FvH4_5g18620

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016852
Physical Location & Seq
Forward (+)
10708829 .. 10710936
2108 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18620.t1

Sequence Viewer

Length: 1875 bp
ATGTACAAACTCGTCGCCATGATGATGATGATAATTTTTCTGATCAATCCGATACTAATATCATCGTCGTTGTCGACGTCGACCTCTGAAGCATTTGATGAATTCTACTCGGAAGAAAGAGAGGCTCTAATAAAGCTGAGAGACTCAGTGAGCTCAAACTCATCCACCTTGCACTCAAATTGGACTGGTCCTCCCTGCAACTACATTAACAGCAGCAGATGGTCTGGTATTTCTTGTTCAAACTGGCACGTCGTTCATCTTGTTCTAGAAGAAGCTCAGCTCTCAGCTACTATATCACCAGCTGGCTCTCTTCCACCCACATTTCTGCAAAACCTAACTTTCCTGAGCAAACTTAGCTTTAGGAACAACACCTTCTCCGGCTCTCTTCCTAACCTATCAAGTCTCGTGCACCTTGAATATGTGGATTTCTCATACAACCGCTTCTGGGGTTCGATACCTTTCGAGTATGCTACTGATCTGCCAAACCTGGAAGTGCTAGAGCTGCAAGAGAATTCCTTAGATGGGTCGATTCCGCCTTTCGACCAACCCACACTGAGAGCTTTTAATGTTTCTCATAATCGTCTCGAGGGTCCGATTCCTAACACTGGTGTGTTACAGAAGTTCCCGAAAAGCTCGTATGATGACAATGCACATCTGTGTGGGAGTCCTCTGGGGAATTCTTGCACTCCTCCTCCTTCTCCTCATTCCCCGTCAAACGATTCGAAAGATGATCGCCTCCCACTCGCTTTGATTGTGGCTGCAGCAGCTGTGGTTCCTTTTCTCGTTATATTTGCTTTCTTATGTTACTATAAGAAAGTGCATGGCAAAGATGAAACGACGTCAACAGATCAGGCTCAGCACACAGGATGGGCAGTGGCACAAACGAAGATGCGTGATGATAATCGTTTGGATGAGAGGTTGGGGGATCCTGAAAAGAGGGTTGAGTTGGAGTTTTTCGACAAGGACTTGCCGGCCGAGGCATTTTTTGATTTGGATGACTTACTTAGGGCTTCGGCAGAAGTGATAGGCAGGGGGGAACTGGGAACTTCATATAAAGTGACACTAGAATCGGGCCCTGTCGTGGTGGTGAAGAGAATTAGAAGCATGAACGAGTTGAGCAGGAAGGAATTTGTGCAGCAAATGCAACTGCTAGGGAAGATGAGACATGTAAACCTTGTACAAATTCTCTCTTTCTACTATTCCAGGGAGGAGAAGCTCCTCATCTATAACTTTGTCCCTGGTGCCACTACTTTGTTTGAGCTTTTACATGGGAATAGAGGAGTTGGAAGAGTACCTTTGGCTTGGGTTACAAGACTATCAATTATCAAAGGCATAGCACAGGGTCTGATATTCCTTCACCAATCCTTGCGTTCACATAAGGTTCCTCATGCCAACCTTAAATCGTCAAATGTACTAGTTATCCCAGACGAAAACTACAATGTGAAGCTTGCAGATTTCGGATTCTTGCCACTCCTGCCTGCTCGAGAATGCTCGCAAAGCATTCTAGCGATCAGTAAGTCACCGGAGTTGGCTCAAGCGAAGAGGTTGTCACAGAAAGCCGATGTCTATTGCTTTGGCATTGTCCTACTGGAGGTCATTACTGGCAAGGTCCCAGGCGAAGTTTCACCGGGAAGTGATGAAACAATAATAGATGATCTTTCTGATTGGGTTAGAATGGTGGTAAACAATGACTGGTCCACAGATATTTTGGATATAGAAATGCTGGCAGCAAAAGATGACCATGATGACATGTTGAAGCTGACCAACATTGCCCTTGAGTGTACAGACATCTCCCCAGAGAACCGGCCTAAAATGATCCAAGTATTGAAAAGCATTCAAGAAATAGGGAGCAGCTCAAGACGATTTGATTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 Pkinase Pkinase_Tyr

Protein Analysis

625

Amino Acids

69.69

Weight (kDa)

5.41

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 340 - 610 1.8e-27 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 340 - 611 5.9e-27 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016852)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g18620
malus_domestica MD14G1247600.v1.1
prunus_persica Prupe.5G246000_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0176881
rosa_laevigata RLG00000005576
rosa_multiflora Rmu_co8477461.1_g000001 Rmu_sc0005310.1_g000052
rosa_roxburghii Rroxscaffold_3G00276320
rosa_rugosa Rorug06G0401300
rosa_samantha Rh7AG000800 Rh7BG001400 Rh7CG000800 Rh7DG001100
rosa_wichuraiana Rw7G000070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 80, 842
AccB1I GGYRCC 1 cut(s) 1241
AccI GTMKAC 2 cut(s) 74, 80
AciI CCGC 2 cut(s) 439, 533
AclWI GGATC 3 cut(s) 920, 933, 1810
AcoI YGGCCR 1 cut(s) 972
AcsI RAATTY 5 cut(s) 101, 511, 676, 1127, 1182
AcuI CTGAAG 1 cut(s) 108
AcyI GRCGYC 2 cut(s) 77, 839
AfaI GTAC 5 cut(s) 5, 1179, 1293, 1413, 1783
AfiI CCNNNNNNNGG 5 cut(s) 445, 522, 605, 1081, 1591
AflIII ACRYGT 2 cut(s) 1165, 1749
AgsI TTSAA 5 cut(s) 240, 416, 1756, 1828, 1838
AhlI ACTAGT 1 cut(s) 1414
AjiI CACGTC 1 cut(s) 250
AjnI CCWGG 4 cut(s) 486, 1202, 1237, 1612
AleI CACNNNNGTG 2 cut(s) 608, 655
Alw21I GWGCWC 2 cut(s) 155, 411
Alw26I GTCTC 4 cut(s) 135, 407, 587, 1156
Alw44I GTGCAC 1 cut(s) 407
AlwI GGATC 3 cut(s) 920, 933, 1810
AlwNI CAGNNNCTG 2 cut(s) 767, 1345
Ama87I CYCGRG 2 cut(s) 584, 1482
AoxI GGCC 3 cut(s) 972, 1072, 1805
ApaI GGGCCC 1 cut(s) 1076
ApaLI GTGCAC 1 cut(s) 407
ApeKI GCWGC 8 cut(s) 213, 502, 758, 761, 764, 1135, 1727, 1851
ApoI RAATTY 5 cut(s) 101, 511, 676, 1127, 1182
ArsI GACNNNNNNTTYG 2 cut(s) 1532, 1564
AspS9I GGNCC 6 cut(s) 188, 590, 1072, 1073, 1609, 1695
AsuC2I CCSGG 1 cut(s) 1629
AsuHPI GGTGA 5 cut(s) 288, 1099, 1349, 1512, 1617
AsuII TTCGAA 1 cut(s) 722
AvaI CYCGRG 2 cut(s) 584, 1482
AvaII GGWCC 4 cut(s) 188, 590, 1609, 1695
BaeGI GKGCMC 2 cut(s) 411, 1076
BamHI GGATCC 1 cut(s) 925
BanI GGYRCC 1 cut(s) 1241
BanII GRGCYC 2 cut(s) 155, 1076
BauI CACGAG 1 cut(s) 404
Bbv12I GWGCWC 2 cut(s) 155, 411
BbvI GCAGC 8 cut(s) 225, 489, 745, 773, 776, 1147, 1739, 1863
BccI CCATC 3 cut(s) 213, 515, 861
BcgI CGANNNNNNTGC 1 cut(s) 1851
BciT130I CCWGG 4 cut(s) 488, 1204, 1239, 1614
BclI TGATCA 1 cut(s) 42
BcnI CCSGG 1 cut(s) 1629
BcoDI GTCTC 4 cut(s) 135, 407, 587, 1156
BcuI ACTAGT 1 cut(s) 1414
BfaI CTAG 7 cut(s) 266, 497, 1064, 1151, 1415, 1505, 1873
BfmI CTRYAG 1 cut(s) 759
BisI GCNGC 8 cut(s) 214, 503, 759, 762, 765, 1136, 1728, 1852
BlpI GCTNAGC 2 cut(s) 276, 855
BlsI GCNGC 8 cut(s) 215, 504, 760, 763, 766, 1137, 1729, 1853
Bme1390I CCNGG 5 cut(s) 488, 1204, 1239, 1614, 1629
Bme18I GGWCC 4 cut(s) 188, 590, 1609, 1695
BmeT110I CYCGRG 2 cut(s) 584, 1482
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 6 cut(s) 188, 590, 1072, 1073, 1609, 1695
BmiI GGNNCC 7 cut(s) 591, 774, 927, 1074, 1243, 1383, 1611
BmrFI CCNGG 5 cut(s) 488, 1204, 1239, 1614, 1629
BmrI ACTGGG 1 cut(s) 1049
BmsI GCATC 1 cut(s) 879
BmuI ACTGGG 1 cut(s) 1049
BpmI CTGGAG 1 cut(s) 1610
Bpu10I CCTNAGC 1 cut(s) 344
Bpu1102I GCTNAGC 2 cut(s) 276, 855
Bpu14I TTCGAA 1 cut(s) 722
BpuEI CTTGAG 3 cut(s) 1518, 1796, 1840
BpuMI CCSGG 1 cut(s) 1629
BsaBI GATNNNNATC 1 cut(s) 900
BsaHI GRCGYC 2 cut(s) 77, 839
BsaJI CCNNGG 4 cut(s) 975, 1203, 1237, 1612
BsaWI WCCGGW 1 cut(s) 1522
BsaXI ACNNNNNCTCC 6 cut(s) 175, 205, 359, 389, 676, 706
Bsc4I CCNNNNNNNGG 5 cut(s) 445, 522, 605, 1081, 1591
Bse118I RCCGGY 2 cut(s) 970, 1803
Bse1I ACTGG 7 cut(s) 190, 248, 610, 1044, 1593, 1606, 1697
Bse3DI GCAATG 1 cut(s) 1767
Bse8I GATNNNNATC 1 cut(s) 900
BseBI CCWGG 4 cut(s) 488, 1204, 1239, 1614
BseDI CCNNGG 4 cut(s) 975, 1203, 1237, 1612
BseGI GGATG 4 cut(s) 161, 872, 916, 1000
BseJI GATNNNNATC 1 cut(s) 900
BseLI CCNNNNNNNGG 5 cut(s) 445, 522, 605, 1081, 1591
BseMI GCAATG 1 cut(s) 1767
BseMII CTCAG 7 cut(s) 128, 159, 290, 297, 335, 545, 869
BseNI ACTGG 7 cut(s) 190, 248, 610, 1044, 1593, 1606, 1697
BseRI GAGGAG 6 cut(s) 678, 681, 690, 1208, 1223, 1293
BseSI GKGCMC 2 cut(s) 411, 1076
BseX3I CGGCCG 1 cut(s) 972
BseXI GCAGC 8 cut(s) 225, 489, 745, 773, 776, 1147, 1739, 1863
BsgI GTGCAG 1 cut(s) 1154
Bsh1285I CGRYCG 1 cut(s) 975
BshFI GGCC 3 cut(s) 974, 1074, 1807
BshNI GGYRCC 1 cut(s) 1241
BsiEI CGRYCG 1 cut(s) 975
BsiHKAI GWGCWC 2 cut(s) 155, 411
BsiHKCI CYCGRG 2 cut(s) 584, 1482
BsiSI CCGG 5 cut(s) 378, 971, 1523, 1628, 1804
BslFI GGGAC 2 cut(s) 1220, 1595
BslI CCNNNNNNNGG 5 cut(s) 445, 522, 605, 1081, 1591
BsmAI GTCTC 4 cut(s) 135, 407, 587, 1156
BsmBI CGTCTC 1 cut(s) 587
BsmFI GGGAC 2 cut(s) 1220, 1595
BsmI GAATGC 3 cut(s) 1493, 1500, 1833
BsnI GGCC 3 cut(s) 974, 1074, 1807
BsoBI CYCGRG 2 cut(s) 584, 1482
Bsp119I TTCGAA 1 cut(s) 722
Bsp120I GGGCCC 1 cut(s) 1072
Bsp1286I GDGCHC 3 cut(s) 155, 411, 1076
Bsp1407I TGTACA 3 cut(s) 3, 1177, 1781
Bsp143I GATC 8 cut(s) 42, 475, 730, 847, 925, 1509, 1654, 1815
Bsp1720I GCTNAGC 2 cut(s) 276, 855
BspACI CCGC 2 cut(s) 439, 533
BspANI GGCC 3 cut(s) 974, 1074, 1807
BspCNI CTCAG 7 cut(s) 129, 158, 289, 296, 336, 546, 868
BspLI GGNNCC 7 cut(s) 591, 774, 927, 1074, 1243, 1383, 1611
BspMAI CTGCAG 1 cut(s) 763
BspPI GGATC 3 cut(s) 920, 933, 1810
BspT104I TTCGAA 1 cut(s) 722
BspT107I GGYRCC 1 cut(s) 1241
BsrDI GCAATG 1 cut(s) 1767
BsrFI RCCGGY 2 cut(s) 970, 1803
BsrGI TGTACA 3 cut(s) 3, 1177, 1781
BsrI ACTGG 7 cut(s) 190, 248, 610, 1044, 1593, 1606, 1697
BssAI RCCGGY 2 cut(s) 970, 1803
BssECI CCNNGG 4 cut(s) 975, 1203, 1237, 1612
BssMI GATC 8 cut(s) 42, 475, 730, 847, 925, 1509, 1654, 1815
BssNI GRCGYC 2 cut(s) 77, 839
BssSI CACGAG 1 cut(s) 404
Bst2BI CACGAG 1 cut(s) 404
Bst2UI CCWGG 4 cut(s) 488, 1204, 1239, 1614
Bst6I CTCTTC 5 cut(s) 315, 390, 1085, 1282, 1535
BstACI GRCGYC 2 cut(s) 77, 839
BstAPI GCANNNNNTGC 1 cut(s) 1141
BstAUI TGTACA 3 cut(s) 3, 1177, 1781
BstBI TTCGAA 1 cut(s) 722
BstC8I GCNNGC 6 cut(s) 304, 972, 1449, 1479, 1493, 1725
BstENI CCTNNNNNAGG 1 cut(s) 1589
BstF5I GGATG 4 cut(s) 161, 872, 916, 1000
BstKTI GATC 8 cut(s) 45, 478, 733, 850, 928, 1512, 1657, 1818
BstMAI GTCTC 4 cut(s) 135, 407, 587, 1156
BstMBI GATC 8 cut(s) 42, 475, 730, 847, 925, 1509, 1654, 1815
BstMCI CGRYCG 1 cut(s) 975
BstMWI GCNNNNNNNGC 6 cut(s) 354, 502, 764, 1141, 1474, 1497
BstNI CCWGG 4 cut(s) 488, 1204, 1239, 1614
BstNSI RCATGY 2 cut(s) 1169, 1753
BstSCI CCNGG 5 cut(s) 486, 1202, 1237, 1612, 1627
BstSFI CTRYAG 1 cut(s) 759
BstSLI GKGCMC 2 cut(s) 411, 1076
BstV1I GCAGC 8 cut(s) 225, 489, 745, 773, 776, 1147, 1739, 1863
BstX2I RGATCY 1 cut(s) 925
BstYI RGATCY 1 cut(s) 925
BstZI CGGCCG 1 cut(s) 972
BsuRI GGCC 3 cut(s) 974, 1074, 1807
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 4 cut(s) 161, 872, 916, 1000
BtsI GCAGTG 1 cut(s) 879
BtsIMutI CAGTG 4 cut(s) 153, 551, 603, 879
Cac8I GCNNGC 6 cut(s) 304, 972, 1449, 1479, 1493, 1725
CaiI CAGNNNCTG 2 cut(s) 767, 1345
Cfr10I RCCGGY 2 cut(s) 970, 1803
Cfr13I GGNCC 6 cut(s) 188, 590, 1072, 1073, 1609, 1695
Csp6I GTAC 5 cut(s) 4, 1178, 1292, 1412, 1782
CspCI CAANNNNNGTGG 2 cut(s) 729, 764
CviAII CATG 8 cut(s) 19, 821, 1105, 1166, 1268, 1388, 1742, 1750
CviQI GTAC 5 cut(s) 4, 1178, 1292, 1412, 1782
DpnI GATC 8 cut(s) 44, 477, 732, 849, 927, 1511, 1656, 1817
DpnII GATC 8 cut(s) 42, 475, 730, 847, 925, 1509, 1654, 1815
EaeI YGGCCR 1 cut(s) 972
EagI CGGCCG 1 cut(s) 972
Eam1104I CTCTTC 5 cut(s) 315, 390, 1085, 1282, 1535
EarI CTCTTC 5 cut(s) 315, 390, 1085, 1282, 1535
EciI GGCGGA 1 cut(s) 522
Ecl136II GAGCTC 1 cut(s) 153
EclXI CGGCCG 1 cut(s) 972
Eco24I GRGCYC 2 cut(s) 155, 1076
Eco47I GGWCC 4 cut(s) 188, 590, 1609, 1695
Eco52I CGGCCG 1 cut(s) 972
Eco53kI GAGCTC 1 cut(s) 153
Eco57I CTGAAG 1 cut(s) 108
Eco88I CYCGRG 2 cut(s) 584, 1482
EcoICRI GAGCTC 1 cut(s) 153
EcoNI CCTNNNNNAGG 1 cut(s) 1589
EcoO109I RGGNCCY 2 cut(s) 1073, 1609
EcoRI GAATTC 3 cut(s) 101, 511, 676
EcoRII CCWGG 4 cut(s) 486, 1202, 1237, 1612
EcoT38I GRGCYC 2 cut(s) 155, 1076
Esp3I CGTCTC 1 cut(s) 587
FaeI CATG 8 cut(s) 22, 824, 1108, 1169, 1271, 1391, 1745, 1753
FalI AAGNNNNNCTT 2 cut(s) 1279, 1311
FaqI GGGAC 2 cut(s) 1220, 1595
FatI CATG 8 cut(s) 18, 820, 1104, 1165, 1267, 1387, 1741, 1749
FbaI TGATCA 1 cut(s) 42
FblI GTMKAC 2 cut(s) 74, 80
Fnu4HI GCNGC 8 cut(s) 214, 503, 759, 762, 765, 1136, 1728, 1852
FokI GGATG 4 cut(s) 148, 879, 923, 1007
FriOI GRGCYC 2 cut(s) 155, 1076
Fsp4HI GCNGC 8 cut(s) 214, 503, 759, 762, 765, 1136, 1728, 1852
FspBI CTAG 7 cut(s) 266, 497, 1064, 1151, 1415, 1505, 1873
GluI GCNGC 8 cut(s) 214, 503, 759, 762, 765, 1136, 1728, 1852
GsuI CTGGAG 1 cut(s) 1610
HaeIII GGCC 3 cut(s) 974, 1074, 1807
HapII CCGG 5 cut(s) 378, 971, 1523, 1628, 1804
Hin1I GRCGYC 2 cut(s) 77, 839
Hin1II CATG 8 cut(s) 22, 824, 1108, 1169, 1271, 1391, 1745, 1753
HincII GTYRAC 3 cut(s) 75, 81, 843
HindII GTYRAC 3 cut(s) 75, 81, 843
HindIII AAGCTT 1 cut(s) 1445
HinfI GANTC 7 cut(s) 143, 529, 595, 664, 719, 1067, 1461
HpaII CCGG 5 cut(s) 378, 971, 1523, 1628, 1804
HphI GGTGA 5 cut(s) 288, 1099, 1349, 1512, 1617
Hpy166II GTNNAC 9 cut(s) 75, 81, 409, 843, 1171, 1373, 1684, 1698, 1782
Hpy188I TCNGA 8 cut(s) 42, 51, 88, 112, 594, 1347, 1460, 1663
Hpy188III TCNNGA 8 cut(s) 266, 343, 584, 625, 929, 1484, 1838, 1857
Hpy8I GTNNAC 9 cut(s) 75, 81, 409, 843, 1171, 1373, 1684, 1698, 1782
Hpy99I CGWCG 6 cut(s) 17, 70, 79, 82, 254, 841
HpyAV CCTTC 4 cut(s) 382, 705, 1117, 1364
HpyCH4IV ACGT 3 cut(s) 77, 249, 839
HpyF10VI GCNNNNNNNGC 6 cut(s) 354, 502, 764, 1141, 1474, 1497
HpySE526I ACGT 3 cut(s) 77, 249, 839
Hsp92I GRCGYC 2 cut(s) 77, 839
Hsp92II CATG 8 cut(s) 22, 824, 1108, 1169, 1271, 1391, 1745, 1753
KroI GCCGGC 1 cut(s) 970
KroNI GCCGGC 1 cut(s) 972
Ksp22I TGATCA 1 cut(s) 42
Kzo9I GATC 8 cut(s) 42, 475, 730, 847, 925, 1509, 1654, 1815
LmnI GCTCC 2 cut(s) 1221, 1848
Lsp1109I GCAGC 8 cut(s) 225, 489, 745, 773, 776, 1147, 1739, 1863
LweI GCATC 1 cut(s) 879
MaeI CTAG 7 cut(s) 266, 497, 1064, 1151, 1415, 1505, 1873
MaeII ACGT 3 cut(s) 77, 249, 839
MaeIII GTNAC 6 cut(s) 612, 803, 1057, 1306, 1518, 1548
MalI GATC 8 cut(s) 44, 477, 732, 849, 927, 1511, 1656, 1817
MboI GATC 8 cut(s) 42, 475, 730, 847, 925, 1509, 1654, 1815
MboII GAAGA 9 cut(s) 125, 281, 302, 377, 898, 1102, 1168, 1299, 1552
MflI RGATCY 1 cut(s) 925
MhlI GDGCHC 3 cut(s) 155, 411, 1076
MluCI AATT 9 cut(s) 33, 101, 178, 511, 676, 1095, 1127, 1182, 1320
MlyI GAGTC 2 cut(s) 137, 673
MmeI TCCRAC 2 cut(s) 927, 1264
MroNI GCCGGC 1 cut(s) 970
MseI TTAA 3 cut(s) 207, 564, 1398
MslI CAYNNNNRTG 4 cut(s) 23, 608, 655, 657
MspA1I CMGCKG 2 cut(s) 302, 767
MspI CCGG 5 cut(s) 378, 971, 1523, 1628, 1804
MspR9I CCNGG 5 cut(s) 488, 1204, 1239, 1614, 1629
Mva1269I GAATGC 3 cut(s) 1493, 1500, 1833
MvaI CCWGG 4 cut(s) 488, 1204, 1239, 1614
MwoI GCNNNNNNNGC 6 cut(s) 354, 502, 764, 1141, 1474, 1497
NaeI GCCGGC 1 cut(s) 972
NciI CCSGG 1 cut(s) 1629
NdeII GATC 8 cut(s) 42, 475, 730, 847, 925, 1509, 1654, 1815
NgoMIV GCCGGC 1 cut(s) 970
NlaIII CATG 8 cut(s) 22, 824, 1108, 1169, 1271, 1391, 1745, 1753
NlaIV GGNNCC 7 cut(s) 591, 774, 927, 1074, 1243, 1383, 1611
NmeAIII GCCGAG 1 cut(s) 1000
NmuCI GTSAC 3 cut(s) 1057, 1518, 1548
NspI RCATGY 2 cut(s) 1169, 1753
NspV TTCGAA 1 cut(s) 722
OliI CACNNNNGTG 2 cut(s) 608, 655
PaeR7I CTCGAG 2 cut(s) 584, 1482
PciI ACATGT 2 cut(s) 1165, 1749
PcsI WCGNNNNNNNCGW 2 cut(s) 71, 74
PctI GAATGC 3 cut(s) 1493, 1500, 1833
PdiI GCCGGC 1 cut(s) 972
PfeI GAWTC 5 cut(s) 529, 595, 719, 1067, 1461
PkrI GCNGC 8 cut(s) 215, 504, 760, 763, 766, 1137, 1729, 1853
PleI GAGTC 2 cut(s) 137, 672
PpsI GAGTC 2 cut(s) 137, 672
PpuMI RGGWCCY 1 cut(s) 1609
PscI ACATGT 2 cut(s) 1165, 1749
Psp124BI GAGCTC 1 cut(s) 155
Psp5II RGGWCCY 1 cut(s) 1609
Psp6I CCWGG 4 cut(s) 486, 1202, 1237, 1612
PspGI CCWGG 4 cut(s) 486, 1202, 1237, 1612
PspN4I GGNNCC 7 cut(s) 591, 774, 927, 1074, 1243, 1383, 1611
PspOMI GGGCCC 1 cut(s) 1072
PspPI GGNCC 6 cut(s) 188, 590, 1072, 1073, 1609, 1695
PspPPI RGGWCCY 1 cut(s) 1609
PsrI GAACNNNNNNTAC 2 cut(s) 220, 252
PstI CTGCAG 1 cut(s) 763
PstNI CAGNNNCTG 2 cut(s) 767, 1345
PsuI RGATCY 1 cut(s) 925
PvuII CAGCTG 2 cut(s) 302, 767
RsaI GTAC 5 cut(s) 5, 1179, 1293, 1413, 1783
RsaNI GTAC 5 cut(s) 4, 1178, 1292, 1412, 1782
RseI CAYNNNNRTG 4 cut(s) 23, 608, 655, 657
SacI GAGCTC 1 cut(s) 155
SalI GTCGAC 2 cut(s) 73, 79
SaqAI TTAA 3 cut(s) 207, 564, 1398
SatI GCNGC 8 cut(s) 214, 503, 759, 762, 765, 1136, 1728, 1852
Sau3AI GATC 8 cut(s) 42, 475, 730, 847, 925, 1509, 1654, 1815
Sau96I GGNCC 6 cut(s) 188, 590, 1072, 1073, 1609, 1695
SchI GAGTC 2 cut(s) 137, 673
ScrFI CCNGG 5 cut(s) 488, 1204, 1239, 1614, 1629
SduI GDGCHC 3 cut(s) 155, 411, 1076
SfaNI GCATC 1 cut(s) 879
SfcI CTRYAG 1 cut(s) 759
Sfr274I CTCGAG 2 cut(s) 584, 1482
SfuI TTCGAA 1 cut(s) 722
SinI GGWCC 4 cut(s) 188, 590, 1609, 1695
SlaI CTCGAG 2 cut(s) 584, 1482
SmiMI CAYNNNNRTG 4 cut(s) 23, 608, 655, 657
SmlI CTYRAG 5 cut(s) 584, 1482, 1533, 1775, 1855
SmoI CTYRAG 5 cut(s) 584, 1482, 1533, 1775, 1855
SpeI ACTAGT 1 cut(s) 1414
Sse9I AATT 9 cut(s) 33, 101, 178, 511, 676, 1095, 1127, 1182, 1320
SsiI CCGC 2 cut(s) 439, 533
SspMI CTAG 7 cut(s) 266, 497, 1064, 1151, 1415, 1505, 1873
SstI GAGCTC 1 cut(s) 155
StyD4I CCNGG 5 cut(s) 486, 1202, 1237, 1612, 1627
TaiI ACGT 3 cut(s) 80, 252, 842
TasI AATT 9 cut(s) 33, 101, 178, 511, 676, 1095, 1127, 1182, 1320
TatI WGTACW 4 cut(s) 3, 1177, 1411, 1781
TfiI GAWTC 5 cut(s) 529, 595, 719, 1067, 1461
Tru1I TTAA 3 cut(s) 207, 564, 1398
Tru9I TTAA 3 cut(s) 207, 564, 1398
TscAI CASTG 4 cut(s) 153, 558, 610, 879
TseFI GTSAC 3 cut(s) 1057, 1518, 1548
TseI GCWGC 8 cut(s) 213, 502, 758, 761, 764, 1135, 1727, 1851
Tsp45I GTSAC 3 cut(s) 1057, 1518, 1548
TspDTI ATGAA 6 cut(s) 114, 245, 846, 1038, 1121, 1653
TspRI CASTG 4 cut(s) 153, 558, 610, 879
VneI GTGCAC 1 cut(s) 407
VpaK11BI GGWCC 4 cut(s) 188, 590, 1609, 1695
XagI CCTNNNNNAGG 1 cut(s) 1589
XapI RAATTY 5 cut(s) 101, 511, 676, 1127, 1182
XbaI TCTAGA 1 cut(s) 265
XceI RCATGY 2 cut(s) 1169, 1753
XcmI CCANNNNNNNNNTGG 1 cut(s) 1705
XhoI CTCGAG 2 cut(s) 584, 1482
XmiI GTMKAC 2 cut(s) 74, 80
XspI CTAG 7 cut(s) 266, 497, 1064, 1151, 1415, 1505, 1873
ZraI GACGTC 2 cut(s) 78, 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.