Prupe.5G246000_v2.0.a1

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0016852
Physical Location & Seq
Forward (+)
18405127 .. 18407251
2125 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G246000.1

Sequence Viewer

Length: 1782 bp
ATGAATTTCGTTGCATCGGTATCTTCCGAAATTGATGAATACTACCCAGAAGAAAGAGAAGCACTATTGAAGCTGAGAGATTCTCTAAGCTCAAACTCAGATTTGCACGGGAATTGGACTGGTCCTCCATGTATCAATAACAGCAGCAGATGGGCTGGTATTGCTTGTTCAAATTGGCATGTTGTTCACATTGTCCTGCAAGGAATTCAGCTAGCAGGTTCCCTTCCACCTTGTGCATTTCTACATAACATAACTTTCTTGAGCAAACTTAGCCTTAGAAACAACACACTCTCCGGCCCTGTTCCAAACCTCACAAACCTTGTCTACTTGGAATATGTGGTTTTGTCCTACAACCGCTTCTGGGGTTCAATTCCTTTGGAGTATATTGAATTGCCTAACCTCAGAGTGCTAGAGTTGCAGAGCAATTATTTAGATGGACACATTCCACCTTTTGACCAACCCACCTTGAGAGCATTTAATGTTTCTTATAATCATCTTGAGGGTCCGATTCCTCAAACTGCTGTGTTACAGAAGTTCCCCAAAAGCTCCTACGATCATAACTCAAATCTTTGTGGGAGTCCTTTGGAAACATCATGTCCACCTCCGCCTCCAGTAGTCATTGCGCCATCTCCTTCACCCCTTTCCCCAAATATTATTCCACCCAAGAAGAAGAACAATCTCCAAGAATGGAGTTTTGCTTTGATCGTGGCTGCTGCAGCGCTGGTTCTGTTTCTCGTCATATTTGTTTCCTTATGTTATTCCAGGAAGGTGCATAGAAAACAAGCAGCAAAGGAGGAACAACAGACAGGAGATGGTACTTCAGGGTGGGCACAAACGAAAATGCCACATTTGGAGAGCTTGGGGGATCCTGAAAAGAAGGTTGAGTTGGATTTTTTTGACAAGGAAATGCCAGCAGCTTTTGACCTGGATGATTTACTAAGGGCATCTGCACAAGTGTTGGGCAAGGGGAAACTAGGCACTACATACAAAGTAACACTGGAATCGGGTCCTGTTGTGGTGGTGAAGAGACTTAAAAACATGAATGAGTTGAGCAAAAAGGAATTTACCCAACAGATGCAATTGCTAGGGAATATGAGGCATGAAAACCTTGTACAGATTCTCTCTTTCTACTATTCCAAGGAGGAGAAGCTGGTCATCTCTAAGTTTGAGTCTAGTGGCACTTTGTTTGAACTTTTACATGAGAACAGAGGAGTTGGAAGAGTACCTTTGGATTGGGTAACAAGACTATCAATAATCAAAGACATAGCAAAGGGTCTCACATTCCTTCACCAATCCTTGCCTTCACATAAGGTCCCTCATGCCAACCTGAAATCGTCTAATGTACTAGTCATCCAGCGAAACAGCCAAACCTACCATTTGAAGCTTACAGATTTCGGATTCTTACCACTTGTGCCCTCTCGAAAGTACAATGAAGATCTAGCCATCGGCAAGTCACCCGAGTTTGCTCAAGGGAAGAAGTTGTCACAGAAATCCGATGTCTATTGCTTTGGTATAATCCTATTGGAGGTCATTACTGGCAGGATTCCAGGTGAAATCTCACCGGGAAATGATGAAACGATGGATGATCTTTCTGATTGGGTTAGAATGGTGGTGAACAATGACTGGTCCACAGATATTCTGGATGTAGAGATACTGGCAGCAAAGGAAGACCATGATGAGATGCTGAAGCTCACCAAGATTGCACTAGACTGTACAGACTTGACACCGGAGAAGCGGCCAAAAATGATTCAAGTGTTGACAAGAATAGAAGAGATTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 Pkinase Pkinase_Tyr

Protein Analysis

594

Amino Acids

66.55

Weight (kDa)

6.09

Isoelectric Point (pI)

48.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016852)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g18620
malus_domestica MD14G1247600.v1.1
prunus_persica Prupe.5G246000_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0176881
rosa_laevigata RLG00000005576
rosa_multiflora Rmu_co8477461.1_g000001 Rmu_sc0005310.1_g000052
rosa_roxburghii Rroxscaffold_3G00276320
rosa_rugosa Rorug06G0401300
rosa_samantha Rh7AG000800 Rh7BG001400 Rh7CG000800 Rh7DG001100
rosa_wichuraiana Rw7G000070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 489
Acc36I ACCTGC 1 cut(s) 206
AccI GTMKAC 1 cut(s) 324
AciI CCGC 3 cut(s) 355, 605, 1735
AclWI GGATC 2 cut(s) 860, 873
AcoI YGGCCR 1 cut(s) 1736
AcsI RAATTY 3 cut(s) 4, 204, 1061
AcuI CTGAAG 2 cut(s) 804, 1706
AdeI CACNNNGTG 1 cut(s) 233
AfaI GTAC 6 cut(s) 817, 1113, 1224, 1344, 1427, 1714
AfeI AGCGCT 1 cut(s) 720
AfiI CCNNNNNNNGG 2 cut(s) 361, 1525
AgsI TTSAA 7 cut(s) 70, 171, 369, 389, 1190, 1381, 1751
AhlI ACTAGT 1 cut(s) 1345
AjnI CCWGG 3 cut(s) 761, 924, 1546
AjuI GAANNNNNNNTTGG 2 cut(s) 869, 901
AluBI AGCT 9 cut(s) 73, 90, 211, 546, 858, 917, 1150, 1384, 1690
AluI AGCT 9 cut(s) 73, 90, 211, 546, 858, 917, 1150, 1384, 1690
Alw26I GTCTC 2 cut(s) 1021, 1280
AlwI GGATC 2 cut(s) 860, 873
Ama87I CYCGRG 1 cut(s) 1457
Aor51HI AGCGCT 1 cut(s) 720
AoxI GGCC 2 cut(s) 295, 1736
ApeKI GCWGC 7 cut(s) 144, 710, 713, 716, 785, 914, 1658
ApoI RAATTY 3 cut(s) 4, 204, 1061
AspLEI GCGC 2 cut(s) 625, 721
AspS9I GGNCC 6 cut(s) 122, 296, 503, 1007, 1312, 1626
AsuC2I CCSGG 1 cut(s) 1563
AsuHPI GGTGA 8 cut(s) 627, 1033, 1280, 1446, 1551, 1562, 1624, 1684
AsuNHI GCTAGC 1 cut(s) 211
AvaI CYCGRG 1 cut(s) 1457
AvaII GGWCC 5 cut(s) 122, 503, 1007, 1312, 1626
BaeGI GKGCMC 2 cut(s) 832, 1416
BamHI GGATCC 1 cut(s) 865
BbsI GAAGAC 1 cut(s) 1674
BbvI GCAGC 7 cut(s) 156, 697, 700, 728, 797, 926, 1670
BccI CCATC 6 cut(s) 144, 428, 634, 806, 1451, 1573
BciT130I CCWGG 3 cut(s) 763, 926, 1548
BcnI CCSGG 1 cut(s) 1563
BcoDI GTCTC 2 cut(s) 1021, 1280
BcuI ACTAGT 1 cut(s) 1345
BfaI CTAG 8 cut(s) 212, 410, 974, 1085, 1173, 1346, 1439, 1706
BfmI CTRYAG 1 cut(s) 714
BfoI RGCGCY 1 cut(s) 722
BfuAI ACCTGC 1 cut(s) 206
BglII AGATCT 1 cut(s) 1435
BisI GCNGC 8 cut(s) 145, 711, 714, 717, 786, 915, 1659, 1736
BlsI GCNGC 8 cut(s) 146, 712, 715, 718, 787, 916, 1660, 1737
Bme1390I CCNGG 4 cut(s) 763, 926, 1548, 1563
Bme18I GGWCC 5 cut(s) 122, 503, 1007, 1312, 1626
BmeT110I CYCGRG 1 cut(s) 1457
BmgT120I GGNCC 6 cut(s) 122, 296, 503, 1007, 1312, 1626
BmiI GGNNCC 5 cut(s) 220, 504, 867, 1008, 1314
BmrFI CCNGG 4 cut(s) 763, 926, 1548, 1563
BmsI GCATC 4 cut(s) 23, 953, 1065, 1671
BmtI GCTAGC 1 cut(s) 215
BpiI GAAGAC 1 cut(s) 1674
BplI GAGNNNNNCTC 2 cut(s) 67, 99
BpmI CTGGAG 1 cut(s) 594
BpuEI CTTGAG 4 cut(s) 280, 487, 518, 1452
BpuMI CCSGG 1 cut(s) 1563
BsaI GGTCTC 1 cut(s) 1280
BsaJI CCNNGG 1 cut(s) 1137
BsaWI WCCGGW 1 cut(s) 1726
BsaXI ACNNNNNCTCC 4 cut(s) 109, 139, 275, 305
Bsc4I CCNNNNNNNGG 2 cut(s) 361, 1525
Bse1I ACTGG 6 cut(s) 124, 611, 1002, 1540, 1628, 1659
Bse3DI GCAATG 1 cut(s) 618
BseBI CCWGG 3 cut(s) 763, 926, 1548
BseDI CCNNGG 1 cut(s) 1137
BseGI GGATG 4 cut(s) 934, 1350, 1588, 1648
BseLI CCNNNNNNNGG 2 cut(s) 361, 1525
BseMI GCAATG 1 cut(s) 618
BseMII CTCAG 3 cut(s) 65, 111, 415
BseNI ACTGG 6 cut(s) 124, 611, 1002, 1540, 1628, 1659
BseRI GAGGAG 2 cut(s) 1157, 1224
BseSI GKGCMC 2 cut(s) 832, 1416
BseXI GCAGC 7 cut(s) 156, 697, 700, 728, 797, 926, 1670
BsgI GTGCAG 1 cut(s) 933
BshFI GGCC 2 cut(s) 297, 1738
BsiHKCI CYCGRG 1 cut(s) 1457
BsiSI CCGG 3 cut(s) 294, 1562, 1727
BslFI GGGAC 1 cut(s) 1298
BslI CCNNNNNNNGG 2 cut(s) 361, 1525
BsmAI GTCTC 2 cut(s) 1021, 1280
BsmFI GGGAC 1 cut(s) 1298
BsnI GGCC 2 cut(s) 297, 1738
Bso31I GGTCTC 1 cut(s) 1280
BsoBI CYCGRG 1 cut(s) 1457
Bsp1286I GDGCHC 2 cut(s) 832, 1416
Bsp1407I TGTACA 2 cut(s) 1111, 1712
Bsp143I GATC 5 cut(s) 553, 702, 865, 1435, 1585
BspACI CCGC 3 cut(s) 355, 605, 1735
BspANI GGCC 2 cut(s) 297, 1738
BspCNI CTCAG 3 cut(s) 66, 110, 414
BspLI GGNNCC 5 cut(s) 220, 504, 867, 1008, 1314
BspMAI CTGCAG 1 cut(s) 718
BspMI ACCTGC 1 cut(s) 206
BspOI GCTAGC 1 cut(s) 215
BspPI GGATC 2 cut(s) 860, 873
BspTNI GGTCTC 1 cut(s) 1280
BsrDI GCAATG 1 cut(s) 618
BsrGI TGTACA 2 cut(s) 1111, 1712
BsrI ACTGG 6 cut(s) 124, 611, 1002, 1540, 1628, 1659
BssECI CCNNGG 1 cut(s) 1137
BssMI GATC 5 cut(s) 553, 702, 865, 1435, 1585
BssT1I CCWWGG 1 cut(s) 1137
Bst2UI CCWGG 3 cut(s) 763, 926, 1548
Bst4CI ACNGT 1 cut(s) 1712
Bst6I CTCTTC 3 cut(s) 1019, 1213, 1764
BstAUI TGTACA 2 cut(s) 1111, 1712
BstC8I GCNNGC 2 cut(s) 213, 912
BstDEI CTNAG 8 cut(s) 74, 86, 97, 269, 275, 401, 938, 1161
BstENI CCTNNNNNAGG 1 cut(s) 1523
BstF5I GGATG 4 cut(s) 934, 1350, 1588, 1648
BstH2I RGCGCY 1 cut(s) 722
BstHHI GCGC 2 cut(s) 625, 721
BstKTI GATC 5 cut(s) 556, 705, 868, 1438, 1588
BstMAI GTCTC 2 cut(s) 1021, 1280
BstMBI GATC 5 cut(s) 553, 702, 865, 1435, 1585
BstMWI GCNNNNNNNGC 4 cut(s) 161, 270, 415, 716
BstNI CCWGG 3 cut(s) 763, 926, 1548
BstNSI RCATGY 1 cut(s) 182
BstSCI CCNGG 4 cut(s) 761, 924, 1546, 1561
BstSFI CTRYAG 1 cut(s) 714
BstSLI GKGCMC 2 cut(s) 832, 1416
BstV1I GCAGC 7 cut(s) 156, 697, 700, 728, 797, 926, 1670
BstV2I GAAGAC 1 cut(s) 1674
BstX2I RGATCY 2 cut(s) 865, 1435
BstYI RGATCY 2 cut(s) 865, 1435
BsuRI GGCC 2 cut(s) 297, 1738
BtsCI GGATG 4 cut(s) 934, 1350, 1588, 1648
BtsIMutI CAGTG 1 cut(s) 995
BveI ACCTGC 1 cut(s) 206
Cac8I GCNNGC 2 cut(s) 213, 912
CfoI GCGC 2 cut(s) 625, 721
Cfr13I GGNCC 6 cut(s) 122, 296, 503, 1007, 1312, 1626
Csp6I GTAC 6 cut(s) 816, 1112, 1223, 1343, 1426, 1713
CviAII CATG 8 cut(s) 129, 179, 594, 1039, 1100, 1199, 1319, 1673
CviQI GTAC 6 cut(s) 816, 1112, 1223, 1343, 1426, 1713
DdeI CTNAG 8 cut(s) 74, 86, 97, 269, 275, 401, 938, 1161
DpnI GATC 5 cut(s) 555, 704, 867, 1437, 1587
DpnII GATC 5 cut(s) 553, 702, 865, 1435, 1585
DraIII CACNNNGTG 1 cut(s) 233
EaeI YGGCCR 1 cut(s) 1736
Eam1104I CTCTTC 3 cut(s) 1019, 1213, 1764
EarI CTCTTC 3 cut(s) 1019, 1213, 1764
EciI GGCGGA 1 cut(s) 594
Eco130I CCWWGG 1 cut(s) 1137
Eco31I GGTCTC 1 cut(s) 1280
Eco47I GGWCC 5 cut(s) 122, 503, 1007, 1312, 1626
Eco47III AGCGCT 1 cut(s) 720
Eco57I CTGAAG 2 cut(s) 804, 1706
Eco88I CYCGRG 1 cut(s) 1457
EcoNI CCTNNNNNAGG 1 cut(s) 1523
EcoO109I RGGNCCY 2 cut(s) 1007, 1312
EcoRI GAATTC 1 cut(s) 204
EcoRII CCWGG 3 cut(s) 761, 924, 1546
EcoT14I CCWWGG 1 cut(s) 1137
ErhI CCWWGG 1 cut(s) 1137
FaeI CATG 8 cut(s) 132, 182, 597, 1042, 1103, 1202, 1322, 1676
FalI AAGNNNNNCTT 2 cut(s) 1210, 1242
FaqI GGGAC 1 cut(s) 1298
FatI CATG 8 cut(s) 128, 178, 593, 1038, 1099, 1198, 1318, 1672
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 8 cut(s) 145, 711, 714, 717, 786, 915, 1659, 1736
FokI GGATG 4 cut(s) 941, 1337, 1595, 1655
Fsp4HI GCNGC 8 cut(s) 145, 711, 714, 717, 786, 915, 1659, 1736
FspBI CTAG 8 cut(s) 212, 410, 974, 1085, 1173, 1346, 1439, 1706
GlaI GCGC 2 cut(s) 624, 720
GluI GCNGC 8 cut(s) 145, 711, 714, 717, 786, 915, 1659, 1736
GsuI CTGGAG 1 cut(s) 594
HaeII RGCGCY 1 cut(s) 722
HaeIII GGCC 2 cut(s) 297, 1738
HapII CCGG 3 cut(s) 294, 1562, 1727
HhaI GCGC 2 cut(s) 625, 721
Hin1II CATG 8 cut(s) 132, 182, 597, 1042, 1103, 1202, 1322, 1676
Hin6I GCGC 2 cut(s) 623, 719
HinP1I GCGC 2 cut(s) 623, 719
HincII GTYRAC 1 cut(s) 1758
HindII GTYRAC 1 cut(s) 1758
HindIII AAGCTT 1 cut(s) 1382
HinfI GANTC 9 cut(s) 80, 508, 577, 1001, 1117, 1169, 1398, 1543, 1747
HpaII CCGG 3 cut(s) 294, 1562, 1727
HphI GGTGA 8 cut(s) 627, 1033, 1280, 1446, 1551, 1562, 1624, 1684
Hpy166II GTNNAC 6 cut(s) 187, 325, 599, 1615, 1629, 1758
Hpy188I TCNGA 7 cut(s) 28, 100, 404, 507, 1397, 1495, 1594
Hpy188III TCNNGA 5 cut(s) 259, 497, 869, 1419, 1640
Hpy8I GTNNAC 6 cut(s) 187, 325, 599, 1615, 1629, 1758
HpyAV CCTTC 6 cut(s) 233, 642, 760, 871, 1295, 1311
HpyCH4III ACNGT 1 cut(s) 1712
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 270, 415, 716
HpyF3I CTNAG 8 cut(s) 74, 86, 97, 269, 275, 401, 938, 1161
Hsp92II CATG 8 cut(s) 132, 182, 597, 1042, 1103, 1202, 1322, 1676
HspAI GCGC 2 cut(s) 623, 719
Kzo9I GATC 5 cut(s) 553, 702, 865, 1435, 1585
LmnI GCTCC 1 cut(s) 551
Lsp1109I GCAGC 7 cut(s) 156, 697, 700, 728, 797, 926, 1670
LweI GCATC 4 cut(s) 23, 953, 1065, 1671
MaeI CTAG 8 cut(s) 212, 410, 974, 1085, 1173, 1346, 1439, 1706
MaeIII GTNAC 5 cut(s) 525, 991, 1237, 1452, 1482
MalI GATC 5 cut(s) 555, 704, 867, 1437, 1587
MboI GATC 5 cut(s) 553, 702, 865, 1435, 1585
MfeI CAATTG 1 cut(s) 1079
MflI RGATCY 2 cut(s) 865, 1435
MhlI GDGCHC 2 cut(s) 832, 1416
MlyI GAGTC 2 cut(s) 586, 1178
MmeI TCCRAC 2 cut(s) 867, 1195
MseI TTAA 2 cut(s) 477, 1032
MspI CCGG 3 cut(s) 294, 1562, 1727
MspR9I CCNGG 4 cut(s) 763, 926, 1548, 1563
MunI CAATTG 1 cut(s) 1079
MvaI CCWGG 3 cut(s) 763, 926, 1548
MwoI GCNNNNNNNGC 4 cut(s) 161, 270, 415, 716
NciI CCSGG 1 cut(s) 1563
NdeII GATC 5 cut(s) 553, 702, 865, 1435, 1585
NheI GCTAGC 1 cut(s) 211
NlaIII CATG 8 cut(s) 132, 182, 597, 1042, 1103, 1202, 1322, 1676
NlaIV GGNNCC 5 cut(s) 220, 504, 867, 1008, 1314
NmuCI GTSAC 2 cut(s) 1452, 1482
NspI RCATGY 1 cut(s) 182
PfeI GAWTC 7 cut(s) 80, 508, 1001, 1117, 1398, 1543, 1747
PfoI TCCNGGA 1 cut(s) 761
PkrI GCNGC 8 cut(s) 146, 712, 715, 718, 787, 916, 1660, 1737
PleI GAGTC 2 cut(s) 585, 1177
PpsI GAGTC 2 cut(s) 585, 1177
PpuMI RGGWCCY 2 cut(s) 1007, 1312
PsiI TTATAA 1 cut(s) 489
Psp5II RGGWCCY 2 cut(s) 1007, 1312
Psp6I CCWGG 3 cut(s) 761, 924, 1546
PspGI CCWGG 3 cut(s) 761, 924, 1546
PspN4I GGNNCC 5 cut(s) 220, 504, 867, 1008, 1314
PspPI GGNCC 6 cut(s) 122, 296, 503, 1007, 1312, 1626
PspPPI RGGWCCY 2 cut(s) 1007, 1312
PsrI GAACNNNNNNTAC 2 cut(s) 151, 183
PstI CTGCAG 1 cut(s) 718
PsuI RGATCY 2 cut(s) 865, 1435
RsaI GTAC 6 cut(s) 817, 1113, 1224, 1344, 1427, 1714
RsaNI GTAC 6 cut(s) 816, 1112, 1223, 1343, 1426, 1713
SaqAI TTAA 2 cut(s) 477, 1032
SatI GCNGC 8 cut(s) 145, 711, 714, 717, 786, 915, 1659, 1736
Sau3AI GATC 5 cut(s) 553, 702, 865, 1435, 1585
Sau96I GGNCC 6 cut(s) 122, 296, 503, 1007, 1312, 1626
SchI GAGTC 2 cut(s) 586, 1178
ScrFI CCNGG 4 cut(s) 763, 926, 1548, 1563
SduI GDGCHC 2 cut(s) 832, 1416
SfaNI GCATC 4 cut(s) 23, 953, 1065, 1671
SfcI CTRYAG 1 cut(s) 714
SinI GGWCC 5 cut(s) 122, 503, 1007, 1312, 1626
SmlI CTYRAG 4 cut(s) 259, 466, 497, 1467
SmoI CTYRAG 4 cut(s) 259, 466, 497, 1467
SpeI ACTAGT 1 cut(s) 1345
SsiI CCGC 3 cut(s) 355, 605, 1735
SspI AATATT 1 cut(s) 652
SspMI CTAG 8 cut(s) 212, 410, 974, 1085, 1173, 1346, 1439, 1706
StyD4I CCNGG 4 cut(s) 761, 924, 1546, 1561
StyI CCWWGG 1 cut(s) 1137
TaaI ACNGT 1 cut(s) 1712
TaqI TCGA 1 cut(s) 1420
TatI WGTACW 4 cut(s) 1111, 1342, 1425, 1712
TauI GCSGC 1 cut(s) 1738
TfiI GAWTC 7 cut(s) 80, 508, 1001, 1117, 1398, 1543, 1747
Tru1I TTAA 2 cut(s) 477, 1032
Tru9I TTAA 2 cut(s) 477, 1032
TscAI CASTG 1 cut(s) 1002
TseFI GTSAC 2 cut(s) 1452, 1482
TseI GCWGC 7 cut(s) 144, 710, 713, 716, 785, 914, 1658
Tsp45I GTSAC 2 cut(s) 1452, 1482
TspDTI ATGAA 6 cut(s) 17, 51, 1055, 1116, 1446, 1587
TspRI CASTG 1 cut(s) 1002
VpaK11BI GGWCC 5 cut(s) 122, 503, 1007, 1312, 1626
XagI CCTNNNNNAGG 1 cut(s) 1523
XapI RAATTY 3 cut(s) 4, 204, 1061
XceI RCATGY 1 cut(s) 182
XcmI CCANNNNNNNNNTGG 1 cut(s) 1636
XmiI GTMKAC 1 cut(s) 324
XspI CTAG 8 cut(s) 212, 410, 974, 1085, 1173, 1346, 1439, 1706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.