RchiOBHm_Chr7g0193221

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0012246
Physical Location & Seq
Reverse (-)
11623274 .. 11629282
6009 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17277

Sequence Viewer

Length: 3249 bp
ATGGCTTTGCTTAGAGTTTCATGGGCTGTTTTGTTTCTTTGGGTTTTCTCAGTTTTGTGCTTAGAGGCACAGTCTGATAGTTCAGAGGTCTCTCTTAAGTTCTTGAAAGCTCCTCATGCATTTTCTCATCTGAACTCAGCCACATTTGCATTTGAAGCTTTGGTTGGTGGGAATGCTAGCACATGCACCAATTGCAGTTTCAGTTGCAAGCTAGATGATGGCATTGGTTCGAATTGTGGAACTGGGAAAGTTTTGTACTCAGGATTGCAGGATGGAAAGCATACATTTGATGTCTGCACCAATGGGACTCAAGGAGCTGGTTGTGCCTACTATAACTGGACTGTTGATACAGTACCCCCAACAGCATCTGTTACTGCCTCAAGATCTTTTACAAATGCTACGAGTGTATCAGTAAATATTTCTTTCAGTGAATCCTGCAACGGCGGAGGAGGTTTTGGATGCTCATCTGTGAACACCTGCAACCTGCTAGTCTATGGTGCTGGCGAAGTTATACCATCTTCACTTAATATTCTGGAGCCAAACCTCAAATATTCTCTTGATGTGGGTTTATCATCTTCTGTTCACTATGGACGAGTGATCCTGGTAATGGATAAGAACTTCTGCTCTGATGTTGCTGGAAACAGATTCGTAAGAACCAAAAATTCACGTTTCTATGTACGCATTGATAGAAGACCTGTCTTTTCCAACCTGAGGATTCATATTCCTGAAACACTGCTTCAGTTAAATGGTGAAACTCGAACAGTGCAGGCGACTAATAATCATAATAACTTAAAGATGTATCTATATTTCTCAGAACCTGTCCTGAATACATCTGCACAAATTTTAAACACTATCAACATAAGTCAGGGCTTACTTTTACCGGATAGCACAAAGAACTCTGGAAATCGCAGATTTGGCTTTGCGGTTTCAAACACATCTAGCATTGCTATAATCACGGTGAGCCTCGATTCAAATTTGATAATAACCAGACAAGGGACTCCAGTTTCTCCAATTTCACCAGCAACTTTCCTTTTCGATTCTCAAAGGCCTGCAGTGAAGTTGAGTACAACCTCAAGCATGAGAACAAGAGAAGACACTATTTCTATATTAATAAAATTCATGAAGCCTGTATTTGGTTTCAACTCTTCTTCTTTAGCACTCTCAGGAGGGCATTTACAAAGCTTTTGTGAAGTAAGTAGGAGTATGTACACTGTCAATATAGAAGCAATTAATGATATTGTTTCGGTCAATGTTCCTGAAAATGTAACTGGGGATGTTGCTGGAAACAAAAATCTAGCATCAAATGTTCTTCAAGTATCACACTATTCAGTGCCCATGATATCTTCTCTGGTATTTGCATTTGTGACTGCTCTTTTTGCATTGACATCCATAACTGCGGGGCTGCTTTCTATTTCAACTGCAAGCCTTCAATCCCTTTGGACATTTAGGAGAGCATCTTCTTTGGCATCTCATCCAGCAAGAAATCTTTTCAGAATTGCTTGTCATATTCAAATCTTTGCACTCTCTAGATGGTTGGCAGTTACATTGCCAGTTGATTATTATGAATTTGCAAGGGGTTTACAATGGAGTATTCCTCACTTTAATCTCCCATGGGAAGCTGGGCAAAGCTTCCAAGCCTCAATTCCTTCCACCAGCTCAAAGTCCTACAGGACTGAAGGCTATGATTCAGAAATTTTCAAAAGCATGCAACTAGAAGAAGAGAGTTCTAATAAGGTTACTTCAGTATATGGGTTGCCACTTACTCCAATGGAATACAGAATGTTTTTTGAGTTGGAGGGTGAAAATATTAAACCTGAAGCCGAATATCTCTCCAGTTCTTATAACTCGAACAGGTGGAGGAATTTCGATCGAAGCATGTTCTGGTTAGCGGTGATTAGTGGTAGTTTGATCCTAGTACATGTTTTACTCCTTTTCATTCTGAAACTGAGGAAGAAAAGTTCTGAGAGACAGAGCGGTTATGGAGCACTCACATTCCCAAGATTTGAGATATTTCTTGTAATTCTTGCATTACCTTGTATTTTTGAGGCCTCAGCTGCACTAGTTAGAGGAGCGTCATCTGGAGTTATAGTTGGCAGTCTCTTGTTGGCTGTCACGTCTTTTCTGCTGCTAGTCTTGTTCCTTTTCCTCTCAATTGGCATTACATTTGGAAAGTTACTTCAATACAAGGAAGTACATCAAGAAGGCCAGAAATTTCTCTGGTTTCAAGATATTGTCCGAGTGACACTGGGTCCGGGAAAAAGAGGTCAGTGGACATGGAAAAAAAAACCAAGCTCTGTATATCTAATCATTTTTGGCCCTCTCTTTGAAGATCTCAGAGGCCCCCCAAAGTACATGCTCTCTCAGATTTCTGTGGGCAATGCTCGCAAGCAAGGTGAGCGTATTATTGACTCTGATGATGAAACAGAAGATGCAGAAGCACCTTTTATCCAGAAGGTGTTTGGAATACTCAGAATATACTACACACTATTAGAATGTTTGAAAAGGGTTTTTGTTGGGATCATGGCTGGTGTCTATATGGACAGATGGAACTCTAAAACTCCTTCAATTACCTTATTGTGCATAACCTCTTTTCAACTTTTCTTCCTCGTTCTTAAGAAGCCGTTTATCAAGAAAAAGGTTCAGTTGGTTGAGATCATCTCGGTTTCCAGTGAAGTAGGTTTATTTGCAACTTGCTTGGTTCTCTTGGAGAAGGATTTTTCAGCCAGTGACAGCACTAAAGTTGGTATTTTCATGATAATACTGTTCTTAGTTGGATACATTGCACAGATGATTAATGAGTGGTATGCATTGTATAGACAAACATTGCTTCTGGACCCAGCCGAGAACTCCTTCTTAACTGGTTTTAAATTAGCTGTAACTGGCTGTCTCTTGTTATTCATCCCGCGGAATTTGATCAATAACTTGGAAAGCAAGTTCCAAGCGAGGCAACAGGTGGTGGAAGCAACAAGGGATTCTAGCTCTTCTGGGAACCGGAGCTCCGGGAGTAGGGGGACCACTCCAACTGATAAACCATGGCTAAAACAGCTGAGAGAGTTGGCAAAGTCTAGCTTTAGTAAAGAAGGAAGTAGTGGAGTTGCAAATGATCCTTCGAGTAGTAATACTCGAGCTAGATGGAGTGGGTTTTGGAGCAGCAAAAGGAGTGGGAGCTCATCTCAAAAGTCTTCTAGTGATTCCAAATCAAAATCAAGGTTGTACAATGACTTGGAGGCTATTTTTGCATCCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1082

Amino Acids

119.55

Weight (kDa)

9.16

Isoelectric Point (pI)

45.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Big_12 PF19078 346 - 437 1e-06 Bacterial Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012246)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48200
fragaria_vesca FvH4_5g05550 FvH4_5g05550 FvH4_5g05550
malus_domestica MD06G1171200.v1.1
prunus_persica Prupe.5G170900_v2.0.a1 Prupe.5G170900_v2.0.a1 Prupe.5G170900_v2.0.a1 Prupe.5G170900_v2.0.a1
pyrus_communis pycom06g15260
rosa_chinensis RchiOBHm_Chr7g0193221
rosa_laevigata RLG00000004285
rosa_roxburghii Rroxscaffold_3G00262270
rosa_rugosa Rorug07G0008400
rosa_samantha Rh7AG133900 Rh7BG134900 Rh7CG137900 Rh7DG135900
rosa_wichuraiana Rw7G011450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1842
AarI CACCTGC 1 cut(s) 485
Acc36I ACCTGC 2 cut(s) 485, 492
AccBSI CCGCTC 1 cut(s) 1974
AccII CGCG 1 cut(s) 2905
AciI CCGC 7 cut(s) 444, 923, 1397, 1889, 1974, 2903, 2905
AclWI GGATC 4 cut(s) 592, 1903, 2525, 3098
AcsI RAATTY 9 cut(s) 661, 840, 973, 1115, 1565, 1692, 1861, 2210, 2908
AcuI CTGAAG 4 cut(s) 722, 1695, 1725, 1836
AfaI GTAC 9 cut(s) 257, 354, 678, 1066, 1208, 1917, 2193, 2351, 3215
AfiI CCNNNNNNNGG 5 cut(s) 607, 711, 993, 1133, 3006
AflII CTTAAG 2 cut(s) 95, 2612
AflIII ACRYGT 1 cut(s) 1918
AhdI GACNNNNNGTC 1 cut(s) 2247
AhlI ACTAGT 1 cut(s) 2059
AjiI CACGTC 1 cut(s) 2115
AjnI CCWGG 1 cut(s) 600
AjuI GAANNNNNNNTTGG 6 cut(s) 147, 179, 182, 214, 1626, 1658
AloI GAACNNNNNNTCC 6 cut(s) 1942, 1974, 2918, 2950, 2981, 3013
Alw21I GWGCWC 3 cut(s) 1987, 2999, 3170
Alw26I GTCTC 4 cut(s) 94, 1960, 2102, 2891
AlwI GGATC 4 cut(s) 592, 1903, 2525, 3098
AlwNI CAGNNNCTG 2 cut(s) 368, 818
Ama87I CYCGRG 1 cut(s) 3123
AoxI GGCC 5 cut(s) 1046, 2046, 2203, 2314, 2338
ApeKI GCWGC 4 cut(s) 1402, 2054, 2125, 3150
ApoI RAATTY 9 cut(s) 661, 840, 973, 1115, 1565, 1692, 1861, 2210, 2908
AseI ATTAAT 3 cut(s) 1109, 1230, 2793
Asp700I GAANNNNTTC 1 cut(s) 2849
AspS9I GGNCC 5 cut(s) 2249, 2315, 2339, 2833, 3012
AsuC2I CCSGG 2 cut(s) 2253, 3001
AsuHPI GGTGA 6 cut(s) 761, 970, 1008, 1811, 1903, 2405
AsuII TTCGAA 1 cut(s) 230
AsuNHI GCTAGC 1 cut(s) 176
AvaI CYCGRG 1 cut(s) 3123
AvaII GGWCC 3 cut(s) 2249, 2833, 3012
AxyI CCTNAGG 1 cut(s) 710
BaeGI GKGCMC 1 cut(s) 1335
BaeI ACNNNNGTAYC 2 cut(s) 390, 423
BanII GRGCYC 2 cut(s) 2999, 3170
BbsI GAAGAC 3 cut(s) 697, 1098, 3174
Bbv12I GWGCWC 3 cut(s) 1987, 2999, 3170
BbvCI CCTCAGC 1 cut(s) 2050
BbvI GCAGC 4 cut(s) 1389, 2041, 2112, 3162
BccI CCATC 6 cut(s) 212, 266, 523, 1524, 2538, 3126
BceAI ACGGC 2 cut(s) 457, 2605
BciT130I CCWGG 1 cut(s) 602
BciVI GTATCC 1 cut(s) 2768
BclI TGATCA 1 cut(s) 2913
BcnI CCSGG 2 cut(s) 2253, 3001
BcoDI GTCTC 4 cut(s) 94, 1960, 2102, 2891
BcuI ACTAGT 1 cut(s) 2059
BfmI CTRYAG 2 cut(s) 1050, 1666
BfrI CTTAAG 2 cut(s) 95, 2612
BfuAI ACCTGC 2 cut(s) 485, 492
BfuI GTATCC 1 cut(s) 2768
BglII AGATCT 2 cut(s) 383, 2329
BisI GCNGC 4 cut(s) 1403, 2055, 2126, 3151
BlsI GCNGC 4 cut(s) 1404, 2056, 2127, 3152
Bme1390I CCNGG 3 cut(s) 602, 2253, 3001
Bme18I GGWCC 3 cut(s) 2249, 2833, 3012
BmeRI GACNNNNNGTC 1 cut(s) 2247
BmeT110I CYCGRG 1 cut(s) 3123
BmgBI CACGTC 1 cut(s) 2115
BmgT120I GGNCC 5 cut(s) 2249, 2315, 2339, 2833, 3012
BmiI GGNNCC 6 cut(s) 537, 2250, 2341, 2835, 2990, 3013
BmrFI CCNGG 3 cut(s) 602, 2253, 3001
BmrI ACTGGG 3 cut(s) 252, 1278, 2255
BmsI GCATC 6 cut(s) 374, 449, 1307, 1463, 1475, 2419
BmtI GCTAGC 1 cut(s) 180
BmuI ACTGGG 3 cut(s) 252, 1278, 2255
BpiI GAAGAC 3 cut(s) 697, 1098, 3174
BplI GAGNNNNNCTC 6 cut(s) 1579, 1611, 2642, 2674, 3157, 3189
BpmI CTGGAG 4 cut(s) 554, 984, 1816, 2100
Bpu10I CCTNAGC 1 cut(s) 2050
Bpu14I TTCGAA 1 cut(s) 230
BpuEI CTTGAG 3 cut(s) 294, 364, 1057
BpuMI CCSGG 2 cut(s) 2253, 3001
BsaBI GATNNNNATC 1 cut(s) 463
BsaI GGTCTC 1 cut(s) 94
BsaJI CCNNGG 3 cut(s) 1610, 2903, 3032
BsaWI WCCGGW 2 cut(s) 880, 2991
BsaXI ACNNNNNCTCC 4 cut(s) 2073, 2103, 2981, 3011
Bsc4I CCNNNNNNNGG 5 cut(s) 607, 711, 993, 1133, 3006
Bse21I CCTNAGG 1 cut(s) 710
Bse3DI GCAATG 5 cut(s) 942, 1544, 2383, 2778, 2822
Bse8I GATNNNNATC 1 cut(s) 463
BseBI CCWGG 1 cut(s) 602
BseDI CCNNGG 3 cut(s) 1610, 2903, 3032
BseGI GGATG 7 cut(s) 277, 464, 1279, 1385, 1471, 2898, 3239
BseJI GATNNNNATC 1 cut(s) 463
BseLI CCNNNNNNNGG 5 cut(s) 607, 711, 993, 1133, 3006
BseMI GCAATG 5 cut(s) 942, 1544, 2383, 2778, 2822
BseRI GAGGAG 3 cut(s) 102, 462, 2082
BseSI GKGCMC 1 cut(s) 1335
BseXI GCAGC 4 cut(s) 1389, 2041, 2112, 3162
BseYI CCCAGC 2 cut(s) 1619, 2836
BsgI GTGCAG 4 cut(s) 280, 785, 819, 2040
Bsh1236I CGCG 1 cut(s) 2905
Bsh1285I CGRYCG 1 cut(s) 1870
BshFI GGCC 5 cut(s) 1048, 2048, 2205, 2316, 2340
BsiEI CGRYCG 1 cut(s) 1870
BsiHKAI GWGCWC 3 cut(s) 1987, 2999, 3170
BsiHKCI CYCGRG 1 cut(s) 3123
BsiSI CCGG 4 cut(s) 881, 2252, 2992, 3000
BslFI GGGAC 3 cut(s) 319, 1009, 3025
BslI CCNNNNNNNGG 5 cut(s) 607, 711, 993, 1133, 3006
BsmAI GTCTC 4 cut(s) 94, 1960, 2102, 2891
BsmFI GGGAC 3 cut(s) 319, 1009, 3025
BsmI GAATGC 1 cut(s) 178
BsnI GGCC 5 cut(s) 1048, 2048, 2205, 2316, 2340
Bso31I GGTCTC 1 cut(s) 94
BsoBI CYCGRG 1 cut(s) 3123
Bsp119I TTCGAA 1 cut(s) 230
Bsp1286I GDGCHC 4 cut(s) 1335, 1987, 2999, 3170
Bsp1407I TGTACA 2 cut(s) 1206, 3213
Bsp143I GATC 9 cut(s) 383, 597, 1867, 1908, 2329, 2517, 2652, 2913, 3103
Bsp19I CCATGG 2 cut(s) 1610, 3032
BspACI CCGC 7 cut(s) 444, 923, 1397, 1889, 1974, 2903, 2905
BspANI GGCC 5 cut(s) 1048, 2048, 2205, 2316, 2340
BspFNI CGCG 1 cut(s) 2905
BspHI TCATGA 2 cut(s) 1119, 2751
BspLI GGNNCC 6 cut(s) 537, 2250, 2341, 2835, 2990, 3013
BspMAI CTGCAG 1 cut(s) 1054
BspMI ACCTGC 2 cut(s) 485, 492
BspOI GCTAGC 1 cut(s) 180
BspPI GGATC 4 cut(s) 592, 1903, 2525, 3098
BspQI GCTCTTC 1 cut(s) 2986
BspT104I TTCGAA 1 cut(s) 230
BspTI CTTAAG 2 cut(s) 95, 2612
BspTNI GGTCTC 1 cut(s) 94
BsrBI CCGCTC 1 cut(s) 1974
BsrDI GCAATG 5 cut(s) 942, 1544, 2383, 2778, 2822
BsrGI TGTACA 2 cut(s) 1206, 3213
BssECI CCNNGG 3 cut(s) 1610, 2903, 3032
BssMI GATC 9 cut(s) 383, 597, 1867, 1908, 2329, 2517, 2652, 2913, 3103
BssT1I CCWWGG 2 cut(s) 1610, 3032
Bst2UI CCWGG 1 cut(s) 602
Bst4CI ACNGT 7 cut(s) 72, 343, 352, 763, 958, 1213, 2763
Bst6I CTCTTC 3 cut(s) 1150, 1713, 2986
BstAFI CTTAAG 2 cut(s) 95, 2612
BstAPI GCANNNNNTGC 1 cut(s) 192
BstAUI TGTACA 2 cut(s) 1206, 3213
BstBI TTCGAA 1 cut(s) 230
BstC8I GCNNGC 9 cut(s) 178, 209, 502, 768, 1050, 1423, 1706, 2383, 2387
BstDSI CCRYGG 3 cut(s) 1610, 2903, 3032
BstF5I GGATG 7 cut(s) 277, 464, 1279, 1385, 1471, 2898, 3239
BstFNI CGCG 1 cut(s) 2905
BstKTI GATC 9 cut(s) 386, 600, 1870, 1911, 2332, 2520, 2655, 2916, 3106
BstMAI GTCTC 4 cut(s) 94, 1960, 2102, 2891
BstMBI GATC 9 cut(s) 383, 597, 1867, 1908, 2329, 2517, 2652, 2913, 3103
BstMCI CGRYCG 1 cut(s) 1870
BstNI CCWGG 1 cut(s) 602
BstNSI RCATGY 5 cut(s) 186, 1708, 1879, 1922, 2356
BstSCI CCNGG 3 cut(s) 600, 2251, 2999
BstSFI CTRYAG 2 cut(s) 1050, 1666
BstSLI GKGCMC 1 cut(s) 1335
BstUI CGCG 1 cut(s) 2905
BstV1I GCAGC 4 cut(s) 1389, 2041, 2112, 3162
BstV2I GAAGAC 3 cut(s) 697, 1098, 3174
BstX2I RGATCY 2 cut(s) 383, 2329
BstYI RGATCY 2 cut(s) 383, 2329
Bsu36I CCTNAGG 1 cut(s) 710
BsuI GTATCC 1 cut(s) 2768
BsuRI GGCC 5 cut(s) 1048, 2048, 2205, 2316, 2340
BtgI CCRYGG 3 cut(s) 1610, 2903, 3032
BtrI CACGTC 1 cut(s) 2115
BtsCI GGATG 7 cut(s) 277, 464, 1279, 1385, 1471, 2898, 3239
BtsI GCAGTG 2 cut(s) 731, 1059
BveI ACCTGC 2 cut(s) 485, 492
Cac8I GCNNGC 9 cut(s) 178, 209, 502, 768, 1050, 1423, 1706, 2383, 2387
CaiI CAGNNNCTG 2 cut(s) 368, 818
CciI TCATGA 2 cut(s) 1119, 2751
Cfr13I GGNCC 5 cut(s) 2249, 2315, 2339, 2833, 3012
Cfr42I CCGCGG 1 cut(s) 2906
CseI GACGC 1 cut(s) 2061
Csp6I GTAC 9 cut(s) 256, 353, 677, 1065, 1207, 1916, 2192, 2350, 3214
CspCI CAANNNNNGTGG 2 cut(s) 3142, 3177
CviQI GTAC 9 cut(s) 256, 353, 677, 1065, 1207, 1916, 2192, 2350, 3214
DpnI GATC 9 cut(s) 385, 599, 1869, 1910, 2331, 2519, 2654, 2915, 3105
DpnII GATC 9 cut(s) 383, 597, 1867, 1908, 2329, 2517, 2652, 2913, 3103
DraI TTTAAA 2 cut(s) 846, 2866
DriI GACNNNNNGTC 1 cut(s) 2247
Eam1104I CTCTTC 3 cut(s) 1150, 1713, 2986
Eam1105I GACNNNNNGTC 1 cut(s) 2247
EarI CTCTTC 3 cut(s) 1150, 1713, 2986
EciI GGCGGA 1 cut(s) 459
Ecl136II GAGCTC 2 cut(s) 2997, 3168
Eco130I CCWWGG 2 cut(s) 1610, 3032
Eco147I AGGCCT 2 cut(s) 1048, 2048
Eco24I GRGCYC 2 cut(s) 2999, 3170
Eco31I GGTCTC 1 cut(s) 94
Eco32I GATATC 1 cut(s) 1341
Eco47I GGWCC 3 cut(s) 2249, 2833, 3012
Eco53kI GAGCTC 2 cut(s) 2997, 3168
Eco57I CTGAAG 4 cut(s) 722, 1695, 1725, 1836
Eco81I CCTNAGG 1 cut(s) 710
Eco88I CYCGRG 1 cut(s) 3123
EcoICRI GAGCTC 2 cut(s) 2997, 3168
EcoO109I RGGNCCY 1 cut(s) 2339
EcoRII CCWGG 1 cut(s) 600
EcoRV GATATC 1 cut(s) 1341
EcoT14I CCWWGG 2 cut(s) 1610, 3032
EcoT22I ATGCAT 2 cut(s) 121, 2809
EcoT38I GRGCYC 2 cut(s) 2999, 3170
ErhI CCWWGG 2 cut(s) 1610, 3032
FalI AAGNNNNNCTT 2 cut(s) 3053, 3085
FaqI GGGAC 3 cut(s) 319, 1009, 3025
FauI CCCGC 2 cut(s) 1390, 2910
FbaI TGATCA 1 cut(s) 2913
Fnu4HI GCNGC 4 cut(s) 1403, 2055, 2126, 3151
FokI GGATG 7 cut(s) 284, 471, 1286, 1372, 1458, 2885, 3226
FriOI GRGCYC 2 cut(s) 2999, 3170
Fsp4HI GCNGC 4 cut(s) 1403, 2055, 2126, 3151
GluI GCNGC 4 cut(s) 1403, 2055, 2126, 3151
GsaI CCCAGC 2 cut(s) 1623, 2840
GsuI CTGGAG 4 cut(s) 554, 984, 1816, 2100
HaeIII GGCC 5 cut(s) 1048, 2048, 2205, 2316, 2340
HapII CCGG 4 cut(s) 881, 2252, 2992, 3000
HgaI GACGC 1 cut(s) 2061
HindIII AAGCTT 3 cut(s) 156, 1180, 1627
HpaII CCGG 4 cut(s) 881, 2252, 2992, 3000
HphI GGTGA 6 cut(s) 761, 970, 1008, 1811, 1903, 2405
Hpy166II GTNNAC 5 cut(s) 472, 583, 1209, 1580, 2271
Hpy8I GTNNAC 5 cut(s) 472, 583, 1209, 1580, 2271
HpyCH4III ACNGT 7 cut(s) 72, 343, 352, 763, 958, 1213, 2763
HpyCH4IV ACGT 2 cut(s) 667, 2114
HpySE526I ACGT 2 cut(s) 667, 2114
Ksp22I TGATCA 1 cut(s) 2913
KspI CCGCGG 1 cut(s) 2906
Kzo9I GATC 9 cut(s) 383, 597, 1867, 1908, 2329, 2517, 2652, 2913, 3103
LguI GCTCTTC 1 cut(s) 2986
LmnI GCTCC 9 cut(s) 115, 314, 535, 1982, 2069, 2994, 3002, 3147, 3165
Lsp1109I GCAGC 4 cut(s) 1389, 2041, 2112, 3162
LweI GCATC 6 cut(s) 374, 449, 1307, 1463, 1475, 2419
MaeII ACGT 2 cut(s) 667, 2114
MalI GATC 9 cut(s) 385, 599, 1869, 1910, 2331, 2519, 2654, 2915, 3105
MbiI CCGCTC 1 cut(s) 1974
MboI GATC 9 cut(s) 383, 597, 1867, 1908, 2329, 2517, 2652, 2913, 3103
MfeI CAATTG 2 cut(s) 190, 2151
MflI RGATCY 2 cut(s) 383, 2329
MhlI GDGCHC 4 cut(s) 1335, 1987, 2999, 3170
MlyI GAGTC 3 cut(s) 301, 991, 2402
MmeI TCCRAC 4 cut(s) 729, 1773, 2752, 3044
Mph1103I ATGCAT 2 cut(s) 121, 2809
MroXI GAANNNNTTC 1 cut(s) 2849
MslI CAYNNNNRTG 1 cut(s) 3244
MspA1I CMGCKG 3 cut(s) 2054, 2905, 3046
MspCI CTTAAG 2 cut(s) 95, 2612
MspI CCGG 4 cut(s) 881, 2252, 2992, 3000
MspR9I CCNGG 3 cut(s) 602, 2253, 3001
MunI CAATTG 2 cut(s) 190, 2151
Mva1269I GAATGC 1 cut(s) 178
MvaI CCWGG 1 cut(s) 602
MvnI CGCG 1 cut(s) 2905
NciI CCSGG 2 cut(s) 2253, 3001
NcoI CCATGG 2 cut(s) 1610, 3032
NdeII GATC 9 cut(s) 383, 597, 1867, 1908, 2329, 2517, 2652, 2913, 3103
NheI GCTAGC 1 cut(s) 176
NlaIV GGNNCC 6 cut(s) 537, 2250, 2341, 2835, 2990, 3013
NmeAIII GCCGAG 1 cut(s) 2866
NmuCI GTSAC 4 cut(s) 1363, 2110, 2239, 2726
NsiI ATGCAT 2 cut(s) 121, 2809
NspI RCATGY 5 cut(s) 186, 1708, 1879, 1922, 2356
NspV TTCGAA 1 cut(s) 230
PaeI GCATGC 1 cut(s) 1708
PaeR7I CTCGAG 1 cut(s) 3123
PagI TCATGA 2 cut(s) 1119, 2751
PaqCI CACCTGC 1 cut(s) 485
PceI AGGCCT 2 cut(s) 1048, 2048
PciI ACATGT 1 cut(s) 1918
PciSI GCTCTTC 1 cut(s) 2986
PctI GAATGC 1 cut(s) 178
PdmI GAANNNNTTC 1 cut(s) 2849
PfeI GAWTC 8 cut(s) 431, 645, 715, 968, 1037, 1685, 2972, 3191
PfoI TCCNGGA 2 cut(s) 2251, 2999
PkrI GCNGC 4 cut(s) 1404, 2056, 2127, 3152
Ple19I CGATCG 1 cut(s) 1870
PleI GAGTC 3 cut(s) 301, 991, 2402
PpsI GAGTC 3 cut(s) 301, 991, 2402
PscI ACATGT 1 cut(s) 1918
PshBI ATTAAT 3 cut(s) 1109, 1230, 2793
PsiI TTATAA 1 cut(s) 1842
Psp124BI GAGCTC 2 cut(s) 2999, 3170
Psp6I CCWGG 1 cut(s) 600
PspFI CCCAGC 2 cut(s) 1619, 2836
PspGI CCWGG 1 cut(s) 600
PspN4I GGNNCC 6 cut(s) 537, 2250, 2341, 2835, 2990, 3013
PspPI GGNCC 5 cut(s) 2249, 2315, 2339, 2833, 3012
PspXI VCTCGAGB 1 cut(s) 3123
PstI CTGCAG 1 cut(s) 1054
PstNI CAGNNNCTG 2 cut(s) 368, 818
PsuI RGATCY 2 cut(s) 383, 2329
PvuI CGATCG 1 cut(s) 1870
PvuII CAGCTG 2 cut(s) 2054, 3046
RsaI GTAC 9 cut(s) 257, 354, 678, 1066, 1208, 1917, 2193, 2351, 3215
RsaNI GTAC 9 cut(s) 256, 353, 677, 1065, 1207, 1916, 2192, 2350, 3214
RseI CAYNNNNRTG 1 cut(s) 3244
SacI GAGCTC 2 cut(s) 2999, 3170
SacII CCGCGG 1 cut(s) 2906
SapI GCTCTTC 1 cut(s) 2986
SatI GCNGC 4 cut(s) 1403, 2055, 2126, 3151
Sau3AI GATC 9 cut(s) 383, 597, 1867, 1908, 2329, 2517, 2652, 2913, 3103
Sau96I GGNCC 5 cut(s) 2249, 2315, 2339, 2833, 3012
SchI GAGTC 3 cut(s) 301, 991, 2402
ScrFI CCNGG 3 cut(s) 602, 2253, 3001
SduI GDGCHC 4 cut(s) 1335, 1987, 2999, 3170
SfaNI GCATC 6 cut(s) 374, 449, 1307, 1463, 1475, 2419
SfcI CTRYAG 2 cut(s) 1050, 1666
Sfr274I CTCGAG 1 cut(s) 3123
Sfr303I CCGCGG 1 cut(s) 2906
SfuI TTCGAA 1 cut(s) 230
SgrBI CCGCGG 1 cut(s) 2906
SinI GGWCC 3 cut(s) 2249, 2833, 3012
SlaI CTCGAG 1 cut(s) 3123
SmiMI CAYNNNNRTG 1 cut(s) 3244
SmlI CTYRAG 6 cut(s) 95, 309, 379, 1072, 2612, 3123
SmoI CTYRAG 6 cut(s) 95, 309, 379, 1072, 2612, 3123
SpeI ACTAGT 1 cut(s) 2059
SphI GCATGC 1 cut(s) 1708
SseBI AGGCCT 2 cut(s) 1048, 2048
SsiI CCGC 7 cut(s) 444, 923, 1397, 1889, 1974, 2903, 2905
SspI AATATT 4 cut(s) 418, 529, 551, 1807
SstI GAGCTC 2 cut(s) 2999, 3170
StuI AGGCCT 2 cut(s) 1048, 2048
StyD4I CCNGG 3 cut(s) 600, 2251, 2999
StyI CCWWGG 2 cut(s) 1610, 3032
TaaI ACNGT 7 cut(s) 72, 343, 352, 763, 958, 1213, 2763
TaiI ACGT 2 cut(s) 670, 2117
TaqI TCGA 9 cut(s) 230, 757, 966, 1035, 1847, 1866, 1870, 3110, 3124
TaqII GACCGA 1 cut(s) 1234
TatI WGTACW 7 cut(s) 255, 1064, 1206, 1915, 2191, 2349, 3213
TfiI GAWTC 8 cut(s) 431, 645, 715, 968, 1037, 1685, 2972, 3191
TseFI GTSAC 4 cut(s) 1363, 2110, 2239, 2726
TseI GCWGC 4 cut(s) 1402, 2054, 2125, 3150
Tsp45I GTSAC 4 cut(s) 1363, 2110, 2239, 2726
TspDTI ATGAA 9 cut(s) 9, 707, 1108, 1136, 1578, 1924, 2433, 2740, 2887
Vha464I CTTAAG 2 cut(s) 95, 2612
VpaK11BI GGWCC 3 cut(s) 2249, 2833, 3012
VspI ATTAAT 3 cut(s) 1109, 1230, 2793
XapI RAATTY 9 cut(s) 661, 840, 973, 1115, 1565, 1692, 1861, 2210, 2908
XbaI TCTAGA 1 cut(s) 1526
XceI RCATGY 5 cut(s) 186, 1708, 1879, 1922, 2356
XcmI CCANNNNNNNNNTGG 1 cut(s) 2456
XhoI CTCGAG 1 cut(s) 3123
XmnI GAANNNNTTC 1 cut(s) 2849
Zsp2I ATGCAT 2 cut(s) 121, 2809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.