MD14G1247600.v1.1

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016852
Physical Location & Seq
Forward (+)
32503438 .. 32505971
2534 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1247600.v1.1.491

Sequence Viewer

Length: 1905 bp
ATGAATCTGCGTAAAACTAAAAGCGCACTAATTGTTATTGTGATGCTGATTTTATACAGAGAGAATCCATTAACATCAGCTGTGAATTTTGTTACATCTTCTTCAGAAGTTGATGAATACTACCCAGAAGAAAGAGACGCGCTGTTGAAGCTGAGAGATTCCGTAAGCTCGAACTCCAATTTGCATGGGAACTGGAGCGGTCCGCCGTGCATCAACAACAGCAGCAGATGGGCTGGTATCGCTTGTTCAAATGGGCACGTTGTTCACATTGCCCTCCAAGGAATCCAGCTGGCAGGTTCCCTTCCACCCGCATTTCTACATAACATAACTTTGTTGAGCAAACTTACCCTTTCAAACAATACTCTATCCGGCCCTCTTCCAAATCTCTCAAATCTGGTTTACTTGGAAGATGTGCTTCTGTCTTACAATTACTTCTGGGGTTCAATTCCTTTGGAGTATACTGAATTGCCTAACCTCAGAGTGCTTGAGTTGCAAGACAACTTTTTGGATGGCAAAATTCCACCTTTTGGCCAGCCCACGCTGAGAGGATTCAATGTTTCTTATAATCATCTCGTGGGTCCAATTCCTCAAACGGGTGTGCTGCAAAAATTCCCAATAAGCTCCTACTCTCATAACTCAAATCTTTGTGGAATTCCATTGGAGACACCATGTCCACTAGTTCCACCTCCATCTCCATCTCCGGCCATCATTGCGCCATCTCCTTCCTCAAATATTATTCCACCCAAGAACAAGAACAATCTCCAAGTGTGGAGTGTTGCTTTGATTGCCACTGCAGCTGCGCTCGTTCCATTTATCGTCATATTTGTTTTCTTATGTTATTACGGGAAGATGCAAACAAAAGAAGCGGCAAAGGAGGAACAGGCAGCAATAGGAGATGGTTCGTCAGGGTGGGTAGAAAAGAAAATGCCACATTTGGAGAGGTTGGGGGATCCTGAAAAGAGGGTTGAGTTAGATTTTTTTGACAAGGAGATGCCAGCAGCTTTTGACCTGGATGATTTACTAAGGGCATCTGCACAAGTGTTAGGCAAGGGGAAACTTGGCTCTACATACAAGGTAGCACTGGAATCGGGTCTTGTTGTCATCGTGAAGAGACTTAAAAACATGAATGAGTTGAGCAAAAAGGAATTTACCCAGCAGATGCAATTGCTAGGAAATATGAGGCATGAAAACCTTGTACGGATTCTCTCTTTTTACTTTTCCAAGGAGGAGAAGCTGGTCATCTCTAAGTTCGTCTCTGGCGGCCGCACTTTGTTTCAACTCTTACATGAGGACAGAGGAGTAGGAAGAGTAGCTTTGGATTGGGCAACAAGACTGTCGATAATCAAAGACATAGCACAGGGTCTCACATTCCTTCACCAATCCTTGCCTTCCCAAAAGGTCCCTCATGCCAACCTCAATTCGTCTAATGTACTCGTCATCCAACGAAGAAACAGCCAAATCTATGATTTGAAGCTTACAGATTTTGGATTCTTACCACTCCTGCCCTCGCGAAAGTCCTCCGAGGATTTAGCCATCAGCAAGTCACCAGAGTTTGCTCAAGGGAAGAAGTTGTCTCAGAAAGCCGATGTCTATTGCTTTGGTATAATCCTACTGGAGGTTGTTACTGGAAGGATCCCGGGTGAAGTTTCTCCGGGAAATGATGAAATCTTTGATGATCTTTCCGATTGGGTTAGAATGGTGGTGAACAATGATTGGTCTACTGATATTCTGGATGTAGAGATACTGGCGGCAAAGGATGACCACGATGACATGTTGAAGCTGACACAGATTGCGCTAGACTGTACAGACATGACACCAGAGAAGAGGCCTAAGATGGTCCAAGTATTGAAAAGAATACAAGAGATTAATTCACTCAAGATCCAAGAAATGACATACAGAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 Pkinase Pkinase_Tyr

Protein Analysis

635

Amino Acids

70.65

Weight (kDa)

6.35

Isoelectric Point (pI)

43.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 346 - 618 1.2e-28 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 346 - 617 2.1e-28 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016852)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g18620
malus_domestica MD14G1247600.v1.1
prunus_persica Prupe.5G246000_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0176881
rosa_laevigata RLG00000005576
rosa_multiflora Rmu_co8477461.1_g000001 Rmu_sc0005310.1_g000052
rosa_roxburghii Rroxscaffold_3G00276320
rosa_rugosa Rorug06G0401300
rosa_samantha Rh7AG000800 Rh7BG001400 Rh7CG000800 Rh7DG001100
rosa_wichuraiana Rw7G000070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 564
Acc36I ACCTGC 1 cut(s) 284
AccB7I CCANNNNNTGG 1 cut(s) 527
AccBSI CCGCTC 1 cut(s) 198
AccI GTMKAC 2 cut(s) 458, 1718
AccII CGCG 2 cut(s) 140, 1510
AciI CCGC 7 cut(s) 198, 203, 309, 866, 1260, 1264, 1748
AclWI GGATC 5 cut(s) 944, 957, 1627, 1640, 1873
AcoI YGGCCR 3 cut(s) 529, 702, 1261
AcsI RAATTY 5 cut(s) 85, 516, 608, 651, 1145
AcuI CTGAAG 1 cut(s) 87
AfaI GTAC 3 cut(s) 1197, 1431, 1804
AfiI CCNNNNNNNGG 3 cut(s) 527, 593, 1615
AflIII ACRYGT 1 cut(s) 1770
AgsI TTSAA 9 cut(s) 148, 249, 354, 444, 553, 1277, 1471, 1777, 1849
AhdI GACNNNNNGTC 1 cut(s) 669
AhlI ACTAGT 1 cut(s) 676
AjnI CCWGG 1 cut(s) 1008
Alw26I GTCTC 6 cut(s) 129, 656, 1105, 1258, 1367, 1578
AlwI GGATC 5 cut(s) 944, 957, 1627, 1640, 1873
Ama87I CYCGRG 1 cut(s) 1636
AoxI GGCC 5 cut(s) 370, 529, 702, 1261, 1826
ApeKI GCWGC 6 cut(s) 222, 601, 794, 797, 884, 998
ApoI RAATTY 5 cut(s) 85, 516, 608, 651, 1145
AseI ATTAAT 1 cut(s) 1866
AspLEI GCGC 5 cut(s) 26, 142, 715, 802, 1795
AspS9I GGNCC 5 cut(s) 200, 371, 578, 1399, 1837
AsuC2I CCSGG 3 cut(s) 1637, 1638, 1653
AsuHPI GGTGA 4 cut(s) 1367, 1536, 1652, 1714
AvaI CYCGRG 1 cut(s) 1636
AvaII GGWCC 4 cut(s) 200, 578, 1399, 1837
BaeGI GKGCMC 1 cut(s) 258
BalI TGGCCA 1 cut(s) 531
BamHI GGATCC 2 cut(s) 949, 1632
BauI CACGAG 1 cut(s) 572
BbvI GCAGC 6 cut(s) 234, 588, 784, 806, 896, 1010
BccI CCATC 9 cut(s) 222, 503, 697, 703, 713, 724, 890, 1541, 1828
BceAI ACGGC 1 cut(s) 190
BcgI CGANNNNNNTGC 2 cut(s) 1068, 1102
BciT130I CCWGG 1 cut(s) 1010
BcnI CCSGG 3 cut(s) 1637, 1638, 1653
BcoDI GTCTC 6 cut(s) 129, 656, 1105, 1258, 1367, 1578
BcuI ACTAGT 1 cut(s) 676
BfaI CTAG 3 cut(s) 677, 1169, 1796
BfmI CTRYAG 1 cut(s) 792
BfuAI ACCTGC 1 cut(s) 284
Bme1390I CCNGG 4 cut(s) 1010, 1637, 1638, 1653
Bme18I GGWCC 4 cut(s) 200, 578, 1399, 1837
BmeRI GACNNNNNGTC 1 cut(s) 669
BmeT110I CYCGRG 1 cut(s) 1636
BmgT120I GGNCC 5 cut(s) 200, 371, 578, 1399, 1837
BmiI GGNNCC 5 cut(s) 298, 579, 951, 1401, 1634
BmrFI CCNGG 4 cut(s) 1010, 1637, 1638, 1653
BmsI GCATC 6 cut(s) 33, 219, 840, 981, 1037, 1149
BpmI CTGGAG 2 cut(s) 214, 1634
BpuEI CTTGAG 3 cut(s) 506, 1542, 1859
BpuMI CCSGG 3 cut(s) 1637, 1638, 1653
BsaI GGTCTC 1 cut(s) 1367
BsaJI CCNNGG 4 cut(s) 277, 1221, 1521, 1636
Bsc4I CCNNNNNNNGG 3 cut(s) 527, 593, 1615
Bse1I ACTGG 5 cut(s) 197, 1086, 1617, 1630, 1749
Bse3DI GCAATG 2 cut(s) 267, 708
BseBI CCWGG 1 cut(s) 1010
BseDI CCNNGG 4 cut(s) 277, 1221, 1521, 1636
BseGI GGATG 5 cut(s) 514, 1018, 1437, 1738, 1762
BseLI CCNNNNNNNGG 3 cut(s) 527, 593, 1615
BseMI GCAATG 2 cut(s) 267, 708
BseMII CTCAG 4 cut(s) 143, 490, 533, 1589
BseNI ACTGG 5 cut(s) 197, 1086, 1617, 1630, 1749
BseRI GAGGAG 2 cut(s) 1241, 1311
BseSI GKGCMC 1 cut(s) 258
BseX3I CGGCCG 1 cut(s) 1261
BseXI GCAGC 6 cut(s) 234, 588, 784, 806, 896, 1010
BseYI CCCAGC 1 cut(s) 1152
BsgI GTGCAG 1 cut(s) 1017
Bsh1236I CGCG 2 cut(s) 140, 1510
Bsh1285I CGRYCG 1 cut(s) 1264
BshFI GGCC 5 cut(s) 372, 531, 704, 1263, 1828
BsiEI CGRYCG 1 cut(s) 1264
BsiHKCI CYCGRG 1 cut(s) 1636
BsiSI CCGG 4 cut(s) 369, 701, 1637, 1652
BslFI GGGAC 1 cut(s) 1385
BslI CCNNNNNNNGG 3 cut(s) 527, 593, 1615
BsmAI GTCTC 6 cut(s) 129, 656, 1105, 1258, 1367, 1578
BsmBI CGTCTC 2 cut(s) 129, 1258
BsmFI GGGAC 1 cut(s) 1385
BsnI GGCC 5 cut(s) 372, 531, 704, 1263, 1828
Bso31I GGTCTC 1 cut(s) 1367
BsoBI CYCGRG 1 cut(s) 1636
Bsp1286I GDGCHC 1 cut(s) 258
Bsp1407I TGTACA 1 cut(s) 1802
Bsp143I GATC 4 cut(s) 949, 1632, 1675, 1878
Bsp68I TCGCGA 1 cut(s) 1510
BspACI CCGC 7 cut(s) 198, 203, 309, 866, 1260, 1264, 1748
BspANI GGCC 5 cut(s) 372, 531, 704, 1263, 1828
BspCNI CTCAG 4 cut(s) 144, 489, 534, 1588
BspFNI CGCG 2 cut(s) 140, 1510
BspLI GGNNCC 5 cut(s) 298, 579, 951, 1401, 1634
BspMAI CTGCAG 1 cut(s) 796
BspMI ACCTGC 1 cut(s) 284
BspPI GGATC 5 cut(s) 944, 957, 1627, 1640, 1873
BspTNI GGTCTC 1 cut(s) 1367
BsrBI CCGCTC 1 cut(s) 198
BsrDI GCAATG 2 cut(s) 267, 708
BsrGI TGTACA 1 cut(s) 1802
BsrI ACTGG 5 cut(s) 197, 1086, 1617, 1630, 1749
BssECI CCNNGG 4 cut(s) 277, 1221, 1521, 1636
BssMI GATC 4 cut(s) 949, 1632, 1675, 1878
BssNAI GTATAC 1 cut(s) 459
BssSI CACGAG 1 cut(s) 572
BssT1I CCWWGG 2 cut(s) 277, 1221
Bst1107I GTATAC 1 cut(s) 459
Bst2BI CACGAG 1 cut(s) 572
Bst2UI CCWGG 1 cut(s) 1010
Bst4CI ACNGT 2 cut(s) 1335, 1802
Bst6I CTCTTC 4 cut(s) 381, 1103, 1300, 1817
BstAUI TGTACA 1 cut(s) 1802
BstC8I GCNNGC 3 cut(s) 291, 533, 996
BstDEI CTNAG 7 cut(s) 152, 476, 542, 1022, 1245, 1575, 1830
BstENI CCTNNNNNAGG 1 cut(s) 1613
BstF5I GGATG 5 cut(s) 514, 1018, 1437, 1738, 1762
BstFNI CGCG 2 cut(s) 140, 1510
BstHHI GCGC 5 cut(s) 26, 142, 715, 802, 1795
BstKTI GATC 4 cut(s) 952, 1635, 1678, 1881
BstMAI GTCTC 6 cut(s) 129, 656, 1105, 1258, 1367, 1578
BstMBI GATC 4 cut(s) 949, 1632, 1675, 1878
BstMCI CGRYCG 1 cut(s) 1264
BstMWI GCNNNNNNNGC 6 cut(s) 148, 239, 490, 710, 785, 794
BstNI CCWGG 1 cut(s) 1010
BstNSI RCATGY 1 cut(s) 1774
BstSCI CCNGG 4 cut(s) 1008, 1635, 1636, 1651
BstSFI CTRYAG 1 cut(s) 792
BstSLI GKGCMC 1 cut(s) 258
BstUI CGCG 2 cut(s) 140, 1510
BstV1I GCAGC 6 cut(s) 234, 588, 784, 806, 896, 1010
BstX2I RGATCY 3 cut(s) 949, 1632, 1878
BstYI RGATCY 3 cut(s) 949, 1632, 1878
BstZ17I GTATAC 1 cut(s) 459
BstZI CGGCCG 1 cut(s) 1261
BsuRI GGCC 5 cut(s) 372, 531, 704, 1263, 1828
BtsCI GGATG 5 cut(s) 514, 1018, 1437, 1738, 1762
BtsI GCAGTG 1 cut(s) 789
BtsIMutI CAGTG 2 cut(s) 789, 1079
BtuMI TCGCGA 1 cut(s) 1510
BveI ACCTGC 1 cut(s) 284
Cac8I GCNNGC 3 cut(s) 291, 533, 996
CciNI GCGGCCGC 1 cut(s) 1261
CfoI GCGC 5 cut(s) 26, 142, 715, 802, 1795
Cfr13I GGNCC 5 cut(s) 200, 371, 578, 1399, 1837
Cfr9I CCCGGG 1 cut(s) 1636
CpoI CGGWCCG 1 cut(s) 200
CseI GACGC 1 cut(s) 146
Csp6I GTAC 3 cut(s) 1196, 1430, 1803
CspI CGGWCCG 1 cut(s) 200
CviAII CATG 8 cut(s) 185, 669, 1123, 1184, 1286, 1406, 1771, 1810
CviQI GTAC 3 cut(s) 1196, 1430, 1803
DdeI CTNAG 7 cut(s) 152, 476, 542, 1022, 1245, 1575, 1830
DpnI GATC 4 cut(s) 951, 1634, 1677, 1880
DpnII GATC 4 cut(s) 949, 1632, 1675, 1878
DriI GACNNNNNGTC 1 cut(s) 669
EaeI YGGCCR 3 cut(s) 529, 702, 1261
EagI CGGCCG 1 cut(s) 1261
Eam1104I CTCTTC 4 cut(s) 381, 1103, 1300, 1817
Eam1105I GACNNNNNGTC 1 cut(s) 669
EarI CTCTTC 4 cut(s) 381, 1103, 1300, 1817
EciI GGCGGA 1 cut(s) 192
EclXI CGGCCG 1 cut(s) 1261
Eco130I CCWWGG 2 cut(s) 277, 1221
Eco147I AGGCCT 1 cut(s) 1828
Eco31I GGTCTC 1 cut(s) 1367
Eco47I GGWCC 4 cut(s) 200, 578, 1399, 1837
Eco52I CGGCCG 1 cut(s) 1261
Eco57I CTGAAG 1 cut(s) 87
Eco88I CYCGRG 1 cut(s) 1636
EcoNI CCTNNNNNAGG 1 cut(s) 1613
EcoO109I RGGNCCY 1 cut(s) 1399
EcoRI GAATTC 1 cut(s) 651
EcoRII CCWGG 1 cut(s) 1008
EcoT14I CCWWGG 2 cut(s) 277, 1221
ErhI CCWWGG 2 cut(s) 277, 1221
Esp3I CGTCTC 2 cut(s) 129, 1258
FaeI CATG 8 cut(s) 188, 672, 1126, 1187, 1289, 1409, 1774, 1813
FalI AAGNNNNNCTT 4 cut(s) 399, 431, 1297, 1329
FaqI GGGAC 1 cut(s) 1385
FatI CATG 8 cut(s) 184, 668, 1122, 1183, 1285, 1405, 1770, 1809
FauI CCCGC 1 cut(s) 316
FblI GTMKAC 2 cut(s) 458, 1718
FokI GGATG 5 cut(s) 521, 1025, 1424, 1745, 1769
FspBI CTAG 3 cut(s) 677, 1169, 1796
GlaI GCGC 5 cut(s) 25, 141, 714, 801, 1794
GsaI CCCAGC 1 cut(s) 1156
GsuI CTGGAG 2 cut(s) 214, 1634
HaeIII GGCC 5 cut(s) 372, 531, 704, 1263, 1828
HapII CCGG 4 cut(s) 369, 701, 1637, 1652
HgaI GACGC 1 cut(s) 146
HhaI GCGC 5 cut(s) 26, 142, 715, 802, 1795
Hin1II CATG 8 cut(s) 188, 672, 1126, 1187, 1289, 1409, 1774, 1813
Hin6I GCGC 5 cut(s) 24, 140, 713, 800, 1793
HinP1I GCGC 5 cut(s) 24, 140, 713, 800, 1793
HindIII AAGCTT 1 cut(s) 1472
HinfI GANTC 8 cut(s) 4, 64, 158, 282, 549, 1085, 1201, 1488
HpaII CCGG 4 cut(s) 369, 701, 1637, 1652
HphI GGTGA 4 cut(s) 1367, 1536, 1652, 1714
Hpy166II GTNNAC 6 cut(s) 265, 400, 459, 674, 1705, 1719
Hpy188I TCNGA 5 cut(s) 106, 479, 1522, 1578, 1684
Hpy188III TCNNGA 5 cut(s) 953, 1105, 1509, 1730, 1876
Hpy8I GTNNAC 6 cut(s) 265, 400, 459, 674, 1705, 1719
HpyAV CCTTC 5 cut(s) 311, 732, 1382, 1398, 1623
HpyCH4III ACNGT 2 cut(s) 1335, 1802
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 8 cut(s) 184, 210, 493, 604, 794, 853, 1034, 1162
HpyF10VI GCNNNNNNNGC 6 cut(s) 148, 239, 490, 710, 785, 794
HpyF3I CTNAG 7 cut(s) 152, 476, 542, 1022, 1245, 1575, 1830
HpySE526I ACGT 1 cut(s) 258
Hsp92II CATG 8 cut(s) 188, 672, 1126, 1187, 1289, 1409, 1774, 1813
HspAI GCGC 5 cut(s) 24, 140, 713, 800, 1793
Kzo9I GATC 4 cut(s) 949, 1632, 1675, 1878
LmnI GCTCC 2 cut(s) 195, 626
Lsp1109I GCAGC 6 cut(s) 234, 588, 784, 806, 896, 1010
LweI GCATC 6 cut(s) 33, 219, 840, 981, 1037, 1149
MaeI CTAG 3 cut(s) 677, 1169, 1796
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 3 cut(s) 91, 1542, 1621
MalI GATC 4 cut(s) 951, 1634, 1677, 1880
MbiI CCGCTC 1 cut(s) 198
MboI GATC 4 cut(s) 949, 1632, 1675, 1878
MfeI CAATTG 1 cut(s) 1163
MflI RGATCY 3 cut(s) 949, 1632, 1878
MhlI GDGCHC 1 cut(s) 258
MlsI TGGCCA 1 cut(s) 531
MluNI TGGCCA 1 cut(s) 531
MmeI TCCRAC 1 cut(s) 1465
Mox20I TGGCCA 1 cut(s) 531
MscI TGGCCA 1 cut(s) 531
MseI TTAA 3 cut(s) 71, 1116, 1866
Msp20I TGGCCA 1 cut(s) 531
MspA1I CMGCKG 3 cut(s) 80, 289, 797
MspI CCGG 4 cut(s) 369, 701, 1637, 1652
MspR9I CCNGG 4 cut(s) 1010, 1637, 1638, 1653
MunI CAATTG 1 cut(s) 1163
MvaI CCWGG 1 cut(s) 1010
MvnI CGCG 2 cut(s) 140, 1510
MwoI GCNNNNNNNGC 6 cut(s) 148, 239, 490, 710, 785, 794
NciI CCSGG 3 cut(s) 1637, 1638, 1653
NdeII GATC 4 cut(s) 949, 1632, 1675, 1878
NlaIII CATG 8 cut(s) 188, 672, 1126, 1187, 1289, 1409, 1774, 1813
NlaIV GGNNCC 5 cut(s) 298, 579, 951, 1401, 1634
NmuCI GTSAC 1 cut(s) 1542
NotI GCGGCCGC 1 cut(s) 1261
NruI TCGCGA 1 cut(s) 1510
NspI RCATGY 1 cut(s) 1774
PceI AGGCCT 1 cut(s) 1828
PciI ACATGT 1 cut(s) 1770
PfeI GAWTC 8 cut(s) 4, 64, 158, 282, 549, 1085, 1201, 1488
PflMI CCANNNNNTGG 1 cut(s) 527
PfoI TCCNGGA 1 cut(s) 1651
PpuMI RGGWCCY 1 cut(s) 1399
PscI ACATGT 1 cut(s) 1770
PshBI ATTAAT 1 cut(s) 1866
PsiI TTATAA 1 cut(s) 564
Psp5II RGGWCCY 1 cut(s) 1399
Psp6I CCWGG 1 cut(s) 1008
PspFI CCCAGC 1 cut(s) 1152
PspGI CCWGG 1 cut(s) 1008
PspN4I GGNNCC 5 cut(s) 298, 579, 951, 1401, 1634
PspPI GGNCC 5 cut(s) 200, 371, 578, 1399, 1837
PspPPI RGGWCCY 1 cut(s) 1399
PsrI GAACNNNNNNTAC 2 cut(s) 229, 261
PstI CTGCAG 1 cut(s) 796
PsuI RGATCY 3 cut(s) 949, 1632, 1878
PvuII CAGCTG 3 cut(s) 80, 289, 797
RruI TCGCGA 1 cut(s) 1510
RsaI GTAC 3 cut(s) 1197, 1431, 1804
RsaNI GTAC 3 cut(s) 1196, 1430, 1803
Rsr2I CGGWCCG 1 cut(s) 200
RsrII CGGWCCG 1 cut(s) 200
SaqAI TTAA 3 cut(s) 71, 1116, 1866
Sau3AI GATC 4 cut(s) 949, 1632, 1675, 1878
Sau96I GGNCC 5 cut(s) 200, 371, 578, 1399, 1837
ScrFI CCNGG 4 cut(s) 1010, 1637, 1638, 1653
SduI GDGCHC 1 cut(s) 258
SfaNI GCATC 6 cut(s) 33, 219, 840, 981, 1037, 1149
SfcI CTRYAG 1 cut(s) 792
SinI GGWCC 4 cut(s) 200, 578, 1399, 1837
SmaI CCCGGG 1 cut(s) 1638
SmlI CTYRAG 3 cut(s) 485, 1557, 1874
SmoI CTYRAG 3 cut(s) 485, 1557, 1874
SpeI ACTAGT 1 cut(s) 676
SseBI AGGCCT 1 cut(s) 1828
SsiI CCGC 7 cut(s) 198, 203, 309, 866, 1260, 1264, 1748
SspI AATATT 1 cut(s) 733
SspMI CTAG 3 cut(s) 677, 1169, 1796
StuI AGGCCT 1 cut(s) 1828
StyD4I CCNGG 4 cut(s) 1008, 1635, 1636, 1651
StyI CCWWGG 2 cut(s) 277, 1221
TaaI ACNGT 2 cut(s) 1335, 1802
TaiI ACGT 1 cut(s) 261
TaqI TCGA 2 cut(s) 170, 1337
TatI WGTACW 2 cut(s) 1429, 1802
TauI GCSGC 4 cut(s) 869, 1263, 1266, 1751
TfiI GAWTC 8 cut(s) 4, 64, 158, 282, 549, 1085, 1201, 1488
Tru1I TTAA 3 cut(s) 71, 1116, 1866
Tru9I TTAA 3 cut(s) 71, 1116, 1866
TscAI CASTG 2 cut(s) 796, 1086
TseFI GTSAC 1 cut(s) 1542
TseI GCWGC 6 cut(s) 222, 601, 794, 797, 884, 998
Tsp45I GTSAC 1 cut(s) 1542
TspDTI ATGAA 5 cut(s) 17, 129, 1139, 1200, 1677
TspGWI ACGGA 2 cut(s) 151, 1213
TspMI CCCGGG 1 cut(s) 1636
TspRI CASTG 2 cut(s) 796, 1086
Van91I CCANNNNNTGG 1 cut(s) 527
VpaK11BI GGWCC 4 cut(s) 200, 578, 1399, 1837
VspI ATTAAT 1 cut(s) 1866
XagI CCTNNNNNAGG 1 cut(s) 1613
XapI RAATTY 5 cut(s) 85, 516, 608, 651, 1145
XceI RCATGY 1 cut(s) 1774
XmaI CCCGGG 1 cut(s) 1636
XmiI GTMKAC 2 cut(s) 458, 1718
XspI CTAG 3 cut(s) 677, 1169, 1796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.