Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0016533

Physical Location & Seq

Genomic Coordinates Forward (+)

8228202 .. 8230880

Gene Structure 2679 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g14520.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2679 bp

ATGGGGAGAGTGACAAATGGGGCATCATCACATGTTCCTCCACAACTCTCCCCCTTCTCCCTTCTTTTCCTTCTCTTCCTGTTCACATTCTTCACCAATCTCATGCACATTGTCTCAGCCAAAGGCCATTCACATTCAAATTCTGGTACGTTGACATTCACTCCTTCTGATAATTTCCTCATCGACTGTGGCGCTCCCCAAGAAGCCAAGCTCCCTGATGGCAGGACGTTCAAATCGGACCGCGAAACAGCTGACCTCTTGTCCACAAATGAGGACATTAAGGCTTCAGCTGACTCCATCAATGCCAATGTCTCTTCATCTGTTCCACCTGATTCAAAAGTTCTGTTTCAAACGGCGAGGATCTTCTCCGAGGATTCCTTGTACACATTCCACATTAACCAGCCCGGCCGCCATTGGATTCGCCTCTACTTCTATCCTCTCCCGCATCAGACATACAACCTTTCCGACGCAGTTTTCTCGGTGCAGACAGACAAGTATGTTCTCTTGCACGACTTCTCTGCCTCGGATAAAGCCACATTGGTGTTCAAAGAATACCTTTTCAATGTCTCTGACACCAGATTCTCCCTCCATTTCAAACCAAAGAAGAAGTCCATTGCTTTTGTCAATGCCATTGAGGTTGTCTCTGCTCCTGACGCTATCTTCAATGACTCTGCCACCACTCTTCCCATATCCAACGACTTCAACGGCTTGCAAGACTTTGCTTTCCAAATCCGGTACCGGCTAAACGTTGGAGGGCCATTGATAACACCTCAAAATGACACTTTGCAAAGGACTTGGGAGCCAGATACTGCATACAATGCATTTCCACAGGGATCTCAAAACGTCTCAGTGGCTCCGAATATCGTCAAGTATCCCAACAGTGGAGCTAACTCGTTGATTGCGCCGAATTGGATCTACGCAACTGCTCAACACATGCAGGATTCTTTAACAAACAACCAGAATTTCAACCTCAGTTGGAAGCTGAGTGTTGAGGAGGACTTTTCCTACTTGATCAGGTTACATTTCTGTGACATACTGAGCAAAACCCTTAATAACTTGTACTTCAATGTGTACATCAATGGCATGGTGGCTGTCTCCAACCTTGATCTCTCTTCACTCACCGGCGGGCTAGCCACTGCTTTTTACAAAGACTTTTTGCTCAATTCCACGGCGGTCTCCATCTTAAACAACACCATCCACATTCAGATTGGTCCTGGCACTACTCAGTCCGGGACTCATGATGGACTGCTTAATGGAGTAGAGGTCTTGAAACTGAGCAATATAGCAGAAAGCTTGGATGGATTTTTCGCCGTTGATGGGAAATACAAGGGTCCGGTGAAATCATCAAAGACATTGAAGATAGCAGCGGGTGTGGGATTGGGAATGGGAGTGACGGCAATGTTCTTACTTGGCGCGGTCTATATAAGGTGGAAAAGGAGACCTCAAGGTTGGGAAAAGAAAGACAGCTTCTCATCATGGTTGCTCCCTTTACATTCTGGTAACTCTAGATTGTTCTCCAAGTCATGCAAGGGTTCTTCTAGACGATCAAGCAGCGTTTTCGGCTCACGCAAGAGCAAGAGTGGCTACTCAACTTATCTCTCTTCCATTTACTACGGCAGGTCTTTCACTTTCGCTGAGTTACAAGCTGCAACTCAGAATTGGGATGAGAAGGAGGTGATCGGAGTTGGGGGGTTCGGGAAAGTGTACCTTGGGGTGTTGGAAGATGGTACTAAGCTTGCTGTCAAACGAGGGAACCCCAACTCTGAGCAAGGCATTAATGAGTTCAGAACAGAAATGGACATGCTCTCCAAGCTAAGACATCGCCACCTTGTTTCACTGATTGGATTTTGTGATGAGAATAATGAGATGATACTGGTGTACGAGTACATGTCTAATGGACCCCTTCGAGACCACCTCTATGGCTCGAACCTGCTTTCATTGTCGTGGAAGCAAAGGCTCGAGGTTTGCATTGGCGCTGCTCGCGGCTTGCATTATCTGCATACTGGTGCAGCACAAGGCATCATCCATCGTGATGTCAAGACGACGAATATCCTCCTGGATGAGAATTTTGTTGCGAAAGTCGCTGATTTCGGACTGTCGAAAGCTGCTCCATCGTTGGAACAAACGCATGTCAGCACAGCTGTGAAGGGAAGTTTTGGTTATCTTGATCCTGAGTACTTCAGAAGGCAGCAGCTTACTGAGAAATCCGATGTCTACTCTTTCGGTGTGGTTCTTTTCGAGGCATTATGTGCAAGGCCTGTTATATGCCCCACTCTGCCAAGAGAGCAGGTGAGTTTGGCCGAGTGGACAATGCAGTGGCATAGGAAAGGCATGATAGAGAAGATTGTAGACCCTAAAATCGCAAGCTCAATCAACGCAGGTTCGCTGAAGAAGTATGTAGAAGCTGCAGAGAAATGCCTTGCGGAGTATGGTGTCGATAGGCCTAGCATGGGAGATGTGCTGTGGAACTTGGAGTATGCTTTGCAGCTTCAAGAGACAGCCTCACAGATTGATGCTCAGGAAGACAATACTTCGAGTCTCATTTCTTTGGATAAAGCAAGCGAAAAAGATGAGACAAACACAAAGGATGGCTCTGCGGTTGGTAGTGCAGTGAGCGAGGATTCTGTAGTCACAGCTGGTAATCCTTTGTTTTCTCAAATGAATGTGCAGGGGAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009705 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019725 GO:0021700 GO:0022622 GO:0030154 GO:0031090 GO:0032501 GO:0032502 GO:0032879 GO:0036211 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048364 GO:0048468 GO:0048469 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0051049 GO:0051924 GO:0055082 GO:0065007 GO:0065008 GO:0071695 GO:0071704 GO:0080147 GO:0090558 GO:0090627 GO:0097272 GO:0097275 GO:0098588 GO:0098805 GO:0099402 GO:0140096 GO:1901564 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LON_substr_bdg Malectin_like Pkinase_Tyr zf-C3HC4_2

Protein Analysis

893

Amino Acids

98.7

Weight (kDa)

6.53

Isoelectric Point (pI)

34.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin	PF11721	60 - 203	3.7e-06	Malectin domain
Malectin_like	PF12819	61 - 424	5.6e-29	Malectin-like domain
Malectin	PF11721	247 - 399	9.1e-10	Malectin domain
Pkinase	PF00069	555 - 817	3.4e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	557 - 770	4.6e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0016533)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61350
fragaria_vesca	FvH4_5g14520
malus_domestica	MD14G1205200.v1.1
prunus_persica	Prupe.5G198600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0182131
rosa_laevigata	RLG00000005148
rosa_multiflora	Rmu_co8484825.1_g000001
rosa_roxburghii	Rroxscaffold_3G00271480
rosa_rugosa	Rorug06G0445800
rosa_samantha	Rh7AG048600 Rh7BG048400 Rh7CG049900 Rh7DG047800
rosa_wichuraiana	Rw7G003970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2278
Acc36I	ACCTGC	4 cut(s)	1608, 1938, 2278, 2369
Acc65I	GGTACC	1 cut(s)	735
AccB1I	GGYRCC	1 cut(s)	735
AccI	GTMKAC	2 cut(s)	2214, 2349
AccII	CGCG	3 cut(s)	243, 1415, 1983
AclI	AACGTT	1 cut(s)	747
AclWI	GGATC	4 cut(s)	368, 841, 920, 2162
AcoI	YGGCCR	2 cut(s)	406, 2298
AcsI	RAATTY	3 cut(s)	139, 961, 2065
AcuI	CTGAAG	3 cut(s)	270, 2164, 2408
AfiI	CCNNNNNNNGG	3 cut(s)	881, 1317, 2450
AflIII	ACRYGT	2 cut(s)	31, 1887
AhdI	GACNNNNNGTC	1 cut(s)	259
AjnI	CCWGG	2 cut(s)	1213, 2055
AleI	CACNNNNGTG	2 cut(s)	539, 2141
AlwI	GGATC	4 cut(s)	368, 841, 920, 2162
AlwNI	CAGNNNCTG	1 cut(s)	809
Ama87I	CYCGRG	1 cut(s)	1958
AoxI	GGCC	6 cut(s)	124, 406, 755, 2255, 2298, 2441
ApoI	RAATTY	3 cut(s)	139, 961, 2065
ArsI	GACNNNNNNTTYG	2 cut(s)	593, 625
AseI	ATTAAT	1 cut(s)	1776
Asp718I	GGTACC	1 cut(s)	735
AspLEI	GCGC	4 cut(s)	194, 904, 1415, 1976
AspS9I	GGNCC	5 cut(s)	238, 755, 1211, 1331, 1898
AsuC2I	CCSGG	2 cut(s)	405, 1231
AsuHPI	GGTGA	5 cut(s)	85, 1111, 1348, 1687, 2302
AsuNHI	GCTAGC	1 cut(s)	1129
AvaI	CYCGRG	1 cut(s)	1958
AvaII	GGWCC	4 cut(s)	238, 1211, 1331, 1898
BaeI	ACNNNNGTAYC	2 cut(s)	1862, 1895
BanI	GGYRCC	1 cut(s)	735
BbsI	GAAGAC	1 cut(s)	2529
BceAI	ACGGC	6 cut(s)	369, 721, 1185, 1295, 1410, 1630
BcgI	CGANNNNNNTGC	6 cut(s)	459, 493, 500, 534, 1540, 1574
BciT130I	CCWGG	2 cut(s)	1215, 2057
BciVI	GTATCC	1 cut(s)	882
BclI	TGATCA	1 cut(s)	1011
BcnI	CCSGG	2 cut(s)	405, 1231
BfaI	CTAG	4 cut(s)	1130, 1506, 1539, 2445
BfmI	CTRYAG	2 cut(s)	2406, 2625
BfoI	RGCGCY	2 cut(s)	195, 1977
BfuAI	ACCTGC	4 cut(s)	1608, 1938, 2278, 2369
BfuI	GTATCC	1 cut(s)	882
BmcAI	AGTACT	1 cut(s)	2177
Bme1390I	CCNGG	4 cut(s)	405, 1215, 1231, 2057
Bme18I	GGWCC	4 cut(s)	238, 1211, 1331, 1898
BmeRI	GACNNNNNGTC	1 cut(s)	259
BmeT110I	CYCGRG	1 cut(s)	1958
BmgT120I	GGNCC	5 cut(s)	238, 755, 1211, 1331, 1898
BmiI	GGNNCC	6 cut(s)	737, 801, 855, 1332, 1754, 1900
BmrFI	CCNGG	4 cut(s)	405, 1215, 1231, 2057
BmsI	GCATC	4 cut(s)	32, 454, 2028, 2503
BmtI	GCTAGC	1 cut(s)	1133
BpiI	GAAGAC	1 cut(s)	2529
BplI	GAGNNNNNCTC	6 cut(s)	626, 658, 1899, 1931, 2486, 2518
Bpu10I	CCTNAGC	1 cut(s)	2517
BpuEI	CTTGAG	1 cut(s)	1428
BpuMI	CCSGG	2 cut(s)	405, 1231
BsaI	GGTCTC	3 cut(s)	1180, 1432, 1902
BsaJI	CCNNGG	4 cut(s)	369, 522, 1167, 1708
BsaWI	WCCGGW	2 cut(s)	732, 1333
BsaXI	ACNNNNNCTCC	6 cut(s)	145, 175, 1248, 1278, 1790, 1820
Bsc4I	CCNNNNNNNGG	3 cut(s)	881, 1317, 2450
Bse118I	RCCGGY	2 cut(s)	738, 1121
Bse1I	ACTGG	2 cut(s)	1878, 2008
Bse3DI	GCAATG	2 cut(s)	612, 1404
BseBI	CCWGG	2 cut(s)	1215, 2057
BseDI	CCNNGG	4 cut(s)	369, 522, 1167, 1708
BseGI	GGATG	6 cut(s)	1194, 1303, 1669, 2022, 2065, 2593
BseLI	CCNNNNNNNGG	3 cut(s)	881, 1317, 2450
BseMI	GCAATG	2 cut(s)	612, 1404
BseNI	ACTGG	2 cut(s)	1878, 2008
BseRI	GAGGAG	1 cut(s)	1007
BseX3I	CGGCCG	1 cut(s)	406
BsgI	GTGCAG	3 cut(s)	503, 2028, 2628
Bsh1236I	CGCG	3 cut(s)	243, 1415, 1983
Bsh1285I	CGRYCG	1 cut(s)	409
BshFI	GGCC	6 cut(s)	126, 408, 757, 2257, 2300, 2443
BshNI	GGYRCC	1 cut(s)	735
BsiEI	CGRYCG	1 cut(s)	409
BsiHKCI	CYCGRG	1 cut(s)	1958
BsiSI	CCGG	6 cut(s)	405, 733, 739, 1122, 1230, 1334
BslFI	GGGAC	1 cut(s)	1246
BslI	CCNNNNNNNGG	3 cut(s)	881, 1317, 2450
BsmBI	CGTCTC	1 cut(s)	850
BsmFI	GGGAC	1 cut(s)	1246
BsnI	GGCC	6 cut(s)	126, 408, 757, 2257, 2300, 2443
Bso31I	GGTCTC	3 cut(s)	1180, 1432, 1902
BsoBI	CYCGRG	1 cut(s)	1958
Bsp1407I	TGTACA	2 cut(s)	381, 1071
Bsp143I	GATC	8 cut(s)	360, 833, 912, 1011, 1105, 1544, 1677, 2167
BspANI	GGCC	6 cut(s)	126, 408, 757, 2257, 2300, 2443
BspFNI	CGCG	3 cut(s)	243, 1415, 1983
BspHI	TCATGA	1 cut(s)	1237
BspLI	GGNNCC	6 cut(s)	737, 801, 855, 1332, 1754, 1900
BspMAI	CTGCAG	1 cut(s)	2410
BspMI	ACCTGC	4 cut(s)	1608, 1938, 2278, 2369
BspOI	GCTAGC	1 cut(s)	1133
BspPI	GGATC	4 cut(s)	368, 841, 920, 2162
BspT107I	GGYRCC	1 cut(s)	735
BspTNI	GGTCTC	3 cut(s)	1180, 1432, 1902
BsrDI	GCAATG	2 cut(s)	612, 1404
BsrFI	RCCGGY	2 cut(s)	738, 1121
BsrGI	TGTACA	2 cut(s)	381, 1071
BsrI	ACTGG	2 cut(s)	1878, 2008
BssAI	RCCGGY	2 cut(s)	738, 1121
BssECI	CCNNGG	4 cut(s)	369, 522, 1167, 1708
BssMI	GATC	8 cut(s)	360, 833, 912, 1011, 1105, 1544, 1677, 2167
BssT1I	CCWWGG	1 cut(s)	1708
Bst2UI	CCWGG	2 cut(s)	1215, 2057
Bst4CI	ACNGT	3 cut(s)	188, 881, 2097
Bst6I	CTCTTC	5 cut(s)	80, 319, 687, 1117, 1606
BstAPI	GCANNNNNTGC	3 cut(s)	818, 1996, 2249
BstAUI	TGTACA	2 cut(s)	381, 1071
BstC8I	GCNNGC	8 cut(s)	710, 1127, 1131, 1737, 1981, 1988, 2365, 2560
BstDSI	CCRYGG	1 cut(s)	1167
BstF5I	GGATG	6 cut(s)	1194, 1303, 1669, 2022, 2065, 2593
BstFNI	CGCG	3 cut(s)	243, 1415, 1983
BstH2I	RGCGCY	2 cut(s)	195, 1977
BstHHI	GCGC	4 cut(s)	194, 904, 1415, 1976
BstKTI	GATC	8 cut(s)	363, 836, 915, 1014, 1108, 1547, 1680, 2170
BstMBI	GATC	8 cut(s)	360, 833, 912, 1011, 1105, 1544, 1677, 2167
BstMCI	CGRYCG	1 cut(s)	409
BstNI	CCWGG	2 cut(s)	1215, 2057
BstNSI	RCATGY	5 cut(s)	35, 937, 1804, 1891, 2132
BstSCI	CCNGG	4 cut(s)	403, 1213, 1229, 2055
BstSFI	CTRYAG	2 cut(s)	2406, 2625
BstUI	CGCG	3 cut(s)	243, 1415, 1983
BstV2I	GAAGAC	1 cut(s)	2529
BstX2I	RGATCY	3 cut(s)	360, 833, 912
BstXI	CCANNNNNNTGG	1 cut(s)	1919
BstYI	RGATCY	3 cut(s)	360, 833, 912
BstZI	CGGCCG	1 cut(s)	406
BsuI	GTATCC	1 cut(s)	882
BsuRI	GGCC	6 cut(s)	126, 408, 757, 2257, 2300, 2443
BtgI	CCRYGG	1 cut(s)	1167
BtgZI	GCGATG	1 cut(s)	1805
BtsCI	GGATG	6 cut(s)	1194, 1303, 1669, 2022, 2065, 2593
BtsI	GCAGTG	3 cut(s)	1134, 2321, 2616
BtsIMutI	CAGTG	6 cut(s)	855, 886, 1134, 1835, 2321, 2616
BveI	ACCTGC	4 cut(s)	1608, 1938, 2278, 2369
Cac8I	GCNNGC	8 cut(s)	710, 1127, 1131, 1737, 1981, 1988, 2365, 2560
CaiI	CAGNNNCTG	1 cut(s)	809
CciI	TCATGA	1 cut(s)	1237
CfoI	GCGC	4 cut(s)	194, 904, 1415, 1976
Cfr10I	RCCGGY	2 cut(s)	738, 1121
Cfr13I	GGNCC	5 cut(s)	238, 755, 1211, 1331, 1898
CpoI	CGGWCCG	1 cut(s)	238
CseI	GACGC	2 cut(s)	476, 662
CspI	CGGWCCG	1 cut(s)	238
DpnI	GATC	8 cut(s)	362, 835, 914, 1013, 1107, 1546, 1679, 2169
DpnII	GATC	8 cut(s)	360, 833, 912, 1011, 1105, 1544, 1677, 2167
DriI	GACNNNNNGTC	1 cut(s)	259
EaeI	YGGCCR	2 cut(s)	406, 2298
EagI	CGGCCG	1 cut(s)	406
Eam1104I	CTCTTC	5 cut(s)	80, 319, 687, 1117, 1606
Eam1105I	GACNNNNNGTC	1 cut(s)	259
EarI	CTCTTC	5 cut(s)	80, 319, 687, 1117, 1606
EclXI	CGGCCG	1 cut(s)	406
Eco130I	CCWWGG	1 cut(s)	1708
Eco147I	AGGCCT	2 cut(s)	2257, 2443
Eco31I	GGTCTC	3 cut(s)	1180, 1432, 1902
Eco47I	GGWCC	4 cut(s)	238, 1211, 1331, 1898
Eco52I	CGGCCG	1 cut(s)	406
Eco57I	CTGAAG	3 cut(s)	270, 2164, 2408
Eco88I	CYCGRG	1 cut(s)	1958
EcoRII	CCWGG	2 cut(s)	1213, 2055
EcoT14I	CCWWGG	1 cut(s)	1708
EcoT22I	ATGCAT	1 cut(s)	823
ErhI	CCWWGG	1 cut(s)	1708
Esp3I	CGTCTC	1 cut(s)	850
FalI	AAGNNNNNCTT	4 cut(s)	540, 572, 1692, 1724
FaqI	GGGAC	1 cut(s)	1246
FauI	CCCGC	3 cut(s)	450, 1118, 1360
FbaI	TGATCA	1 cut(s)	1011
FblI	GTMKAC	2 cut(s)	2214, 2349
FokI	GGATG	6 cut(s)	1181, 1310, 1676, 2009, 2072, 2600
FspBI	CTAG	4 cut(s)	1130, 1506, 1539, 2445
GlaI	GCGC	4 cut(s)	193, 903, 1414, 1975
HaeII	RGCGCY	2 cut(s)	195, 1977
HaeIII	GGCC	6 cut(s)	126, 408, 757, 2257, 2300, 2443
HapII	CCGG	6 cut(s)	405, 733, 739, 1122, 1230, 1334
HgaI	GACGC	2 cut(s)	476, 662
HhaI	GCGC	4 cut(s)	194, 904, 1415, 1976
Hin6I	GCGC	4 cut(s)	192, 902, 1413, 1974
HinP1I	GCGC	4 cut(s)	192, 902, 1413, 1974
HincII	GTYRAC	1 cut(s)	153
HindII	GTYRAC	1 cut(s)	153
HindIII	AAGCTT	2 cut(s)	1291, 1733
HpaII	CCGG	6 cut(s)	405, 733, 739, 1122, 1230, 1334
HphI	GGTGA	5 cut(s)	85, 1111, 1348, 1687, 2302
Hpy99I	CGWCG	2 cut(s)	470, 2047
HpyAV	CCTTC	8 cut(s)	64, 71, 80, 174, 1663, 1913, 2140, 2178
HpyCH4III	ACNGT	3 cut(s)	188, 881, 2097
HpyCH4IV	ACGT	4 cut(s)	149, 227, 747, 843
HpySE526I	ACGT	4 cut(s)	149, 227, 747, 843
HspAI	GCGC	4 cut(s)	192, 902, 1413, 1974
KpnI	GGTACC	1 cut(s)	739
Ksp22I	TGATCA	1 cut(s)	1011
Kzo9I	GATC	8 cut(s)	360, 833, 912, 1011, 1105, 1544, 1677, 2167
LmnI	GCTCC	8 cut(s)	199, 216, 652, 799, 859, 884, 1488, 2113
LweI	GCATC	4 cut(s)	32, 454, 2028, 2503
MaeI	CTAG	4 cut(s)	1130, 1506, 1539, 2445
MaeII	ACGT	4 cut(s)	149, 227, 747, 843
MaeIII	GTNAC	7 cut(s)	10, 1017, 1028, 1390, 1499, 1638, 2629
MalI	GATC	8 cut(s)	362, 835, 914, 1013, 1107, 1546, 1679, 2169
MboI	GATC	8 cut(s)	360, 833, 912, 1011, 1105, 1544, 1677, 2167
MflI	RGATCY	3 cut(s)	360, 833, 912
MluCI	AATT	7 cut(s)	139, 172, 907, 961, 1162, 1657, 2065
MlyI	GAGTC	4 cut(s)	287, 662, 1228, 2545
MmeI	TCCRAC	7 cut(s)	489, 717, 730, 956, 1122, 1698, 2097
Mph1103I	ATGCAT	1 cut(s)	823
MseI	TTAA	7 cut(s)	279, 396, 947, 1050, 1184, 1251, 1776
MslI	CAYNNNNRTG	7 cut(s)	539, 1026, 1917, 1942, 2004, 2031, 2141
MspA1I	CMGCKG	5 cut(s)	251, 290, 1367, 2141, 2636
MspI	CCGG	6 cut(s)	405, 733, 739, 1122, 1230, 1334
MspR9I	CCNGG	4 cut(s)	405, 1215, 1231, 2057
MvaI	CCWGG	2 cut(s)	1215, 2057
MvnI	CGCG	3 cut(s)	243, 1415, 1983
NciI	CCSGG	2 cut(s)	405, 1231
NdeII	GATC	8 cut(s)	360, 833, 912, 1011, 1105, 1544, 1677, 2167
NheI	GCTAGC	1 cut(s)	1129
NlaIV	GGNNCC	6 cut(s)	737, 801, 855, 1332, 1754, 1900
NmeAIII	GCCGAG	1 cut(s)	2326
NmuCI	GTSAC	4 cut(s)	10, 1028, 1390, 2629
NsiI	ATGCAT	1 cut(s)	823
NspI	RCATGY	5 cut(s)	35, 937, 1804, 1891, 2132
OliI	CACNNNNGTG	2 cut(s)	539, 2141
PaeR7I	CTCGAG	1 cut(s)	1958
PagI	TCATGA	1 cut(s)	1237
PaqCI	CACCTGC	1 cut(s)	2278
PceI	AGGCCT	2 cut(s)	2257, 2443
PciI	ACATGT	2 cut(s)	31, 1887
PfeI	GAWTC	6 cut(s)	332, 374, 418, 579, 941, 2621
PfoI	TCCNGGA	2 cut(s)	1229, 2055
PleI	GAGTC	4 cut(s)	287, 662, 1228, 2544
PpsI	GAGTC	4 cut(s)	287, 662, 1228, 2544
PscI	ACATGT	2 cut(s)	31, 1887
PshBI	ATTAAT	1 cut(s)	1776
Psp1406I	AACGTT	1 cut(s)	747
Psp6I	CCWGG	2 cut(s)	1213, 2055
PspGI	CCWGG	2 cut(s)	1213, 2055
PspN4I	GGNNCC	6 cut(s)	737, 801, 855, 1332, 1754, 1900
PspPI	GGNCC	5 cut(s)	238, 755, 1211, 1331, 1898
PspXI	VCTCGAGB	1 cut(s)	1958
PstI	CTGCAG	1 cut(s)	2410
PstNI	CAGNNNCTG	1 cut(s)	809
PsuI	RGATCY	3 cut(s)	360, 833, 912
PvuII	CAGCTG	4 cut(s)	251, 290, 2141, 2636
RseI	CAYNNNNRTG	7 cut(s)	539, 1026, 1917, 1942, 2004, 2031, 2141
Rsr2I	CGGWCCG	1 cut(s)	238
RsrII	CGGWCCG	1 cut(s)	238
SaqAI	TTAA	7 cut(s)	279, 396, 947, 1050, 1184, 1251, 1776
Sau3AI	GATC	8 cut(s)	360, 833, 912, 1011, 1105, 1544, 1677, 2167
Sau96I	GGNCC	5 cut(s)	238, 755, 1211, 1331, 1898
ScaI	AGTACT	1 cut(s)	2177
SchI	GAGTC	4 cut(s)	287, 662, 1228, 2545
ScrFI	CCNGG	4 cut(s)	405, 1215, 1231, 2057
SfaNI	GCATC	4 cut(s)	32, 454, 2028, 2503
SfcI	CTRYAG	2 cut(s)	2406, 2625
Sfr274I	CTCGAG	1 cut(s)	1958
SgrAI	CRCCGGYG	1 cut(s)	1121
SinI	GGWCC	4 cut(s)	238, 1211, 1331, 1898
SlaI	CTCGAG	1 cut(s)	1958
SmiMI	CAYNNNNRTG	7 cut(s)	539, 1026, 1917, 1942, 2004, 2031, 2141
SmlI	CTYRAG	2 cut(s)	1443, 1958
SmoI	CTYRAG	2 cut(s)	1443, 1958
Sse9I	AATT	7 cut(s)	139, 172, 907, 961, 1162, 1657, 2065
SseBI	AGGCCT	2 cut(s)	2257, 2443
SspMI	CTAG	4 cut(s)	1130, 1506, 1539, 2445
StuI	AGGCCT	2 cut(s)	2257, 2443
StyD4I	CCNGG	4 cut(s)	403, 1213, 1229, 2055
StyI	CCWWGG	1 cut(s)	1708
TaaI	ACNGT	3 cut(s)	188, 881, 2097
TaiI	ACGT	4 cut(s)	152, 230, 750, 846
TaqI	TCGA	8 cut(s)	183, 1906, 1925, 1959, 2099, 2238, 2436, 2534
TasI	AATT	7 cut(s)	139, 172, 907, 961, 1162, 1657, 2065
TatI	WGTACW	5 cut(s)	381, 1059, 1071, 1884, 2175
TauI	GCSGC	2 cut(s)	411, 1986
TfiI	GAWTC	6 cut(s)	332, 374, 418, 579, 941, 2621
Tru1I	TTAA	7 cut(s)	279, 396, 947, 1050, 1184, 1251, 1776
Tru9I	TTAA	7 cut(s)	279, 396, 947, 1050, 1184, 1251, 1776
TscAI	CASTG	6 cut(s)	855, 886, 1141, 1842, 2321, 2616
TseFI	GTSAC	4 cut(s)	10, 1028, 1390, 2629
Tsp45I	GTSAC	4 cut(s)	10, 1028, 1390, 2629
TspDTI	ATGAA	3 cut(s)	306, 1926, 2675
TspRI	CASTG	6 cut(s)	855, 886, 1141, 1842, 2321, 2616
VpaK11BI	GGWCC	4 cut(s)	238, 1211, 1331, 1898
VspI	ATTAAT	1 cut(s)	1776
XapI	RAATTY	3 cut(s)	139, 961, 2065
XbaI	TCTAGA	2 cut(s)	1505, 1538
XceI	RCATGY	5 cut(s)	35, 937, 1804, 1891, 2132
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1205
XhoI	CTCGAG	1 cut(s)	1958
XmiI	GTMKAC	2 cut(s)	2214, 2349
XspI	CTAG	4 cut(s)	1130, 1506, 1539, 2445
ZrmI	AGTACT	1 cut(s)	2177
Zsp2I	ATGCAT	1 cut(s)	823

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.