2 cut(s) 128, 404
TaiI ACGT 1 cut(s) 316
TaqI TCGA 5 cut(s) 14, 45, 245, 398, 561
TasI AATT 4 cut(s) 236, 355, 381, 469
TauI GCSGC 2 cut(s) 33, 115
TfiI GAWTC 1 cut(s) 451
TscAI CASTG 2 cut(s) 435, 462
TseI GCWGC 1 cut(s) 524
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 247
TspRI CASTG 2 cut(s) 435, 462
Tth111I GACNNNGTC 1 cut(s) 128
VpaK11BI GGWCC 2 cut(s) 119, 163
XmiI GTMKAC 1 cut(s) 341
XspI CTAG 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.