FvH4_5g21120

Belongs to the expansin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0018044
Physical Location & Seq
Forward (+)
12694528 .. 12696636
2109 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g21120.t3

Sequence Viewer

Length: 774 bp
ATGGCTTTCTGTCTGCGATCACCTTTGTGCCTTGTCATCGTTTTTCTTTTCTTACAAACACTGGCCGGTAAGGCTGCAACGTGTAATGACTGCTTTGTTCAATCTCGAGCAGCTTATTATCCAAATTCTGAAACACAAGGAACAGAAATCGGTGCTTGCGGGTTCGGCACATTCGGAGCAACAATCAATGGTGGGGATGTTTCAGCAGCCTCTGACCTCTTTCGCAATGGGGTTGGATGTGGTGCTTGTTACCAGGTGAGGTGCACAAATGGCGGCTACTGCTCAGACAAAGGAGTAACTGTAGTTATAACAGATTCAGGCTCTAGTGATCGCACAGACTTTATCCTCAGCCAACGAGCGTTTGCAAGGATGGCTCAAACAACTGATGCAGCTGCTTCTCTACTATCTCTTGGTATAGTTGATATCGAATACAGACGTGTTTCATGCAGCTACCCCAACAAAAATATAACAATCAAGATTGATGAGAACAGTAACTATCCTTATTATTTGGCCTTTGTAATATGGTACCAACAAGGAAAGAAAGATATCACAGCTGTCCAGCTATGTGAGACACAAAACTTTGTCTGTAAGTTACTGGACCGGAGCTATGGAGCAGTTTGGACAACTACCTCACCTCCAACTGGACCCCTGCAAATAAGGATGTTATTCAGTGATGATGACGGTGATGAGAATTGGGTTGTTCCTGTGAATAACATACCACAAGACTGGGAAGTTGGACAAACCTATGATTCGGGAGTTCAAGTGAACTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

258

Amino Acids

28.03

Weight (kDa)

4.59

Isoelectric Point (pI)

40.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 71 - 144 1.7e-13 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018044)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g21120
malus_domestica MD06G1041000.v1.1
prunus_persica Prupe.5G047300_v2.0.a1 Prupe.5G047300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0208241
rosa_laevigata RLG00000003236
rosa_multiflora Rmu_sc0007869.1_g000018
rosa_roxburghii Rroxscaffold_3G00250650
rosa_rugosa Rorug07G0102400
rosa_samantha Rh7CG252500
rosa_wichuraiana Rw7G020190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 308, 772
Acc65I GGTACC 1 cut(s) 525
AccB1I GGYRCC 1 cut(s) 525
AciI CCGC 2 cut(s) 159, 273
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 1 cut(s) 124
AfaI GTAC 1 cut(s) 527
AfiI CCNNNNNNNGG 1 cut(s) 641
AflIII ACRYGT 2 cut(s) 80, 436
AgsI TTSAA 2 cut(s) 101, 761
AjiI CACGTC 1 cut(s) 437
AjnI CCWGG 1 cut(s) 252
AleI CACNNNNGTG 1 cut(s) 25
AluBI AGCT 6 cut(s) 113, 392, 450, 554, 562, 606
AluI AGCT 6 cut(s) 113, 392, 450, 554, 562, 606
Alw21I GWGCWC 1 cut(s) 266
Alw26I GTCTC 1 cut(s) 563
Alw44I GTGCAC 1 cut(s) 262
AlwNI CAGNNNCTG 1 cut(s) 212
Ama87I CYCGRG 1 cut(s) 105
AoxI GGCC 2 cut(s) 63, 510
ApaLI GTGCAC 1 cut(s) 262
ApeKI GCWGC 6 cut(s) 74, 110, 206, 389, 392, 447
ApoI RAATTY 1 cut(s) 124
Asp718I GGTACC 1 cut(s) 525
AspS9I GGNCC 2 cut(s) 598, 644
AsuHPI GGTGA 4 cut(s) 12, 268, 624, 695
AvaI CYCGRG 1 cut(s) 105
AvaII GGWCC 2 cut(s) 598, 644
BaeGI GKGCMC 1 cut(s) 266
BanI GGYRCC 1 cut(s) 525
Bbv12I GWGCWC 1 cut(s) 266
BbvCI CCTCAGC 1 cut(s) 347
BbvI GCAGC 6 cut(s) 61, 122, 218, 379, 401, 459
BccI CCATC 1 cut(s) 364
BciT130I CCWGG 1 cut(s) 254
BcoDI GTCTC 1 cut(s) 563
BfaI CTAG 1 cut(s) 324
BfmI CTRYAG 1 cut(s) 300
BglI GCCNNNNNGGC 1 cut(s) 71
BisI GCNGC 7 cut(s) 75, 111, 207, 274, 390, 393, 448
BlsI GCNGC 7 cut(s) 76, 112, 208, 275, 391, 394, 449
Bme1390I CCNGG 1 cut(s) 254
Bme18I GGWCC 2 cut(s) 598, 644
BmeT110I CYCGRG 1 cut(s) 105
BmgBI CACGTC 1 cut(s) 437
BmgT120I GGNCC 2 cut(s) 598, 644
BmiI GGNNCC 2 cut(s) 527, 646
BmrFI CCNGG 1 cut(s) 254
BmrI ACTGGG 1 cut(s) 736
BmsI GCATC 1 cut(s) 376
BmuI ACTGGG 1 cut(s) 736
Bpu10I CCTNAGC 1 cut(s) 347
BsaWI WCCGGW 1 cut(s) 600
BsaXI ACNNNNNCTCC 5 cut(s) 285, 315, 619, 649, 747
Bsc4I CCNNNNNNNGG 1 cut(s) 641
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 4 cut(s) 66, 600, 646, 731
Bse3DI GCAATG 1 cut(s) 232
BseBI CCWGG 1 cut(s) 254
BseGI GGATG 4 cut(s) 202, 242, 375, 666
BseLI CCNNNNNNNGG 1 cut(s) 641
BseMI GCAATG 1 cut(s) 232
BseMII CTCAG 2 cut(s) 297, 361
BseNI ACTGG 4 cut(s) 66, 600, 646, 731
BseSI GKGCMC 1 cut(s) 266
BseXI GCAGC 6 cut(s) 61, 122, 218, 379, 401, 459
BshFI GGCC 2 cut(s) 65, 512
BshNI GGYRCC 1 cut(s) 525
BsiHKAI GWGCWC 1 cut(s) 266
BsiHKCI CYCGRG 1 cut(s) 105
BsiSI CCGG 2 cut(s) 66, 601
BslI CCNNNNNNNGG 1 cut(s) 641
BsmAI GTCTC 1 cut(s) 563
BsnI GGCC 2 cut(s) 65, 512
BsoBI CYCGRG 1 cut(s) 105
Bsp1286I GDGCHC 1 cut(s) 266
Bsp143I GATC 2 cut(s) 17, 328
BspACI CCGC 2 cut(s) 159, 273
BspANI GGCC 2 cut(s) 65, 512
BspCNI CTCAG 2 cut(s) 296, 360
BspLI GGNNCC 2 cut(s) 527, 646
BspT107I GGYRCC 1 cut(s) 525
BsrDI GCAATG 1 cut(s) 232
BsrFI RCCGGY 1 cut(s) 65
BsrI ACTGG 4 cut(s) 66, 600, 646, 731
BssAI RCCGGY 1 cut(s) 65
BssMI GATC 2 cut(s) 17, 328
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 3 cut(s) 301, 491, 683
BstC8I GCNNGC 1 cut(s) 157
BstDEI CTNAG 2 cut(s) 283, 347
BstF5I GGATG 4 cut(s) 202, 242, 375, 666
BstKTI GATC 2 cut(s) 20, 331
BstMAI GTCTC 1 cut(s) 563
BstMBI GATC 2 cut(s) 17, 328
BstMWI GCNNNNNNNGC 5 cut(s) 71, 165, 270, 279, 371
BstNI CCWGG 1 cut(s) 254
BstSCI CCNGG 1 cut(s) 252
BstSFI CTRYAG 1 cut(s) 300
BstSLI GKGCMC 1 cut(s) 266
BstV1I GCAGC 6 cut(s) 61, 122, 218, 379, 401, 459
BstXI CCANNNNNNTGG 1 cut(s) 726
BsuRI GGCC 2 cut(s) 65, 512
BtrI CACGTC 1 cut(s) 437
BtsCI GGATG 4 cut(s) 202, 242, 375, 666
BtsIMutI CAGTG 2 cut(s) 59, 676
Cac8I GCNNGC 1 cut(s) 157
CaiI CAGNNNCTG 1 cut(s) 212
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 2 cut(s) 598, 644
CsiI ACCWGGT 1 cut(s) 252
Csp6I GTAC 1 cut(s) 526
CviAII CATG 1 cut(s) 444
CviQI GTAC 1 cut(s) 526
DdeI CTNAG 2 cut(s) 283, 347
DpnI GATC 2 cut(s) 19, 330
DpnII GATC 2 cut(s) 17, 328
EaeI YGGCCR 1 cut(s) 63
Eco32I GATATC 2 cut(s) 424, 547
Eco47I GGWCC 2 cut(s) 598, 644
Eco88I CYCGRG 1 cut(s) 105
EcoRII CCWGG 1 cut(s) 252
EcoRV GATATC 2 cut(s) 424, 547
FaeI CATG 1 cut(s) 447
FatI CATG 1 cut(s) 443
FauI CCCGC 1 cut(s) 152
Fnu4HI GCNGC 7 cut(s) 75, 111, 207, 274, 390, 393, 448
FokI GGATG 4 cut(s) 209, 249, 382, 673
Fsp4HI GCNGC 7 cut(s) 75, 111, 207, 274, 390, 393, 448
FspBI CTAG 1 cut(s) 324
GluI GCNGC 7 cut(s) 75, 111, 207, 274, 390, 393, 448
HaeIII GGCC 2 cut(s) 65, 512
HapII CCGG 2 cut(s) 66, 601
Hin1II CATG 1 cut(s) 447
HinfI GANTC 2 cut(s) 314, 749
HpaII CCGG 2 cut(s) 66, 601
HphI GGTGA 4 cut(s) 12, 268, 624, 695
Hpy166II GTNNAC 2 cut(s) 264, 766
Hpy188I TCNGA 4 cut(s) 130, 176, 214, 286
Hpy188III TCNNGA 3 cut(s) 105, 475, 753
Hpy8I GTNNAC 2 cut(s) 264, 766
HpyCH4III ACNGT 3 cut(s) 301, 491, 683
HpyCH4IV ACGT 2 cut(s) 80, 436
HpyCH4V TGCA 6 cut(s) 77, 264, 365, 389, 447, 652
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 165, 270, 279, 371
HpyF3I CTNAG 2 cut(s) 283, 347
HpySE526I ACGT 2 cut(s) 80, 436
Hsp92II CATG 1 cut(s) 447
KpnI GGTACC 1 cut(s) 529
Kzo9I GATC 2 cut(s) 17, 328
LmnI GCTCC 3 cut(s) 176, 603, 611
Lsp1109I GCAGC 6 cut(s) 61, 122, 218, 379, 401, 459
LweI GCATC 1 cut(s) 376
MabI ACCWGGT 1 cut(s) 252
MaeI CTAG 1 cut(s) 324
MaeII ACGT 2 cut(s) 80, 436
MaeIII GTNAC 4 cut(s) 248, 295, 491, 591
MalI GATC 2 cut(s) 19, 330
MboI GATC 2 cut(s) 17, 328
MhlI GDGCHC 1 cut(s) 266
MluCI AATT 2 cut(s) 124, 691
MmeI TCCRAC 3 cut(s) 214, 662, 715
MnlI CCTC 6 cut(s) 220, 227, 252, 356, 640, 645
MslI CAYNNNNRTG 1 cut(s) 25
MspA1I CMGCKG 2 cut(s) 392, 554
MspI CCGG 2 cut(s) 66, 601
MspR9I CCNGG 1 cut(s) 254
MvaI CCWGG 1 cut(s) 254
MwoI GCNNNNNNNGC 5 cut(s) 71, 165, 270, 279, 371
NdeII GATC 2 cut(s) 17, 328
NlaIII CATG 1 cut(s) 447
NlaIV GGNNCC 2 cut(s) 527, 646
OliI CACNNNNGTG 1 cut(s) 25
PaeR7I CTCGAG 1 cut(s) 105
PfeI GAWTC 2 cut(s) 314, 749
PkrI GCNGC 7 cut(s) 76, 112, 208, 275, 391, 394, 449
PsiI TTATAA 2 cut(s) 308, 772
Psp6I CCWGG 1 cut(s) 252
PspGI CCWGG 1 cut(s) 252
PspN4I GGNNCC 2 cut(s) 527, 646
PspPI GGNCC 2 cut(s) 598, 644
PstNI CAGNNNCTG 1 cut(s) 212
PvuII CAGCTG 2 cut(s) 392, 554
RsaI GTAC 1 cut(s) 527
RsaNI GTAC 1 cut(s) 526
RseI CAYNNNNRTG 1 cut(s) 25
SatI GCNGC 7 cut(s) 75, 111, 207, 274, 390, 393, 448
Sau3AI GATC 2 cut(s) 17, 328
Sau96I GGNCC 2 cut(s) 598, 644
ScrFI CCNGG 1 cut(s) 254
SduI GDGCHC 1 cut(s) 266
SexAI ACCWGGT 1 cut(s) 252
SfaNI GCATC 1 cut(s) 376
SfcI CTRYAG 1 cut(s) 300
Sfr274I CTCGAG 1 cut(s) 105
SinI GGWCC 2 cut(s) 598, 644
SlaI CTCGAG 1 cut(s) 105
SmiMI CAYNNNNRTG 1 cut(s) 25
SmlI CTYRAG 1 cut(s) 105
SmoI CTYRAG 1 cut(s) 105
Sse9I AATT 2 cut(s) 124, 691
SsiI CCGC 2 cut(s) 159, 273
SspMI CTAG 1 cut(s) 324
StyD4I CCNGG 1 cut(s) 252
TaaI ACNGT 3 cut(s) 301, 491, 683
TaiI ACGT 2 cut(s) 83, 439
TaqI TCGA 2 cut(s) 106, 426
TasI AATT 2 cut(s) 124, 691
TauI GCSGC 1 cut(s) 276
TfiI GAWTC 2 cut(s) 314, 749
TscAI CASTG 2 cut(s) 66, 676
TseI GCWGC 6 cut(s) 74, 110, 206, 389, 392, 447
TspDTI ATGAA 1 cut(s) 432
TspRI CASTG 2 cut(s) 66, 676
VneI GTGCAC 1 cut(s) 262
VpaK11BI GGWCC 2 cut(s) 598, 644
XapI RAATTY 1 cut(s) 124
XhoI CTCGAG 1 cut(s) 105
XspI CTAG 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.