Rh7CG000800

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0016852
Physical Location & Seq
Reverse (-)
121711 .. 123806
2096 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG000800.1

Sequence Viewer

Length: 1875 bp
ATGATGACCTCTTCTAAAAACATGTACATACTCTTTGCGATGATGAGGATGAGTTTTCTGATCAATCCGATATTAACATCATCGTCGTCGTGGACCTCGGAAGGATTTGATGAATACTACTCAGAAGAAAGAGAGGCTTTACTAAGGTTAAGAGACTCTGTGAGCTCAAACTCCTCCAACTTGCACTCAAATTGGACCGGCCCTCCCTGCAATAACATTAACAGCAGCAAATGGTCTGGTATTGCTTGTTCAAATTGGCACGTTGTTCACCTTGTCCTAGAAGGAACTCGGCTAGCAGCTACTATAGCACCAGGTGGGTCTCTTCCACCCACATTTCTCCAAAACATAACTTTCCTGAGCAAACTTAGCTTCAGAAACAACACCTTCTCTGGCTCTCTTCCAAACCTATCAAATCTTGTTCACCTGGAGTATGTGGATTTCTCATACAACCGTTTCTGGGGTTCCATACCTTTCGAGTACACTACTGATTTGCCAAAGCTGGAACTGCTAGAGTTGCAAGAGAATTCTTTAGATGGGTCAATTCCGCCTTTCGACCAACCAACACTCAGAGCTTTTAATGTTTCTCATAATCATCTCGACGGTCCAATTCCTAACACTGGTGTGTTACAGAAGTTCCCCAAAAGCTCGTACGATCATAATGCAAATCTTTGTGGGAGTCCTCTGGGGATATCTTGTACTCCTCCTCCATCTCCTAATTCCCCGCCAAACGATTCAAAAGATGACCTCCCACTCGCTTTGATTGTGGCTGCAGCAGCGGCGGTTCCTTTTTTTGTTTTATTTGTATTCTTATGTTATTATAAGAAAGTGCATGGAAAAGATGAAGCGACGTCAACAGATCAGGCCGAGCAGACGGAGTGGGCAGTGGCACAAAAGAAAATGCCTAATGATGATCATTTGGATGAGAGGTTGGGGGATCCTGAAAAGAAGGTCGAGTTGGAATTTTTCGACAAGGACTTGCCCGCCGAGGCATTTTTCGATTTGGATGATTTACTTCGGGCTTCAGCCGAAGTGTTAGGCAGGGGAAAACTGGGAACTTCATACAAAGTAACACTAGAATCGGGCCCTGTCGTGGTGGTGAAGAGACTAAGAAGCATGAACGAGTTGAGCAAAAAGGAATTTGTCCAGCAAATGCAATTGCTAGGGAAGATGAGACACGAAAACCTTGTACAGATTCTCTCCTTCTACTATTCCAAGGAGGAGAAGCTCGTCATCTATAAGTTTGTCTCTGGTGCCACTACTTTGTTTGAGCTTTTACATGGTAATAGAGAAGTTGGAAGAGTAGCTTTGGCTTGGGCAACAAGACTATCAATTATCAAAAGCATAGCAAAGGGTCTGATATTCCTTCACCAATCCTTGCGTTCACATAAGGTTCCTCATGCCAACCTTAAATCGTCAAATGTACTAGTTGTTATCCGAGACGAAAACTACCATGTGAAGCTCACAGATTTCGGATTCTTGCCACTCTTGCCTGCTCGAGAATGCTCACAAAATAGCATTCTAGCAATTGGTAAGTCACCGGAGTTGGCTCAAGCGAAGAAGTTGTCACAGAAAGCCGATGTCTATTGCTTTGGCATTGTCCTACTGGAGGTCATTACTGGCAGGATCCCAGGCAAAATCTCACCGGGAAGTGATGAAACAATTGATGATCTTTCCGATTGGGTTAGAATGGTGGTAAACAATGATTGGTCCACAGATATCTTGGATGTAGAAATGCTGGCAGCAAAAGATGACCACGATGACATGTTGAAGCTGACCAACATTGCCCTAGAGTGTACAGACATCGCCCCAGAGAACCGGCCTAAAATGATTCAAGTATTGAAAAGCATTCAAGAAATAGAGAGTAGTTCAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 Pkinase Pkinase_Tyr

Protein Analysis

624

Amino Acids

69.76

Weight (kDa)

5.72

Isoelectric Point (pI)

44.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 343 - 615 1.2e-28 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 343 - 614 2e-28 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016852)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g18620
malus_domestica MD14G1247600.v1.1
prunus_persica Prupe.5G246000_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0176881
rosa_laevigata RLG00000005576
rosa_multiflora Rmu_co8477461.1_g000001 Rmu_sc0005310.1_g000052
rosa_roxburghii Rroxscaffold_3G00276320
rosa_rugosa Rorug06G0401300
rosa_samantha Rh7AG000800 Rh7BG001400 Rh7CG000800 Rh7DG001100
rosa_wichuraiana Rw7G000070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 819
AatII GACGTC 1 cut(s) 851
AccB1I GGYRCC 1 cut(s) 1250
AciI CCGC 5 cut(s) 545, 722, 776, 779, 981
AclWI GGATC 4 cut(s) 929, 942, 1618, 1631
AcsI RAATTY 3 cut(s) 523, 959, 1136
AcuI CTGAAG 2 cut(s) 355, 1005
AcyI GRCGYC 1 cut(s) 848
AdeI CACNNNGTG 1 cut(s) 314
AfaI GTAC 7 cut(s) 26, 479, 650, 697, 1188, 1422, 1795
AfiI CCNNNNNNNGG 4 cut(s) 457, 617, 1090, 1606
AflIII ACRYGT 2 cut(s) 21, 1761
AgsI TTSAA 7 cut(s) 252, 735, 1768, 1832, 1840, 1850, 1869
AhlI ACTAGT 1 cut(s) 1423
AjnI CCWGG 3 cut(s) 310, 423, 1627
AjuI GAANNNNNNNTTGG 2 cut(s) 938, 970
AleI CACNNNNGTG 1 cut(s) 620
Alw21I GWGCWC 1 cut(s) 167
Alw26I GTCTC 6 cut(s) 147, 324, 1096, 1165, 1249, 1431
AlwI GGATC 4 cut(s) 929, 942, 1618, 1631
Ama87I CYCGRG 1 cut(s) 1494
AoxI GGCC 4 cut(s) 199, 861, 1081, 1817
ApaI GGGCCC 1 cut(s) 1085
ApeKI GCWGC 6 cut(s) 225, 296, 767, 770, 773, 1739
ApoI RAATTY 3 cut(s) 523, 959, 1136
ArsI GACNNNNNNTTYG 2 cut(s) 1547, 1579
AspS9I GGNCC 7 cut(s) 93, 195, 200, 602, 1081, 1082, 1707
AsuC2I CCSGG 1 cut(s) 1644
AsuHPI GGTGA 6 cut(s) 260, 413, 1108, 1358, 1527, 1632
AsuNHI GCTAGC 1 cut(s) 292
AvaI CYCGRG 1 cut(s) 1494
AvaII GGWCC 4 cut(s) 93, 195, 602, 1707
BaeGI GKGCMC 1 cut(s) 1085
BamHI GGATCC 2 cut(s) 934, 1623
BanI GGYRCC 1 cut(s) 1250
BanII GRGCYC 2 cut(s) 167, 1085
Bbv12I GWGCWC 1 cut(s) 167
BbvI GCAGC 6 cut(s) 237, 308, 754, 782, 785, 1751
BccI CCATC 2 cut(s) 527, 715
BcgI CGANNNNNNTGC 2 cut(s) 641, 675
BciT130I CCWGG 3 cut(s) 312, 425, 1629
BclI TGATCA 2 cut(s) 60, 910
BcnI CCSGG 1 cut(s) 1644
BcoDI GTCTC 6 cut(s) 147, 324, 1096, 1165, 1249, 1431
BcuI ACTAGT 1 cut(s) 1423
BfaI CTAG 8 cut(s) 278, 293, 509, 1073, 1160, 1424, 1520, 1787
BfmI CTRYAG 2 cut(s) 303, 768
BisI GCNGC 7 cut(s) 226, 297, 768, 771, 774, 777, 1740
BlsI GCNGC 7 cut(s) 227, 298, 769, 772, 775, 778, 1741
Bme1390I CCNGG 4 cut(s) 312, 425, 1629, 1644
Bme18I GGWCC 4 cut(s) 93, 195, 602, 1707
BmeT110I CYCGRG 1 cut(s) 1494
BmgT120I GGNCC 7 cut(s) 93, 195, 200, 602, 1081, 1082, 1707
BmiI GGNNCC 7 cut(s) 463, 783, 936, 1083, 1252, 1392, 1625
BmrFI CCNGG 4 cut(s) 312, 425, 1629, 1644
BmrI ACTGGG 1 cut(s) 1058
BmtI GCTAGC 1 cut(s) 296
BmuI ACTGGG 1 cut(s) 1058
BpmI CTGGAG 2 cut(s) 446, 1625
Bpu10I CCTNAGC 1 cut(s) 356
BpuEI CTTGAG 1 cut(s) 1533
BpuMI CCSGG 1 cut(s) 1644
BsaHI GRCGYC 1 cut(s) 848
BsaI GGTCTC 1 cut(s) 324
BsaJI CCNNGG 4 cut(s) 96, 984, 1212, 1627
BsaWI WCCGGW 1 cut(s) 1537
BsaXI ACNNNNNCTCC 4 cut(s) 187, 217, 688, 718
Bsc4I CCNNNNNNNGG 4 cut(s) 457, 617, 1090, 1606
Bse118I RCCGGY 2 cut(s) 197, 1815
Bse1I ACTGG 4 cut(s) 622, 1053, 1608, 1621
Bse3DI GCAATG 1 cut(s) 1779
BseBI CCWGG 3 cut(s) 312, 425, 1629
BseDI CCNNGG 4 cut(s) 96, 984, 1212, 1627
BseGI GGATG 4 cut(s) 54, 925, 1009, 1729
BseLI CCNNNNNNNGG 4 cut(s) 457, 617, 1090, 1606
BseMI GCAATG 1 cut(s) 1779
BseMII CTCAG 3 cut(s) 135, 347, 580
BseNI ACTGG 4 cut(s) 622, 1053, 1608, 1621
BseRI GAGGAG 4 cut(s) 163, 690, 693, 1232
BseSI GKGCMC 1 cut(s) 1085
BseXI GCAGC 6 cut(s) 237, 308, 754, 782, 785, 1751
BshFI GGCC 4 cut(s) 201, 863, 1083, 1819
BshNI GGYRCC 1 cut(s) 1250
BsiHKAI GWGCWC 1 cut(s) 167
BsiHKCI CYCGRG 1 cut(s) 1494
BsiSI CCGG 4 cut(s) 198, 1538, 1643, 1816
BsiWI CGTACG 1 cut(s) 648
BslI CCNNNNNNNGG 4 cut(s) 457, 617, 1090, 1606
BsmAI GTCTC 6 cut(s) 147, 324, 1096, 1165, 1249, 1431
BsmBI CGTCTC 1 cut(s) 1431
BsmI GAATGC 3 cut(s) 1505, 1515, 1845
BsnI GGCC 4 cut(s) 201, 863, 1083, 1819
Bso31I GGTCTC 1 cut(s) 324
BsoBI CYCGRG 1 cut(s) 1494
Bsp120I GGGCCC 1 cut(s) 1081
Bsp1286I GDGCHC 2 cut(s) 167, 1085
Bsp1407I TGTACA 3 cut(s) 24, 1186, 1793
Bsp143I GATC 7 cut(s) 60, 652, 856, 910, 934, 1623, 1666
BspACI CCGC 5 cut(s) 545, 722, 776, 779, 981
BspANI GGCC 4 cut(s) 201, 863, 1083, 1819
BspCNI CTCAG 3 cut(s) 134, 348, 579
BspLI GGNNCC 7 cut(s) 463, 783, 936, 1083, 1252, 1392, 1625
BspMAI CTGCAG 1 cut(s) 772
BspOI GCTAGC 1 cut(s) 296
BspPI GGATC 4 cut(s) 929, 942, 1618, 1631
BspT107I GGYRCC 1 cut(s) 1250
BspTNI GGTCTC 1 cut(s) 324
BsrDI GCAATG 1 cut(s) 1779
BsrFI RCCGGY 2 cut(s) 197, 1815
BsrGI TGTACA 3 cut(s) 24, 1186, 1793
BsrI ACTGG 4 cut(s) 622, 1053, 1608, 1621
BssAI RCCGGY 2 cut(s) 197, 1815
BssECI CCNNGG 4 cut(s) 96, 984, 1212, 1627
BssMI GATC 7 cut(s) 60, 652, 856, 910, 934, 1623, 1666
BssNI GRCGYC 1 cut(s) 848
BssT1I CCWWGG 1 cut(s) 1212
Bst2UI CCWGG 3 cut(s) 312, 425, 1629
Bst4CI ACNGT 2 cut(s) 452, 602
Bst6I CTCTTC 5 cut(s) 16, 327, 402, 1094, 1291
BstACI GRCGYC 1 cut(s) 848
BstAUI TGTACA 3 cut(s) 24, 1186, 1793
BstC8I GCNNGC 4 cut(s) 294, 981, 1491, 1737
BstDEI CTNAG 6 cut(s) 121, 143, 356, 365, 566, 1106
BstENI CCTNNNNNAGG 1 cut(s) 1604
BstF5I GGATG 4 cut(s) 54, 925, 1009, 1729
BstKTI GATC 7 cut(s) 63, 655, 859, 913, 937, 1626, 1669
BstMAI GTCTC 6 cut(s) 147, 324, 1096, 1165, 1249, 1431
BstMBI GATC 7 cut(s) 60, 652, 856, 910, 934, 1623, 1666
BstMWI GCNNNNNNNGC 8 cut(s) 207, 305, 366, 505, 514, 773, 776, 1486
BstNI CCWGG 3 cut(s) 312, 425, 1629
BstNSI RCATGY 2 cut(s) 25, 1765
BstSCI CCNGG 4 cut(s) 310, 423, 1627, 1642
BstSFI CTRYAG 2 cut(s) 303, 768
BstSLI GKGCMC 1 cut(s) 1085
BstV1I GCAGC 6 cut(s) 237, 308, 754, 782, 785, 1751
BstX2I RGATCY 2 cut(s) 934, 1623
BstYI RGATCY 2 cut(s) 934, 1623
BsuRI GGCC 4 cut(s) 201, 863, 1083, 1819
BtgZI GCGATG 2 cut(s) 53, 1786
BtsCI GGATG 4 cut(s) 54, 925, 1009, 1729
BtsI GCAGTG 1 cut(s) 888
BtsIMutI CAGTG 2 cut(s) 615, 888
Cac8I GCNNGC 4 cut(s) 294, 981, 1491, 1737
Cfr10I RCCGGY 2 cut(s) 197, 1815
Cfr13I GGNCC 7 cut(s) 93, 195, 200, 602, 1081, 1082, 1707
CsiI ACCWGGT 1 cut(s) 310
Csp6I GTAC 7 cut(s) 25, 478, 649, 696, 1187, 1421, 1794
CspCI CAANNNNNGTGG 2 cut(s) 738, 773
CviAII CATG 7 cut(s) 22, 830, 1114, 1277, 1397, 1451, 1762
CviQI GTAC 7 cut(s) 25, 478, 649, 696, 1187, 1421, 1794
DdeI CTNAG 6 cut(s) 121, 143, 356, 365, 566, 1106
DpnI GATC 7 cut(s) 62, 654, 858, 912, 936, 1625, 1668
DpnII GATC 7 cut(s) 60, 652, 856, 910, 934, 1623, 1666
DraIII CACNNNGTG 1 cut(s) 314
Eam1104I CTCTTC 5 cut(s) 16, 327, 402, 1094, 1291
EarI CTCTTC 5 cut(s) 16, 327, 402, 1094, 1291
EciI GGCGGA 1 cut(s) 534
Ecl136II GAGCTC 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 1212
Eco24I GRGCYC 2 cut(s) 167, 1085
Eco31I GGTCTC 1 cut(s) 324
Eco32I GATATC 2 cut(s) 690, 1717
Eco47I GGWCC 4 cut(s) 93, 195, 602, 1707
Eco53kI GAGCTC 1 cut(s) 165
Eco57I CTGAAG 2 cut(s) 355, 1005
Eco88I CYCGRG 1 cut(s) 1494
EcoICRI GAGCTC 1 cut(s) 165
EcoNI CCTNNNNNAGG 1 cut(s) 1604
EcoO109I RGGNCCY 1 cut(s) 1082
EcoRI GAATTC 1 cut(s) 523
EcoRII CCWGG 3 cut(s) 310, 423, 1627
EcoRV GATATC 2 cut(s) 690, 1717
EcoT14I CCWWGG 1 cut(s) 1212
EcoT38I GRGCYC 2 cut(s) 167, 1085
ErhI CCWWGG 1 cut(s) 1212
Esp3I CGTCTC 1 cut(s) 1431
FaeI CATG 7 cut(s) 25, 833, 1117, 1280, 1400, 1454, 1765
FalI AAGNNNNNCTT 4 cut(s) 121, 153, 1288, 1320
FatI CATG 7 cut(s) 21, 829, 1113, 1276, 1396, 1450, 1761
FauI CCCGC 2 cut(s) 729, 988
FbaI TGATCA 2 cut(s) 60, 910
Fnu4HI GCNGC 7 cut(s) 226, 297, 768, 771, 774, 777, 1740
FokI GGATG 4 cut(s) 61, 932, 1016, 1736
FriOI GRGCYC 2 cut(s) 167, 1085
Fsp4HI GCNGC 7 cut(s) 226, 297, 768, 771, 774, 777, 1740
FspBI CTAG 8 cut(s) 278, 293, 509, 1073, 1160, 1424, 1520, 1787
GluI GCNGC 7 cut(s) 226, 297, 768, 771, 774, 777, 1740
GsuI CTGGAG 2 cut(s) 446, 1625
HaeIII GGCC 4 cut(s) 201, 863, 1083, 1819
HapII CCGG 4 cut(s) 198, 1538, 1643, 1816
Hin1I GRCGYC 1 cut(s) 848
Hin1II CATG 7 cut(s) 25, 833, 1117, 1280, 1400, 1454, 1765
HincII GTYRAC 1 cut(s) 852
HindII GTYRAC 1 cut(s) 852
HinfI GANTC 7 cut(s) 155, 676, 731, 1076, 1192, 1473, 1828
HpaII CCGG 4 cut(s) 198, 1538, 1643, 1816
HphI GGTGA 6 cut(s) 260, 413, 1108, 1358, 1527, 1632
Hpy166II GTNNAC 9 cut(s) 93, 268, 421, 480, 852, 1382, 1696, 1710, 1794
Hpy188III TCNNGA 6 cut(s) 355, 596, 938, 1496, 1850, 1869
Hpy8I GTNNAC 9 cut(s) 93, 268, 421, 480, 852, 1382, 1696, 1710, 1794
Hpy99I CGWCG 4 cut(s) 88, 91, 602, 850
HpyAV CCTTC 6 cut(s) 95, 275, 394, 940, 1210, 1373
HpyCH4III ACNGT 2 cut(s) 452, 602
HpyCH4IV ACGT 2 cut(s) 261, 848
HpyCH4V TGCA 7 cut(s) 184, 210, 517, 662, 770, 829, 1153
HpyF10VI GCNNNNNNNGC 8 cut(s) 207, 305, 366, 505, 514, 773, 776, 1486
HpyF3I CTNAG 6 cut(s) 121, 143, 356, 365, 566, 1106
HpySE526I ACGT 2 cut(s) 261, 848
Hsp92I GRCGYC 1 cut(s) 848
Hsp92II CATG 7 cut(s) 25, 833, 1117, 1280, 1400, 1454, 1765
Ksp22I TGATCA 2 cut(s) 60, 910
Kzo9I GATC 7 cut(s) 60, 652, 856, 910, 934, 1623, 1666
Lsp1109I GCAGC 6 cut(s) 237, 308, 754, 782, 785, 1751
MabI ACCWGGT 1 cut(s) 310
MaeI CTAG 8 cut(s) 278, 293, 509, 1073, 1160, 1424, 1520, 1787
MaeII ACGT 2 cut(s) 261, 848
MaeIII GTNAC 4 cut(s) 624, 1066, 1533, 1563
MalI GATC 7 cut(s) 62, 654, 858, 912, 936, 1625, 1668
MboI GATC 7 cut(s) 60, 652, 856, 910, 934, 1623, 1666
MboII GAAGA 8 cut(s) 3, 137, 314, 389, 1111, 1177, 1308, 1567
MfeI CAATTG 3 cut(s) 1154, 1524, 1659
MflI RGATCY 2 cut(s) 934, 1623
MhlI GDGCHC 2 cut(s) 167, 1085
MlyI GAGTC 2 cut(s) 149, 685
MmeI TCCRAC 3 cut(s) 201, 936, 1273
MseI TTAA 5 cut(s) 74, 149, 219, 576, 1407
MslI CAYNNNNRTG 2 cut(s) 620, 918
MspA1I CMGCKG 1 cut(s) 776
MspI CCGG 4 cut(s) 198, 1538, 1643, 1816
MspR9I CCNGG 4 cut(s) 312, 425, 1629, 1644
MunI CAATTG 3 cut(s) 1154, 1524, 1659
Mva1269I GAATGC 3 cut(s) 1505, 1515, 1845
MvaI CCWGG 3 cut(s) 312, 425, 1629
MwoI GCNNNNNNNGC 8 cut(s) 207, 305, 366, 505, 514, 773, 776, 1486
NciI CCSGG 1 cut(s) 1644
NdeII GATC 7 cut(s) 60, 652, 856, 910, 934, 1623, 1666
NheI GCTAGC 1 cut(s) 292
NlaIII CATG 7 cut(s) 25, 833, 1117, 1280, 1400, 1454, 1765
NlaIV GGNNCC 7 cut(s) 463, 783, 936, 1083, 1252, 1392, 1625
NmeAIII GCCGAG 3 cut(s) 268, 889, 1009
NmuCI GTSAC 2 cut(s) 1533, 1563
NspI RCATGY 2 cut(s) 25, 1765
OliI CACNNNNGTG 1 cut(s) 620
PaeR7I CTCGAG 1 cut(s) 1494
PciI ACATGT 2 cut(s) 21, 1761
PctI GAATGC 3 cut(s) 1505, 1515, 1845
PfeI GAWTC 5 cut(s) 731, 1076, 1192, 1473, 1828
Pfl23II CGTACG 1 cut(s) 648
PkrI GCNGC 7 cut(s) 227, 298, 769, 772, 775, 778, 1741
PleI GAGTC 2 cut(s) 149, 684
PpsI GAGTC 2 cut(s) 149, 684
PscI ACATGT 2 cut(s) 21, 1761
PsiI TTATAA 1 cut(s) 819
Psp124BI GAGCTC 1 cut(s) 167
Psp6I CCWGG 3 cut(s) 310, 423, 1627
PspGI CCWGG 3 cut(s) 310, 423, 1627
PspLI CGTACG 1 cut(s) 648
PspN4I GGNNCC 7 cut(s) 463, 783, 936, 1083, 1252, 1392, 1625
PspOMI GGGCCC 1 cut(s) 1081
PspPI GGNCC 7 cut(s) 93, 195, 200, 602, 1081, 1082, 1707
PsrI GAACNNNNNNTAC 2 cut(s) 232, 264
PstI CTGCAG 1 cut(s) 772
PsuI RGATCY 2 cut(s) 934, 1623
RsaI GTAC 7 cut(s) 26, 479, 650, 697, 1188, 1422, 1795
RsaNI GTAC 7 cut(s) 25, 478, 649, 696, 1187, 1421, 1794
RseI CAYNNNNRTG 2 cut(s) 620, 918
SacI GAGCTC 1 cut(s) 167
SaqAI TTAA 5 cut(s) 74, 149, 219, 576, 1407
SatI GCNGC 7 cut(s) 226, 297, 768, 771, 774, 777, 1740
Sau3AI GATC 7 cut(s) 60, 652, 856, 910, 934, 1623, 1666
Sau96I GGNCC 7 cut(s) 93, 195, 200, 602, 1081, 1082, 1707
SchI GAGTC 2 cut(s) 149, 685
ScrFI CCNGG 4 cut(s) 312, 425, 1629, 1644
SduI GDGCHC 2 cut(s) 167, 1085
SexAI ACCWGGT 1 cut(s) 310
SfcI CTRYAG 2 cut(s) 303, 768
Sfr274I CTCGAG 1 cut(s) 1494
SinI GGWCC 4 cut(s) 93, 195, 602, 1707
SlaI CTCGAG 1 cut(s) 1494
SmiMI CAYNNNNRTG 2 cut(s) 620, 918
SmlI CTYRAG 2 cut(s) 1494, 1548
SmoI CTYRAG 2 cut(s) 1494, 1548
SpeI ACTAGT 1 cut(s) 1423
SsiI CCGC 5 cut(s) 545, 722, 776, 779, 981
SspMI CTAG 8 cut(s) 278, 293, 509, 1073, 1160, 1424, 1520, 1787
SstI GAGCTC 1 cut(s) 167
StyD4I CCNGG 4 cut(s) 310, 423, 1627, 1642
StyI CCWWGG 1 cut(s) 1212
TaaI ACNGT 2 cut(s) 452, 602
TaiI ACGT 2 cut(s) 264, 851
TaqI TCGA 7 cut(s) 474, 552, 597, 951, 966, 996, 1495
TatI WGTACW 6 cut(s) 24, 477, 695, 1186, 1420, 1793
TauI GCSGC 1 cut(s) 779
TfiI GAWTC 5 cut(s) 731, 1076, 1192, 1473, 1828
Tru1I TTAA 5 cut(s) 74, 149, 219, 576, 1407
Tru9I TTAA 5 cut(s) 74, 149, 219, 576, 1407
TscAI CASTG 2 cut(s) 622, 888
TseFI GTSAC 2 cut(s) 1533, 1563
TseI GCWGC 6 cut(s) 225, 296, 767, 770, 773, 1739
Tsp45I GTSAC 2 cut(s) 1533, 1563
TspDTI ATGAA 5 cut(s) 126, 855, 1047, 1130, 1668
TspGWI ACGGA 1 cut(s) 887
TspRI CASTG 2 cut(s) 622, 888
VpaK11BI GGWCC 4 cut(s) 93, 195, 602, 1707
XagI CCTNNNNNAGG 1 cut(s) 1604
XapI RAATTY 3 cut(s) 523, 959, 1136
XceI RCATGY 2 cut(s) 25, 1765
XcmI CCANNNNNNNNNTGG 1 cut(s) 1717
XhoI CTCGAG 1 cut(s) 1494
XspI CTAG 8 cut(s) 278, 293, 509, 1073, 1160, 1424, 1520, 1787
ZraI GACGTC 1 cut(s) 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.