FvH4_5g06500

Sieve element occlusion C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009415
Physical Location & Seq
Reverse (-)
3821659 .. 3822290
632 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g06500.t1

Sequence Viewer

Length: 540 bp
ATGGCAAAACGTACAGGTATTCCCTTGGAATTGGTTTATGCAGGAAGAAGCACCGCCTCAAAAGAGAAACTCTGGAAACTTAACAGAATTATTGATGCTGAGAATCTCAGTCGCAACTGGTCTGATTACACATCCACCTGGTTCTTTTGGTCTCGACTTGACAGCATGAGGTGCTCAAAGGCTAAGCACCACAAGACTATAGACGATGATACTATACTGAGAGAGGTCATGAGTTTATTTAGCTATGATGGAAGTGACCATGGTTGGGTTATGTTGTGGTTGGGCTCAGCTGATACTGTTAGAGCTAATGGACACCTGACTACTATCACGCTTGACGACTTTGAGGCCTGGAAACCTGCAGCTGCTGAGTTAGGCTTTTTGCCTGAACTCAAAAACCAGCTCAAACAGCTTCACGAGCCACATCATTGTACTCGTTTGATCATTCCTGGGTTCGGTCCAGACATTCCGGACAAAGTGGTGTGTACCGAGTGTGGTCGTGAGATGGAGAAGTTCTTCATGTTTCGCTGCTGCACCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

180

Amino Acids

20.66

Weight (kDa)

6.38

Isoelectric Point (pI)

32.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_C PF14577 2 - 178 8.8e-58 Sieve element occlusion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 364
AccIII TCCGGA 1 cut(s) 466
AciI CCGC 1 cut(s) 54
AfaI GTAC 3 cut(s) 13, 430, 484
AfiI CCNNNNNNNGG 2 cut(s) 265, 452
AjnI CCWGG 3 cut(s) 137, 347, 445
AluBI AGCT 6 cut(s) 243, 290, 305, 362, 400, 409
AluI AGCT 6 cut(s) 243, 290, 305, 362, 400, 409
Alw21I GWGCWC 1 cut(s) 176
Alw26I GTCTC 1 cut(s) 156
AlwNI CAGNNNCTG 1 cut(s) 365
Aor13HI TCCGGA 1 cut(s) 466
AoxI GGCC 1 cut(s) 345
ApeKI GCWGC 4 cut(s) 359, 362, 525, 528
Asp700I GAANNNNTTC 1 cut(s) 512
AspS9I GGNCC 1 cut(s) 455
AvaII GGWCC 1 cut(s) 455
BanII GRGCYC 1 cut(s) 287
BauI CACGAG 1 cut(s) 413
Bbv12I GWGCWC 1 cut(s) 176
BbvI GCAGC 4 cut(s) 349, 371, 512, 515
BccI CCATC 2 cut(s) 242, 496
BciT130I CCWGG 3 cut(s) 139, 349, 447
BclI TGATCA 1 cut(s) 438
BcoDI GTCTC 1 cut(s) 156
BfmI CTRYAG 2 cut(s) 198, 357
BfuAI ACCTGC 1 cut(s) 364
BisI GCNGC 4 cut(s) 360, 363, 526, 529
BlpI GCTNAGC 2 cut(s) 183, 286
BlsI GCNGC 4 cut(s) 361, 364, 527, 530
Bme1390I CCNGG 3 cut(s) 139, 349, 447
Bme18I GGWCC 1 cut(s) 455
BmgT120I GGNCC 1 cut(s) 455
BmrFI CCNGG 3 cut(s) 139, 349, 447
BmsI GCATC 1 cut(s) 85
Bpu1102I GCTNAGC 2 cut(s) 183, 286
BsaI GGTCTC 1 cut(s) 156
BsaJI CCNNGG 3 cut(s) 24, 259, 446
BsaWI WCCGGW 1 cut(s) 466
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 452
Bse1I ACTGG 1 cut(s) 122
BseAI TCCGGA 1 cut(s) 466
BseBI CCWGG 3 cut(s) 139, 349, 447
BseDI CCNNGG 3 cut(s) 24, 259, 446
BseGI GGATG 1 cut(s) 131
BseLI CCNNNNNNNGG 2 cut(s) 265, 452
BseMII CTCAG 5 cut(s) 90, 121, 209, 300, 357
BseNI ACTGG 1 cut(s) 122
BseXI GCAGC 4 cut(s) 349, 371, 512, 515
BsgI GTGCAG 1 cut(s) 514
BshFI GGCC 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 176
BsiSI CCGG 1 cut(s) 467
BslI CCNNNNNNNGG 2 cut(s) 265, 452
BsmAI GTCTC 1 cut(s) 156
BsnI GGCC 1 cut(s) 347
Bso31I GGTCTC 1 cut(s) 156
Bsp1286I GDGCHC 2 cut(s) 176, 287
Bsp13I TCCGGA 1 cut(s) 466
Bsp143I GATC 1 cut(s) 438
Bsp1720I GCTNAGC 2 cut(s) 183, 286
Bsp19I CCATGG 1 cut(s) 259
BspACI CCGC 1 cut(s) 54
BspANI GGCC 1 cut(s) 347
BspCNI CTCAG 5 cut(s) 91, 120, 210, 299, 358
BspEI TCCGGA 1 cut(s) 466
BspHI TCATGA 1 cut(s) 228
BspMAI CTGCAG 1 cut(s) 361
BspMI ACCTGC 1 cut(s) 364
BspTNI GGTCTC 1 cut(s) 156
BsrI ACTGG 1 cut(s) 122
BssECI CCNNGG 3 cut(s) 24, 259, 446
BssMI GATC 1 cut(s) 438
BssSI CACGAG 1 cut(s) 413
BssT1I CCWWGG 2 cut(s) 24, 259
Bst2BI CACGAG 1 cut(s) 413
Bst2UI CCWGG 3 cut(s) 139, 349, 447
Bst4CI ACNGT 1 cut(s) 298
BstAPI GCANNNNNTGC 1 cut(s) 171
BstDEI CTNAG 6 cut(s) 99, 107, 183, 218, 286, 366
BstDSI CCRYGG 1 cut(s) 259
BstF5I GGATG 1 cut(s) 131
BstKTI GATC 1 cut(s) 441
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 1 cut(s) 438
BstMWI GCNNNNNNNGC 3 cut(s) 171, 406, 415
BstNI CCWGG 3 cut(s) 139, 349, 447
BstSCI CCNGG 3 cut(s) 137, 347, 445
BstSFI CTRYAG 2 cut(s) 198, 357
BstV1I GCAGC 4 cut(s) 349, 371, 512, 515
BsuRI GGCC 1 cut(s) 347
BtgI CCRYGG 1 cut(s) 259
BtsCI GGATG 1 cut(s) 131
BveI ACCTGC 1 cut(s) 364
CaiI CAGNNNCTG 1 cut(s) 365
CciI TCATGA 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 455
CsiI ACCWGGT 1 cut(s) 137
Csp6I GTAC 3 cut(s) 12, 429, 483
CviAII CATG 4 cut(s) 166, 229, 260, 517
CviQI GTAC 3 cut(s) 12, 429, 483
DdeI CTNAG 6 cut(s) 99, 107, 183, 218, 286, 366
DpnI GATC 1 cut(s) 440
DpnII GATC 1 cut(s) 438
Eco130I CCWWGG 2 cut(s) 24, 259
Eco147I AGGCCT 1 cut(s) 347
Eco24I GRGCYC 1 cut(s) 287
Eco31I GGTCTC 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 455
EcoRII CCWGG 3 cut(s) 137, 347, 445
EcoT14I CCWWGG 2 cut(s) 24, 259
EcoT38I GRGCYC 1 cut(s) 287
ErhI CCWWGG 2 cut(s) 24, 259
FaeI CATG 4 cut(s) 169, 232, 263, 520
FaiI YATR 9 cut(s) 39, 167, 200, 215, 230, 246, 261, 272, 518
FatI CATG 4 cut(s) 165, 228, 259, 516
FbaI TGATCA 1 cut(s) 438
Fnu4HI GCNGC 4 cut(s) 360, 363, 526, 529
FokI GGATG 1 cut(s) 118
FriOI GRGCYC 1 cut(s) 287
Fsp4HI GCNGC 4 cut(s) 360, 363, 526, 529
GluI GCNGC 4 cut(s) 360, 363, 526, 529
HaeIII GGCC 1 cut(s) 347
HapII CCGG 1 cut(s) 467
Hin1II CATG 4 cut(s) 169, 232, 263, 520
HinfI GANTC 1 cut(s) 103
HpaII CCGG 1 cut(s) 467
Hpy166II GTNNAC 1 cut(s) 483
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 7 cut(s) 73, 153, 229, 413, 458, 467, 497
Hpy8I GTNNAC 1 cut(s) 483
HpyCH4III ACNGT 1 cut(s) 298
HpyCH4IV ACGT 1 cut(s) 10
HpyCH4V TGCA 3 cut(s) 41, 359, 531
HpyF10VI GCNNNNNNNGC 3 cut(s) 171, 406, 415
HpyF3I CTNAG 6 cut(s) 99, 107, 183, 218, 286, 366
HpySE526I ACGT 1 cut(s) 10
Hsp92II CATG 4 cut(s) 169, 232, 263, 520
Kpn2I TCCGGA 1 cut(s) 466
Ksp22I TGATCA 1 cut(s) 438
Kzo9I GATC 1 cut(s) 438
Lsp1109I GCAGC 4 cut(s) 349, 371, 512, 515
LweI GCATC 1 cut(s) 85
MabI ACCWGGT 1 cut(s) 137
MaeII ACGT 1 cut(s) 10
MaeIII GTNAC 1 cut(s) 254
MalI GATC 1 cut(s) 440
MboI GATC 1 cut(s) 438
MboII GAAGA 2 cut(s) 57, 505
MhlI GDGCHC 2 cut(s) 176, 287
MluCI AATT 2 cut(s) 29, 87
MnlI CCTC 4 cut(s) 67, 162, 217, 337
MroI TCCGGA 1 cut(s) 466
MroXI GAANNNNTTC 1 cut(s) 512
MseI TTAA 1 cut(s) 81
MspA1I CMGCKG 2 cut(s) 290, 362
MspI CCGG 1 cut(s) 467
MspR9I CCNGG 3 cut(s) 139, 349, 447
MvaI CCWGG 3 cut(s) 139, 349, 447
MwoI GCNNNNNNNGC 3 cut(s) 171, 406, 415
NcoI CCATGG 1 cut(s) 259
NdeII GATC 1 cut(s) 438
NlaIII CATG 4 cut(s) 169, 232, 263, 520
NmuCI GTSAC 1 cut(s) 254
PagI TCATGA 1 cut(s) 228
PceI AGGCCT 1 cut(s) 347
PdmI GAANNNNTTC 1 cut(s) 512
PfeI GAWTC 1 cut(s) 103
PkrI GCNGC 4 cut(s) 361, 364, 527, 530
Psp6I CCWGG 3 cut(s) 137, 347, 445
PspGI CCWGG 3 cut(s) 137, 347, 445
PspPI GGNCC 1 cut(s) 455
PstI CTGCAG 1 cut(s) 361
PstNI CAGNNNCTG 1 cut(s) 365
PvuII CAGCTG 2 cut(s) 290, 362
RsaI GTAC 3 cut(s) 13, 430, 484
RsaNI GTAC 3 cut(s) 12, 429, 483
SaqAI TTAA 1 cut(s) 81
SatI GCNGC 4 cut(s) 360, 363, 526, 529
Sau3AI GATC 1 cut(s) 438
Sau96I GGNCC 1 cut(s) 455
ScrFI CCNGG 3 cut(s) 139, 349, 447
SduI GDGCHC 2 cut(s) 176, 287
SexAI ACCWGGT 1 cut(s) 137
SfaNI GCATC 1 cut(s) 85
SfcI CTRYAG 2 cut(s) 198, 357
SinI GGWCC 1 cut(s) 455
Sse9I AATT 2 cut(s) 29, 87
SseBI AGGCCT 1 cut(s) 347
SsiI CCGC 1 cut(s) 54
StuI AGGCCT 1 cut(s) 347
StyD4I CCNGG 3 cut(s) 137, 347, 445
StyI CCWWGG 2 cut(s) 24, 259
TaaI ACNGT 1 cut(s) 298
TaiI ACGT 1 cut(s) 13
TaqI TCGA 1 cut(s) 154
TaqII GACCGA 1 cut(s) 443
TasI AATT 2 cut(s) 29, 87
TatI WGTACW 1 cut(s) 428
TfiI GAWTC 1 cut(s) 103
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
TseFI GTSAC 1 cut(s) 254
TseI GCWGC 4 cut(s) 359, 362, 525, 528
Tsp45I GTSAC 1 cut(s) 254
TspDTI ATGAA 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 455
XmnI GAANNNNTTC 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.