FvH4_5g16070

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0018443
Physical Location & Seq
Reverse (-)
9143155 .. 9150164
7010 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16070.t1

Sequence Viewer

Length: 2946 bp
ATGGCCACCAGCAGCAGAAGCGGTGTACCATTTCCTTCGTCCTCGGAACCACCATGGAAGTACGATGTCTTCTTGAGCTTTAGAGGTTCAGATACACGCCGCAACTTCACAGATCATCTCTACATGGCATTGGAACAAGCTGGAGTCCGCGTCTTTCGTGATGATGAGGAACTAATGAGAGGGGAATCAACGGCAGTTGCTCTGCTGACAGCAATAGTAGAGTCGAAGATCTCTATCGTCGTCTTCTCCAAAAATTATGCAAGTTCCAGTTGGTGTCTGGACGAGCTGGTAAAGATCTTGGAGTGTAAATCAATGTATGGACAAATGGTGACTCCAGTATTCTATGACGTTAAACCTTCAGATGTGAAATTCCAGAGGGATAGTTTTGCATACGCATTTGACAAACATGAAAAGCGCTACGATATGGATAAGGTGCTGATGTGGAGAGATGCTCTAACTTCTGTTGCAAATATAACGGGCTGGGATGTTTCAAACTACGGGTTTGAATCAGAAGTCATCAGGAACATTGTAGTGACAATTACTGAACTGTTGGAGAACCCAGAAGTGGATGTTGAAGACTTGCCGGAAATGGATGATGGACAGTTGGTGGACCCAGAAGTGACTGTAGCCAAGTTTCATGTTGGAATTCAGTCTCGGGTGGAAGATTTGGCTAGTCATCTAGATGTTGGGTCAGATGAGGTACAAATAGTTGGAATTTTCGGGGTGCAGGGAATTGGCAAAACGACGGTTGCCAAAGCTATGTATGAACGATATCGTAATAGCTTTGACAGTGCATGTTTCCTTGTCAATGTGAGGGACACTATAAAAGAACCGAATGGTCTGATTAGTTTGCAGGAACAACTTCATGATCTCTTGGAAACAAAGATAGACGTAGGAGTTGTTTCTGCAGGGTTTCAACGGATCAAAAGAGAAAGAAGGGTACTACTCATAATCGATGACATAGATGATAAAAAGCAACTAGAACAATTGGCCATTGAACGTGCTAGGTTTGGTTCTGGAAGTAGAATTGTCATAATAGCAAGAGAGGAAGCTCTGCTGAAGCAACTTAAAGTTGATACAACATATTCACCACGACCAATGAACAAAGAGGAAGCGATTGAGCTCTTTAGTTGGCATGCCTTTGGGAAGTGTTCTCCTAATGAAAAATATTCTGAACACTCAAAAACTGTTGTTGCTTTTTTGGGGGGTTTGCCACTACTTCTTACAGTCATAGGTCATGATCTTTATGGAAAAGGCATGAAAAGGTGGCAACTGTTGATGAAATGTATTGCAAACGAGGAAGCTAGAATTTTGAAAGACCTATTTGTGTATTGTTATTCTCTCTTTCCAACAGGTTATGAATTCAGGAAAGATGAGTTGGTTCAGTTGTGGATAGCTGAAGGCTTTATAGTGGACAAGTATGCAGAGAAGATGGAGGATACTGGCATTTCGTATTTCAATTACTTCGAGAATAAAGGTTATTTTTTGCCCTCAAGATGTAATTTTGGAGTGGATTTTGATTCGGTCTCCTCGGTGACTTGCAATTCCAACAACCTGTACAAGATAAATACTGAAAAGGCTGATGTTTCAAAAAAAAAAACTTCTCGGGATCATTATTCCAGAGCAGTGGATCCGGTTTCTATCTCACGACACACAGTTTCTCAGGCTGAGGATGGATTAAAATTGGTGGATGAGCCTTCTATTGCAGAAAACAAAATTTCTATGGTTGATAGTTATTTTGGAGCCCTGGAAGTGGTTTCTGTTTCAGAAAACACACTTTCCCGGGCTAATGATTTGAGAGCCAGGGAAATGGTTTCAGGGTACACAGCTTCTGAGCTTGATTATTTTAGAGCTGCAGATGCAAATCTGTATGGAACCTCTGCAACTACAACACGACATTTGTCTTTGACGTTTAAAGACATCGATGATATGAGTTTTGAAAAACTGAAGGAACTGAAGGAACTGCGCACACTGCTTTTTCTCTCGGATAGTGGATCTTCCATTACGCATGTTCCTCGTAATTTCTTCTGGAGCTTAAAGTTCTTGAGAACGTTGAAGTTGCGTGGAACACTCATATCTGAACTGCCAAGCTCCATTCGGAATGTCAAAGAATTACGATATCTTGATGTCTCTTACACACCCATTACACAGTTGCCTGAATCAATAACTTACCTTCATAATTTACAAACCTTAAAACTTAGAGGTTGTGTACAATTTTATCAGTTACCTAAGGAACTGGAGCAGCTAACCAAGCTGCGCCATATTGAACTTGATATCATGAGGCAACTGGATTCCTTGCCTGCAAACTTGGGAAAGTTGACTCACCTGCAGACACTGTCAGCATTTCTTGTTGATAAAGATGATGGGTGCTGTATCGGACAGTTGAAGAATTTGAATGACCTCAAAGGAGCACTCTGCATTTCAAGGCTTGAAAACGTGATGAAAGTGGAAGAGGCCGAGGAAGCTTCTTTGGTTCAGAAGAAATGTCTCGAAACGTTGGAGCTCCGGTGGAGTCATCTGTTATCTACTAATAAAGAGAAGCAAGAAGAAATCCTTGAATGGCTGGAACCCCATTATGGCCTTAAAGAGTTACAAATACACCACTATGGTGGGTCAAAACTTCCAACTTGGATTAGTAATCCATCTTTTGCTGACCTTGTTGCTGTTACCCTCTATAGATGCAACAATTGTGTACTTCTCCCTTCCATGGGAAATTTACCAGCACTAAAAGTGCTTTCCATTATGGAGATGAATGAGGTGACAGAAATCAATCATCAATTTTTCAGAAATGGCCTAGCTGATGATCAAGCTTTCTCTGCATTTCCAAAACTTGAGACACTAGAAATTGACATCATGGTCAATCTGAAAGATTGGCAGGAGGTTCAGAAAGGAGTTGTCCAAAGCTTCAGCCCAGTGATCAACTACCAGATTCCCTTGAAGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

982

Amino Acids

111.73

Weight (kDa)

5.44

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 184 2.1e-50 TIR domain
TIR_2 PF13676 23 - 126 2.7e-15 TIR domain
NB-ARC PF00931 217 - 381 4.2e-16 NB-ARC domain
NACHT PF05729 237 - 376 2.1e-06 NACHT domain
WHD_DRP PF23559 448 - 493 1.5e-12 Disease resistance protein Winged helix domain
LRR_14 PF23598 640 - 705 5.3e-06 Leucine-rich repeat region
LRR_14 PF23598 699 - 950 5e-28 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 792 - 917 6.9e-38 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018443)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g16070 FvH4_5g16070 FvH4_5g16070 FvH4_5g16070 FvH4_5g16070
rosa_rugosa Rorug07G0132500 Rorug07G0132600
rosa_samantha Rh7AG265500 Rh7CG282200
rosa_wichuraiana Rw7G022730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2334
AasI GACNNNNNNGTC 1 cut(s) 2855
Acc16I TGCGCA 1 cut(s) 1967
Acc36I ACCTGC 1 cut(s) 2334
AccII CGCG 1 cut(s) 150
AciI CCGC 3 cut(s) 21, 100, 148
AclI AACGTT 2 cut(s) 2051, 2495
AclWI GGATC 5 cut(s) 929, 1617, 1625, 1638, 2002
AcoI YGGCCR 2 cut(s) 3, 990
AcsI RAATTY 8 cut(s) 368, 645, 714, 1308, 1361, 1716, 2389, 2713
AcuI CTGAAG 6 cut(s) 342, 1079, 1419, 1967, 1976, 2890
AfaI GTAC 8 cut(s) 27, 62, 702, 942, 1559, 1823, 2211, 2694
AfeI AGCGCT 1 cut(s) 416
AfiI CCNNNNNNNGG 5 cut(s) 565, 1753, 2576, 2707, 2708
AjnI CCWGG 2 cut(s) 1746, 1802
AjuI GAANNNNNNNTTGG 2 cut(s) 1361, 1393
AleI CACNNNNGTG 1 cut(s) 2607
Alw21I GWGCWC 3 cut(s) 1125, 2413, 2505
Alw26I GTCTC 5 cut(s) 657, 1531, 2134, 2492, 2828
AlwI GGATC 5 cut(s) 929, 1617, 1625, 1638, 2002
AlwNI CAGNNNCTG 2 cut(s) 1832, 2335
Ama87I CYCGRG 3 cut(s) 654, 1605, 1782
Aor51HI AGCGCT 1 cut(s) 416
AoxI GGCC 5 cut(s) 3, 990, 2454, 2578, 2791
ApeKI GCWGC 4 cut(s) 12, 1853, 2242, 2254
ApoI RAATTY 8 cut(s) 368, 645, 714, 1308, 1361, 1716, 2389, 2713
Asp700I GAANNNNTTC 1 cut(s) 861
AspLEI GCGC 3 cut(s) 417, 1968, 2259
AspS9I GGNCC 1 cut(s) 610
AsuC2I CCSGG 2 cut(s) 1783, 1784
AsuHPI GGTGA 5 cut(s) 340, 1080, 1546, 2315, 2770
AvaI CYCGRG 3 cut(s) 654, 1605, 1782
AvaII GGWCC 1 cut(s) 610
AxyI CCTNAGG 1 cut(s) 2229
BalI TGGCCA 2 cut(s) 5, 992
BamHI GGATCC 1 cut(s) 1630
BanII GRGCYC 3 cut(s) 1125, 1747, 2505
BarI GAAGNNNNNNTAC 2 cut(s) 53, 85
BbsI GAAGAC 3 cut(s) 61, 235, 582
Bbv12I GWGCWC 3 cut(s) 1125, 2413, 2505
BbvCI CCTCAGC 1 cut(s) 1668
BbvI GCAGC 4 cut(s) 24, 1840, 2241, 2254
BccI CCATC 5 cut(s) 590, 1426, 1667, 2357, 2650
BceAI ACGGC 1 cut(s) 207
BcgI CGANNNNNNTGC 2 cut(s) 1997, 2031
BciT130I CCWGG 2 cut(s) 1748, 1804
BciVI GTATCC 1 cut(s) 1432
BclI TGATCA 2 cut(s) 2803, 2916
BcnI CCSGG 2 cut(s) 1783, 1784
BcoDI GTCTC 5 cut(s) 657, 1531, 2134, 2492, 2828
BfaI CTAG 7 cut(s) 672, 680, 980, 1005, 1305, 2795, 2840
BfmI CTRYAG 5 cut(s) 624, 906, 1854, 2327, 2674
BfoI RGCGCY 1 cut(s) 418
BfuAI ACCTGC 1 cut(s) 2334
BfuI GTATCC 1 cut(s) 1432
BglII AGATCT 2 cut(s) 228, 294
BisI GCNGC 5 cut(s) 13, 100, 1854, 2243, 2255
BlsI GCNGC 5 cut(s) 14, 101, 1855, 2244, 2256
Bme1390I CCNGG 4 cut(s) 1748, 1783, 1784, 1804
Bme18I GGWCC 1 cut(s) 610
BmeT110I CYCGRG 3 cut(s) 654, 1605, 1782
BmgT120I GGNCC 1 cut(s) 610
BmiI GGNNCC 6 cut(s) 48, 612, 1632, 1744, 1876, 2568
BmrFI CCNGG 4 cut(s) 1748, 1783, 1784, 1804
BmrI ACTGGG 1 cut(s) 2906
BmsI GCATC 3 cut(s) 439, 1849, 2669
BmuI ACTGGG 1 cut(s) 2906
BoxI GACNNNNGTC 1 cut(s) 1900
BpiI GAAGAC 3 cut(s) 61, 235, 582
BplI GAGNNNNNCTC 2 cut(s) 436, 468
BpmI CTGGAG 4 cut(s) 162, 318, 2050, 2258
Bpu10I CCTNAGC 1 cut(s) 1668
BpuEI CTTGAG 4 cut(s) 94, 1477, 2065, 2852
BpuMI CCSGG 2 cut(s) 1783, 1784
Bsa29I ATCGAT 2 cut(s) 954, 1923
BsaBI GATNNNNATC 2 cut(s) 233, 1863
BsaI GGTCTC 1 cut(s) 1531
BsaJI CCNNGG 8 cut(s) 42, 53, 1530, 1746, 1782, 1803, 2457, 2706
BsaWI WCCGGW 2 cut(s) 1633, 2505
BsaXI ACNNNNNCTCC 4 cut(s) 135, 165, 2492, 2522
Bsc4I CCNNNNNNNGG 5 cut(s) 565, 1753, 2576, 2707, 2708
Bse1I ACTGG 6 cut(s) 267, 335, 1447, 2241, 2292, 2912
Bse21I CCTNAGG 1 cut(s) 2229
Bse8I GATNNNNATC 2 cut(s) 233, 1863
BseBI CCWGG 2 cut(s) 1748, 1804
BseCI ATCGAT 2 cut(s) 954, 1923
BseDI CCNNGG 8 cut(s) 42, 53, 1530, 1746, 1782, 1803, 2457, 2706
BseGI GGATG 5 cut(s) 490, 574, 598, 1678, 1696
BseJI GATNNNNATC 2 cut(s) 233, 1863
BseLI CCNNNNNNNGG 5 cut(s) 565, 1753, 2576, 2707, 2708
BseMII CTCAG 3 cut(s) 1659, 1676, 1824
BseNI ACTGG 6 cut(s) 267, 335, 1447, 2241, 2292, 2912
BseRI GAGGAG 1 cut(s) 1519
BseXI GCAGC 4 cut(s) 24, 1840, 2241, 2254
BseYI CCCAGC 1 cut(s) 480
BsgI GTGCAG 1 cut(s) 746
Bsh1236I CGCG 1 cut(s) 150
BshFI GGCC 5 cut(s) 5, 992, 2456, 2580, 2793
BshVI ATCGAT 2 cut(s) 954, 1923
BsiHKAI GWGCWC 3 cut(s) 1125, 2413, 2505
BsiHKCI CYCGRG 3 cut(s) 654, 1605, 1782
BsiSI CCGG 4 cut(s) 584, 1634, 1783, 2506
BslFI GGGAC 1 cut(s) 830
BslI CCNNNNNNNGG 5 cut(s) 565, 1753, 2576, 2707, 2708
BsmAI GTCTC 5 cut(s) 657, 1531, 2134, 2492, 2828
BsmFI GGGAC 1 cut(s) 830
BsnI GGCC 5 cut(s) 5, 992, 2456, 2580, 2793
Bso31I GGTCTC 1 cut(s) 1531
BsoBI CYCGRG 3 cut(s) 654, 1605, 1782
Bsp1286I GDGCHC 4 cut(s) 1125, 1747, 2413, 2505
Bsp1407I TGTACA 2 cut(s) 1557, 2209
Bsp19I CCATGG 2 cut(s) 53, 2706
BspACI CCGC 3 cut(s) 21, 100, 148
BspANI GGCC 5 cut(s) 5, 992, 2456, 2580, 2793
BspCNI CTCAG 3 cut(s) 1660, 1675, 1825
BspDI ATCGAT 2 cut(s) 954, 1923
BspFNI CGCG 1 cut(s) 150
BspHI TCATGA 3 cut(s) 865, 1237, 2277
BspLI GGNNCC 6 cut(s) 48, 612, 1632, 1744, 1876, 2568
BspMAI CTGCAG 3 cut(s) 910, 1858, 2331
BspMI ACCTGC 1 cut(s) 2334
BspPI GGATC 5 cut(s) 929, 1617, 1625, 1638, 2002
BspTNI GGTCTC 1 cut(s) 1531
BsrGI TGTACA 2 cut(s) 1557, 2209
BsrI ACTGG 6 cut(s) 267, 335, 1447, 2241, 2292, 2912
BssECI CCNNGG 8 cut(s) 42, 53, 1530, 1746, 1782, 1803, 2457, 2706
BssT1I CCWWGG 2 cut(s) 53, 2706
Bst2UI CCWGG 2 cut(s) 1748, 1804
Bst6I CTCTTC 1 cut(s) 2445
BstAUI TGTACA 2 cut(s) 1557, 2209
BstC8I GCNNGC 2 cut(s) 1137, 2301
BstDEI CTNAG 5 cut(s) 1662, 1668, 1833, 2198, 2229
BstDSI CCRYGG 2 cut(s) 53, 2706
BstF5I GGATG 5 cut(s) 490, 574, 598, 1678, 1696
BstFNI CGCG 1 cut(s) 150
BstH2I RGCGCY 1 cut(s) 418
BstHHI GCGC 3 cut(s) 417, 1968, 2259
BstMAI GTCTC 5 cut(s) 657, 1531, 2134, 2492, 2828
BstMWI GCNNNNNNNGC 6 cut(s) 18, 1859, 1972, 2251, 2462, 2816
BstNI CCWGG 2 cut(s) 1748, 1804
BstNSI RCATGY 3 cut(s) 798, 1139, 2012
BstPAI GACNNNNGTC 1 cut(s) 1900
BstSCI CCNGG 4 cut(s) 1746, 1781, 1782, 1802
BstSFI CTRYAG 5 cut(s) 624, 906, 1854, 2327, 2674
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 4 cut(s) 24, 1840, 2241, 2254
BstV2I GAAGAC 3 cut(s) 61, 235, 582
BstX2I RGATCY 4 cut(s) 228, 294, 1630, 1994
BstXI CCANNNNNNTGG 3 cut(s) 1627, 1810, 2609
BstYI RGATCY 4 cut(s) 228, 294, 1630, 1994
Bsu15I ATCGAT 2 cut(s) 954, 1923
Bsu36I CCTNAGG 1 cut(s) 2229
BsuI GTATCC 1 cut(s) 1432
BsuRI GGCC 5 cut(s) 5, 992, 2456, 2580, 2793
BsuTUI ATCGAT 2 cut(s) 954, 1923
BtgI CCRYGG 2 cut(s) 53, 2706
BtsCI GGATG 5 cut(s) 490, 574, 598, 1678, 1696
BtsI GCAGTG 2 cut(s) 1632, 1970
BtsIMutI CAGTG 5 cut(s) 796, 1632, 1970, 2333, 2919
BveI ACCTGC 1 cut(s) 2334
Cac8I GCNNGC 2 cut(s) 1137, 2301
CaiI CAGNNNCTG 2 cut(s) 1832, 2335
CciI TCATGA 3 cut(s) 865, 1237, 2277
CfoI GCGC 3 cut(s) 417, 1968, 2259
Cfr13I GGNCC 1 cut(s) 610
Cfr9I CCCGGG 1 cut(s) 1782
ClaI ATCGAT 2 cut(s) 954, 1923
CseI GACGC 1 cut(s) 139
Csp6I GTAC 8 cut(s) 26, 61, 701, 941, 1558, 1822, 2210, 2693
CviQI GTAC 8 cut(s) 26, 61, 701, 941, 1558, 1822, 2210, 2693
DdeI CTNAG 5 cut(s) 1662, 1668, 1833, 2198, 2229
DraI TTTAAA 1 cut(s) 1915
DrdI GACNNNNNNGTC 1 cut(s) 2855
DseDI GACNNNNNNGTC 1 cut(s) 2855
EaeI YGGCCR 2 cut(s) 3, 990
Eam1104I CTCTTC 1 cut(s) 2445
EarI CTCTTC 1 cut(s) 2445
Ecl136II GAGCTC 2 cut(s) 1123, 2503
Eco130I CCWWGG 2 cut(s) 53, 2706
Eco24I GRGCYC 3 cut(s) 1125, 1747, 2505
Eco31I GGTCTC 1 cut(s) 1531
Eco32I GATATC 3 cut(s) 773, 2120, 2275
Eco47I GGWCC 1 cut(s) 610
Eco47III AGCGCT 1 cut(s) 416
Eco53kI GAGCTC 2 cut(s) 1123, 2503
Eco57I CTGAAG 6 cut(s) 342, 1079, 1419, 1967, 1976, 2890
Eco81I CCTNAGG 1 cut(s) 2229
Eco88I CYCGRG 3 cut(s) 654, 1605, 1782
EcoICRI GAGCTC 2 cut(s) 1123, 2503
EcoRI GAATTC 2 cut(s) 645, 1361
EcoRII CCWGG 2 cut(s) 1746, 1802
EcoRV GATATC 3 cut(s) 773, 2120, 2275
EcoT14I CCWWGG 2 cut(s) 53, 2706
EcoT38I GRGCYC 3 cut(s) 1125, 1747, 2505
ErhI CCWWGG 2 cut(s) 53, 2706
FalI AAGNNNNNCTT 2 cut(s) 2538, 2570
FaqI GGGAC 1 cut(s) 830
FbaI TGATCA 2 cut(s) 2803, 2916
Fnu4HI GCNGC 5 cut(s) 13, 100, 1854, 2243, 2255
FokI GGATG 5 cut(s) 497, 581, 605, 1685, 1703
FriOI GRGCYC 3 cut(s) 1125, 1747, 2505
Fsp4HI GCNGC 5 cut(s) 13, 100, 1854, 2243, 2255
FspBI CTAG 7 cut(s) 672, 680, 980, 1005, 1305, 2795, 2840
FspI TGCGCA 1 cut(s) 1967
GlaI GCGC 3 cut(s) 416, 1967, 2258
GluI GCNGC 5 cut(s) 13, 100, 1854, 2243, 2255
GsaI CCCAGC 1 cut(s) 484
GsuI CTGGAG 4 cut(s) 162, 318, 2050, 2258
HaeII RGCGCY 1 cut(s) 418
HaeIII GGCC 5 cut(s) 5, 992, 2456, 2580, 2793
HapII CCGG 4 cut(s) 584, 1634, 1783, 2506
HgaI GACGC 1 cut(s) 139
HhaI GCGC 3 cut(s) 417, 1968, 2259
Hin6I GCGC 3 cut(s) 415, 1966, 2257
HinP1I GCGC 3 cut(s) 415, 1966, 2257
HincII GTYRAC 1 cut(s) 2319
HindII GTYRAC 1 cut(s) 2319
HindIII AAGCTT 3 cut(s) 2463, 2808, 2902
HpaII CCGG 4 cut(s) 584, 1634, 1783, 2506
HphI GGTGA 5 cut(s) 340, 1080, 1546, 2315, 2770
Hpy166II GTNNAC 7 cut(s) 26, 610, 1414, 1824, 2210, 2319, 2693
Hpy8I GTNNAC 7 cut(s) 26, 610, 1414, 1824, 2210, 2319, 2693
Hpy99I CGWCG 2 cut(s) 242, 748
HpyAV CCTTC 9 cut(s) 45, 366, 930, 1394, 1707, 1942, 1951, 2183, 2712
HpyCH4IV ACGT 7 cut(s) 348, 891, 1000, 1910, 2051, 2436, 2495
HpyF10VI GCNNNNNNNGC 6 cut(s) 18, 1859, 1972, 2251, 2462, 2816
HpyF3I CTNAG 5 cut(s) 1662, 1668, 1833, 2198, 2229
HpySE526I ACGT 7 cut(s) 348, 891, 1000, 1910, 2051, 2436, 2495
HspAI GCGC 3 cut(s) 415, 1966, 2257
Ksp22I TGATCA 2 cut(s) 2803, 2916
LmnI GCTCC 7 cut(s) 1742, 2031, 2096, 2239, 2408, 2500, 2508
Lsp1109I GCAGC 4 cut(s) 24, 1840, 2241, 2254
LweI GCATC 3 cut(s) 439, 1849, 2669
MaeI CTAG 7 cut(s) 672, 680, 980, 1005, 1305, 2795, 2840
MaeII ACGT 7 cut(s) 348, 891, 1000, 1910, 2051, 2436, 2495
MaeIII GTNAC 8 cut(s) 328, 532, 619, 1534, 2223, 2589, 2665, 2758
MfeI CAATTG 2 cut(s) 986, 2686
MflI RGATCY 4 cut(s) 228, 294, 1630, 1994
MhlI GDGCHC 4 cut(s) 1125, 1747, 2413, 2505
MlsI TGGCCA 2 cut(s) 5, 992
MluNI TGGCCA 2 cut(s) 5, 992
MlyI GAGTC 5 cut(s) 153, 230, 325, 2314, 2521
MmeI TCCRAC 7 cut(s) 531, 622, 691, 1373, 1572, 2478, 2648
Mox20I TGGCCA 2 cut(s) 5, 992
MroXI GAANNNNTTC 1 cut(s) 861
MscI TGGCCA 2 cut(s) 5, 992
MseI TTAA 7 cut(s) 351, 1068, 1679, 1914, 2036, 2192, 2583
MslI CAYNNNNRTG 4 cut(s) 530, 681, 2604, 2607
Msp20I TGGCCA 2 cut(s) 5, 992
MspI CCGG 4 cut(s) 584, 1634, 1783, 2506
MspR9I CCNGG 4 cut(s) 1748, 1783, 1784, 1804
MunI CAATTG 2 cut(s) 986, 2686
MvaI CCWGG 2 cut(s) 1748, 1804
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 6 cut(s) 18, 1859, 1972, 2251, 2462, 2816
NciI CCSGG 2 cut(s) 1783, 1784
NcoI CCATGG 2 cut(s) 53, 2706
NlaIV GGNNCC 6 cut(s) 48, 612, 1632, 1744, 1876, 2568
NmeAIII GCCGAG 1 cut(s) 2482
NmuCI GTSAC 5 cut(s) 328, 532, 619, 1534, 2758
NsbI TGCGCA 1 cut(s) 1967
NspI RCATGY 3 cut(s) 798, 1139, 2012
OliI CACNNNNGTG 1 cut(s) 2607
PaeI GCATGC 1 cut(s) 1139
PagI TCATGA 3 cut(s) 865, 1237, 2277
PaqCI CACCTGC 1 cut(s) 2334
PdmI GAANNNNTTC 1 cut(s) 861
PfeI GAWTC 6 cut(s) 185, 506, 1520, 2159, 2291, 2929
PflFI GACNNNGTC 1 cut(s) 2335
PkrI GCNGC 5 cut(s) 14, 101, 1855, 2244, 2256
PleI GAGTC 5 cut(s) 152, 229, 325, 2314, 2520
PpsI GAGTC 5 cut(s) 152, 229, 325, 2314, 2520
PshAI GACNNNNGTC 1 cut(s) 1900
Psp124BI GAGCTC 2 cut(s) 1125, 2505
Psp1406I AACGTT 2 cut(s) 2051, 2495
Psp6I CCWGG 2 cut(s) 1746, 1802
PspFI CCCAGC 1 cut(s) 480
PspGI CCWGG 2 cut(s) 1746, 1802
PspN4I GGNNCC 6 cut(s) 48, 612, 1632, 1744, 1876, 2568
PspPI GGNCC 1 cut(s) 610
PstI CTGCAG 3 cut(s) 910, 1858, 2331
PstNI CAGNNNCTG 2 cut(s) 1832, 2335
PsuI RGATCY 4 cut(s) 228, 294, 1630, 1994
PsyI GACNNNGTC 1 cut(s) 2335
RsaI GTAC 8 cut(s) 27, 62, 702, 942, 1559, 1823, 2211, 2694
RsaNI GTAC 8 cut(s) 26, 61, 701, 941, 1558, 1822, 2210, 2693
RseI CAYNNNNRTG 4 cut(s) 530, 681, 2604, 2607
SacI GAGCTC 2 cut(s) 1125, 2505
SaqAI TTAA 7 cut(s) 351, 1068, 1679, 1914, 2036, 2192, 2583
SatI GCNGC 5 cut(s) 13, 100, 1854, 2243, 2255
Sau96I GGNCC 1 cut(s) 610
SchI GAGTC 5 cut(s) 153, 230, 325, 2314, 2521
ScrFI CCNGG 4 cut(s) 1748, 1783, 1784, 1804
SduI GDGCHC 4 cut(s) 1125, 1747, 2413, 2505
SfaNI GCATC 3 cut(s) 439, 1849, 2669
SfcI CTRYAG 5 cut(s) 624, 906, 1854, 2327, 2674
SinI GGWCC 1 cut(s) 610
SmaI CCCGGG 1 cut(s) 1784
SmiMI CAYNNNNRTG 4 cut(s) 530, 681, 2604, 2607
SmlI CTYRAG 4 cut(s) 73, 1492, 2044, 2831
SmoI CTYRAG 4 cut(s) 73, 1492, 2044, 2831
SphI GCATGC 1 cut(s) 1139
SsiI CCGC 3 cut(s) 21, 100, 148
SspI AATATT 1 cut(s) 1169
SspMI CTAG 7 cut(s) 672, 680, 980, 1005, 1305, 2795, 2840
SstI GAGCTC 2 cut(s) 1125, 2505
StyD4I CCNGG 4 cut(s) 1746, 1781, 1782, 1802
StyI CCWWGG 2 cut(s) 53, 2706
TaiI ACGT 7 cut(s) 351, 894, 1003, 1913, 2054, 2439, 2498
TaqI TCGA 5 cut(s) 224, 954, 1467, 1923, 2490
TaqII GACCGA 1 cut(s) 1513
TatI WGTACW 3 cut(s) 1557, 2209, 2692
TauI GCSGC 1 cut(s) 102
TfiI GAWTC 6 cut(s) 185, 506, 1520, 2159, 2291, 2929
Tru1I TTAA 7 cut(s) 351, 1068, 1679, 1914, 2036, 2192, 2583
Tru9I TTAA 7 cut(s) 351, 1068, 1679, 1914, 2036, 2192, 2583
TscAI CASTG 5 cut(s) 796, 1632, 1977, 2340, 2919
TseFI GTSAC 5 cut(s) 328, 532, 619, 1534, 2758
TseI GCWGC 4 cut(s) 12, 1853, 2242, 2254
Tsp45I GTSAC 5 cut(s) 328, 532, 619, 1534, 2758
TspGWI ACGGA 1 cut(s) 934
TspMI CCCGGG 1 cut(s) 1782
TspRI CASTG 5 cut(s) 796, 1632, 1977, 2340, 2919
Tth111I GACNNNGTC 1 cut(s) 2335
VpaK11BI GGWCC 1 cut(s) 610
XapI RAATTY 8 cut(s) 368, 645, 714, 1308, 1361, 1716, 2389, 2713
XbaI TCTAGA 1 cut(s) 679
XceI RCATGY 3 cut(s) 798, 1139, 2012
XcmI CCANNNNNNNNNTGG 1 cut(s) 274
XmaI CCCGGG 1 cut(s) 1782
XmnI GAANNNNTTC 1 cut(s) 861
XspI CTAG 7 cut(s) 672, 680, 980, 1005, 1305, 2795, 2840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.