Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0009415

Physical Location & Seq

Genomic Coordinates Reverse (-)

26032319 .. 26035659

Gene Structure 3341 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1167500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2295 bp

ATGGCAAGCCGCCAACATCAGCTGACAGCTCCGACGCAGATTCAGTATTCGAATCGGAGGGATAAAAGAACATCATCCTCATCACTATCTGATGATAGTTCATTGGTGAGGCAAATCCGGGACACTCATATTCCAGGAAGATCCGATCACAGCGTCAATGTCAACCAAATTCTCCAAGTTGTCGAGGAAATATTTCATCGCGCTTCTCATGGCCATGCCGGCGTTTTACTTGGTACTCGAGAACATTTAGAGACTGCGGAGGACAAGACCACATTGTCAAGTGCAGATGTCGTGTTCCATGGATTGTCTTATCTCATACAAAAGATCTACTGCGAGATAAGTTGCCAATGCTCAGGCGGCCCTGATGCCCACGCGAGTGCAATCGAGTTACTCAGGACCCTTTCGAGCTACCCATGGGAAGCGAAAGTGGTGCTGACCCTAGCTGCATTTTCTGTGTGCTATGGAGAGTTCTGGCTGGTGGCTCAGCTTTGCACTACCGATCCATTAGCCAAGTCCGTGGCAATCTTGAAGCAACTCTCGGACATTGTAGAGCATGCTGCCTCAGTAAAACCTCAACTTGAGGCAATCGACACCCTCATCAAGGCAATTCTCAATGTCACCAAGCGCATTGTGGAGTACGGCGAGATGGTCAAGATGCAGTCTCGCTACGTTTCAGAAGACACACCACCGCTGTCAATTGCCTTGGCGCATATTCCTGCTGCTACTTATTGGGTCATTCGAGGCATACTGGCTAGTGCCTCCCACATTGCCATCCTTACTGGCAGTAGGCGCGAGTACGTTGCATCAACCACAGAGGTATGGGAACTTTCGAGCTTGGCTCATAAGCTGAACAACATACACGATCACCTCTCGAACGAACTTGAAAATTGTCGCCGACACATTGTGACGAAGAGGTATGATGAAGAATATGAGAATCTGAGACGCCTCTTCCAAAGCCTCCATCTCGACAACGTGAAGAATTTACGCGCACTGATTTCACACAAAGATGAATTTCAACCTATCCAAATCGGCACATTGAAGTCAAGGTTTAGCCTTGAAGTGTTGAGGAGAAAGCATGTGTTGCTGGTAATTACAGATCTTAGTCTGAGCAACGAAGAGATTTTAGTCCTTGATCATATCTACAAAGATCAGCAGAAAAGGCCGGAAATTGAGTGCGAGTTTGTTTGGCTTCCCATAGTGGACACAAATGTTTGGGATGAAGCAAAGCGCTTTAGATTCGAGGACTTGAAGTCAAGGATGCAGTGGTATGCAGTGTATGACCCTCTAATCATCGAACCTCCGGTGGTCAAGTTCGTCAGAAATGATTGGCAATTTGACAAGAAGATGATCGTAGTGGCATTGGATCCACAAGGGAGGGTGTCCTCTCTGAATGCCACCCACATGCTGTGGATTTGGGGCAATGTAGCATACCCTTTCACCGATGAGAGAGAGCAAGTCCTGTGGAATGCAGAGAGCTGGAGGCTTGAGCTCGTTGCCAATGGCATTGATCAAAGCATCCTAGAAGGGATAGAAGGAGGGAGATACATATGCTTGTATGGAAGCGATGACTTAGATTGGATTCGAAAGTTCACAAACAGAGCGAAAAATGTTGCAAAACTCGCCGGCATCTCCTTGGACTTAGTTTACGTAGGAAGAAGCACATCCACCAAGGAGGAGAGGATAAGGAAAGTGAACAAGGTCATCGAAACAGAGAACCTCAGTCGATACTGGCCTGAATACGTGTCAACCTGGTTCTTCTGGTCCCGAATGGACAGCATGCGTTACTCAAGGGCGAAACAGCGCAAGACTCTAGAGAACGATGGCATACTGAAGGAGGTTATGACATTGCTTAGCTATGACGGAAGTGACCAAGGTTGGGTTATGGTGTGGAGAGGCTCAAATGAGACGGCTAGGGCCAACGGACACCTCACTCTACTCACTTTGGATGACTTTGATGCGTGGAAAACCGCAGCTGCAGACTCAGGCTTCGTCCCAACACTCAAAGGTGAACTGCTACGCCGTCACACGCCTCAACACTGCACTCGTTTGGTCATCCCAGGGTTCGGTACTGACATACCGGACAAAATAGCTTGCGCTGAGTGCGGCGGGCCGCGAGATGGAGAAGTTCTTTCATGTTCCGCTGCTGTCTCGATTAAGGCATGTTTACTTAAGCATGATAATGTTGTTGTTAATCATGTTTTATTACATTACTTATGGTGCTCTTTCACCAGAGAGCTATATGTTTTGGCATGCATTAAAAATAAATGTTTGTGTGAACTTGTGAAAAATCGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SEO_C SEO_N

Protein Analysis

765

Amino Acids

87.22

Weight (kDa)

6.73

Isoelectric Point (pI)

45.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SEO_N	PF14576	30 - 321	4.4e-100	Sieve element occlusion N-terminus
SEO_C	PF14577	483 - 703	2.6e-77	Sieve element occlusion C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009415)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01680
fragaria_vesca	FvH4_5g06500 FvH4_5g06680 FvH4_5g06680 FvH4_5g06680
malus_domestica	MD06G1161800.v1.1 MD14G1167500.v1.1 MD14G1167600.v1.1
prunus_persica	Prupe.5G162100_v2.0.a1
pyrus_communis	pycom06g14460 pycom14g13980
rosa_chinensis	RchiOBHm_Chr7g0191981
rosa_laevigata	RLG00000004412
rosa_multiflora	Rmu_ssc0000421.1_g000030
rosa_roxburghii	Rroxscaffold_3G00263390
rosa_rugosa	Rorug06G0512000 Rorug06G0512100
rosa_samantha	Rh7AG122400 Rh7BG124000 Rh7CG126700 Rh7DG125500
rosa_wichuraiana	Rw7G010420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	274
AccII	CGCG	5 cut(s)	201, 374, 792, 987, 2113
AciI	CCGC	9 cut(s)	10, 257, 357, 689, 1968, 2103, 2106, 2111, 2139
AclWI	GGATC	4 cut(s)	135, 494, 1358, 1371
AcoI	YGGCCR	1 cut(s)	211
AcsI	RAATTY	3 cut(s)	168, 979, 1010
AcuI	CTGAAG	1 cut(s)	1850
AcyI	GRCGYC	1 cut(s)	943
AdeI	CACNNNGTG	1 cut(s)	904
AfaI	GTAC	4 cut(s)	235, 638, 797, 2068
AfeI	AGCGCT	1 cut(s)	1229
AfiI	CCNNNNNNNGG	4 cut(s)	601, 1876, 2063, 2117
AflII	CTTAAG	1 cut(s)	2168
AflIII	ACRYGT	1 cut(s)	1740
AgsI	TTSAA	6 cut(s)	529, 884, 1016, 1039, 1058, 1249
AhdI	GACNNNNNGTC	1 cut(s)	1249
AjnI	CCWGG	3 cut(s)	133, 1748, 2056
AleI	CACNNNNGTG	1 cut(s)	375
Alw21I	GWGCWC	2 cut(s)	1491, 2222
Alw26I	GTCTC	5 cut(s)	245, 666, 934, 1898, 2152
AlwI	GGATC	4 cut(s)	135, 494, 1358, 1371
Ama87I	CYCGRG	1 cut(s)	237
Aor51HI	AGCGCT	1 cut(s)	1229
AoxI	GGCC	6 cut(s)	211, 358, 1160, 1730, 1914, 2108
ApeKI	GCWGC	6 cut(s)	443, 557, 719, 1970, 1973, 2141
ApoI	RAATTY	3 cut(s)	168, 979, 1010
ArsI	GACNNNNNNTTYG	2 cut(s)	1970, 2002
Asp700I	GAANNNNTTC	1 cut(s)	192
AspLEI	GCGC	8 cut(s)	203, 627, 709, 792, 989, 1230, 1803, 2096
AspS9I	GGNCC	5 cut(s)	359, 396, 1761, 1914, 2108
AsuC2I	CCSGG	1 cut(s)	119
AsuHPI	GGTGA	6 cut(s)	118, 610, 857, 1429, 2018, 2218
AsuII	TTCGAA	2 cut(s)	50, 1582
AvaI	CYCGRG	1 cut(s)	237
AvaII	GGWCC	2 cut(s)	396, 1761
BalI	TGGCCA	1 cut(s)	213
BamHI	GGATCC	1 cut(s)	1363
BanII	GRGCYC	1 cut(s)	1491
BbsI	GAAGAC	1 cut(s)	684
Bbv12I	GWGCWC	2 cut(s)	1491, 2222
BbvI	GCAGC	6 cut(s)	430, 544, 706, 1960, 1982, 2128
BccI	CCATC	5 cut(s)	640, 779, 969, 1814, 2111
BceAI	ACGGC	3 cut(s)	655, 1923, 2004
BcgI	CGANNNNNNTGC	4 cut(s)	412, 446, 782, 816
BciT130I	CCWGG	3 cut(s)	135, 1750, 2058
BclI	TGATCA	2 cut(s)	1132, 1507
BcnI	CCSGG	1 cut(s)	119
BcoDI	GTCTC	5 cut(s)	245, 666, 934, 1898, 2152
BfaI	CTAG	6 cut(s)	440, 753, 1520, 1811, 1911, 2293
BfmI	CTRYAG	1 cut(s)	1974
BfoI	RGCGCY	1 cut(s)	1231
BfrI	CTTAAG	1 cut(s)	2168
BglI	GCCNNNNNGGC	1 cut(s)	219
BglII	AGATCT	2 cut(s)	324, 1096
BlpI	GCTNAGC	2 cut(s)	483, 1850
Bme1390I	CCNGG	4 cut(s)	119, 135, 1750, 2058
Bme18I	GGWCC	2 cut(s)	396, 1761
BmeRI	GACNNNNNGTC	1 cut(s)	1249
BmeT110I	CYCGRG	1 cut(s)	237
BmgT120I	GGNCC	5 cut(s)	359, 396, 1761, 1914, 2108
BmiI	GGNNCC	3 cut(s)	398, 1365, 1763
BmrFI	CCNGG	4 cut(s)	119, 135, 1750, 2058
BmsI	GCATC	7 cut(s)	355, 645, 812, 1248, 1524, 1635, 1945
BpiI	GAAGAC	1 cut(s)	684
BplI	GAGNNNNNCTC	2 cut(s)	823, 855
BpmI	CTGGAG	1 cut(s)	1498
Bpu10I	CCTNAGC	1 cut(s)	352
Bpu1102I	GCTNAGC	2 cut(s)	483, 1850
Bpu14I	TTCGAA	2 cut(s)	50, 1582
BpuEI	CTTGAG	3 cut(s)	599, 1505, 1771
BpuMI	CCSGG	1 cut(s)	119
BsaAI	YACGTR	2 cut(s)	1648, 1741
BsaHI	GRCGYC	1 cut(s)	943
BsaJI	CCNNGG	9 cut(s)	298, 413, 516, 702, 1632, 1668, 1870, 2056, 2057
BsaWI	WCCGGW	2 cut(s)	1300, 2077
Bsc4I	CCNNNNNNNGG	4 cut(s)	601, 1876, 2063, 2117
Bse118I	RCCGGY	2 cut(s)	218, 1622
Bse1I	ACTGG	3 cut(s)	753, 784, 1733
Bse3DI	GCAATG	3 cut(s)	765, 1426, 1844
BseBI	CCWGG	3 cut(s)	135, 1750, 2058
BseDI	CCNNGG	9 cut(s)	298, 413, 516, 702, 1632, 1668, 1870, 2056, 2057
BseGI	GGATG	8 cut(s)	74, 771, 1222, 1263, 1515, 1661, 1951, 2052
BseLI	CCNNNNNNNGG	4 cut(s)	601, 1876, 2063, 2117
BseMI	GCAATG	3 cut(s)	765, 1426, 1844
BseMII	CTCAG	9 cut(s)	366, 406, 497, 576, 929, 1097, 1732, 1995, 2088
BseNI	ACTGG	3 cut(s)	753, 784, 1733
BseRI	GAGGAG	2 cut(s)	1081, 1688
BseXI	GCAGC	6 cut(s)	430, 544, 706, 1960, 1982, 2128
BsgI	GTGCAG	2 cut(s)	303, 2023
Bsh1236I	CGCG	5 cut(s)	201, 374, 792, 987, 2113
BshFI	GGCC	6 cut(s)	213, 360, 1162, 1732, 1916, 2110
BsiHKAI	GWGCWC	2 cut(s)	1491, 2222
BsiHKCI	CYCGRG	1 cut(s)	237
BsiSI	CCGG	6 cut(s)	118, 219, 1163, 1301, 1623, 2078
BslFI	GGGAC	3 cut(s)	134, 1747, 1976
BslI	CCNNNNNNNGG	4 cut(s)	601, 1876, 2063, 2117
BsmAI	GTCTC	5 cut(s)	245, 666, 934, 1898, 2152
BsmBI	CGTCTC	2 cut(s)	934, 1898
BsmFI	GGGAC	3 cut(s)	134, 1747, 1976
BsmI	GAATGC	2 cut(s)	1396, 1471
BsnI	GGCC	6 cut(s)	213, 360, 1162, 1732, 1916, 2110
BsoBI	CYCGRG	1 cut(s)	237
Bsp119I	TTCGAA	2 cut(s)	50, 1582
Bsp1286I	GDGCHC	2 cut(s)	1491, 2222
Bsp1720I	GCTNAGC	2 cut(s)	483, 1850
Bsp19I	CCATGG	2 cut(s)	298, 413
BspACI	CCGC	9 cut(s)	10, 257, 357, 689, 1968, 2103, 2106, 2111, 2139
BspANI	GGCC	6 cut(s)	213, 360, 1162, 1732, 1916, 2110
BspCNI	CTCAG	9 cut(s)	365, 405, 496, 575, 930, 1098, 1731, 1994, 2089
BspFNI	CGCG	5 cut(s)	201, 374, 792, 987, 2113
BspLI	GGNNCC	3 cut(s)	398, 1365, 1763
BspMAI	CTGCAG	1 cut(s)	1978
BspPI	GGATC	4 cut(s)	135, 494, 1358, 1371
BspT104I	TTCGAA	2 cut(s)	50, 1582
BspTI	CTTAAG	1 cut(s)	2168
BsrDI	GCAATG	3 cut(s)	765, 1426, 1844
BsrFI	RCCGGY	2 cut(s)	218, 1622
BsrI	ACTGG	3 cut(s)	753, 784, 1733
BssAI	RCCGGY	2 cut(s)	218, 1622
BssECI	CCNNGG	9 cut(s)	298, 413, 516, 702, 1632, 1668, 1870, 2056, 2057
BssNI	GRCGYC	1 cut(s)	943
BssT1I	CCWWGG	6 cut(s)	298, 413, 702, 1632, 1668, 1870
Bst2UI	CCWGG	3 cut(s)	135, 1750, 2058
Bst6I	CTCTTC	3 cut(s)	905, 953, 1110
BstACI	GRCGYC	1 cut(s)	943
BstAFI	CTTAAG	1 cut(s)	2168
BstAPI	GCANNNNNTGC	1 cut(s)	1081
BstBAI	YACGTR	2 cut(s)	1648, 1741
BstBI	TTCGAA	2 cut(s)	50, 1582
BstC8I	GCNNGC	8 cut(s)	7, 220, 555, 1624, 1778, 2092, 2108, 2251
BstDSI	CCRYGG	3 cut(s)	298, 413, 516
BstENI	CCTNNNNNAGG	1 cut(s)	599
BstF5I	GGATG	8 cut(s)	74, 771, 1222, 1263, 1515, 1661, 1951, 2052
BstFNI	CGCG	5 cut(s)	201, 374, 792, 987, 2113
BstH2I	RGCGCY	1 cut(s)	1231
BstHHI	GCGC	8 cut(s)	203, 627, 709, 792, 989, 1230, 1803, 2096
BstMAI	GTCTC	5 cut(s)	245, 666, 934, 1898, 2152
BstMWI	GCNNNNNNNGC	7 cut(s)	219, 357, 789, 1081, 1159, 1619, 2100
BstNI	CCWGG	3 cut(s)	135, 1750, 2058
BstNSI	RCATGY	6 cut(s)	557, 1079, 1405, 1780, 2163, 2253
BstSCI	CCNGG	4 cut(s)	117, 133, 1748, 2056
BstSFI	CTRYAG	1 cut(s)	1974
BstSNI	TACGTA	1 cut(s)	1648
BstUI	CGCG	5 cut(s)	201, 374, 792, 987, 2113
BstV1I	GCAGC	6 cut(s)	430, 544, 706, 1960, 1982, 2128
BstV2I	GAAGAC	1 cut(s)	684
BstX2I	RGATCY	4 cut(s)	140, 324, 1096, 1363
BstXI	CCANNNNNNTGG	1 cut(s)	517
BstYI	RGATCY	4 cut(s)	140, 324, 1096, 1363
BsuRI	GGCC	6 cut(s)	213, 360, 1162, 1732, 1916, 2110
BtgI	CCRYGG	3 cut(s)	298, 413, 516
BtgZI	GCGATG	2 cut(s)	182, 1578
BtsCI	GGATG	8 cut(s)	74, 771, 1222, 1263, 1515, 1661, 1951, 2052
BtsI	GCAGTG	3 cut(s)	1268, 1278, 2035
BtsIMutI	CAGTG	4 cut(s)	989, 1268, 1278, 2035
Cac8I	GCNNGC	8 cut(s)	7, 220, 555, 1624, 1778, 2092, 2108, 2251
CfoI	GCGC	8 cut(s)	203, 627, 709, 792, 989, 1230, 1803, 2096
Cfr10I	RCCGGY	2 cut(s)	218, 1622
Cfr13I	GGNCC	5 cut(s)	359, 396, 1761, 1914, 2108
CseI	GACGC	3 cut(s)	43, 142, 951
CsiI	ACCWGGT	1 cut(s)	1748
Csp6I	GTAC	4 cut(s)	234, 637, 796, 2067
CspCI	CAANNNNNGTGG	2 cut(s)	1442, 1477
CviQI	GTAC	4 cut(s)	234, 637, 796, 2067
DraIII	CACNNNGTG	1 cut(s)	904
DrdI	GACNNNNNNGTC	1 cut(s)	274
DriI	GACNNNNNGTC	1 cut(s)	1249
DseDI	GACNNNNNNGTC	1 cut(s)	274
EaeI	YGGCCR	1 cut(s)	211
Eam1104I	CTCTTC	3 cut(s)	905, 953, 1110
Eam1105I	GACNNNNNGTC	1 cut(s)	1249
EarI	CTCTTC	3 cut(s)	905, 953, 1110
Ecl136II	GAGCTC	1 cut(s)	1489
Eco105I	TACGTA	1 cut(s)	1648
Eco130I	CCWWGG	6 cut(s)	298, 413, 702, 1632, 1668, 1870
Eco24I	GRGCYC	1 cut(s)	1491
Eco47I	GGWCC	2 cut(s)	396, 1761
Eco47III	AGCGCT	1 cut(s)	1229
Eco53kI	GAGCTC	1 cut(s)	1489
Eco57I	CTGAAG	1 cut(s)	1850
Eco88I	CYCGRG	1 cut(s)	237
EcoICRI	GAGCTC	1 cut(s)	1489
EcoNI	CCTNNNNNAGG	1 cut(s)	599
EcoO109I	RGGNCCY	1 cut(s)	396
EcoRII	CCWGG	3 cut(s)	133, 1748, 2056
EcoT14I	CCWWGG	6 cut(s)	298, 413, 702, 1632, 1668, 1870
EcoT22I	ATGCAT	1 cut(s)	2255
EcoT38I	GRGCYC	1 cut(s)	1491
ErhI	CCWWGG	6 cut(s)	298, 413, 702, 1632, 1668, 1870
Esp3I	CGTCTC	2 cut(s)	934, 1898
FaqI	GGGAC	3 cut(s)	134, 1747, 1976
FauI	CCCGC	1 cut(s)	2099
FauNDI	CATATG	1 cut(s)	1547
FbaI	TGATCA	2 cut(s)	1132, 1507
FokI	GGATG	8 cut(s)	61, 758, 1229, 1270, 1502, 1648, 1958, 2039
FriOI	GRGCYC	1 cut(s)	1491
FspBI	CTAG	6 cut(s)	440, 753, 1520, 1811, 1911, 2293
GlaI	GCGC	8 cut(s)	202, 626, 708, 791, 988, 1229, 1802, 2095
GsuI	CTGGAG	1 cut(s)	1498
HaeII	RGCGCY	1 cut(s)	1231
HaeIII	GGCC	6 cut(s)	213, 360, 1162, 1732, 1916, 2110
HapII	CCGG	6 cut(s)	118, 219, 1163, 1301, 1623, 2078
HgaI	GACGC	3 cut(s)	43, 142, 951
HhaI	GCGC	8 cut(s)	203, 627, 709, 792, 989, 1230, 1803, 2096
Hin1I	GRCGYC	1 cut(s)	943
Hin6I	GCGC	8 cut(s)	201, 625, 707, 790, 987, 1228, 1801, 2094
HinP1I	GCGC	8 cut(s)	201, 625, 707, 790, 987, 1228, 1801, 2094
HincII	GTYRAC	2 cut(s)	163, 1746
HindII	GTYRAC	2 cut(s)	163, 1746
HinfI	GANTC	7 cut(s)	40, 52, 934, 1236, 1579, 1807, 1979
HpaII	CCGG	6 cut(s)	118, 219, 1163, 1301, 1623, 2078
HphI	GGTGA	6 cut(s)	118, 610, 857, 1429, 2018, 2218
Hpy166II	GTNNAC	9 cut(s)	163, 1201, 1590, 1645, 1693, 1746, 2009, 2165, 2276
Hpy188III	TCNNGA	9 cut(s)	239, 394, 526, 652, 871, 965, 1764, 1811, 2149
Hpy8I	GTNNAC	9 cut(s)	163, 1201, 1590, 1645, 1693, 1746, 2009, 2165, 2276
Hpy99I	CGWCG	1 cut(s)	37
HpyAV	CCTTC	3 cut(s)	1517, 1526, 1825
HpyCH4IV	ACGT	5 cut(s)	669, 798, 972, 1647, 1740
HpyF10VI	GCNNNNNNNGC	7 cut(s)	219, 357, 789, 1081, 1159, 1619, 2100
HpySE526I	ACGT	5 cut(s)	669, 798, 972, 1647, 1740
Hsp92I	GRCGYC	1 cut(s)	943
HspAI	GCGC	8 cut(s)	201, 625, 707, 790, 987, 1228, 1801, 2094
KroI	GCCGGC	2 cut(s)	218, 1622
KroNI	GCCGGC	2 cut(s)	220, 1624
Ksp22I	TGATCA	2 cut(s)	1132, 1507
LmnI	GCTCC	1 cut(s)	34
Lsp1109I	GCAGC	6 cut(s)	430, 544, 706, 1960, 1982, 2128
LweI	GCATC	7 cut(s)	355, 645, 812, 1248, 1524, 1635, 1945
MabI	ACCWGGT	1 cut(s)	1748
MaeI	CTAG	6 cut(s)	440, 753, 1520, 1811, 1911, 2293
MaeII	ACGT	5 cut(s)	669, 798, 972, 1647, 1740
MaeIII	GTNAC	6 cut(s)	387, 616, 904, 1781, 1865, 2021
MfeI	CAATTG	1 cut(s)	696
MflI	RGATCY	4 cut(s)	140, 324, 1096, 1363
MhlI	GDGCHC	2 cut(s)	1491, 2222
MlsI	TGGCCA	1 cut(s)	213
MluCI	AATT	9 cut(s)	168, 606, 696, 886, 979, 1010, 1089, 1167, 1331
MluNI	TGGCCA	1 cut(s)	213
MlyI	GAGTC	2 cut(s)	1801, 1973
MmeI	TCCRAC	1 cut(s)	56
Mox20I	TGGCCA	1 cut(s)	213
Mph1103I	ATGCAT	1 cut(s)	2255
MroNI	GCCGGC	2 cut(s)	218, 1622
MroXI	GAANNNNTTC	1 cut(s)	192
MscI	TGGCCA	1 cut(s)	213
MseI	TTAA	4 cut(s)	2154, 2169, 2190, 2256
MslI	CAYNNNNRTG	5 cut(s)	213, 375, 1005, 1400, 2178
Msp20I	TGGCCA	1 cut(s)	213
MspA1I	CMGCKG	4 cut(s)	22, 691, 1973, 2141
MspCI	CTTAAG	1 cut(s)	2168
MspI	CCGG	6 cut(s)	118, 219, 1163, 1301, 1623, 2078
MspR9I	CCNGG	4 cut(s)	119, 135, 1750, 2058
MunI	CAATTG	1 cut(s)	696
Mva1269I	GAATGC	2 cut(s)	1396, 1471
MvaI	CCWGG	3 cut(s)	135, 1750, 2058
MvnI	CGCG	5 cut(s)	201, 374, 792, 987, 2113
MwoI	GCNNNNNNNGC	7 cut(s)	219, 357, 789, 1081, 1159, 1619, 2100
NaeI	GCCGGC	2 cut(s)	220, 1624
NciI	CCSGG	1 cut(s)	119
NcoI	CCATGG	2 cut(s)	298, 413
NdeI	CATATG	1 cut(s)	1547
NgoMIV	GCCGGC	2 cut(s)	218, 1622
NlaIV	GGNNCC	3 cut(s)	398, 1365, 1763
NmuCI	GTSAC	4 cut(s)	616, 904, 1865, 2021
NsiI	ATGCAT	1 cut(s)	2255
NspI	RCATGY	6 cut(s)	557, 1079, 1405, 1780, 2163, 2253
NspV	TTCGAA	2 cut(s)	50, 1582
OliI	CACNNNNGTG	1 cut(s)	375
PaeI	GCATGC	3 cut(s)	557, 1780, 2253
PaeR7I	CTCGAG	1 cut(s)	237
PasI	CCCWGGG	1 cut(s)	2057
PctI	GAATGC	2 cut(s)	1396, 1471
PdiI	GCCGGC	2 cut(s)	220, 1624
PdmI	GAANNNNTTC	1 cut(s)	192
PfeI	GAWTC	5 cut(s)	40, 52, 934, 1236, 1579
PfoI	TCCNGGA	2 cut(s)	117, 133
PleI	GAGTC	2 cut(s)	1801, 1973
PpsI	GAGTC	2 cut(s)	1801, 1973
Ppu21I	YACGTR	2 cut(s)	1648, 1741
PpuMI	RGGWCCY	1 cut(s)	396
Psp124BI	GAGCTC	1 cut(s)	1491
Psp5II	RGGWCCY	1 cut(s)	396
Psp6I	CCWGG	3 cut(s)	133, 1748, 2056
PspGI	CCWGG	3 cut(s)	133, 1748, 2056
PspN4I	GGNNCC	3 cut(s)	398, 1365, 1763
PspPI	GGNCC	5 cut(s)	359, 396, 1761, 1914, 2108
PspPPI	RGGWCCY	1 cut(s)	396
PstI	CTGCAG	1 cut(s)	1978
PsuI	RGATCY	4 cut(s)	140, 324, 1096, 1363
PvuII	CAGCTG	2 cut(s)	22, 1973
RsaI	GTAC	4 cut(s)	235, 638, 797, 2068
RsaNI	GTAC	4 cut(s)	234, 637, 796, 2067
RseI	CAYNNNNRTG	5 cut(s)	213, 375, 1005, 1400, 2178
SacI	GAGCTC	1 cut(s)	1491
SaqAI	TTAA	4 cut(s)	2154, 2169, 2190, 2256
Sau96I	GGNCC	5 cut(s)	359, 396, 1761, 1914, 2108
SchI	GAGTC	2 cut(s)	1801, 1973
ScrFI	CCNGG	4 cut(s)	119, 135, 1750, 2058
SduI	GDGCHC	2 cut(s)	1491, 2222
SexAI	ACCWGGT	1 cut(s)	1748
SfaNI	GCATC	7 cut(s)	355, 645, 812, 1248, 1524, 1635, 1945
SfcI	CTRYAG	1 cut(s)	1974
Sfr274I	CTCGAG	1 cut(s)	237
SfuI	TTCGAA	2 cut(s)	50, 1582
SinI	GGWCC	2 cut(s)	396, 1761
SlaI	CTCGAG	1 cut(s)	237
SmiMI	CAYNNNNRTG	5 cut(s)	213, 375, 1005, 1400, 2178
SmlI	CTYRAG	5 cut(s)	237, 578, 1484, 1786, 2168
SmoI	CTYRAG	5 cut(s)	237, 578, 1484, 1786, 2168
SnaBI	TACGTA	1 cut(s)	1648
SphI	GCATGC	3 cut(s)	557, 1780, 2253
Sse9I	AATT	9 cut(s)	168, 606, 696, 886, 979, 1010, 1089, 1167, 1331
SsiI	CCGC	9 cut(s)	10, 257, 357, 689, 1968, 2103, 2106, 2111, 2139
SspI	AATATT	1 cut(s)	192
SspMI	CTAG	6 cut(s)	440, 753, 1520, 1811, 1911, 2293
SstI	GAGCTC	1 cut(s)	1491
StyD4I	CCNGG	4 cut(s)	117, 133, 1748, 2056
StyI	CCWWGG	6 cut(s)	298, 413, 702, 1632, 1668, 1870
TaiI	ACGT	5 cut(s)	672, 801, 975, 1650, 1743
TasI	AATT	9 cut(s)	168, 606, 696, 886, 979, 1010, 1089, 1167, 1331
TauI	GCSGC	4 cut(s)	12, 360, 2106, 2113
TfiI	GAWTC	5 cut(s)	40, 52, 934, 1236, 1579
Tru1I	TTAA	4 cut(s)	2154, 2169, 2190, 2256
Tru9I	TTAA	4 cut(s)	2154, 2169, 2190, 2256
TscAI	CASTG	4 cut(s)	996, 1268, 1278, 2042
TseFI	GTSAC	4 cut(s)	616, 904, 1865, 2021
TseI	GCWGC	6 cut(s)	443, 557, 719, 1970, 1973, 2141
Tsp45I	GTSAC	4 cut(s)	616, 904, 1865, 2021
TspDTI	ATGAA	6 cut(s)	90, 185, 936, 1023, 1233, 2121
TspGWI	ACGGA	3 cut(s)	505, 1875, 1935
TspRI	CASTG	4 cut(s)	996, 1268, 1278, 2042
Vha464I	CTTAAG	1 cut(s)	2168
VpaK11BI	GGWCC	2 cut(s)	396, 1761
XagI	CCTNNNNNAGG	1 cut(s)	599
XapI	RAATTY	3 cut(s)	168, 979, 1010
XbaI	TCTAGA	1 cut(s)	1810
XceI	RCATGY	6 cut(s)	557, 1079, 1405, 1780, 2163, 2253
XcmI	CCANNNNNNNNNTGG	1 cut(s)	628
XhoI	CTCGAG	1 cut(s)	237
XmnI	GAANNNNTTC	1 cut(s)	192
XspI	CTAG	6 cut(s)	440, 753, 1520, 1811, 1911, 2293
Zsp2I	ATGCAT	1 cut(s)	2255

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.