FvH4_5g05550

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012246
Physical Location & Seq
Forward (+)
3260315 .. 3266940
6626 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g05550.t3

Sequence Viewer

Length: 3246 bp
ATGGGTTTCCTTAGAGTTTCATGGGCTGTTTTGCTTCTTTGGGTTTACTCAGTCTTGTGCTTGAAAGCACAGTCTTCTAGTTCAGAGGTCTCTCTTAAGTTCTTGAAAGCTCCTCATGCATTTTCTCATCTGAACTCAGCCACATTTGCATTTGAAGCTTTGGTTGGTGGCAATGCTAGCGCATGCACCGATTGCAGTTTCAGTTGCAAGCTAGATGATGGCAATGGTTCAAACTGTGGAACTGGGAAAGCTTTATACTCAGGATTGCAGGATGGAAAGCATACATTTGAGGTCTGCACCAATGGGACTCAAGGTGTTGGTTGTGCCTACTATAACTGGACTGTTGATACAGTACCCCCAACAGCATCTGTTGCTGCCTCTAGATCTTTTACAACTGCTACCAATGTTTCAGTAAATATTTCTTTCAGTGAATCCTGCAACGGTGGAGGAGGTTTTGGATGTTCGTCTGTAAACACCTGCAACCTGCTAGTCTATGGTGCTGGCCAAGTTATACCATCTTCACTCAATATTCTGGAGCCAAACCTCAAATATTCTCTTGTTGTGGGTTTATCATCTTCTGTTCAATATGGGCGAGTGATCCTGGTAATGGATAAGAATTTCTGCTCTGATGTTGCTGGAAACAGATTCGTAAGAACCGAAAATTCGAGTTTCTATGTACGTATTGATAGAAGACCTGTCTTTTGCAACCTGAGGATTCATATTCCTGAAACACTACTTCAGCTAAATGGTGAAACTCGAACAGTGCAGGCCACACATAATCATAATAACCTAAAGATTTATCTATATTTCTCAGAACCTGTCCTGAATACATCTGCACAAATTTTGAAGTCTATCCACATAAGTCAGGGCATACTTTTACCAAATAGCACAAACAACTCTGGGAATCGCAGATTTGGCTTTGCGGTTTCAAACATACCTAGCATTGCTATAATCACAGTGAGCGTCAATTCAAATTTGATAATAACCAGACAAGGGACTCCAGTTTCTCCAATTTCACCTGCAACTTTCCTTTTCGATTCTCAAAGGCCTGCGGTGAAGTTGAGTACAACCTCAAGCATGAGAACAAGAGAACACAGTATTTCCATATTAATAAAGTTCATGAAGCCAGTATTTGGTTTCAACTCCTCTTCTTTAGTACTCTCAGGAGGACATTTACAGAGCTTTCATGAAATAAGTAGGAGTATGTACACTGTCAATATAGAAGCAATTAATGATATTGTTTCGGTTAATGTTCCTGAAAACGTATCTGGGGATGTTGCTGGAAACAAAAATCTAGCTTCAAATGTTCTTCAAGTATCACACTATACAGTGCCCATGATATCTTCTCTGCTATCTGCATTTGTGACTGCTCTTTTTGCCCTGACATCCATAACTGCGGGGCTGCTTTATATTTCAACTGCAAGCCTTCAATCCCTATGGACATTTAGGAGAGCATCTTCTTTGGCATCTCATCCAGCAAGAAATCTTTTTAGAATTGCTTGTCATATACAAATCTTTGCACTCTCTAGATGGTTGGCAGTTACATTGCCAATTGATTATTATGAATTTTCAAGGGGTTTACAATGGAGTATTCCTTACTTCAATCTCCCATGGGAATCTGGGAAGAGCTTCCAAGCCTCAAGTCCTTACACCAGCTCAAAGTCCTACACTAATGAAGGTTATGATTCAGAAATTTTCAAAAGCAAGCAGCTAGAAGAAGAGAGTTCTGATAAGGTTACTTCAGTATATGGGTTGCCACTTACTCCAATGGAATACAGAACGTTCTTTGAGTTGGAGGGTGAAAATTTTAAACCTGAAGCCGAGTATCTCTCCGGTTCTTATAACAGGTGGAGGAATTTTGATAGAACCATGTTCTGGTTAGCGGTGATTAGTGGTAGCTTGCTCCTAGTACATATTTTACTCCTTTTCATTCTGAAACTGAGGAAGAAAAATTCTGAGAGACAGAGTGGTTATGGAGCACTCACATTCCCAAGATTTGAGATATTTCTTGTAATTCTTGCACTACCTGGTATTTGTGAGGCCTCCGCTGCACTAGTTAAAGGAGGAGCGTCATCTGGAGTTACAGTTGGCTGTCTGTTGTTGGCTATCACGTCTTTTCTGCTGCTAGTCTTGTTCCTTTTCCTCTCAATCGGCATTACATTTGGGAAGTTACTTCAATACAAGGAAGTACATCAAGAAGGCCAGAAATTTCTCTGGTTTCAAGAGATTGTCCGAGTGACACTGGGTCCAGGAAAAAGAGGTCAGTGGACATGGAAAGAAAAACCAAGCTCTGTGTATCTAATTATCTTTGGCCCTCTCTTTGAAGATCTGAGAGGCCCTCCAAAATACATGCTCTCTCAGATTTCTGAGGGCAGTGCTCGCAAGCAAGGTGAGCGTATTATTGACTCTGATGATGAAACAGAAGATGCAGAAGCACCTTTTATCCAGAAACTGTTTGGAATACTCAGAATATACTACACACTATTAGAATGTTTGAAAAGGGTTTCTGTTGGGATCATGGCTGGTGTCTACATGGACAGATGGAATTCAAGAACTCCTTCAATTACCTTACTGTGCATAACCTCTTTTCAACTTTTCTTCCTCGTTCTTAAGAAGCCGTTTATCAAGAAAAAGGTTCAGTTGGTTGAGATCGTCTCGGTTGCCAGTGAAGTAGGTTTATTTGCAACTTGTTTGGTTCTCTTGGAGAAGGAATTTTCAGCCAGTGACAGGACTAAGCTTGGTATTTTCATCATACTGCTGTTCTTAGTCGGATACATTGCACAGATGATTAATGAATGGTATGCATTGTATAGACAAACATTGCTTCTGGACCCTGCCGAGAACTCCTTCTTTACTGGTTTGAAATTAGCTGCAACCGGCTGTGTCTTGTTATTCATCCCGCGGAGATTCATCAATAACTTGGAAAGCAAGTTCCAAGAAAGGCAACAGGTGGTGGGAGCAACAAGGGATACTAGCTCTTCTGAGTACCGGAGATCCGGGAGTAGGGGCACCACTCCAACTGATAGACCATGGCTAAAGCAGCTGAGAGAGTTGGCAAAGTCTAGCTTTAGTAAAGAAGGAAGTAGCGGATTTGCAAATGACCCTTCGAGCAGTAACACTCGAACTAGATGGAGCGGGTTCTGGAGCAGCAAAATGAGTGGAAGCTCATCTAAGTCTTCTGGTGATTCGAAATCAAAACCAAGAGGGTTGTACAAAGACTTGGAGGCTATTTTTGCATCCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1082

Amino Acids

119.68

Weight (kDa)

9.21

Isoelectric Point (pI)

45.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Big_12 PF19078 144 - 235 6.4e-06 Bacterial Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012246)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48200
fragaria_vesca FvH4_5g05550 FvH4_5g05550 FvH4_5g05550
malus_domestica MD06G1171200.v1.1
prunus_persica Prupe.5G170900_v2.0.a1 Prupe.5G170900_v2.0.a1 Prupe.5G170900_v2.0.a1 Prupe.5G170900_v2.0.a1
pyrus_communis pycom06g15260
rosa_chinensis RchiOBHm_Chr7g0193221
rosa_laevigata RLG00000004285
rosa_roxburghii Rroxscaffold_3G00262270
rosa_rugosa Rorug07G0008400
rosa_samantha Rh7AG133900 Rh7BG134900 Rh7CG137900 Rh7DG135900
rosa_wichuraiana Rw7G011450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1842
AarI CACCTGC 2 cut(s) 485, 1027
Acc36I ACCTGC 3 cut(s) 485, 492, 1027
AccB1I GGYRCC 1 cut(s) 3008
AccB7I CCANNNNNTGG 2 cut(s) 1133, 1875
AccBSI CCGCTC 1 cut(s) 3135
AccI GTMKAC 1 cut(s) 2529
AccII CGCG 1 cut(s) 2902
AciI CCGC 9 cut(s) 923, 1052, 1397, 1883, 2046, 2900, 2902, 3087, 3135
AclI AACGTT 1 cut(s) 1781
AclWI GGATC 3 cut(s) 592, 2522, 2988
AcoI YGGCCR 1 cut(s) 502
AcuI CTGAAG 3 cut(s) 722, 1725, 1836
AfaI GTAC 9 cut(s) 354, 678, 1066, 1158, 1208, 1911, 2190, 2987, 3212
AfiI CCNNNNNNNGG 6 cut(s) 607, 993, 1133, 1875, 2727, 3003
AflII CTTAAG 2 cut(s) 95, 2609
AhdI GACNNNNNGTC 1 cut(s) 2244
AhlI ACTAGT 1 cut(s) 2053
AjiI CACGTC 1 cut(s) 2112
AjnI CCWGG 3 cut(s) 600, 2026, 2248
AjuI GAANNNNNNNTTGG 2 cut(s) 147, 179
AloI GAACNNNNNNTCC 2 cut(s) 2915, 2947
Alw21I GWGCWC 2 cut(s) 1981, 2380
Alw26I GTCTC 3 cut(s) 94, 1954, 2659
AlwI GGATC 3 cut(s) 592, 2522, 2988
AlwNI CAGNNNCTG 3 cut(s) 368, 818, 2452
AoxI GGCC 7 cut(s) 502, 768, 1046, 2040, 2200, 2311, 2335
ApeKI GCWGC 8 cut(s) 374, 1402, 1708, 2048, 2122, 2870, 3040, 3147
ArsI GACNNNNNNTTYG 2 cut(s) 684, 716
AseI ATTAAT 3 cut(s) 1109, 1230, 2790
Asp700I GAANNNNTTC 3 cut(s) 1628, 2557, 2846
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 4 cut(s) 2246, 2312, 2336, 2830
AsuC2I CCSGG 1 cut(s) 2998
AsuHPI GGTGA 7 cut(s) 761, 1008, 1066, 1811, 1897, 2402, 3194
AsuII TTCGAA 1 cut(s) 3188
AsuNHI GCTAGC 1 cut(s) 176
AvaII GGWCC 2 cut(s) 2246, 2830
AxyI CCTNAGG 1 cut(s) 710
BaeGI GKGCMC 2 cut(s) 1335, 3011
BalI TGGCCA 1 cut(s) 504
BanI GGYRCC 1 cut(s) 3008
BbsI GAAGAC 3 cut(s) 66, 697, 3168
Bbv12I GWGCWC 2 cut(s) 1981, 2380
BbvI GCAGC 8 cut(s) 361, 1389, 1720, 2035, 2109, 2857, 3052, 3159
BccI CCATC 6 cut(s) 212, 266, 523, 1524, 2535, 3123
BceAI ACGGC 1 cut(s) 2602
BciT130I CCWGG 3 cut(s) 602, 2028, 2250
BciVI GTATCC 2 cut(s) 2765, 2962
BcnI CCSGG 1 cut(s) 2998
BcoDI GTCTC 3 cut(s) 94, 1954, 2659
BcuI ACTAGT 1 cut(s) 2053
BfrI CTTAAG 2 cut(s) 95, 2609
BfuAI ACCTGC 3 cut(s) 485, 492, 1027
BfuI GTATCC 2 cut(s) 2765, 2962
BglII AGATCT 2 cut(s) 383, 2326
BisI GCNGC 8 cut(s) 375, 1403, 1709, 2049, 2123, 2871, 3041, 3148
BlsI GCNGC 8 cut(s) 376, 1404, 1710, 2050, 2124, 2872, 3042, 3149
BmcAI AGTACT 1 cut(s) 1158
Bme1390I CCNGG 4 cut(s) 602, 2028, 2250, 2998
Bme18I GGWCC 2 cut(s) 2246, 2830
BmeRI GACNNNNNGTC 1 cut(s) 2244
BmgBI CACGTC 1 cut(s) 2112
BmgT120I GGNCC 4 cut(s) 2246, 2312, 2336, 2830
BmiI GGNNCC 4 cut(s) 537, 2247, 2832, 3010
BmrFI CCNGG 4 cut(s) 602, 2028, 2250, 2998
BmrI ACTGGG 2 cut(s) 252, 2252
BmsI GCATC 4 cut(s) 374, 1463, 1475, 2416
BmtI GCTAGC 1 cut(s) 180
BmuI ACTGGG 2 cut(s) 252, 2252
BpiI GAAGAC 3 cut(s) 66, 697, 3168
BplI GAGNNNNNCTC 6 cut(s) 1814, 1846, 2323, 2355, 2639, 2671
BpmI CTGGAG 4 cut(s) 554, 984, 2097, 3163
Bpu14I TTCGAA 1 cut(s) 3188
BpuEI CTTGAG 3 cut(s) 294, 1057, 1624
BpuMI CCSGG 1 cut(s) 2998
BsaAI YACGTR 1 cut(s) 680
BsaI GGTCTC 1 cut(s) 94
BsaJI CCNNGG 3 cut(s) 1610, 2900, 3029
BsaWI WCCGGW 2 cut(s) 1832, 2988
BsaXI ACNNNNNCTCC 2 cut(s) 2070, 2100
Bsc4I CCNNNNNNNGG 6 cut(s) 607, 993, 1133, 1875, 2727, 3003
Bse118I RCCGGY 1 cut(s) 2876
Bse1I ACTGG 8 cut(s) 247, 341, 1001, 1127, 2247, 2664, 2721, 2860
Bse21I CCTNAGG 1 cut(s) 710
Bse3DI GCAATG 6 cut(s) 178, 229, 942, 1544, 2775, 2819
BseBI CCWGG 3 cut(s) 602, 2028, 2250
BseDI CCNNGG 3 cut(s) 1610, 2900, 3029
BseGI GGATG 7 cut(s) 277, 464, 1279, 1385, 1471, 2895, 3236
BseLI CCNNNNNNNGG 6 cut(s) 607, 993, 1133, 1875, 2727, 3003
BseMI GCAATG 6 cut(s) 178, 229, 942, 1544, 2775, 2819
BseNI ACTGG 8 cut(s) 247, 341, 1001, 1127, 2247, 2664, 2721, 2860
BseRI GAGGAG 4 cut(s) 102, 462, 1135, 2079
BseSI GKGCMC 2 cut(s) 1335, 3011
BseXI GCAGC 8 cut(s) 361, 1389, 1720, 2035, 2109, 2857, 3052, 3159
BsgI GTGCAG 4 cut(s) 280, 785, 819, 2034
Bsh1236I CGCG 1 cut(s) 2902
BshFI GGCC 7 cut(s) 504, 770, 1048, 2042, 2202, 2313, 2337
BshNI GGYRCC 1 cut(s) 3008
BsiHKAI GWGCWC 2 cut(s) 1981, 2380
BsiSI CCGG 4 cut(s) 1833, 2877, 2989, 2997
BslFI GGGAC 2 cut(s) 319, 1009
BslI CCNNNNNNNGG 6 cut(s) 607, 993, 1133, 1875, 2727, 3003
BsmAI GTCTC 3 cut(s) 94, 1954, 2659
BsmBI CGTCTC 1 cut(s) 2659
BsmFI GGGAC 2 cut(s) 319, 1009
BsnI GGCC 7 cut(s) 504, 770, 1048, 2042, 2202, 2313, 2337
Bso31I GGTCTC 1 cut(s) 94
Bsp119I TTCGAA 1 cut(s) 3188
Bsp1286I GDGCHC 4 cut(s) 1335, 1981, 2380, 3011
Bsp1407I TGTACA 2 cut(s) 1206, 3210
Bsp143I GATC 6 cut(s) 383, 597, 2326, 2514, 2649, 2993
Bsp19I CCATGG 2 cut(s) 1610, 3029
BspACI CCGC 9 cut(s) 923, 1052, 1397, 1883, 2046, 2900, 2902, 3087, 3135
BspANI GGCC 7 cut(s) 504, 770, 1048, 2042, 2202, 2313, 2337
BspFNI CGCG 1 cut(s) 2902
BspHI TCATGA 2 cut(s) 1119, 1186
BspLI GGNNCC 4 cut(s) 537, 2247, 2832, 3010
BspMI ACCTGC 3 cut(s) 485, 492, 1027
BspOI GCTAGC 1 cut(s) 180
BspPI GGATC 3 cut(s) 592, 2522, 2988
BspQI GCTCTTC 2 cut(s) 1619, 2983
BspT104I TTCGAA 1 cut(s) 3188
BspT107I GGYRCC 1 cut(s) 3008
BspTI CTTAAG 2 cut(s) 95, 2609
BspTNI GGTCTC 1 cut(s) 94
BsrBI CCGCTC 1 cut(s) 3135
BsrDI GCAATG 6 cut(s) 178, 229, 942, 1544, 2775, 2819
BsrFI RCCGGY 1 cut(s) 2876
BsrGI TGTACA 2 cut(s) 1206, 3210
BsrI ACTGG 8 cut(s) 247, 341, 1001, 1127, 2247, 2664, 2721, 2860
BssAI RCCGGY 1 cut(s) 2876
BssECI CCNNGG 3 cut(s) 1610, 2900, 3029
BssMI GATC 6 cut(s) 383, 597, 2326, 2514, 2649, 2993
BssT1I CCWWGG 2 cut(s) 1610, 3029
Bst2UI CCWGG 3 cut(s) 602, 2028, 2250
Bst6I CTCTTC 4 cut(s) 1153, 1619, 1713, 2983
BstAFI CTTAAG 2 cut(s) 95, 2609
BstAPI GCANNNNNTGC 2 cut(s) 192, 371
BstAUI TGTACA 2 cut(s) 1206, 3210
BstBAI YACGTR 1 cut(s) 680
BstBI TTCGAA 1 cut(s) 3188
BstDSI CCRYGG 3 cut(s) 1610, 2900, 3029
BstF5I GGATG 7 cut(s) 277, 464, 1279, 1385, 1471, 2895, 3236
BstFNI CGCG 1 cut(s) 2902
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 6 cut(s) 386, 600, 2329, 2517, 2652, 2996
BstMAI GTCTC 3 cut(s) 94, 1954, 2659
BstMBI GATC 6 cut(s) 383, 597, 2326, 2514, 2649, 2993
BstNI CCWGG 3 cut(s) 602, 2028, 2250
BstNSI RCATGY 2 cut(s) 186, 2353
BstSCI CCNGG 4 cut(s) 600, 2026, 2248, 2996
BstSLI GKGCMC 2 cut(s) 1335, 3011
BstSNI TACGTA 1 cut(s) 680
BstUI CGCG 1 cut(s) 2902
BstV1I GCAGC 8 cut(s) 361, 1389, 1720, 2035, 2109, 2857, 3052, 3159
BstV2I GAAGAC 3 cut(s) 66, 697, 3168
BstX2I RGATCY 3 cut(s) 383, 2326, 2993
BstYI RGATCY 3 cut(s) 383, 2326, 2993
Bsu36I CCTNAGG 1 cut(s) 710
BsuI GTATCC 2 cut(s) 2765, 2962
BsuRI GGCC 7 cut(s) 504, 770, 1048, 2042, 2202, 2313, 2337
BtgI CCRYGG 3 cut(s) 1610, 2900, 3029
BtrI CACGTC 1 cut(s) 2112
BtsCI GGATG 7 cut(s) 277, 464, 1279, 1385, 1471, 2895, 3236
BtsI GCAGTG 1 cut(s) 2380
BveI ACCTGC 3 cut(s) 485, 492, 1027
CaiI CAGNNNCTG 3 cut(s) 368, 818, 2452
CciI TCATGA 2 cut(s) 1119, 1186
CfoI GCGC 1 cut(s) 182
Cfr10I RCCGGY 1 cut(s) 2876
Cfr13I GGNCC 4 cut(s) 2246, 2312, 2336, 2830
Cfr42I CCGCGG 1 cut(s) 2903
CseI GACGC 2 cut(s) 952, 2058
CsiI ACCWGGT 1 cut(s) 2026
Csp6I GTAC 9 cut(s) 353, 677, 1065, 1157, 1207, 1910, 2189, 2986, 3211
CspCI CAANNNNNGTGG 2 cut(s) 3139, 3174
CviQI GTAC 9 cut(s) 353, 677, 1065, 1157, 1207, 1910, 2189, 2986, 3211
DpnI GATC 6 cut(s) 385, 599, 2328, 2516, 2651, 2995
DpnII GATC 6 cut(s) 383, 597, 2326, 2514, 2649, 2993
DraI TTTAAA 1 cut(s) 1810
DriI GACNNNNNGTC 1 cut(s) 2244
EaeI YGGCCR 1 cut(s) 502
Eam1104I CTCTTC 4 cut(s) 1153, 1619, 1713, 2983
Eam1105I GACNNNNNGTC 1 cut(s) 2244
EarI CTCTTC 4 cut(s) 1153, 1619, 1713, 2983
Eco105I TACGTA 1 cut(s) 680
Eco130I CCWWGG 2 cut(s) 1610, 3029
Eco147I AGGCCT 2 cut(s) 1048, 2042
Eco31I GGTCTC 1 cut(s) 94
Eco32I GATATC 1 cut(s) 1341
Eco47I GGWCC 2 cut(s) 2246, 2830
Eco57I CTGAAG 3 cut(s) 722, 1725, 1836
Eco81I CCTNAGG 1 cut(s) 710
EcoO109I RGGNCCY 1 cut(s) 2336
EcoRI GAATTC 1 cut(s) 2545
EcoRII CCWGG 3 cut(s) 600, 2026, 2248
EcoRV GATATC 1 cut(s) 1341
EcoT14I CCWWGG 2 cut(s) 1610, 3029
EcoT22I ATGCAT 2 cut(s) 121, 2806
ErhI CCWWGG 2 cut(s) 1610, 3029
Esp3I CGTCTC 1 cut(s) 2659
FalI AAGNNNNNCTT 4 cut(s) 2542, 2574, 3050, 3082
FaqI GGGAC 2 cut(s) 319, 1009
FauI CCCGC 3 cut(s) 1390, 2907, 3128
FblI GTMKAC 1 cut(s) 2529
Fnu4HI GCNGC 8 cut(s) 375, 1403, 1709, 2049, 2123, 2871, 3041, 3148
FokI GGATG 7 cut(s) 284, 471, 1286, 1372, 1458, 2882, 3223
Fsp4HI GCNGC 8 cut(s) 375, 1403, 1709, 2049, 2123, 2871, 3041, 3148
GlaI GCGC 1 cut(s) 181
GluI GCNGC 8 cut(s) 375, 1403, 1709, 2049, 2123, 2871, 3041, 3148
GsuI CTGGAG 4 cut(s) 554, 984, 2097, 3163
HaeIII GGCC 7 cut(s) 504, 770, 1048, 2042, 2202, 2313, 2337
HapII CCGG 4 cut(s) 1833, 2877, 2989, 2997
HgaI GACGC 2 cut(s) 952, 2058
HhaI GCGC 1 cut(s) 182
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HindIII AAGCTT 3 cut(s) 156, 249, 2735
HpaII CCGG 4 cut(s) 1833, 2877, 2989, 2997
HphI GGTGA 7 cut(s) 761, 1008, 1066, 1811, 1897, 2402, 3194
Hpy166II GTNNAC 6 cut(s) 46, 472, 1209, 1580, 2268, 2530
Hpy8I GTNNAC 6 cut(s) 46, 472, 1209, 1580, 2268, 2530
HpyAV CCTTC 8 cut(s) 1436, 1670, 2192, 2568, 2701, 2857, 3071, 3114
HpyCH4IV ACGT 4 cut(s) 679, 1263, 1781, 2111
HpySE526I ACGT 4 cut(s) 679, 1263, 1781, 2111
HspAI GCGC 1 cut(s) 180
KspI CCGCGG 1 cut(s) 2903
Kzo9I GATC 6 cut(s) 383, 597, 2326, 2514, 2649, 2993
LguI GCTCTTC 2 cut(s) 1619, 2983
LmnI GCTCC 8 cut(s) 115, 535, 1908, 1976, 2066, 2957, 3132, 3144
Lsp1109I GCAGC 8 cut(s) 361, 1389, 1720, 2035, 2109, 2857, 3052, 3159
LweI GCATC 4 cut(s) 374, 1463, 1475, 2416
MabI ACCWGGT 1 cut(s) 2026
MaeII ACGT 4 cut(s) 679, 1263, 1781, 2111
MaeIII GTNAC 8 cut(s) 1363, 1540, 1735, 2080, 2169, 2236, 2723, 3113
MalI GATC 6 cut(s) 385, 599, 2328, 2516, 2651, 2995
MbiI CCGCTC 1 cut(s) 3135
MboI GATC 6 cut(s) 383, 597, 2326, 2514, 2649, 2993
MfeI CAATTG 1 cut(s) 1551
MflI RGATCY 3 cut(s) 383, 2326, 2993
MhlI GDGCHC 4 cut(s) 1335, 1981, 2380, 3011
MlsI TGGCCA 1 cut(s) 504
MluNI TGGCCA 1 cut(s) 504
MlyI GAGTC 3 cut(s) 301, 991, 2399
MmeI TCCRAC 3 cut(s) 1773, 2749, 3041
Mox20I TGGCCA 1 cut(s) 504
Mph1103I ATGCAT 2 cut(s) 121, 2806
MroXI GAANNNNTTC 3 cut(s) 1628, 2557, 2846
MscI TGGCCA 1 cut(s) 504
MseI TTAA 8 cut(s) 96, 1109, 1230, 1248, 1809, 2058, 2610, 2790
MslI CAYNNNNRTG 1 cut(s) 3241
Msp20I TGGCCA 1 cut(s) 504
MspA1I CMGCKG 3 cut(s) 2048, 2902, 3043
MspCI CTTAAG 2 cut(s) 95, 2609
MspI CCGG 4 cut(s) 1833, 2877, 2989, 2997
MspR9I CCNGG 4 cut(s) 602, 2028, 2250, 2998
MunI CAATTG 1 cut(s) 1551
MvaI CCWGG 3 cut(s) 602, 2028, 2250
MvnI CGCG 1 cut(s) 2902
NciI CCSGG 1 cut(s) 2998
NcoI CCATGG 2 cut(s) 1610, 3029
NdeII GATC 6 cut(s) 383, 597, 2326, 2514, 2649, 2993
NheI GCTAGC 1 cut(s) 176
NlaIV GGNNCC 4 cut(s) 537, 2247, 2832, 3010
NmeAIII GCCGAG 2 cut(s) 1846, 2863
NmuCI GTSAC 3 cut(s) 1363, 2236, 2723
NsiI ATGCAT 2 cut(s) 121, 2806
NspI RCATGY 2 cut(s) 186, 2353
NspV TTCGAA 1 cut(s) 3188
PaeI GCATGC 1 cut(s) 186
PagI TCATGA 2 cut(s) 1119, 1186
PaqCI CACCTGC 2 cut(s) 485, 1027
PceI AGGCCT 2 cut(s) 1048, 2042
PciSI GCTCTTC 2 cut(s) 1619, 2983
PcsI WCGNNNNNNNCGW 1 cut(s) 654
PdmI GAANNNNTTC 3 cut(s) 1628, 2557, 2846
PfeI GAWTC 9 cut(s) 431, 645, 715, 904, 1037, 1616, 1685, 2907, 3185
PflMI CCANNNNNTGG 2 cut(s) 1133, 1875
PfoI TCCNGGA 2 cut(s) 2248, 2996
PkrI GCNGC 8 cut(s) 376, 1404, 1710, 2050, 2124, 2872, 3042, 3149
PleI GAGTC 3 cut(s) 301, 991, 2399
PpsI GAGTC 3 cut(s) 301, 991, 2399
Ppu21I YACGTR 1 cut(s) 680
PshBI ATTAAT 3 cut(s) 1109, 1230, 2790
PsiI TTATAA 1 cut(s) 1842
Psp1406I AACGTT 1 cut(s) 1781
Psp6I CCWGG 3 cut(s) 600, 2026, 2248
PspGI CCWGG 3 cut(s) 600, 2026, 2248
PspN4I GGNNCC 4 cut(s) 537, 2247, 2832, 3010
PspPI GGNCC 4 cut(s) 2246, 2312, 2336, 2830
PstNI CAGNNNCTG 3 cut(s) 368, 818, 2452
PsuI RGATCY 3 cut(s) 383, 2326, 2993
PvuII CAGCTG 1 cut(s) 3043
RsaI GTAC 9 cut(s) 354, 678, 1066, 1158, 1208, 1911, 2190, 2987, 3212
RsaNI GTAC 9 cut(s) 353, 677, 1065, 1157, 1207, 1910, 2189, 2986, 3211
RseI CAYNNNNRTG 1 cut(s) 3241
SacII CCGCGG 1 cut(s) 2903
SapI GCTCTTC 2 cut(s) 1619, 2983
SaqAI TTAA 8 cut(s) 96, 1109, 1230, 1248, 1809, 2058, 2610, 2790
SatI GCNGC 8 cut(s) 375, 1403, 1709, 2049, 2123, 2871, 3041, 3148
Sau3AI GATC 6 cut(s) 383, 597, 2326, 2514, 2649, 2993
Sau96I GGNCC 4 cut(s) 2246, 2312, 2336, 2830
ScaI AGTACT 1 cut(s) 1158
SchI GAGTC 3 cut(s) 301, 991, 2399
ScrFI CCNGG 4 cut(s) 602, 2028, 2250, 2998
SduI GDGCHC 4 cut(s) 1335, 1981, 2380, 3011
SexAI ACCWGGT 1 cut(s) 2026
SfaNI GCATC 4 cut(s) 374, 1463, 1475, 2416
Sfr303I CCGCGG 1 cut(s) 2903
SfuI TTCGAA 1 cut(s) 3188
SgrBI CCGCGG 1 cut(s) 2903
SinI GGWCC 2 cut(s) 2246, 2830
SmiMI CAYNNNNRTG 1 cut(s) 3241
SmlI CTYRAG 5 cut(s) 95, 309, 1072, 1639, 2609
SmoI CTYRAG 5 cut(s) 95, 309, 1072, 1639, 2609
SnaBI TACGTA 1 cut(s) 680
SpeI ACTAGT 1 cut(s) 2053
SphI GCATGC 1 cut(s) 186
SseBI AGGCCT 2 cut(s) 1048, 2042
SsiI CCGC 9 cut(s) 923, 1052, 1397, 1883, 2046, 2900, 2902, 3087, 3135
SspI AATATT 3 cut(s) 418, 529, 551
StuI AGGCCT 2 cut(s) 1048, 2042
StyD4I CCNGG 4 cut(s) 600, 2026, 2248, 2996
StyI CCWWGG 2 cut(s) 1610, 3029
TaiI ACGT 4 cut(s) 682, 1266, 1784, 2114
TaqI TCGA 6 cut(s) 665, 757, 1035, 3107, 3121, 3188
TatI WGTACW 6 cut(s) 1064, 1156, 1206, 1909, 2188, 3210
TfiI GAWTC 9 cut(s) 431, 645, 715, 904, 1037, 1616, 1685, 2907, 3185
Tru1I TTAA 8 cut(s) 96, 1109, 1230, 1248, 1809, 2058, 2610, 2790
Tru9I TTAA 8 cut(s) 96, 1109, 1230, 1248, 1809, 2058, 2610, 2790
TseFI GTSAC 3 cut(s) 1363, 2236, 2723
TseI GCWGC 8 cut(s) 374, 1402, 1708, 2048, 2122, 2870, 3040, 3147
Tsp45I GTSAC 3 cut(s) 1363, 2236, 2723
Van91I CCANNNNNTGG 2 cut(s) 1133, 1875
Vha464I CTTAAG 2 cut(s) 95, 2609
VpaK11BI GGWCC 2 cut(s) 2246, 2830
VspI ATTAAT 3 cut(s) 1109, 1230, 2790
XbaI TCTAGA 2 cut(s) 380, 1526
XceI RCATGY 2 cut(s) 186, 2353
XcmI CCANNNNNNNNNTGG 1 cut(s) 2453
XmiI GTMKAC 1 cut(s) 2529
XmnI GAANNNNTTC 3 cut(s) 1628, 2557, 2846
ZrmI AGTACT 1 cut(s) 1158
Zsp2I ATGCAT 2 cut(s) 121, 2806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.