Rmu_co8477461.1_g000001

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8477461.1
OG0016852
Physical Location & Seq
Reverse (-)
321 .. 2065
1745 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8477461.1_g000001.1.cds

Sequence Viewer

Length: 1377 bp
ctgagcaaacttagcttcagaaacaacaccttctctggctctcttccaaacctatcaaatcttgttcacctggagtatgtggatttctcatacaaccgtttctggggttccatacctttcgagtacactactgatttgccaaagctggaactgctagagttgcaagagaattctttagatgggtcaattccgcctttcgaccaaccaacactcagagcttttaatgtttctcataatcatctcgacggtccaattcctgacactggtgtgttacagaagttccccaaaagctcgtacgatcataatgcaaatctttgtgggagtcctctggggatatcttgtactcctcctccatctcctaattccccgccaaacgattcaaaagatgacctcccactcgctttgattgtggctgcagcagcggcggttcctttttttgttatatttgtattcttatgttattataagaaagtgcatggaaaagatgaagcgacgtcaacagatcaggccgagcagacggagtgggcagtggcacaaaagaaaatgcctaatgatgatcatttggatgagaggttgggggatcctgaaaagaaggtcgagttggaatttttcgacaaggacttgcccgccgcggcatttttcgatttggatgatttacttcgggcttcagccgaagtgttaggcaggggaaaactgggaacttcatacaaagtaacactagaatcgggccctgtcgtggtggtgaagagactaagaagcatgaacgagttgagcaaaaaggaatttgtccagcaaatgcaattgctagggaagatgagacacgaaaaccttgtacagattctctccttctactattccaaggaggagaagctcgtcatctataagtttgtctctggtgccactactttgtttgagcttttacatgttgttatccgagacgaaaactatcatgtgaagctcacagatttcggattcttgccactcctgcctgctcgagaatgctcatcacaaaatagcattctagcaattggtaagtcaccggagttggctcaagcgaagaagttgtcacagaaagccgatgtctattgctttggcattgtcctactggaggtcattactggcaggatcccaggcaaaatctcaccgggaagtgatgaaacaattgatgatctttccgattgggttagaatggtggtaaacaatgattggtccacagatattttggatgtagaaatgctggcagcaaaagatgaccacgatgacatgttgaagctgaccaacattgccctagagtgtacagacatcgccccagagaaccggcctaaaatgattcaagtattgaaaagcattcaagaaatagagagtagttcaagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 Pkinase Pkinase_Tyr

Protein Analysis

458

Amino Acids

51.29

Weight (kDa)

5.01

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016852)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g18620
malus_domestica MD14G1247600.v1.1
prunus_persica Prupe.5G246000_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0176881
rosa_laevigata RLG00000005576
rosa_multiflora Rmu_co8477461.1_g000001 Rmu_sc0005310.1_g000052
rosa_roxburghii Rroxscaffold_3G00276320
rosa_rugosa Rorug06G0401300
rosa_samantha Rh7AG000800 Rh7BG001400 Rh7CG000800 Rh7DG001100
rosa_wichuraiana Rw7G000070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 465
AatII GACGTC 1 cut(s) 497
AccB1I GGYRCC 1 cut(s) 896
AccII CGCG 1 cut(s) 632
AciI CCGC 7 cut(s) 191, 368, 422, 425, 627, 630, 632
AclWI GGATC 4 cut(s) 575, 588, 1120, 1133
AcsI RAATTY 3 cut(s) 169, 605, 782
AcuI CTGAAG 1 cut(s) 651
AcyI GRCGYC 1 cut(s) 494
AfaI GTAC 5 cut(s) 125, 296, 343, 834, 1297
AfiI CCNNNNNNNGG 4 cut(s) 103, 263, 736, 1108
AflIII ACRYGT 2 cut(s) 922, 1263
AgsI TTSAA 6 cut(s) 381, 1270, 1334, 1342, 1352, 1371
AjnI CCWGG 2 cut(s) 69, 1129
AjuI GAANNNNNNNTTGG 2 cut(s) 584, 616
AleI CACNNNNGTG 1 cut(s) 266
AluBI AGCT 8 cut(s) 15, 145, 218, 291, 871, 916, 958, 1273
AluI AGCT 8 cut(s) 15, 145, 218, 291, 871, 916, 958, 1273
Alw26I GTCTC 4 cut(s) 742, 811, 895, 930
AlwI GGATC 4 cut(s) 575, 588, 1120, 1133
Ama87I CYCGRG 1 cut(s) 993
AoxI GGCC 3 cut(s) 507, 727, 1319
ApaI GGGCCC 1 cut(s) 731
ApeKI GCWGC 4 cut(s) 413, 416, 419, 1241
ApoI RAATTY 3 cut(s) 169, 605, 782
ArsI GACNNNNNNTTYG 2 cut(s) 1049, 1081
AspS9I GGNCC 4 cut(s) 248, 727, 728, 1209
AsuC2I CCSGG 1 cut(s) 1146
AsuHPI GGTGA 4 cut(s) 59, 754, 1029, 1134
AvaI CYCGRG 1 cut(s) 993
AvaII GGWCC 2 cut(s) 248, 1209
BaeGI GKGCMC 1 cut(s) 731
BamHI GGATCC 2 cut(s) 580, 1125
BanI GGYRCC 1 cut(s) 896
BanII GRGCYC 1 cut(s) 731
BbvI GCAGC 4 cut(s) 400, 428, 431, 1253
BccI CCATC 2 cut(s) 173, 361
BcgI CGANNNNNNTGC 2 cut(s) 287, 321
BciT130I CCWGG 2 cut(s) 71, 1131
BclI TGATCA 1 cut(s) 556
BcnI CCSGG 1 cut(s) 1146
BcoDI GTCTC 4 cut(s) 742, 811, 895, 930
BfaI CTAG 5 cut(s) 155, 719, 806, 1022, 1289
BfmI CTRYAG 1 cut(s) 414
BisI GCNGC 7 cut(s) 414, 417, 420, 423, 630, 633, 1242
BlsI GCNGC 7 cut(s) 415, 418, 421, 424, 631, 634, 1243
Bme1390I CCNGG 3 cut(s) 71, 1131, 1146
Bme18I GGWCC 2 cut(s) 248, 1209
BmeT110I CYCGRG 1 cut(s) 993
BmgT120I GGNCC 4 cut(s) 248, 727, 728, 1209
BmiI GGNNCC 6 cut(s) 109, 429, 582, 729, 898, 1127
BmrFI CCNGG 3 cut(s) 71, 1131, 1146
BmrI ACTGGG 1 cut(s) 704
BmuI ACTGGG 1 cut(s) 704
BpmI CTGGAG 2 cut(s) 92, 1127
BpuEI CTTGAG 1 cut(s) 1035
BpuMI CCSGG 1 cut(s) 1146
BsaHI GRCGYC 1 cut(s) 494
BsaJI CCNNGG 3 cut(s) 630, 858, 1129
BsaWI WCCGGW 1 cut(s) 1039
BsaXI ACNNNNNCTCC 2 cut(s) 334, 364
Bsc4I CCNNNNNNNGG 4 cut(s) 103, 263, 736, 1108
Bse118I RCCGGY 1 cut(s) 1317
Bse1I ACTGG 4 cut(s) 268, 699, 1110, 1123
Bse3DI GCAATG 1 cut(s) 1281
BseBI CCWGG 2 cut(s) 71, 1131
BseDI CCNNGG 3 cut(s) 630, 858, 1129
BseGI GGATG 3 cut(s) 571, 655, 1231
BseLI CCNNNNNNNGG 4 cut(s) 103, 263, 736, 1108
BseMI GCAATG 1 cut(s) 1281
BseMII CTCAG 1 cut(s) 226
BseNI ACTGG 4 cut(s) 268, 699, 1110, 1123
BseRI GAGGAG 3 cut(s) 336, 339, 878
BseSI GKGCMC 1 cut(s) 731
BseXI GCAGC 4 cut(s) 400, 428, 431, 1253
Bsh1236I CGCG 1 cut(s) 632
BshFI GGCC 3 cut(s) 509, 729, 1321
BshNI GGYRCC 1 cut(s) 896
BsiHKCI CYCGRG 1 cut(s) 993
BsiSI CCGG 3 cut(s) 1040, 1145, 1318
BsiWI CGTACG 1 cut(s) 294
BslI CCNNNNNNNGG 4 cut(s) 103, 263, 736, 1108
BsmAI GTCTC 4 cut(s) 742, 811, 895, 930
BsmBI CGTCTC 1 cut(s) 930
BsmI GAATGC 3 cut(s) 1004, 1017, 1347
BsnI GGCC 3 cut(s) 509, 729, 1321
BsoBI CYCGRG 1 cut(s) 993
Bsp120I GGGCCC 1 cut(s) 727
Bsp1286I GDGCHC 1 cut(s) 731
Bsp1407I TGTACA 2 cut(s) 832, 1295
Bsp143I GATC 6 cut(s) 298, 502, 556, 580, 1125, 1168
BspACI CCGC 7 cut(s) 191, 368, 422, 425, 627, 630, 632
BspANI GGCC 3 cut(s) 509, 729, 1321
BspCNI CTCAG 1 cut(s) 225
BspFNI CGCG 1 cut(s) 632
BspLI GGNNCC 6 cut(s) 109, 429, 582, 729, 898, 1127
BspMAI CTGCAG 1 cut(s) 418
BspPI GGATC 4 cut(s) 575, 588, 1120, 1133
BspT107I GGYRCC 1 cut(s) 896
BsrDI GCAATG 1 cut(s) 1281
BsrFI RCCGGY 1 cut(s) 1317
BsrGI TGTACA 2 cut(s) 832, 1295
BsrI ACTGG 4 cut(s) 268, 699, 1110, 1123
BssAI RCCGGY 1 cut(s) 1317
BssECI CCNNGG 3 cut(s) 630, 858, 1129
BssMI GATC 6 cut(s) 298, 502, 556, 580, 1125, 1168
BssNI GRCGYC 1 cut(s) 494
BssT1I CCWWGG 1 cut(s) 858
Bst2UI CCWGG 2 cut(s) 71, 1131
Bst4CI ACNGT 2 cut(s) 98, 248
Bst6I CTCTTC 2 cut(s) 48, 740
BstACI GRCGYC 1 cut(s) 494
BstAUI TGTACA 2 cut(s) 832, 1295
BstC8I GCNNGC 3 cut(s) 627, 990, 1239
BstDEI CTNAG 4 cut(s) 2, 11, 212, 752
BstDSI CCRYGG 1 cut(s) 630
BstENI CCTNNNNNAGG 1 cut(s) 1106
BstF5I GGATG 3 cut(s) 571, 655, 1231
BstFNI CGCG 1 cut(s) 632
BstKTI GATC 6 cut(s) 301, 505, 559, 583, 1128, 1171
BstMAI GTCTC 4 cut(s) 742, 811, 895, 930
BstMBI GATC 6 cut(s) 298, 502, 556, 580, 1125, 1168
BstMWI GCNNNNNNNGC 6 cut(s) 12, 151, 160, 419, 422, 985
BstNI CCWGG 2 cut(s) 71, 1131
BstNSI RCATGY 2 cut(s) 926, 1267
BstSCI CCNGG 3 cut(s) 69, 1129, 1144
BstSFI CTRYAG 1 cut(s) 414
BstSLI GKGCMC 1 cut(s) 731
BstUI CGCG 1 cut(s) 632
BstV1I GCAGC 4 cut(s) 400, 428, 431, 1253
BstX2I RGATCY 2 cut(s) 580, 1125
BstYI RGATCY 2 cut(s) 580, 1125
BsuRI GGCC 3 cut(s) 509, 729, 1321
BtgI CCRYGG 1 cut(s) 630
BtgZI GCGATG 1 cut(s) 1288
BtsCI GGATG 3 cut(s) 571, 655, 1231
BtsI GCAGTG 1 cut(s) 534
BtsIMutI CAGTG 2 cut(s) 261, 534
Cac8I GCNNGC 3 cut(s) 627, 990, 1239
Cfr10I RCCGGY 1 cut(s) 1317
Cfr13I GGNCC 4 cut(s) 248, 727, 728, 1209
Cfr42I CCGCGG 1 cut(s) 633
Csp6I GTAC 5 cut(s) 124, 295, 342, 833, 1296
CspCI CAANNNNNGTGG 2 cut(s) 384, 419
CviAII CATG 5 cut(s) 476, 760, 923, 950, 1264
CviQI GTAC 5 cut(s) 124, 295, 342, 833, 1296
DdeI CTNAG 4 cut(s) 2, 11, 212, 752
DpnI GATC 6 cut(s) 300, 504, 558, 582, 1127, 1170
DpnII GATC 6 cut(s) 298, 502, 556, 580, 1125, 1168
Eam1104I CTCTTC 2 cut(s) 48, 740
EarI CTCTTC 2 cut(s) 48, 740
EciI GGCGGA 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 858
Eco24I GRGCYC 1 cut(s) 731
Eco32I GATATC 1 cut(s) 336
Eco47I GGWCC 2 cut(s) 248, 1209
Eco57I CTGAAG 1 cut(s) 651
Eco88I CYCGRG 1 cut(s) 993
EcoNI CCTNNNNNAGG 1 cut(s) 1106
EcoO109I RGGNCCY 1 cut(s) 728
EcoRI GAATTC 1 cut(s) 169
EcoRII CCWGG 2 cut(s) 69, 1129
EcoRV GATATC 1 cut(s) 336
EcoT14I CCWWGG 1 cut(s) 858
EcoT38I GRGCYC 1 cut(s) 731
ErhI CCWWGG 1 cut(s) 858
Esp3I CGTCTC 1 cut(s) 930
FaeI CATG 5 cut(s) 479, 763, 926, 953, 1267
FatI CATG 5 cut(s) 475, 759, 922, 949, 1263
FauI CCCGC 2 cut(s) 375, 634
FbaI TGATCA 1 cut(s) 556
Fnu4HI GCNGC 7 cut(s) 414, 417, 420, 423, 630, 633, 1242
FokI GGATG 3 cut(s) 578, 662, 1238
FriOI GRGCYC 1 cut(s) 731
Fsp4HI GCNGC 7 cut(s) 414, 417, 420, 423, 630, 633, 1242
FspBI CTAG 5 cut(s) 155, 719, 806, 1022, 1289
GluI GCNGC 7 cut(s) 414, 417, 420, 423, 630, 633, 1242
GsuI CTGGAG 2 cut(s) 92, 1127
HaeIII GGCC 3 cut(s) 509, 729, 1321
HapII CCGG 3 cut(s) 1040, 1145, 1318
Hin1I GRCGYC 1 cut(s) 494
Hin1II CATG 5 cut(s) 479, 763, 926, 953, 1267
HincII GTYRAC 1 cut(s) 498
HindII GTYRAC 1 cut(s) 498
HinfI GANTC 6 cut(s) 322, 377, 722, 838, 972, 1330
HpaII CCGG 3 cut(s) 1040, 1145, 1318
HphI GGTGA 4 cut(s) 59, 754, 1029, 1134
Hpy166II GTNNAC 6 cut(s) 67, 126, 498, 1198, 1212, 1296
Hpy188I TCNGA 5 cut(s) 20, 215, 935, 971, 1177
Hpy188III TCNNGA 6 cut(s) 242, 257, 584, 995, 1352, 1371
Hpy8I GTNNAC 6 cut(s) 67, 126, 498, 1198, 1212, 1296
Hpy99I CGWCG 2 cut(s) 248, 496
HpyAV CCTTC 3 cut(s) 40, 586, 856
HpyCH4III ACNGT 2 cut(s) 98, 248
HpyCH4IV ACGT 1 cut(s) 494
HpyCH4V TGCA 5 cut(s) 163, 308, 416, 475, 799
HpyF10VI GCNNNNNNNGC 6 cut(s) 12, 151, 160, 419, 422, 985
HpyF3I CTNAG 4 cut(s) 2, 11, 212, 752
HpySE526I ACGT 1 cut(s) 494
Hsp92I GRCGYC 1 cut(s) 494
Hsp92II CATG 5 cut(s) 479, 763, 926, 953, 1267
Ksp22I TGATCA 1 cut(s) 556
KspI CCGCGG 1 cut(s) 633
Kzo9I GATC 6 cut(s) 298, 502, 556, 580, 1125, 1168
Lsp1109I GCAGC 4 cut(s) 400, 428, 431, 1253
MaeI CTAG 5 cut(s) 155, 719, 806, 1022, 1289
MaeII ACGT 1 cut(s) 494
MaeIII GTNAC 4 cut(s) 270, 712, 1035, 1065
MalI GATC 6 cut(s) 300, 504, 558, 582, 1127, 1170
MboI GATC 6 cut(s) 298, 502, 556, 580, 1125, 1168
MboII GAAGA 4 cut(s) 35, 757, 823, 1069
MfeI CAATTG 3 cut(s) 800, 1026, 1161
MflI RGATCY 2 cut(s) 580, 1125
MhlI GDGCHC 1 cut(s) 731
MluCI AATT 9 cut(s) 169, 186, 252, 361, 605, 782, 800, 1026, 1161
MlyI GAGTC 1 cut(s) 331
MmeI TCCRAC 1 cut(s) 582
MnlI CCTC 7 cut(s) 336, 357, 360, 401, 564, 856, 1102
MseI TTAA 1 cut(s) 222
MslI CAYNNNNRTG 2 cut(s) 266, 564
MspA1I CMGCKG 2 cut(s) 422, 632
MspI CCGG 3 cut(s) 1040, 1145, 1318
MspR9I CCNGG 3 cut(s) 71, 1131, 1146
MunI CAATTG 3 cut(s) 800, 1026, 1161
Mva1269I GAATGC 3 cut(s) 1004, 1017, 1347
MvaI CCWGG 2 cut(s) 71, 1131
MvnI CGCG 1 cut(s) 632
MwoI GCNNNNNNNGC 6 cut(s) 12, 151, 160, 419, 422, 985
NciI CCSGG 1 cut(s) 1146
NdeII GATC 6 cut(s) 298, 502, 556, 580, 1125, 1168
NlaIII CATG 5 cut(s) 479, 763, 926, 953, 1267
NlaIV GGNNCC 6 cut(s) 109, 429, 582, 729, 898, 1127
NmeAIII GCCGAG 1 cut(s) 535
NmuCI GTSAC 2 cut(s) 1035, 1065
NspI RCATGY 2 cut(s) 926, 1267
OliI CACNNNNGTG 1 cut(s) 266
PaeR7I CTCGAG 1 cut(s) 993
PciI ACATGT 2 cut(s) 922, 1263
PctI GAATGC 3 cut(s) 1004, 1017, 1347
PfeI GAWTC 5 cut(s) 377, 722, 838, 972, 1330
Pfl23II CGTACG 1 cut(s) 294
PkrI GCNGC 7 cut(s) 415, 418, 421, 424, 631, 634, 1243
PleI GAGTC 1 cut(s) 330
PpsI GAGTC 1 cut(s) 330
PscI ACATGT 2 cut(s) 922, 1263
PsiI TTATAA 1 cut(s) 465
Psp6I CCWGG 2 cut(s) 69, 1129
PspGI CCWGG 2 cut(s) 69, 1129
PspLI CGTACG 1 cut(s) 294
PspN4I GGNNCC 6 cut(s) 109, 429, 582, 729, 898, 1127
PspOMI GGGCCC 1 cut(s) 727
PspPI GGNCC 4 cut(s) 248, 727, 728, 1209
PstI CTGCAG 1 cut(s) 418
PsuI RGATCY 2 cut(s) 580, 1125
RsaI GTAC 5 cut(s) 125, 296, 343, 834, 1297
RsaNI GTAC 5 cut(s) 124, 295, 342, 833, 1296
RseI CAYNNNNRTG 2 cut(s) 266, 564
SacII CCGCGG 1 cut(s) 633
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 7 cut(s) 414, 417, 420, 423, 630, 633, 1242
Sau3AI GATC 6 cut(s) 298, 502, 556, 580, 1125, 1168
Sau96I GGNCC 4 cut(s) 248, 727, 728, 1209
SchI GAGTC 1 cut(s) 331
ScrFI CCNGG 3 cut(s) 71, 1131, 1146
SduI GDGCHC 1 cut(s) 731
SfcI CTRYAG 1 cut(s) 414
Sfr274I CTCGAG 1 cut(s) 993
Sfr303I CCGCGG 1 cut(s) 633
SgrBI CCGCGG 1 cut(s) 633
SinI GGWCC 2 cut(s) 248, 1209
SlaI CTCGAG 1 cut(s) 993
SmiMI CAYNNNNRTG 2 cut(s) 266, 564
SmlI CTYRAG 2 cut(s) 993, 1050
SmoI CTYRAG 2 cut(s) 993, 1050
Sse9I AATT 9 cut(s) 169, 186, 252, 361, 605, 782, 800, 1026, 1161
SsiI CCGC 7 cut(s) 191, 368, 422, 425, 627, 630, 632
SspMI CTAG 5 cut(s) 155, 719, 806, 1022, 1289
StyD4I CCNGG 3 cut(s) 69, 1129, 1144
StyI CCWWGG 1 cut(s) 858
TaaI ACNGT 2 cut(s) 98, 248
TaiI ACGT 1 cut(s) 497
TaqI TCGA 7 cut(s) 120, 198, 243, 597, 612, 642, 994
TasI AATT 9 cut(s) 169, 186, 252, 361, 605, 782, 800, 1026, 1161
TatI WGTACW 4 cut(s) 123, 341, 832, 1295
TauI GCSGC 3 cut(s) 425, 632, 635
TfiI GAWTC 5 cut(s) 377, 722, 838, 972, 1330
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 2 cut(s) 268, 534
TseFI GTSAC 2 cut(s) 1035, 1065
TseI GCWGC 4 cut(s) 413, 416, 419, 1241
Tsp45I GTSAC 2 cut(s) 1035, 1065
TspDTI ATGAA 4 cut(s) 501, 693, 776, 1170
TspGWI ACGGA 1 cut(s) 533
TspRI CASTG 2 cut(s) 268, 534
VpaK11BI GGWCC 2 cut(s) 248, 1209
XagI CCTNNNNNAGG 1 cut(s) 1106
XapI RAATTY 3 cut(s) 169, 605, 782
XceI RCATGY 2 cut(s) 926, 1267
XcmI CCANNNNNNNNNTGG 1 cut(s) 1219
XhoI CTCGAG 1 cut(s) 993
XspI CTAG 5 cut(s) 155, 719, 806, 1022, 1289
ZraI GACGTC 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.